Brewster Angle Microscopy is a useful technique for the analysis of surface characteristics of films at the air‐water interface in a Langmuir monolayer trough due to its ability to obtain images while simultaneously collecting isothermal data. It provides an effective way of visualizing lipid domains, as well as lipid aggregation to form structures such as lipid rafts. The goal of this research was to construct a relatively low‐cost Brewster Angle Microscope (BAM) using LEGO™ Mindstorms, 3D printed components, a laser, a 10x microscope objective, and a CCD camera. This BAM was then used to obtain images of dipalmitoylphosphatidylcholine (DPPC) films and DPPC/Cholesterol films during a compression analysis. At the moment, we are working on programmatically quantifying the phase differences in lipid domains, as well as the surface density and thickness of films for analysis of lipid monolayers for drug delivery applications.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.