Moringa Peregrina Research Articles

Fluctuation of the ecological niche of Moringa peregrina (Forssk.) Fiori with topoclimatic heterogeneity in southern Iran

Heterogeneity can be studied for any dynamic or fixed environmental factors over time. However, determining the extent of heterogeneity occurrence in terms of habitat suitability, variability of dynamic and fixed factors, as well as landform role is an issue that has received less attention. This study aimed to investigate the distribution of Moringa peregrina at two climate change scenarios, to identify the Region of High Heterogenetic (ROHH) of the habitats in those scenarios and to ascertain the heterogeneity of habitat variables of the species in southern Iran. The current and potential distributions of the species in mild and severe climate change scenarios of 2050 and 2070, respectively, were modeled through the Ensemble technique using the climatic and topographic (topoclimatic) variables. The current distribution with four predictions of mild to severe Representative Concentration Pathways (RCP2.5, RCP4.5, RCP6.0 and RCP8.5) were entered into the principal component analysis (PCA) each year to achieve the heterogeneity of distribution. Then, the ROHH was calculated for areas with fluctuations of more than 50%. The topoclimatic variables in the ROHH were compared with the value of each variable in the current distribution in different landforms. The climatic variables of temperature seasonality and mean diurnal range had the greatest impact on M. peregrina distribution. There was more than 90% spatial agreement between the species current and potential distributions under different climate change scenarios (minimum Kappa = 0.9). In climate change scenarios, increase in species distribution is mainly limited by reduced rainfall, high temperature and altitude. The heterogeneity of habitat variables varied greatly in the ROHH and current presence points, indicating the species attempt to occupy new ecological niches. The highest distribution of the species was in the canyons and mountain tops, and the species seeks to occupy these areas in the ROHH. The magnitude of fluctuations of habitat variables at the presence points and the ROHH was different, indicating the species crossing the current niche range to establish in new niche. The mean diurnal range (Bio2) and annual precipitation (Bio12) variables had the lowest heterogeneity in 2050 and 2070 scenarios. This study reports that the fluctuation of habitat variables in areas with high heterogeneity was different from the current distribution range of M. peregrina. No significant fluctuation was found in the distribution range of the species in climate change scenarios.

Antimicrobial Potential of Moringa peregrina Against Some Causative Agents of Urogenital Infections

Abstract
 Aim: To evaluate and compare the antimicrobial effectiveness of aqueous and methanol extracts from Moringa peregrina leaves against standard strains of microorganisms and clinical isolates.
 Study Design: An experimental study which was carried out at the microbiology laboratory, Medicinal and Aromatic Plants Research Institute (MAPRI), Khartoum, Sudan, during the period from September 2012 to March 2013.
 Methodology: Aqueous and methanol extracts of Moringa peregrina at concentration of 100 mg/ml were subjected to antimicrobial screening against six standard strains include Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 53657), Proteus vulgaris (ATCC 6380), Staphylococcus aureus (ATCC 25923), Candida albicans (ATCC 7596) and 133 clinical isolates including bacterial and fungal species. Screening for antimicrobial activity of extracts, standard antibiotics and antifungal drug were detected by the agar well diffusion method. The Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) for the tested extracts were determined by using the broth dilution method. The MDIZs of clinical isolates were analyzed statistically using One-Way analysis of variance.
 Results: Methanol extract of Moringa peregrina leaves revealed prominent activity against standard strains compared to the aqueous extract of the same plant where the diameter of inhibition zone (DIZ) was ranged from 18 mm to 21 mm. The aqueous extract exhibited moderate activity which was ranged from 16 mm to 17 mm. The MIC of leaves methanol extract of Moringa peregrina for standard bacterial strains was ranged from ˂6.25 to 12.50 mg/ml, while the MBC was ranged from 6.25 mg/ml to 50 mg/ml. Standard strain and clinical isolates of Candida albicans was inhibited by 25 mg/ml from methanol extract, while the MFC was 50 mg/ml. The highest MDIZs obtained by the leaves methanol extract of Moringa peregrina on the clinical isolates was 18.93±3.07 mm for Escherichia coli, 18.50±2.07 mm for Pseudomonas aeruginosa, 18.62±2.72 mm for Proteus mirabilis and 18.11±2.47 mm for Staphylococcus saprophyticus. The antifungal activity of leaves methanol extract of Moringa peregrina on clinical isolates of Candida albicans was 16.56±1.62 mm which was lower than activity of Candizole 5 mg/ml. Moringa peregrina leaves extract showed varied MICs values towards clinical isolates which was ranged from 6.25 mg/ml to 25 mg/ml, while the MBC of the same isolates was from 25 mg/ml to 50 mg/ml.
 Finally, Methanol extract of Moringa peregrina was found to be effective against both the standard strains and clinical isolates compared to the aqueous extract.

Nutritional and therapeutic effect of Moringa peregrina on lead and cadmium – induced toxicity in rats compared with EDTA

The study was investigated to determine the Therapeutic effect of Moring peregrina (Forssk) seed powder at dose of 75 /mg/kg Bw./day comparis to calcium Disodium Ethylene – Diamine Tetra-Actic Acid CaNa2 EDTA at adose of 75/mg/Kg Bw oral post exposed to lead acetate or cadmium ehloride at adose 50/mg/K Bw orally for 6 weeks. 40 male rats divided into 9 group: G1= no treatment , G2 and G3= treated with lead or cadmium , G4= exposed to lead acetate for 6ws then M. peregrina for 2ws , G5= exposed to cadmium for 6ws then M. Peregrina for 2ws , G6 and G7= exposed to lead or cadmium for 6ws then CaNa2 EDTA for 2ws , G8 and G9 = exposed to lead or cadmium for 6ws then M. peregrina + CaNa2 EDTA . Results showed significant decrease (P≤ 0.05) in weight gain of rat in (G2 , G3 , G6 , G7 , G8 , G9).a Significant decrease (G4 and G5) relative to no treated animals. The hematological parameters showed a significant decrease in hemoglobin Hb Red Blood Cells (RBC) , Packed Cell Volume (PCV), while significant increase in white Blood Cells (WBC) , mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH) and lymphocytes.
 In (G2, G3, G6, G7, G8, G9) on the otherhand administration of seed M. Peregrina showed levels . also the results showed statistically significant increase in Blood glucose, Urea, Creatinine, Total protein , Albumin ,and activity of Aspartate Amino Tranfears (AST) ,and Alanine Amino transFears (AST). In (G2, G6, G7, G8, G9) while in (G3) showed the same result except Blood protein, significant in creased lead and cadmium toxicity increased Alkaline phosphatase (ALP) but CaNa2 EDTA decreased ALP. On the other hand we notesd enhacement result in (G4 and G5). Theirs a significant decrease in reduced glutathione (GSH) level in (G2 , G3 , G6 , G7 , G8 , G9) but no significant decrease happend in (G4 and G5). Further studies thus can be recommended for protective role of M. peregrina seed powder against toxic effect of lead , cadmium compare to side effect of CaVa2 EDTA during chelation therapy.

Reduction of reproductive toxicity in murine sperm model using Moringa peregrina leaves extracts

Reduction of reproductive toxicity in murine sperm model using Moringa peregrina leaves extracts

Investigating the Expression of Genes Involved in Apoptosis and Mutation in Ovarian Cancer Cell Line and Possible Hepatotoxicity in Rats Induced by New Green Synthesized Ag-NPs

Silver nanoparticles (Ag-NPs) can enter tumor cells through endocytosis and transfer to mitochondria, then disrupt mitochondrial function, damage deoxyribonucleic acid (DNA), and finally lead to cell death and apoptosis. As the Ag-NPs synthesized by Moringa peregrina leaf extract contain biomolecules such as flavonoids and terpenoids, they may suppress cancer by disrupting the flow of autophagy and inhibiting the differentiation of cancer cells, especially in apoptosisresistant cancer cells. Accordingly, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromid (MTT) assay proved that Ag-NPs could inhibit the OVCAR-3 cell line in a time- and concentrationdependent manner. Real-time polymerase chain reaction (PCR) results showed that the ratio of the pro-apoptotic Bak1 gene to the anti-apoptotic Bclx gene increased about 10 times compared to the control. Also, the expression of the caspase-3 gene has increased about 18 times compared to the control. In addition, it was found that Ag-NPs can inhibit the progression of ovarian cancer cell lines and decrease the expression of the AKT1 gene by 0.08. And the more important point was the safety of Ag-NPs, which was checked by the lipid peroxidation method and it was confirmed that the treated hepatocytes did not have any significant difference in the production of malondialdehyde with the control and probably do not cause hepatotoxicity.

Effect of irrigation frequency and selenium fertilization on the vegetative growth and biomass yield of Moringa oleifera and Moringa peregrina

To maximize the production value of Moringa species, there is a need to understand the morphological effect of irrigation frequency and Se fertilizer levels on Moringa species' growth and development. Hence, the aim of this trial was to determine the combined effect of irrigation frequency and Selenium (Se) fertilizer levels on the growth of Moringa oleifera (M. oleifera) and Moringa peregrina (M. peregrina) in the central region of the Kingdom of Saudi Arabia. A split-plot arrangement was used, where the treatments were arranged in a Completely Randomized Block Design (CRBD) with three replicates, as the study included two plant species (M. oleifera and M. peregrina), four irrigation frequencies (7, 10, 15, and 20 days), and three Se levels (0.0, 12.5 and 25 mg/L foliar spray). M. peregrina showed slow emergence and plant establishment as compared to M. oleifera. The results indicated that plant height, leaves and stems fresh weight increased with the increase in Se level, and were highest when the two plant species were irrigated every 10 days which surpassed all other irrigation frequencies. The findings of this research study indicated that the two Moringa species, particularly M. oleifera could successfully be grown using drip irrigation at a 10-days irrigation frequency.

Investigation of the nephroprotective activity of Moringa peregrina leaves aqueous extract in mice

Traditional remedies for Moringa peregrina leaves have a variety of uses with confirmed biological and therapeutic effects, as per published reports. The current study aims to evaluate the ability of the leaves aqueous extract to protect from nephrotoxicity in gentamicin-treated mice. Phytochemical analysis for the aqueous extract was performed using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay for antioxidants, Folin-Ciocalteu, AlCl3 and HPLC- MS/MS analysis, focusing on phenol and flavonoid content. The nephroprotective activity of the prepared extract was evaluated by means of variable biochemical parameters including Creatinine (Cr), Uric Acid (UA), and Urea (Ur). In addition, histological examination of renal tissues was performed in all mice groups (control, gentamicin-induced (150 mg/Kg i.p) and aqueous extract-orally treated groups (500 and 1000 mg/Kg)). Findings reveal that the prepared extract has total phenols (555.57±0.92mg/g, equivalent to gallic acid), flavonoids (40.08±1.56 mg/g, equivalent to quercetin), and DPPH IC50 (3.10 µg/ml). HPLC-MS/MS analysis revealed the presence of 10 phenols and flavonoids compounds. In vivo studies showed a significant (P < 0.05) reducing effect for the high-dose treatment, on serum and urine concentrations for UA, Cr, and U, among the nephrotoxicity induced mice. Low-dose treated group showed significant reduction on serum concentration of UA, Cr and U, but only for Cr concentration in urine. The histological examination showed an improvement in the image of the renal tissue among the induced-nephrotoxicity mice, which was treated with high-dose extract. In conclusion, leaves aqueous extract of M. peregrina have shown potential protective effect to counteract some of the gentamicin consequences on kidney functions.

Oil Extracted Moringa peregrina Seed Cake as a Feed Ingredient in Poultry: A Chemical Composition and Nutritional Value Study.

The chemical composition, antioxidant activity, tannic acid content, mineral, fatty acid, and amino acid profiles of oil-extracted Moringa peregrina seed meal (OEMPSM) were determined. Apparent (AME) and true (AMEn) metabolizable energy and apparent (AAAU) and true (TAAU) amino acid utilization were evaluated using a precision feeding trial. The protein (CP) quality was evaluated by a total efficiency analysis method. The antioxidant activity, gauged by 2,2-diphenyl-1-picrylhydrazyl (DPPH), was 237, 353, and 15.2 mg/mL for the water and ethanol extracts, and ascorbic acid, respectively. Tannic acids were 131.4 mg/100 g dry weight. The OEMPSM had 27.2% CP and 22.4, 15.1, and 15.8 MJ/kg of gross energy, AME and AMEn, respectively. The neutral detergent fiber, acid detergent fiber, and hemicellulose were 40.2, 29.7, and 10.5% DM, respectively. The 15.41% of total fatty acids were saturated and 84.57% unsaturated. The AAAU and TAAU of OEMPSM were 30.92% and 61.06%, respectively. From findings, OEMPSM comprises a valuable level of bioactive substances, amino acids, fatty acids, minerals, and energy; it can provide up to 1.12% of the requirements of total amino acids of chickens (1-21 days); however, the quality of its protein was found to be 44.6% less than that of protein of soybean meal.

Evaluating the effect of Moringa peregrina (Forssk.) Fiori seeds on some biochemical and oxidative stress markers of obese experimental rats.

Evaluating the effect of Moringa peregrina (Forssk.) Fiori seeds on some biochemical and oxidative stress markers of obese experimental rats.

Characterization of Phytochemical and Nutrient Compounds from the Leaves and Seeds of Moringa oleifera and Moringa peregrina

Moringa oleifera and M. peregrina are robust and fast-growing trees. These trees are considered some of the most highly valued trees worldwide because of their properties and uses. This study aimed to investigate and determine the content of phytochemical and nutrient compounds in the fresh leaves and the mature seeds of both M. oleifera and M. peregrina. The experimental data analysis showed that all four extracts were rich in proximate minerals, fatty acids (FA), and un-saponified and polyphenolic components. The total percentages of saturated and unsaturated fatty acids compounds obtained from the leaves and seeds of M. oleifera and M. peregrina were (45.02 and 54.93%), (10.80 and 89.19%), (37.13 and 62.8%), and (11.95 and 88.03%), respectively. The major polyphenols compositions were identified as gallic acid, chlorogenic acid, methyl gallate, and coffeic acid. The outcomes of the present study indicate that the leaves and seeds of Egyptian Moringa (M. oleifera and M. peregrina) contain various phytochemical and nutrient compounds, which can provide several health advantages and play an important role in the metabolism of the human body, especially in diseases such as atherosclerosis, heart disease, obesity, and high cholesterol and triglycerides.

Comparison of Antioxidant and Alpha-Glucosidase Inhibitory Properties of Moringa peregrina and Ferulago carduchorum Leaf Extracts and Microencapsulation of Superior Plant

Today, medicinal plants have a crucial role in treating diseases such as diabetes and cancer. These plants do not impose any side effects owing to their bioactive compounds in comparison with chemical drugs. Several studies have demonstrated antioxidant capacity and α-glucosidase inhibitory effects of bioactive compounds from medicinal plants. According to previous studies, Moringa peregrina (MP) and Ferulago carduchorum (FC) are two promising plants in terms of antioxidant capacity and α-glucosidase inhibitory effects. This research followed a three-stage study. In the first stage, the antioxidant and α-glucosidase inhibitory properties of MP and FC ethanolic extracts, native to Iran, were compared using spectrophotometric methods. The results showed that the ABTS•+ radical scavenging and α-glucosidase enzyme inhibitory activities of both plants were dependent on extract concentration. MP exhibited lower IC50 values in both tests, 1.01 and 4.96 mg·mL−1, respectively. Accordingly, the extract of MP was selected for further experiments. In the second stage, total phenolic content (TPC) and GC-MS analysis were conducted on MP extract to investigate the reason behind its antioxidant capacity and α-glucosidase inhibitory properties. Results of assessing total phenolic content (TPC) using the Folin-Ciocalteu method revealed a strong positive correlation between TPC with antioxidant activity (r = 0.94, p < 0.05 ). GC-MS was used to identify phytoconstituents of the extract, leading to the determination of 35 components whose major one was vitamin E (10.2%). To ensure its suitability for food fortification, in the third stage, encapsulation of the MP extract was followed. Microencapsulation was performed using three polymer coatings, and the effects of carriers were investigated on moisture content, solubility, bulk density, microencapsulation yield, particle size, antioxidant activity, and TPC. According to the experiments, antioxidant activity and TPC were retained well in all carriers. Moreover, SEM, DSC, and FTIR analyses confirmed that the extract was well-coated and no surface fractures were observed. The results indicated that MP can be a promising plant for food fortification as a natural antioxidant and antidiabetic source.

Inhibition of crystallization of calcium oxalate monohydrate using leaves from different species of Moringa – Experimental and theoretical studies

The effect of Moringa leaves on the inhibition of crystallization of calcium oxalate, a key component of kidney stone, has been assessed by experimental and theoretical methods. Specifically, methanol-water extracts of Moringa oleifera and Moringa peregrina leaves have been considered as inhibitors for the crystallization of calcium oxalate monohydrate. Studies based on turbidity measurements, microscopy of crystals, as well as calcium content in mixtures with and without the leaf extracts have revealed greater inhibitory potential in the case of M. oleifera as compared to M. peregrina. Modelling of the interactions between a representative fragment of calcium oxalate monohydrate with selected phytochemicals present in both leaves enables us to propose a possible mechanism of inhibition.

Development of a Citric-Acid-Modified Cellulose Adsorbent Derived from Moringa peregrina Leaf for Adsorptive Removal of Citalopram HBr in Aqueous Solutions.

A citric-acid-modified Moringa peregrina leaf substrate was prepared and studied as an effective adsorbent for the adsorptive removal of citalopram HBr (CTM). FTIR spectra were utilized to characterize the prepared solid. The effects of experimental variables on the percentage removal of citalopram HBr were investigated using response surface methodology. The optimum conditions selected for removal of CTM were 7 and 4 min, 0.17 g per 50 mL and 35 mg·L−1 for pH, contact time, adsorbent dose and initial concentration of CTM, respectively. Under the optimized experimental conditions, 82.59% CTM (35 mg·L−1) was removed. The Langmuir isotherm, Freundlich isotherm, pseudo second-order kinetic model and diffusion-chemisorption model explained the adsorption data successfully. The maximum adsorption capacity at 298 K was 8.58 mg·g−1. A thermodynamic study illustrated that CTM adsorption was spontaneous and endothermic in nature.

Antimicrobial activity of moringa peregrina seed oil: chemical composition and effect of extraction procedure

Background and objective: The importance of new sources of oils has recently emerged, one such source being Moringa. There is an urgent need to choose the most appropriate extraction method regarding its impact on the properties and stability of the extracted oil.The objective of this research work is to study the impact of two extraction methods on the chemical and thermal properties as well as the antimicrobial activity of Moringa peregrina seed oil.Methods: Moringa peregrina seeds were collected from their original growing locations in Sudan. Cold pressing (CP) and maceration inn-hexane (MH) was used to extract the oil, and their yields were found to be 9.12% and 21.87%, respectively. Chemical properties of the oil were studied and in each case triplicate analyses were completed. The mean and the standard deviation were then determined (mean±SD).Results: Besides the peroxide value, the chemical properties of the oil extracted by the two methods remained relatively unchanged. Additionally, the results revealed a higher activity of the cold pressed oil against all tested organisms (Bacillus subtilis, Staphylococcus aureus Escherichia coli, Pseudomonas aeruginosa, Candida albicans) compared to the one obtained by maceration method. Furthermore, the thermogravimetric analysis and derivative thermogravimetric analysis (TGA and DTGA) demonstrated significant variations in the onset of degradation between the oil samples extracted by the two methods: 200◦C (cold pressing) against 274◦C (maceration). The maximum mass loss for the oil extracted by the two methods does not differ significantly, and the final degradation temperature was similar. Conclusion: The differential scanning calorimeter (DSC) thermograms for the oil extracted by the two methods showed somewhat similar characteristics regarding the heating curves, while the cooling curves differed considerably. Keywords: Antimicrobial activity; Cold press; Maceration; Moringa peregrina; Thermal properties.

Evaluating anti-obesity potential, active components, and antioxidant mechanisms of Moringa peregrina seeds extract on high-fat diet-induced obesity.

There are no medical drugs that provide an acceptable weight loss with minimal adverse effects. This study evaluated the Moringa peregrina (MP) seed extract's anti-obesity effect. Twenty-four (6/each group) male Sprague Dawley rats were divided into group Ι (control), group ΙΙ (high-fat diet [HFD]), group ΙΙΙ (HFD+ MP [250 mg/kg b.wt]), and group ΙV (HFD+ MP [500 mg/kg b.wt]). MP administration significantly ameliorated body weight gains and HFD induced elevation in cholesterol, triglycerides, LDL, and reduced HDL. Moreover, MP seed oil showed high free radical-scavenging activity, delayed β-carotene bleaching and inhibited lipoprotein and pancreatic lipase enzymes. High-performance liquid chromatography (HPLC) revealed three major active components: crypto-chlorogenic acid, isoquercetin, and astragalin. Both quantitative Real-time PCR (RT-PCR) and western blotting revealed that MP seeds oil significantly decreased the expression of lipogenesis-associated genes such as peroxisome proliferator-activated receptors gamma (PPARγ) and fatty acid synthase (FAS) and significantly elevated the expression of lipolysis-associated genes (acetyl-CoA carboxylase1, ACCl). The oil also enhanced phosphorylation of AMP-activated protein kinase alpha (AMPK-α) and suppressed CCAAT/enhancer-binding protein β (C/EBPβ). In conclusion, administration of M. peregrina seeds oil has anti-obesity potential in HFD-induced obesity in rats. PRACTICAL APPLICATIONS: M. peregrina seeds oil had a potential anti-obesity activity that may be attributed to different mechanisms. These included decreasing body weight, and body mass index and improving lipid levels by decreasing total cholesterol, triglycerides and LDL-C, and increasing HDL-C. Also, M. peregrina seeds oil regulated adipogenesis-associated genes, such as downregulating the expression of (PPARγ, C/EBPα, and FAS) and improving and upregulating the expression and phosphorylation of AMPKα and ACCl. Despite that M. peregrina extract has reported clear anti-obesity potential through animal and laboratory studies, the available evidence-based on human clinical trials are very limited. Therefore, further studies are needed that could focus on clinical trials investigating anti-obesity potential different mechanisms of M. peregrina extract in humans.

Potential benefits of Moringa peregrina defatted seed: Effect of processing on nutritional and anti-nutritional properties, antioxidant capacity, in vitro digestibility of protein and starch, and inhibition of α-glucosidase and α-amylase enzymes

This study aimed to eliminate the bitter taste of Moringa peregrina press cake (MPC) as a byproduct of oil extraction (by employing safe and conventional methods) and evaluating its potential for formulating value-added food products. The characteristics (nutritional and anti-nutritional properties, monosaccharide composition, in vitro starch and protein digestibility, antioxidant capacity, and in vitro α-glucosidase, and α-amylase inhibitory activity) of raw, debittered (soaked in distilled water and boiled), and roasted (after debitterization) MPC flours were determined. Debitterization significantly increased total protein, fiber, arabinose, xylose, antioxidant activity, in vitro protein digestibility, and α-amylase inhibitory activity, whereas it decreased total starch, resistant starch, starch digestibility, ash, glucose, phytic acid, tannin, and oxalate contents. Fiber content, protein digestibility, α-amylase inhibitory activity, and antioxidant activity were further increased as the result of roasting. MPC and its products could inhibit α-amylase activity, with the highest inhibition belonging to roasted debittered samples. The current study is the first to report on the comprehensive nutritional and bio- and physicochemical aspects of Moringa peregrina press cake and the effect of treatments on improving its sensorial, nutritional, and health-promoting properties. Therefore, these results indicate the potential of treated MPC as a novel natural functional ingredient for various food formulations.

Phyto-Decoration of Selenium Nanoparticles Using Moringa peregrina (Forssk.) Fiori Aqueous Extract: Chemical Characterization and Bioactivity Evaluation

Selenium is an important trace element as a part of the seleno-proteins, which regulate the oxidative status of the body and is required for the normal physiological reaction. Biogenic synthesis of selenium nanoparticles (SeNPs) using plant extracts is recommended over microorganisms or chemical reactions. Herein, SeNPs were synthesized using Moringa peregrina leaves extract (MPM-SeNPs). The purified nanoparticles were chemically characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), and energy-dispersive X-ray (EDX) analyses. The biological activity of MPM-SeNPs was evaluated by testing their antibacterial and antioxidant activities. Their anti-proliferative effect against breast cancer and normal fibroblast cell lines was also investigated. The highest absorption of biogenic MPM-SeNPs is 279 nm, and FT-IR analysis indicated peaks shifting and bending according to phenols, polysaccharides, and protein moieties. The Se-element made up 73.2 percent of the nanoparticles sample; they are agglomerated spheres with smooth surfaces and negative electric charges on their surfaces, with a diameter range of 80-150 nm. MPM-SeNPs revealed antibacterial activity against Gram-positive bacteria with MIC values of 20-50 µg/mL and radical scavenging potential at IC50 23.6±0.2 µg/mL and 25.6±1.7 µg/mL in DPPH and ABTS assays, respectively. Moreover, the nanoparticles exhibited cytotoxic activity against breast cancer cell lines with an IC50 value of 129.4±4.4 µg/mL. MPM-SeNPs might have a potential role in the green synthesis of chemotherapeutic drugs and could be used as a natural agent in the food and pharmaceutical industries.

Removing Acidic Yellow Dye from Wastewater Using Moringa Peregrina

Removing Acidic Yellow Dye from Wastewater Using Moringa Peregrina

Ben Tree (Moringa peregrina) Oil and Its Uses in the Land of Israel

Ben Tree (Moringa peregrina) Oil and Its Uses in the Land of Israel

Anti-proliferative, Anti-angiogenic and Anti-inflammatory Effects of Moringa peregrina Leaf Extracts on Testosterone- Induced Benign Prostatic Hyperplasia in Rats.

Aim:To investigate the potential anti-inflammatory and biochemical effects of Moringa peregrina leaf extracts on testosterone-induced benign prostatic hyperplasia (BPH) in rats. Methods:Six groups of rats (each group included 5 rats) were included in this study. The groups included: 1) the control group, 2) the testosterone-induced BPH group, 3) with 50 mg/kg bwt (bodyweight) oil-treated BPH, 4) with 100 mg/kg bwt. oil-treated BPH, 5) with 500mg/kg bwt. ethanol treated BPH and 6) with 1,000 mg/kg bwt. aqueous treated BPH group. Biochemical markers were measured to evaluate the effect of M. peregrina leaf extracts.Results:Our results showed a significant improvement in the thickness of epithelial cells of the BPH glandular tissues when treated with different M. peregrina extracts (p < 0.05). In addition, M. peregrina extracts showed anti-inflammatory, anti-proliferative and anti-angiogenesis effects on the BPH tissues by reduction of IL-6, PCNA and VEGF-A, respectively. Conclusion:Our preclinical study concluded that M. peregrina leaf extracts showed a significant effect on BPH by reducing inflammation, proliferation, and angiogenic processes with no signs of toxicity.