The temporal variation of glacier mass balance during recent years remains poorly known for the mountain glaciers in the inner Tibetan Plateau (ITP), due to the lack of field measurements. In this study, we retrieved the annual glacier mass balance of the Puruogangri ice field, which is the largest modern ice mass in the ITP, by differencing multi-temporal digital elevation models (DEMs) generated with five pairs of TanDEM-X bistatic interferometric synthetic aperture radar (InSAR) images acquired between April 2011 and January 2016. We compared the TanDEM-X DEMs in January and April 2012 to estimate the X-band radar penetration depth difference (0.61 ± 0.06 m), which enabled systematic bias correction in the estimation of annual glacier mass balance. The observed mass balance reveals a glacier mass gain of 0.44 ± 0.10 m w.e. yr−1 in 2011–12, which was followed by accelerated glacier mass loss in 2012–16. Glacier mass balances of −0.13 ± 0.03, −0.34 ± 0.06, and − 0.52 ± 0.10 m w.e. yr−1 were estimated for the periods of 2012–13, 2013–14, and 2014–16, respectively. This accelerating trend of glacier mass loss is likely caused by the continuous decrease in annual precipitation, according to the climate data recorded by adjacent meteorological stations. This study demonstrates that repeated bistatic InSAR measurement is a promising method for monitoring glacier elevation change and mass balance at regular intervals.