Morphological Patterns Research Articles

Fluctuation-driven morphological patterning: An unconventional approach to morphogenesis

Recent experimental investigations into regeneration revealed a remarkable phenomenon: the morphological transformation of a tissue fragment from the incipient spherical configuration to a tubelike structure—the hallmark of a mature —has the dynamical characteristics of a first-order phase transition, with calcium field fluctuations within the tissue playing an essential role. This morphological transition was shown to be generated by activation over a barrier within an effective morphological potential that underlies morphogenesis. Inspired by this intriguing insight, we propose an unconventional mechanism where stochastic fluctuations drive the emergence of morphological patterns. Thus, the inherent fluctuations determine the nature of the dynamics and are not incidental noise in the background of the otherwise deterministic dynamics. Instead, they play an important role as a driving force that defines the attributes of the pattern formation dynamics and the nature of the transition itself. Here, we present a simple model that captures the essence of this mechanism for morphological pattern formation. Specifically, we consider a one-dimensional tissue arranged as a closed contour embedded in a two-dimensional space, where the local curvature of the contour is coupled to a non-negative scalar field. An effective temperature parameter regulates the strength of the fluctuations in the system. The tissue exhibits fluctuations near a circular shape at sufficiently low coupling strengths, but as the coupling strength exceeds some critical value, the circular state becomes unstable. The nature of the transition to the new state, namely whether it is a first-order-like or second-order-like transition, depends on the effective temperature and the effective cutoff on the wavelength of the spatial variations in the system. The former sets the level of noise, while the latter determines the height of the potential barrier between the initial circular state and the final deformed state of the tissue. It is also found that entropic barriers separate various metastable states of the system. Published by the American Physical Society 2024

Spatial Profiling of Ovarian Clear Cell Carcinoma Reveals Immune-Hot Features

Ovarian clear cell carcinoma (OCCC) has a high incidence in Asia, with a frequent occurrence at an early stage, but without sufficient data on molecular stratification for high-risk patients. Recently, immune-hot features have been proposed as indicators of poor prognosis in early stage OCCC. Specific patterns of intratumoral heterogeneity associated with immune-hot features must be defined. NanoString Digital Spatial Profiling technology (Cold spring biotech corp.) was used to decipher the spatial distribution of the 18-plex protein panel. Regions of interest (ROIs) were collected based on the reference hematoxylin and eosin–stained morphology. Areas of illumination (AOIs) were defined according to the ROI segmentation using the fluorescence signals of the visualization markers pan-cytokeratin (PanCK), CD45, or DNA. Unsupervised hierarchical clustering of 595 AOIs from 407 ROIs showed that the PanCK segments expressed different combinations of immune markers, suggestive of immune mimicry. The following 3 immune-hot clusters were identified: granzyme B–high, immune signal-high , and immune-like cells; the following 2 immune-cold clusters were identified: fibronectin-high and immune checkpoint–high cells. In tumor samples at the International Federation of Gynecology and Obstetrics stage IC1/2 experiencing recurrence, there was an increased occurrence of PanCK+ AOIs with immune signal-high and immune-like cell groups in the papillary morphology surrounded by macrophage lineage tumor-infiltrating immune cells (TIIs). In contrast, for tumor samples at the International Federation of Gynecology and Obstetrics stage IC3/II with recurrence, PanCK+ AOIs were prevalent in the fibronectin-high group, particularly in those with a tubulocystic morphology surrounded by lymphoid lineage non-TIIs. Our study on the spatial profiling of early stage OCCC tumors revealed that the immune mimicry of tumor cells, presence of TIIs, and morphologic patterns were associated with recurrence, which switched during tumor progression.

A mechanical comparison of the translational traction of female-specific and male soccer boots

ABSTRACT The studded outsole of a soccer boot provides additional traction to players to minimise the risk of slipping while performing high-speed manoeuvres. As excessive traction can lead to foot fixation and injury risk, there has been significant research surrounding the influence of stud configuration on the level of traction generated. This previous research, however, has predominately focused on the stud patterns, foot morphology and lower limb loading patterns of male players. As the popularity of women’s soccer increases, the aim of this investigation was to examine the differences in translational traction of female-specific soccer boots and male soccer boots currently available. A custom-built apparatus was used to determine the translational traction on both natural and artificial grass for four different movement directions. It was hypothesised that the female-specific boot in each pair would produce lower levels of translational traction as they are designed to be safer for female players who are more at risk of lower limb fixation injuries compared to males. An independent samples T-test showed that while there were some loading conditions where female boots produced lower translational traction compared to male boots, across all loading scenarios there was no significant difference between male and female boots (p = 0.818), thus the null hypothesis was rejected.

On the aquatic phenology of sympatric crested and marbled newts (genus Triturus)

Abstract The large-bodied newts Triturus cristatus and T. marmoratus possess ranges that overlap in western France where they frequently share breeding ponds. I test the hypothesis that the aquatic phenology differs between them, as associated with a more aquatic and terrestrial life style respectively. Total adult population sizes were determined in spring in 13 experiments by the capture-mark-recapture methodology, followed by equivalent experiments in summer. Population sizes dropped by an order of magnitude on average whereas species frequencies changed in favour of T. cristatus, suggesting that T. marmoratus leaves the breeding site earlier than T. cristatus. Interspecies hybrids behaved like T. marmoratus. Descriptions are provided of other features (in ecology, morphology, demography, and colouration pattern) that support the different, aquatic versus terrestrial life styles of T. cristatus and T. marmoratus.

Urban Habitat Quality Enhancement and Optimization under Ecological Network Constraints

The process of urbanization leads to the rapid expansion of construction land and brings a series of ecological and environmental problems. The ecological network, as a linear landscape element, is of great significance to improve the quality of the regional ecological environment. In this study, the Morphological Spatial Pattern Analysis (MSPA) and the model of Minimum Cumulative Resistance (MCR) were used to construct the ecological corridors in the central city of Fuzhou, and the land use pattern under the constraints of the ecological network was simulated and quantified by the patch-level land use simulation (PLUS) tool with the results of the identification of ecological corridors. Meanwhile, with the help of InVEST habitat quality model, the regional habitat quality under different development scenarios was compared. The results show that (1) 19 ecological sources and 35 ecological corridors were identified; (2) under the constraints of ecological corridors, the area of forested land in the study area in 2027 was increased by 1.57% and the area of built-up land was reduced by 0.55% compared with that in 2022; (3) and under the constraints of ecological corridors, the mean value of habitat quality in Fuzhou City improved by 0.0055 and 0.0254 compared with 2022 and 2027 natural evolution scenarios, respectively. The study provides decision-making assistance for the construction of ecological corridors from the perspective of land use planning.

Incidence of haploidy and triploidy in trophectoderm biopsies of blastocysts derived from normally and abnormally fertilized oocytes.

We aimed to identify the correlation between morphological pronuclear (PN) status and the genetically determined ploidy configuration in preimplantation embryos. A retrospective observational study was conducted on 1982 embryos displaying normal fertilization and 380 embryos showing an atypical PN pattern, tested for aneuploidies and ploidy status via preimplantation genetic testing (PGT) between May 2019 and May 2024. Ploidy prediction was performed using a validated targeted-NGS approach and a proprietary bioinformatic pipeline analyzing SNPs B-allele frequency information. Ploidy results were obtained in relation to the morphological PN pattern and further stratified by mode of PN observation, maternal age, and embryo quality parameters. Abnormal ploidy results in 2PN-derived embryos were 1% (n = 20/1982): 0.8% showed triploidy and 0.2% haploidy. Ploidy results in relation to PN number in atypical fertilization were as follows: 0PN (n = 150/380) associated with 87.3% of diploidy, 8.7% of haploidy, and 4.0% of triploidy; 1PN-derived blastocysts (n = 73/153) were haploid in 47.7% of cases, 6.5% were triploid, and 45.7% diploid; 2.1PN (n = 23/280) and 3PN patterns (n = 54/280) predicted a triploid result in 34.8% and 74.1% of cases, respectively. PN observation with time-lapse increased ploidy status predictivity from 28.3% to 80.4% (p < 0.01) and reduced expected diploid rates to 19.6% (p < 0.01). Diploidy rate was higher for maternal age ≤ 35years and for morphologically high-grade embryos. Morphological PN check can be improved by incorporating ploidy analysis within the conventional PGT workflow. Euploid 2PN-derived embryos can be further selected removing haploids and triploids, and some atypical PN pattern can be better classified.

Construction of Cross-basin Ecological Security Patterns Based on Carbon Sinks and Landscape Connectivity

Artificial water system creation and land use changes have great effects on ecosystems. The construction of cross-basin ecological security patterns based on carbon sinks and landscape connectivity plays a key role in regional ecological environment protection. The linkage area between the Xiang River and the Li River was selected as the research object. Based on the land use data from 2000 to 2020, this study examined the ecological security network of the Xiang-Li connected region using the InVEST model combined with morphological spatial pattern analysis （MSPA） and evaluated the temporal and spatial evolution of carbon storage and ecological security patterns. The results showed that： ① From 2000 to 2020, the land cover types of the Xiang-Li linkage area were mainly forest land and arable land. The changes of land use types were characterized by decreases in arable land, forest land, and grassland and by increases in watersheds and construction land. ② The carbon storage in the Xiang-Li linkage area was characterized by a blocky distribution, and the high and medium areas were dominant. The carbon stock increased slowly from 2000 to 2010 and decreased dramatically from 2010 to 2020, with a cumulative decrease of 18.32×103 t due to the influence of land use changes. ③ The area of ecological sources （five in total） decreased firstly and then increased, whereas the length of ecological corridors （ten in total） increased firstly and then decreased in the Xiang-Li linkage area. Overall, in the process of urbanization, the distribution of the high ecological resistance value in the Xiang-Li linkage area gradually shifted to the northeast with an expansion, whereas the barycenter of the ecological safety pattern shifted to the southwest. Determining the dynamic distribution and stability of ecological sources by coupling carbon storage patches and landscape patterns can provide a new way to construct ecological security patterns in cross-basin ecosystems.

Role of motility and nutrient availability in drying patterns of algal droplets.

Sessile drying droplets in various bio-related systems attracted attention due to the complex interactions between convective flows, droplet pinning, mechanical stress, wettability, and the emergence of unique patterns. This study focuses on the drying dynamics of Chlamydomonas reinhardtii (chlamys), a versatile model algae used in molecular biology and biotechnology. The experimental findings shed light on how motility and nutrient availability influence morphological patterns- a fusion of macroscopic fluid dynamics and microbiology. This paper further discusses the interplay of two competing stressors during drying- nutrient scarcity (quantitative analysis) and mechanical stress (qualitative analysis), where the global mechanical stress does not induce cracks. Interestingly, motile chlamys form clusters under nutrient scarcity due to metabolic stress, indicating the onset of flocculation, a common feature observed in microbial systems. Moreover, non-motile chlamys exhibit an "anomalous coffee-ring effect" in the presence of nutrients, with an inward movement observed near the droplet edge despite sufficient water in the droplet. The quantitative image processing techniques provide fundamental insights into these behaviors in classifying the patterns into four categories (motile+with nutrients, motile+without nutrients, non-motile+with nutrients, and non-motile+without nutrients) across five distinct drying stages- Droplet Deposition, Capillary Flow, Dynamic Droplet Phase, Aggregation Phase, and Dried Morphology.

Variation in air sac morphology and postcranial skeletal pneumatization patterns in the African grey parrot.

The anatomy of the avian lower respiratory system includes a complex interaction between air-filled pulmonary tissues, pulmonary air sacs, and much of the postcranial skeleton. Hypotheses related to the function and phylogenetic provenance of these respiratory structures have been posed based on extensive interspecific descriptions for an array of taxa. By contrast, intraspecific descriptions of anatomical variation for these features are much more limited, particularly for skeletal pneumatization, and are essential to establish a baseline for evaluating interspecific variation. To address this issue, we collected micro-computed tomography (μCT) scans of live and deceased African grey parrots (Psittacus erithacus) to assess variation in the arrangement of the lungs, the air sacs, and their respective invasion of the postcranial skeleton via pneumatic foramina. Analysis reveals that the two pairs of caudalmost air sacs vary in size and arrangement, often exhibiting an asymmetric morphology. Further, locations of the pneumatic foramina are more variable for midline, non-costal skeletal elements when compared to other pneumatized bones. These findings indicate a need to better understand contributing factors to variation in avian postcranial respiratory anatomy that can inform future intraspecific and interspecific comparisons.

Patellar morphology is different in patellofemoral instability: An MRI comparative case-control study

PurposeAlthough patellofemoral instability (PFI) affects both femoral and patellar compartments, literature provided little attention for the patellar morphology contribution on PFI. This study evaluates the patellar morphology patterns on MRI to establish their contribution in PFI. MethodsThis study retrospectively analyzes patellar MRI and X-ray measurements performed between 2018 and 2022. 50 knees with recurrent patellar dislocation were matched with 50 matched knees of ACL-reconstruction candidates with no history of patellar dislocation based on age and gender. Caton-Deschamps’ index, Wiberg’s patellar morphotype, Dejour’s trochlear dysplasia classification, sagittal patellofemoral engagement index and additional patellar cartilage and bone parameters and their relative ratio measurements were assessed in both groups. ResultsStudy patients present differences in patellar morphology; a wider lateral facet (p = 0,019) and a narrower medial facet compared to the control group (p < 0,001). The subchondral patellar crest is medialized compared to the control group (p < 0,001). The cartilaginous crest measurements of the patella were not significantly different in both groups yet PFI group presents a wider Wiberg angle (p < 0,001), thus a flatter patella, compared to the control group. ConclusionThe patella in PFI patients presents a larger lateral facet, a narrower medial facet, a flatter surface and a medialized patellar crest compared with the control group. In PFI, a rather medial patellar crest might predispose towards a greater patellar tilt and destabilize the already compromised patellar-trochlear groove congruence. PFI is a multifactorial disease and both trochlea and patella play a role in its manifestation, thus, literature should address patellar morphotype contribution in patellofemoral instability.Level of Evidence: Level III.

Radially patterned morphogenesis of murine hair follicle placodes ensures robust epithelial budding.

The bending of simple cellular sheets into complex three-dimensional (3D) forms requires developmental patterning cues to specify where deformations occur, but how positional information directs morphological change is poorly understood. Here, we investigate how morphogen signaling and cell fate diversification contribute to the morphogenesis of murine hair placodes, in which collective cell movements transform radially symmetric primordia into bilaterally symmetric tubes. Through live imaging and 3D volumetric reconstructions, we demonstrate that Wnt and Shh establish radial patterns of cell fate, cell morphology, and movement within developing placodes. Cell fate diversity at different radial positions provides unique and essential contributions to placode morphogenesis. Further, we show that downstream of radial patterning, gradients of classical cadherin expression are required for efficient epithelial rearrangements. Given that the transformation of epithelial discs into 3D tubes is a common morphological motif used to shape diverseorgan primordia, mechanisms of radially patterned morphogenesis are likely highly conserved across evolution.

Effective cooling networks: Optimizing corridors for Urban Heat Island mitigation

The detrimental impacts of the Urban Heat Island (UHI) effect are widely recognized in cities globally. Despite the natural cooling capacity of urban cold islands (UCIs), their fragmented state diminishes overall effectiveness. Previous research focused on identifying corridors to connect these isolated UCIs, aiming to enhance cooling networks. However, optimal connection strategies remained elusive. This study introduces a novel framework to address this gap. Utilizing ArcGIS Pro's optimal region connection tools alongside Morphological Spatial Pattern Analysis (MSPA) and ecological parameters, corridors in Ghaemshahr, Iran were meticulously planned and assessed. Through minimum cumulative resistance and gravity models, 63 potential corridors totaling 153 km were identified. Optimization procedures then refined this selection to 27 key corridors spanning 22 km, with 67% measuring less than 0.5 km and strategically positioned near UCIs. This prioritizes adjacency, maximizing corridor protection and construction likelihood. This cost-effective approach fosters stronger connectivity between adjacent UCIs, ultimately linking all UCIs within the region. This innovative methodology provides a holistic solution for mitigating UHI effects, promoting sustainable urban development.

New species of Tricholoma in sections Genuina and Fucata from eastern North America

Six new species of Tricholoma (Fr.) Staude in sections Genuina ( Tricholoma amarissimum, Tricholoma lutescentifolium, Tricholoma pudorinum, Tricholoma sapineum) and Fucata ( Tricholoma griseobrunneum, Tricholoma pallidogriseum) are formally described from North America east of the Rocky Mountains based on their nrITS sequence, morphology, ecology, and distribution patterns. Those species are found in summer or fall in deciduous or coniferous forests. Descriptions of their morphological characters are provided, along with photographs of the basidiomata and their micromorphology. The new species are compared with similar species occurring in North America and Europe. Tricholoma felschii Ovrebo, Hughes &amp; Halling, in section Fucata, is here considered to represent three species, T. felschii s. str., Tricholoma subumbrinum A.H. Sm., and the newly described T. griseobrunneum. Emended descriptions of the first two species are provided to accommodate the new concepts.

Patterns of girdle shape and their correlates in Australian limb-reduced skinks.

The evolution of limb reduction in squamates is a classic example of convergence, but the skeletal morphological patterns associated with it are underexplored. To provide insights on the biomechanical and developmental consequences of transitions to limb reduction, we use geometric morphometrics to examine the morphology of pectoral and pelvic girdles in 90 species of limb-reduced skinks and their fully limbed relatives. Clavicle shapes converge towards an acute anterior bend when forelimbs are lost but hindlimbs are retained-a morphology typical of sand-swimmers. This may either indicate functional adaptations to locomotion in fine substrates, or a developmental consequence of complete limb loss. The shape of limb-bearing elements of both girdles (coracoid and pelvis) instead closely mirrors limb reduction, becoming more simplified as undulation replaces limbed locomotion. Integration between girdles decreases in taxa lacking elements of the forelimbs but not hindlimbs, indicating differential selection on each girdle in response to distinct locomotory strategies. However, this pattern becomes less clear when considering phylogenetic history, perhaps because it is limited to one specific clade (Lerista). We show how the functional demands of locomotion can induce changes at different levels of organismal organization, including both external and internal structures.

Further semantic change of the derogatory sociomorpheme tái in Chinese gender-related Internet neologisms

Internet neologisms provide discursive spaces for grassroots netizens. This study examines a pair of gendered labels gaining popularity in Taiwanese online forums: táinán ‘Tai-man’ and táinǚ ‘Tai-woman’. The quantitative keyword analysis based on a corpus of online data suggests that táinán and táinǚ are pejorative labels. The qualitative discourse analysis of the keywords in context presents gender stereotypes associated with táinán and táinǚ, respectively. Converging evidence for the negative attitudes toward táinán and táinǚ is obtained in a questionnaire survey. The shared morpheme tái inherits localness-related ideologies and interacts with gender ideologies concerning ideal manhood and womanhood, and it has come to index an ideological contrast between favorable foreignness and unfavorable localness. This study contributes to the current literature on Chinese morphological patterns studied in social contexts. Moreover, this study presents evidence that changes in social meanings also follow the dominant trajectories of other semantic changes.

A Linguistic Analysis of two Versatile Verbs "qaťa'a" and "šarf" in the Glorious Qur'an with Reference to Their Realizations in English

This study investigates the different morphological patterns, syntactic structures and meanings of the versatile verbs "قطع" and "صرف ". This study analyses these verbs linguistically, providing a holistic view of their usage and versatility within Qur'anic texts. A qualitative approach is used to analyze data. This study aims to investigate the influence of the context on the morphological forms, the syntactic patterns and different meanings of these verbs. Furthermore, it aims to investigate how the translators transfer the linguistic characteristics of these verbs into English. To achieve the aims of the study, the researcher adopts an eclectic model. Accordingly, two verbs are chosen as the study sample. The main findings of this are as follows: firstly, the verb "قطع" is considered versatile due to its meanings only; it has different meanings with fixed syntactic structure (it is transitive in all the selected' Ayahs). The verb "صرف" is considered versatile in accordance with its meanings and syntactic structures; that is, it has different meanings and syntactic structures. Secondly, all the different meanings are considered polysemous senses of the same selected roots. Thirdly, the context is the most influential factor that makes these verbs versatile. Finally, concerning the renderings of the selected Arabic verbs into the TL, it is concluded that sometimes translators have taken the context into account and have rendered some of these verbs into the equivalently TL. Some other times, they do not render these verbs equally.

Research on the Correlation between the Dynamic Distribution Patterns of Urban Population Density and Land Use Morphology Based on Human–Land Big Data: A Case Study of the Shanghai Central Urban Area

The dynamic distribution of urban population density and the interaction with land use elements involve mutual constraints and guidance. However, in the existing research on the relationship between urban population density and land use, the discussion on the distribution patterns of urban population density typically spans long time periods and uses large spatial units, lacking analysis of the dynamic changes in population density within high granularity land parcels over a day. In studies related to the urban built environment, the complex relationships between different-dimensional land use elements and the dynamic distribution of population density also need further exploration. To address these bottlenecks, this study takes Shanghai’s central urban area as an example. Based on 24 h mobile signaling data on weekdays, weekends, and typical holidays, as well as urban land use data, clustering algorithms are used to summarize patterns of dynamic population density distribution. Pearson correlation analysis is then employed to study the correlation between dynamic population density distribution patterns and different land use elements. The results indicate that various urban land use factors such as locational centrality, functional diversity, transportation accessibility, compactness, and landscape quality have different impacts on the dynamic distribution of population density in spatial units, and the dynamic distribution patterns of population density in different land use types also vary. This research contributes to guiding the optimization of spatial quality and formulating planning and management measures that more effectively match construction intensity with population activity density.

The Role of Spatial Morphology in Forest Landscape Fragmentation: Insights From Planted and Natural Forests of the Chinese Loess Plateau

ABSTRACTThis study aimed to emphasize the key role of spatial morphology of planted and natural forests on landscape fragmentation and to furnish a scientific foundation for the effective assessment of ecological restoration projects of vegetation on the Loess Plateau. The spatial morphological pattern and landscape fragmentation characteristics were analyzed using morphological spatial pattern analysis (MSPA) and forest area density methods. This is the inaugural study to reveal the linear and nonlinear relationships between forest landscape fragmentation and its driving factors using machine learning methods and introducing morphological indicators with two different strategies. The results showed significant differences in the spatial patterns and landscape fragmentation characteristics between planted and natural forests. The spatial patterns of planted and natural forests were found to be dominated by “Core” in terms of area, while “Branch” was more prevalent in terms of number. Compared to natural forests, planted forests were more fragmented. The introduction of the MSPA indicator significantly enhanced the explanatory power and predictive performance of the model despite the disparate contribution rates of the driving factors in planted and natural forests. This study highlights the importance of spatial morphology in understanding forest landscape fragmentation and provides a new combination of analytical techniques to better understand the complexity of forest ecosystems. These provide new insights into forest landscape restoration and sustainable management on the Loess Plateau.

Intraspecific variation in leaf morphology of three widespread woody species along climatic gradients

Abstract The variation and plasticity of leaf morphology plays a pivotal role in the response to environmental changes for plant individuals. Discovering the large-scale pattern of such variation can reveal plants' general adaptive strategies. We analyzed leaf morphology of three widespread woody species in the northern hemisphere using specimen data from the iDigBio and GBIF databases, to investigate the variations in the individual mean traits, in the inter- and intra-individual variability of traits, and in the allometry between traits, along climatic gradients. We found that larger and wider leaves were associated with warmer, wetter and low-sunlight habitats, while smaller but wider leaves are linked to higher wind speed, indicating the response of leaf morphology to multiple climate stresses. The inter-individual variation in leaf area was smaller in colder and windier conditions, suggesting the trait convergence among individuals under environmental filtering, while the intra-individual variation in leaf relative width was smaller in warmer habitats, indicating the similar growth optimum of leaves within one individual in more favorable conditions. Finally, the allometric exponent between leaf length (X-axis) and width (Y-axis) became greater under lower solar radiation and higher wind speed, while the squared correlation coefficient (r2) indicating phenotypic integration showed a decoupling trend under colder conditions, indicating that climate affected the variation tendency of leaf relative width during leaf enlargement. These results reveal the common patterns of leaf morphology responding to climate variation spatially and underscore the necessity to consider inter- and intra-individual variability when examining plant responses to environmental changes.

An Analysis of Lukabaras Borrowed Verbs from English

English as an official language, has come in contact with indigenous languages in Kenya. English has therefore become one of the major donors of loanwords to these indigenous languages, Lukabaras being one of the beneficiaries. This study investigated the morpho-phonological constraints that English loan verbs encounter when adapting into Lukabaras. The research utilized Lukabaras native speakers as respondents to elicit data. A purposive sampling technique was employed to select a sample of 15 English loan verbs, which were then analyzed descriptively based on the tenets of Optimality Theory which states that constraints are universal and are fixed in their ranking and apply to all languages. The loan verbs were transcribed, and the study first analyzed the morphological constraints, followed by the phonological constraints. The findings reveal that English and Lukabaras have different morphological and phonological structures, leading to modifications of English loan verbs to fit the morphological and phonological patterns of Lukabaras. Additionally, the study identifies specific morpho-phonological constraints affecting the adaptation of English loan verbs into Lukabaras, including NOCODA, COMPLEX C, MAX IO, ONSET, DEP IO, IDENT IO, DEP-PREF, DEP-SUFFIX, and ALIGN left edge. Furthermore, faithful constraints are ranked higher than markedness constraints in Lukabaras. The study’s findings contribute to the understanding of morpho-phonological adaptation processes in language contact scenarios. Overall, the study’s findings align with the theoretical framework provided by OT, illustrating how languages manage the integration of foreign elements through a balance of faithfulness and markedness constraints. These findings not only enhance the understanding of loanword adaptation in Lukabaras but also contribute to the broader field of language contact and borrowing, providing a detailed case study of how specific constraints operate in a real-world linguistic context.