Montane Forest Research Articles

Long-term soil warming changes the profile of primary metabolites in fine roots of Norway spruce in a temperate montane forest.

Climate warming poses major threats to temperate forests, but the response of tree root metabolism has largely remained unclear. We examined the impact of long-term soil warming (>14 years, +4°C) on the fine root metabolome across three seasons for 2years in an old spruce forest, using a liquid chromatography-mass spectrometry platform for primary metabolite analysis. A total of 44 primary metabolites were identified in roots (19 amino acids, 12 organic acidsand 13 sugars). Warming increased the concentration of total amino acids and of total sugars by 15% and 21%, respectively, but not organic acids. We found that soil warming and sampling date, along with their interaction, directly influenced the primary metabolite profiles. Specifically, in warming plots, concentrations of arginine, glycine, lysine, threonine, tryptophan, mannose, ribose, fructose, glucoseand oxaloacetic acid increased by 51.4%, 19.9%, 21.5%, 19.3%, 22.1%, 23.0%, 38.0%, 40.7%, 19.8%and 16.7%, respectively. Rather than being driven by single compounds, changes in metabolite profiles reflected a general up- or downregulation of most metabolic pathway network. This emphasises the importance of metabolomics approaches in investigating root metabolic pathways and understanding the effects of climate change on tree root metabolism.

Improving population size estimation at western capercaillie leks: lek counts versus genetic methods

The western capercaillie Tetrao urogallus, hereafter capercaillie, is the largest galliform bird present in the boreal and montane forests of the Western Palearctic. Precise and accurate methods for estimating the number of individuals and/or their densities are crucial for the proper management of its free‐ranging populations. However, obtaining reliable estimates of the abundance of populations of wild species and, particularly, of birds is not a simple task. In the case of lek‐mating birds such as capercaillie, surveys are traditionally based on lek counts, that is, counts of calling males present in their mating areas: the leks. This study was carried out on the Pyrenees at six capercaillie leks where two different lek counting approaches were performed: hide‐based and walk‐based. The results were compared with those obtained from an estimate of minimum population size (MPE) derived from genotyping all faeces samples found in the lek area, and with a population size estimate derived from a genetic mark‐recapture model () of each capercaillie lek. The results of were used to estimate the detection rate (D) of both lek count approaches. Our results show that traditional lek counts do not detect all male capercaillies since the detection rate was 0.34 (95% CI: 0.26–0.43) for hide‐ and 0.56 (95% CI: 0.43–0.68) for walk‐based lek counts. Our results suggest that the walk‐based lek counts were more efficient than the hide‐based ones, providing more accurate results compared to the estimate. The combination of non‐invasive sampling with genetic mark‐recapture model was found to be the most reliable method for obtaining the of leks given that traditional lek counts underestimate the number of capercaillie and, furthermore, can cause disturbance to the species at these sites.

Factors affecting the geochemistry of rare earth elements in soils in tropical rain and montane forests in Sri Lanka across an elevation gradient

Factors affecting the geochemistry of rare earth elements in soils in tropical rain and montane forests in Sri Lanka across an elevation gradient

Two new species of the treehopper genus Enchenopa Amyot & Serville, 1843 (Hemiptera, Membracidae) from northwest Ecuador.

Enchenopa Amyot & Serville, 1843 is a diverse treehopper genus widespread across the New World. We describe two new Enchenopa species from northwest Ecuador: Enchenopagennyae sp. nov. from urban forest remnants at the foothills of the Andes cordillera and Enchenopachocoandina sp. nov. from secondary montane forests. Enchenopagennyae sp. nov. is placed in the E.biplaga species group and is distinguished by the sexual dimorphism of the pronotal horn and lateral carina shape, the straight metopidium, 2-4 accessory carinae and the whitish dorsal spot and subapical band. Enchenopachocoandina sp. nov. belongs to the E.andina species group and is diagnosed by its reddish central carina and posterior pronotal process apex, presence of an obtuse projection rather than an anterior horn, three or four irregular accessory carinae, and apical amber forewing patch. Illustrations, notes on natural history, and keys to species of the E.biplaga and E.andina species groups are also provided.

Turbulent Energy and Carbon Fluxes in an Andean Montane Forest—Energy Balance and Heat Storage

High mountain rainforests are vital in the global energy and carbon cycle. Understanding the exchange of energy and carbon plays an important role in reflecting responses to climate change. In this study, an eddy covariance (EC) measurement system installed in the high Andean Mountains of southern Ecuador was used. As EC measurements are affected by heterogeneous topography and the vegetation height, the main objective was to estimate the effect of the sloped terrain and the forest on the turbulent energy and carbon fluxes considering the energy balance closure (EBC) and the heat storage. The results showed that the performance of the EBC was generally good and estimated it to be 79.5%. This could be improved when the heat storage effect was considered. Based on the variability of the residuals in the diel, modifications in the imbalances were highlighted. Particularly, during daytime, the residuals were largest (56.9 W/m2 on average), with a clear overestimation. At nighttime, mean imbalances were rather weak (6.5 W/m2) and mostly positive while strongest underestimations developed in the transition period to morning hours (down to −100 W/m2). With respect to the Monin–Obukhov stability parameter ((z − d)/L) and the friction velocity (u*), it was revealed that the largest overestimations evolved in weak unstable and very stable conditions associated with large u* values. In contrast, underestimation was related to very unstable conditions. The estimated carbon fluxes were independently modelled with a non-linear regression using a light-response relationship and reached a good performance value (R2 = 0.51). All fluxes were additionally examined in the annual course to estimate whether both the energy and carbon fluxes resembled the microclimatological conditions of the study site. This unique study demonstrated that EC measurements provide valuable insights into land-surface–atmosphere interactions and contribute to our understanding of energy and carbon exchanges. Moreover, the flux data provide an important basis to validate coupled atmosphere ecosystem models.

The Historiography of Forests use Types and Implication for Forest Conservation in the Montane Forests of Ngongbaa, Kilum, Kovifem and Kovkinkar

This study explores the timeline history of forest use types and beneficial relationship of forest, forest communities and conservation in the montane forests of Ngongbaa, Kilum, Kovifem and Kovkinkar from the mid-1970s to 2015. Data and/or information collected for this study comprise literary, questionnaire, interview, focused group discussions and observations. Data for the study was secured from person directly resident adjacent forest in 29 villages. The study shows that apart from being asource of food and medicine, forest first served as settlement and refuge sites for the Nso, Mbiame and Oku people. Today, forest serves as cemetery for the fons (kings), and host shrines which have become conservation hot spots and nurseries for engendered plants and animal species, where the Nso, Mbiame and Oku also performed sacrifices. These conservation hot spots help to sustain the forest in montane ecosystems where pressure on land and forest is high and on the rise. Due to population pressure and limited grazing the grassland compartments in the above forests have been transformed into grazing ground. The long period of intercourse between the Nso, Mbiame, Oku people and their forest enabled them to accumulate rich and useful knowledge of the forest and established customary (non- statutory or quasi-statutory) regulatory frameworks for effective management of forest resources to improve their livelihood and culture. Due to the depletion of forest and the adoption of stricter conservation policies, some forest activities particularly carving and hunting have decreased in intensity while some like Ngwa’a (royal hunt) has simply phased out due to the extinction of animal species which were hunted. The paper recommends that traditional systems of managing forest be reinforced to enhance the conservation of resources in sacred groves that are nurseries for threatened and extinct species in degraded ecosystems. The state should recognise and integrate non-statutory forestry policy frameworks established in the Nso, Mbiame and Oku into the national forestry policy as village-based institutions for the management of land and forest in forest communities. The government should also empower traditional authorities (Fon,Nwerong and Lanlords (ataangven) and support reforestation and forest restoration programmes formulated by them. This is because laws put in place by these institutions are respected in the most part by their subjects without being forced to do so, unlike in the Ngongbaa and Kilum forests managed by the state, where forestry laws are permanently violated by local people who consider forestry authorities as state agents who are depriving them of their right of tenure and usufruct. This would likely help to maintain biodiversity and knowledge attached to it, which is gradually disappearing due to forest depletion.

Three new species of parasitoid wasps of the genus Mnioes Townes, 1946 (Ichneumonidae: Banchinae) in a humid forest in Peru

Mnioes is a predominantly Neotropical genus of Banchinae (Ichneumonidae), currently including 26 species. Three new species of Mnioes are described, found in a humid montane forest in southern Peru, M. chunka sp. nov, M. chusaq sp. nov. and M. isqun sp. nov, and the male of M. huk Alvarado 2020 is also described. DNA barcoding for all the species treated here is included and provides information to match females with males, especially for M. chunka sp. nov., M. chusaq sp. nov., and M. soqta Alvarado 2020 where females are similar to each other and occur in sympatry. Additionally, an updated key to the Peruvian species is provided.

Transpiration and plant hydraulics of Abies veitchii under fluctuating environmental conditions in cool montane forest

AbstractIn subalpine fir wave forests, strips of dead and weakened trees occur perpendicular to the slope next to strips of healthy trees. To reveal the transpiration by weakened Abies veitchii trees exposed to increased atmospheric evaporative demand, we investigated the ecophysiological traits closely related to the growth and transpiration, comparing them with those of the healthy trees and saplings in the fir wave of Mt. Shimagare in central Japan. The transpiration rate (E) was investigated using sap flux sensors to measure heat pulse velocity and compared with the surrogate for the needle water demand, which was computed using a multilayered gas and energy transfer model (modeled E, Emod). Weakened trees exhibited smaller diameter growth and narrower sapwood than healthy trees, as well as lower heat pulse velocity compared with healthy saplings. However, needle‐level traits did not differ significantly between weakened and healthy trees. Needle water potential at midday was as negative as the needle turgor loss point, and the measured heat pulse velocity increased linearly with Emod but leveled off above a certain Emod value in weakened trees and healthy saplings, suggesting that trees restricted E to balance the needle water budget. Heat pulse velocity of weakened trees leveled off at Emod lower than that of healthy saplings, probably due to lower capacity for water supply to the needles. Restriction of E would occur less frequently but be necessary for both weakened and healthy A. veitchii on Mt. Shimagare to avoid hydraulic failure, sacrificing photosynthetic carbon assimilation.

A new endemic Begonia species (sect. Knesebeckia, Begoniaceae) from the south-western montane forest remnants of Ecuador

Begonia jocotocoi is a new species described from the Buenaventura Reserve in El Oro Province, south-west Ecuador. The new species morphologically resembles Begonia serotina, but differs by its minutely bullate leaf lamina (vs. smooth), the dense red long trichomes that cover the petioles and the lower surface of the leaf lamina, the latter of which distinctly become purple at maturity (vs. usually glabrous or occasionally with an indument of minute glandular white hairs and pale green below), presence of a pair of bracteoles directly beneath the pistillate flowers (vs. bracteoles absent), and the pedicels and also sometimes the abaxial surfaces of most floral whorls having a sparse cover of glandular hairs (vs. glabrous to puberulous). Photos of the new species and a distribution map are provided. Relationships with other species are discussed and the species’ preliminary conservation status is assigned as critically endangered (CR).

Phylogenetic structure and turnover between lowland and montane subtropical forests indicate differential community assembly processes, affected by successional stage and spatial gradients

Secondary forests represent an increasing proportion of global forest cover and offer a range of ecosystem services that are integral to environmental policy targets. Highly disturbed landscapes with temporal records of forest plant community composition offer key insights into the processes that shape plant communities as forests undergo secondary succession. We investigated patterns of plant community phylogenetic structure and phylogenetic beta diversity. Mean pairwise distance (MPD) and Mean Nearest Taxon Distance (MNTD) metrics were determined between co-occurring species within plant communities, in addition to phylogenetic beta diversity metrics relative to null models of random phylogenetic assembly. MPD and MNTD were compared between elevational and successional classes and modelled as products of intercommunity distance (metres) and forest community age (years). Phylogenetic nonmetric multidimensional scaling explored phylogenetic community structure between lowland and montane forest type in Hong Kong. We found that plant communities in secondary forests in Hong Kong exhibited patterns of increased basal phylogenetic clustering with increasing community elevation, while phylogenetic turnover was influenced by spatial and successional factors in lowland and montane forest. Our findings indicate differential community assembly in lowland compared to montane forest type in Hong Kong, as well as evidence for barriers to plant dispersal in secondary forest communities due to patterns of spatial phylogenetic clustering. Plant communities in montane forests were found to be phylogenetically distinct from lowland forest plant communities. Patterns of spatial phylogenetic clustering may suggest significant dispersal or post-dispersal processes causing the clustering of related species at fine spatial scales, while phylogenetic turnover with increasing successional age may indicate compositional changes during the process of passive forest regeneration. Collectively, these results emphasize the need to investigate active pathways for rewilding dispersal limited late successional forest tree species in Hong Kong.

Monitoring the rewilding of the Brazilian Atlantic Forest on tree and mammal diversity: From a biodiversity hotspot to a biodiversity hopespot

The implementation of rewilding initiatives is crucial for biodiversity recovery, particularly in biodiversity hotspots such as the Brazilian Atlantic Forest. Long-term monitoring of rewilding initiatives is critical for assessing progress and informing management choices. This study assesses the efficiency of different reforestation practices for biodiversity recovery, including passive restoration, active reforestation with different native seedlings, and plantations of exotic species. We applied an ecosystem approach to estimate the tree and mammal diversity, collected through forest plots and camera traps. We selected differently managed areas and included the oldest forest patches as reference points for the assessment of biodiversity; a naturally disturbed montane forest in the area was included as a control category for the disturbance analysis. Moreover, we investigated the influence of ecological variables, such as biodiversity, aboveground biomass, and altitude, on rewilding processes. Our results suggest that Eucalyptus plantations, a simplified ecosystem made up of monocultures of exotic species, support lower biodiversity. On the contrary, active reforestation with different native species resulted in an ecological status comparable to naturally regrown secondary forests. We show that active reforestation can reduce the time required for rewilding. We also found a positive correlation between tree and mammal species richness. These results underscore the importance and potential of responsible restoration efforts in recovering local biodiversity, contributing to the vision of a future where the Brazilian Atlantic Forest can be acknowledged not only as a biodiversity hotspot but also as a “hopespot.”

Energy‐Water Asynchrony Principally Determines Water Available for Runoff From Snowmelt in Continental Montane Forests

ABSTRACTChanges in the volume, rate, and timing of the snowmelt water pulse have profound implications for seasonal soil moisture, evapotranspiration (ET), groundwater recharge, and downstream water availability, especially in the context of climate change. Here, we present an empirical analysis of water available for runoff using five eddy covariance towers located in continental montane forests across a regional gradient of snow depth, precipitation seasonality, and aridity. We specifically investigated how energy‐water asynchrony (i.e., snowmelt timing relative to atmospheric demand), surface water input intensity (rain and snowmelt), and observed winter ET (winter AET) impact multiple water balance metrics that determine water available for runoff (WAfR). Overall, we found that WAfR had the strongest relationship with energy‐water asynchrony (adjusted r2 = 0.52) and that winter AET was correlated to total water year evapotranspiration but not to other water balance metrics. Stepwise regression analysis demonstrated that none of the tested mechanisms were strongly related to the Budyko‐type runoff anomaly (highest adjusted r2 = 0.21). We, therefore, conclude that WAfR from continental montane forests is most sensitive to the degree of energy‐water asynchrony that occurs. The results of this empirical study identify the physical mechanisms driving variability of WAfR in continental montane forests and are thus broadly relevant to the hydrologic management and modelling communities.

A snow-dwelling tropical butterfly? An unprecedented discovery of a new genus of the Pedaliodes clade in an extreme, high-altitude Andean environment (Lepidoptera: Nymphalidae: Satyrinae)

Abstract A new genus of satyrine butterflies, Nivaliodes gen. nov., is described for three species, all new: Nivaliodes negrobueno sp. nov., Nivaliodes viracocha sp. nov., and Nivaliodes puriq sp. nov. (Lepidoptera: Nymphalidae), with the support of molecular data and adult morphology. A target enrichment-based phylogeny indicates that Nivaliodes gen. nov. is sister to the genus Pherepedaliodes within an extremely diverse Pedaliodes clade of the predominantly Andean subtribe Pronophilina. Although an overwhelming majority of species of this group occur in tropical montane forests, N. negrobueno sp. nov. was discovered in a central Peruvian desert puna at some 4600–4800 m a.s.l., the highest elevation reported for any species of the Pronophilina. Individuals were observed overflying rocky slopes and resting directly on snow-covered surfaces, which is an exceptionally unusual behaviour among butterflies. The other two species of the new genus were found at lower elevations, some 3300–4200 m a.s.l., at the timberline and in puna grassland.

Highland forest dynamics across equatorial East Africa during the end of the African humid period

Tropical mountain ecosystems hold immense ecological and economic importance, yet they face disproportionate risks from shifting tropical climates. For example, present-day montane vegetation of East Africa is characterized by different plant species that grow in and are restricted to certain elevations due to environmental tolerances. As climate changes and temperature/rainfall zones move on mountains, these species must rapidly adjust their ranges or risk extinction.Paleoenvironmental records offer valuable insights into past climate and ecosystem dynamics, aiding predictions for ongoing climate change impacts. In particular, warming and wetting in tropical East Africa during the mid-Holocene resulted in both lowland and highland forest expansion. However, the relative impacts of rainfall and temperature change on montane ecosystems along with the influence of lowland forest expansion on montane communities is not completely understood. We use fossil pollen to study the vegetation changes in two lakes at different altitudes in the Rwenzori Mountains, Uganda: Lake Mahoma (Montane Forest belt) and Upper Kachope Lake (Afroalpine belt). Further, using the newly relaunched African Pollen Database and recent temperature reconstructions, we provide a regional synthesis of vegetation changes in the Rwenzori and then compare this with changes observed from other equatorial East African montane sites (particularly Mt Kenya).In the early to mid-Holocene in the Rwenzori Mountains, trees common today in lowland forests dominated, driven largely by warmer temperatures. After 4000 years ago (4ka), Afromontane forest trees along with grasses progressively replaced lowland trees. Not all sites experienced identical transitions. For instance, at Lake Rutundu on Mt Kenya at the same elevation as Lake Mahoma, bamboo expansion preceded Afromontane forest growth, likely influenced by variations in fire. Variance partitioning indicates that each site responded differently to changes in temperature and rainfall. Therefore, these site-specific ecological responses underscore the importance of considering biogeographic legacies as management strategies are developed, despite similarities in modern ecology.

The mountain giant rat of Borneo Sundamys infraluteus (Thomas) and its relations

The genus Sundamys comprises the species Sundamys muelleri, Müller‟s rat, which is widespread in the Sundaic biogeographical subregion, and three other taxa, the mountain giant rats of Borneo S. infraluteus, Java S. maxi and Sumatra S. atchinensis. Nine additional specimens of the Borneo Mountain giant rat are reported, and one field record, adding new locations to the known range of the species in the Crocker Range, notably the vicinity of Gn. Alab, and Gn. Lumaku, Sabah, and at Pa Raye, Kalimantan Utara. Measurements of the specimens, and the first from Gn. Mulu, Sarawak, are tabulated with previously published data. Trapping results indicate that this rat is confined to an altitudinal range, 900 – 2350 m, broadly corresponding with the limits of Lower Montane Forest, especially Oak-Laurel forests, and that it occurs both in pristine habitat and in disturbed forests. The topography of the uplands of northern Borneo provides connections between all locations except Mulu, which is surrounded by lowlands. A review of palaeoecological interpretations indicates that favourable habitat for this rat was more extensive in north-western Borneo in the terminal Pleistocene, but may have been more reduced than at present during warm episodes of the Holocene. Consideration of palaeo-environments in the Sundaic subregion suggests that there may have been no genetic contact between mountain giant rats of Sumatra and Borneo during the Quaternary. Although molecular evidence is lacking, it is reasonable to treat Sundamys infraluteus, S. atchinenesis and Sundamys maxi as distinct species, arising independently by vicariant evolution from a Pliocene ancestry.

Utilization of Forest Residues for Cellulose Extraction from Timber Species in the High Montane Forest of Chimborazo, Ecuador

The present study explored the extraction of cellulose from forest residues of four timber species, namely Cedrela montana Moritz ex Turcz, Buddleja incana Ruiz & Pav, Vallea stipularis L. f. and Myrsine andina (Mez) Pipoly, in the high montane forest of Chimborazo province, Ecuador, for the sustainable utilization of leaves, branches, and flowers. An alkaline extraction method was used on the residues without the need for prior degreasing. An ANOVA analysis was applied to evaluate significant differences in cellulose extraction yields among the species’ residues. The characterization techniques used were Fourier transform infrared spectroscopy (FTIR) and polarized light optical microscopy, which confirmed the successful extraction of cellulose with characteristics comparable to standard cotton cellulose and other traditional species. The results showed significant variations in cellulose yield among the species, with Vallea stipularis L. f achieving the highest yield of 80.83%. The crystallinity of the samples was clearly evidenced by the polarity of the light in the samples during microscopy, demonstrating that the residues can be a viable and sustainable source of cellulose, contributing to a circular economy and reducing the environmental impact of forest waste.

Sources and Sinks of N in Ecosystem Solutions Along the Water Path Through a Tropical Montane Forest in Ecuador Assessed With δ15N Values of Total Dissolved Nitrogen

AbstractThe globally increasing reactive N richness affects even remote ecosystems such as the tropical montane forests in Ecuador. We tested whether the δ15N values of total dissolved N (TDN), measured directly in solution with a TOC‐IRMS, can be used to help elucidate N sources and sinks along the water path and thus might be suitable for ecosystem monitoring. From 2013 to 2016, the δ15N values of TDN in bulk deposition showed the most pronounced temporal variation of all ecosystem solutions (δ15N values: 1.9–5.9‰). In throughfall (TF), TDN was on average 15N‐depleted (−1.8 ± s.d. 0.4‰) relative to rainfall (3.4 ± 0.9‰), resulting from net retention of isotopically heavy N, mainly as NH4+. Simultaneously, N‐isotopically light NO3−‐N and dissolved organic nitrogen (DON) with a δ15N value between NO3−‐N and NH4+‐N were leached from the canopy (leaves: −3.5 ± 0.5‰). The increasing δ15N values in the order, TF < stemflow (SF, 0.1 ± 0.6‰)< litter leachate (LL, 1.3 ± 0.7‰) concurred with an increasing DON contribution to TDN reflecting the δ15N value of the organic layer (1.9 ± 0.9‰). The lower δ15N value of the mineral soil solution at the 0.15 m soil depth (SS15, −1.5 ± 0.3‰) than in LL can be explained by the retention of DON and NH4+ and the addition of NO3− from mineralization and nitrification. The increasing δ15N values in the order, SS15 < SS30 (−0.6 ± 0.2‰) < streamflow (ST, 0.5 ± 0.6‰) suggested gaseous N losses because of increasing denitrification. There was no seasonality of the δ15N values. Our results demonstrate that the δ15N values of TDN in ecosystem solutions help identify N sources and sinks in forest ecosystems.

Checklist of the genus Dendrophorbium (Senecioneae: Compositae) in Peru including a new combination and a new species

The genus Dendrophorbium occurs in South America with one species known from the Caribbean. Despite recent advances in taxonomy and phylogeny, there is a still lack of molecular studies on the delimitation and identification of the genus. Dendrophorbium is known to occupy a wide range of ecosystems mostly restricted to subtropical montane forests and montane scrub vegetation of the central Andes. In this study, a review of the species occurring in Peru is presented, in addition to material examined at different herbaria. The new combination D. ligulatum which is separated from D. yalusay, and the new species, D. arachnoideum is described from the department of Huánuco in central Peru. The species are assessed using the standards of the IUCN and additional notes are given.

Conservation status of three rare endemic Phaseolus bean (Leguminosae, Phaseoleae) species of Costa Rica

We aimed at an inventory of all populations of wild Phaseolus (bean) species in Costa Rica, in relation to future plant breeding. During the field work in 2003–2018, three new and rare bean species (i.e. P. albicarminus, P. angucianae and P. hygrophilus) were found in different montane forest habitats which originally occupy a small acreage. Field observations, together with satellite images, indicate deforestation due to coffee plantations or pastures. To date only eleven populations of these species are known, distributed along the Talamanca and Fila Cruces mountainous ranges, making them endemic. Their narrow distribution, deforestation affecting the original habitats, and occurrence outside established and enforced protected areas motivate the assessment of their conservation status as ‘Endangered' (EN).

Root and biomass allocation traits predict changes in plant species and communities over four decades of global change.

Global change is affecting the distribution and population dynamics of plant species across the planet, leading to trends such as shifts in distribution toward the poles and to higher elevations. Yet, we poorly understand why individual species respond differently to warming and other environmental changes, or how the trait composition of communities responds. Here we ask two questions regarding plant species and community changes over 42 years of global change in a temperate montane forest in Québec, Canada: (1) How did the trait composition, alpha diversity, and beta diversity of understory vascular plant communities change between 1970 and 2010, a period over which the region experienced 1.5°C of warming and changes in nitrogen deposition? (2) Can traits predict shifts in species elevation and abundance over this time period? For 46 understory vascular species, we locally measured six aboveground traits, and for 36 of those (not including shrubs), we also measured five belowground traits. Collectively, they capture leading dimensions of phenotypic variation that are associated with climatic and resource niches. At the community level, the trait composition of high-elevation plots shifted, primarily for two root traits: specific root length decreased and rooting depth increased. The mean trait values of high-elevation plots shifted over time toward values initially associated with low-elevation plots. These changes led to trait homogenization across elevations. The community-level shifts in traits mirrored the taxonomic shifts reported elsewhere for this site. At the species level, two of the three traits predicting changes in species elevation and abundance were belowground traits (low mycorrhizal fraction and shallow rooting). These findings highlight the importance of root traits, which, along with leaf mass fraction, were associated with shifts in distribution and abundance over four decades. Community-level trait changes were largely similar across the elevational and temporal gradients. In contrast, traits typically associated with lower elevations at the community level did not predict differences among species in their shift in abundance or distribution, indicating a decoupling between species- and community-level responses. Overall, changes were consistent with some influence of both climate warming and increased nitrogen availability.