AbstractKernels from Michigan‐grown tart cherry Prunus cerasus L. cv. Montmorency were frozen at −40°C, lyophilized, crushed and extracted sequentially with hexane and methanol. Amygdalin, mandelonitrile and benzaldehyde were present in the kernel extracts and were quantified by high performance liquid chromatography. Amygdalin was found to be the major component, while free mandelonitrile is detected for the first time in the tart cherry pit kernels.