Abstract
SummaryCurrently, in the analysis of triacylglycerol (TAG) composition of the Moringa oleifera seed oil (MOSO), there are still approximately 13.9% of TAG still unknown. Reverse‐phase high‐performance liquid chromatography (HPLC) coupled to a refractive index detector (RID) has previously been applied for the identification of TAG in MOSO. A new approach focuses on the properties of the fatty acid assay and the full characterisation of TAGs. Identification and determination of TAG compositions in MOSO were done by LC‐Q‐TOF (LCMS‐9030) with ESI interface and HCOONH4 (ammonium formate) additive in the mobile aqueous phase. Triolein (OOO) was 39.3% and is the dominant TAG in MOSO. This new method was able to fully characterise a total of 33 TAG compositions using accurate mass MS data and MS/MS spectra.
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