Diurnal fluctuation in streamflow during rainless periods provides valuable signals for the calibration of hydrological models and comprehension of hydrological processes. Previous research has primarily focused on this phenomenon in the context of single-factor analysis in the Mediterranean, Maritime, and Alpine climates of Middle Europe and Western US. This study is based on the Xitaizi Experimental Watershed (XEW), a typical mountainous headwater watershed situated in the semi-humid monsoon North China Plain. Seven years of high-frequency observation data were used to investigate the characteristics of streamflow diurnal fluctuation, its driving mechanisms, and the propagation pathways of these fluctuation signals through hillslope-riparian-stream. The results are as follows: (1) Streamflow exhibits diurnal fluctuation during rainless periods throughout the year, with variations up to 36% of the daily mean and distinct patterns in rainy (peak at night) and dry seasons (peak in the afternoon). Choosing the proper timing for daily discharge observation can reduce bias due to fluctuations. (2) In rainy seasons, the impact of discharge fluctuation is significant, particularly in years with lower discharge. Meanwhile, the soil moisture in both the hillslope and the riparian zone, and the groundwater level in the riparian zone exhibit a daytime decline and a nighttime rise. The groundwater level in the hillslope fluctuates with a lag time of approximately 6 to 9 hours. (3) Numerical simulations and cross-correlation analysis demonstrate that the dominant driving mechanism of diurnal fluctuations in rainy seasons is the diurnal evapotranspiration variation in the riparian zone, while the influence of aquifer hydraulic conductivity is insignificant.