This research leads to reach the effects of the off-design operation on the aerodynamic performance, boundary layer behavior, and associated loss occurring in a high deflection industrial turbine blade. Numerical simulations, proved opposed to experimental results, are used to achieve the detailed flow field of the cascade, incidence angels ranging from −15° to +10° at inlet chord Reynolds number varied from 9.63E+04 to 2.24E+05. Boundary layer specifications covering thickness, displacement thickness, and momentum thickness are achieved in the 15 points on the suction side and talk about detail on the authority of numerical simulations in various flow conditions. Results indicate that profile loss is forcefully increased by the rising up the incidence angle mostly in the positive range. Studying the effect of incidence angle on the boundary layer behavior indicates that as incidence angle varies from negative to positive, all three characteristics of the boundary layer increase gradually.