Morphological Responses Research Articles

Highly-efficient low-voltage electrodeposition of superhydrophobic diamond-like carbon films from N-methyl pyrrolidone

N-methyl pyrrolidone was proposed as a new carbon source for efficiently preparing the diamond-like carbon (DLC) films by a one-step electrodeposition technique at a low voltage. The responses of surface morphology, microstructure and surface wettability of the DLC films on the variation of the area ratio of anode to cathode were investigated in terms of current density in the process of electrodeposition. Especially, the dynamic behaviors of water droplets impacting on one of the superhydrophobic DLC films were studied in detail as a function of Weber number and inclined angle. Results revealed that either a larger or a smaller area ratio than 1.56 could facilitate the formation of hierarchical structure with more hydrocarbon features, which is in favor of creating superhydrophobicity with low surface adhesion. Further, it was found that a competitive strategy between deformation and movement depending on Weber number and inclined angle, where the movement is balanced by sliding with rolling, dominates the impact dynamics of water droplets. This work provides new insight and explanation for the efficient electrodeposition of DLC films at low voltage and the dynamic evolution of impacting droplets on the inclined superhydrophobic surface.

Leaf angle, light interception and chlorophyll contents of bean and soybean grown in integrated cropping systems

ABSTRACT This study aimed to evaluate the dynamics of solar radiation and the morphological and physiological responses of bean and soybean plants grown in two integrated cultivation systems and under full sun. Two experiments were conducted in southern Brazil, one with beans (in-season) and the other with soybeans (off-season). The experimental design used was randomised blocks in a bifactorial 2 × 2 arrangement with an additional treatment, characterised by two forest arrangements (6 × 6 m and 12 × 12 m); two forest species (Peltophorum dubium and Eucalyptus urograndis); and full sun, with three repetitions. The transmissivity of solar radiation, intercepted solar radiation, leaf inclination angle, light extinction coefficient (k), and chlorophyll content (a, b, total, and a/b ratio) were measured. The dynamics of solar radiation were modified by the arrangement of trees and the canopy characteristics of the forest species. Shading significantly influenced the levels of chlorophyll a, b, and total chlorophyll, while no variation was observed in the angle of inclination of the leaves. It is recommended to cultivate beans intercropped with the forest species E. urograndis in the 12 × 12 intercropped arrangement, while for soybean cultivation, intercropping with the two forest species studied in the 12 × 12 intercropped arrangement is recommended.

Predicting Survival of Metastatic Clear Cell Renal Cell Cancer Treated with VEGFR-TKI-Based Sequential Therapy.

(1) Objective: To develop a clinically useful nomogram that may provide a more individualized and accurate estimation of cancer-specific survival (CSS) for patients with clear-cell (CC) metastatic renal cell carcinoma (mRCC) treated with nephrectomy and vascular endothelial growth factor receptor-tyrosine kinase inhibitor (VEGFR-TKI)-based sequential therapy. (2) Methods: A prospectively maintained database of 145 patients with mRCC treated between 2008 and 2018 was analyzed to predict the CSS of patients receiving sunitinib and second- and third-line therapies according to current standards of practice. A nomogram based on four independent clinical predictors (Eastern Cooperative Oncology Group status, International Metastatic RCC Database Consortium score, the Morphology, Attenuation, Size and Structure criteria and Response Evaluation Criteria in Solid Tumors response criteria) was calculated. The corresponding 1- to 10-year CSS probabilities were then determined from the nomogram. (3) Results: The median age was 60 years (95% CI 57.9-61.4). The disease was metastatic at diagnosis in 59 (40.7%), and 86 (59.3%) developed metastasis during follow-up. Patients were followed for a median 48 (IQR 72; 95% CI 56-75.7) months after first-line VEGFR-TKI initiation. The concordance probability estimator value for the nomogram is 0.778 ± 0.02 (mean ± SE). (4) Conclusions: A nomogram to predict CSS in patients with CC mRCC that incorporates patient status, clinical risk classification and response criteria to first-line VEGFR-TKI at 3 months is presented. This new tool may be useful to clinicians assessing the risk and prognosis of patients with mRCC.

Resilience of turbid coral communities to marine heatwave

High sea surface temperatures recorded in summer 2021 introduced a unique opportunity for ‘real-time’ assessment of Exmouth Gulf turbid reef’s resilience to a marine heatwave event. Four sites along a turbidity and temperature gradient were surveyed during (March 2021) and after (October 2021) the event to assess bleaching rates (Bleaching Index = BI), differences in coral morphological responses to the heat wave, and post-event changes in benthic and coral community structure. Despite experiencing higher temperatures (> 30 °C) and Degree Heating Weeks (DHW = 8), the most turbid reef site, Somerville, displayed greater resilience to heat stress (BI = 14) compared to the “clear water” site, Bundegi (BI = 19.3), where temperatures never exceeded 30 °C (3 DHW). Our results also reveal that encrusting and massive corals, often considered more resilient to bleaching, displayed increased bleaching susceptibility at the turbid sites, potentially due to the synergistic effects of sedimentation and heat stress. In contrast, branching and foliose corals showed greater resilience to the heat wave in turbid water settings, while encrusting and branching corals exhibited lower resilience in the clearwater site. These findings highlight complex interactions between heat and reduced UV stress on turbid reefs potentially increasing resilience to bleaching but likely only for those coral morphologies that are not heavily impacted by sedimentation.

Human Leukocyte Antigen-DR and Cluster Designation 34: Double-negative Acute Myeloid Leukemias – Our Experience in Clinical Setting

Abstract Introduction: Dual negativity for cluster designation (CD) 34 and human leukocyte antigen (HLA)-DR antigen in leukemia panel is an important finding in clinical practice. The combination of this finding though seen classically in acute promyelocytic leukemia (APL) is not specific to it but can be seen in many other myeloid leukemias like the ones associated with nucleophosmin 1 (NPM1) or FMS-related tyrosine kinase 3 gene-internal tandem duplication (FLT3-ITD) abnormalities. It is important to identify APL from non-APL leukemia as treatment and prognosis vary. Aims and Objectives: We analyzed CD 34 and HLA-DR dual-negative acute myeloid leukemia (AML) cases presented to us over 2.5 years (26 cases) and segregated them into APL and non-APL groups to study their morphological/flow cytometric and cytogenetic profile and correlation with treatment response. Materials and Methods: It was a prospective study including all newly diagnosed AMLs showing CD 34 and HLA-DR dual negativity in flow cytometry profile. Cases in which complete information/cytogenetics/molecular profile were not available, were excluded from the study. The patients were followed up till the end of induction to look for morphological response to induction therapy. The clinical and treatment records were pulled out from the patient database after ethical committee clearance. Results: A total of 139 new AML cases were encountered during the study period out of which 28 (20%) were found to be dual negative for CD34 and HLA-DR. The non-APL group showed higher mean age, TLC at baseline, variable morphology, and more number of aberrant expression on flow cytometry profile. No significant difference was noted in terms of gender, presence of hepatosplenomegaly, DIC as complication, or percentage of blasts/blasts equivalent at baseline. Our study found that while all APL patients had PML-RARA, the associated molecular abnormalities were much lesser as compared to the non-APL group which showed a variety of abnormalities such as NPM1, FLT3, RAS mutation, and mutations involving epigenetic modifiers. The response rate to induction therapy was significantly lower in the non-APL group as compared to the APL group. Conclusion: The non-APL myeloid leukemia which is dual negative is commonly found to be associated with cup-shaped morphology, NPM, or FLT3-ITD mutation along with other abnormalities. These patients were older in age, showed higher TLC and higher blast counts, associated with poorer response to induction therapy as compared to the APL group.

The curvilinear responses of biomass accumulation and root morphology to a soil salt-nitrogen environment reflect the phytodesalination capability of the euhalophyte Suaeda salsa L.

Under the sufficient nitrogen supply, it is of great significance to investigate the law of biomass allocation, root morphological traits, and the salt absorption capacity of euhalophytes to evaluate their biological desalination in saline soil. Although the curvilinear responses of biomass accumulation and root morphology in response to soil salinity have been recognized, these perceptions are still confined to the descriptions of inter-treatment population changes and lack details on biomass allocation in organs at an individual level. In this study, Suaeda salsa was grown in root boxes across a range of soil salt levels. The study showed that their growth and development were significantly affected by soil soluble salts. The law of biomass allocation was described as follows: increased soil soluble salts significantly increased the leaf mass ratio and decreased the stem mass ratio, and slightly increased the root mass ratio among treatments. For individuals at each treatment, leaf mass ratio > stem mass ratio > root mass ratio, except in the control treatment at the flower bud and fruit stages. Biomass responses of the control treatment indicated that salt was not rigorously required for Suaeda salsa in the presence of an adequate nitrogen supply, as verified by the correlation between biomass, nitrogen, and soil soluble salt. Salt could significantly inhibit the growth of Suaeda salsa (P<0.01), whereas nitrogen could significantly promote its growth (P<0.01). Root morphology in response to soil soluble salts showed that salt acquisition by the root was highest at a salt level of 0.70%, which corresponds to light saline soil. Consequently, we conclude that phytodesalination by Suaeda salsa was optimal in the light saline soil, followed by moderate saline soil.

Engineered nanofibrillar collagen with tunable biophysical properties for myogenic, endothelial, and osteogenic cell guidance

A goal of regenerative engineering is the rational design of materials to restore the structure-function relationships that drive reparative programs in damaged tissues. Despite the widespread use of extracellular matrices for engineering tissues, their application has been limited by a narrow range of tunable features. The primary objective of this study is to develop a versatile platform for evaluating tissue-specific cellular interactions using Type I collagen scaffolds with highly tunable biophysical properties. The kinetics of collagen fibrillogenesis were modulated through a combination of varied shear rate and pH during neutralization, to achieve a broad range of fibril anisotropy, porosity, diameter, and storage modulus. The role that each of these properties play in guiding muscle, bone, and vascular cell types was comprehensively identified, and informed the in vitro generation of three distinct musculoskeletal engineered constructs. Myogenesis was highly regulated by smaller fibrils and larger storage moduli, endothelial inflammatory phenotype was predominantly guided by fibril anisotropy, and osteogenesis was enhanced by highly porous collagen with larger fibrils. This study introduces a novel approach for dynamically modulating Type I collagen materials and provides a robust platform for investigating cell-material interactions, offering insights for the future rational design of tissue-specific regenerative biomaterials. Statement of significanceThe biophysical properties of regenerative materials facilitate key cell-substrate interactions that can guide the morphology, phenotype, and biological response of cells. In this study, we describe the fabrication of an engineered collagen hydrogel that can be modified to exhibit control over a wide range of biophysical features, including fibril organization and size, nanoscale porosity, and mechanics. We identified the unique combination of collagen features that optimally promote regenerative muscle, bone, and vascular cell types while also delineating the properties that hinder these same cellular responses. This study presents a highly accessible method to control the biophysical properties of collagen hydrogels that can be adapted for a broad range of tissue engineering and regenerative applications.

Morphological and Physiological Response of Maize (Zea mays L.) to Drought Stress during Reproductive Stage

Maize is among the most significant crops in the world regarding production and yield, but it is highly sensitive to drought, which reduces the growth, photosynthetic efficiency, grain quality, and yield production of a plant. Quantum yield efficiency of photosystem II is a critical photosynthetic component that is susceptible to drought stress. This study intended to investigate the effects of drought stress on growth and morpho-physiological parameters using three maize hybrids (‘P-3011w’, ‘P-3092’ and ‘iku20’) with contrasting soil moisture contents (100%, 40%) at the pre-flowering stage. The stress treatment (40%) was initiated at stage V7, for a period of 15 days; the experimental units were established in a completely randomized design with split-plot arrangement along with three repetitions in 42 L pots using a substrate of peat moss, black soil and poultry manure (1:2:1). The morphological, growth-related and physiological parameters were assessed, including chlorophyll fluorescence (Fv/Fm), which was measured using a LiCor-6400-40 fluorometer. The results showed that all morphological, growth-related and physiological variables decreased under drought stress during the reproductive stage, with the exception of leaf temperature and intercellular CO2 concentration, which increased by 12% and 54%, respectively. Drought stress significantly reduced the photosynthetic chlorophyll fluorescence (43%), due to damage to photosystem II. The lowest percentage of damage to photosystem II (34%) was observed in the iku20 genotype. In contrast, P-3011w and P-3092 had the highest levels of significantly similar damage (49% and 46%, respectively). The correlation analysis showed a highly positive interaction of chlorophyll fluorescence (Fv/Fm) with net photosynthetic rate and stomatal conductance under drought conditions, and multiple regression analysis revealed that the maximum effect on net photosynthetic rate under drought was due to the damage it caused to photosystem II. Thus, iku20 might have a tendency to be able to withstand drought stress in the dry northeast region of Mexico. Overall, we concluded that the photosystem II was negatively impacted by drought stress thus causing a reduction in all physiological, morphological and growth-related variables.

Change in Femoral Cartilage Cross-Sectional Area After Aerobic and Resistance Exercise.

We compared the immediate response and recovery of femoral cartilage morphology following aerobic or resistance exercise to a control condition. Fifteen healthy young males (23.9 years; 170.1 cm; 69.7 kg) visited the laboratory three separate days and randomly performed one of the 30-min exercise aerobic exercises (treadmill running), resistance exercises (leg presses, back squats, and knee extensions), or seated rest as the control, each followed by the 50-min recovery. Ultrasonographic images of the femoral cartilage cross-sectional area (CSA) were obtained before and after exercise and every 5 min thereafter. To test exercise effects over time, a mixed model analysis of variance and Tukey-Kramer post-hoc tests were performed (p<0.05). The femoral cartilage CSA was different (condition×time: F34,742=4.30, p<0.0001) and the femoral cartilage CSA was decreased after the aerobic (-5.8%, p<0.0001) and the resistance (-3.4%, p=0.04) exercises compared to the pre-exercise levels. Deformed femoral cartilage CSA took 35 and 10 min to return to the pre-exercise levels after aerobic and resistance exercises (p+>+0.09), respectively. Thirty minutes of moderate exertion performing aerobic or resistance exercises immediately reduced the femoral cartilage CSA. A rest period ranging from 10 to 35 min was required for cartilage recovery after weight-bearing exercises.

Does habitat modification impact morphology, performance, and inflammatory responses in an amphibian with limited dispersal capacity (Lisssotriton helveticus)?

The environment of an organism exerts selective pressures that affect mobility, feeding, reproduction as well as predator-prey and conspecific interactions. Land use changes induced by human activities modify these selective pressures and may result in the adaptation of organisms. Amphibians are ectotherms that typically show a biphasic life cycle with an aquatic and terrestrial phase, which makes them particularly sensitive to environmental change. We studied the impact of habitat modifications on palmate newt populations in the Ile de France region across four types of habitats: urban, mixed, agricultural, and natural with at least two replicates for each habitat type. We measured the morphology of newts using callipers, quantified maximal running and swimming speed and acceleration using high-speed video recordings, and quantified the swelling of the hind limb linked to an inflammatory reaction. Our results show that in urban habitats, newts are larger and heavier and have a better body condition. Females, moreover, have a larger head in natural habitats, possibly due to diet specialisation of females during the breeding season. In mixed and agricultural habitats, newts have longer limbs and show a tendency to run faster, possibly associated with the selective pressures on movement in mixed habitats. Differences in inflammatory responses were observed between sexes but not habitat types. Overall, our results show differences in morphology and trends for differences in performance in newts living in different habitats suggesting that animals are adapting to human-induced changes in their environment.

Preparation and characterization of chromium oxide thin films: The response of morphology, crystal structure, and electrical properties to oxygen pressure under magnetron sputtering

This study focuses on Cr2O3 prepared via reactive magnetron sputtering, a direct deposition technique that significantly impacts the microstructure and optoelectronic properties of the films. Reactive sputtering alters grain size, density, and surface morphology, which in turn affects the structural order and stoichiometry. By adjusting the ratio of process gases, particularly the oxygen partial pressure, the stoichiometry of the deposited films was controlled. This is crucial for managing the oxidation state of chromium and the concentration of free electrons. An optimal oxygen partial pressure of 75 % was identified, substantially reducing oxygen interstitial defects in the CrOx materials and enhancing conductivity by nearly two orders of magnitude. Additionally, increasing the oxygen partial pressure helped integrate more oxygen atoms into the lattice, transitioning the electronic structure from a metallic state in CrO2 to a semiconductive state in CrO3. This fine-tuning of oxygen doping not only adjusts carrier concentrations but also optimizes the photoelectric properties of the materials, achieving a tailored high band gap of 3.34 eV. This study highlights the potential of reactive magnetron sputtering to customize semiconductor materials through oxygen doping, offering a novel approach to enhance the versatility and application range of chromium oxide materials in advanced technological applications.

Exploring the Therapeutic Potential of Silicon and its Impact on Crude Plant Drug Production in North East India

Silicon (Si) exhibits a beneficial impact in mitigating both biotic and abiotic stress, yet its potential significance in the context of medicinal plant and crude plant drug production, particularly in the strongly acidic soils of North East (NE) India, remains underreported. This study aimed to assess the effects of Si application on medicinal plants, focusing on growth, physico-chemical and biological attributes under stressful conditions.The study highlighted the benefits of Si application, particularly through foliar application, on medicinal plants such as lemongrass [Cymbopogon flexuosus (Steud) Wats], ginseng (Panax ginseng), coriander (Coriandrum Sativum L.) and bananas. Foliar Si application demonstrated improvements in plant growth and yield, employing morphological, physiological and biological responses in lemongrass and ginseng while enhancing the growth and yield of coriander by improving relative water content, total phenolic and total flavonoid content under water stress. Si applications also resulted in increased fresh and dry weight of bananas, alongside improvements in biochemical parameters, like increased starch and total sugar content and delay in banana ripening through the reduction of cellulose and amylase activity. The study underscores the effectiveness of Si in enhancing the growth and yield of medicinal plants. Within the context of NE India, this report draws attention to the considerable challenges faced in cultivating well-suited medicinal plants and crude plant drugs, crucial for herbal healthcare and the future well-being of humanity. Consequently, Si’s role in fostering t

Diversity of microglial transcriptional responses during opioid exposure and neuropathic pain.

Microglia take on an altered morphology during chronic opioid treatment. This morphological change is broadly used to identify the activated microglial state associated with opioid side effects, including tolerance and opioid-induced hyperalgesia (OIH). Microglia display similar morphological responses in the spinal cord after peripheral nerve injury (PNI). Consistent with this observation, functional studies have suggested that microglia activated by opioids or PNI engage common molecular mechanisms to induce hypersensitivity. In this article, we conducted deep RNA sequencing (RNA-seq) and morphological analysis of spinal cord microglia in male mice to comprehensively interrogate transcriptional states and mechanistic commonality between multiple models of OIH and PNI. After PNI, we identify an early proliferative transcriptional event across models that precedes the upregulation of histological markers of microglial activation. However, we found no proliferative transcriptional response associated with opioid-induced microglial activation, consistent with histological data, indicating that the number of microglia remains stable during morphine treatment, whereas their morphological response differs from PNI models. Collectively, these results establish the diversity of pain-associated microglial transcriptomic responses and point towards the targeting of distinct insult-specific microglial responses to treat OIH, PNI, or other central nervous system pathologies.

Using a thermal gradient table to study plant temperature signalling and response across a temperature spectrum

Plants must cope with ever-changing temperature conditions in their environment. In many plant species, suboptimal high and low temperatures can induce adaptive mechanisms that allow optimal performance. Thermomorphogenesis is the acclimation to high ambient temperature, whereas cold acclimation refers to the acquisition of cold tolerance following a period of low temperatures. The molecular mechanisms underlying thermomorphogenesis and cold acclimation are increasingly well understood but neither signalling components that have an apparent role in acclimation to both cold and warmth, nor factors determining dose-responsiveness, are currently well defined. This can be explained in part by practical limitations, as applying temperature gradients requires the use of multiple growth conditions simultaneously, usually unavailable in research laboratories. Here we demonstrate that commercially available thermal gradient tables can be used to grow and assess plants over a defined and adjustable steep temperature gradient within one experiment. We describe technical and thermodynamic aspects and provide considerations for plant growth and treatment. We show that plants display the expected morphological, physiological, developmental and molecular responses that are typically associated with high temperature and cold acclimation. This includes temperature dose-response effects on seed germination, hypocotyl elongation, leaf development, hyponasty, rosette growth, temperature marker gene expression, stomatal conductance, chlorophyll content, ion leakage and hydrogen peroxide levels. In conclusion, thermal gradient table systems enable standardized and predictable environments to study plant responses to varying temperature regimes and can be swiftly implemented in research on temperature signalling and response.

Influence of annealing temperature on the structure, morphology, optical property and antibacterial response of phytochemicals-assisted synthesized zinc oxide nanoparticles

Abstract Antibiotic overuse has caused a variety of bacterial pathogens to develop new resistance mechanisms. As a result, discovering an appropriate replacement for the standard antibiotics has become an immediate concern. The present work demonstrates a facile, eco-friendly and economical method for the synthesis of hexagonal wurtzite zinc oxide nanoparticles (ω-ZONPs) using the ethanolic extract of triphala. Gas chromatography–mass spectrometry analysis of the triphala extract proved the presence of certain secondary metabolites, which aids in the formation of ω-ZONPs. The influence of annealing temperature on the antibacterial action of as-synthesized ω-ZONPs was studied for three different annealing temperatures. X-ray diffraction, dynamic light scattering, field emission electron microscopy and energy dispersive X-ray spectroscopy analyses were used to examine the impact of annealing temperature on the structure, particle size and morphology of ω-ZONPs. Fourier transform infrared spectra revealed the change in intensity of the characteristic peaks in ω-ZONPs with different annealing temperatures. From UV–Visible diffuse reflectance spectroscopy, variation in the band gap of ω-ZONPs with increasing annealing temperature was detected. Kirby Bauer disc diffusion was adopted to examine the antibacterial potential of ω-ZONPs against bacterial strains such as Staphylococcus aureus, Enterococcus faecium, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. The ω-ZONPs annealed at 200 °C inhibited the growth of three bacterial pathogens, E. coli, B. subtilis and P. aeruginosa and exhibited effective antibacterial activity in comparison with ω-ZONPs annealed at relatively high temperatures. Thus, the antibacterial potential of ω-ZONPs could be further explored as disease controlling agents and such prototypes could be made available for commercial mass production.

Drought Resistance in Wheat (Triticum aestivum L)

Wheat is a grain crop that is farmed all over the world for bread, chapati, and biscuits. Specific wheat breeding and quality development projects that result in high-yielding, genetically superior, disease resistant varieties of desirable quality that are adaptive to growing conditions. Drought is one of the most important factors affecting crop productivity and yield. Drought stress has a variety of effects on crops, including molecular, physiological, biochemical responses and morphological. Wheat has numerous genes that control drought tolerance and produce a variety of proteins and enzymes, including Responsive to abscisic acid (Rab), late embryogenesis abundant (lea), proline, ligase, rubisco, carbohydrates and antioxidant enzyme (GST). The influence of water shortage on the morphological, biochemical, molecular and physiological responses of wheat to possible drought stress losses was the subject of this review research. Drought is a state in which soil moisture is decreased, resulting in a variety of agricultural changes. Drought reduces crop yield and leads to crop failure. It has an impact on morphology, agricultural productivity and other factors. The vegetative, reproductive which are essential growth stages are affected. Some plants have mechanism to tolerate those conditions. Nearly 50% of the crop production has decreased due to droughts. The various approaches like Quantitative trait locus (QTL) are employed for drought tolerance. Punjab produces 125 metric tonnes of wheat (in lakh metric tonnes), Madhya Pradesh(113.38MT), Haryana (70.65 MT) and Uttar Pradesh (70.65 MT) are the averages (20.39MT) whereas Rajasthan (10.63 MT). The review gives details on latest innovations for the development of resistant genotypes.

Drought Stress Tolerance in Rice: Physiological and Biochemical Insights

Rice (Oryza sativa L.), an important food crop, necessitates more water to complete its life cycle than other crops. Therefore, there is a serious risk to rice output due to water-related stress. Drought stress results in morphological changes, including the inhibition of seed germination, reduced seeding growth, leaf area index, flag leaf area, increased leaf rolling, as well as the decrement of yield traits, such as plant height, plant biomass, number of tillers, and 1000-grain yield. Stress also causes the formation of reactive oxygen species (ROS) such as O2−, H2O2, and OH−, which promote oxidative stress in plants and cause oxidative damage. The process of oxidative degradation owing to water stress produces cell damage and a reduction in nutrient intake, photosynthetic rate, leaf area, RWC, WUE, and stomatal closure, which may be responsible for the decrement of the transpiration rate and plant dry matter under decreasing soil moisture. Plants have the ability to produce antioxidant species that can either be enzymatic (SOD, POD, CAT, GPX, APX) or non-enzymatic (AsA, GSH) in nature to overcome oxidative stress. During drought, several biochemical osmoprotectants, like proline, polyamines, and sugars, can be accumulated, which can enhance drought tolerance in rice. To meet the demands of an ever-growing population with diminishing water resources, it is necessary to have crop varieties that are highly adapted to dry environments, and it may also involve adopting some mitigation strategies. This study aims to assess the varying morphological, physiological, and biochemical responses of the rice plant to drought, and the various methods for alleviating drought stress.

Channel changes during and after extreme floods in two catchments of the Northern Apennines (Italy)

The Nure and Trebbia catchments were affected by an extreme flood event on 13-14th September 2015. This study investigates the morphological responses to the flood of the Nure and Trebbia rivers and 18 tributaries with the aims to: i) quantify channel changes in width and pattern; (ii) identify the morphological and hydrological factors which might have driven channel response; (iii) quantify channel changes which have occurred after the flood event.Channel changes were characterized by field surveys and geomorphological analysis of multi-temporal orthophotos acquired before (2011), immediately after the flood (2015) and in 2020.In the tributaries, reach-scale channel widths after the flood reached up to 15 times their pre-flood values, whereas in the main channels the post/pre-flood width ratio attained a maximum value of 4. Augmented channel width in the main rivers was mostly associated with banks and islands erosion, whereas in the tributaries it was also due to deposition of coarse sediments onto the former floodplains. Islands were swept away in the main channels while both island erosion and formation occurred in the tributaries. In terms of channel pattern, pre-flood single-thread reaches displayed mainly multi-thread morphologies after the flood, mostly in response to a sudden input of coarse sediment supply. In the 5 yr following the flood, channels slightly narrowed but still remained wider than in 2011. The narrower reaches before the flood resulted to be the most sensitive to changes even some years after the flood.Statistical analyses between the channel width changes and a series of variables showed significant positive correlations with confinement index, channel slope, and the local storm rainfall depth. This study confirms how channel widening during large floods – usually neglected in flood hazard mapping and river basin management – is a very important process which must be considered in flood hazard assessment in mountain rivers.

Ross 708 broiler small intestine morphology and immunity improvements in response to in ovo Marek's Disease vaccine administration alone or in conjunction with in ovo and dietary supplemental calcifediol

Investigations were performed to determine the systemic immune and small intestine (SI) morphological responses of Ross 708 broilers to the Marek's Disease vaccine (MDV) administered alone or in conjunction with the in ovo and dietary administration of calcifediol (25OHD3). Live embryonated hatching eggs were assigned at random to 3 in ovo treatments at 18 d of incubation. Pre-specified in ovo treatments were: commercial MDV-alone-injected (50 µL) or commercial MDV containing 1.2 (MDV+25OHD3-1.2) or 2.4 (MDV+25OHD3-2.4) μg of 25OHD3. A non-injected control treatment was also included. For the growing phase, broilers received a commercial diet containing 250 IU of vitamin D3 /kg (control) or a commercial diet supplemented with 2,760 IU of 25OHD3 /kg (Hy-D diet). For determination of serum IgG, nitric oxide, and α-1-acid glycoprotein (AGP) at 14 and 40 d of age (doa), blood was collected from 1 bird per pen (48 total). In the duodenum, jejunum, and ileum of the same bird, villus length (VL), crypt depth (CD), VL to CD ratio (VCR), and villus surface area were also determined. There were no significant dietary x in ovo treatment interactions for any of the variables examined. However, birds fed Hy-D diets had lower serum AGP levels at 14 doa when compared to those fed un-supplemented commercial diets. Additionally, at 40 doa, birds in the MDV+25OHD3-1.2 and MDV+25OHD3-2.4 treatments experienced a decrease in serum AGP in comparison to those belonging to the non-injected and MDV-alone treatment groups. A higher jejunal VCR was observed at 14 and 40 doa in birds that belonged to the MDV+25OHD3-1.2 treatment when compared to those in the non-injected and MDV-alone treatment groups, and dietary Hy-D increased the VL of the duodenum and jejunum in birds at 14 and 40 doa when compared to those fed the commercial diet. In conclusion, both dietary or in ovo administration of 25OHD3 lowered inflammatory reactions and improved the SI morphology of broilers that were in ovo-injected with the MDV.

Contrasting morphometric responses to increasing urbanisation in congeneric sparrow species

Increased urbanisation influences the morphometric traits of various species, often resulting in urban individuals being smaller than their non-urban counterparts. Urbanisation can affect fundamental eco-evolutionary patterns and impact species’ ability to adapt to and occupy rapidly changing environments through morphological changes. We investigated the morphometric responses of two passerine species, the non-native house sparrow (Passer domesticus) and its native congener, the Cape sparrow (Passer melanurus), along gradients of spatial and temporal urbanisation in South Africa over a 52-year period. The house sparrow was significantly heavier, larger and in better condition with increasing urban infrastructure and lower urban vegetation cover, while the Cape sparrow showed opposing trends along these gradients. Temporally, the house sparrow’s body mass increased consistently over the 52-year study period, suggesting changes in morphology were concomitant with increasing urbanisation over time. This study demonstrates distinct differences in the morphological responses of the non-native house sparrow and the native Cape sparrow to increasing urban development. These morphological responses may also underpin community-level changes caused by urbanisation, enhancing the capabilities of non-native species to thrive over their native counterparts in these environments.