In order to accurately and effectively obtain the nitrogen content of tobacco leaves during the whole growth period, in the present study the field canopy spectrum of the three critical periods of tobacco rosette stage, vigorous growth stage and topping stage were used. The correlation analysis of field canopy spectrum, first derivative spectrum, hyperspectral parameters and vegetation index with the nitrogen content of tobacco leaves was carried out one by one, and the prediction model was established by multiple linear regression using the variables with the best correlation coefficient. Results showed that the first derivative spectrum, EVI II and green peak position had strong correlation, which is suitable for introducing multivariate equations as independent variables. Finally, the modeling determination coefficient (R2) was 0.66, RMSE was 0.40, and MAPE was 11%. The validation results showed that R2 was 0.73, RMSE was 0.38, and MAPE was 8.33%, which proved that this model could accurately predict the nitrogen content of tobacco leaves and could meet the requirements of large-scale statistical monitoring of tobacco quality indicators in the field. Bangladesh J. Bot. 52(2): 575-584, 2023 (June) Special
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