Mongolia Medical University Research Articles

Primary Extranodal Diffuse Large B-Cell Lymphoma in the Rituximab Era: a Single-Center Retrospective Analysis

To investigate the clinical features and prognostic factors of patients with primary extranodal diffuse large B-cell lymphoma (DLBCL) in the rituximab era. The continuous data of newly diagnosed DLBCL patients with complete case data and first-line treated with rituximab, cyclophosphamide, epirubicin, vincristine, prednisone (R-CHOP) or R-CHOP treatment admitted to the Affiliated Hospital of Inner Mongolia Medical University from January 2013 to November 2023 were retrospectively analyzed. The clinical and molecular immunological features and prognosis of extranodal DLBCL were analyzed, Logistics regression model was used to analyzed the influencing factors of patients prognosis. A total of 237 patients were enrolled, of which 54.4% (129 cases) were primary extranodal sources of DLBCL, and the most common extranodal sites were as follows: stomach (19.4%), colon (14.7%), tonsils (12.4%), skin/muscle (9.3%), central (7.7%), nasal/nasopharynx (6.2%), bone marrow (5.4%), testes (4.7%). The 3-year PFS and OS of DLBCL patients with extranodal involvement of bone marrow, central, liver, gastrointestinal or pulmonary origin were significantly lower than those of other patients with extranodal DLBCL of non-special site origin, and the difference was statistically significant (PFS: 65.2% vs 76.7%, P =0.008; OS: 82.6% vs 88.3%, P =0.04). Multivariate analysis showed that the prognostic factors affecting OS included NCCN-IPI score >3 (OR : 0.142, 95%CI : 0.041-0.495, P =0.002), non-germinal center source (OR : 2.675，95%CI :1.069-6.694，P =0.036), and DEL patients (OR : 0.327, 95%CI : 0.129-0.830, P =0.019). An NCCN-IPI score >3 was the only independent adverse prognostic factor for PFS (OR : 0.235, 95%CI : 0.116-0.474, P < 0.001). Patients with primary extranodal source DLBCL are more common in gastrointestinal involvement, and the overall prognosis is worse than that of patients with lymph node origin. NCCN-IPI score is an important independent adverse prognostic factor for predicting overall survival and progression-free survival in patients with primary extranodal diffuse large B-cell lymphoma.

Long-term outcomes of IVUS-guided and angiography-guided drug-eluting stent implantation for left main coronary artery disease: a retrospective consort study

BackgroundIn patients with unprotected left main coronary artery disease (ULMCAD), this study compared the long-term prognosis of drug-eluting stent insertion guided by intravascular ultrasonography (IVUS) vs. angiography.Patients and methodsThis retrospective consort investigation was performed in December 2021. This analysis included 199 patients who underwent IVUS-guided (IVUS group, n = 81) or angiography-guided (angiography group, n = 118) drug-eluting stent implantation at the Affiliated Hospital of Inner Mongolia Medical University between September 2013 and September 2018. Major adverse cardiac events (MACE) were defined as cardiovascular death, sudden cardiac death, myocardial infarction.ResultsThe IVUS group had considerably lower proportions of MACE within 1 year postoperatively (P = 0.002) and cardiac mortality within 3 years postoperatively (P = 0.018) compared to the angiography group. However, after adjusting for confounding variables, the hazard ratio for 3-year cardiac mortality was similar between the two groups (P = 0.28). In the IVUS group, there was considerably greater minimum lumen diameter (MLD) (P = 0.046), and reduced frequencies of target vessel restenosis (P < 0.050) and myocardial infarction (MI) (P = 0.024) compared to the angiography group. Cox regression analysis for 3-year cardiac mortality found that MSD was independently associated with low cardiac mortality (HR = 0.1, 95% CI: 0.01–14.92, P = 0.030).ConclusionIVUS-guided drug-eluting stent implantation may lead to better long-term prognosis in patients with ULMCAD, and MSD may be a predictor for lower cardiac mortality.

Meta-Analysis of Efficacy and Safety of Mongolian Medical Warm Acupuncture in the Treatment of Lumbar Disc Herniation

Objective: To systematically evaluate the safety and efficacy of Mongolian medical warm acupuncture in the treatment of lumbar disc herniation (LDH). Methods: CNKI, Wanfang, VIP, Pubmed, Embase, Cochrane Library, and other databases were searched. The randomized controlled trials (RCTs) on the treatment of LDH with Mongolian medical warm acupuncture were manually searched in the Chinese Journal of Ethnic Medicine, Chinese Journal of Mongolian Medicine, Journal of Inner Mongolia University for Nationalities, and Journal of Inner Mongolia Medical University. The search time limit was from January 2000 to October 2023. RevMan5.4 software was used to analyze the included and excluded literature. Results: A total of 8 RCTs involving 1,042 patients with LDH were included, with 551 patients in the observation group and 491 patients in the control group. The results of the meta-analysis showed that a total of 8 randomized controlled trials were included in the treatment of LDH with Mongolian medical warm acupuncture compared with simple acupuncture (RR = 1.18, 95% CI = [1.12, 1.23], P &lt; 0.00001). Conclusion: The total effective rate of Mongolian medical warm acupuncture for LDH is higher than that of simple acupuncture. However, due to the low quality of the literature included in this study, multi-dimensional, large sample size, and more rigorous clinical randomized trials are needed for further verification in the future.

Effect of venetoclax plus chemotherapy on treatment-naive acute myeloid leukemia patients with moderate to poor cytogenetic profiles and the combination's influence on the expression of proteins of the anti-apoptoic family

Objective: This was an open-label observational assessment aimed to evaluate whether venetoclax (VEN) plus chemotherapy could enhance the therapeutic benefits for treatment-naive acute myeloid leukemia (AML) patients with adverse cytogenetic profiles. Methods: A total of 38 adult patients (including 11 patients with moderate risk stratification and 27 patients with high risk stratification) who were treated at the Affiliated Hospital of Inner Mongolia Medical University from April 2019 to May 2022 were enrolled in this study. Patients were randomized into two cohorts according to the random number method to receive single intensive chemotherapy (18/38) alone or VEN+intensive chemotherapy (20/38), respectively. The chemotherapy cohort received 2 cycles of induction chemotherapy (idarbicin or daunorubicin plus cytarabine), followed by 6 cycles of consolidation chemotherapy (cytarabine), while the treatment for the VEN + chemotherapy cohort consisted of the same chemotherapy as above plus oral VEN. Heparinized bone marrow samples were obtained from patients at enrollment de novo and post chemotherapy. The expressions of MCL-1 and BCL-2 were detected by Western blot analysis. Results: Patients with VEN+chemotherapy showed an objective response rate (ORR) of 90.0% (18/20), compared with 55.6% (10/18, P=0.012) of the chemotherapy group. Meanwhile, the VEN + chemotherapy cohort gained more benefits in progression-free survival (PFS) and overall survival (OS) than the chemotherapy cohort (mean PFS: 27.1 months versus 17.9 months, P=0.038; mean OS: 32.2 months versus 21.3 months, P=0.004). For patients with moderate risk stratification, there were no differences in the ORR and PFS between the chemotherapy cohort and the VEN + chemotherapy cohort: the ORR was 80.0% (4/5) versus 100% (6/6, P=0.251), and the PFS was 27.9 months versus 32.0 months (P=0.582). Moreover, the ORR was 85.7% (12/14) for the VEN+chemotherapy cohort and 46.2% (6/13) for the chemotherapy cohort in the high risk profile (P=0.029). The PFS of the VEN+chemotherapy cohort was superior to the chemotherapy cohort in the high risk profile (mean PFS: 23.7 months versus 11.1 months, P=0.002). Meanwhile, in the chemotherapy cohort, there were no difference in the PFS between FAB-M5 patients and non-FAB-M5 patients; the mean PFS was 20.0 months versus 15.5 months (P=0.298) for the two groups. Nevertheless, FAB-M5 patients were inferior to non-FAB-M5 patients in PFS in the VEN + chemotherapy arm (mean PFS: 19.6 months versus 30.2 months, P=0.031). The most frequent grade 4 hematological toxicities (therapy related) were leukopenia and thrombopenia. Grade 3/4 hematological adverse events in patients treated with VEN+chemotherapy were not increased compared with those who received chemotherapy. Western blot showed VEN continuously decreased the expression of BCL-2 proteins in both FAB-M5 and non-FAB-M5 patients, but obviously increased the expression of MCL-1 proteins only in FAB-M5 patients. Conclusions: VEN combined with intensive chemotherapy have yielded high ORR and survival advantages for de novo AML patients with poor cytogenetics profiles. The high-expression of MCL-1 may drive resistance to VEN.

The Mechanism of Action and Experimental Verification of Narenmandula in the Treatment of Postmenopausal Osteoporosis.

Narenmandula is a classic ancient remedy in Inner Mongolia, historically used for gastrointestinal diseases. In recent decades, Inner Mongolia Medical University found that it has a significant effect in promoting fracture healing and increasing bone density, and has been used to treat postmenopausal osteoporosis (PMOP), but its mechanism is unclear. Identify the mechanism of action of Narenmandula for PMOP treatment. Network pharmacology, molecular docking and ovarian departing rat models were used to verify the relevant mechanism of Narenmandula in the treatment of PMOP. We confirmed that NRMDL prescription can improve OVX-induced bone loss, improve trabecular density, and relieve osteoporosis. Upon screening of network pharmacology, we obtained 238 overlapping genes of Narenmandula and PMOP, and analyzed AKT, IL1B, and IL6 as key genes by network topology. Among the 1143 target genes that interact with PMOP, 107 NRMDL active compounds correspond to 345 target genes and 238 overlapping genes. Network topology analysis showed the top 8 active ingredients, such as quercetin and kaempferol, and the top 20 key genes, such as AKT, IL1B, IL6, INS, JUN, STAT3, TNF, TP53, etc. Enrichment analysis revealed involvement of PI3K-Akt, HIF-1, FoxO, MAPK, and TNF signaling pathways. In addition, we found the most important active compounds bind tightly to core proteins, which were verified by molecular docking analysis. The AKT-related pathway had good binding energy, and the pathway was verified by cell and animal experiments. The potential mechanism and efficacy of Narenmandula against PMOP may be related to the PI3K-AKT pathway.

Expression and prognostic analysis of AEG-1, NKD-1and β-catenin in gastric cancer tissues

Objective: To investigate the expressions of AEG-1, NKD-1 and β-catenin in gastric cancer (GC), and analyze their relationship with clinicopathological features and prognosis and the correlation among the three proteins.Methods: Fifty GC tissues and 50 adjacent normal tissues of patients who underwent radical gastrectomy in the Third Affiliated Hospital of Inner Mongolia Medical University during December 2015 to June 2017 were collected. The expressions of AEG-1, NKD-1 and β-catenin were detected by immunohistochemical SP method and qRT-PCR. The relationship between positive expression and clinical features and survival time of patients was analyzed, and the correlation between the three was analyzed.Results: The positive expression rates of AEG-1 and β-catenin in gastric cancer were 74% (37/50), 60% (30/50), respectively, which was significantly higher than that of paracancer tissue (18% [9/50] and 26% [13/50]). The positive expression rate of NKD1 in gastric cancer tissues was 20% (10/50), which was significantly lower than that in adjacent tissues [88% (44/50)]; and the differences were statistically significant (p &lt; . 05). AEG-1 mRNA expression level (1.0148±0.0019) and β-catenin mRNA expression level (1.0000±0.0005) in gastric cancer tissues were significantly higher than that of AEG-1 mRNA expression level (1.0002±0.0036) and β-catenin expression level in adjacent tissues mRMA expression level (0.9984±0.0012), and the differences were statistically significant (p &lt; .05). The expression level of NKD1 mRNA in gastric cancer tissues (1.0008±0.0073) was significantly lower than that in adjacent tissues (1.0291±0.0041), and the differences were statistically significant (p &lt; .05). The positive expression of AEG-1, NKD1 and β-catenin in gastric cancer tissues was not related to age, sex or tumor size (p &gt; .05), but was related to differentiation degree, TNM stage and lymph node metastasis (p &lt; .05). In gastric cancer, there was a positive correlation between AEG-1 and β-catenin protein expression (r = .678, p &lt; .001), and a negative correlation between AEG-1 and NKD1 protein expression (r = -.323, p = .012). β-catenin expression was negatively correlated with NKD1 expression (r = -.347, p = .007). The survival time of patients with positive expression of AEG-1 and β-catenin was significantly shortened compared with those with negative expression (p &lt; .05), while the survival time of patients with positive expression of NKD1 was longer than that of patients with negative expression of NKD1 (p &lt; .05).Conclusion: The abnormal expression of AEG-1, NKD-1 and β-catenin is closely related with the occurrence, development, invasion, metastasis and prognosis of gastric cancer, which provide a therapeutic target and may become markers for prognosis evaluation.

The Application Value of Combined Detection of Serum IL-6, LDH, S100, NSE, and GFAP in the Early Diagnosis of Brain Damage Caused by Neonatal Asphyxia.

We aimed to investigate the correlation and clinical significance between a group of serum biomarkers and brain damage caused by neonatal asphyxia, and to provide sensitive and effective detection methods for early diagnosis and prognosis improvement. We enrolled neonates hospitalized in the neonatal department of The Affiliated Hospital of Inner Mongolia Medical University of China from June 2020 to June 2021 as the study subjects. The levels of inter-leukin-6 (IL-6), lactate dehydrogenase (LDH), S100 protein, neuron-specific enolase (NSE), and glial fibrillary acidic protein (GFAP) in serum samples were measured using electrochemiluminescence (ECL), enzyme-linked immunosorbent assay (ELISA) or rate method and the correlations between these serum biomarkers and the degree of neonatal asphyxia and brain damage were statistically analyzed using Spearman test. The levels of serum IL-6, LDH, S100, NSE, and GFAP in the neonatal asphyxia with brain damage group within 12 hours after birth were significantly higher than those in the neonatal asphyxia without brain damage group (all P<0.05). Additionally, these levels were positively correlated with the degree of asphyxia. The Area Under the Curve (AUC) of receiver operating characteristic (ROC) curves of IL-6 (0.8819), LDH (0.8108), S100 (0.8719), NSE (0.8719), and GFAP (0.8073) were revealed. The combined detection of serum marker levels can simultaneously reflect neuronal injury, glial cell injury, and inflammatory injury, improve the accuracy of diagnosis of neonatal asphyxia with brain damage, and enable the formulation of treatment strategies as early as possible to reduce the incidence of complications of brain damage.

Effect of dexmedetomidine on convalescence quality after general anesthesia and postoperative delirium, and on cognitive function in elderly patients undergoing lower limb surgery

Purpose: To investigate the effect of different doses of dexmedetomidine on the quality of postoperative recovery, incidence of postoperative delirium, and cognitive function in elderly patients undergoing lower limb surgery.&#x0D; Methods: A total of 112 patients who received treatment in the Department of Anesthesiology, Affiliated Hospital of Inner Mongolia Medical University, Hothot, China from January 2021 to January 2023 were divided into 3 groups, consisting of low-dose group (35 patients received 0.2 mg/kg), medium-dose group (39 patients received 0.4 mg/kg), and high-dose group (38 patients received 0.6 mg/kg). Parameters including, convalescence quality of general anesthesia, incidence of postoperative delirium, adverse reactions, Mini- mental State Examination (MMSE) scores, sedation effect (Ramsay), analgesia effect (visual analogue scale (VAS)), and stress indices, viz, norepinephrine (NE), epinephrine (E), and cortisol (COR) levels), were evaluated and compared.&#x0D; Results: There was no significant difference in tracheal extubation time, recovery time of spontaneous breathing, calling eye-opening time, or full awakening time among the three groups (p &gt; 0.05). However, MMSE score was significantly higher in low-dose group on days 1 and 3 after surgery (p &lt; 0.05) and VAS scores were significantly higher in low-dose group at 12 and 24 h after recovery compared to other groups (p &lt; 0.05). Ramsay score was significantly higher in high-dose group (p &lt; 0.05). Levels of NE, E, and COR were significantly lower in medium-dose group compared to other groups (p &lt; 0.05). Incidence of adverse reactions were significantly lower in low and medium-dose groups compared to high- dose group (p &lt; 0.05).&#x0D; Conclusion: Medium-dose dexmedetomidine demonstrates favorable sedative and analgesic effects with minimal impact on cognitive function and stress response in elderly patients undergoing lower limb surgery. Furthermore, it does not affect quality of postoperative recovery nor incidence of postoperative delirium. More large-scale, randomized controlled studies are needed to confirm these results.

Analysis of the efficacy and factors influencing survival of adjuvant radiotherapy for stage II-III biliary tract carcinoma

BackgroundTo determine the efficacy of adjuvant radiotherapy for stage II–III biliary tract carcinoma.MethodsWe retrospectively analyzed the data of 37 patients who underwent radical resection of biliary tract carcinomas at the Affiliated Hospital of Inner Mongolia Medical University between 2016 and 2020. We analyzed survival differences between patients who did (n = 17) and did not (n = 20) receive postoperative adjuvant radiotherapy by using Kaplan–Meier analysis. The log-rank test and Cox univariate analysis were used. The Cox proportional risk regression model was used for the multifactorial analysis of factors influencing prognosis.ResultsThe median survival time (28.9 vs. 14.5 months) and the 1-year (82.40% vs. 55.0%) and 2-year survival rates (58.8% vs. 25.0%) were significantly higher among patients who received adjuvant radiotherapy than among those who did not (χ2 = 6.381, p = 0.012). Multifactorial analysis showed that pathological tumor type (p = 0.004), disease stage (p = 0.021), and adjuvant radiotherapy (p = 0.001) were independent prognostic factors in biliary tract carcinoma. Subgroup analyses showed that compared to no radiotherapy, adjuvant radiotherapy significantly improved median survival time in patients with stage III disease (21.6 vs. 12.7 months; p = 0.017), positive margins (28.9 vs. 10.5 months; p = 0.012), and T3 or T4 tumors (26.8 vs. 16.8 months; p = 0.037).ConclusionAdjuvant radiotherapy significantly improved the survival of patients with biliary tract carcinoma, and is recommended especially for patients with stage III disease, positive surgical margins, or ≥ T3.

Digital measurement and clinical significance of proximal femur in the older people of Inner Mongolia population, China

ObjectiveThis study aimed to measure the parameters of the proximal femur in the older people of Inner Mongolia, China and understand the influence of age and gender so as to provide guidance for the design and improvement of prosthesis for total hip arthroplasty.MethodsA total of 236 patients who underwent CT angiography of lower limbs in the Department of Imaging, Affiliated Hospital of Inner Mongolia Medical University of China were collected. They were divided into 4 groups according to age: < 60 (group A), 60–69 (group B), 70–79 (group C), and > 80 years (group D). Four anatomical parameters, including femoral head diameter (FHD), femoral neck-shaft angle (FNSA), femoral offset (FO), femoral neck anteversion (FNA), were measured by Mimics 21.0. Comparisons were made between age groups of the same gender and between genders in the same age group to analyze the correlation of the 4 parameters of proximal femur with age and gender. In addition, the results of this study were compared with previous studies.ResultsThere were no significant differences in FHD and FO between age groups, indicating no correlation with age. FNSA and FNA were no significantly different between group C and group D in the same gender, whereas there were significant differences between other age groups and were negatively correlated with age. There were significant differences in FHD and FO between genders in the same age group, with the males being larger than the females. FNSA and FNA were no significant differences between genders in the same age group.ConclusionsFNSA and FNA decrease with age. FHD and FO were larger in males than in females in all age groups. Age and gender should be considered in the design of prosthesis.

Clinical analysis of cervical lymph node metastasis patterns and multivariate factors in differentiated thyroid carcinoma.

The incidence rate of thyroid cancer is rising rapidly in numerous parts of the world, but the mortality rate is relatively stable or even declining. The aim of the present study was to analyze the risk factors of cervical lymph node metastasis (LNM) in differentiated thyroid carcinoma (DTC). The clinical data of 846 patients with DTC were collected from the Department of General Surgery of Chifeng Municipal Hospital of Inner Mongolia Medical University (Chifeng, China) from June 2018 to June 2022. The relationship between central LNM (CLNM) and lateral LNM (LLNM) was explored in terms of sex, age, tumor diameter, multifocality, capsular invasion and Hashimoto's thyroiditis. It was revealed that male sex, age <35 years, tumor size >1 cm, multifocality and capsular invasion were associated with CLNM and LLNM (P<0.001), while there was no relationship between Hashimoto's thyroiditis, CLNM and LLNM (P>0.05). The number of positive lymph nodes in CLNM dissection, accounting for ≥50% of the total number of lymph nodes dissected, was significantly associated with LLNM (P<0.0001). In conclusion, there was no correlation between Hashimoto's thyroiditis and CLNM and LLNM. The present study revealed that patients with the characteristics of sex, age <35 years, tumor size >1 cm, multifocality and capsular invasion were associated with cervical LNM. The proportion of the number of central lymph node metastases to the total number of lymph nodes cleared during surgery is more than or equal to 50%, indicating a susceptibility to external cervical lymph node metastasis. The results of multivariate logistic analysis showed that male sex, multifocality, capsular invasion and CLNM were risk factors for LLNM, and age was a protective factor for LLNM in DTC.

College students still maintain the traditional Chinese concept of love

A cross-sectional census was conducted on all students at the campus of Inner Mongolia Medical University using a self-administered questionnaire. This study aimed to investigate their love from psychological pressure, behavioral characteristics, social cognition, etc. Our results show that all students cultivate feelings and become lifelong partners as their motivation for love. Among the population with various demographic characteristics, the prevalence of love among disadvantaged groups is low; that is, women are lower than men, and those from rural are lower than those from cities. After balancing areas and gender, mental working families have a high prevalence of love. Instead, students whose parents' high expectations pressure their children to have a high love prevalence. This is due to China's specific historical perspective. Our results suggested that although China has been deeply integrated with the world and has more frequent ideological and cultural exchanges, college students still inherit China's traditional outlook on love.

"Application Progress of Traditional Chinese Medicine Therapy in the Treatment of Depression"

Psychology and Psychotherapy: Research Studys Application Progress of Traditional Chinese Medicine Therapy in the Treatment of Depression Chen Li, He Han, Ran Dong and Yajing Hou* Department of Traditional Chinese Medicine, Inner Mongolia Medical University, Hohhot, China *Corresponding author: Yajing Hou, Department of Traditional Chinese Medicine, Inner Mongolia Medical University, Hohhot, China Submission: January 23, 2023Published: February 21, 2023 DOI: 10.31031/PPRS.2023.06.000637 ISSN 2639-0612Volume6 Issue3

Overexpression of S100A1 in Osteosarcoma Inhibits Tumor Proliferation and Progression.

Osteosarcoma is the most common primary malignant tumor of bone. Abnormal expression of S100A1 protein is closely related to the occurrence and development of malignant tumors. However, S100A1 in osteosarcoma has not been studied. All osteosarcoma tissues were collected from patients who received surgical therapy at the Affiliated Hospital of Inner Mongolia Medical University, China in 2020. QRT-PCR and western blot assays were used to detect the expression of S100A1 in osteosarcoma tissues and cells. The negative effect of S100A1 on osteosarcoma cell growth was confirmed by vitro and vivo experiments. S100A1 inhibited the growth of osteosarcoma cells in vitro. Overexpression of S100A1 may inhibit the proliferation of osteosarcoma cells by preventing the activation of AKT signaling pathway by western blot assay. Finally, animal experiments confirmed that overexpression of S100A1 could inhibit the proliferation of osteosarcoma cells. Overexpression of S100A1 obtained better survival benefit in mice. Our findings provided a new insight to the treatment of osteosarcoma. It also raised the possibility that S100A1 could be used in targeted therapies for osteosarcoma.

Clinical significance of R-TEG in severe burn patients with coagulation dysfunction

IntroductionThe aim of this study was to retrospectively analyze Thrombelastography (TEG) data of severe burn patients to provide a clinical basis for timely diagnosis and treatment of coagulation dysfunction. MethodsThe present study comprised burn patients with full thickness TBSA ≥ 60%. The patients included in the study were admitted to the Third Affiliated Hospital of Inner Mongolia Medical University between March 2019 and March 2022 and died within 10 days. Patient demographic and clinical data, including abbreviated burn severity index (ABSI) score, full thickness and overall total surface burn area (TBSA), injury cause, International Society on Thrombosis and Hemostasis (ISTH) score, were retrieved from the electronic medical record system. TEG data (including ACT, K, α, MA and LY30), platelet count (PLT), mean platelet volume (MPV) and platelet distribution width (PDW) data were obtained from the records of included patients for analysis. ResultsA total of 9 patients were enrolled. The average burn area was 90.0% TBSA and the full-thickness TBSA was 72.0%. The results showed that α, MA and PLT count values were significantly lower relative to those at obtained throughout admission period (all p < 0.05). PDW and MPV were significantly higher compared with the values at admission (all p < 0.05). ACT time was significantly longer from day 2 after severe burn compared with the ACT time at admission (all p < 0.05). LY30 value from day 3 after severe burn was significantly higher compared with the value at admission (p < 0.05). One patient was diagnosed with diffuse intravascular coagulation (DIC) on admission, whereas eight patients were diagnosed with DIC on the day of death. ConclusionCoagulation dysfunction after severe burn is mainly characterized by procoagulant disorders and hyperfibrinolysis, which can be timely detected by TEG. Coagulation after severe burn exhibits a gradual aggravation, and can lead to death of patients.

Migraines in medical students from Inner Mongolia University

Aims: This study aimed to evaluate the prevalence of migraines and related factors.&#x0D; Methods: The survey employed was a self-administered questionnaire regarding various migraine symptoms and related factors administered to medical students attending Inner Mongolia Medical College in China. We calculate migraine prevalence and evaluate migraine-related factors. Migraine prevalence based on related factors and according to gender were compared using χ2 tests. The proportion of other symptoms related to migraines was also calculated.&#x0D; Results: 17.2% of surveyed students had experienced migraines. The migraines prevalence among students who used the computer for over three hours was nearly 1.5 times higher than for those who used it for less than one hour. Of those students who used a computer for more than 3 hours, migraine prevalence among female students was approximately 1.5 times greater than for males. The migraine prevalence among students with poor sleep was nearly two times higher than among those with good sleep. The migraine prevalence among students with anxiety was approximately 2.5 times higher relative to those who experienced no anxiety. The most common symptom complaint associated with migraines (&gt;97%) included limited ability to study and restricted daily activities.&#x0D; Conclusions: Although the migraine prevalence in our study was moderate, it led to serious limitations in students’ study time and daily activities. In addition to measures to relieve anxiety and improve sleep quality, stricter control of computer use will be necessary, especially in female. Taken together, our study provides better insight regarding medical students’ migraine-related problems.

Effect of combined treatment with benazepril and spironolactone on diabetic nephropathy and serum levels of IL-6, CRP and TNF-α

Purpose: To study the therapeutic effect of a combination of benazepril and spironolactone on diabetic nephropathy patients, and also to determine the influence of the combined treatment on serum IL-6, CRP and TNF-α.Methods: 100 diabetic nephropathy patients admitted to The Affiliated Hospital of Inner Mongolia Medical University from April 2019 to October 2020 were randomly chosen and divided into groups E and F by drawing lots (n = 50 each). Group E received benazepril, while group F received a combined treatment of benazepril and spironolactone. Therapeutic efficacy, incidence of adverse drug reactions, and renal function after treatment, as well as IL-6, CRP and TNF-α levels in serum pre- and posttreatment, were determined. Fasting blood glucose (FBG) levels were also measured pre-treatment, and at 7 days and 14 days post-exposure to drugs.Results: Therapeutic efficacy and renal functions were significantly better in group F than in group E, while post-treatment incidence of adverse drug reactions, and expression levels of CRP, IL-6, and TNF-α levels were significantly reduced in group F, relative to group E (p &lt; 0.05). Following treatment, the inflammatory factor levels were decreased in both groups.Conclusion: The combination of benazepril and spironolactone produces higher treatment effect in diabetic nephropathy patients than those that received benazepril only. The combined treatment is recommended in the management of diabetic nephropathy patients.

Functional annotation and enrichment analysis of differentially expressed serum proteins in patients with type 2 diabetes after dapagliflozin.

BACKGROUNDOnly 50% of patients with type 2 diabetes mellitus (T2DM) can control their blood glucose levels. Dapagliflozin is a selective inhibitor of sodium-glucose co-transporter 2 (SGLT-2) that improves the insulin sensitivity of the liver and peripheral tissues. Many studies confirmed that SGLT2 inhibitors reduce blood glucose and have multiple beneficial effects such as weight loss, lipid regulation, and kidney protection. Nevertheless, the mechanisms of the renal and cardiovascular protective effects of dapagliflozin from the perspective of differentially expressed proteins in the serum of T2DM patients have not been intensively explored so far.AIMTo identify differentially expressed proteins associated with dapagliflozin treatment in patients with T2DM.METHODSTwenty T2DM patients [hemoglobin A1c (HbA1c) 7.0%-10.0%] were enrolled at The Affiliated Hospital of Inner Mongolia Medical University between January 1, 2017 and December 1, 2018. They received dapagliflozin (10 mg/d) for 3 mo, and the HbA1c < 7.0% target was achieved. The changes in clinical indexes were compared before and after treatments. Label-free quantitative proteomics was used to identify differentially expressed proteins using the serum samples of five patients. The identified differentially expressed proteins were analyzed using various bioinformatics tools. RESULTSDapagliflozin significantly improved the clinical manifestation of the patients. There were 18 downregulated proteins and one upregulated protein in the serum samples of patients after dapagliflozin administration. Bioinformatics analyses, including subcellular localization, EuKaryotic Orthologous Groups, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes annotations, were used to profile the biological characteristics of the 19 differentially expressed proteins. Based on the literature and function enrichment analysis, two downregulated proteins, myeloperoxidase (MPO) and alpha II B integrin (ITGA2B), and one upregulated protein, podocalyxin (PCX), were selected for enzyme linked immunosorbent assay validation. These validated differentially expressed proteins had multiple correlations with clinical indexes, including HbAc1 and fasting C-peptide. CONCLUSIONDapagliflozin has hypoglycemic effects and regulates the serum expressions of MPO, ITGA2B, and PCX, possibly contributing to the effects of dapagliflozin on oxidative stress, insulin resistance, and lipid metabolism.

Development of prognostic predictive model with neutrophil-lymphocyte ratio (NLR) in patients with gastric signet ring carcinoma.

The risk factors have not been well-defined for prognosis in gastric signet ring cell carcinoma (GSRC) patients. This study is designed to prognosticate survival in GSRC patients by establishing and verifying a predictive model with neutrophil–lymphocyte ratio (NLR).A total of 147 GSRC patients from Department of Surgical Oncology, Neimenggu Baogang Hospital, Inner Mongolia Medical University were retrospectively reviewed. A predictive model was established using Cox proportional hazards. The performance of the model was evaluated by ROC curves.In present study, we found that overall survival (OS) (P < .001, Fig. 1A) and tumor recurrence rate (P = .036, Fig. 1B) in the NLR ≤ 2.8 group were significantly better than those in the NLR > 2.8 group. These results showed that NLR ≤ 2.8 was significant prognostic factor related with both OS and tumor recurrence in patients with GSRC. After adjusting for competing risk factors, NLR ≤ 2.8 (hazard ratio [HR]: 2.625, 95% confidence interval [CI]: 1.505–5.3166, P = .003), tumor size (HR: 3.024, 95% CI: 1.521–4.186, P = .005), and tumor metastasis (HR: 3.303, 95% CI: 1.25–4.525, P = .012) remained independent predictors of tumor recurrence rate and OS. Our results showed that comparing with the model without NLR (area under ROC curve: 0.798), the model with NLR (area under ROC curve: 0.826) had significant better predictive power than the model without NLR, which further confirmed the value of NLR in predicting prognosis of patients with GSRC.In conclusion, a high NLR value independently predicts poor survival in patients with GSRC after surgery. The NLR may help oncologists evaluate outcomes of patients received surgical resection and chemotherapy in order to choose alternative therapies for patients with high NLR value.

Correlation between Lactobacillus and expression of E-cadherin, β-catenin, N-cadherin, and Vimentin in postmenopausal cervical lesions.

To detect the correlation between Lactobacillus vaginalis and the expression of epithelial-mesenchymal transition (EMT)-related factors, E-cadherin, β-catenin, N-cadherin, and Vimentin, in postmenopausal cervical squamous intraepithelial lesions (SILs) and cervical squamous cell carcinoma (SCC), and to explore the possible mechanism. From January 2016 to January 2020, 30 postmenopausal patients with low-grade SIL (LSIL), 18 patients with high-grade SIL (HSIL), and 30 patients with SCC who underwent colposcopy biopsy in the Outpatient Department of the First Affiliated Hospital of Inner Mongolia Medical University were selected as the experimental group, and 30 postmenopausal normal women were selected as the control group. The expression of 16SrRNA of Lactobacillus vaginalis in each group was determined by the 16S third-generation full-length amplification sequencing technique. The mRNA expression levels of E-cadherin, β-catenin, N-cadherin, and Vimentin were detected by quantitative real-time polymerase chain reaction (qPCR). The correlation between the 16SrRNA expression level of Lactobacillus vaginalis and the mRNA expression level of the EMT-related proteins was compared among all groups. (I) The progression of postmenopausal cervical SILs to cervical SCC was significantly positively correlated with age, number of pregnancies, smoking, pH value, positive rate of HPV16, and negatively correlated with total Lactobacillus 16SrRNA expression (P<0.0001). (II) The level of vaginal microbiota in postmenopausal women showed that Lactobacillus iners was dominant. With the progression of the disease, the expression levels of 16SrRNA in Lactobacillus iners and Lactobacillus total vagina decreased gradually, and the differences were statistically significant (P<0.05). (III) With the disease progresses. The expression of total Lactobacillus 16SrRNA was positively correlated with the mRNA expression of β-catenin and E-cadherin (r>0; P<0.05), and negatively correlated with the mRNA expression of Vimentin and N-cadherin (r<0; P<0.05). In postmenopausal women, Lactobacillus vaginalis interacts with HPV and is associated with the occurrence of EMT, promoting the development of cervical lesions.