Moringa Oleifera Leaves Extract Research Articles

Preparation and Evaluation of Oleogels Incorporated with Moringa oleifera Leaves Extract in Biscuits Production

Preparation and Evaluation of Oleogels Incorporated with Moringa oleifera Leaves Extract in Biscuits Production

Evaluation of the Effects of Whey Protein and Moringa Oleifera Leaves Extract Mixture on Osseointegration in Rabbits.

Osteogenic, antioxidant and anti-inflammatory effects of Whey protein and M. oleifera gel prompted us to evaluate their role alone or in combination on osseointegration in rabbits. In this study, 24 titanium implants were inserted in the femurs of six rabbits. One implant was placed without treatment, and another one was coated with a mixture of whey protein and M. oleifera gel for each side. The animals were divided into two groups of 2- and 6-week intervals and evaluated using histopathological and immunohistochemical techniques. Histological evaluation revealed a significant difference between the experimental and the control groups after two weeks in osteoblast and osteocyte counts. The experimental group had mature bone development after six weeks of implantation, while the control group had a woven bone. Immunohistochemical results showed that the experimental group, compared to the control group, exhibited early positive expression of osteoblast cells at two weeks after the experiment. Based on histopathological observations, the experimental group showed a tiny area of collagenous fiber in 6th week after the implantation. A mixture of whey protein and M. oleifera could accelerate osseointegration and healing processes.

Formulation and Evaluation of Anti-inflammatory Ointment Containing Moringa oleifera Leaves Extract

Formulation and Evaluation of Anti-inflammatory Ointment Containing Moringa oleifera Leaves Extract

Formulation and Antioxidant Activity Test of Centella asiatica Herba Extract and Moringa oleifera Leaves Extract as An Anti-Aging Emulgel

Formulation and Antioxidant Activity Test of Centella asiatica Herba Extract and Moringa oleifera Leaves Extract as An Anti-Aging Emulgel

Chemoprevention of DMH Induced Colon Carcinogenesis by Combination Administration of Lactobacillus acidophilus, Calcium and Moringa oleifera Leaves Extract

Colon cancer has become one of the most prevalent and deadliest type of cancer in the globe in recent years. In order to prevent colon cancer triggered by DMH (1,2-dimethyl hydrazine hydrochloride), this study was to investigate the potential chemopreventive effects of calcium citrate, Lactobacillus acidophilus, and Moringa oleifera leaf extract. Sprague Dawley rats were randomized into 10 different groups and received subcutaneous injections of DMH (30 mg/kg) for 8 weeks of duration. Treatment groups were served for 4 weeks, 5-Flurouracil (20 mg/kg) was dosed with two intraperitoneal injections in a week and. L. acidophilus (1 × 109 cfu/kg p.o.), calcium citrate (2 mg/kg p.o.) and hydroalcoholic extract of M. oleifera leaves (100 and 200 mg/kg p.o.) were used for daily. All animals were killed after the completion of treatment, and colonic tissues were assessed for biochemical and histopathological parameters. DMH treatment-induced cellular and systemic oxidative stress results in depletion of cellular antioxidant enzyme levels with an alteration in hematological counts. The colonic tissues had a loose histoarchitecture with severe cellular infiltration, edema in the epithelium, and a moderate nuclear/cytoplasmic ratio, goblet cells. Combination treatments of L. acidophilus (LA), Calcium citrate (CC), and hydroalcoholic extract of M. oleifera leaves (ME) showed positive improvements in antioxidant enzyme levels, hematology count, and colonic tissue histoarchitecture. From outcomes of the present work concluded that a combination treatment of L. acidophilus, calcium citrate and a hydroalcoholic extract of M. oleifera leaves showed chemo-preventive effects against colon cancer triggered by DMH.

Assessment of the hepatoprotective effect of Moringa oleifera leaves ex-tract (MOLE) on paracetamol-induced hepatotoxicity in Albino Wistar rats

The current study aimed to investigate the hepatoprotective efficacy of Moringa oleifera (MOLE) against liver damage in male rats caused by paracetamol. Four groups of twenty-four male Wister rats, weighing between 150 and 200 gm each, were created. To cause liver damage, a dose of 2g/kg of paracetamol was given once a day for a week, and a dose of 300mg/kg B.W. of MOLE was given orally via gavage for four weeks. As an example hepatoprotective medication, silymarin was administered orally at a rate of 100 mg/kg body weight. Enzyme levels in the liver including total bilirubin, ALT, AST, and ALP were utilized to gauge the extent of recovery after hepatic damage. As antioxidant enzymes, SOD and CAT were evaluated. In male rats, paracetamol-induced liver damage was successfully avoided by silymarin and MOLE. The hepatoprotective effects of the extract were evidenced by a noteworthy reduction in the levels of blood enzymes include TB, ALT, AST, and ALP, along with a decline in tumour necrosis factor alpha (TNF-α). Additionally, there was a rise in the levels of antioxidant enzymes SOD and CAT, as well as interleukin 6 (IL-6).

Exploring the Influence of Moringa oleifera Leaves Extract on the Shelf Life of Ground Beef during Refrigerated Storage

Exploring the Influence of Moringa oleifera Leaves Extract on the Shelf Life of Ground Beef during Refrigerated Storage

Dentinomimetics and cementomimetics of Moringa oleifera leaves extract

To evaluate the biomimetic remineralization capabilities of Moringa oleifera leaves (MOL) extract on coronal dentin and acellular cementum, two different concentrations (50 and 200 mg/ml) of MOL extract loaded in plain varnish (M1 and M2 groups respectively) were compared to fluoride varnish (FL group) and native surface (C group). Eighty sound premolar teeth were collected. Forty teeth (10 teeth in each group) were used for coronal dentin testing while the other forty (10 teeth in each group) were used for acellular cementum testing. Teeth in M1, M2, and FL groups were etched for 30 s and then received the specific varnish treatment. All samples were immersed in artificial saliva for 14 days and then collected, dried, and examined by scanning electron microscopy and energy dispersive X-ray spectroscopy (EDX). Histologically, FL group showed mineral deposition as discrete vesicular granules of various sizes on the surface of both coronal dentin and acellular cementum. Mineral deposition only occurred on some DTs openings while opened tubules remained. The surface of the acellular cementum revealed regular grooves, micro-fissures, and cracks. In the M1 and M2 groups, mineral deposition appeared as a homogenous continuous layer on coronal dentin and acellular cementum. Only a few DTs and cementum fissures were not filled completely. In L.S. sections of the coronal one-third, the DTs appeared almost sealed with varying lengths of mineral deposition. EDX results statistical analysis showed that the M2 group had the highest phosphate ions (P−) and calcium ions (Ca+2) at%. MOL has an extraordinary effect on the remineralization of coronal dentin and acellular cementum. It would have a promising ability to control dentinal hypersensitivity and formation of biomimetic cementum tissue.

Effect of Moringa oleifera Leaves Extract, Whey Protein, and Their Combination on Growth, Carcass and Meat Quality of Broiler Chickens

Effect of Moringa oleifera Leaves Extract, Whey Protein, and Their Combination on Growth, Carcass and Meat Quality of Broiler Chickens

Antimicrobial Effect of Moringa oleifera Leaves Extract on Foodborne Pathogens in Ground Beef.

Consumers nowadays are becoming more aware of the importance of using only meat products containing safe and natural additives. Hence, using natural food additives for extending the shelf life of meat along with delaying microbial growth has become an urgent issue. Given the increasingly popular view of Moringa oleifera leaves as a traditional remedy and also the scarcity of published data concerning its antimicrobial effect against foodborne pathogens in meat and meat products, we designed the present study to investigate the antimicrobial effect of Moringa oleifera leaves aqueous extract (0.5%, 1%, and 2%) on ground beef during refrigerated storage at 4 °C for 18 days. MLE revealed potent antimicrobial properties against spoilage bacteria, such as aerobic plate count and Enterobacteriaceae count. MLE 2% showed a significant (p < 0.01) reduction in the counts of E. coli O157:H7, Salmonella enterica serovar Typhimurium, and Staphylococcus aureus artificially inoculated to ground beef by 6.54, 5.35, and 5.40 log10 CFU/g, respectively, compared to control, by the 18th day of storage. Moringa leaves extract (MLE) had no adverse effect on the overall acceptability and other sensory attributes; moreover, it induced a slight improvement in the tenderness and juiciness of treated ground beef, compared to the control. Therefore, MLE can be used as a healthy, natural, and safe preservative to increase meat products' safety, quality, and shelf stability during cold storage. A promising approach for using natural food additives rather than chemical preservatives could begin new frontiers in the food industry, as they are more safe and do not constitute health risks to consumers.

Drug-Herb Interactions: Selected Antihypertensive Drugs with Moringa oleifera Leaves Extract

Moringa oleifera is a local plant which is commonly used in cooking and as a health supplement. It has been reported to possess blood pressure (BP) lowering effect and little is known about its possible interactions with cardiovascular drugs. This study looked into the possible drug-herb interactions between M. oleifera leaves extract and selected antihypertensive drugs. Ten groups of spontaneously hypertensive rats (SHRs) and one group of normotensive rats (NTs) were administered either M. oleifera extract alone, drugs alone or drugs in combination with M. oleifera extract for 14 days and BP of the rats were measured. Angiotensin converting enzyme (ACE) activity was also determined in vitro and ex vivo. Treated groups were found to produce significant BP reduction on day 15 when compared with the negative control but there was no significance difference when compared with positive controls (drugs alone). M. oleifera extract administered alone significantly reduced BP of SHRs on day 15 and this is comparable with the BP reduction observed when antihypertensive drugs were administered alone. However, no additive effect was observed when drugs were used in combination with M. oleifera extract. Similar results were seen in the in vitro and ex vivo ACE inhibitory activity of M. oleifera extract and enalapril. It can be concluded that there is a possibility of drug-herb interaction between M. oleifera extract and the selected antihypertensive drugs.

English

The prospects for using&nbsp;Moringa oleifera&nbsp;leaves extract (MLE) supplemented with different concentrations of kanwa or sodium bicarbonate (NaHCO3) as a low-cost alternative growth medium of&nbsp;Spirulina&nbsp;platensis&nbsp;were evaluated in a small-scale outdoor cultivation system. The present study was aimed to evaluate the potential of MLE growth medium enriched with different concentrations (4 or 8 g L-1) of kanwa and NaHCO3&nbsp;on growth, chlorophyll content, biochemical characteristics, antioxidant compounds and some physico-chemical factors. Jourdan&rsquo;s standard medium was taken as control. The results showed that the growth parameters such as cell population, biomass dry weight, cell productivity and specific growth rate were positively affected in MLE cultivation medium enriched with kanwa or NaHCO3&nbsp;at different concentration levels. The addition of urea, kanwa or NaHCO3&nbsp;in MLE cultivation medium at different concentration levels increased significantly (p&lt; 0.05) the protein content, the peroxidase and polyphenol oxidase activity, the conductivity, pH, total dissolved solids and salinity from 20 to 25 days of cultivation whereas a decrease in carbohydrate and phenol content was recorded during all the period of the experimentation. The highest values of growth parameters were notably in MLE medium supplemented with urea and kanwa at 8 g L-1. The MLE medium enriched with urea and kanwa at 8 g L-1&nbsp;was shown to be an appropriate growth medium that can be used as a low-cost alternative growth medium for commercial cultivation of&nbsp;S. platensis. &nbsp; Key words:&nbsp;Antioxidant compounds, biochemical traits, growth,&nbsp;Moringa oleifera&nbsp;leaves extract, physico-chemical factors,&nbsp;Spirulina platensis.

Bioremediation of Vanki River Water &amp; Industrial Effluents using Calotropis procera Latex Exudate and its Comparative Analysis against Moringa oleifera Leaves Extract

This study was carried out to check the effectiveness of Calotropis procera latex for bioremediation and to compare its efficacy with Moringa oleifera leaves extract. It was observed that considerable reduction in various physicochemical and bacteriological properties of the samples took place with Calotropis procera latex. After 1 hour of treatment itself, it carried out pH reduction of 11.11%, 30.00% and 12.50%, while Moringa oleifera leaves extract carried out 0.00%, 20.00% and 0.00% reduction and after 24 hours, turbidity was reduced to 90.00%, 61.87% and 65.36% with Calotropis procera latex and 75.00%, 60.43% and 32.03% with Moringa oleifera leaves extract for Vanki river water, Textile effluent and Paper &amp; Pulp Industrial effluent, respectively. Vanki river water showed a reduction in TSS and TDS of 2000 mg/L and 34000 mg/L with Calotropis procera latex and 3000 mg/L and 46000 mg/L, respectively with Moringa oleifera leaves extract. With Calotropis procera latex, the COD and BOD values were 544 mg/L, 608 mg/L and 800 mg/L; and 0.04 mg/L, 0.240 mg/L and 0.012 mg/L for Vanki river water, Textile effluent and Paper &amp; Pulp Industrial effluent, respectively. When treated with Moringa oleifera leaves extract, the COD values reduced to 864 mg/L, 800 mg/L and 896 mg/L and BOD values reduced to 0.004 mg/L, 0.251 mg/L and 0.022 mg/L, respectively. The total coliform count reduced to 130 MPN/100 ml, &lt;1.8 MPN/100 ml and 7.8 MPN/100 ml for Vanki river water, Textile effluent and Paper &amp; Pulp Industrial effluent, respectively after treatment with Calotropis procera latex. Thus, Calotropis procera showed better results in comparison with Moringa oleifera. So, it could be considered as the best alternative to prevent pollution.

Cypermethrin-Induced Lung Damage in Albino Rats: The Preventive Impact of Moringa oleifera

Cypermethrin (CYP) is a popular environmental toxicant because of its wide-ranging use as a broad-band insecticide. The purpose of this research was to explore the potential impact of Moringa oleifera leaves extract (MOLE) as a protective agent against cypermethrin-induced lung toxicity in male albino rats. Forty experimental animals were allocated into four groups. G1 served as the negative control, G2, G3 and G4 were orally treated with MOLE (250 mg/kg body weight), CYP (26.15mg/kg body weight) and CYP + MOLE, respectively. CYP oral administration for 28 days elevated the levels of oxidative stress biomarkers like Malondialdehyde, superoxide dismutase, catalase and glutathione S-transferase and caused a decline in the content of reduced glutathione in the lung tissue of the rats. Lung injury was verified by histopathological changes evidenced by interstitial pneumonia, marked perivasculitis, focal pulmonary hemorrhage, haemosidrosis and pulmonary edema in CYP-exposed rats. Co-administration of MOLE mitigated the induced oxidative stress and histopathological alterations. As a result, Moringa oleifera can prevent cypermethrin-induced lung toxicity due to its free radical scavenging and antioxidant properties.

Neuroprotective Assessment of Moringa oleifera Leaves Extract against Oxidative-Stress-Induced Cytotoxicity in SHSY5Y Neuroblastoma Cells.

The current trend worldwide is searching plant extracts towards prevention of neurodegenerative disorders. This study aimed to investigate the neuroprotective effect of Alpinia galanga leaves (ALE), Alpinia galanga rhizomes (ARE), Vitis vinifera seeds (VSE), Moringa oleifera leaves (MLE), Panax ginseng leaves (PLE) and Panax ginseng rhizomes (PRE) ethanolic extracts on human neuroblastoma (SHSY5Y) cells. The 1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging of VSE and MLE were 81% and 58%, respectively. Ferric-reducing antioxidant power (FRAP) of ALE and MLE (33.57 ± 0.20 and 26.76 ± 0.30 μmol Fe(ΙΙ)/g dry wt., respectively) were higher than for the other extracts. Liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC-QTOF/MS) revealed MLE active compounds. Intracellular study by nitro-blue tetrazolium (NBT) test showed that MLE and VSE had high O2− scavenging (0.83 ± 0.09 vs. 0.98 ± 0.08 mg/mL, respectively). MLE had the highest ROS scavenging followed by PRE (0.71 ± 0.08 vs. 0.83 ± 0.08 mg/mL, respectively), by 2,7-dichlorodihydrofluorescein diacetate (DCFHDA) assay. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity and neuroprotection tests on SHSY5Y showed that PRE had a better neuroprotective effect but higher cytotoxicity compared to MLE (viable cells 51% vs. 44%, IC50 1.92 ± 0.04 vs. 2.7 ± 0.2 mg/mL, respectively). In conclusion, among the studied plants, MLE has potential for developing as a neuroprotective agent.

Enhancement impact of Moringa oleifera leaves extract–base extender on cryopreservation and fertilization of Barki ram sperms: comparative study with vitamin E and selenium combination

This study evaluated the impacts of inclusion of Moringa Oleifera leaves extract (MOLE) in semen extender on rams cryopreserved semen quality and fertilization potential. Forty ejaculates were collected from eight fertile Barki rams, pooled and divided into five groups. The semen extender of the control group was without additives. The semen extender of the second and third groups was supplemented with MOLE at doses of 300 and 600 µg/mL, respectively. The semen extender of the fourth and fifth groups was supplemented with a combination of vitamin E and selenium at doses of 2.5 and 5 µg/mL, respectively. One hundred ten multiparous Barki ewes were artificially inseminated with the semen supplemented without or with MOLE or vitamin E and selenium combination. Inclusion of MOLE in semen extender significantly elevated the motility, viability index, membrane integrity and fertilization capacity of the post-thawed spermatozoa, as well as the activities of semen catalase, total antioxidant capacity, superoxide dismutase, glutathione peroxidase, and glutathione reductase. However, it significantly decreased acrosomal defects and DNA fragmentation of spermatozoa, the activities of semen alkaline and acid phosphatase and the concentration of malondialdehyde compared with the other groups. Similarly, vitamin E and selenium significantly improved the above-mentioned parameters compared to those of the control group. This study indicated that inclusion of MOLE to semen extender improved the quality and fertility of the post-thawed rams’ semen through enhancing the activities of the antioxidant enzyme system and decreasing the spermatozoa DNA fragmentation and lipid peroxidation. Highlights Moringa Oleifera leaves extract (MOLE) protected spermatozoa against cryopreservation induced oxidative stress. MOLE enhanced cryopreserved semen quality. MOLE enhanced post-thawed spermatozoa fertilization capacity.

Exploring the influence of Moringa oleifera leaves extract on the nutritional properties and shelf life of mutton patties during refrigerated storage

ABSTRACT The influence of Moringa oleifera leaves extract (MOLE) on the nutritional properties, thiobarbituric acid reactive substances, microbial composition and physicochemical characteristics of mutton patties during refrigerated storage was explored. The mutton patties were processed by incorporating different amounts (1, 2, 3 and 5%) of MOLE except for the control sample. The inclusion of MOLE significantly increased (p < .05) the protein (21.75%), ash (2.73%), total phenolic (41.96 mg GAE/g) and total flavonoids (20.93 mg CE/g) contents of mutton patties while moisture and fat contents decreased during storage. Lipid oxidation and microbial growth significantly increased while pH values of raw patties decreased. The lightness (L*), redness (a*), yellowness (b*) and chroma (C*) values significantly decreased; while hue angle (H°) value increased. The inclusion of MOLE exhibited cooking yield and moisture retention values of 66.68 and 67.32, respectively. The study results show that MOLE can be utilised as natural antioxidant and preservative of mutton patties.

The Protective Effect of Moringa Oleifera Leaves Extract on Intestinal Injury Caused by Amethopterin Administration in Mice

The Protective Effect of Moringa Oleifera Leaves Extract on Intestinal Injury Caused by Amethopterin Administration in Mice

Screening the Antibacterial Activity of Moringa oleifera Leaves and Seeds Extract against Selected Members of Bacteria

Screening the Antibacterial Activity of Moringa oleifera Leaves and Seeds Extract against Selected Members of Bacteria

Assessment of Buffalo Semen Preservability Using Tris Extender Enriched With Moringa Oleifera Extract

The aim of the present study was to explore the effect of Moringa oleifera leaves extract (MLE) on buffalo spermatozoa during cooling and cryopreservation. Pooled bull semen were diluted by Tris-Citrate-Fructose (TCF) diluent supplemented with MLE and allocated in 6 concentrations which were 0% control, 10, 20, 30, 40, 50% (v/v) [MLE: TCF] then 20% egg yolk was added, mixed and finally stored at -20°C until used. Diluted semen was cooled slowly up to 5 °C and equilibrated for 4 h. Semen was packed into 0.25 mL polyvinyl straws. After equilibration periods, the straws were exposed to liquid nitrogen (LN2) vapor for 10 minutes and were then dipped in LN2. Extended semen was subjected to evaluation (motility, alive %, abnormality %, and intact sperm membrane (HOST) %) in both chilled and frozen semen. Sperm motility was significantly (P<0.0001) kept high after 11 days of chilling with the concentration 10 and 20%, also it was kept significantly (P<0.0001) high with the concentration 30 and 40% up to 10 days. The use of MEEY had significantly (P<0.0001) improved sperm motility% with the concentration 10 and 20% compared to the control, while the other concentrations didn’t exhibit any effect. On the other hand, MEEY had maintained alive sperm, intact spermatozoa membrane% and abnormal sperm % as good as the control with all the concentrations of MEE used in the present study. Then, it could be concluded that 10-20% of Moringa oleifera leaves extract (under the contemporary conditions of extraction in this study) as a natural additive to semen extenders improved preservability in both chilled and frozen bull semen.