Morphological Development Research Articles

Tracking gonadal development in fish: An in vivo MRI study on polar cod, Boreogadus saida (Lepechin, 1774).

Magnetic resonance imaging (MRI) was applied to determine the sex of polar cod (Boreogadus saida Lepechin, 1774) (Actinopterygii: Gadidae) and to follow the gonadal development in individual animals over time. Individual unanaesthetised fish were transferred to a measurement chamber inside a preclinical 9.4T MRI scanner and continuously perfused with aerated seawater. A screening procedure at an average of 3.5h, consisting of a set of MRI scans of different orientations, was repeated every 4 weeks on the same set of reproducing B.saida (n= 10) with a body length of about 20 cm. Adapted multi-slice flow-compensated fast low-angle shot (FcFLASH) and rapid acquisition with relaxation enhancement (RARE) protocols with an in-plane resolution of 313 μm and an acquisition time of 2.5min were used to visualise the morphology of various organs, including the gonads within the field of view (FOV). The MR images provided high resolution, enabling specific sex determination, calculation of gonad volumes, and determination of oocyte sizes. Gonad maturation was followed over 4 months from November 2021 until shortly before spawning in February 2022. The gonad volume increased by 2.3-25.5% for males and by 11.5-760.7% for females during the observation period. From October to February, the oocyte diameter increased from 427 μm (n= 1) to 1346 ± 27 μm (n= 4). Interestingly, individual oocytes showed changes in MR contrast over time that can be attributed to the morphological development of the oocytes. The results fit well with previous literature data from classical invasive studies. The presented approach has great potential for various ecophysiological applications such as monitoring natural or delayed development of internal organs or sex determination under different environmental conditions.

Biomass of Eichhornia crassipes as an Alternative Substrate for the Formation of Lettuce Seedlings

The production of lettuce has increased significantly due to the use of hydroponic systems that rely on substrates. Disposal and acquisition costs present problems, necessitating the identification of sustainable alternatives. The present study aimed to evaluate the use of Eichhornia crassipes (water hyacinth) dry matter in a substrate for the formation of lettuce seedlings. Water plants were collected to obtain their dry matter, and twelve mixtures were formed with Sphagnum and perlite. Mixtures with more water hyacinth dry matter exhibited greater water retention. However, these mixtures also lost water at a faster rate than those containing primarily Sphagnum dry matter did. Higher percentages of germination were detected in the mixtures with water hyacinth dry matter, but these seedlings also presented higher concentrations of proline, such as 16.0 µg mL−1. The mixtures with water hyacinth dry matter presented the highest ion concentrations, mainly at high levels of humidity. Mixtures with a high proportion of water hyacinth dry matter had a greater water retention capacity and a high percentage of lettuce seed that germinated. The mixtures with a higher proportion of Sphagnum led to greater root length, greater concentrations of chlorophyll in cotyledonary leaves, and better morphological development of the seedlings.

Non-surgical endodontic treatment of a type III dens invaginatus with large perirradicular radiolucency using bioceramic materials: Case report

Dens invaginatus (DI), also known as dens in dente, is an uncommon dental anomaly in humans, characterized by irregular morphological development during odontogenesis, which can manifest in various coronal and radicular anatomies. Endodontic treatment of teeth with this variation demands anatomical knowledge, detailed planning, and the use of specialized techniques and materials. The objective of this research is to detail, through a case report, the non-surgical endodontic treatment of type III dens invaginatus in the upper lateral incisor with extensive periapical lesion, using bioceramic materials. An 18-year-old female patient was referred to the referral center by an orthodontist after radiographic examination revealed an extensive periapical lesion on tooth 12. Consequently, cone beam computed tomography was requested to assess the lesion and internal/external anatomy. The endodontic treatment was performed entirely under operative microscopy, utilizing mechanized instrumentation and thermoplasticized obturation. Disinfection was enhanced through activated irrigation technique and intracanal placement of bioceramic medication, with periapical repair achieved using bioceramic sealer cement. Long-term follow-up demonstrated that conservative endodontic treatment, employing modern techniques alongside bioceramic materials, proved effective in resolving complex cases.

The Association Between the Development of Cam Morphology During Skeletal Growth in High-Impact Athletes and the Presence of Cartilage Loss and Labral Damage in Adulthood: A Prospective Cohort Study With a 12-Year Follow-up

Background: Cam morphology develops during skeletal growth, but its influence on cartilage and the labrum in high-impact athletes later in life is unknown. Purpose: To (1) explore the association between the presence and duration of cam morphology during adolescence and the cartilage and labral status 7 to 12 years later and (2) report the prevalence of cartilage loss and labral damage in a population of young male athletes (<32 years old) who played professional soccer during skeletal growth. Study Design: Cohort study (Prognosis); Level of evidence, 2. Methods: A total of 89 healthy male academy soccer players from the Dutch soccer club Feyenoord (aged 12-19 years) were included at baseline. At baseline and 2.5- and 5-year follow-ups, standardized supine anteroposterior pelvis and frog-leg lateral radiographs of each hip were obtained. At 12-year follow-up, magnetic resonance imaging of both hips was performed. Cam morphology was defined by a validated alpha angle ≥60° on radiographs at baseline or 2.5- or 5-year follow-up when the growth plates were closed. Hips with the presence of cam morphology at baseline or at 2.5-year follow-up were classified as having a “longer duration” of cam morphology. Hips with cam morphology only present since 5-year follow-up were classified as having a “shorter duration” of cam morphology. At 12-year follow-up, cartilage loss and labral abnormalities were assessed semiquantitatively. Associations were estimated using logistic regression, adjusted for age and body mass index. Results: Overall, 35 patients (70 hips) with a mean age of 28.0 ± 2.0 years and mean body mass index of 24.1 ± 1.8 participated at 12-year follow-up. Cam morphology was present in 56 of 70 hips (80%). The prevalence of cartilage loss was 52% in hips with cam morphology and 21% in hips without cam morphology (adjusted odds ratio, 4.52 [95% CI, 1.16-17.61]; P = .03). A labral abnormality was present in 77% of hips with cam morphology and in 64% of hips without cam morphology (adjusted odds ratio, 1.99 [95% CI, 0.59-6.73]; P = .27). The duration of cam morphology did not influence these associations. Conclusion: The development of cam morphology during skeletal growth was associated with future magnetic resonance imaging findings consistent with cartilage loss in young adults but not with labral abnormalities.

A feather star is born: embryonic development and nervous system organization in the crinoid Antedon mediterranea.

Crinoids belong to the Echinodermata, marine invertebrates with a highly derived adult pentaradial body plan. As the sister group to all other extant echinoderms, crinoids occupy a key phylogenetic position to explore the evolutionary history of the whole phylum. However, their development remains understudied compared with that of other echinoderms. Therefore, the aim here was to establish the Mediterranean feather star (Antedon mediterranea) as an experimental system for developmental biology. We first set up a method for culturing embryos in vitro and defined a standardized staging system for this species. We then optimized protocols to characterize the morphological and molecular development of the main structures of the feather star body plan. Focusing on the nervous system, we showed that the larval apical organ includes serotonergic, GABAergic and glutamatergic neurons, which develop within a conserved anterior molecular signature. We described the composition of the early post-metamorphic nervous system and revealed that it has an anterior signature. These results further our knowledge on crinoid development and provide new techniques to investigate feather star embryogenesis. This will pave the way for the inclusion of crinoids in comparative studies addressing the origin of the echinoderm body plan and the evolutionary diversification of deuterostomes.

Myostatin's marvels: From muscle regulator to diverse implications in health and disease.

Myostatin, a member of the transforming growth factor-β superfamily, is a pivotal regulator of skeletal muscle growth in mammals. Its discovery has sparked significant interest due to its multifaceted roles in various physiological processes and its potential therapeutic implications. This review explores the diverse functions of myostatin in skeletal muscle development, maintenanceand pathology. We delve into its regulatory mechanisms, including its interaction with other signalling pathways and its modulation by various factors such as microRNAs and mechanical loading. Furthermore, we discuss the therapeutic strategies aimed at targeting myostatin for the treatment of muscle-related disorders, including cachexia, muscular dystrophyand heart failure. Additionally, we examine the impact of myostatin deficiency on craniofacial morphology and bone development, shedding light on its broader implications beyond muscle biology. Through a comprehensive analysis of the literature, this review underscores the importance of further research into myostatin's intricate roles and therapeutic potential in human health and disease.

Spatiotemporal Characterization of the Three-Dimensional Morphology of Urban Buildings Based on Moran’s I

The three-dimensional morphological analysis of urban buildings constitutes a pivotal component of urban planning and sustainable development. Nevertheless, the majority of current research is two-dimensional in nature, which constrains the comprehensive understanding of urban spatial–temporal evolution. The existing body of three-dimensional studies frequently fails to consider the temporal dimension of architectural change and lacks a detailed examination of micro areas such as communities and streets. In order to accurately identify the patterns of spatial–temporal evolution in urban architectural morphology, this study focuses on the Yau Tsim Mong District in Hong Kong, utilizing three-dimensional data. By innovatively integrating temporal factors, constructing a spatial–temporal weight matrix, and applying the spatial–temporal Moran’s I, this study conducts an in-depth quantitative analysis of Coverage, Staggeredness, and Duty Cycle at the community scale, neighborhood scale, and urban scale. From 2014 to 2023, the global spatial–temporal Moran’s I of key urban morphology indicators in Yau Tsim Mong District has exhibited a marked increase, underscoring the close interrelationship and significant optimization between urban morphology and overall development. The findings illustrate that urban architecture is undergoing a process of agglomeration and high homogeneity, with strategic shifts emphasizing efficient spatial utilization and refined design. The analysis at the neighborhood scale is of particular importance, as its independent and complete spatial structure effectively captures local dynamics, revealing high-value agglomeration and low-value dispersion characteristics. This suggests that buildings in the Yau Tsim Mong District are being constructed in a more compact manner at the neighborhood level, which reflects the precision and efficiency of urban planning and the rationality of spatial planning. These significant findings provide valuable references for the development planning and governance of sustainable cities. They enhance urban governance capabilities and promote the optimization of urban development strategies, ensuring steady progress on the path of efficiency, harmony, and sustainability.

Prenatal assessment of brain malformations on neuroimaging: an expert panel review.

Brain malformations represent a heterogeneous group of abnormalities of neural morphogenesis, often associated with aberrations of neuronal connectivity and brain volume. Prenatal detection of brain malformations requires a clear understanding of embryology and developmental morphology through the various stages of gestation. This expert panel review is written with the central aim of providing an easy-to-understand roadmap to improve prenatal detection and characterization of structural malformations based on the current understanding of normal and aberrant brain development. The utility of each available neuroimaging modality including prenatal multiplanar neurosonography, anatomical magnetic resonance imaging (MRI), and advanced MRI techniques, as well as further insights from post-mortem imaging have been highlighted for every developmental stage.

Isogenic patient-derived organoids reveal early neurodevelopmental defects in spinal muscular atrophy initiation

Whether neurodevelopmental defects underlie postnatal neuronal death in neurodegeneration is an intriguing hypothesis only recently explored. Here, we focus on spinal muscular atrophy (SMA), a neuromuscular disorder caused by reduced survival of motor neuron (SMN) protein levels leading to spinal motor neuron (MN) loss and muscle wasting. Using the first isogenic patient-derived induced pluripotent stem cell (iPSC) model and a spinal cord organoid (SCO) system, we show that SMA SCOs exhibit abnormal morphological development, reduced expression of early neural progenitor markers, and accelerated expression of MN progenitor and MN markers. Longitudinal single-cell RNA sequencing reveals marked defects in neural stem cell specification and fewer MNs, favoring mesodermal progenitors and muscle cells, a bias also seen in early SMA mouse embryos. Surprisingly, SMN2-to-SMN1 conversion does not fully reverse these developmental abnormalities. These suggest that early neurodevelopmental defects may underlie later MN degeneration, indicating that postnatal SMN-increasing interventions might not completely amend SMA pathology in all patients.

Development of derivational morphology in Kuwaiti Arabic-speaking children

The objective of this study was to examine the development of derivational morphological structures in the productive language of Kuwaiti Arabic (KA)-speaking children. Participants were 512 typically developing Kuwaiti children aged 3;0 years to 7;11 years (243 boys and 269 girls). Five age groups at 1-year intervals were tested; each group was divided into four sub-groups at 3-month intervals. The children were examined using a tool designed to assess structures specific to the KA dialect, and they were assessed by trained research assistants at their nurseries and public schools. There were clear developmental trajectories in the derivational morphological structures studied in KA-speaking children across the five age groups. Results showed that KA-speaking children have not reached mastery level in most of the structures examined in this study. Among the two types of derivations examined, nominal derivations emerged later than verb derivations. Error analysis showed that most errors were morphological, affecting mainly the root. The results of this study enhance our knowledge of early language development in Arabic by showing that nominal derivations develop later than verb derivations. The results are discussed in light of previous cross-linguistic studies, which also found a similar trajectory in terms of morphological development. Further, Arabic morphological theory, as well as implications for practice and the need for further research, is discussed.

Periconceptional maternal supplement intake and human embryonic growth, development, and birth outcomes: the Rotterdam Periconception Cohort.

Is periconceptional multiple-micronutrient supplement (MMS) use including folic acid (FA) compared to FA use only associated with increased embryonic growth, development, and birth weight in a high-risk population? Women with MMS intake show no significant differences in first-trimester morphological embryo development, but increased first-trimester embryonic growth trajectories and fewer neonates born small for gestational age (SGA), less than the 3rd percentile (<p3), compared to women using only FA. Periconceptional maternal FA intake in the general population is associated with increased embryonic and fetal growth, and reduced risks of neural tube defects, other congenital malformations, low birth weight, and neonates born SGA. A prospective tertiary hospital-based cohort study (the Rotterdam Periconceptional Cohort) was conducted from January 2010 to December 2020. We included 1076 women from the Rotterdam Periconceptional Cohort, before 10 weeks of pregnancy with follow-up until delivery. Embryonic growth was assessed by measurement of crown-rump length (CRL) and embryonic volume (EV), and embryonic morphology was described by Carnegie stages using longitudinal three-dimensional ultrasound scans and virtual reality techniques. Birth outcomes were extracted from medical records. General characteristics and supplement use were extracted from research questionnaires. This study showed increased embryonic growth trajectories (adjusted models, CRL: β = 0.052, 95% CI 0.012-0.090, EV: β = 0.022, 95% CI 0.002-0.042) in women using MMS compared to those using only FA. Moreover, a 45% reduced risk of a neonate-born SGA (<p3) was shown in women using MMS compared to FA users (adjusted OR = 0.546, 95% CI 0.308, 0.969). Embryonic morphological development (Carnegie stages) and the occurrence of miscarriages did not differ between women using MMS or solely FA. Following the heterogeneity of the composition and dose of MMS preparations, it is unclear which specific micronutrient, combination, or dose explains the increased embryonic growth trajectory and reduction in risk for SGA. This also hampers the possibility of differentiating between the effects of FA alone or as a component of MMS. Our findings emphasize the importance of periconceptional maternal MMS use as a potential preventative intervention against reduced embryonic growth and neonates born SGA. Therefore, we recommend the periconceptional use of MMS in women at risk of inadequate micronutrient intake. However, awareness of potentially harmful side effects of high doses and combinations of micronutrients is essential, therefore the optimal composition and dose need to be investigated, and careful surveillance is recommended. This research was funded by the Department of Obstetrics and Gynaecology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands, and the ZonMw grant Open Competition 2018 (09120011910046). The authors declare that they have no conflict of interest. NTR4356.

On the mining vocabulary functioning in Perm 18th c. written records

Based on the Perm written monuments from the mid- and late 18th c., this article traces the dynamics of changes in the lexical composition of Perm business documents, studies the phenomenon of parallel use of borrowed and original terms in monuments, and describes the previously unrecorded vocabulary. The author analyzes extralinguistic reasons for the appearance of doublet names, as well as the process of partial displacement of borrowed words by their original analogues. Special attention is paid to the degree of morphological and word-formation development of German borrowing terms within the framework of mining vocabulary. The article attempts to clarify the picture of the functioning and the correlation between the original and borrowed mining industry vocabulary. In comparison with the 18th–19th c. Pyskor written records, the author tracks the usage dynamics of the corresponding layers of vocabulary using a previously unexplored writtеn record, Stolp prikhodnoy… 1741 goda [Column of the Incoming ... 1741] and traditional methods of linguistic source studies. The results include establishing parallel names types (borrowed/native term); introducing new lexemes into scientific circulation; new evidence of decreased rate of borrowing and changing some of the borrowed vocabulary with native names in the 18th c.

Development and cross-sectional morphology of the recurrent laryngeal nerves in human fetuses.

The recurrent laryngeal nerve is a bilateral branch of the vagus nerve that is mainly associated with the motor innervation of the intrinsic muscles of the larynx. Despite its bilateral distribution, the right and left recurrent laryngeal nerves display unequal length due to embryological processes related to the development of the aortic arches. This length asymmetry leads to theories about morphological compensations to provide symmetrical functions to the intrinsic muscles of the larynx. In this study we investigated the developmental and cross-sectional morphometrics of the recurrent laryngeal nerves in human fetuses. Fifteen stillbirth fetuses donated to anatomical and medical research were used for investigation. Fetuses had intrauterine age ranging from 30 to 40 weeks estimated by biometry methods. Specialized anatomical dissection of the visceral block of the neck was performed to prepare histological samples of the recurrent laryngeal nerves in its point of contact with the larynx, and morpho-quantitative techniques were applied to evaluate the epineurium and perineural space of the recurrent laryngeal nerves. No statistical difference in the cross-sectional morphology of the epineurium and perineural space between right and left recurrent laryngeal nerves intra-individually was confirmed, however, we found evidence that these structures are under greater development in the left recurrent laryngeal nerve during 30 to 40 weeks of intrauterine life. Our data suggest that the nerves are under morphological development that possibly set the stage for accommodation of larger diameter and myelinization of the left recurrent laryngeal nerve during post-natal life.

No sex difference in maturation of brain morphology during the perinatal period.

Accumulating evidence have documented sex differences in brain anatomy from early childhood to late adulthood. However, whether sex difference of brain structure emerges in the neonatal brain and how sex modulates the development of cortical morphology during the perinatal stage remains unclear. Here, we utilized T2-weighted MRI from the Developing Human Connectome Project (dHCP) database, consisting of 41 male and 40 female neonates born between 35 and 43 postmenstrual weeks (PMW). Neonates of each sex were arranged in a continuous ascending order of age to capture the progressive changes in cortical thickness and curvature throughout the developmental continuum. The maturational covariance network (MCN) was defined as the coupled developmental fluctuations of morphology measures between cortical regions. We constructed MCNs based on the two features, respectively, to illustrate their developmental interdependencies, and then compared the network topology between sexes. Our results showed that cortical structural development exhibited a localized pattern in both males and females, with no significant sex differences in the developmental trajectory of cortical morphology, overall organization, nodal importance, and modular structure of the MCN. Furthermore, by merging male and female neonates into a unified cohort, we identified evident dependencies influences in structural development between different brain modules using the Granger causality analysis (GCA), emanating from high-order regions toward primary cortices. Our findings demonstrate that the maturational pattern of cortical morphology may not differ between sexes during the perinatal period, and provide evidence for the developmental causality among cortical structures in perinatal brains.

Formation mechanism and regulation analysis of trumpet leaf in Ginkgo biloba L.

The research on plant leaf morphology is of great significance for understanding the development and evolution of plant organ morphology. As a relict plant, the G. biloba leaf morphology typically exhibits bifoliate and peltate forms. However, throughout its long evolutionary history, Ginkgo leaves have undergone diverse changes. This study focuses on the distinct "trumpet" leaves and normal fan-shaped leaves of G. biloba for analysis of their phenotypes, photosynthetic activity, anatomical observations, as well as transcriptomic and metabolomic analyses. The results showed that trumpet-shaped G. biloba leaves have fewer cells, significant morphological differences between dorsal and abaxial epidermal cells, leading to a significantly lower net photosynthetic rate. Additionally, this study found that endogenous plant hormones such as GA, auxin, and JA as well as metabolites such as flavonoids and phenolic acids play roles in the formation of trumpet-shaped G. biloba leaves. Moreover, the experiments revealed the regulatory mechanisms of various key biological processes and gene expressions in the trumpet-shaped leaves of G. biloba. Differences in the dorsal and abdominal cells of G. biloba leaves can cause the leaf to curl, thus reducing the overall photosynthetic efficiency of the leaves. However, the morphology of plant leaves is determined during the primordia leaf stage. In the early stages of leaf development, the shoot apical meristem (SAM) determines the developmental morphology of dicotyledonous plant leaves. This process involves the activity of multiple gene families and small RNAs. The establishment of leaf morphology is complexly regulated by various endogenous hormones, including the effect of auxin on cell walls. Additionally, changes in intracellular ion concentrations, such as fluctuations in Ca2+ concentration, also affect cell wall rigidity, thereby influencing leaf growth morphology.

Morphological and functional spleen development in crossbreed rabbits

The accelerated growth of muscle mass in productive broiler breeds is often associated with delayed organ development of integral body systems, particularly the immune structures. The spleen is the largest secondary immune organ in the mammalian body and is responsible for initiating immune responses to blood-borne antigens. It can only perform this function successfully if all of its tissue components are fully morphologically and functionally mature. The spleen was studied in meat production rabbits (early maturing crossbreed Hyplus) at 1, 10, 20, 30, 60 and 90 days of age. Morphological studies included anatomical dissection, clarification of topography, determination of mass parameters, preparation of smears and histological sections. Spleen histological sections were stained with haematoxylin and eosin, according to Van Gieson, and impregnated with silver nitrate, followed by microscopy. Qualitative and quantitative indicators of spleen cellular and tissue component development were determined using ImageJ software. It was found that in crossbred rabbits the topography of the spleen corresponds to general anatomical principles of localisation, has a fixed place and is an anatomically formed organ. In day-old animals, the histological differentiation of the spleen is limited to the connective tissue stroma and the parenchyma. The organ's parenchyma is formed by reticular tissue, with hematopoietic and lymphoid cells among the cells, without differentiation into white and red pulp. In 10-day-old rabbits, the white pulp is represented by the periarterial lymphatic sheath (PALS) and marginal zones. Single primary lymphoid nodules without germinal centres are seen in the spleen from 20 days of age. By 30 days of age, the white splenic pulp has all the major structural and functional zones, including formed lymphoid nodules with germinal centres and mantle zones. During the second and third months of life, the spleen gradually increases the relative area of all white pulp functional zones, reaching a maximum at 90 days of age. In productive rabbits, the cellular composition of the splenic white pulp is represented by lymphocytes (small, medium, large), reticular cells, macrophages and plasma cells. During postnatal ontogenesis, the number of small lymphocytes increases in all white pulp functional zones, reaching maximum values in 90-day-old animals. On the contrary, the relative number of medium and large lymphocytes decreases. The number of reticulocytes in the periarterial lymphatic sheath and lymphoid nodules zones does not change significantly, and in the marginal zone it decreases substantially by the end of the study. The results of determining the timing of morphological and functional maturation of immunocompetent structures in the spleen of meat rabbits are of great biomedical and economic importance. They will serve as a control for comparing changes in rabbit spleen lymphoid tissues during the development of pathological processes, as well as under the influence of external factors.

Morphological development of drying shrinkage cracks at the rock[sbnd]soil interface in a karst rocky desertification area

Study regionkarst rocky desertification area, China Study focusThe rocksoil interface, where exposed bedrock is in abrupt contact with the soil, is prevalent in rocky desertification areas. As a constraining boundary condition of soils, drying shrinkage cracks are easily developed at the rocksoil interface of outcrops with weak or un-weathered bedrock, but the shrinkage crack development under these conditions is still unknown, which seriously restricts a profound understanding of the hydrological processes on karst slopes with exposed bedrocks. This study aims to quantify the geometric morphological characteristics of these shrinkage cracks and the block areas cut by the crack network during the crack development, as well as to explore the development of drying shrinkage cracks at the rocksoil interface of outcrops. New hydrological insightsWhen the soil moisture content was between 24.1 % and 28.6 %, shrinkage cracks at the rocksoil interface (CRSI) first formed along the border between rocks and soils and then at the soil surface perpendicular to the former, most of the cracks intersected vertically in shapes of "T" or "+". The geometric parameters of shrinkage cracks initially increased before stabilizing and reached a stabilization stage as soon as the moisture content decreased. However, all of them finally shrank slightly in the later stages of soil water loss in thicker soils. Soil thickness was not a crucial factor affecting the CRSI formation. The CRSI were 1.2 times wider than all other cracks, while being infrequent and making up just 40 % of the total length and area of all cracks. As a result, the CRSI may grow into a key pathway for the establishment of preferred flow at the rocksoil interface, which should be paid attention in the hydrological processes in the karst rocky desertification region.

Life and death of a thin liquid film

Thin films, bubbles and membranes are central to numerous natural and engineering processes, i.e., in solar cells, coatings, biosensors, foams, and emulsions. Yet, the characterization and understanding of their rupture is limited by the scarcity of atomic detail. We present here the complete life-cycle of freely suspended films using non-equilibrium molecular dynamics simulations of a simple atomic fluid free of surfactants and surface impurities, thus isolating the fundamental rupture mechanisms. We identified a short-term ‘memory’ by rewinding in time from a rupture event, extracting deterministic behaviors from apparent stochasticity. A comprehensive investigation of the key rupture-stages including both unrestrained and frustrated propagation is made—characterization of the latter leads to a first-order correction to the classical film-retraction theory. The highly resolved time window reveals that the different modes of the morphological development, typically characterized as nucleation and spinodal rupture, continuously evolve seamlessly with time from one into the other.

Development of CaCO3 novel morphology through crystal lattice modification assisted by sulfate incorporation and vibration

CaCO3 has long been used as a filler to increase many properties of the material. The filler commonly consists of inexpensive materials that replace some volume of the more expensive materials, which can reduce the cost of the final product. CaCO3 morphology that can be used as filler depends on the filler's function, such as filler for paper, paint, rubber, or composite. A filler for composite materials is needed to increase interfacing interactions between the particulate fillers and the matrix. So, the particulate in a broader shape will be the best choice to function for such filler. In this research, in an attempt to increase the interfacing interaction, CaCO3 morphology was modified in such a way through crystal lattice modification assisted by sulfate incorporation and vibration. SEM analysis was implemented, and showed that the research successfully produced novel morphology in branchy-like polymorphs. FTIR analysis also proved that the crystal lattice has been modified. The morphology in branchy-like polymorph is supposed to increase interfacing interaction between CaCO3 as the filler and the matrix. The methods are also supposed to be implemented as the research is scaled up to commercial scale.

Molecular Mechanisms of MADS-box Genes in Regulating Floral Organ Development in Rice

As one of the most important food crops in China and worldwide, the morphological development of floral organs in rice is crucial, directly affecting its yield and quality. The MADS-box gene family significantly influences the development of floral organs in rice (Oryza sativa L.). This paper reviews the ABCDE model of floral organ development and provides an overview of recent advancements in research on MADS-box genes that influence the characteristics of floral organs in rice.