Morphological Parameters Research Articles

The effect of the probiotic feed additive "Healthy calf" on the clinical and morphological parameters of the blood of calves with bronchopneumonia

The aim of the study was to establish the degree of influence of the feed additive "Healthy calf" on the normalization of clinical and morphological blood parameters, when it was introduced into the treatment regimen for bronchopneumonia in calves.The production experience was carried out in a farm that was safe for diseases of infectious etiology. The experiment involved 30 black-and-white calves aged 20 to 30 days, of which three groups were formed. The first (control) intact group (n=10) included clinically healthy animals. The second (n=10) and third (n=10) experimental groups included animals with a clinical manifestation of nonspecific catarrhal bronchopneumonia. The animals of the second and third groups were prescribed a treatment regimen based on antibacterial therapy. They were prescribed Streptomycin sulfate as an antimicrobial drug, at a dose of 10.0 mg / kg of body weight twice a day until full recovery. At the same time, during the entire experiment, as an addition to the above treatment regimen, animals of the third group were given oral probiotic supplement "Healthy Calf".Blood samples to determine the values of its main clinical and morphological parameters were taken at the beginning of the experiment, as well as on the seventh and 14th days of its conduct. Blood tests were performed using generally accepted techniques.It has been established that the use of probiotic feed additive "Healthy Calf" in addition to treatment regimens for catarrhal nonspecific bronchopneumonia can reduce the degree of anemia and the intensity of the inflammatory process.

Decreased Nuclear Immunoexpression of ING3 is a Frequent Event in Lip Carcinogenesis.

Evaluate the immunohistochemical expression of the ING3 in actinic cheilitis and squamous cell carcinoma of the lower lip. Forty-five specimens of actinic cheilitis and 48 specimens of squamous cell carcinoma of the lower lip were submitted to immunohistochemical detection of ING3. The protein expression in different cellular sublocations was compared between the two groups, and associations with the clinicopathological variables were analyzed. A significance level of 5% was adopted for all tests. Deaths were significantly more frequent in tumors with a high histopathological risk score (p < 0.05). In actinic cheilitis, significant differences were found in the nucleus-cytoplasmic expression of ING3 and expression restricted to the cytoplasm with binary histopathological grading (p < 0.05). In squamous cell carcinoma of the lower lip, there was no statistically significant difference when comparing ING3 expressions with clinical and morphological parameters (p > 0.05). Nucleo-cytoplasmic ING3 expression was significantly lower in squamous cell carcinoma of the lower lip when compared to actinic cheilitis (p < 0.05) and the expression restricted to the cytoplasm was significantly higher in squamous cell carcinoma of the lower lip (p < 0.05). The results of this study suggest that there is a marked decrease in the nuclear expression of ING3 as malignant progression occurs, indicating an impaired tumor suppressor function of this protein in actinic cheilitis and squamous cell carcinoma of the lower lip.

Влияние экологических условий на анатомо-морфологические показатели листьев Weigela

Abstract. The anatomical features of the leaves are susceptible to environmental factors and, therefore, are very plastic in their development. However, how these indicators differ depending on the influence of environmental factors remains unclear, especially with regard to plants growing in agrocenoses. The purpose of our research is to study the variability of anatomical and morphological parameters of Weigela leaves depending on the growing conditions. We have studied the anatomical structure of Weigela leaves in various ecological growing conditions: in the botanical garden "Tree of Friendship" of the Russian Academy of Sciences and the Subtropical Botanical Garden of Kuban (SBGK), according to a set of anatomical and morphological parameters (leaf blade thickness, development of the palisade and spongy parenchyma, upper and lower epidermis). To do this, the leaves of different ecotypes were studied: those growing in a well-lit (average illumination of 53,000 lux) area of the botanical garden "Tree of Friendship" and under the canopy of trees (illumination of 28,000 lux) in the Subtropical Botanical Garden of Kuban (SBGK, Sochi). Our results showed that the anatomical features of weigela leaves vary in varieties depending on their growing places. It is shown that the highest values (thickness: leaf, palisade and spongy parenchyma, lower and upper epidermis) in various environmental conditions were noted in the variety 'Mont Blanc', and the lowest in 'Eva Ratke'. It is noted that the palisade parenchyma is most developed in the 'Mont Blanc' variety, both in conditions of full illumination (84.7±2.9 microns) and in shading (62.2±2.6 microns). The lowest values were obtained for the ‘Eva Ratke’ variety, while a decrease in the thickness of the front garden fabric was noted in full light conditions (33.7±2.4 microns) and an increase in shade (36.3±3.1 microns). Key words: Weigela, anatomical parameters, leaves, illumination, mesophyll cells, palisade parenchyma, stomata

The effect of stacking sequences on energy absorption in axial crushing of CFRP stanchions for the cargo floor structure of airplanes

One of the remaining challenges for advancing the wide application of composite materials is to further improve the mechanical properties of composites. The fiber/matrix damage phenomenon produced by the composite model under axial loading has been observed through the establishment of a numerical model of composite damage. Axial crush tests have been performed on C-type carbon fiber reinforced polymers (CFRP) stanchions for the cargo floor structure of transport category airplanes using a finite element approach. The failure morphology and crashworthiness parameters of the specimens with different fiber layups have been recorded, and the effects of fiber orientation and angle of entrapment on the crashworthiness of the composites have been investigated. The present study demonstrates that the crashworthiness of composites can be effectively improved by fiber orientation design. Appropriate addition of 45° layer and 90° layer can effectively improve the crashworthiness of C-type CFRP laminates, and the performance of the two-angle and spiral laminates design is improved by 26% and 19%, respectively.

Effect of Biostimulants and Glyphosate on Morphophysiological Parameters of Zea mays (L.) Seedlings Under Controlled Conditions

Maize (Zea mays L.) is the major produced crop in South Africa, but numerous abiotic/biotic stressors threaten its production. Herbicides are mainly in the agricultural sector to minimise crop yield losses caused by weed competition. However, with most weeds becoming resistant to glyphosate, South African farmers have used higher herbicide concentrations than typically recommended. This study was conducted to determine the effect of two biostimulants (brassinosteroids and KELPAK) and glyphosate on the morphophysiological parameters of maize seedlings. Experiments were carried out in the glasshouses of the Department of Soil, Crop, and Climate Sciences at the University of the Free State in Bloemfontein for eight weeks over two seasons, 2017/2018 and 2018/2019. The treatments did not significantly affect all maize morphological parameters except the plant dry mass. Compared to the control, plant dry mass was significantly (p &lt; 0.05) increased by 15.72 g when glyphosate was applied in combination with brassinosteroids during the 2019 growing season. The application of glyphosate, brassinosteroids, and KELPAK differed significantly (p &lt; 0.05) between weeks across the physiological parameters in the two seasons: an irrefutable significant increase was recorded in the rates of transpiration between the weeks. Although significant differences were recorded in the chlorophyll fluorescence, chlorophyll, and carotenoid content, these parameters were similar to the control, especially in the last week of data collection. During the early developmental stage of maize, farmers can administer biostimulants—brassinosteroid (5 g ai ha−1) and KELPAK (5% ai ha−1)—alone and in combination in glyphosate-resistant maize cultivars treated with glyphosate to aid maize seedlings.

Investigating the Heterogeneity Effects of Urban Morphology on Building Energy Consumption from a Spatio-Temporal Perspective Using Old Residential Buildings on a University Campus

The significant increase in building energy consumption poses a major challenge to environmental sustainability. In this process, urban morphology plays a pivotal role in shaping building energy consumption. However, its impact may exhibit latent heterogeneity due to differences in temporal resolution and spatial scales. For urban energy planning and energy consumption modeling, it is crucial to pinpoint when and where urban morphology parameters matter, an overlooked aspect in prior research. This study quantitatively explores this heterogeneity, utilizing a detailed dataset from old residential buildings within a university campus. Spatial lag models were employed for cross-modeling across various temporal and spatial dimensions. The results show that annual and seasonal spatial regression models perform best within a 150 m buffer zone. However, not all significant indicators fall within this range, suggesting that blindly applying the same range to all indicators may lead to inaccurate conclusions. Moreover, significant urban morphology indicators vary in quantity, category, and directionality. The green space ratio exhibits correlations with energy consumption in annual, summer, and winter periods within buffer zones of 150 m, 50~100 m, and 100 m, respectively. It notably displays a negative correlation with annual energy consumption but a positive correlation with winter energy consumption. To address this heterogeneity, this study proposes a three-tiered framework—macro-level project decomposition, establishing a key indicator library, and energy consumption comparisons, facilitating more targeted urban energy model and energy management decisions.

Integrated Morphometric and Hypsometric Analysis of Niragantipalli Micro Watershed Using Remote Sensing and Geographical Information System Techniques

Employing Remote sensing (RS) and geographic information system (GIS) for the morphometric parameters analysis are discovered to be tremendous usefulness in the prioritization of watersheds for soil, water conservation and natural resource management at micro watershed level. The analysis of morphometric parameters plays a crucial role for understanding and managing watersheds. In current study an, attempt was to determine the morphological parameters of Niragantipalli micro-watershed in Chikkaballapura District of Karnataka, India. For detail study, Google earth pro, GPS visualizer conversion toll and Arc GIS were used for preparation of DEM and delineation of the watershed boundary. GIS was used for evaluation of basic, linear, areal and relief aspects of morphometric parameters. The Niragantipalli micro-watershed occupies an area of 632 ha with third order stream (truck order) and dendritic drainage pattern. The mean bifurcation ratio of the micro-watershed is 2.5 it suggests that the drainage system formed on homogeneous rock when the influences of geologic structures on the network of streams were negligible and generate sharp peak flow. With a drainage density of 1.31 km/km2, the drainage network is extremely coarse. The watershed is elongated, as shown by the form factor of 0.27 and elongation ratio of 0.58. The results from the morphometric analysis of the watershed are very beneficial for developing and designing conservation structures of soil and watershed management measures. The hypsometric curves’ structure as well as estimated hypsometric integral results reflects the Niragantipalli Micro Watershed erosional stages. As a result, the study concludes that morphometric and hypsometric analysis findings may be useful to stakeholders participating in catchment development and management projects.

Euclid preparation. LIII. LensMC, weak lensing cosmic shear measurement with forward modelling and Markov Chain Monte Carlo sampling

is a weak lensing shear measurement method developed for and Stage-IV surveys. It is based on forward modelling in order to deal with convolution by a point spread function (PSF) with comparable size to many galaxies, sampling the posterior distribution of galaxy parameters via Markov chain Monte Carlo, and marginalisation over nuisance parameters for each of the 1.5 billion galaxies observed by We quantified the scientific performance through high-fidelity images based on the Flagship simulations and emulation of the VIS images, realistic clustering with a mean surface number density of $ arcmin^ for galaxies, and $ arcmin^ for stars, and a diffraction-limited chromatic PSF with a full width at half maximum of $ $ and spatial variation across the field of view. measured objects with a density of $ arcmin^ in $4500 deg^2 $. The total shear bias was broken down into measurement (our main focus here) and selection effects (which will be addressed in future work). We found measurement multiplicative and additive biases of $m_1=(-3.6 $, $m_2=(-4.3 $, $c_1=(-1.78 $, and $c_2=(0.09 $; a large detection bias with a multiplicative component of $1.2 $ and an additive component of $-3 $; and a measurement PSF leakage of $ $ and $ $. When model bias is suppressed, the obtained measurement biases are close to requirement and largely dominated by undetected faint galaxies ($-5 $). Although significant, model bias will be straightforward to calibrate given its weak sensitivity on galaxy morphology parameters. is publicly available at

Developmental characteristics of cutaneous telocytes in late embryos of the silky fowl.

Telocytes (TCs) have been identified in various animals. However, information on TCs in the embryos is still very limited. In this work, the developing skin of the silky fowl was sampled for TCs identification by histology, immunohistochemistry and transmission electron microscopy. In addition, morphological parameters of cutaneous TCs and their location relationships were measured using a morphometry software - ImageJ (FiJi). At the 12th, 16th and 20th day of incubation, in the embryonic skin, telocyte-like cells (TC-L) were observed in the dermis. TCs were PDGFRα+ at the 12th, 16th and 20th day of incubation, but showed CD34+ only at 20th day of incubation in the embryonic dermis. Ultrastructurally, TCs were observed in the dermis at all late embryonic developmental stages. TCs established the homocellular contacts/plasmalemmal adhesion with each other. TCs established heterocellular contacts with melanocytes at 20th day of incubation in the dermis. In addition, the intracellular microvesicles were present in the cytoplasm of TCs. The extracellular microvesicles/exosomes were in close proximity to the TCs. The results confirmed that the locations, immunophenotypes, structural characteristics and relationships of TCs, and revealed the developmental characteristics of cutaneous TCs in late silky fowl embryos.

Assessing the impact of leachate irrigation on Medicago sativa (alfalfa) growth, enzymatic responses, and heavy metal accumulation.

In light of the increasing water scarcity and the need for sustainable waste management, the use of landfill leachate for irrigation has emerged as both a solution and a concern, posing potential risks to soil health and plant vitality. This study examined the multifaceted impacts of leachate irrigation on the soil characteristics, plant growth, and enzymatic activities of Medicago sativa (M. sativa). By exposing alfalfa to different concentrations of leachate, we assessed the influence on heavy metal accumulation, physiological parameters, and enzyme functions. The physicochemical profile of the leachate indicated that the pH was within acceptable limits, but the chemical oxygen demand (COD), biochemical oxygen demand (BOD5), and concentrations of lead (Pb) and aluminum (Al) exceeded regulatory standards. Morphological parameters exhibited dual effects: stimulation at lower leachate doses and inhibition at higher leachate doses. Our findings show that soil acts as a buffer, reducing heavy metal uptake by plants. Enzymatic activities, including catalase, peroxidase, and succinate dehydrogenase, fluctuated significantly at higher leachate concentrations, indicating stress responses. This research underscores the interplay between leachate irrigation, plant physiology, and soil health, emphasizing sustainable management to optimize plant growth and minimize environmental impacts. It also stresses refining leachate application protocols to preserve soil and ecosystem health.

Study of the varietal behavior of 07 durum wheat varieties (Triticum durum Desf.) in the Oued Righ Valley (ITDAS Station)

Cereal production in the Oued Righ Valley is limited by several factors, including high temperatures and the salinity of irrigation water. These constraints cause significant variations in crop behavior and yield. This study aims to evaluate the performance of seven durum wheat varieties at the El Arfiane demonstration and seed production farm (ITDAS) in the Oued Righ Valley in the south East of Algeria. The evaluation focuses on morphological parameters and yield components To identify the variety or varieties that demonstrate favorable performance and yield, thus encouraging local farmers to cultivate them. The results of this study indicate significant differences in morphological parameters such as plant height, spike length, number of spikes per square meter, and number of grains per spike. However, other measured parameters, such as the number of tillers per plant, thousand-grain weight TGW, grain yield, and harvest index, The methodology employed in this study follows a completely randomized block design with three replications. The experimental design includes a set of experimental units arranged in blocks, with treatments randomly assigned within each block, hence the term "completely randomized block." Each treatment appears only once in each block. The blocks are typically placed contiguously to facilitate observations and task execution. This design is widely used in agronomy as it simplifies task execution and the comparison of treatments. Despite an acceptable thousand-grain weight this year, ranging from 37.06g for variety T4 to 27.6g for variety T1, the yields were low. The highest biological yield was 33.11 quintals per hectare for variety T5, while the lowest yield was 11.39 (T2) quintals per hectare. Several factors, including pigeon attacks and harvest losses, contributed to the reduced real yield.

Carex parva and Carex scabrirostris adopt diverse response strategies to adapt to low-light conditions.

In recent years, the visible light intensity of lawns has significantly decreased due to obstructions caused by urban shading objects. Carex has a competitive advantage over other turfgrass in low-light conditions and extensive management. Therefore, exploring their survival strategy in low-light environments is of great significance. This study focuses on two species of Carex, Carex parva and Carex scabrirostris, and investigates their response to low-light conditions (150 μmol/m2/s) by simulating urban lawn conditions. Biomass allocation characteristics, leaf anatomical features, biochemical parameters, root morphology and photosynthetic parameters were measured. (a) Peroxidase activity, specific leaf area, and relative water content are key factors influencing the photosynthetic capacity of the two Carex species. (b) Under low-light conditions, photosynthetic parameters, leaf physiological indicators, and biomass allocation of the two Carex species were significantly affected (p<0.05). Both Carex species increased their investment in leaf biomass, maintained lateral root growth, and cleared reactive oxygen species to maintain their physiological balance. (c) In the simulated urban low-light environment, neither C. parva nor C. scabrirostris produced dauciform roots. In terms of response strategies, C. scabrirostris is a high-photosynthesis investing species with high productivity under low-light conditions, whereas C. parva exhibits minimal response, indicating a slow investment. C. scabrirostris has greater potential for application in low-light environments compared to C. parva. These results provide a theoretical basis for the cultivation and application of these two Carex species, as well as the expansion of turfgrass germplasm resources.

An Easy-to-Handle Route for Bicomponent Porous Tubes Fabrication as Nerve Guide Conduits

Over the past two decades, the development of nerve guide conduits (NGCs) has gained much attention due to the impellent need to find innovative strategies to take care of damaged or degenerated peripheral nerves in clinical surgery. In this view, significant effort has been spent on the development of high-performance NGCs by different materials and manufacturing approaches. Herein, a highly versatile and easy-to-handle route to process 3D porous tubes made of chitosan and gelatin to be used as a nerve guide conduit were investigated. This allowed us to fabricate highly porous substrates with a porosity that ranged from 94.07 ± 1.04% to 97.23 ± 1.15% and average pore sizes—estimated via X-ray computed tomography (XCT) reconstruction and image analysis—of hundreds of microns and an irregular shape with an aspect ratio that ranged from 0.70 ± 0.19 to 0.80 ± 0.15 as a function of the chitosan/gelatin ratio. More interestingly, the addition of gelatin allowed us to modulate the mechanical properties, which gradually reduced the stiffness—max strength from 0.634 ± 0.015 MPa to 0.367 ± 0.021 MPa—and scaffold toughness—from 46.2 kJ/m3 to 14.0 kJ/m3—as the gelatin content increased. All these data fall into the typical ranges of the morphological and mechanical parameters of currently commercialized NGC products. Preliminary in vitro studies proved the ability of 3D porous tubes to support neuroblastoma cell (SH-SY5Y) adhesion and proliferation. In perspective, the proposed approach could also be easily implemented with the integration of other processing techniques (e.g., electrospinning) for the design of innovative bi-layered systems with an improved cell interface and molecular transport abilities.

Assessing the Correlation Between Retinal Arteriolar Bifurcation Parameters and Coronary Atherosclerosis.

The aim of this study was to examine the relationship between the morphological parameters of retinal arteriolar bifurcations and coronary artery disease (CAD). In this cross-sectional observational study, fundus photography was conducted on 444 participants to capture retinal arteriolar bifurcations. A total of 731 fundus photographs yielded 9625 measurable bifurcations. Analyzed bifurcation parameters included the diameters of the parent vessel (d0), the larger branch (d1), and the smaller branch (d2), as well as the angles (θ1) and (θ2) representing the orientation of each branch in relation to the parent vessel, respectively. Additionally, theoretical optimal angles ( ) and ( ), calculated from the measured parameters, provided a benchmark for ideal bifurcation geometry. The study assessed the variation in these parameters across different levels of coronary atherosclerosis severity. After adjusting for anatomical characteristics including the asymmetry ratio, area ratio, and distance to the optic disc, we observed that patients with severe coronary artery stenosis had significant deviations from the theoretical optimal bifurcation angles, with a decrease in ( ) and an increase in ( ) compared to those with moderate stenosis. The findings suggest a clear alteration in retinal arteriolar bifurcation morphology among patients with severe CAD, which could potentially serve as an indicator of disease severity.

Exploring the density and morphology of coconut structures at two locations: a time-based analysis using computer tomography.

The study aimed to observe the internal structure of coconuts from two locations (coastal and non-coastal) using computed tomography (CT). Seventy-six mature coconuts were collected from Wenchang and Ding'an cities in Hainan Province. These coconuts were scanned four times using CT, with a two-week interval between each scan. CT data were post-processed to reconstruct two-dimensional slices and three-dimensional models. The density and morphological parameters of coconut structures were measured, and the differences in these characteristics between the two groups and the changes over time were analyzed. Time and location had interactive effects on CT values of embryos, solid endosperms and mesocarps, morphological information such as major axis of coconut, thickness of mesocarp, volume of coconut water and height of bud (p<0.05). Planting location and observation time can affect the density and morphology of some coconut structures.

Interface-driven microwave absorption enhancement in Mn-optimized Co2FeAl1-xMnx Heusler alloys

To address the urgent need for efficient and stable electromagnetic wave absorbing materials for electromagnetic interference (EMI) protection, we synthesized Co2FeAl1−xMnx Heusler soft magnetic alloys using a high-energy planetary ball milling method. We comprehensively analyzed their structural characteristics, surface morphology, magnetic properties, and electromagnetic parameters to explore their potential in electromagnetic wave absorption. The results show that incorporating manganese (Mn) significantly changes the alloys' crystal structure, surface morphology, and magnetic properties, greatly enhancing their wave absorption performance. The Mn doping specifically alters the surface morphology, which plays a crucial role in influencing the wave absorption characteristics of the Heusler alloys. When the Mn content increased to 25 %, the sample exhibits superior wave absorption, achieving the maximum reflection loss of −44.83 dB at 13.68 GHz with a thickness of 1.5 mm and an effective absorption bandwidth of 5.52 GHz. With a significant increase in the aspect ratio and noticeable changes in surface morphology, the sample with 75 % Mn content shows exceptional performance with an effective absorption bandwidth of 5.68 GHz, representing the highest effective absorption bandwidth in single-phase Heusler alloys. The Mn doping strategy effectively improves the material's impedance matching by adjusting its permittivity and permeability, facilitating multiple polarization relaxation mechanisms and significantly enhancing the alloys' electromagnetic wave absorption efficiency. Our study provides detailed experimental data for optimizing the electromagnetic wave absorption characteristics of Heusler alloys, verifies their practical application potential, and paves the way for developing new high-performance electromagnetic wave absorbing materials.

The Effect of Radionuclide and Chemical Contamination on Morphological and Anatomical Parameters of Plants

This article presents the results of a study of the influence of radionuclide and chemical pollution on the morphological and anatomical parameters of Calamagróstis epigéjos plants growing in the territory of “Degelen” at the Semipalatinsk Test Site (STS). Quantitative data of morphological and anatomical parameters are given, and the content of radionuclides and toxic elements in samples of plants obtained. Statistical processing of the obtained data was conducted. The results revealed that elevated concentrations of radionuclides 137Cs and 90Sr, and the calculated absorbed dose, do not have effects on plants. Changes in the anatomical parameters of leaves and stems were observed at elevated concentrations of the elements: for leaves—Al, Pb, Sr, U, Ni, Rb, Sm; for stems—Al, Cr, Cd, U, Cu, Be, Ni, Sm, Fe. The mesophyll of the leaves and the epidermis of the stems were the most exposed to toxic elements. The data of the anatomical parameters are recommended to be used as indicative parameters of plants grown in chemically contaminated areas.

Impact of growing conditions on vegetation, development and reproductive success of &lt;i&gt;Orchis purpurea&lt;/i&gt; Huds. in the South-Eastern Crimea

The individual and population parameters of lady orchis — Orchis purpurea Huds. (Orchidaceae) under different growing conditions are compared. The onthogenetic type of cenopopulations, age, efficiency, recovery indices, vitality of individuals, plasticity index are determined. The largest number of plants is noted in forest communities. Demographical and morphological parameters differ nonsignificantly. Slight fluctuations in the vitality of individuals are noted depending on the habitat and the conditions of the growing season. The pollination success of plants in steppe biotopes is much higher than that in forest biotopes: the average fruit set in steppe biotopes was 39.63 %, in forest biotopes — 4.97—5.84 %. However, it did not affect reproductive success, the main indicator of which is the presence of new generations of plants: in all studied cenopopulations, a high proportion of individuals of the pregenerative period was noted. It is established that O. purpurea has a wide ecological amplitude and high adaptability to various conditions. It can grow in different habitats without changing morphological, demographic and ontogenetic parameters.

Effect of Initial Surface Morphology and Laser Parameters on the Laser Polishing of Stainless Steel Manufactured by Laser Powder Bed Fusion.

The topological characteristics of the down-skin surfaces for as-built components by laser powder bed fusion (LPBF) are particularly representative, while the study on the improvement of the surface quality of these surfaces remains largely unexplored. Herein, the laser polishing of LPBF-built components with different inclination angles was systematically investigated with an emphasis on the down-skin surfaces. Our result shows that the topography of the top surface is independent of the inclination angle, and the surface topography of the down-skin surface is dominated by additional angle-dependent surface characteristics. It also indicates that the surface roughness can be reduced sharply when increasing the laser power from 40 W to 60 W, and the reduction slows down when further increasing the laser power while decreasing the scanning speed leads to a progressive improvement of the surface morphology. Moreover, a second-order regression model was established to evaluate the influence of the initial surface morphology and polishing parameters on the polished surface roughness and to achieve surface roughness optimization. Therefore, our established methodology can be readily applied to surface morphology manipulation and process optimization for laser polishing of widely used metals and alloys fabricated by the additive manufacturing process.

Setting a Successful Sorting for Extracellular Vesicle Isolation.

Extracellular vesicles (EVs) released by mesenchymal stromal cells (MSCs) contain a set of microRNAs with regenerative and anti-inflammatory roles. Therefore, purified MSC-EVs are envisioned as a next-generation therapeutic option for a wide array of diseases. In this protocol, we report the strategy for successfully sorting EVs from the supernatant of adipose-derived MSCs (ASCs), often used in orthopedics regenerative medicine applications. First, we described the sample preparation, focusing on EV isolation and labeling steps with carboxyfluorescein succinimidyl ester (CFSE) for fluorescence detection; subsequently, we detailed the sorting process, which constitutes the main part of the protocol. In addition to the rules defined by MISEV 2023 and MIFlowCyt EV guidelines, we applied specific experimental conditions concerning nozzle size, frequency, and sheath pressure. Morphological parameters are established using beads of diameters selected to cover the theoretical range of EV size. After ASC-EVs sorting, we performed a purity check of the sorted fraction by re-analyzing it with the sorter and verifying the EV size distribution with the nanoparticle tracking analysis technique. Due to the increasing importance of EVs, having a pure population to study and characterize is becoming crucial. Here, we demonstrate a winner strategy to set up sorting to achieve this goal.