Morphological Affinities Research Articles

Description of a new species of potter wasp (Hymenoptera, Vespidae, Eumeninae) from Northeast India

Pseumenes Giordani Soika, 1935 is a small genus of potter wasps occurring in Oriental, Australian and Palearctic Regions. Only one species, Pseumenes depressus (de Saussure, 1855) is known so far from India. A new species, Pseumenes siangensis sp. nov. from Arunachal Pradesh, is described. The morphological affinities of the new species are discussed. The new species is compared with the closely related P. depressus as well as P. laboriosus. Since P. depressus is similar to P. laboriosus, comparisons were made between P. laboriosus and P. siangensis sp. nov. The apical teeth of the propodeum are medium sized and blunt in P. siangensis sp. nov. (long and sharp in P. laboriosus); the posterior part of the first tergite is densely punctate in the middle P. siangensis sp. nov. (almost impunctate in P. laboriosus). The clypeus without a median black spot in P. siangensis sp. nov. (with median black spot in P. laboriosus).

A new species of Platypterygius (Ophthalmosauridae) from the lower Barremian of Colombia and assessment of the species composition of the genus

In this paper, we describe a new ophthalmosaurid specimen found in the lower Barremian beds of the Paja Formation at Villa de Leiva, Boyacá, Colombia. The specimen represents a new species of Platypterygius, P. elsuntuoso sp. nov. defined by three unique features within the genus: a small ventral peg in the anterior ventral border of the extracondylar area of the basioccipital; a supratemporal groove in the quadrate; and a shallow neck at the base of the acellular cementum ring on the teeth root. In support of our taxonomic determination, in this contribution we also assess the species composition of the genus Platypterygius, comparing the known anatomy of the type species P. platydactylus with that of the other species previously assigned to the genus. We found that a great morphological affinity in the forelimb of the species P. americanus, P. australis, P. hercynicus, and P. sachicarum (lower Barremian to lower Cenomanian) with that of the type species (Aptian) is unquestionable and differentiate them from all other ophthalmosaurids. Three new diagnostic features (synapomorphies of the genus) support their inclusion in Platypterygius. Consequently, the genus name “Kyhytysuka”, recently proposed to differentiate P. sachicarum from the genus Platypterygius is here rejected. A careful comparison of the cranial characteristics of Platypterygius specimens preserving associated cranial and fin remains demonstrate that Platypterygius is a genus distinguishable from other ophthalmosaurids not only by a typical forelimb but also by a particular combination of skull features. In this context, the skull morphology of “Simbirskiasaurus” concurs with that defining Platypterygius and its minor differences are not sufficient to support its generic distinction. The morphological comparisons and our phylogenetic analysis show the new species P. elsuntuoso more closely related to the Colombian species P. sachicarum than to other species of Platypterygius. Given that the new species came from the lower Barremian and P. sachicarum came from the upper Barremian, the minor differences in dentition found between the two species suggest a speciation by adaptation to new food sources in the Cretaceous epicontinental sea of Colombia.

Characterization of the floral traits, pollen micromorphology and DNA barcoding of the edible flowers from three basil taxa (Lamiaceae)

AbstractThe edible flowers sector is expanding due to the popularity and uses in culinary recipes of different species. In particular, flowers of Ocimum basilicum L. and related taxa are increasingly used for their aromas and nutritional value. However, there is limited information regarding their morphological characteristics and molecular profiles, which are both important to perform a quality control of food, and to avoid contaminations. Hence, our aim was the study of three basil taxa (O. basilicum ‘Cinnamon’, O. basilicum ‘Blue Spice’, and the hybrid O. × africanum Lour.) to obtain data useful for taxa identification and to understand which traits could be linked to their chemodiversity. The plants were grown in a greenhouse starting from seeds. Flowers were collected at anthesis; the morphology of calyxes, corolla and pollen grains was characterized; DNA barcoding analyses were performed. All taxa were identified only as O. basilicum by molecular analyses, but two haplotypes were distinguishable. All taxa were identifiable due to the presence/absence of specific glandular trichomes, and by pollen size and number of colpi. ‘Cinnamon’ and O. × africanum showed more morphological affinities to each other, but histochemical analyses suggested the separation of the three taxa. Pollen grains from ‘Cinnamon’ had the smallest diameter in polar view and were hexacolpate, while ‘Blue Spice’ pollen showed the highest diameter with grains being hexacolpate/octacolpate, similarly to O. × africanum. Our interdisciplinary study provides the first information for authenticating these basil cultivars in packaged products for human consumption.

Unhiding Colombian orchid treasures: A new Lepanthes (Orchidaceae) from the northeastern Andes in Colombia

A new species of Lepanthes from the eastern Colombian Andes from the department of Santander has been discovered. Here it is described, illustrated, and compared with species with morphological affinities, particularly with the sympatric Lepanthes rabei, from which it can be separated by a combination of traits: the larger plant length, reaching up to 8.28 cm in height, the elliptical-lanceolate leaves, concolor, the sepals not denticulate, the lip blades ovate-oblong, obtuse, slightly falcate at the apex, the long cilia at the lip tip and the appendix minute, reflexed downwards, pubescent. Information about its distribution, phenology, and conservation status is also provided.

Impatiens jangjeonense (Balsaminaceae), a new species from South Korea

Impatiens jangjeonense (Balsaminaceae), a new species is reported from the mountainous regions of Gangwon-do province in South Korea. The plant shows morphological affinities with I. hambaeksanensis by means of elliptic or rhomboid-elliptic leaf blade, racemose inflorescence with erect rachises, and white or pinkish-white flower with yellowish and purplish spots, but differs from the latter by bigger flower 1.6–3.9 × 1.1–2 cm vs. 2–2.6 × 1.1–1.6 cm, and spur tip mainly 1- or 2-coiled and deeply biparted vs. mainly non-coiled and slightly biparted. Detailed description of the species along with its colored photographs are provided.

A new species of Tapajosa Melichar from Curaçao, Lesser Antilles (Hemiptera: Cicadellidae: Proconiini)

A new species of the Neotropical genus Tapajosa Melichar 1924 from Curaçao is proposed, described, and illustrated. Tapajosa arawaka sp. nov. differs from other species of the genus mainly in the shape of male genitalia. Its close morphological affinity with Tapajosa spinata Young, 1968 suggests that ancestors of the new species migrated from mainland Venezuela.

A new Anthurium (Araceae) species with silver leaves from Bahia Atlantic Forest, Brazil

Anthurium argyrophyllum, a new species from the Atlantic Forest is described, illustrated and its morphological affinities with other species are discussed. This species is only known from cocoa agroforests in Southern Bahia State, Brazil.

Phylogeny, niche modelling and morphology unveil a new lianescent species of Sigmoidotropis (Leguminosae, Papilionoideae) across rainforest enclaves within Brazilian dry vegetation

AbstractThe small Neotropical papilionoid legume genus Sigmoidotropis (nine species) has been described only relatively recently as the result of the taxonomic rearrangement of the genus Vigna (subtribe Phaseolinae), based on phylogenetic analyses of DNA sequence data. Here, we formally propose a new lianescent species of Sigmoidotropis that occurs disjunctly in enclaves of moist forest of mountainous regions surrounded by dry vegetation at three different localities in the Brazilian states of Bahia and Minas Gerais. Its phylogenetic position and morphological affinities with other genera of Phaseolinae were evaluated by Bayesian inference and maximum likelihood analyses, using sequence data from the nuclear ITS/5.8S and plastid trnK/matK. To better understand this new species’ widely disjunct distribution and a possible connection between its populations in the past, an environmental niche modelling (ENM) analysis was performed. The phylogenetic analyses revealed that Sigmoidotropis appears closest to the genera Ancistrotropis and Delgadoana, together forming the Sigmoid‐keel clade. The new species proposed here is strongly recovered within Sigmoidotropis, being most closely related to S. ekmaniana, S. elegans, and S. megatyla. The ENM indicated changes in the potential distribution of the new species over different climatic periods, with expansion during colder periods and contraction during warmer periods, leading to possible relictual populations in moist forest enclaves. A description of the new species Sigmoidotropis maculata is presented, along with illustrations, a distribution map, taxonomic comments, and conservation status assessment.

Population affinities in pre-colonial West Africa: The case of the burial cave Iroungou (Gabon, 14th-15th century CE).

Our knowledge of the populations of sub-Saharan Africa in the periods before European colonization is limited. Few archeological sites containing human remains have been identified, and written sources for these periods are rare. The discovery in 2018 of the Iroungou sepulchral cave (Gabon), whose use predates the arrival of the Portuguese (14th-15th centuries CE), is an exceptional source of information: at least 28 individuals associated with numerous metal artifacts were found there. The anthropobiological remains were left in situ, but the eight best preserved crania were digitized. This study focuses on the population affinities of these crania, whose morphology was described using 237 landmarks. Geometric morphometric analyses were used to compare the eight Iroungou specimens with 154 individuals representing 12 well-defined African populations. After alignment (Generalized Procrustes Analysis), morphological affinity was assessed using Euclidean and Mahalanobis distances, and posterior probabilities of population membership (discriminant analysis). Results indicate that the eight Iroungou crania have, on average, more affinity with Bayaka Pygmy, followed by Central African Bantu. Nevertheless, individually, the Iroungou specimens show an important morphological variation and the eight crania can be separated into different affinity groups: Bayaka and Central African Bantu, KhoeSan, and East-African Bantu. Finally, one individual presents strong affinity with Somalis. This phenetic mapping of the Iroungou sample raises questions about the profile of the individuals deposited in the cave in a geographical area known for the Loango pre-colonial kingdom, which ruling class seemed to have had privileged relationships with the Pygmy populations.

New quantitative analyses of the Nacholapithecus kerioi proximal ulna confirm morphological affinities with Equatorius and large papionins.

The elbow of Nacholapithecus has been extensively described qualitatively, however its ulnar morphology has never been the focus of an in-depth quantitative analysis before. Hence, our main aim is quantifying the proximal ulnar morphology in Nacholapithecus and exploring whether it is similar to those of Equatorius and Griphopithecus as previously reported. We compared Nacholapithecus proximal ulnar morphology with a sample of extant and extinct anthropoids through principal component analysis and agglomerative hierarchical cluster analysis. Moreover, we calculated the Cophenetic Correlation Coefficient and checked for taxonomical group mean differences through MANOVA and pairwise post-hoc comparisons, as well as the phylogenetic signal in the variables used. The Nacholapithecus ulna displays a moderately long and relatively narrow olecranon, a relatively wide trochlear surface-radial notch width, and a relatively thin sigmoid notch depth. These features resemble those of large papionins and chimpanzees, and some extinct taxa, mainly Equatorius. Results presented here reinforce previous inferences on the functional morphology of the Nacholapithecus elbow, showing adaptations for general quadrupedal behaviors. However, other derived features (e.g., a relatively wide trochlear surface) might be associated with the ape-like traits described for its distal humerus (e.g., wide trochlear groove), thus displaying a combination of primitive and derived features in the proximal ulna. Finally, affinities with large papionins could suggest the presence of some terrestrial habits in Nacholapithecus. However, the lack of evidence in the rest of the skeleton prevents us from suggesting terrestrial affinities in this taxon in a conclusive manner.

Dual-stream multi-dependency graph neural network enables precise cancer survival analysis

Histopathology image-based survival prediction aims to provide a precise assessment of cancer prognosis and can inform personalized treatment decision-making in order to improve patient outcomes. However, existing methods cannot automatically model the complex correlations between numerous morphologically diverse patches in each whole slide image (WSI), thereby preventing them from achieving a more profound understanding and inference of the patient status. To address this, here we propose a novel deep learning framework, termed dual-stream multi-dependency graph neural network (DM-GNN), to enable precise cancer patient survival analysis. Specifically, DM-GNN is structured with the feature updating and global analysis branches to better model each WSI as two graphs based on morphological affinity and global co-activating dependencies. As these two dependencies depict each WSI from distinct but complementary perspectives, the two designed branches of DM-GNN can jointly achieve the multi-view modeling of complex correlations between the patches. Moreover, DM-GNN is also capable of boosting the utilization of dependency information during graph construction by introducing the affinity-guided attention recalibration module as the readout function. This novel module offers increased robustness against feature perturbation, thereby ensuring more reliable and stable predictions. Extensive benchmarking experiments on five TCGA datasets demonstrate that DM-GNN outperforms other state-of-the-art methods and offers interpretable prediction insights based on the morphological depiction of high-attention patches. Overall, DM-GNN represents a powerful and auxiliary tool for personalized cancer prognosis from histopathology images and has great potential to assist clinicians in making personalized treatment decisions and improving patient outcomes.

Phylogenetic position and plastid genome structure of Vietorchis, a mycoheterotrophic genus of Orchidaceae (subtribe Orchidinae) endemic to Vietnam

The orchid genus Vietorchis comprises three species, all discovered in the 21 century. Each of these species is achlorophyllous, mycoheterotrophic and is known to be endemic to Vietnam. The type species of the genus, V. aurea, occurs in a single location in northern Vietnam within a lowland limestone karstic area. Vietorchis furcata and V. proboscidea, in contrast, are confined to mountains of southern Vietnam, far away from any limestone formations. Taxonomic placement of Vietorchis remained uncertain for the reason of inconclusive morphological affinities. At the same time, the genus has never been included into molecular phylogenetic studies. We investigate the phylogenetic relationships of two species of Vietorchis (V. aurea and V. furcata) based on three DNA datasets: (1) a dataset comprising two nuclear regions, (2) a dataset comprising two plastid regions, and (3) a dataset employing data on the entire plastid genomes. Our phylogenetic reconstructions support the placement of Vietorchis into the subtribe Orchidinae (tribe Orchideae, subfamily Orchidoideae). This leads to a conclusion that the previously highlighted similarities in the rhizome morphology between Vietorchis and certain mycoheterotrophic genera of the subfamilies Epidendroideae and Vanilloideae are examples of a convergence. Vietorchis is deeply nested within Orchidinae, and therefore the subtribe Vietorchidinae is to be treated as a synonym of Orchidinae. In the obtained phylogenetic reconstructions, Vietorchis is sister to the photosynthetic genus Sirindhornia. Sirindhornia is restricted to limestone mountains, which allows to speculate that association with limestone karst is plesiomorphic for Vietorchis. Flower morphology is concordant with the molecular data in placing Vietorchis into Orchidinae and strongly supports the assignment of the genus to one of the two major clades within this subtribe. Within this clade, however, Vietorchis shows no close structural similarity with any of its genera; in particular, the proximity between Vietorchis and Sirindhornia has never been proposed. Finally, we assembled the plastid genome of V. furcata, which is 65969 bp long and contains 45 unique genes, being one of the most reduced plastomes in the subfamily Orchidoideae. The plastome of Vietorchis lacks any rearrangements in comparison with the closest studied autotrophic species, and possesses substantially contracted inverted repeats. No signs of positive selection acting on the protein-coding plastid sequences were detected.

<div>A new fossil Malthodes (Coleoptera: Cantharidae) from the Baltic amber Lagerstätte</div>

A new species of soldier beetle (family Cantharidae) from Eocene Baltic amber: Malthodes (s. str.) vallombrosa sp. nov., is described, diagnosed and illustrated. The morphological affinities with the most similar species are discussed. The Eocene Baltic amber was very rich in species of the genus Malthodes. The evolution of these insects probably began after or near the mass extinction that occurred 66 Ma.

The postcranial variability of Quaternary European rhinoceroses: the case study of Stephanorhinus hundsheimensis from the Middle Pleistocene site of Contrada Monticelli (Apulia, southern Italy)

ABSTRACT Five rhinoceros species, mainly distinguishable through cranial, mandibular and dental morphology, were recognized during the Quaternary across Europe. The postcranial morphology of these taxa is quite variable, with strong superposition among species, especially between Stephanorhinus etruscus and Stephanorhinus hundsheimensis. Here, the complete sample including 25 postcranial bones from the early Middle Pleistocene site of Contrada Monticelli (Apulia, southern Italy) is described and compared through morphological and biometric analyses. A few bones, in addition to cranial and mandibular remains from this site, have been previously referred to Stephanorhinus hundsheimensis. We explored the morphological variability of Stephanorhinus hundsheimensis from Contrada Monticelli against the diagnostic characters described in the literature. The size variation of the Contrada Monticelli sample is also investigated, through a comparison with other Quaternary European rhinoceros samples. Our results indicate a wide morphological variability with several distinct characters and a strong morphological affinity between Stephanorhinus etruscus and S. hundsheimensis. Our results suggest that Stephanorhinus hundsheimensis from Contrada Monticelli is a species with a high degree of intraspecific variability, possibly a hybrid population or a population including two morphotypes of the same species.

Neea bradeana (Nyctaginaceae: Pisonieae), a new species with whorled leaf arrangement from the Atlantic Forest of Southeastern Brazil

Neea bradeana is here described for the Atlantic Rainforest of Southeastern Brazil. This new species had been confused for decades with N. verticillata, the type species of the genus which is described for Peru. N. bradeana is characterized by its unbranched shrubs with whorled sessile or short-petiolate leaves and erect-congested inflorescences. This species is here described and illustrated; remarks on taxonomic and morphologic affinities, distribution map, and conservation assessment are also provided.

Using scanning electron microscopy and molecular data to discover a new species from old herbarium collections: The case of Phlomoideshenryi (Lamiaceae, Lamioideae).

Phlomoides is one of the largest genera of Lamiaceae with approximately 150-170 species distributed mainly in Eurasia. In this study, we describe and illustrate a new species, P.henryi, which was previously misidentified as P.bracteosa, from Yunnan Province, southwest China. Molecular phylogenetic analyses revealed that P.henryi is found within a clade in which most species lack basal leaves. In this clade, the new species is morphologically distinct from P.rotata in having an obvious stem and, from the rest, by having transparent to white trichomes inside the upper corolla lip. In addition, micro-features of trichomes on the calyx and leaf epidermis can differentiate the new species from other species grouped in the same clade and a key, based on trichome morphology for these species, is provided. The findings demonstrate that the use of scanning electron microscopy can reveal inconspicuous morphological affinities amongst morphologically similar species and play an important role in the taxonomic study of the genus Phlomoides.

Anaphalis munnarensis (Asteraceae: Asteroideae: Gnaphalieae): a new species from southern Western Ghats, Kerala, India

Anaphalis munnarensis, a new taxon belonging to the family Asteraceae from montane grasslands of Meesapulimala of southern Western Ghats, Kerala, India, is described and illustrated. The new species exhibits some morphological affinities with its allied species Anaphalis meeboldii, but differs in many attributes. A description of the new species along with details on its distribution and ecology, illustration, colour photographs and comparisons with its allied taxa are provided.

Bulbophyllum gopalianum (Orchidaceae), a new species from Mizoram (India), the westernmost region of the Indo‐Myanmar biodiversity hotspot

A new species, Bulbophyllum gopalianum, is described from the montane sub‐tropical forest of Mizoram (India) in the westernmost area of the Indo‐Myanmar biodiversity hotspot. The new species belongs to the section Lemniscata and shows close morphological affinity to B. suavissimum and B. shanicum. A detailed description, diagnosis, phenology, taxonomic notes and conservation assessment are presented. The new species is assessed as critically endangered (CR) based on its very small and restricted population size.

Phylogeny of Prosthechea (Laeliinae, Orchidaceae) based on nrITS and plastid DNA sequences: Reassessing the lumper‐splitter debate and shedding light on the evolution of this Neotropical genus

AbstractAfter a confused taxonomic history, Prosthechea (Laeliinae, Orchidaceae) has been circumscribed in a broad sense to include species previously placed in Encyclia subg. Osmophytum. However, recent alternative classifications resurrecting old names such as Anacheilium and Hormidium, and proposing new genera such as Euchile, Panarica, Pollardia, and Pseudencyclia were presented, splitting Prosthechea into narrower genera. In this study, we provide a molecular phylogeny of Prosthechea to reassess alternative generic classifications and shed light on evolutionary aspects of this highly diverse Neotropical lineage. Eighty species of Prosthechea s.l. and 12 species of related genera were sampled. Bayesian and maximum parsimony analyses of sequences from three plastid regions (rpl32‐trnL, trnD‐trnT, ycf1) and nrITS were included. Possible conflicts between nuclear and plastid data were assessed with partitioned Bremer support. Ancestral state reconstructions of morphological characters relevant to the genus taxonomy are provided. Our results reinforce the monophyly of the lineage corresponding to a broad circumscription of Prosthechea. The small lineages corresponding to Euchile, Panarica, and Hormidium are monophyletic, whereas the larger Anacheilium, Pollardia, and Pseudencyclia are not monophyletic. Splitting Prosthechea into smaller genera is not supported by our phylogeny, and nonmonophyly also hinders the proposal of a comprehensive infrageneric classification. The phylogenetic relationships recovered reflect better the biogeographic patterns than the previously proposed morphological affinities, pointing to the existence of homoplastic floral traits within the genus. Conflicts between nuclear and plastid partitions were detected, suggesting a possible scenario of reticulate evolution.

Sideritis royoi (Lamiaceae): A New Orophilous Species from Northeastern Spain

Sideritis royoi is found in the rocky limestone habitats of the Port Massif (southern Catalonia, Spain). The species was first collected by the local botanist Lluís de Torres in the late part of the 20th century, but the specimens have remained unidentified positively in herbaria for over 40 years. Sideritis royoi likely belongs to section Sideritis subsection Hyssopifoliae and shows some morphological affinities with the relatively widespread South European species S. hyssopifolia L., but it differs from this species because it has subspinescent upper leaves, the main surfaces of its leaves are glabrous or glabrescent, the main abaxial surface of its bracts is without eglandular hairs, and due to the fact that it has shorter inflorescences. Weaker similarities have also been observed with some species belonging to S. subsection Fruticulosae Obón & D.Rivera. In this paper, a description for the new orophilous species is provided, along with a detailed illustration, field photographs, and a comparison with closely related species. We include an assessment of its conservation status and a dichotomous key for the identification of all the species of Sideritis subsection Hyssopifoliae.