Morphofunctional State Of Liver Research Articles

Influence of constant lightning on liver health: an experimental study.

Light pollution has become a serious problem in many urbanized areas of the world. The impact of prolonged exposure to light and consequent disruption of natural circadian rhythms has significant health implications. The current study was undertaken to evaluate the effect of prolonged exposure to light, simulating urban light pollution, on liver health. In order to evaluate the effect of prolonged exposure to light, we examined the morphofunctional state, immunohistochemical and micromorphometric parameters of rat liver in normal conditions and following prolonged lighting exposure. Our results show that nocturnal light disruption triggers a cell death in the liver within 3weeks (necrosis and apoptosis of hepatocytes) and stimulates a change in normal cellular karyometric parameters. At the same time, intracellular regeneration takes place within the organ, which manifests through hepatocyte hypertrophy. Under the influence of constant illumination, the circadian rhythms (CRs) of the size of hepatocytes and their nuclei are restructured, and the rhythm of the nuclear-cytoplasmic ratio is destroyed. The destruction of the CR of expression of p53 and Ki-67 also occurs against the background of the rearrangement of the daily rhythmicity of Per2 and Bmal1. The revealed changes in the morphofunctional state of the liver under the influence of light pollution indicate that a violation of normal illumination regimes is a potent factor leading to significant structural changes in the liver.

Influence of platelet-enriched plasma on the morpho-functional state of liver in rats with induced non-alcoholic steatohepatitis and dyslipidemia

Non-alcoholic steatohepatitis (NASH) is a consequence of the progression of non-alcoholic fatty liver disease (NAFLD), which can lead to cirrhosis or hepatocellular insufficiency. NAFLD is a predictor of cardiovascular diseases (CVD) development. The important factor of the latter development is atherogenic dyslipidemia, and this is a type of 20-80 % NASH patients. The objective: to study the effectiveness of platelet-enriched plasma (PRP) influence on the morpho-functional state of liver in rats with non-alcoholic steatohepatitis. Materials and methods. The study was carried out on 80 adult male Wistar rats. Animals were divided into three groups: I group - animals that received an atherogenic diet for 90 days (n=10); II group - animals with simulated NASH and DL which received a normal diet for 30 days (n=30); III group - animals with simulated NASH and DL and PRP correction (n=30); group of intact animals (n=10). NASH was simulated by introducing an atherogenic diet, which consisted of lard and 50 g / kg of butter for 90 days. PRP was punctured in the tissue of liver, twice at 0.05 ml with 7 days interval. The animals were taken out of the experiment after 90 days of the atherogenic diet and on the 30th day after its cessation or PRP injection. The indicators of body mass, liver mass index (LMI), lipogram, hepatic transaminases were determined, and the further pathological examination of the liver tissue was done. Results and discussion. On the 90th day of the atherogenic diet, the GH level was 3.19 ± 0.56 mmol/l, ALT activity was 118 ± 6.12 U/l, AST 86 ± 4.52 U/l, morphological signs of NASH were detected. On the 30th day, the lipid profile of group II rats did not have statistical differences from group I, in rats after correction of NASH with the use of PRP the level of LLD decreased by 51%, ALT activity - by 54%, AST - by 51% compared to control group (p <0.05), morphologically revealed I - II degree steatosis, focal protein dystrophy. Conclusions. On the 90th day of an atherogenic diet NASH was formed in experimental animals. They had dyslipidemia, which progressed for at least 30 days while maintaining a usual diet. After correction with PRP the level of atherogenic lipoproteins was significantly decreased in rats with NASH and DL; the activity of liver enzymes was lower compared to the group with simulated NASH and DL and the group with progression of NASH and DL for 30 days, morphologically the decrease in liver steatosis and severity of protein dystrophy comparing with a group with simulated NASH and DL and a group with progressive NASH and DL was observed for 30 days.

Disorders of the metabolic status and morphofunctional state of liver and kidneys of chicken

In this study we analyzed biochemical parameters of blood, tibia, histological analysis of liver and kidney tissues of 46‐days old pullets of egg‐laying chickens. The research aim is study of biochemical and morphological changes in replacement pullets to predict risks of growth weak replacement pullets. The research shows that in the serum total proteins were higher then normal range on 28, 8%, albumins on 32,0%. High level of this parameters leads to produce too much uric acid and accumulate it in the body and its higher on 21,3%. Energy change in serum is shown with satisfying range of glucose and reduced rate of total lipids. Sufficient calcium in serum 13,1% with lack of phosphorus. Ca/P ratio exceeds 1.2 times optimal value. Low alkaline phosphatase activity is typical for inadequate bone matrix formation. AST is higher on 25,1% AST and ALT parameters are both high on 25,1% and 15,4% respectively and it means liver function abnormal. There is very low vitamin B2 (42,4% from normal value) wwith sufficiently high carotene supply with vitamins A, E, C in the liver. Liver micronutrients: Zinc – 2,9 times more; Manganese – 3,1 times more with less concentration of Cobalt on 2,6 times and Selenium – 8,3 times. There are calcium 1,6 times less and phosphorus – 1,4 in tibia. Thus in the liver of chickens has focal or diffuse inflammation with blood flow disturbances and of necrosis were recorded. Macroscopic examination found that kidney are oversized, have flabby consistency with light brown color and surface look like almond nut. Microscopic examination found that Bowman's capsule of the cortex kidney is thickened, the space between the capsule and glomeruli is swollen, and the borders of the tubule epithelial cells are poorly distinguishable. The cells are swollen, enlarged and cone‐shaped into the gap make it narrow. The degree of swelling of the epithelial cells, and, consequently, the narrowing of the lumens were uneven. In some tubules, the gaps are still quite distinguishable, in others they look like gaps. There are tubules in which the gaps are almost invisible. Nuclei are not clearly expressed in all cells. The established violations in the interstitial metabolism and structural changes in the parenchymal organs (liver, kidneys) during the restoration of youth is a predisposing load that ensures the body's resistance to adverse environmental influences and loss of productive metabolism during egg production.Micromorphology of the chickens' liver. Impaired blood circulation. Hematoxylin and eosin.X 640Figure 1The architectonics of the glomerular zone of the kidney in chickens 46 days old. Hematoxylin and eosin.X 640Figure 2

Protective effect of adsorbent complex on morphofunctional state of liver during chicken polymycotoxicosis

The presence of mycotoxins in food can lead to pathological effects ranging from acute intoxication to the effects of chronic exposure (such as carcinogenic, mutagenic, teratogenic) in both humans and animals. Mycotoxins are also a major problem in poultry farming and have harmful effects on birds. The experiment was conducted to determine the effect of adsorbents (shungite / zeolite / glucans 0.5% of the diet) added to a birds diet contaminated with mycotoxins on liver enzyme activity, liver weight and histological characteristics, and the antioxidant system. In experiment 60 Cobb 500 broilers of both sexes, divided into three equal groups (20 chicks in each group), live weight of 650-780 g were used. The experimental period was 20 days. The results showed that the addition of a complex of inorganic and organic adsorbents minimized the pathological effect of zearalenone, aflatoxin B1 and T-2 toxin on the liver and the level of enzymes such as alanine transferase (ALT) and aspartate transferase (AST), and also contributed to the reduction of malone dialdehyde levels relative to the toxic control group. Thus, use of a complex of adsorbents offered by us is an effective and ecologically pure strategy of mycotoxin detoxication in protection of the end-user of animal production - the person. How to Cite this Article Pubmed Style Evgenya Yu. Tarasova, Lilia E. Matrosova, Svetlana A. Tanaseva, Nailya N. Mishina, Ramziya M. Potekhina, Olga K. Ermolaeva, Sergey Yu. Smolentsev, Anna M. Tremasova, Ilnur R. Kadikov, Vladislav I. Egorov, Rashid M. Aslanov, Eduard I. Semenov. effect of adsorbent complex on morphofunctional state of liver during chicken SRP. 2020; 11(11): 264-268. doi:10.31838/srp.2020.11.38 Web Style Evgenya Yu. Tarasova, Lilia E. Matrosova, Svetlana A. Tanaseva, Nailya N. Mishina, Ramziya M. Potekhina, Olga K. Ermolaeva, Sergey Yu. Smolentsev, Anna M. Tremasova, Ilnur R. Kadikov, Vladislav I. Egorov, Rashid M. Aslanov, Eduard I. Semenov. effect of adsorbent complex on morphofunctional state of liver during chicken http://www.sysrevpharm.org/?mno=9837 [Access: March 29, 2021]. doi:10.31838/srp.2020.11.38 AMA (American Medical Association) Style Evgenya Yu. Tarasova, Lilia E. Matrosova, Svetlana A. Tanaseva, Nailya N. Mishina, Ramziya M. Potekhina, Olga K. Ermolaeva, Sergey Yu. Smolentsev, Anna M. Tremasova, Ilnur R. Kadikov, Vladislav I. Egorov, Rashid M. Aslanov, Eduard I. Semenov. effect of adsorbent complex on morphofunctional state of liver during chicken SRP. 2020; 11(11): 264-268. doi:10.31838/srp.2020.11.38 Vancouver/ICMJE Style Evgenya Yu. Tarasova, Lilia E. Matrosova, Svetlana A. Tanaseva, Nailya N. Mishina, Ramziya M. Potekhina, Olga K. Ermolaeva, Sergey Yu. Smolentsev, Anna M. Tremasova, Ilnur R. Kadikov, Vladislav I. Egorov, Rashid M. Aslanov, Eduard I. Semenov. effect of adsorbent complex on morphofunctional state of liver during chicken SRP. (2020), [cited March 29, 2021]; 11(11): 264-268. doi:10.31838/srp.2020.11.38 Harvard Style Evgenya Yu. Tarasova, Lilia E. Matrosova, Svetlana A. Tanaseva, Nailya N. Mishina, Ramziya M. Potekhina, Olga K. Ermolaeva, Sergey Yu. Smolentsev, Anna M. Tremasova, Ilnur R. Kadikov, Vladislav I. Egorov, Rashid M. Aslanov, Eduard I. Semenov (2020) effect of adsorbent complex on morphofunctional state of liver during chicken SRP, 11 (11), 264-268. doi:10.31838/srp.2020.11.38 Turabian Style Evgenya Yu. Tarasova, Lilia E. Matrosova, Svetlana A. Tanaseva, Nailya N. Mishina, Ramziya M. Potekhina, Olga K. Ermolaeva, Sergey Yu. Smolentsev, Anna M. Tremasova, Ilnur R. Kadikov, Vladislav I. Egorov, Rashid M. Aslanov, Eduard I. Semenov. 2020. effect of adsorbent complex on morphofunctional state of liver during chicken Systematic Reviews in Pharmacy, 11 (11), 264-268. doi:10.31838/srp.2020.11.38 Chicago Style Evgenya Yu. Tarasova, Lilia E. Matrosova, Svetlana A. Tanaseva, Nailya N. Mishina, Ramziya M. Potekhina, Olga K. Ermolaeva, Sergey Yu. Smolentsev, Anna M. Tremasova, Ilnur R. Kadikov, Vladislav I. Egorov, Rashid M. Aslanov, Eduard I. Semenov. Protective effect of adsorbent complex on morphofunctional state of liver during chicken polymycotoxicosis. Systematic Reviews in Pharmacy 11 (2020), 264-268. doi:10.31838/srp.2020.11.38 MLA (The Modern Language Association) Style Evgenya Yu. Tarasova, Lilia E. Matrosova, Svetlana A. Tanaseva, Nailya N. Mishina, Ramziya M. Potekhina, Olga K. Ermolaeva, Sergey Yu. Smolentsev, Anna M. Tremasova, Ilnur R. Kadikov, Vladislav I. Egorov, Rashid M. Aslanov, Eduard I. Semenov. Protective effect of adsorbent complex on morphofunctional state of liver during chicken polymycotoxicosis. Systematic Reviews in Pharmacy 11.11 (2020), 264-268. Print. doi:10.31838/srp.2020.11.38 APA (American Psychological Association) Style Evgenya Yu. Tarasova, Lilia E. Matrosova, Svetlana A. Tanaseva, Nailya N. Mishina, Ramziya M. Potekhina, Olga K. Ermolaeva, Sergey Yu. Smolentsev, Anna M. Tremasova, Ilnur R. Kadikov, Vladislav I. Egorov, Rashid M. Aslanov, Eduard I. Semenov (2020) effect of adsorbent complex on morphofunctional state of liver during chicken Systematic Reviews in Pharmacy, 11 (11), 264-268. doi:10.31838/srp.2020.11.38

МОРФОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА ПЕЧЕНИ И КИШЕЧНИКА КАРПА ПРИ ИСПОЛЬЗОВАНИИ ОПОКИ В КАЧЕСТВЕ ДОБАВКИ К КОРМУ

The paper investigates the effect of adding to the feed a natural sorbent gaize with concentration of 3% and 6% on fish-biological indicators and morphofunctional state of liver and intestines of scaly cyprin Cyprinus carpio (Linnaeus, 1758). The research was carried out in the innovation center “Bioaquapark - Scientific and Technical Center for Aquaculture” under Astrakhan State Technical University. Feeding of yearlings (0+) of scaly carp was carried out in 3 versions: in one control group and two experimental groups (adding 3% and 6% gaize). It has been stated that adding gaize to combined feed increased the survival rate of individuals from 90 to 100%. The increase in body weight was 53% in the test variant and 11% in the second variant. The morphological picture of carp liver in the control and both experimental groups didn’t differ significantly. There were also no foci of inflammation and pathological changes in the structure of the intestine. The research results prove the possibility of using gaize as an additive in carp diet. The best results were obtained when using 3% mineral feed additive. Inclusion of sorbents in the formulation of feedstuffs increases the resistance of fish to adverse growing conditions, prevention of diseases and producing high-quality and environmentally friendly aquaculture products, as well as significantly reduces the cost of fish products.

Karaciğer sirozu tedavisinde otolog multipotent mezenkimal stromal kök hücresi uygulanması ve sonuçları

Background and Aims:Increase in the number of liver cirrhosis, lack of the sufficient number of donors, high rates of post-operative complications, mortality rates, as well as material and technical shortcomings make search of effective and favorable treatment methods actual. From this point of view, clinical effects of stem cell transplantation captured our attention. The aim of this study is to evaluate the influence of autologous multipotent mesencymal stromal stem cell transplantation on the morphofunctional state of liver during liver cirrhosis. Materials and Methods:For this purpose; experiments were conducted on 49 white male rats (Wistar), 150-180 g weight, minimum of 3 months old. Because they have minimal immunological mismatch in comparison with allogeneic cells, autologous multipotent mesenchymal stromal stem cells were used for the experiments. Liver cirrhosis was modeled in all the experimental animals. To carry out comparative assessment of the effectiveness of the transplantation methods of autologous multipotent mesencymal stromal stem cell, animals were divided into 2 groups. The first group contained 23 rats with autologous multipotent mesencymal stromal stem cell implanted through the portal vein; the second group (24 rats) contained animals with autologous multipotent mesencymal stromal stem cell implanted into the main liver artery. Comparative study of the effectiveness of treatment was carried out based on the morphofunctional state of the liver. Results:The analysis of histomorphologic and biochemical examination results showed higher effectiveness of transarterial transplantation of autologous multipotent mesencymal stromal stem cell into the liver as compared to the intraportal method. It was confirmed by more active regeneration of liver parenchyma, as well as more rapid restoration of functional tests of the liver. Conclusion:Obtained results prove that application of stem cells will be an alternative treatment for organ transplantation in the treatment of liver cirrhosis in the immediate future