Mollisol Soil Research Articles

Soil erosion‐induced decline in aggregate stability and soil organic carbon reduces aggregate‐associated microbial diversity and multifunctionality of agricultural slope in the Mollisol region

AbstractSoil erosion has become a serious ecological problem hindering the agricultural and economic development in Northeast China. Soil aggregates are basic units that regulate soil fertility and microbial activity. However, there have been few reports on the role of soil erosion in regulating the microbial diversity, multiple ecosystem services, and functions (multifunctionality) of soil aggregates. In this study, we investigated the effects of soil erosion on microbial diversity and the activities of various extracellular enzymes associated with soil aggregates by using Mollisol soil in Northeastern China at different positions of a slope, and how soil erosion affects soil multifunctionality indexes obtained by normalized calculation of soil enzyme activities. The results showed that soil erosion could reduce the physicochemical properties, soil enzyme activity, and microbial diversity of the aggregates, resulting in accumulation of aggregate nutrients and increase in microbial diversity and enzyme activity in the sedimentation area. Compared with smaller soil aggregates, larger soil aggregates had higher nutrient content, enzyme activity, and microbial diversity. However, with the intensification of soil erosion, the differences in microbial diversity and soil enzyme activities among different sizes of aggregates were reduced. Soil erosion also significantly reduced soil multifunctionality (p < 0.001). The multifunctionality of 2000–250 μm, 250–53 μm, and <53 μm aggregates respectively declined from 0.52, 0.20, and 0.08 (no erosion sites) to −0.17, −0.66, and −0.77 (heavy erosion sites). Additionally, microbial diversity was found to have a linear positive correlation with soil multifunctionality (R2 = 0.23, p < 0.01). Structural equation modeling and random forest analysis revealed that soil organic carbon and aggregate water stability are main predictors of soil multifunctionality. Furthermore, soil physicochemical properties had a greater influence on soil multifunctionality than microbial diversity. Collectively, our results demonstrate that soil erosion negatively affects the structure, resource effectiveness, and microbial activity of soil aggregates, thereby reducing to differential soil multifunctionality.

Yield and fruit quality traits of rambutan cultivars grafted onto three rootstocks and grown in a Mollisol soil in Puerto Rico

With increasing ethnic diversity and demand for healthy and more varied foods, globalization has opened a window of opportunity for new tropical fruits, including rambutan (Nephelium lappaceum), to be offered to consumers in the Western Hemisphere. The lack of formal experimentation on rambutan performance using various scion/rootstock combinations prompted a study, conducted in Santa Isabel, Puerto Rico, using a randomized complete block design to assess the yield and fruit quality of three cultivars (‘Gula Batu’, ‘Jitlee’, ‘R-162’) when grafted onto three different rootstocks (‘Binjai’, ‘Gula Batu’, ‘R-134’) in an alkaline Mollisol soil. From 2011 to 2015, there was an increase in the number and yield of fruit for cultivar/rootstock treatments. Regardless of rootstock, ‘R-162’ and ‘Jitlee’ had a higher number of fruit and yield than ‘Gula Batu’. The cultivars produced significantly more fruit and had significantly higher yields when ‘Gula Batu’ was used as a rootstock. Cultivar/rootstock treatments did not have a significant effect on individual fruit weight which averaged 32 g. Cultivar ‘R-162’ grafted onto ‘Gula Batu’ had a higher concentration of soluble solids, but it was not significantly different than the rest of the treatments except for ‘Gula Batu’ grafted onto ‘Binjai’ and ‘Gula Batu’ grafted onto ‘R-134’, which had significantly lower soluble solids concentration. Individual fruit weight and rind weight did not vary among cultivar/rootstock treatments, and pulp weight varied very little. Seed weight differences were mainly associated with cultivar ‘Gula Batu’. This study confirms previous work by the author showing ‘R-162’ as a highly productive cultivar and, for the first time, shows that ‘Gula Batu’ is a superior rootstock, performing very well under alkaline soil conditions.

Characterization of dissolved organic matter distribution in forestland and farmland of mollisol based on untargeted metabolomics

Mollisol soil is a major contributor to food production. Clarification of the molecular characteristics of dissolved organic matter (DOM) will contribute to the overall understanding and management of mollisol soil. However, the complexity of DOM poses a challenge to understanding its molecular characteristics. In this study, we investigated the molecular characteristics of DOM (< 1 000 Da) in mollisol soils with different soil use patterns (forestland and dryland) based on untargeted metabolomics. Here, we confirmed the feasibility of untargeted metabolomics for the molecular characterization of DOM in mollisol soils. DOM in forestland is mainly derived from plant metabolites, and DOM can perform more biological functions. However, DOM in dryland has complex composition and has powerful co-occurrence network interactions due to human activities. Water has better extraction efficiency for polar DOM, while organic reagents can efficiently extract lipid-like DOM, but the polarity of the extractant has less influence on the DOM than the soil physicochemical properties. Meanwhile, 14-dihydroxyzeatin screened based on metabolomics can be used as a potential indicator for corn land. Therefore, untargeted metabolomics can be an effective method to characterize the DOM molecules of mollisol soil, which provides new insights for management of mollisol soil and sustainable agricultural development.

Performance of atrazine adsorption behavior and microbial community structure in Mollisol aggregate fraction

Owing to complex pore systems and chemical substances, soil aggregates provide a spatially heterogeneous microenvironment for adsorption capacity and microbial survival. As the widely used pesticide in farmlands, atrazine environmental behavior is not well known at the aggregate scale. In this study, Mollisol soil samples were sieved into four aggregate-size classes: large macroaggregates (>2 mm, LMa), small macroaggregates (1–2 mm, SMa), microaggregates (0.25–1 mm, Mia) and primary particles (<0.25 mm, P). The pore characteristics of each aggregate fraction was visualized by non-invasive X-ray three-dimensional microscopic computed tomography (3D-CT) combined with pore network extraction. The adsorption kinetics of atrazine in each aggregate-size fraction can be described well by a pseudo-second-order kinetic model. The adsorption isothermal process of atrazine can be better fitted by the Langmuir isotherm model than Freundlich isotherm model. There was an obvious linear correlation between the maximum atrazine adsorption capacity and aggregate SOC content as well as TN. In addition, the abundance of bacteria, actinomycetes and anaerobic bacteria in P was totally higher than those in SMa and Mia. Although pH is strongly linked to the bacterial community in the aggregate fraction, aggregate particle size explained 18 % for shaping the microbial community. Therefore, chemical properties and pore characteristics of each soil aggregate fraction both contributed to performance of atrazine adsorption behavior and microbial community.

Chemodiversity of soil organic matters determines biodegradation of polychlorinated biphenyls by a graphene oxide-assisted bacterial agent

A promising strategy for degrading persistent organic pollutants (POPs) in soil is amendment with nanomaterial-assisted functional bacteria. However, the influence of soil organic matter chemodiversity on the performance of nanomaterial-assisted bacterial agents remains unclear. Herein, different types of soil (Mollisol soil, MS; Ultisol soil, US; and Inceptisol soil, IS) were inoculated with a graphene oxide (GO)-assisted bacterial agent (Bradyrhizobium diazoefficiens USDA 110, B. diazoefficiens USDA 110) to investigate the association between soil organic matter chemodiversity and stimulation of polychlorinated biphenyl (PCB) degradation. Results indicated that the high-aromatic solid organic matter (SOM) inhibited PCB bioavailability, and lignin-dominant dissolved organic matter (DOM) with high biotransformation potential was a favored substrate for all PCB degraders, which led to no stimulation of PCB degradation in MS. Differently, high-aliphatic SOM in US and IS promoted PCB bioavailability. The high/low biotransformation potential of multiple DOM components (e.g., lignin, condensed hydrocarbon, unsaturated hydrocarbon, etc.) in US/IS further resulted to the enhanced PCB degradation by B. diazoefficiens USDA 110 (up to 30.34%) /all PCB degraders (up to 17.65%), respectively. Overall, the category and biotransformation potential of DOM components and the aromaticity of SOM collaboratively determine the stimulation of GO-assisted bacterial agent on PCB degradation.

Distribution of Four Vole Species through the Barn Owl Tyto alba Diet Spectrum: Pattern Responses to Environmental Gradients in Intensive Agroecosystems of Central Greece.

Voles are the most common vertebrate pests in European agriculture. Identifying their distribution and abundance patterns provides valuable information for future management. Barn Owl diet analysis is one of the optimum methods used to record small mammal distribution patterns on large spatial scales. From 2003 to 2005, a total of 10,065 Barn Owl pellets were collected and analyzed from 31 breeding sites in the largest agroecosystem in Greece, the Thessaly plains. A total of 29,061 prey items were identified, offering deep insight into small mammal distribution, specifically voles. Four discrete vole species (Harting's vole Microtus hartingi, East European vole Microtus levis, Thomas's pine vole Microtus thomasi, and Grey dwarf hamster Cricetulus migratorius) comprised 40.5% (11,770 vole prey items) of the total Barn Owl prey intake. The presence and abundance of the voles varied according to underlying environmental gradients, with soil texture and type playing a major role. M. levis showed no significant attachments to gradients, other than a mild increase in Mollisol soils. It was syntopic in all sites with M. hartingi, which was the dominant and most abundant small mammal species, preferring non-arable cultivated land, natural grasslands, set-aside fields, and fallow land. M. thomasi was strictly present in western Thessaly and strongly associated with a sandy-clay soil texture and Alfisol soils. C. migratorius was the least represented vole (162 items), exclusively present in eastern Thessaly and demonstrating a stronger association with cereals, Mollisol soils, and an argillaceous-clay soil texture. This is the first study in Greece at such a large spatial scale, offering insights for pest rodents' distribution in intensive agroecosystems and their response to environmental gradients including soil parameters.

Incorporation of maize crop residue maintains soybean yield through the stimulation of nitrogen fixation rather than residue-derived nitrogen in Mollisols

Crop residue amendment to soil is recommended as an effective management practice to return nutrients, especially in the maize-soybean rotation system where large amounts of maize residues are produced. Quantifying the utilisation of maize-residue N by the subsequent soybean crop is essential for optimising the N fertilisation strategy for sustainable production. However, whether and how maize residue amendment alters N acquisition in soybean plants are largely unknown. It was hypothesised that maize residue would supply N and enhance N2 fixation to meet the N requirements of subsequent soybeans. Three treatments, namely: 1) chemical fertiliser (55.2, 35.2 and 22.4 kg ha−1 of N, P and K, respectively), 2) maize residue (8 t ha−1), and 3) non-fertiliser were applied in a maize-soybean rotation system in a Mollisol soil. It was demonstrated that soybean seed yield in the maize-residue treatment was the same as that in the chemical fertiliser treatment, with 2.9 vs. 3.2 t ha−1 in 2014, 2.7 vs. 2.6 t ha−1 in 2016, and 3.0 vs. 3.1 t ha−1 in 2018. A follow-up pot experiment using 15N-labelled residue indicated that the residue-derived N accounted for 0.5 % of the total N in soybean seeds and the proportion of symbiotically fixed N reached 82 %. The amount of fixed N during the pod-filling period in the residue treatment was 0.66 g plant-1, which was 49 % and 41 % higher than those in the chemical fertiliser and non-fertiliser treatments, respectively. The stimulation of N2 fixation was associated with an increase in fixed N per nodule and the enrichment of diazotrophs in the rhizosphere of soybean. With maize residue amendment, the increased N2-fixing capability of nodules during the reproductive period, rather than residue-derived N, fulfilled the N demand for maintaining seed yield of soybean. In the maize-soybean rotation system, maize residue amendment would facilitate the N2 fixation to partly substitute for N fertiliser for soybean production in Mollisols.

Microbial metabolism and necromass mediated fertilization effect on soil organic carbon after long-term community incubation in different climates.

Understanding the effects of changing climate and long-term human activities on soil organic carbon (SOC) and the mediating roles of microorganisms is critical to maintain soil C stability in agricultural ecosystem. Here, we took samples from a long-term soil transplantation experiment, in which large transects of Mollisol soil in a cold temperate region were translocated to warm temperate and mid-subtropical regions to simulate different climate conditions, with a fertilization treatment on top. This study aimed to understand fertilization effect on SOC and the role of soil microorganisms featured after long-term community incubation in warm climates. After 12 years of soil transplantation, fertilization led to less reduction of SOC, in which aromatic C increased and the consumption of O-alkyl C and carbonyl C decreased. Soil live microbes were analyzed using propidium monoazide to remove DNAs from dead cells, and their network modulization explained 60.4% of variations in soil labile C. Single-cell Raman spectroscopy combined with D2O isotope labeling indicated a higher metabolic activity of live microbes to use easily degradable C after soil transplantation. Compared with non-fertilization, there was a significant decrease in soil α- and β-glucosidase and delay on microbial growth with fertilization in warmer climate. Moreover, fertilization significantly increased microbial necromass as indicated by amino sugar content, and its contribution to soil resistant C reached 22.3%. This study evidentially highlights the substantial contribution of soil microbial metabolism and necromass to refractory C of SOC with addition of nutrients in the long-term.

Planting season and date of harvest effect on biomass production and nitrogen contribution of Crotalaria juncea L. 'Tropic Sun' in southwestern

Sunn hemp (Crotalaria juncea L.) is a fast-growing, multipurpose legume of high N2 fixation ability, and an option for use in improving the fertility of degraded tropical soils. The objective of this study was to compare the effects of two planting seasons and three harvest dates on biomass yield (DMY) and N accumulation in the vegetative material of sunn hemp Tropic Sun'. Sunn hemp was seeded at the Lajas Agricultural Experimental Substation of the University of Puerto Rico in a Mollisol soil (San Antón Series) at a density of 7 kg/ha in May and October, and harvested at 84, 121, and 177 days after planting (DAP). The 177 DAP date proved to be not feasible because of previous senescence of the plants from the October planting; therefore, it was excluded from the analysis. Planting in May rather than October and harvesting at 121 DAP rather than 84 DAP resulted in higher DMY (kg/ha) (7,248 vs. 5,975 and 7,946 vs. 5,277, respectively). However, only the difference between planting seasons was significant (P &lt; 0.05). The same tendencies were observed in N accumulation (kg/ha), means of 126.55 vs. 105.09 and 120.65 vs. 110.99, respectively, but without differences at P = 0.05. No interactions (P &gt; 0.05) were detected between seasons of planting and date of harvest in these two dependent variables. Differences (P &lt; 0.05) were found in N content of plant tissue, the upper canopy mean surpassing that of the lower canopy (2.12 vs. 1.00%). The highest crude protein content of 16.05% was found in the upper canopy for the May planting. These results suggest that sunn hemp has good potential as a cover crop and green manure in the Lajas region, and that summer planting is preferable.

Greater variation of bacterial community structure in soybean- than maize-grown Mollisol soils in responses to seven-year elevated CO2 and temperature

Changes in rhizodeposits of crops under elevated CO2 (eCO2) and elevated temperature (eT) may substantially impact on soil microbial community, which in turn affects soil carbon and nutrient cycling. However, the responses of soil bacterial community to long-term eCO2 and eT are not fully understood. A seven-year field experiment using open-top chambers was carried out with soybean (Glycine max L. Merr.) and maize (Zea mays L.) grown in a Mollisol soil under ambient CO2 (380 ppm), eT (2.1 °C increase in air temperature) and eTeCO2 (elevated temperature plus elevated CO2, 2.1 °C increase in air temperature and 700 ppm CO2). Soil DNA was extracted for Illumina MiSeq sequencing. The principal coordinate analysis showed that changes of bacterial community structure due to eT and eTeCO2 were greater in soybean- than maize-grown soils. The eT increased the relative abundances of Gaiella and Bacillus in Actinobacteria and Firmicutes, but decreased those of Nocardioides and H16 in Actinobacteria and Proteobacteria, respectively. The magnitudes of responses of seven genera sensitive to eT varied between soybean- and maize-grown soils. The eTeCO2 decreased the relative abundance of Bacillus and increased those of Gaiella, Streptomyces and Mizugakiibacter. The abundances of Gaiella, Gemmatimonas, and Mizugakiibacter under eTeCO2 were higher in soybean- than maize-grown soils. The redundancy analysis showed that soil organic C, moisture, nitrate, microbial biomass N and Olsen-P significantly affected soil bacterial community composition. All these results indicate that long-term eT increased the abundance of bacterial community and shifted their composition compared to the ambient control. In addition, the bacterial community composition under eTeCO2 was more stable in maize- than soybean-grown soils. The study suggests that warming and crop species may interactively affect the stability of bacterial community linking to the sustainability of soil eco-function in future cropping systems.

Responses of soil total phosphorus to freeze and thaw cycles in a Mollisol watershed

Freeze-thaw cycles (FTCs) change soil physiochemical properties and biogeochemical processes and inevitably influence the spatial distribution of soil phosphorus (P). In this study, a field investigation was conducted on the Mollisol soil of the Guangrong watershed from 2016 to 2018 to clarify the effect of FTCs on the spatial distribution of total phosphorus (TP) at soil depths of 0–5, 5–10 and 10–20 cm. A laboratory incubation experiment was conducted to reveal the effect of FTCs on TP in soil profiles (0–30 cm) in Mollisols. The results showed that (1) the spatial autocorrelation (nugget to sill ratio) of TP decreased by 5.92% after the FTCs while the spatial variance (coefficient of variation) increased by 17.19%. The TP in 85% of the watershed area decreased after the FTCs, and the mean content of TP decreased by 9.53%. These changes were mainly influenced by land use types, topographical factors, soil properties and human activities. (2) TP increased in the intersection between farmland and forestland (0.01 g kg−1) after the FTCs, while TP decreased both in the areas near the hydrological channel (0.06 g kg−1) and at the outlet of the watershed (0.13 g kg−1). Compared with down-slope tillage, maize planting, south-facing slopes and low vegetation coverage areas, the cross-slope tillage, soybean planting, north-facing slopes and high vegetation coverage areas reduced TP losses by 56.1%, 19.5%, 115.8% and 141.9%, respectively, in the 0–20 cm soil layer. (3) The effects of FTCs on TP in soil profile were mainly influenced by the soil moisture content, FTC frequency and soil bulk density in the laboratory incubation experiment. TP variability showed a V-shaped trend as the number of FTCs increased, and decreased as the initial moisture content increased at 1.1 g cm−3 of soil bulk density. (4) An equation based on the TP and mean value of normalized difference snow index, normalized difference vegetation index, ferrous minerals index and normalized difference soil moisture index from the freezing period to the thawing period was built to roughly predict the TP after FTCs (R2 = 0.72). Generally, the TP tended to decrease during both the freezing stage and the thawing stage in the field. Areas with down-slope tillage, existing maize fields, south-facing positions, low vegetation coverage and high snow coverage should be focused on to reduce P losses during FTCs.

The effects of freeze-thaw cycles at different initial soil water contents on soil erodibility in Chinese Mollisol region

Freeze-thaw action is a common natural phenomenon in cold regions, which affects soil erodibility by changing soil structure and mechanical properties. However, quantifying the impacts of freeze-thaw cycles (FTCs) at different initial soil water contents (ISWCs) on soil erodibility is challenging due to the complex interactive soil mechanical responses. In this study, direct shear strength and soil disintegration tests were conducted to quantify soil erodibility indices. Seven FTCs (0, 1, 3, 5, 7, 10 and 13) and three ISWCs (16.5, 24.8 and 33.0%) were employed to investigate soil shear behavior and disintegration properties for two Mollisol soils. Results showed that repeated FTCs had cumulative impacts on soil mechanical properties, and the 10th FTCs might be the threshold number for influencing soil erodibility. The soil shear strengths decreased by 1.1–15.1% and 9.2–30.5% under four normal stresses at the first and 13th FTCs, respectively, for these two soils. Soil cohesion generally decreased with the increase of FTCs. Soil internal friction angle showed no trend with FTCs at the 16.5% and 24.8% water contents but a decreasing trend at the 33.0% water content. Soil disintegration rate increased with FTC and reached a maximum value at the 10th cycle but decreased with the increase of ISWC. Soil disintegration of the two Mollisols showed a sharp disintegration of about 60% in 133 s. This study provides a new understanding of the response of erodibility to freeze-thaw conditions for two Mollisols.

Dynamics of maize straw–derived nitrogen in soil aggregates as affected by fertilization

The addition of maize residue nitrogen (N) to the soil strongly influences soil N accumulations, but the specific contributions of maize residue N to soil aggregates based on long-term fertilization remain largely unknown. This study involved a 360-day laboratory incubation experiment to determine the dynamics of N derived from maize straw in Mollisol soil aggregates applying different long-term fertilization treatments. In 2015, three soil samples from different fertilizers treatments were collected from the upper layer of soil (0–20 cm) of the field at a long-term Mollisol (Luvic Phaeozem) experimental site established in 1980 in Gongzhuling, Jilin Province, China. The fertilizer treatments included no fertilizer (CK); a combination of nitrogen, phosphorous and potassium fertilizers (NPK); and NPK combined with manure (MNPK). Three treatments of soils were incubated for 360 days at 25 °C, with or without 15N-labeled maize straw and destructively collected on days 45, 90, 135, 180 and 360. Soil samplings were separated into two aggregate fractions (macroaggregates, ≥ 0.25 mm; microaggregates, NPK > CK after 360 days of incubation. The results revealed that the combined application of chemical fertilizer and organic manure had higher capacity to retain maize straw–derived N, and more of it was retained in macroaggregates in the beginning. The addition of straw residue accelerated the formation of macroaggregates in the soils with lower C/N ratios.

Wetland geomorphology and paleoecology near Akab Muclil, Rio Bravo floodplain of the Belize coastal plain

To understand wetland geomorphology and paleoecology, we collected a 2.6 m sediment core from a flooded swamp adjacent to the Maya archaeological site of Akab Muclil in the Maya Lowlands of northwestern Belize. The site of Akab Muclil has a known occupation that persisted from Early Maya Classic (1700–1350 BP) through the Terminal Maya Classic (1180–1050 BP) and into the Postclassic (1050–450 BP) and lies near a vast network of ancient Maya canal and field systems. We analyzed this core using a combination of paleoecological and geochemical techniques to determine the history of land use and natural change over time within this wetland. Accelerator Mass Spectrometry dating, pollen, charcoal analysis, micropaleontology, geochemical analysis, and magnetic susceptibility provide a suite of methods from which we interpret the geomorphic and ecological history of this wetland system. Four AMS dates from the length of the core provide us with an age model that runs from 1675 cal BP through the Maya Classic onward to the present. At the base of this system, soil composition and chemistry provide evidence that the system changed from a seasonally wet terrestrial soil to a perennially wet swamp, as the basal Mollisol soil lies buried by peats and calcareous sediments. This shift to a perennial wetland could be related to ancient Maya water management or a natural geomorphic change, though we suspect the former because of nearby ancient Maya large-scale geomorphic and hydrological manipulation in the form of intensive canalization and agriculture. Evidence of ancient Maya uses and impacts, including sedimentation, Zea mays pollen, and high charcoal counts occur from the lowest levels of the sequence through the Classic and into the Postclassic period. Above this level, the strata change to stable peats, laminated deposits of light gray/dark gray gypsum, authigenic carbonate, and layers of fibrist peat, with little evidence of human impact until recent increases in charcoal and phosphorous. This study, compared with other regional studies, indicates a later transition from terrestrial to wetland, later human impacts in the Postclassic, and a geomorphic impact record closely tied to the history of the adjacent site rather than broader land use trends.

Spring camelina N rate: balancing agronomics and environmental risk in United States Corn Belt

ABSTRACTCamelina (Camelina sativa (L.) Crantz) seed oil has desirable properties for producing advanced biofuels and as a healthy cooking oil. It has been grown for centuries, but basic recommendations for nitrogen (N) fertilizer requirements are still needed to support widespread industrial cultivation across North America. A replicated N-response plot-scale study was conducted on a northern Mollisol soil for two growing seasons to 1) determine seed and oil yield, seed oil content, and vegetative response; 2) determine indices of N use efficiency; and 3) measure post-harvest residual inorganic soil N as an index of environmental risk. Seed and oil yield response to N fertilization was described with a quadratic function, which predicted maximum seed yield (1450 kg ha−1) and oil yield (580 kg ha−1) at about 130 kg N ha−1. However, seed and oil yield did not differ significantly among N-rates above 34 kg N ha−1. Seed oil content averaged 400 g kg−1 among all N rates. Agronomic efficiency declined above 34 kg N ha−1, which coincided with an increase of post-harvest soil nitrate-N plus ammonium-N (residual N). Considering N use efficiency, simple cost analysis, and risk associated with residual N, a rate of 34 kg N ha−1 is recommended.

Root distribution patterns of reticulatus and inodorus melon (Cucumis melo L.) under subsurface deficit irrigation

Information on root growth patterns is crucial to understand cultivar adaptations to deficit irrigation, particularly for subsurface drip-irrigated crops, where root systems are more confined than furrow or sprinkler systems. A 2-year field experiment evaluated the effect of deficit irrigation (50% vs. 100% crop evapotranspiration, ETc) on root growth of melon (Cucumis melo L.) cultivars (Mission, Da Vinci, and Super Nectar) on a mollisol soil in southwestern Texas, USA. Root length intensity (La; mm cm−2), and root length density (RLD; cm cm−3) were measured at different growth stages using the minirhizotron and soil core methods, respectively. A higher La was recorded in 2012 than in 2011 (3.71 mm cm−2 vs. 0.50 mm cm−2), due to differences in temporal distribution of rainfall between years. Overall, deficit irrigation increased La as compared to 100% ETc, but significant effects occurred at depth (> 40 cm below the soil surface and > 25 cm below the subsurface drip tape). In 2012, moderate water deficit promoted deep root growth at the fruit setting stage but not in 2011, while root growth responses to cumulative deficit varied with cultivar. In 2012, deficit irrigation enhanced root growth in cv. Mission (cantaloupe; reticulatus) throughout the season, it was maintained in cv. Da Vinci (Tuscan; reticulatus), while it decreased in cv. Super Nectar (honeydew; inodorus) at the final harvest stage. Thus, the reticulatus melon cvs. Mission and Da Vinci can better adjust to moderate water-deficit conditions as compared to the inodorus melon cv. Super Nectar. Minirhizotron data provided useful information on root growth dynamics in deeper soil layers in a non-destructive way, which makes it a valuable tool in screening cultivars for water deficit adaptation.

Microbial responses to inorganic nutrient amendment overridden by warming: Consequences on soil carbon stability.

Eutrophication and climate warming, induced by anthropogenic activities, are simultaneously occurring worldwide and jointly affecting soil carbon stability. Therefore, it is of great interest to examine whether and how they interactively affect soil microbial community, a major soil carbon driver. Here, we showed that climate warming, simulated by southward transferring Mollisol soil in agricultural ecosystems from the cold temperate climate zone (N) to warm temperate climate (C) and subtropical climate zone (S), decreased soil organic matter (SOM) by 6%-12%. In contrast, amendment with nitrogen, phosphorus and potassium enhanced plant biomass by 97% and SOM by 6% at the N site, thus stimulating copiotrophic taxa but reducing oligotrophic taxa in relative abundance. However, microbial responses to nutrient amendment were overridden by soil transfer in that nutrient amendment had little effect at the C site but increased recalcitrant carbon-degrading fungal Agaricomycetes and Microbotryomycetes taxa derived from Basidiomycota by 4-17 folds and recalcitrant carbon-degrading genes by 23%-40% at the S site, implying a possible priming effect. Consequently, SOM at the S site was not increased by nutrient amendment despite increased plant biomass by 108%. Collectively, we demonstrate that soil transfer to warmer regions overrides microbial responses to nutrient amendment and weakens soil carbon sequestration.

Elevated CO2 increases the abundance but simplifies networks of soybean rhizosphere fungal community in Mollisol soils

Elevated atmosphere CO2 (eCO2) levels lead to changes in the quantity and composition of rhizodeposition of soybeans. Previously, a majority of studies have focused on the bacterial community response to the eCO2 in the rhizosphere of soybean with little information regarding the quantitative and compositional changes in the fungal community available. To provide insight into the fungal community response, next generation sequencing of the internal transcribed spacer (ITS) region was conducted for in-depth analysis of changes in fungal abundance and diversity in response to eCO2. Four soybean cultivars (i.e. Xiaohuangjin, Suinong 8, Suinong 14 and Heinong 45) were grown for 65 days under ambient CO2 (aCO2) (390 ppm) and eCO2 (550 ppm) in Mollisol soils. Elevated CO2 significantly increased ITS copy numbers in the rhizosphere of the soybean cultivars except Xiaohuangjin and Suinong 14. Principal coordinate analysis (PCoA) revealed that eCO2, rather than soybean cultivars, altered the composition of soil fungal communities. Network analysis indicated that eCO2 simplified the network structure by changing topological roles of operational taxonomic units (OTUs) and key fungal members, which were likely regulated by concentrations of NH4+-N, NO3−-N and available K and microbial biomass C under eCO2.

Root Ideotype Influences Nitrogen Transport and Assimilation in Maize.

Maize (Zea mays, L.) yield is strongly influenced by external nitrogen inputs and their availability in the soil solution. Overuse of nitrogen-fertilizers can have detrimental ecological consequences through increased nitrogen pollution of water and the release of the potent greenhouse gas, nitrous oxide. To improve yield and overall nitrogen use efficiency (NUE), a deeper understanding of nitrogen uptake and utilization is required. This study examines the performance of two contrasting maize inbred lines, B73 and F44. F44 was selected in Florida on predominantly sandy acidic soils subject to nitrate leaching while B73 was selected in Iowa on rich mollisol soils. Transcriptional, enzymatic and nitrogen transport analytical tools were used to identify differences in their N absorption and utilization capabilities. Our results show that B73 and F44 differ significantly in their genetic, enzymatic, and biochemical root nitrogen transport and assimilatory pathways. The phenotypes show a strong genetic relationship linked to nitrogen form, where B73 showed a greater capacity for ammonium transport and assimilation whereas F44 preferred nitrate. The contrasting phenotypes are typified by differences in root system architecture (RSA) developed in the presence of both nitrate and ammonium. F44 crown roots were longer, had a higher surface area and volume with a greater lateral root number and density than B73. In contrast, B73 roots (primary, seminal, and crown) were more abundant but lacked the defining features of the F44 crown roots. An F1 hybrid between B73 and F44 mirrored the B73 nitrogen specificity and root architecture phenotypes, indicating complete dominance of the B73 inbred. This study highlights the important link between RSA and nitrogen management and why both variables need to be tested together when defining NUE improvements in any selection program.

Occurrence and removal of pharmaceutical compounds and steroids at four wastewater treatment plants in Hawai'i and their environmental fate

The occurrence of pharmaceutical and steroid compounds in groundwater due to wastewater reuse has been reported and is of concern in tropical islands which primarily rely on groundwater. The objective of this study was to investigate the occurrence and removal of 43 pharmaceutical and steroid compounds detected in wastewater at four different wastewater treatment plants (WWTPs) in Hawai‘i and to understand their environmental behavior through tropical soils as the treated effluents are used in landscapes for irrigation. Eight soil sampling locations, collected at three different depths, representing the most common soil types in Hawai‘i and four WWTPs located across the major Hawaiian Islands were used. Disturbed soil samples were used to conduct the soil sorption and degradation studies and to estimate the leaching risk associated to the identified compounds. Quantification of selected compounds was conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS).Among the investigated compounds, only ten were detected in the treated effluents at concentrations ranging from 0.004 to 0.900 μg L−1. Caffeine (64 μg L−1) and ibuprofen (96.5 μg L−1) showed the highest concentration in raw samples, while diphenhydramine (0.9 μg L−1) showed the highest concentration in treated effluent samples. Sulfamethoxazole showed the lowest removal (0–75%). Several pharmaceuticals showed consistently higher sorption capacity and longer persistency compared with steroids regardless of soil types and depths. Poamoho (Oxisol soil) and Waimānalo (Mollisol soil) showed the highest sorption capacity, while Waimea (Entisol soil) showed the lowest sorption capacity. Soil physico-chemical properties (i.e., clay content, level of organic carbon, and presence of metal oxide) and soil depth highly impacted the sorption behavior of the selected pharmaceutical compounds. In particular, the sorption capacity decreased with soil depth due to the higher level of organic carbon present in the first 30 cm compared with the deeper depths (60–90 cm).