Moringa Oleifera Leaf Extract Research Articles

Phytochemical Screening and Antibacterial Activity of Moringaoleifera Leaf Extract Western Libya

Phytochemical Screening and Antibacterial Activity of Moringaoleifera Leaf Extract Western Libya

Effect of moringa oleifera with pure and Zn-doped Mn and Ni nanoferrites for hyperthermia applications

Nanoferrites like spinel manganese ferrite (MnFe2O4) and inverse spinel nickel ferrite (NiFe2O4) have an efficient heating deportment to the destruction of neoplasm. The incorporation of Moringa oleifera (MO) leaf extracts with magnetic nanoparticles as heat mediators is an effective approach for treating magnetic hyperthermia. This study investigated the potential heating behavior of bare, Zn-doped MnFe2O4 and NiFe2O4 nanoparticles (NPs) with Moringa oleifera leaf extracts as magnetic hyperthermia agents. High-resolution transmission electron microscopy showed the spherical-like morphology of MnFe2O4 NPs with no impurities in the EDX spectra. X-ray diffraction spectra confirmed the cubic spinel and inverse spinel structure for MnFe2O4 and NiFe2O4 NPs, respectively. Magnetic properties of the prepared nanoferrites exhibit low coercivity values (in the range of 13–18 Oe representing superparamagnetic behavior. Further, the binding energies of the prepared nanoferrites were revealed by X-ray photoelectron spectroscopy. The effective magnetic anisotropy constant (Keff) was calculated to be around 3.9–5.9 kJ/m3 by electron spin resonance. An increased specific surface area (120.69 m2/g) and wider porosity (17.053 nm) were observed for MnFe2O4/MO NPs through Brunauer-Emmett-Teller (BET) analysis. Thermal properties were investigated by measuring the effective specific absorption rate in an alternating magnetic field ranging from 23 to 31 * 10−9 W/g Oe2 Hz. Results indicated that the green synthesized MnFe2O4/MO NPs are promising agents for magnetic hyperthermia applications in cancer therapy.

Synthesized Gold Nanoparticles with Moringa Oleifera leaf Extract Induce Mitotic Arrest (G2/M phase) and Apoptosis in Dalton's Lymphoma Cells.

The therapeutic potential of chemically synthesized AuNPs has been demonstrated in various types of cancer. However, gold nanoparticles (AuNPs) synthesized using typical chemical methods have concerns regarding their environmental safety and adverse impact on human well-being. To overcome this issue, we used an environmentally friendly approach in which gold nanoparticles were synthesized using Moringa oleifera leaf extract (MLE). The present research was mainly focused on the biosynthesis and characterization of gold nanoparticles (AuNPs) using Moringa oleifera leaf extract (MLE-AuNPs) and explore its anticancer potential against Dalton's Lymphoma (DL) cells. Characterization of the MLE-AuNPs was conducted using UV-Vis Spectroscopy to confirm the reduction process, FTIR analysis to ascertain the presence of functional groups, and XRD analysis to confirm the crystallinity. SEM and TEM images were used to examine size and morphology. After characterization, MLE-AuNPs were evaluated for their cytotoxic effects on Dalton's lymphoma cells, and the results showed an IC50 value of 75 ± 2.31 µg/mL; however, there was no discernible cytotoxicity towards normal murine thymocytes. Furthermore, flow cytometric analysis revealed G2/M phase cell cycle arrest mediated by the downregulation of cyclin B1 and Cdc2 and upregulation of p21. Additionally, apoptosis induction was evidenced by Annexin V Staining, accompanied by modulation of apoptosis-related genes including decreased Bcl-2 expression and increased expression of Bax, Cyt-c, and Caspase-3 at both the mRNA and protein levels. Collectively, our findings underscore the promising anti-cancer properties of MLE-AuNPs, advocating their potential as a novel therapeutic avenue for Dalton's lymphoma.

Effects of dragon fruits and moringa oleifera extracts, mineral mix variations on soft milk candy

Calcium is the most micronutrient found in dragon fruit reaching 8.5 mg/100 grams. Other sources of calcium in humans’ diet as milk, and moringa oleifera leaf can be used alternated to accept a daily intake of 1000 mg per day to support the formation of bones and teeth. The purpose of the study was to determine of calcium content from dragon fruits and moringa oleifera extracts, mineral mix variation on milk soft candy. This study is early to make milk soft candy with a completely randomized design (CRD) as P1 (12:1:50:8); P2 (14:2:50:9) and P3 (16:3:50:10) of dragon fruit extract: moringa oleifera leaf extract: mineral mix, with two replication. All treatments are tested of calcium content using An atomic Absorption Spectrophotometer (AAS), and analyzed using a factorial design analysis of variance (ANOVA) test. The result showed the difference in calcium levels between treatments was significant (p: 0,000). The highest calcium content obtained on P1 treatment (12:1:50,8 dragon fruit extract : moringa Oleifera leaf extract : mineral mix) was 1,593 mg/100g100g.

Characterization and antibacterial activity of bacterial cellulose impregnated with Moringa oleifera leaf extract and silver nanoparticles

Characterization and antibacterial activity of bacterial cellulose impregnated with Moringa oleifera leaf extract and silver nanoparticles

Moringa oleifera leaf extracts improve exercise performance in young male adults: A pilot study

BackgroundMoringa oleifera leaves are rich in bioactive substances. PurposeThe purpose of this study was to evaluate the effects of Moringa oleifera leaf aqueous extract supplements on energy metabolism and antioxidant function in young male adults. MethodsForty-four young male adults (26.3 ± 3.5 years) were randomly assigned to two groups: a supplement group (n = 23) receiving aqueous extract of Moringa oleifera leaves and a placebo group (n = 21). The supplementation period lasted for 30 days. Baseline measurements were taken at the beginning of the study, and further measurements were taken at the end of the supplementation period. Changes in upper- and lower-body strength, treadmill endurance, and certain blood biochemical parameters were evaluated. ResultsAfter 30 days of supplementation, participants in the supplement group exhibited enhanced performance in push-ups and treadmill exhaustion tests compared to the placebo group. Levels of glucose, urea, malondialdehyde, and glutathione peroxidase activity in serum were also improved in the supplement group. ConclusionThe findings suggest that Moringa oleifera leaf aqueous extracts have the potential to improve post-exercise energy metabolism and antioxidant function in young male adults.

Neuroprotective and antioxidant potential of Moringa oleifera leaf extract in a murine model of Parkinson's disease

Neuroprotective and antioxidant potential of Moringa oleifera leaf extract in a murine model of Parkinson's disease

Evaluation of Moringa Oleifera Leaf Extract for its In vitro Antibacterial Properties, Mechanism of Action, and In vivo Corneal Ulcer Healing Effects in Rabbits' Eyes.

M. oleifera is the most adapted tree species in different medicinal eco-systems and has resilience against climate changes. This multiple-use tree provides healthy foods, snacks, honey, and fuel. Besides this, it has immense promising applicationsby offering antimicrobial and antibacterial activities for targeted uses. This validates the court of Hippocrates that let food be the medicine and medicine be the food for which moringa qualifies. In view of this, the antioxidant and in vitro antibacterial potency of the hydro-ethanolic extract of M. oleifera was evaluated on clinically isolated multidrug-resistant strains of Staphylococcus aureus. Furthermore, in vivo, the healing response of M. oleifera extract was analysed on corneal ulcers induced in rabbit eyes infected with methicillin-resistant Staphylococcus aureus. TheM. oleifera extract exhibited exponential antioxidant activity. In-vitro antibacterial activity was evaluated by agar well diffusion assay showing zone of inhibition ranging from 11.05±0.36 to 20±0.40 mm at concentrations of 20, 40, 80, and 160 mg/ml, whereas, in our finding, no zone of inhibition was observed below 20 mg/ml concentration, which indicated that there is threshold limit below which the antibacterial activity of M. oleifera extract is not observed. Furthermore, continuous application of 3% and 5% M. oleifera extract (eye drop) four times a day for 14 consecutive days showed a significant healing response of the eyes of rabbits with corneal ulcers. These results suggest that M. oleifera extract could be a viable alternative to existing antibacterial therapies for corneal ulcers. Additionally, there is a possibility of commercial formulation of M. oleifera extract in the form of deliverable pharmaceutical products; therefore, it should be explored further.

Synergism of Anti-Malarial Effect of Carica papaya L. and Moringa oleifera Leaf Extracts in Mice

Malaria parasites, Plasmodium spp, can develop resistance to anti-malarial drugs. Hence, potential alternative therapeutic agents should be explored. This research aimed to investigate the anti-malarial effect of a combination of Carica papaya and Moringa oleifera leaf extract. Peter’s test performed on Balb/c mice to investigate the malarial parasite growth inhibition. Mice were infected with Plasmodium berghei by intraperitoneal injection to provoke rodent malaria. After infection, we grouped every 5-6 mice into different treatment groups, including a negative control group, a positive control group treated with artesunate, a moringa group, a papaya group, and a combination therapy group. Treatments were initiated 3 hours after infection (day 0) and continued every day until day 3. On day 4, we examined a thin smear of the tail vein blood for parasitemia to calculate the suppression rate. The result shows that the parasite suppression rate of C. papaya leaf extract (CPLE) is 15.02 % (percent parasitemia 4.56±1.96), the suppression rate of M. oleifera leaf extract (MOLE) is 17.32% (percent parasitemia 4.44±1.78) and that of the combination extract (CE) is 28.73% (percent parasitemia 3.82±1.48). In conclusion, there is a synergism of the anti-malarial effect of the combined leaf extract of C. papaya and M. oleifera.

Eco-friendly zinc oxide nanoparticles from Moringa oleifera leaf extract for photocatalytic and antibacterial applications

Eco-friendly zinc oxide nanoparticles from Moringa oleifera leaf extract for photocatalytic and antibacterial applications

Moringa oleifera Leaf Extract and (Amorphophallus muelleri Blume.) Glucomannan Effects on Obese White Rat Feed Intake and Body Weight

Obesity significantly raises the rates of illness and death in humans due to its association with several health conditions such as cardiovascular disease, hypertension, hypercholesterolemia, type 2 diabetes mellitus, osteoporosis, depression, breast cancer, and colon cancer. This study aims to determine the effect of Moringa oleifera leaf extract and glucomannan (Amorphophallus muelleri Blume.) on feed intake and body weight of obese white rats. This study used an experimental method with a pretest-posttest-only control grub design using 24 white male rats of the Wistar strain aged 8 weeks which were divided into 2 control groups, namely positive(K1) and negative(K2) and six treatment groups with extract doses Moringa oleifera leaves and glucomannan (Amorphophallus muelleri Blume.) 100mg/kgBW:100mg/kgBW(K3), 80mg/kgBW:120mg/kgBW(K4), 120mg/kgBW: 80mg/kgBW(K5), 50mg/kgBW:50mg/kgBW(K6), 40mg/kgBW:60mg/kgBW(K7), and 60mg/kgBW:40mg/kgBW(K8). Data analysis on body weight and feed intake used the SPSS ANOVA test and paired T-test. The results of the paired T-test (p<0.05) showed that Moringa oleifera leaf extract and glucomannan (Amorphophallus muelleri Blume.) significantly reduced body weight and feed intake. In conclusion, administration of Moringa oleifera leaf extract and glucomannan (Amorphophallus muelleri Blume.) in group K4 (80 mg/kgBW Moringa oleifera leaf extract: 120 mg/kgBW glucomannan Amorphophallus muelleri Blume) can reduce feed intake (4.29 g) and body weight (36.67 g) effectively.

The Effect of Moringa Leaf Extract (Moringa oleifera) in Drinking Water on Broiler Chicken Carcass Production

The research was carried out in the chicken house of the Faculty of Animal Husbandry, Marine and Fisheries, Nusa Cendana University, Kupang from April 7th 2023 to May 26th 2023. The aim of this research was to examine the effect of administering Moringa oleifera leaf extract in drinking water on broiler chicken carcass production. This research used 96 DOC broiler chickens of the CP 707 strain. The experimental design used was a Completely Randomized Design (CRD) with 4 treatments and 6 replications, each replication consisting of 4 broiler chickens. The treatments given were P0= drinking water without moringa leaf extract (control), P1= 40 ml moringa leaf extract/l drinking water, P2= 80 ml moringa leaf extract/l drinking water, P3= 120 ml moringa leaf extract/l drinking water. The variables studied were final body weight, carcass percentage, non-carcass percentage and abdominal fat. The results of statistical analysis show that moringa leaf extract at levels of 0, 40, 80 and 120 ml/l of drinking water did not have a significant effect (P>0.05) on final body weight, percentage of carcass and non-carcass percentage of broiler chickens, but increased the percentage of abdominal fat significantly (P<0.05). Based on the research results, it can be concluded that the use of Moringa oleifera leaf in drinking water up to a level of 120ml/l drinking water can increase the percentage of abdominal fat but did not change the final body weight, carcass percentage and non-carcass percentage of broiler chickens.

Assessment of Anticancer Activity of Crude Ethanolic Extracts of Moringa Oleifera Pod and Leaves on 7,12 - Dimethylbenz Anthracene Induced Skin Cancer in Mice.

Background and Objective: Carcinoma of the skin is the commonest cancer in the world. This study aims to assess the anti-cancer effect of the ethanolic pod and leaf extracts of Moringa Oleifera on 7,12 - dimethylbenz anthracene (DMBA) induced skin carcinoma in mice. Methodology: Animals were divided into 6 groups of 5 each. 7,12 - dimethylbenz anthracene (DMBA) was used topically for four weeks to induce tumour. Group 1 received placebo, Group 2 - standard drug 5- Fluorouracil, Groups 3, 4 received pod extract and Groups 5,6 received leaf extract of Moringa Oleifera of concentration 500 and 1000mg/kg respectively for 3 weeks. Hematological and biochemical parameters such as Hemoglobin, RBC, WBC and platelet counts, SGOT and SGPT, blood urea nitrogen and serum creatinine were done before cancer induction and at the end of 7 weeks. Histopathological examination of the skin, liver and kidney were done at the end of 7 weeks. Results: There was reduction in tumor size in the Moringa Oleifera pod and leaf extract treated groups. Histopathology revealed infiltration of the cells with scarring of epidermis in the extract treated groups indicating the healing of tissues more pronounced at higher concentration. Control group showed atypical squamous cells whereas the standard drug treated group showed infiltration and scarring. Conclusion: This study exhibits a dose dependent anticancer effect of Moringa oleifera pod and leaf extracts in mice which was comparable with the standard drug 5-Fluorouracil.

Effect of Moringa oleifera Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae Biotype 2.

The present study aimed to investigate the effects of Moringa oleifera leaf (MLE) extract-supplemented diets on the growth, feed utilization, hematology, innate immune response, and disease resistance of Nile tilapia against Streptococcus agalactiae Biotype 2. Four hundred and fifty Nile tilapia (32.61 ± 0.2 g/fish) were randomly allocated into fifteen tanks (30 fish/tank). Different concentrations of MLE at 0%, 0.5%, 1%, 1.5%, and 2% were fed to the Nile tilapia for 30 days, and the growth, feed utilization, hematology, and innate immune response of the Nile tilapia were determined. After the feeding trial, the Nile tilapia were challenged with a S. agalactiae Biotype 2 infection, and the relative percentage of survival (RPS) was determined. Results revealed the presence of quercetin, kaempferol, and p-coumaric acid in the MLE extract, exhibiting stronger antimicrobial activity against S. agalactiae Biotype 2. The diets supplemented with the MLE-0.5 group showed a significantly higher growth, feed utilization, hematology, and innate immune response in the Nile tilapia compared to the control and other MLE groups. Additionally, the MLE-0.5 group exhibited a significantly higher RPS of the Nile tilapia against S. agalactiae Biotype 2. Therefore, MLE-0.5 can be employed as an alternative feed supplement in sustainable Nile tilapia farming to protect against S. agalactiae Biotype 2.

Efficacy of moringa (Moringa oleifera) formulations on suppression of root-knot nematodes (Meloidogyne incognita) and growth of tomato (Solanum lycopersicum)

Root-knot (Meloidogyne sp.) nematodes are among the most important causes of reduced tomato (Solanum lycopersicum) yield. Therefore, an investigation was conducted in 2022 at the Aquaculture Research Unit lab to evaluate the effectiveness of moringa (Moringa oleifera) leaf extract application in controlling root-knot nematode in tomatoes. During a survey of a tomato field, M. incognita was identified from the rhizosphere soil samples. Fresh leaf extracts of moringa, including positive control (fenamiphos 0.06), were investigated in vivo for efficacy against M. incognita in root and soil. Seedlings of tomato were inoculated with 3000 eggs and second-stage juveniles (J2s) of M. incognita in pot trials, with treatments comprising fresh leaf extracts of moringa in a series of concentrations (40 ppm-80 ppm). Fifty-six days after inoculation, the phytonematicide had reduced eggs and J2s in soil by 65-90%, gall number by 45-87%, and gall index by 35-82%. The best treatment was moringa leaf extract (80 ppm), which was significantly different (P less than 0.05) from moringa leaf extract at 60 ppm and 40 ppm (P less than 0.05). However, fenamiphos had no significant difference (P greater than 0.05). The results showed moringa leaf extract (80 ppm) was the best treatment for controlling root-knot nematode in tomatoes.

GC–MS analysis of Moringa oleifera leaf extract and effects of administration on histology of reproductive organs and liver of female rats exposed to chronic unpredictable stress

Exposure to several stressors and other lifestyle factors converge to affect the chemistry, structure, and function of body tissues over time. Chronic unpredictable stress (CUS) protocol has been widely used to study the impact of stress exposure in several animal models. Moringa oleifera leaf extract (MOLE) is known to have hepatoprotective and antioxidant properties. The study aimed to determine the effect of the administration of MOLE during conditions of chronic stress on the histology of reproductive organs, heart and liver. Twenty mature inbred healthy virgin female Wistar rats were randomly placed into four groups of five rats per group as follows: Group One (water and normal rat chow ad libitum), Group Two (CUS protocol only), Group Three (CUS protocol + 200 mg/kg MOLE), Group Four (CUS protocol + 300 mg/kg of MOLE). CUS protocol was applied unpredictably for two weeks. The chromatogram of the GC–MS analysis indicated the presence of 41 compounds. Photomicrograph examination of the uterus, ovaries, and liver showed severe alterations in the histology of the tissues. Consumption of MOLE during chronic stress has shown deleterious effects and is not advised for consumption during stress conditions.

Moringa oleifera leaf extract promotes the healing of critical sized bone defects in the mandibles of rabbits

ObjectiveThe search for an osteopromotive material that enhances the efficacy of alloplasts in reconstructive surgeries has been going on for years. This study aimed to histologically and histomorphometrically evaluate the efficiency of Moringa oleifera leaf extract as an osteopromotive biomaterial.DesignThe study is a prospective randomized controlled animal study. 24 adult male New Zealand rabbits were equally allocated into test and control groups. Critical-sized bone defects were created in the edentulous areas of the mandibles of rabbits. The defects of the control group were filled with Beta-tricalcium Phosphate, while the defects of the test group were filled with Beta-tricalcium Phosphate combined with Moringa oleifera leaf extract. The results were evaluated histologically and histomorphometrically.ResultsHistological and histomorphometric analysis showed a significant increase in the surface area of bone and the number of osteoblasts in test groups compared to those in the control groups.ConclusionMoringa oleifera leaf extract has a positive effect on bone regeneration in critical-sized bone defects.

Green route to prepare zinc oxide nanoparticles using Moringa oleifera leaf extracts and their structural, optical and impedance spectral properties

This article investigates biosynthesis of zinc oxide (ZnO) nanoparticles (NPs) from Moringa oleifera leaves extract using an eco-friendly preparation method. The crystalline structure, optical properties, morphology and impedance characteristics of ZnO NPs were analyzed using impedance spectroscopy, powder X-ray diffraction (XRD), scanning electron microscopy, Fourier transform infrared spectroscopy (FTIR) and ultraviolet spectroscopy (UV-vis). The powder XRD pattern confirmed the crystallinity of the prepared samples as well as enabled determining their crystallite size and pure phase portion. The FTIR study confirmed the presence of functional groups responsible for reduction metal ions into ZnO NPs. UV-vis absorption spectra contained the absorption peak corresponding to ZnO NPs. Impedance spectroscopy of the prepared ZnO NPs revealed the grain boundaries in them and confirmed their semiconducting nature.

Efficiency of green synthesised carbon nanotubes from Moringa oleifera leaf extract as potential toxic metals adsorbent in polluted water

Abstract Among the many drawbacks of the current wastewater treatment systems are their high energy consumption and creation of harmful sludge. Carbon nanotubes (CNTs) are advantageous for the treatment of water due to their strong adsorption capacity and selectivity towards various pollutants. Thus, creating methods for treating water by producing CNTs from Moringa oleifera leaf extract can greatly help with the clean water problem. The synthesised material’s Ultra Visible (260–385 nm), FTIR (764–3295 cm−1) and Scanning Electron Microscope (SEM) characterizations have demonstrated their CNTs characteristics. The repeatability testing yielded the %RSD values for Ca = 0.4, Cu = 0.24, Mg = 0.9, Pb = 1.06 and Zn = 0.36 which suggest that the AES approach demonstrated a high degree of precision. The findings show that the quantity of recovered metals increases with retention period. Mg > Cu > Zn > Ca > Pb was the order of the metal adsorption capacity throughout retention durations of 3, 6, 24, and 48 h. As the initial concentrations of the components under research were doubled and tripled, so were the removal capacities of CNTs increased which may be as a result of an increase in metal ions in the solution. The outcomes show how well the synthetic CNTs can remove heavy metals from wastewater.

Microstructures, Optical, magnetic Properties, and photocatalytic activity of magnetically separable and reusable ZnO-Doped Fe3O4/rGO nanocomposite synthesized via green route

We report magnetically-separable, reusable, green-synthesized Fe3O4/rGO/ZnO, as heterogeneous catalyst for photo-Fenton degradation of organic pollutants in aqueous solution under certain treatments. Fe3O4 nanoparticles was green-synthesized using Moringa oleifera leaf extract, while rGO was synthesized utilizing Amaranthus viridis leaf extract. Fe3O4/rGO was composited under sonication treatment. Afterwards, Fe3O4/rGO was doped with ZnO with various concentration of ZnO. X-ray diffraction and selected area electron diffraction showed that Fe3O4 and ZnO had spinel cubic and hexagonal structure, respectively; another phase appeared as Fe2O3 spinel cubic structure. Crystallite size was decreased as the ZnO concentration increased. Morphology image showed almost spherical, non-uniform, and slightly dispersed particle under agglomerated condition, attaching to rGO sheets. The particle size of Fe3O4, Fe3O4/rGO, and Fe3O4/rGO/ZnO is 14.3; 14.1; and 10.4 nm, respectively. Fourier-transform infrared spectra showed metallic functional groups, such as Fe-O and Zn–O at 562–589 and 462–478 cm−1 also suggests nanocomposite formation. However, blue-shift absorption and band gap widening were observed with ZnO addition. Raman spectroscopy revealed the formation as-synthesized GO and rGO. Vibrating sample magnetometer showed that green-synthesized Fe3O4/rGO/ZnO exhibited superparamagnetic properties. Removal efficiency of photodegradation methylene blue was optimal for green-synthesized Fe3O4/rGO/ZnO under sonication treatment, reached 100 % degradation within 180 min for uptake every 30 min. Photodegradation was also analyzed using Langmuir-Hinshelwood kinetic model, resulting rate constant of 24.7 × 10−3 min−1 and half-life time of 28.1 min at optimum treatment. Reusability of photocatalytic activity after 3 cycles showed only a tiny drop in catalytic efficiency. Meanwhile, it possesses high stability in catalytic activity and structure. The green-synthesized Fe3O4/rGO/ZnO potential as an environmentally friendly reusable photocatalyst for wastewater degradation.