Moringa Oleifera Seed Oil Research Articles

Microwave-Assisted vs. Conventional Extraction of Moringa oleifera Seed Oil: Process Optimization and Efficiency Comparison.

This study aims to evaluate the effectiveness of microwave-assisted and conventional extraction using ethanol, hexane, and petroleum ether as solvents, and to optimize the process for extracting oil from Moringa oleifera Lam. seeds, with a focus on improving food-grade oil production. Response surface methodology (RSM) was applied to enhance the extraction process of the oil. Central composite rotational design (CCRD) was used to analyze the impact of solid-liquid ratio (x1), power (x2), and temperature (x3) on oil yield. The optimization identified the optimal conditions as a solid/liquid ratio of 1:38, power of 175 W, and temperature of 50 °C, achieving a 42% oil yield. Notably, the microwave-assisted extraction reduced the processing time from 8 h (using conventional Soxhlet extraction) to just 1 h. Conventional extraction with hexane and petroleum ether was also performed for comparison, resulting in similar oil content and fatty acid profiles, predominantly, oleic acid. FTIR analysis confirmed that the microwave-extracted oil contained fatty acids and had similar characteristics to the conventionally extracted oil. Thus, the use of ethanol as a green solvent in the microwave has shown significant improvement in terms of time and energy savings compared to the Soxhlet method with toxic solvents. This study concludes that microwave-assisted extraction with ethanol provides a more energy efficient, environmentally friendly, and time-saving alternative for food-grade oil production, aligning with advancements in food engineering and production.

Extraction, Characterization and Evaluation of Health Applications of Moringa oleifera Seed Oil

In this study, the seeds of Moringa oleifera (MO) plant grown in the Adamawa Region of Cameroon were valorized by extracting their oil using mechanical expression. The oil was allowed to clarify through by gravity and an oil yield of 26.07% was obtained. The extracted MO oil was subjected to physicochemical characterization giving the following results; the density of the oil was 0.896 g/mL, a refractive index of 1.468, a viscosity of 49.80± 0.30 mPa.s, an acid value of 2.69%, a peroxide value of 3.7 meq of O2/kg of oil, a saponification value of 189.34 mg of KOH/g of oil and an iodine index of 67.79g/100g of oil. The determination of relevant secondary metabolites by titration revealed a flavonoid content of 0.66 mg GAE/g, total phenol content of 9.7 mg QE/g, carotenoid content of 0.25 mg beta-caro/g and antioxidant activity gave an IC50 concentration of 17.5 mg/mL. Determination of the fatty acid profile revealed that oleic acid (78.45%) makes up the predominant component in the MO seed oil. The chemical composition of the MO seed oil was further verified by IR spectroscopy whose spectral interpretation revealed a predominance of fatty acid moieties in the oil. From these results, it appears that Moringa oleifera seed oil has moisturizing, antioxidant, anti-inflammatory and nourishing properties and therefore the oil is a potent raw material or bioactive substance in the formulation of cosmetic products like creams for body lotion to combat skin infections and the MO seed can find pharmaceutical applications amongst others.

Fatty Acid Profile and Physicochemical Properties of Moringa oleifera Seed Oil Extracted at Different Temperatures.

Moringa oleifera Lam. (Moringaceae) is a tropical plant native to India. It is widespread throughout the southern hemisphere, with great adaptability to high temperatures and water scarcity. Its seeds have a great amount of oil with a high content of oleic acid, quite similar to olive oil. Therefore, this study is focused on the extraction of oil from moringa seeds via an automatic screw press extractor at different temperatures (70, 100, 130, 160, 190, and 220 °C) and on the analysis of its acidity, acid value, peroxide value (PV), saponification value (SV), iodine value (IV), optical properties, and fatty acids profile. The results showed that the oil yield was 19 ± 3% regardless of the temperature applied. The oil was stable from the oxidative point of view, with a high acidity. Temperature extraction did not significantly affect the SV and the IV. However, the extraction temperature should be below 190 °C to obtain a translucent and luminous oil with light yellow tones. The oil contains high levels of unsaturated fatty acids, especially oleic acid (ω9) (up to 77.8%) and linolenic acid (ω3) (3.4%). On the other hand, behenic (7%), palmitic (6%), stearic (5%), and arachidic (0.2%) were the dominant saturated acids. The good properties of moringa oil make it a good, sustainable alternative to vegetable oils.

The Effect of Adding Different Proportions of Local Moringa oleifera Seed Oil on the Shelf Life Extension of Flaxseed Oil

The Effect of Adding Different Proportions of Local Moringa oleifera Seed Oil on the Shelf Life Extension of Flaxseed Oil

Effect of Moringa oleifera Leaf Powder and Seed Oil on Insect Pests of Stored Maize and Cowpea

The leaf powder of Moringa oleifera was applied as direct admixture at 5g, 10g and 15g while the seed oil was applied at 5ml and 10ml to 100g each of Maize and Cowpea grains respectively, while the control was without insecticide application to test for insect mortality., progeny development and damage caused on stored maize and cowpea (Sitophilus zeamais and Callosobruchus maculatus). The experiment was laid out in a Completely Randomized Design (CRD) and results obtained were subjected to analysis of variance (ANOVA) while means were separated using the Least Significant Difference (LSD). The result showed significantly (P<0.05) progeny reduction of adult Sitophilus zeamais and Callosobruchus maculatus using Moringa oleifera leaf powder at different concentration 0, 5g (23.90, 28.62), 10g and 15g respectively. Control (0) recorded the highest number 68.51 in Callosobruchus maculatus, and 56.85 in Sitophilus zeamais. Mortality test result showed that moringa leaf powder significantly(P<0.05) increased the mortality of adult S. zeamais and C. maculatus with increase in concentration after 96 hours of exposure while moringa seed oil offered 100% mortality after 24 hours of exposure. Germination of maize and cowpea was not affected after treatment with moringa leaf powder and seed oil. Contact toxicity result showed significant adult mortality of S. zeamais and C. maculatus on filter paper with 15% mortality in C. maculatus and 10% in S. zeamais. Damage was significantly reduced in treated grains compared with the untreated control. Moringa seed oil was more toxic to S. zeamais and C. maculatus compared to moringa leaf powder and the untreated control. The protective effect of moringa seed oil against S. zeamais and C. maculatus comes principally through oviposition suppression and reduced or complete inhibition of adult emergence, consequently reducing or eradicating infestation risk, damage and loss of maize and cowpea grains in storage. While the use of plant essential oils against storage insect pest is being advocated, the availability, affordability and the tendency of tainting protected grains should be considered.

Extraction and Comparative Evaluation on the Physicochemical Characteristics of Yemeni Moringa oleifera Seeds Oil

Moringa oleifera is a versatile tree with nutritional, medicinal and environmental benefits making it a valuable resource for communities in many regions of the world. This study aimed to extracted oil from Yemeni Moringa oleifera seeds oil (YMOSO) using two methods (cold pressing and seoxhlet n-hexane). The yields of the oils were 16.12% and 28.34, % respectively. The yields of the oils were 16.12% % and 28.34% respectively. Where it was investigate the chemical and physical characteristics by two method cold press and soxhlet n-hexane showed of Yemeni Moringa oleifera seeds oil (YMOSO), including a free fatty acid (FFA) content of 1.12 % and 2.98 %, an acid value of 5.99±0.5 mg/g and 6.52±0.5 mg/g, an iodine value of 67.68±0.1 mg/g and 67.43±0.1 mg/g, a saponification value of 189.51±0.1 mg/g and 188.49±0.1 mg/g, a refractive index of 1.46±0.01 and 1.47±0.01 at 25 °,C a moisture content of 0.24±0.03% and 0.36±0.03%, a density of 0.90±0.05 g/ml and 0.89±0.05 g/ml at 25 °C, a specific gravity of 0.968±0.03 g/ml and 0.956±0.03 g/ml at 25 °C, and a viscosity of 48 and 47 cP at 25 °C respectively. the chemical components of the oil were determined using Fourier transform infrared (FTIR) spectroscopy and Gas Chromatography-Mass Spectroscopy (GS-MS).

Characterization of Moringa oleifera Seed Oil for the Development of a Biopackage Applied to Maintain the Quality of Turkey Ham.

Moringa oleifera has a high level of active chemicals that are useful in the food industry, and they have antibacterial and food preservation properties. The characterization of M. oleifera seed oil (MOS) may vary due to agronomic and environmental factors. Therefore, it was necessary to know the composition of lipids present in our oil extracted under pressing at 180 °C and thus determine if it is suitable to produce a biopackaging. Within the characterization of the oil, it was obtained that MOS presented high-quality fatty acids (71% oleic acid) with low values of acidity (0.71 mg KOH/g) and peroxide (1.74 meq O2/kg). Furthermore, MOS was not very sensitive to lipoperoxidation by tert-butyl hydroperoxide (tBuOOH) and its phenolic components, oleic acid and tocopherols, allowed MOS to present a recovery of 70% after 30 min of treatment. Subsequently, a biopackaging was developed using a multiple emulsion containing corn starch/carboxymethylcellulose/glycerol/MOS, which presented good mechanical properties (strength and flexibility), transparency, and a barrier that prevents the transfer of UV light by 30% and UV-C by 98%, as well as a flux with the atmosphere of 5.12 × 10-8 g/ m.s. Pa that prevents moisture loss and protects the turkey ham from O2. Hence, the turkey ham suffered less weight loss and less hardness due to its preservation in the biopackaging.

Phytochemical Profiling and Pharmaceutical Properties of Moringa oleifera Leaves Powder and Seed Oil Against Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) is one of the deadliest types of cancer with a mortality rate of 8.9% of the total cancer deaths in Indonesia. This cancer can be caused by exposure to hepatitis B and C viruses, NAFLD, autoimmune, diabetes to sporadic genetic diseases. The development of chronic HCC is generally preceded by the occurrence of severe liver fibrosis and cirrhosis. One of the genes that play a role in fibrosis in the incidence of HCC is TGF-β1. As a pro-fibrotic cytokine, the presence of high levels of TGF-β1 may be due to oxidative stress activity early in cancer development. One of the natural ingredients with lots of phytochemical content in the form of antioxidants that can reduce this activity is Moringa plant (Moringa oleifera). In this study we used a computational approach using molecular docking on the results of the GC-MS and LC-HRMS tests on Moringa oleifera Seed Oil (MOSEIL) and Moringa oleifera Leaves Powder (MOLP) which are oil and flour products made from moringa. The results of the identification of phytochemical compounds through the GC-MS test showed that the dominant compound in MOSEIL was oleic acid (37.546%) and in MOLP was ester (8.802%) when using n-hexane as solvent. The percentage yield of the dominant compound from the LC-HRMS test in MOSEIL was nitro compound (72.55%) and at MOLP was alcohol (45.87%). These compounds are known to have effects as hepatoprotective agents through antioxidant, anti-inflammatory, and anti-fibrotic activities that can reduce hepatic oxidative stress as an early trigger of cancer development. Through molecular docking, MOSEIL and MOLP showed a lower level of binding affinity when compared to TGF-β1 control drugs such as metformin. This data implies MOSEIL and MOLP have a strong interaction to TGF-β1 than the control drug. The therapeutic potential of the hepatoprotective properties of MOSEIL and MOLP makes them one of the most-promising therapeutic agents in the initial step of renewable cancer treatment therapy.

Effects of Moringa oleifera Seed Oil on Cultured Human Sebocytes In Vitro and Comparison with Other Oil Types.

The seeds of Moringa oleifera (horseradish tree) contain about 40% of one of the most stable vegetable oils (Moringa seed oil). Therefore, the effects of Moringa seed oil on human SZ95 sebocytes were investigated and were compared with other vegetable oils. Immortalized human SZ95 sebocytes were treated with Moringa seed oil, olive oil, sunflower oil, linoleic acid and oleic acid. Lipid droplets were visualized by Nile Red fluorescence, cytokine secretion via cytokine antibody array, cell viability with calcein-AM fluorescence, cell proliferation by real-time cell analysis, and fatty acids were determined by gas chromatography. Statistical analysis was performed by the Wilcoxon matched-pairs signed-rank test, the Kruskal-Wallis test and Dunn's multiple comparison test. The vegetable oils tested stimulated sebaceous lipogenesis in a concentration-dependent manner. The pattern of lipogenesis induced by Moringa seed oil and olive oil was comparable to lipogenesis stimulated by oleic acid with also similar fatty acid secretion and cell proliferation patterns. Sunflower oil induced the strongest lipogenesis among the tested oils and fatty acids. There were also differences in cytokine secretion, induced by treatment with different oils. Moringa seed oil and olive oil, but not sunflower oil, reduced the secretion of pro-inflammatory cytokines, in comparison to untreated cells, and exhibited a low n-6/n-3 index. The anti-inflammatory oleic acid detected in Moringa seed oil probably contributed to its low levels of pro-inflammatory cytokine secretion and induction of cell death. In conclusion, Moringa seed oil seems to concentrate several desired oil properties on sebocytes, such as high content level of the anti-inflammatory fatty acid oleic acid, induction of similar cell proliferation and lipogenesis patterns compared with oleic acid, lipogenesis with a low n-6/n-3 index and inhibition of secretion of pro-inflammatory cytokines. These properties characterize Moringa seed oil as an interesting nutrient and a promising ingredient in skin care products.

Study progress on moringa seed oil with microemulsion: bibliometrics analysis of year 2012-2023

Transdermal drug delivery systems increase the effectiveness of a drug substance, and one of them is a microemulsion. Moringa oil has anti-inflammatory, antioxidant, and antibacterial activity. The purpose of this study is to conduct a bibliometric analysis research on moringa oil formulated as microemulsions. A total of 5 English articles from the Scopus databases from 2012 to 2023 US sources were used in the bibliometrics analysis of Moringa oleifera seeds oil related to microemulsions. The bibliometrics analysis shows research trends, including the most used keywords depicted in visualizations using the VOSViewer application, article publishers, affiliations, and the author's country of origin. The most used keywords are microemulsions, microemulsion, Moringa oleifera, antioxidant activities, and antioxidants. The most publishers are Key Engineering Materials, the most affiliated are Chiang Mai University and Thamasat University, and most authors are from Thailand. The study of microemulsion formulations of Moringa seed oil has yet to be widely carried out but has been cited by other researchers.

Hepatoprotective Effects of Moringa Oleifera Seed Oil and Pumpkin Seed Oil on CCL4 Induced Hepatotoxicity in Rats

Hepatoprotective Effects of Moringa Oleifera Seed Oil and Pumpkin Seed Oil on CCL4 Induced Hepatotoxicity in Rats

Evaluation of the Therapeutic Effects of Moringa oleifera Seed Oil on Cadmium and Herbal Alcoholic Beverage–Induced Prefrontal Cortex Damage in Wistar Rats

The use of Moringa oleifera seed oil in the prevention of neurodegenerative diseases is on an increasing trend. Cadmium is one of the most toxic environmental pollutants causing many known damage to the brain, the consumption of herbal alcoholic beverages is known to cause neurodegeneration. The aim of this study was to investigate the ameliorative effects of Moringa oleifera seed oil on cadmium and herbal alcoholic beverage induced- damage to the frontal cortex of Wistar rats. Eighty Wistar rats were divided into eight groups of 10 rats each. Group A served as a control which received 2.5mg/kgbw phosphate buffer intra-peritoneally, while group F served as Moringa-treated control and received oral administration of 2.0 mg/kgbw Moringa oleifera oil. Groups B1, B2, D and E were injected intra-peritoneally with 3.5mg/kgbw CdSO4.8H2O single dose. Group C1, C2 and D received oral administration of 0.5 ml Herbal Alcoholic Beverage (HAB) and group B2, C2and E were administered orally with 2.0mg/kgbw Moringa oleifera oil for four weeks followed by sacrificed. Quantitative enzymes and biochemical antioxidant markers showed that cadmium and HAB administration caused a significant increase in AchE, SDH, CAT, GPx and MDA levels and decrease in SOD level. Conversely, there were significant decrease in AchE, SDH, CAT, GPx and MDA and increased SOD level upon administration of Moringa oleifera oil. Moringa oleifera seed oil has natural antioxidant constituents that ameliorated damage caused by Cd and HAB.

Triacylglycerol characterisation of Moringa oleifera seed oil by LC‐Q‐TOF and ESI

SummaryCurrently, in the analysis of triacylglycerol (TAG) composition of the Moringa oleifera seed oil (MOSO), there are still approximately 13.9% of TAG still unknown. Reverse‐phase high‐performance liquid chromatography (HPLC) coupled to a refractive index detector (RID) has previously been applied for the identification of TAG in MOSO. A new approach focuses on the properties of the fatty acid assay and the full characterisation of TAGs. Identification and determination of TAG compositions in MOSO were done by LC‐Q‐TOF (LCMS‐9030) with ESI interface and HCOONH4 (ammonium formate) additive in the mobile aqueous phase. Triolein (OOO) was 39.3% and is the dominant TAG in MOSO. This new method was able to fully characterise a total of 33 TAG compositions using accurate mass MS data and MS/MS spectra.

Formulation and Evaluation of Liposome Moringa Oleifera Seed Oil (Moringa oleifera L.) as Anti-aging

Moringa oil is a natural vegetable oil obtained from the juice of the ripe kernels of the Moringa tree (Moringa oleifera L). Moringa seeds have an oil content of up to 40%, and have antioxidant activity with vitamin E content reaching 51% but have low stability. The purpose of this study was to find the best formulation as an anti-aging on liposome delivery system with a ratio of phosphatidylcholine soy lechitin and cholesterol. The method used is thin layer hydration (Thin Film Hydration). The results of the observation on the best formula are the 6th formula on organoleptic examination which is milky yellow in color, has a distinctive smell of lecithin and cholesterol and is in the form of a thick suspension, with a pH of 6.0, particle size 83.98nm using a particle size analyzer and entrapment efficiency of 96.8 % using a UV-Vis spectrophotometer.

Hepatoprotective and Renoprotective effect of Moringa oleifera Seed Oil on Dichlorvos-induced Toxicity in Male Wistar rats.

The liver and the kidney are one of the vital organs of body. Drug-induced toxicity is one of the most common problems encountered by these organs. The search for an effective medicine to treat this toxicity without any side effects has led to the use of traditional-based medicine. This study evaluated the effect of ethanolic extract of Moringa oleifera seed oil on hepatic and renal markers in dimethyl 2, 2-dichlorovinyl phosphate (DDVP, known as dichlorvos)-exposed Wistar rats. Twenty-one male Wistar rats were randomly divided into three groups of seven animals each. Group A served as the negative control and were not exposed to dichlorvos. Group B served as the positive control and were exposed to dichlorvos for 2 minutes but received no extract. Group C animals were exposed to the dichlorvos and received 300mg/kg of extract (Moringa oleifera seed oil) for 7days before and 21days after exposure. Exposure to DDVP led to a significant increase in hepatic & renal markers, inflammatory markers, decrease in plasma protein and alteration of plasma electrolyte. Moringa oleifera seed oil regulated and significantly enhanced plasma protein, reduced elevated levels of hepatic & renal markers, inflammatory markers in the study sample. In addition, histopathology observation showed that Moringa seed oil was able to regenerate the hepatorenal damage on exposure to dichlorvos. Conclusion: Moringa oleifera seed oil exhibited hepato-protective, nephroprotective properties and could be explored in nutrition and health.

Optimization of Supercritical CO2 Extraction of Moringa oleifera Seed Oil Using Response Surface Methodological Approach and Its Antioxidant Activity

ObjectivesMoringa (Moringa oleifera), also known as the Drumstick tree, is a Moringa plant found in the Moringa family. Moringa is rich in multiple biological aspects such as, protein, amino acids, fibers, vitamins, and essential minerals, including potassium, calcium, iron, and zinc. In addition, Moringa seeds contain a relatively large amount of oil, which has been shown to benefit human health. Moringa seed oil contains a high quantity of unsaturated fatty acids than most common edible oils. Supercritical CO2 fluid extraction is particularly suitable for the separation of physiologically active substances and natural products, including plant seed oil. Thus, the present study aimed to determine the optimal process conditions of supercritical CO2 fluid extraction of moringa seed oil.MethodsWe employed supercritical CO2 fluid extraction as well as the response surface test design on the basis of single-factor experiment to investigate the three factors of extraction, temperature, time, and pressure, on the extraction rate of Moringa seed oil.ResultsThe optimal process conditions were determined as extraction temperature of 45°C, extraction time of 2.5 h, extraction pressure of 50 MPa, and CO2 flow rate of 240 L/h, resulting in a maximum yield of 38.54%. This resulting maximum yield is higher than prior extraction methods of moringa seed oil. Composition analysis shows that the extracted moringa seed oil is rich in unsaturated fatty acids, including oleic acid, octadecanoic acid, palmitic acid, stearic acid, eicosanoic acid, etc. Furthermore, we found that moringa seed oil exerted potent antioxidant activities on DPPH and hydroxyl radicals, and its efficacy was comparable to commercial peanut oil and tea oil.ConclusionsThis study provides a scientific basis for the development and application of Moringa seed oil in functional food, nutraceutical, and cosmetic products.Funding SourcesThis work was supported by General project of Basic and applied basic Research in Guangzhou (2,060,206); Natural Science Foundation of Guangdong basic and applied basic research foundation (2021A1515010965); Natural Science Foundation of Guangdong basic and applied basic research foundation ([2018]105), Laboratory opening project of Guangzhou Medical University (PX-1,020,423).

Optimization of Supercritical CO2 Extraction of Moringa oleifera Seed Oil Using Response Surface Methodological Approach and Its Antioxidant Activity.

Moringa (Moringa oleifera) seed oil is an edible vegetable oil rich in unsaturated fatty acids. In this study, the supercritical CO2 fluid extraction method was employed to obtain the maximum yield of moringa seed oil. The effects of temperature, time, and pressure, three characteristics of extractions, on the extraction rate of Moringa seed oil were investigated by single factor test and response surface methodological approach. The optimal process conditions of supercritical CO2 fluid extraction of moringa seed oil were determined as extraction temperature of 45°C, extraction time of 2.5 h, extraction pressure of 50 MPa, and CO2 flow rate of 240 L/h, resulting in a maximum yield of 38.54%. Composition analysis shows that the extracted moringa seed oil is rich in unsaturated fatty acids, including oleic acid, octadecanoic acid, palmitic acid, stearic acid, eicosanoic acid, etc. Furthermore, we found that Moringa seed oil exerted potent antioxidant activities on DPPH and hydroxyl radicals, and its efficacy was comparable to commercial peanut oil and tea oil. Overall, this novel extraction method of moringa seed oil may increase its potential value and application in the food and nutraceutical industries.

Composition and Characterization of Cold Pressed Moringa oleifera Seed Oil.

The present study aims to investigate the volatile compound and the triacylglycerol profiles of Tunisian cold pressed Moringa oleifera seed oil (MoSO) and to assess its thermal properties and its biological activities. GC-MS analysis identified thirty six phyto-compounds amounting to 98.99% of the total oil. These compounds were classified into eleven groups among which the fatty acid one exhibited the highest intensity (91.63%). Cis, 6-octadecenoic acid was the most abundant compound (70.68%). The triacylglycerol composition of MoSO was characterized by the predominance of the glycerol trioleate (OOO) (32.42±0.12%). Thermogravimetric analysis of MoSO showed that the oil possess an interesting thermal stability with a highly Onset temperatures (Tonset) of 390.72°C and 357.47°C, respectively in nitrogen and air atmospheres. By using the ABTS assay, MoSO exhibited an interesting antioxidant capacity of 365 μM TEAC. The oil was also endowed with a relatively strong anti-inflammatory activity since its treatment at the different concentrations tested (75, 150 and 300 μg/mL). However, no antimicrobial activity was observed. On the basis of the obtained results, MoSO could be used in diverse industrial applications such as pharmaceutical, cosmetic, and food fields thanks to its thermal stability and interesting biological activities.

Ultrasound-assisted Extraction and Characterization of Mexican Moringa oleifera Seed Oil: Identification of Tocopherols and Phytosterols Using UPLC-APCI+MS/MS

Ultrasound-assisted Extraction and Characterization of Mexican Moringa oleifera Seed Oil: Identification of Tocopherols and Phytosterols Using UPLC-APCI+MS/MS

Moringa oleifera seed oil extracted by pressurized n-propane and its effect against Staphylococcus aureus biofilms

ABSTRACT Staphylococcus aureus is often associated worldwide with foodborne illnesses, and the elimination of biofilms formed by this bacterium from industrial surfaces is very challenging. To date, there have been few attempts to investigate plant oils obtained by recent green technologies, applied against biofilms on usual surfaces of the food industry and bacteria isolated from such environment. Therefore, this study evaluated the activity of Moringa oleifera seed oil (MOSO), extracted with pressurized n-propane, against standard and environmental S. aureus biofilms. Additionally, a genotypic and phenotypic study of the environmental S. aureus was proposed. It was found that this bacterium was a MSSA (methicillin-sensitive S. aureus), a carrier of icaA and icaD genes that has strong adhesion (OD550=1.86 ± 0.19) during biofilm formation. The use of pressurized n-propane as a solvent was efficient in obtaining MOSO, achieving a yield of 60.9%. Gas chromatography analyses revealed the presence of a rich source of fatty acids in MOSO, mainly oleic acid (62.47%), behenic acid (10.5%) and palmitic acid (7.32%). On polystyrene surface, MOSO at 0.5% and 1% showed inhibitory and bactericidal activity, respectively, against S. aureus biofilms. MOSO at 1% allowed a maximum reduction of 2.38 log UFC/cm² of S. aureus biofilms formed on PVC (polyvinyl chloride) surface. Scanning electron microscopy showed disturbances on the surface of S. aureus after exposure to MOSO. These unprecedented findings suggest that MOSO extracted with pressurized n-propane is potentially capable of inhibiting biofilms of different S. aureus strains, thus, contributing to microbiological safety during food processing.