Moringa Oleifera Flower Extract Research Articles

Effect of Moringa Flower Extract (Moringa Oleifera) on Collagenization and Histopathology of Skin Tissue in the Healing of Dermapen Scars in Obese Male Wistar White Rats

Injury can be accidental, intentional, or disease-related. Moringa leaves may help heal wounds. The research aims to test and assess the effects of Moringa oleifera flower extract on dermapen wound healing collagenization in male Wistar strain white rats, an obese model, and their histological findings—quantitative True Experimental Study with Post-Test Only Control Group Design. Twenty-four male Wistar white rats (Rattus norvegicus) were sampled. Accumulation of Test Animals, Moringa Flower Extract Preparation, Phytochemical Screening / Secondary Metabolite Test, Treatment Process (200, 400, and mg/kgBW of Moringa flower extract), Wound Observation, Histopathological Skin Preparations, and Histopathological Examination of Preparations were the research procedures. Window data was analyzed using SPSS 25.0 and One Way ANOVA with a 95% confidence level (p < 0.05). The Post Hoc Test with LSD was used for further analysis. The research results found Moringa flower extract at 200mg/Kg BW, 400mg/KgBW, and 600mg/KgBW could expedite wound healing and improve collagen density in obese mice's dermapen wounds. The 600mg dose was most beneficial. Research conclusions: Moringa flower extract aids dermapen wound healing, collagenization, and obesity weight loss, according to a study.

Obesity Model in White Male Wistar Rats: The Impact of Moringa (Moringa Oleifera) Flower Extract on Pancreatic Function as Deduced from Serum Amylase and Lipase Levels and the Histopathological Image of the Pancreas

Diabetes and obesity go hand in hand. Antidiabetic drugs are effective in treating diabetes. However, currently available antidiabetic drugs are too expensive, do not work, and cause major side effects. A significant therapeutic option for Diabetes Mellitus and obesity is using bioactive chemicals produced by plants. This compound is efficacious, easily accessible, safe, and affordable. The main aim of this study was to determine whether pancreatic extract from Moringa oleifera flowers affected blood amylase and lipase levels, as well as the histological appearance, of obese male Wistar white rats (Rattus norvegicus). This is a True experimental study, with the research design used as a test control Group Design. The research data was then analyzed with the help of SPSS using ANOVA and Post Hoc Test with LSD technique. This researcher used 24 male rats. Treatment with Moringa oleifera flower extract improves pancreatic function in obese Wistar white rats by reducing serum lipase and amylase levels. The results of research on serum lipase and amylase levels showed that a dose of 600mg/Kg BW improved pancreatic function in white rats of the Wistar strain that were obese.

Anti-anemic potential of moringa oleifera flower extract against phenylhydrazineinduced anemia in rats

Anti-anemic potential of moringa oleifera flower extract against phenylhydrazineinduced anemia in rats

Structural, thermal, techno‐functional and chemical characterization using Fourier Transform Infrared Spectroscopy, Gas‐Chromatography‐Mass Spectrophotometry, Thermogravimetric Analyser, Field Emission Scanning Electron Microscopy and Energy‐Dispersive X‐Ray Spectrometer of Moringa Oleifera flower powder

SummaryThe aim of this research is to characterise the physicochemical and techno‐functional properties of the Moringa oleifera flower powder, and various techniques were used in this regard, such as FTIR, GC–MS, TGA, FESEM, and EDS. The bulk and tapped density of the Moringa oleifera flower powder were found to be 0.44 ± 0.03 (g/cm3) and 0.51 ± 0.01 (g/cm3), respectively. The water absorption index and water solubility index were 10.60 ± 0.10 (g/g) and 8.70 ± 0.20 (%), respectively. The foaming capacity was found to be 15.20 ± 0.20 (%), and the foaming stability was 56.34 ± 0.10 (%). The total phenolic content of the Moringa oleifera flower extract was 15.97 ± 0.223 mg GAE/g, whereas the flavonoid content was 12.38 ± 0.321 mg QE/g. The antioxidant activity of the flower extract was measured by DPPH assay and was found to be 59.65%. The flower extract showed 11 mm and 8 mm of the zone of inhibition against Escherichia coli and Staphylococcus aureus, respectively, whereas, with antibiotic streptomycin, the zones of inhibition were found to be 20 and 18 mm, respectively. Based on the research findings, Moringa oleifera flower powder and extract can be used as food fortification, antioxidant, and antibacterial agents.

Green Synthesis and Characterization of Nickel Nanoparticles using Aqueous Extract of Moringa oleifera Flower

An attempt has been made in this work to green synthesize nickel nanoparticles using the aqueous flower extract of Moringa oleifera, a common plant in which all parts are edible and rich in iron content, through the biosynthesis method. The synthesized Moringa oleifera flower extract nanorods were characterized by UV–Vis. spectroscopy, Fourier Transform Infrared spectroscopy, X-Ray Diffraction analysis and Transmission Electron Microscopy. In addition, the antimicrobial activity of the nanorods was evaluated. The nanorods were found to be crystalline in nature with rod-like structures having a mean particle size of 35 nm.

Structural, Optical, Magnetic and Dielectric Properties of CoFe12O19 Synthesized in the Presence of Moringa oleifera Flower Extract

In this study, a unique approach was employed to synthesize M-type cobalt hexaferrite (CoFe12O19) using Moringa oleifera flower extract as a green capping agent. For 3 h, the calcination procedure was conducted at 1100 ºC. It was found that the structural, optical, microstructural, magnetic and dielectric properties of the generated cobalt hexaferrite were considerably affected by the presence of phytochemicals in floral extract. To evaluate the structural, surface morphological, optical, magnetic and dielectric properties of prepared ferrite, a variety of characterization techniques including FT-IR, XRD, UV-Vis, SEM, VSM and dielectric techniques were applied. The XRD analysis showed the formation of a hexagonal phase along with hematite and cobalt ferrite, while the surface morphology showed hexagonal shaped platelet structure. With a saturation magnetization (Ms) of 14.94 emu/g and a coercivity (Hc) of 1790.9 Oe, the CoFe12O19 powder demonstrated a multidomain nature, which is a magnetically hard material. Furthermore, the energy bandgap of the cobalt ferrite was observed to be 2.01 eV. Low frequency dielectric measurement was carried out at room temperature and ε′max ~ 46 found at 30 Hz with tan δ equals to 0.103. The dielectric behaviour with frequency follows the Koop’s model and Maxwell-Wagner theory. One semicircle arc was observed in Cole-Cole plot.

Amelioration potential of Moringa oleifera extracts against sodium arsenate induced embryotoxicity and genotoxicity in mouse (Mus musculus).

Previous studies have suggested that arsenic crosses the placenta and affects the fetus development. The study under consideration aims to show comparative ameliorative effect of Moringa oleifera leaf and flower extracts against sodium arsenate induced fetus toxicity of mice. Pregnant mice (N=44) were kept in lab and divided into eleven group from (A to K) and were orally administered the doses 6 mg/kg, 12 mg/kg for sodium arsenate, 150 mg/kg and 300 mg/kg for Moringa oleifera leaf extracts (MOLE) and 150 mg/kg and 300 mg/kg for Moringa oleifera flower extracts (MOFE) comparing with control. The investigation revealed evident reduction in the fetuses weight, hind limb, fore limb, tail and snout length, crown rump and head circumferences well as malformations in tail, feet, arms, legs, skin and eyes in the negative control group (only administered with sodium arsenate). Co-administration of sodium arsenate with MOLE and MOFE ameliorate the reversed effect of sodium arsenate on the shape, length, body weight and DNA damage of fetus significantly at 95% confidence interval. However, Moringa oleifera leaf extract showed more significant results in comparison to Moringa oleifera flower extract. Hence concluded that Moringa oleifera leaf extract ameliorated the embryo toxic effects of sodium arsenate and can be used against environmental teratogens.

Microwave assisted green synthesis of Hydroxyapatite nanorods using Moringa oleifera flower extract and its antimicrobial applications

Hydroxyapatite is an important biomaterial and main mineral component found in bones for potential clinical applications. Moringa oleifera, a common plant in which all parts are edible and rich in iron content. This study reported the chemically synthesized Hydroxyapatite and green synthesis of Hydroxyapatite nanorods using the aqueous flower extract of Moringa oleifera by microwave assisted method. The synthesized Moringa oleifera flower extract Hydroxyapatite nanorods were characterized by UV–Vis spectroscopy (UV–vis), Fourier Transform Infra Red spectroscopy (FTIR), X-Ray Diffraction analysis (XRD), Transmission Electron Microscopy (TEM), Photo Luminescence spectroscopy (PL), Thermo Gravimetric analysis (TGA) and Energy Dispersive X-ray analysis (EDX). In addition, the antimicrobial activity of these nanorods was assessed. Moringa oleifera flower extract Hydroxyapatite nanorods were crystalline in nature, rod like structure with a mean particle size of 41 nm. The antibacterial activity of Moringa oleifera flower extract capped Hydroxyapatite nanorods was greater against Gram positive bacteria than Gram negative bacteria. Furthermore, Moringa oleifera extract capped Hydroxyapatite nanorods showed a very good antifungal activity against three common pathogenic fungi including; Candida albicans, Aspergillus fumigatus and Aspergillus niger.

Anticancer potential of Moringa oleifera flower extract in human prostate cancer PC-3 cells via induction of apoptosis and downregulation of AKT pathway

Background: Moringa oleifera (MO), or the horseradish tree, is a pantropical species that is known by such regional names as benzolive, drumstick tree, kelor, marango, mlonge, mulangay, nebeday, saijhan, and sajna. Over the past two decades, many reports have appeared in mainstream scientific journals describing its anticancer properties of MO. While much of this recent enthusiasm indeed appears to be justified, it is critical to evaluate therapeutic activity of MO on prostate cancer to separate rigorous scientific evidence from anecdote. MO contained active polyphenols such as ellagic acid, gallate, methyl gallate, catechol, kaempferol quercetin, and their derivatives. Objective: It is the purpose of this series of brief reviews to critically assess the efficacy of MO flower extract as an antiprostate cancer agent. Materials and Methods: The cell viability of the extract was determined using MTT in different time point and cell cycle progression studies was analyzed by flow cytometry. Various markers of apoptosis were evaluated by immunoblotting. Results: We observed that prostate cancer cells treated with MO flower extract caused 50% inhibition at a dose of 22.61 μg/mL and 6.25 μg/mL in PC-3 cells at 24 and 48 h, respectively. MO flower extract induced the accumulation of G1 phase cell cycle arrest and apoptosis by annexin V staining. Further, immunoblot detection of PARP cleavage leads to increase the protein expression of caspase-3 activity and Bax indicates induction of apoptosis. Conclusion: Together, the results suggest for the first time that administration of MO flower extract inhibits prostate cancer progression in PC-3 cells by interfering AKT pathway. Abbreviation used: MO: Moringa oleifera; PARP: Poly (ADP-ribose) polymerase; BAD: Bcl-2-associated death promoter; FACS: Fluorescence-activated cell sorting.

A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells

In this study, Moringa oleifera flower extract and a trypsin inhibitor (MoFTI) isolated from it were evaluated for anti-protozoal activity against Trypanosoma cruzi and cytotoxicity to mammalian cells. The presence of flavonoids was remarkable in the HPLC fingerprints of the extract at 254 and 360 nm. Amino acid sequences of peptides derived from in-gel digestion of MoFTI were determined. Both the extract and MoFTI caused lysis of T. cruzi trypomastigotes with LC50/24 h of 54.18 ± 6.62 and 41.20 ± 4.28 μg/mL, respectively. High selectivity indices (7.9 to >12) for T. cruzi cells over murine peritoneal macrophages and Vero cells were found for the extract and MoFTI. The results show that MoFTI is a trypanocidal principle of the flower extract.

Biosynthesis of palladium nanoparticles by using Moringa oleifera flower extract and their catalytic and biological properties

The biosynthesis of nanostructured biopalladium nanoparticles (PdNPs) from an aqueous solution of crystalline palladium acetate is reported. For the synthesised PdNPs in solution, an agroforest biomass waste petal of Moringa oleifera derived bis-phthalate was used as natural reducing and biocapping agents. Continuous absorption in the UV region and subsequent brown colour change confirmed the formation of PdNPs. A strong surface plasmon peak for PdNPs occurred at 460nm. PdNPs were characterized by SEM with EDX, FTIR, TEM and DLS. The chemical composition of the aqueous extract was determined by GC–MS coupled with FTIR and 1NMR. The catalytic degradation effect by PdNPs on industrial organic toxic effluents p-nitrophenol (PNP) and methylene blue dye was monitored by UV Spectroscopy. On the other hand PdNPs catalysed the base mediated suzuki coupling reaction for biphenyl synthesis, in water. Moreover, PdNPs were found to be reusable catalysts. Toxicity studies of PdNPs showed that the death of brine shrimp to be <50%. Therefore, PdNPs displayed potential for further anticancer studies via tumour cell lines. The in vitro cytotoxicity evaluation of the extract capped nanoparticles was carried out using human lung carcinoma cells (A549) and peripheral lymphocytes normal cells by MTT cell viability assay. Also, PdNPs showed antibacterial activity against Enterococcus faecalis among the different tested strains, including Bacillus cereus, Staphylococcus aureus, Esherichia coli and Candida albicans, Candida utilis.

Probing Regenerative Potential of Moringa oleifera Aqueous Extracts Using In vitro Cellular Assays.

Background:Molecules stimulating regeneration and proliferation of cells are of significance in combating ailments caused due to tissue injury, inflammation, and degenerative disorders. Moringa oleifera is one of the most valued food plants having the profile of important nutrients and impressive range of medicinal uses.Objective:To evaluate the potential of M. oleifera aqueous leaf and flower extracts to promote the proliferation of cells and explore their effect on cancer cell lines for assessment of safety.Materials and Methods:Aqueous leaf and flower extracts of M. oleifera were investigated for effect on rat-derived primary fibroblast, mesenchymal stem cells (MSCs), and cancer cell lines using cell proliferation assay. They were also tested and compared for wound healing, angiogenesis, and hepatoprotective effect using in vitro assays.Results:Statistically significant increase in the proliferation of primary rat fibroblast, MSCs, and angiogenesis was observed after treatment with aqueous flower extract. The aqueous leaf extract determined a comparatively moderate increment in the proliferation of MSCs and angiogenesis. It however showed prominent cytotoxicity to cancer cell lines and a significant hepatoprotective effect.Conclusion:A very clear difference in response of the two extracts to different types of cells was detected in this study. The aqueous flower extract exhibited a higher potential to stimulate cell proliferation while not exerting the same effect on cancer cell lines. The leaf extract on the other hand, had a prominent antitumor and hepatoptotective effects.SUMMARY Moringa oleifera flower extract showed significant ability to promote proliferation of rat fibroblast and mesenchymal stem cells. The extract also had prominent angiogenic and hepatoprotective effects.The extract did not influence proliferation of cancer cell lines indicating its safety for human consumption and use in pharmaceuticals.The Moringa oleifera leaf extract showed relatively less potential to stimulate cells but had prominent cytotoxic effect on cancer cell lines. Abbreviations Used: ALT: Alanine transaminase, AST: Asparatate amino transferase, ATCC: American type culture collection, BMMSC: Bone marrow mesenchymal stem cells (used in this paper), CAM: Chick chorioallantoic membrane, CCl4: Carbon tetra chloride, DMEM: Dulbecco's modified Eagle medium, DMSO: Dimethyl sulfoxide, EDTA: Ethylene diamine tetraacetic acid, HBL 100: Human breast epithelial cell line, Mcf-7: Human breast adenocarcinoma cell line, aMEM: Minimum Essential Medium Eagle alpha modification, MOF: Moringa oleifera aqueous flower extract (used in this paper), MOL: Moringa oleifera aqueos leaf extract (Used in this paper), OD: Optical density, PBS: Phosphate buffered saline

The ionic liquid assisted green synthesis of hydroxyapatite nanoplates by Moringa oleifera flower extract: A biomimetic approach

This report deals an attempt for a successful green synthesis of hydroxyapatite nanoplates assisted from Moringa oleifera flower extract and 1-butyl-3-methylimidazolium tetrafluoroborate ionic liquid. The prepared nanoplates were calcinated at two different temperatures and the crystallinity, particles size and morphology were studied by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), high resolution transmission electron microscopy (HRTEM) and atomic force microscopy (AFM) techniques. These results proved the crystal growth nucleation of HAp nanoplates at different calcination temperatures. FTIR results were established the interaction of ion precursors with polyphenolic hydroxyl groups from flower extract. The important role of ionic liquid assessment also confirmed the formation of amorphous into crystalline plate like structures.

Natural Anti-Inflammatory Products/Compounds: Hopes and Reality.

Inflammation is a complex biological response to injury as a result of different stimuli such as pathogens, damaged cells, or irritants. Nowadays, the commercially approved anti-inflammatory drugs are represented by nonsteroidal anti-inflammatory drugs (NSAID), glucocorticoids (SAID), and in some cases immunosuppressant and/or biological drugs. These agents are effective for the relief of the main inflammatory symptoms. However, they induce severe side effects, whereas most of them are inadequate for chronic use. Starting from these premises, the demand of new effective and safely anti-inflammatory drugs has furthered research into new therapeutic approaches. The recent and emerging scientific community slant is oriented to the herbal medicines that could represent a treasure for the discovery of new active compounds and for the development of new drugs and potentially useful therapeutic agents. The articles contained in this special issue include both reviews and basic scientific studies focused on characterizing the molecular mechanisms of the inflammatory process in humans and also in animal models. In particular, on this special issue the attention on the role of proinflammatory mediators and pathways such as IL-1, IL-6, mPGES-1, and NF-B had been pointed out (“Moringa oleifera Flower Extract Suppresses the Activation of Inflammatory Mediators in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages via NF-κB Pathway” by W. S. Tan et al. and “Downregulation of mPGES-1 Expression via EGR1 Plays an Important Role in Inhibition of Caffeine on PGE2 Synthesis of HBx(+) Hepatocytes” by Y. Ma et al. and “Anti-Inflammatory Effect of 1,3,5,7-Tetrahydroxy-8-isoprenylxanthone Isolated from Twigs of Garcinia esculenta on Stimulated Macrophage” by D.-D. Zhang et al.). Moreover, this special issue has highlighted the importance of some mechanism and strategy involved in inflammatory bowel disease and experimental model of osteoarthritis (“Botanical Drugs as an Emerging Strategy in Inflammatory Bowel Disease: A Review” by F. Algieri et al. and “Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of Osteoarthritis” by Z. Yan et al.). Finally, interesting contributions regarding the anti-inflammatory activity of marine diterpenoids and leishmaniasis inflammatory response have been successfully submitted to this special issue (“Marine Diterpenoids as Potential Anti-Inflammatory Agents” by Y. Gonzalez et al. and “Natural Products: Insights into Leishmaniasis Inflammatory Response” by I. A. Rodrigues et al.). We hope that this special issue will stimulate the interest of the scientific community involved in studying the effects of natural products/compounds on different fields of interests such as inflammation and pain. The papers published here will surely contribute to proposing new additional insights into the mechanism of several conditions as well as to suggesting new diagnostic alternatives and therapeutic targets in widespread pathologies. The discovery of the new is, as always, anchored to the recourse of the old.

Trypsin inhibitor from Moringa oleifera flowers interferes with survival and development of Aedes aegypti larvae and kills bacteria inhabitant of larvae midgut

Moringa oleifera flower extract, with trypsin inhibitor activity, is a larvicidal agent on Aedes aegypti. This work reports the isolation of trypsin inhibitor (M. oleifera flower trypsin inhibitor (MoFTI)) and its effect on A. aegypti egg hatching, viability of newly hatched larvae, survival of pupae, and growth of inhabitant bacteria from midgut of fourth-instar larvae (L4). MoFTI (K i, 2.4 μM), isolated by affinity chromatography on trypsin-agarose column, was an 18.2 kDa polypeptide on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Flower extract (at concentrations of 8.5-17.0 mg/mL) reduced egg hatchability while MoFTI (0.05-0.5 mg/mL) did not affect the hatching rate. Mortality of newly hatched larvae ranged from 3.5 to 19.1 % in the presence of the extract (4.0-17.0 mg/mL) and was also promoted by MoFTI (LC50, 0.3 mg/mL). After 72 h, larvae incubated with extract at 13.0 and 17.0 mg/mL were at stages L2 and L1, respectively, while in control they reached L3 instar. In the presence of MoFTI, at all concentrations tested, the larvae did not pass the first instar. Flower extract and MoFTI did not interfere on pupae survival. The extract and MoFTI inhibited the growth of L4 gut bacteria (minimum inhibitory concentrations of 3.47 and 0.031 mg/mL, respectively) but only the inhibitor showed bactericide effect (minimum bactericidal concentration of 1.0 mg/mL). The findings reported herein indicate that MoFTI constitutes a larvicidal principle from M. oleifera flowers against A. aegypti newly hatched larvae and is an antibacterial agent active against the microbiota from L4 gut.

Synthesis, characterization and SERS activity of biosynthesized silver nanoparticles

Silver nanoparticles were rapidly synthesized using Moringa oleifera flower extract as the reducing agent shows surface plasmon resonance peak at 439nm. The size and shape of the nanoparticles controlled by varying the concentration of M. oleifera flower extract in the reaction medium. The synthesized silver nanoparticles were well-dispersed spherical nanoparticles with the average size of 14nm. The retinoic acid present in M. oleifera flower extract used as reducing agent and proteins was responsible for capping of the bioreduced silver nanoparticles. The obtained nanoparticle shows size-dependent SERS activity. The SERS spectrum indicates that the pyridine adsorbed on the silver surface in a stand-on orientation via its nitrogen lone pair electrons.

EFFECT OF Moringa oleifera FLOWER EXTRACT ON LARVAL TRYPSIN AND ACETHYLCHOLINESTERASE ACTIVITIES IN Aedes aegypti

Aedes aegypti control is crucial to reducing dengue fever. Aedes aegypti larvae have developed resistance to organophosporous insecticides and the use of natural larvicides may help manage larval resistance by increasing elements in insecticide rotation programs. Here, we report on larvicidal activity of Moringa oleifera flower extract against A. aegypti L(1), L(2), L(3), and L(4) as well as the effect of flower extract on gut trypsin and whole-larval acetylcholinesterase from L(4.) In addition, the heated flower extract was investigated for larvicidal activity against L(4) and effect on larval gut trypsin. Moringa oleifera flower extract contains a proteinaceous trypsin inhibitor (M. oleifera flower trypsin inhibitor, MoFTI), triterpene (β-amyrin), sterol (β-sitosterol) as well as flavonoids (kaempferol and quercetin). Larvicidal activity was detected against L(2), L(3), and L(4) (LC(50) of 1.72%, 1.67%, and 0.92%, respectively). Flower extract inhibited L(4) gut trypsin (MoFTI K(i) = 0.6 nM) and did not affect acetylcholinesterase activity. In vivo assay showed that gut trypsin activity from L(4) treated with M. oleifera flower extract decreased over time (0-1,440 min) and was strongly inhibited (98.6%) after 310 min incubation; acetylcholinesterase activity was not affected. Thermal treatment resulted in a loss of trypsin inhibitor and larvicidal activities, supporting the hypothesis that flower extract contains a proteinaceous trypsin inhibitor that may be responsible for the deleterious effects on larval mortality.