Morphology Profile Research Articles

A Cluster of Diutina catenulata Funguria in Patients with Coronavirus Disease 2019 (COVID-19) Hospitalized in a Tertiary Reference Hospital from Rio de Janeiro, Brazil.

During the COVID-19 pandemic, fungal infections, especially pulmonary aspergillosis, mucormycosis, and invasive candidiasis, have emerged as a significant health concern. Beyond Candida albicans, the most common cause of invasive candidiasis, other rare ascomycetous yeast species have been described in tertiary care units, potentially posing a broader health threat. We have isolated, from September 2020 to June 2021, nine Diutina catenulata strains from urine samples of six patients. This was intriguing as this fungus had not been previously identified in our institution, nor after June 2021. Therefore, we decided to outline the clinical features of the patients with this rare pathogen, to describe phenotypic characteristics, including antifungal susceptibility profiles, of this yeast species and to identify the genetic makeup through whole-genome sequencing analysis to evaluate if this was a cluster of genetically similar D. catenulata isolates in our institution. The strains were identified through MALDI-TOF MS analyses and Sanger sequencing of two rDNA regions. All patients yielding D. catenulata from urine samples needed ventilator support and used urinary catheters during hospitalization for treatment of COVID-19. None of them had received COVID-19 vaccines. Morphological and biochemical profiles of the nine strains were largely consistent, although fluconazole susceptibility varied, ranging from 4 to 32μg/mL. Phylogenomic analysis revealed minimal genetic variation among the isolates, with low intrapopulation variation, supported by the identification of only 84 SNPs across all strains. Therefore, we propose that the yeast strains isolated were part of a cluster of D. catenulata funguria in the context of COVID-19.

Fully defined NGN2 neuron protocol reveals diverse signatures of neuronal maturation

NGN2-driven induced pluripotent stem cell (iPSC)-to-neuron conversion is a popular method for human neurological disease modeling. In this study, we present a standardized approach for generating neurons utilizing clonal, targeted-engineered iPSC lines with defined reagents. We demonstrate consistent production of excitatory neurons at scale and long-term maintenance for at least 150days. Temporal omics, electrophysiological, and morphological profiling indicate continued maturation to postnatal-like neurons. Quantitative characterizations through transcriptomic, imaging, and functional assays reveal coordinated actions of multiple pathways that drive neuronal maturation. We also show the expression of disease-related genes in these neurons to demonstrate the relevance of our protocol for modeling neurological disorders. Finally, we demonstrate efficient generation of NGN2-integrated iPSC lines. These workflows, profiling data, and functional characterizations enable the development of reproducible human invitro models of neurological disorders.

Altered placental morphology and metabolomic profile in association with metabolically healthy obesity

Altered placental morphology and metabolomic profile in association with metabolically healthy obesity

Influence of N2 admixture on mode transition of discharge in N2–Ar helicon plasma

Effects of N2 admixture on multiple wave modes and transitions were investigated in N2–Ar helicon plasma under fixed input power and magnetic field. The structures of helicon waves were measured by a B-dot probe to verify the different eigenmodes. The experimental results show that the plasma morphology, emission spectrum, and spatial profile change significantly during mode transitions with the N2–Ar ratio. The calculated results from the pressure balance model indicate that the densities of species N2, N+, Ar, and Ar+ will change largely during mode transition around some specific N2 percentages, which will help to improve the application of N2–Ar helicon plasma in material processing greatly.

Assessing biological invasion predatory impacts through interaction strengths and morphological trophic profiling

AbstractBiological invasions are a major stressor on ecosystems worldwide, but tools to predict their predatory impact remain limited. Here, we quantified invader impacts using two complementary approaches: functional responses (to reveal per capita and multiple predator interaction strengths) and ecomorphology (to reveal trophic profiles and competitive overlap). We compared Mozambique tilapia Oreochromis mossambicus, a native southern African cichlid, and a near-trophically analogous invasive congener, the Nile tilapia Oreochromis niloticus. Both Nile tilapia and Mozambique tilapia exhibited a potentially prey population destabilizing Type II functional response. In both single and multiple predator pairings, invasive Nile tilapia had significantly greater prey consumption rates than native Mozambique tilapia, and thereby a greater predatory impact than its native congeneric. Attack rates were greater for Nile tilapia than Mozambique tilapia, with both species showing more similar handling times and maximum feeding rates. No evidence for multiple predator effects was detected within or between these species, and therefore impacts of both species increased additively in the presence of conspecific or heterospecific competitors. Morphological trait analyses found general differences between these two species, with the invasive Nile tilapia having distinctively larger lower jaw closing force, gill resistance and gill raker length, which facilitated greater feeding capacities over the native species. Trophic profiles predicted using morphological trait differences showed high dietary overlap and served as evidence for potential exploitative competition between the two species. These results reveal superior interaction strengths and ecomorphological trait profiles of an invasive over native species which could influence impact and native species replacement dynamics. Novel applications of functional response and ecomorphology provide complementary insights into predatory and competitive impacts from invasive species, aiding impact prediction across environmental contexts.

Morphological profiling and DNA barcoding revealed genetic diversity and phylogeny of Mentha species cultivated in Pakistan

Morphological profiling and DNA barcoding revealed genetic diversity and phylogeny of Mentha species cultivated in Pakistan

Valorisation of bamboo stalk waste: eco-friendly synthesis and characterisation of activated carbon monolith

ABSTRACT In contemporary waste management, the escalating volumes of bamboo stalk waste present a pressing environmental challenge, necessitating innovative and sustainable solutions. This research examined the properties of activated carbon monolith (ACM) derived from bamboo stalk (BS) using a facile method. Polystyrene resin and KOH were employed as an eco-binder and activator in the monolith synthesis, respectively. Analytical methods were employed to characterise the mechanical, morphological, elemental, and textual profiles of the monolith obtained. The study's results indicated the creation of a monolith characterised by a rough, amorphous shape firmly secured by the binder. It revealed a BET surface area measuring 265 m2/g and a pore diameter of 2.8 nm. The monolith displayed various irregular void pores, forming a mesoporous morphological network, along with sporadically distributed slivery-white dot-like particles on its heterogeneous surface. EDX analysis also revealed the BS-ACM is largely composed of carbon (77%), potassium (11%), oxygen (8%), and trace amounts of aluminium, gold, iron, and plutonium. The compressive strength test indicated a peak force of 21,536 N before any significant signs of appreciable deformation were observed, with the subsequent estimation of Young's modulus at 8.34 MPa. Beyond advancing bamboo waste valorisation, the research aligns with circular economy principles and sustainable practices, presenting a promising pathway for developing eco-friendly materials and contributing to a more sustainable and resource-efficient future.

Breast phyllodes tumour with epithelioid feature predisposes to malignant transformation

AimsPhyllodes tumours (PTs) are relatively common fibroepithelial tumours comprising epithelial and stromal component. Usually, PTs show a spindle cell morphology with a fibroblast phenotype, while some tumour cells exhibit epithelioid...

Exploring the adsorption of 4,4’-[oxalylbis(imino)]bis(2-hydroxybenzoic acid) layer at steel/biogasoline interface by the combined electrical-optical-simulation approach

Reactions at the steel/biogasoline interface trigger the adsorption of 4,4’-[Oxalylbis(imino)]bis(2-hydroxybenzoic Acid) (ODA) layer on the steel surface, thereby activating a mechanism that inhibited the early reactions. Exploring the conditionally deposited ODA layer requires a combined approach, including electrical, optical, and simulation techniques to track the film development and coating characteristics over time, and with the assistance of atomic force microscopy, quantum chemistry (DFT), and molecular dynamics (MD) simulations to reveal the adsorption mechanism of the ODA layer at steel/biogasoline interface. The four experimental ODA concentrations were conducted, related to the simulated un-coverage, undersaturated-, saturated-, and oversaturated-coverage model of the adsorbate on the adsorbent. The EC-RS data examines surface compositions and their distribution, coating/solution interface, and coating/substrate adhesion by, respectively, Raman spectroscopy (RS), electrochemical impedance spectroscopy (EIS), and current density—potential (I-V) scan. Namely, RS pointed out that an organic layer was established when ODA was added to the simulated biogasoline. EIS results revealed insulator behaviors of the ODA layer at the solid–liquid interface, limiting the charge transfer between the steel substrate and the biogasoline. I-V results showed an increase in surface current density and a decrease in surface polarization resistance of the coating with the rise in ODA concentration. The AFM morphology profile verified the degradation of the sample’s surface when exposed to biogasoline and the minimization of surface damage by ODA addition through adsorption. The simulation findings revealed that the adsorption of ODA on steel preferred physisorption, reaching the most stable state at a specified temperature and ODA concentration. The adsorption mechanism follows the Generalized Langmuir isotherm. The adsorbate (ODA molecules) can produce a transition phase with the steel substrate surface, which modifies the surface thermodynamic characteristics. The combined electro-optical-simulation technique can be applied to investigate various surface phenomena (reactions, catalyzes, adsorption). It especially helps to understand the protective mechanism of inhibitors in different media.

Unveiling Na‐ion storage mechanism and interface property of layered perovskite Bi2TiO4F2@rGO anode in ether‐based electrolyte

AbstractTo unveil the charge storage mechanisms and interface properties of electrode materials is very challenging for Na‐ion storage. In this work, we report that the novel layered perovskite Bi2TiO4F2@reduced graphene oxides (BTOF@rGO) serves as a promising anode for Na‐ion storage in an ether‐based electrolyte, which exhibits much better electrochemical performance than in an ester‐based electrolyte. Interestingly, BTOF@rGO possesses a prominent specific capacity of 458.3–102 mAh g−1/0.02–1 A g−1 and a high initial coulombic efficiency (ICE) of 70.3%. Cross‐sectional morphology and depth profile surface chemistry indicate not only a denser reactive interfacial layer but also a superior solid electrolyte interface film containing a higher proportion of inorganic components, which accelerates Na+ migration and is an essential factor for the improvement of ICE and other electrochemical properties. Electrochemical tests and ex situ measurements demonstrate the triple hybridization Na‐ion storage mechanism of conversion, alloying, and intercalation for BTOF@rGO in the ether‐based electrolyte. Furthermore, the Na‐ion batteries assembled with the BTOF@rGO anode and the commercial Na3V2(PO4)2F3@C cathode exhibit remarkable energy densities and power densities. Overall, the work shows deep insights on developing advanced electrode materials for efficient Na‐ion storage.

Alteration in the Morphological and Transcriptomic Profiles of Acinetobacter baumannii after Exposure to Colistin.

Acinetobacter baumannii is often highly resistant to multiple antimicrobials, posing a risk of treatment failure, and colistin is a "last resort" for treatment of the bacterial infection. However, colistin resistance is easily developed when the bacteria are exposed to the drug, and a comprehensive analysis of colistin-mediated changes in colistin-susceptible and -resistant A. baumannii is needed. In this study, using an isogenic pair of colistin-susceptible and -resistant A. baumannii isolates, alterations in morphologic and transcriptomic characteristics associated with colistin resistance were revealed. Whole-genome sequencing showed that the resistant isolate harbored a PmrBL208F mutation conferring colistin resistance, and all other single-nucleotide alterations were located in intergenic regions. Using scanning electron microscopy, it was determined that the colistin-resistant mutant had a shorter cell length than the parental isolate, and filamented cells were found when both isolates were exposed to the inhibitory concentration of colistin. When the isolates were treated with inhibitory concentrations of colistin, more than 80% of the genes were upregulated, including genes associated with antioxidative stress response pathways. The results elucidate the morphological difference between the colistin-susceptible and -resistant isolates and different colistin-mediated responses in A. baumannii isolates depending on their susceptibility to this drug.

Exploring the relationship between rearing system and carcass traits of Danzhou chicken: a microbial perspective

This study investigated the effects of free-range (FR) and cage-rearing (CR) systems on intestinal health, carcass traits, and microbial diversity in the Danzhou chicken breed. Two groups of 125 hens in each group, aged 42 wk, were reared under FR and CR systems. At 50 wk, 50 hens from each group were randomly selected for carcass analysis and 10 hens for intestinal morphology and microbiota profiling. Results indicated a significant increase in villus height (VH) in the duodenum (P < 0.05), jejunum (P < 0.01), and ileum (P < 0.001) of the CR group. Additionally, the ratio of VH to crypt depth (VR) significantly (P < 0.001) increased in the jejunum, while crypt depth (CD) decreased significantly (P < 0.001) in the same section in the CR group. Carcass traits, including dress weight (DW), eviscerated with giblet weight (EGW), eviscerated weight (EW), and leg muscle weight (LW) significantly improved (P < 0.05) in the CR group. Microbial diversity showed significant β-diversity differences, with Lactobacillus, Enterococcus, and Oxalobacteraceae as dominant biomarkers in the CR group. Conversely, Actinomycetaceae, Erysipelotrichaceae, Coriobacteriaceae, Eubacterium, Actinomyces, Scardovia, and Lachnospiraceae were dominant in the FG group. Correlation analysis showed duodenum Lactobacillus was positively correlated with VH (P < 0.05), EW (P < 0.05), and LW (P < 0.001). Jejunum Lactobacillus was positively correlated considerably with VH (P < 0.01), VR (P < 0.05), DW (P < 0.05), EGW (P < 0.01), and LW (P < 0.001). Ileum Lactobacillus was positively correlated with EGW (P < 0.01), EW (P < 0.05), and LW (P < 0.01). Aeriscardovia in duodenum was positively (P < 0.01) associated with EGW. Enterococcus in the duodenum was positively (P < 0.05) associated with EGW and in Jejunum positively correlated with VH (P < 0.05) and VR (P < 0.01). The study concludes that cage rearing improves intestinal health, carcass traits, and microbial diversity in Danzhou chickens, with Lactobacillus and Enterococcus playing key roles.

Suitability of thermally modified wood for binderless particleboard production: Effects of temperature and moisture content in hot pressing

The use of thermally modified wood has grown significantly worldwide over the past 20 years owing to its natural, chemical-free properties, enhanced dimensional stability, and improved biological durability. The binderless particleboard (BP) production process offers an effective way to recycle waste from thermally modified wood. In this study, Fourier-transform infrared (FTIR) spectroscopy and modulated temperature technology were employed to investigate the chemical changes in thermally modified wood as a function of temperature. The impact of moisture content (MC) and temperature on the compression characteristics of particleboards was also examined. The resulting particleboards were subjected to tests for thickness swell and structural morphology (SEM) and vertical density profile analyses. The particleboards produced from thermally modified wood with an MC of 20 % and at a hot-pressing temperature of 170 °C exhibited superior properties to those produced at 8 % MC and 150 and 190 °C. This superior performance can be attributed to the chemical changes in wood, particle size, and the heat transfer and conductivity of the particleboards at high temperature and MC, which renders them more malleable during hot pressing. The particleboards manufactured at 20 % MC and at a hot-pressing temperature of 170 °C demonstrated an internal bonding (IB) of 0.77 MPa, a thickness swell (TS) of 8.14 %, and a homogeneous density distribution. The study provides valuable insights into the production of particleboards from thermally modified wood by optimising the MC and temperature conditions for hot pressing. This research offers a sustainable approach to reusing thermally modified wood waste and promotes the development of more efficient particleboard production processes. Future research could focus on broadening the application scope and exploring the long-term durability and performance of the particleboards produced under the identified optimal conditions.

Exploring synthesis and annealing influence on WS2 (tungsten disulfide): Structural, morphological, optical, and magnetic profiles

The resurgence of interest in Transition Metal Dichalcogenides (TMDCs) stems from their remarkable magnetic and optical properties, reminiscent of graphene. TMDCs like MoS2, MoSe2, WS2, and WSe2 exhibit substantial bandgaps that shift from indirect to direct in single layers. This characteristic enables diverse applications such as solar cells, transistors, photodetectors, and electroluminescent devices, without the direct use of graphene. In this study, polycrystalline WS2 powder was synthesized via the solid-state reaction method. We systematically investigated the structural, morphological, optical, and magnetic properties concerning the synthesized WS2 and its variants annealed at different temperatures. Through X-ray powder diffraction (XRD) analysis, we confirmed the hexagonal structure of the as-synthesized compound, with a space group of P 63/mmc. Interestingly, no structural transitions were observed in the annealed samples. Morphological examination revealed a plate-like structure across all samples. The determination of the optical bandgap was facilitated by UV-DRS absorption spectrum analysis. Our findings showcased the ferromagnetic behavior of the synthesized WS2, corroborated by magnetic measurements and electron paramagnetic resonance (EPR) analysis. These insights contribute to a deeper understanding of TMDCs and pave the way for their tailored utilization in advanced technological applications.

Morphology, Morphometry, and Immunohistochemical Profile of Megakaryocytes and Bone Marrow Microenvironment in Disease Progression and Therapy Resistance in Chronic Myeloid Leukemia.

Background Tyrosine kinase inhibitors have revolutionized the treatment of chronic myeloid leukemia (CML) since the beginning of the century. However, resistance to therapy and the progression of disease tend to occur in certain patients. The bone marrow microenvironment may play a role in the disease outcome. Megakaryocytes have multiple roles in the regulation and maintenance of the hematopoietic stem cell microenvironment. In the current study, we evaluated the association of megakaryocyte morphology, morphometry, and microenvironment with disease progression and therapy resistance in CML. Methodology Megakaryocyte morphology and morphometry were analyzed and compared between the different phases (chronic and advanced) at diagnosis in 150 cases of BCR-ABL-positive CML. All CML-CP patients (n = 119) were followed up on tyrosine kinase inhibitor therapy for a minimum of 15 months and classified based on their treatment outcome as a response, resistance to therapy, or progression of disease based on standard criteria. Immunohistochemistry on a bone marrow trephine biopsy was done for vascular endothelial growth factor (VEGF), FOXP3, CD150, CD48, CD44, osteopontin, CXCL12, N-cadherin, PDL-1, and IL-7, and their expression on megakaryocytes and their association with treatment outcome was evaluated. Results The morphology and morphometry of megakaryocytes showed a heterogeneous population in CML. Morphology and morphometric parameters, when compared between the chronic and advanced phases of disease at diagnosis, did not show any statistical difference. Megakaryocytes were variably positive for VEGF, FOXP3, CD150, CD48, osteopontin, N-cadherin, CXCL12, CD44, PDL-1, and IL-7. However, only CD44-positive megakaryocytes were statistically associated with the treatment outcome. The patients with a higher expression of CD44 megakaryocytes progressed to the advanced phase of the disease during therapy compared to those who responded. Conclusion Megakaryocyte morphology and morphometry were heterogeneous in CML; however, they did not show any significant difference with either the phase of the disease or with treatment outcomes. Among the various immunohistochemical markers of the microenvironment, only CD44-positivity on megakaryocytes was associated with poor treatment outcomes.

Morphological, phylogenetic and metabolite profile of Prorocentrum clipeus, a newly recorded epiphytic dinoflagellate in the northern Yellow Sea

Morphological, phylogenetic and metabolite profile of Prorocentrum clipeus, a newly recorded epiphytic dinoflagellate in the northern Yellow Sea

Body weight and morphological profile of blood of deer (Cervus elaphus) in the conditions of Polissia Volyn

The homeostasis of the whole organism and the analysis of hematological parameters in red deer (Cervus elaphus) play an essential role in comparative physiology for health assessment and disease diagnosis. The obtained blood results can be the basis for assessing the condition of wild deer populations with an existing clinical picture or characterize the nutritional status, habitat, or other stress factors, as the hematopoietic system is susceptible to any changes in the animal body. In the course of the research, it was found that the body weight of adult deer varied depending on the population. Female deer of the Latvian and Lithuanian crossbreeds and the Eastern European population had the highest weight. As for the males, the animals of the Hungarian population had a higher weight than the English population. A high level of growth intensity in the early postnatal period was characteristic of both young females (body weight gain was in the range of 44.8–45.8 kg) and males (42.6–44.12 kg). The analysis of hematological parameters confirmed that the number of red blood cells and hemoglobin content increased in the blood of animals of the English population under technological stress (by 5.6 and 4.4 %). Regarding individual populations, it was noted that under the same conditions of immobilization of animals (medication), the number of red blood cells in the blood of Latvian crossbred deer was 8.5 % higher than the English population. The hemoglobin content was 5.3 % lower, and the saturation of red blood cells with hemoglobin was 12.9 % lower. It was found that the hematological parameters of 3–4 month-old fawns (females and males) of the English population were characterized by relative stability. Against the background of a tendency to a higher number of red blood cells in females (by 12.8 %), the hemoglobin content was 3.3 % lower than in males. The number of leukocytes in the blood of females was slightly higher than that of males, although no significant deviations were noted in some forms of white blood cells.

Parental exposure to acrylamide disrupts sphingolipid metabolism and impairs transgenerational neurodevelopment in zebrafish (Danio rerio) offspring

Acrylamide exposure has become an emerging environmental and food safety issue, and its toxicity poses a potential threat to public health worldwide. However, limited studies have paid attention to the detrimental effects of parental exposure to acrylamide on the neurodevelopment in zebrafish offspring. In this study, the embryos were life-cycle exposed to acrylamide (0.125 and 0.25 mM) for 180 days. Subsequently, these zebrafish (F0) were allowed to mate, and their offspring (F1) were collected to culture in clean water from embryos to adults. We employed developmental and morphological observations, behavioral profiles, metabolomics analyses, and transcriptional level examinations to investigate the transgenerational neurotoxicity with parental exposure to acrylamide. Our results showed that parental exposure to acrylamide harms the birth, development, and behavior characterization of the F1 zebrafish larvae, including poor egg quality, increased mortality rates, abnormal heart rates, slowed swimming activity, and heightened anxiety behavior, and continuously disturbs mental health in F1 adult zebrafish. The transcriptional analysis showed that parental chronic exposure to acrylamide deteriorates the neurodevelopment in F1 larvae. In addition, metabolomics analyses revealed that sphingolipid metabolism disruption may be associated with the observed abnormal development and behavioral response in unexposed F1 offspring. Overall, the present study provides pioneer evidence that acrylamide induces transgenerational neurotoxicity via targeting and disrupting sphingolipid metabolism, which reveals intergenerational transmission of acrylamide exposure and unravels its spatiotemporal toxicological effect on neurodevelopment.

Beneath HMGA2 alterations in pleomorphic adenomas: Pathological, immunohistochemical, and molecular insights

AimsMost salivary gland neoplasms are distinguished by specific recurrent gene fusions. Recently, a subset of pleomorphic adenomas (PAs) originated from the parotid gland harboring the HMGA2:WIF1 fusion was described with a canalicular adenoma-like morphology and a greater propensity for recurrence and carcinomatous transformation. Methods and resultsThis study delineates the clinicopathological attributes of 54 cases of PAs exhibiting HMGA2 alterations, predominantly characterized by the HMGA2:WIF1 fusion, alongside a comparative analysis of their morphological and immunohistochemical profiles. The cohort consisted of 23 females and 31 males (n = 54), mean age was 56.7 (25–84), tumors predominantly originated from the parotid gland (94.4%, 51/54), with 3 cases from seromucous glands (5.6%). Mean tumor size was 2.6 cm (0.8–7.5). No clinical difference (demographic, follow-up) was observed among histological subsets (conventional, hybrid, and pure). Complete excision was performed in all cases, with follow-up data available for 41% (22/54) of patients, showing 13.6% of recurrence (3/22) between 5 and 8 months. Various histological growth patterns were identified, with the pure hypercellular monomorphic subset being the most prevalent. The HMGA2:WIF1 gene was identified in all subsets without any particular predominance. Novel gene partners of HMGA2 were identified, comprising NRXN1, INPP4B, MSRB3, PHLDA1, and FLJ41278. ConclusionsThe present study reports that the HMGA2:WIF1 gene fusion was present in all subsets of PAs without significant predominance. However, further investigations are warranted to explore the relationship between histological subsets of PAs and the molecular alterations underlying them.

Dietary supplementation of Cannabis sativa residues in broiler chickens affects performance, carcass characteristics, intestinal morphology, blood biochemistry profile and oxidative stability

Public interest in the cannabis plant has increased after its legalization in many countries. Cannabis sativa residues (CR) are a part of the plant waste in the cannabis industry. The CR contain medicinal properties that could be used as a feed additive in poultry production. The trial was conducted to investigate the effects of CR on growth performance, carcass characteristics, intestinal morphology, and blood biochemistry profile of broiler chickens. In a completely randomized design, 256 one-day-old male Ross 308 broilers were randomly allocated to 4 treatments with 8 replicates and 8 birds per replicate. These 4 dietary treatments included a basal diet with 0, 0.5, 1 and 2% CR for 40 d. The results showed that 2% CR supplementation reduced feed intake (FI) in the starter phase (d 3-23, P < 0.05). The birds in the CR groups had lower FI in the finishing phase (d 24-40, P < 0.01) and the whole raising period (d 3-40, P < 0.01) than the control. However, the body weight and carcass yield were not different (P > 0.05). In addition, the CR diet had no adverse effects on the blood biochemistry profile, including total cholesterol, triglycerides, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total protein, globulin, albumin, and direct bilirubin (P > 0.05). In addition, total bilirubin and malondialdehyde were better in the plasma of CR-supplemented birds than in the control groups (P < 0.05). The observations on intestinal morphology showed that CR supplementation improved the ratio between villus height and crypt depth in the ileum (P < 0.05). In conclusion, CR supplementation can improve intestinal morphology and oxidative stability of broiler chickens. This suggests that CR could potentially be used as an alternative feed additive in broiler production.