A boronate functionalized monolithic capillary column was synthesized,characterized and utilized in the mode of both affinity and reversed phase chromatography.The polymer was prepared using 4-vinylphenylboronic acid as the functional monomer,pentaerythritol triacrylate as crosslinker,azobisisbutyronitrile as trigger,ethanediol and diethylene glycol as porogenator.In order to optimize the properties,the orthogonal experiment was designed by the DOE software.The optimum preparation conditions were monomer(4-vinylphenyl-boronic acid(VPBA),732 mg),cross-linking agent(pentaerythritol triacrylate(PETA),6 mg),trigger(azobisisobutyronitrile(AIBN),1 mg) and pore-foaming agent(diethylene glycol-ethanediol,2 ∶ 8,V/V).The column with the best proportion was characterized and applied in capillary high performance liquid chromatography(μHPLC) and pressurized capillary electrochromatography(pCEC).The results suggested the column persisted affinity chromatography character because the boronate group located at the stationary phase could specifically absorb compounds containing cis-dihydroxy functionality at alkaline conditions;because of the properties of PETA,the column also persist RP separation mechanism and separate different benzene series in 20 percent organic phase.