Molecular Imprinting Research Articles

A dual-capture-system polymer based on imprinted cavities and post-imprinting modification sites with significantly improved affinity and specificity for sialic acid and sialylated glycoprotein

The abnormal expression of N-acetylneuraminic acid (SA) and sialylated glycoproteins in biological fluids are closely associated with various diseases including cancer. However, the low content of SA and the strong interference of complex matrix greatly influence the effective capture of SA in biosamples prior to analysis. Herein, a dual-capture-system strategy based on molecular imprinting and post-imprinting modification (PIM) was proposed to precisely capture SA with improved binding affinity and specificity. After imprinting with SA as template, dynamic imine bonds are introduced by post-imprinting modification, enabling sufficiently high specificity to capture SA through imprinting cavities and the dynamic imine bonds hydrolysis reaction simultaneously. The prepared magnetic PIM polymers (Mag-MIPs-PIM) exhibited significantly high specificity both for SA (IF = 4.24) and sialylated glycoprotein (IFTRF = 3.50). In addition, the feasibility of Mag-MIPs-PIM for practical application was demonstrated by association with HPLC for the determination of SA in human serum, and an LOD of 0.01 × 10−2 g L−1 was obtained. The proposed strategy based on molecular imprinting and PIM provides a new inspiration for the improvement of selectivity of the molecularly imprinted polymers.

Magnetic Molecularly Imprinted Polymer Combined with Solid-Phase Extraction for Purification of Schisandra chinensis Lignans

Molecularly imprinted polymers (MIPs) can specifically recognize template molecules in solution with imprinted cavities. Due to their capacity for scalable production, they can be used to isolate target products from natural products for industrial production in the fields of pharmaceuticals and food. In this study, magnetic single-template molecularly imprinted polymers (St-MIPs) instead of magnetic multi-template molecularly imprinted polymers (Mt-MIPs) were prepared by surface imprinting using Schizandrol A as a template molecule and deep eutectic solvent (DES) as a functional monomer, combined with solid-phase extraction (SPE) for the adsorption and separation of Schizandrol A, Schisantherin A, Schizandrin A, and Schizandrin B from Schisandra chinensis (Turcz.) Baill. (S. chinensis) fruits extracts. The synthesized MIPs were characterized by FT-IR, TEM, SEM, TG, XRD and VSM, and their adsorption properties were also evaluated. MIPs can specifically recognize the template molecules with high reusability. The purity of the total S. chinensis lignans after SPE was 74.05%, among which that of Schizandrol A, Schisantherin A, Schizandrin A, and Schizandrin B was 33.38%, 8.69%, 16.33% and 15.67%, respectively. Moreover, the one-step synthesis of carrier was easy to operate. And St-MIPs reduced the production cost compared with Mt-MIPs. This study provides a new idea for natural product separation by molecular imprinting technology (MIT).

Electrochemical Determination of B-Type Natriuretic Peptide with an Epitope-Imprinted Polymer-Based Sensor

B-type natriuretic peptides (BNP) are produced and secreted by the myocardium to reduce blood pressure and cardiac load. They cause vasodilation, natriuresis, growth suppression, and inhibition of the sympathetic nervous system and the renin–angiotensin–aldosterone system. The measurement of plasma BNP levels provides clinically useful information concerning the diagnosis and management of left ventricular dysfunction and heart failure, complementing other diagnostic testing procedures. In this work, three epitopes from the N-terminal (BNPnt), C-terminal (BNPct), and the cystine-bridged cyclic peptides (BNPr) of B-type natriuretic peptides were synthesized as templates for molecular imprinting. These peptides were doped into aniline (AN) and m-aminobenzenesulfonic acid (MSAN) for electropolymerization, thus forming epitope-imprinted poly(AN-co-MSAN) conductive films (EIPs). The monomer ratio was optimized using the electrochemical signals during polymerization. The optimized films were then characterized using a scanning electron microscope (SEM), atomic force microscope (AFM), and AC impedance. The electrochemical response of the films to the target peptides and to BNP was then measured. The sensing range of the EIPs-coated electrodes was from 0.001 to 1000 pg/mL for BNP. Finally, the BNP concentration in diluted serum samples was measured with the BNPrIP-coated electrode, giving 3.15 ± 0.07 pg/mL. By spiking the sample with known BNP concentrations, the accuracy was determined to be better than ±5%.

Flexible Au@Ag/PDMS SERS imprinted membrane combined with molecular imprinting technology for selective detection of MC-LR

In this study, a core–shell structured bimetallic nano-cube, Au@Ag NCs, was prepared by seed-mediated growth procedure. The array structure of Au@Ag NCs was achieved at the interface through the autonomous assembly technique at the three-phase boundary. Employing polydimethylsiloxane (PDMS) as a flexible carrier, the array structure was effortlessly transferred to the PDMS membrane, bypassing the need for rigid substrates through a simple “pasting” method. This yielded a highly flexible and transparent SERS substrate with an array structure (Au@Ag NCs/PDMS membrane, AAP). In order to promote the selective detection property to the practical samples, molecularly imprinted polymers (MIPs) were coated on the surface of membrane to prepare the imprinted membrane (Au@Ag NCs/PDMS-MIMs, AAP-MIMs). It was demonstrated from the results that the AAP-MIMs exhibited high SERS sensitivity, stability, and uniformity. Furthermore, the flexible substrate possessed commendable mechanical strength, and facilitated the detection of analytes on irregular surfaces. In summary, this substrate held promising potential for practical on-site detection and analysis of specific target substances.

Boronate Affinity Molecular Imprinting Based Sugar and Ovalbumin Fiber Optic SPR Sensor

Boronate Affinity Molecular Imprinting Based Sugar and Ovalbumin Fiber Optic SPR Sensor

Analysis of Trace Enrofloxacin in Environmental Waters by a Surface Molecular Imprinting Technique.

Surface-imprinted polymers (ZIF-67@MIPs) supported by ZIF-67 were prepared by precipitation polymerization using enrofloxacin (ENR) as the template molecule, methacrylic acid as the functional monomer, and ethylene glycol dimethacrylate as the cross-linker. ZIF-67@MIPs were characterized by Fourier transform infrared spectrometry, X-ray diffraction, scanning electron microscopy, and particle size distribution. The adsorption performance of the polymer was studied. The adsorption equilibrium was reached within 30 min. The maximum adsorption was 9.02 μg·mg-1. The imprinting factor was 2.58. The polymer was then used as a sorbent of a solid-phase extraction column for the separation and purification of ENR in real water samples. The extraction conditions were optimized. The method was established by high-performance liquid chromatography, and the linearity was verified by UPLC-MSMS. The correlation coefficient and limits of detection and quantification were 0.9999, 0.23 ng·mL-1, and 0.76 ng·mL-1, respectively. The recoveries were in the range of 83.79-100.68%; the relative standard deviation was 4.46-7.35%. The above data indicated that the method could be used for the separation and enrichment of ENR in real samples.

Developing a Molecular Imprinting Sensor Based on Double-Layer Coating Structure for the Rapid and Sensitive Detection of Chlortetracycline in Milk

Developing a Molecular Imprinting Sensor Based on Double-Layer Coating Structure for the Rapid and Sensitive Detection of Chlortetracycline in Milk

Molecular imprinting sensor based on zeolitic imidazolate framework derived Co, N-doped carbon loaded on reduced graphene oxide toward the determination of dopamine.

Anovel voltammetric sensor designed for dopamine(DA) detectionis presented utilizing a combination of zeolitic imidazolate framework (ZIF-67) derived cobalt and nitrogen-doped carbon on reduced graphene oxide (Co-N-C/rGO). ZIF-67 cubic crystals were synthesized in situ and deposited onto the graphene oxide (GO) surface through room-temperature reactions. High-temperature calcination resulted in partially collapsed cubic and spherical carbon, while simultaneously reducing GO to rGO. A molecular imprinting resorcinol polymer (MIP) membrane was also in situ applied to the Co-N-C/rGO/glassy carbon electrode (GCE) via electropolymerization. Analyses using cyclic voltammetry, electrochemical impedance, and pulse voltammetry reveal that the modified MIP/Co-N-C/rGO/GCE electrodes show improved electroconductivity and notable electrochemical reactivity towards dopamine. After optimizing detection parameters, the sensor demonstrates a wide linear detection range of 0.01-0.5 and 0.5-100μmol/L, with a limit of detection (LOD) of 3.33nmol/L (S/N = 3). Additionally, the sensor displays strong robustness, including excellent selectivity, significant resistance to interference, and long-term stability. It also shows satisfactory recovery in detecting spiked real samples.

Electrical properties of acetone imprinted hematite nanomaterials doped with Pd & Ag for gas sensing and simulation of their wireless devices

This article presents a novel technique for wireless hydrothermally improved gas sensor devices enhanced by molecular imprinting technique (MIT) for hematite nanomaterials with the existence of acetone using different ratios (10 %, 15 %, and 25 %) as well as doping with palladium/ silver to detect the imprinted gas. α-Fe2O3 are characterized utilizing field emission scanning electron microscope (FESEM), photoluminescence, Fourier transforms infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray photo-spectroscopy (XPS), and High-Resolution Transmission Electron Microscopy (HRTEM), Thermogravimetric Analysis (TGA) and utilizes Brunauer-Emmett-Teller (BET) to ascertain their shape, optical properties, and values for surface area and thermal characteristics, respectively. Depending on the preparation conditions, non-regular cubes and rods have typical particle sizes ranging from 25 to 120 nm, acetone imprinted and doped with palladium sample (Ap) offers smaller particles than acetone imprinted sample (A15) making adsorption easier. Where XRD showed all diffraction peaks for α-Fe2O3 as well as XPS authorized the oxidation state, also FTIR showed the characteristic peaks of the stretching mode Fe-O which indicates the formation of α-Fe2O3. Lower defects for (Ap) as it has the highest intensity of 176.15 a.u. in the photoluminescent spectrum, for TGA analysis results explain that pure sample has more thermal stability and lower weight loss. Six hematite films are fabricated using the spin coating technique where acetone molecules are presented during the synthesis process of the nanomaterials to imprint their shapes on the surface of hematite. By measuring the response of the gas sensors and their electrical properties, the I-V curve for Ap showed a rectifying behavior and its shunt resistance (Rsh) is higher than series resistance (Rs) which ensures a high response of (115 %). To develop a wireless gas sensor COMSOL Multiphysics software 5.3(a) software is used for the simulation of three devices model by depositing a layer by specified dimensions of hematites (A15, As, and Ap) with their different electrical conductivities on the surface of rectangular patch antenna by showing geometry, the microstrip patch antenna bandwidth, resonant frequency, scattering parameters, and radiation patterns in the E-plane and 3-D far-field pattern and directivity is calculated also, Sp gives improved reflection coefficient, gain quality factor and directivity due to its high electrical conductivity.

Fabrication of ratiometric molecular imprinting electrochemiluminescence sensor based on polyethyleneimine hydrogel loading layered bimetallic hydroxide and MIL-125@Ru(bpy)32+ for sensitive tetracycline detection

In this work, layered Co-Ni bimetallic hydroxide was synthesized and combined with polyethyleneimine hydrogel (PEI@LDH) as substrate material, then the metal-organic framework MIL-125 loaded Ru(bpy)32+ (MIL@Ru) was added as single luminophore, followed by electrochemical deposition of tetracycline (TC) imprinted copolymer of hydroquinone and o-phenylenediamine to construct an electrochemiluminescent (ECL) sensor for TC detection. The stable and sensitive luminophore MIL@Ru associating with co-reactant K2S2O8 in the solution could cause a cathode ECL signal sensing TC, it could also associate with the immobilized co-reactant PEI hydrogel to produce a stable anode ECL signal acting as a reference. The LDH had catalysis and could efficiently amplify both anode and cathode ECL signals. Therefore, the constructed single luminophore based ratiometric ECL sensor displayed high selectivity, sensitivity and reproducibility. Under the selected testing conditions, the linear response range was 0.01 μmol L−1 to 1 mmol L−1 and the detection limit reached 8 nmol L−1. Its good practicability was validated by determining TC in different actual samples. Hence, the ratiometric MIP-ECL sensor was quite promising.

A molecularly imprinted electrochemiluminescence sensor based on mimic enzyme ZIF-90 and MnO2/g-C3N4 magnetic particles for detection of methidathion.

Methidathion (MTDT), a common organophosphorus pesticide with high insecticidal activity, is widely used for pest control. However, the misuse of MTDT leads to widespread residues and endangers human health. Therefore, it is crucial to develop a simple and highly sensitive method for the detection of MTDT residues. Herein, ZIF-90/MnO2/g-C3N4/Fe3O4 composite particles were synthesized: The MnO2 nanosheets could absorb the energy of the excited g-C3N4 to quench the ECL of g-C3N4 while ZIF-90 acted as a mimetic enzyme to catalyze the formation of thiocholine from MTDT. The thiocholine caused the reduction of MnO2 to Mn2+, restoring the ECL signal of g-C3N4. Combined with molecular imprinting technique, an electrochemiluminescence sensor was constructed for the determination of MTDT. The determination range was 1.00 × 10-9 ~ 7.00 × 10-7g/L, and the detection limit was 6.58 × 10-10g/L. Structurally similar organophosphorus pesticides showed no cross-reactivity. The method has high sensitivity and specificity, and has been successfully applied to the determination of MTDT residue in fruits with recoveries in the range 93.75% ~ 102.37%.

Molecularly imprinted polymer for the selective removal of direct violet 51 from wastewater: synthesis, characterization, and environmental applications

Abstract Molecularly imprinted polymers (MIPs) are a diverse class of materials designed for selective molecular recognition. These polymers are synthesized with particular binding sites that are suited to a target molecule or a collection of structurally similar molecules through the use of a process called molecular imprinting. MIPs were synthesized in this work to specifically remove direct violet 51 from occupational leachates and aqueous solutions. Methacrylic acid functioned as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, 2,2-azobisisobutyronitrile (AIBN) as the initiator, and alcohol as a porogenic solvent. To improve the dye removal effectiveness, a number of factors were optimized, including time, pH, analyte concentrations, and MIP/NIP dosages. The findings showed that MIPs had a much greater capacity for direct violet 51 adsorption than nonimprinted polymers (NIPs), with MIP adsorption capacity reaching 42.553 mg g−1 and NIP adsorption capacity reaching 7 mg g−1. The pseudo 2nd-order model described the adsorption kinetics, and the rate constant (K 2) for MIPs was found to be 0.00251 mg g−1 min. Furthermore, a high rebinding efficiency of 94 % was observed when the selectivity of MIPs for direct violet 51 was assessed against structurally similar templates.

Amplified Sensitivity in SERS Detection of L1CAM With Silver Plasmonic Mesoporous Silica Capsules on an Imprinted Films

AbstractThis study presents a novel approach for dual detection, leveraging a combination of a Raman reporter‐bearing nanomaterial and molecular imprinting polymers (MIP). A core‐shell Au‐Ag nanoparticles (Au‐Ag NPs) encapsulated in mesoporous silica nanocapsules (Au‐Ag NCs) and a new MIP‐based material targeting L1CAM are used. The MIP prepared via surface imprinting on a carbon screen‐printed electrode (C‐SPE) used thionine (TH) as a monomer. The plasmonic Au‐AgNCs are further functionalized with the Raman reporter 4‐mercaptobenzoic acid (MBA) and anti‐L1CAM for selective detection by surface‐enhanced Raman scattering (SERS) spectroscopy. The biosensor's analytical performance is evaluated using both SERS and electrochemical impedance spectroscopy (EIS). EIS analysis reveals a linear response within the concentration range of 0.1 to 100 ng mL−1 in buffer and serum samples. SERS demonstrates a sensitivity ten times higher than EIS. Selectivity study demonstrates the biosensor's excellent specificity toward L1CAM, with minimal interference from other compounds such as creatinine, glucose, and carbohydrate antigen 19‐9 (CA 19‐9). The Raman signal from the reporter molecule correlates with increasing L1CAM concentrations, reinforcing the analytical findings obtained through electrochemical analysis. Thus, the combination of dual detection and recognition capabilities presents promising potential for detecting diverse biomarkers, especially in critical scenarios where reducing false‐positive or false‐negative errors is crucial.

Hydrogel Grating Sensors with Boron Affinity and Molecular Imprinting Effects for Rapid and Sensitive Detection of Tumor Marker Sialic Acid.

Rapid and sensitive detection of the concentration of sialic acid (SA) in serum is crucial for early tumor screening and prognostic assessment; however, it still remains challenging. Here, we propose a novel kind of hydrogel grating sensor with boron affinity and molecular imprinting effects (B-MIP) for the rapid and sensitive detection of SA concentration in serum. The hydrogel gratings feature uniform surface relief microstructures and incorporate highly specific recognition binding sites into SA molecules provided by boron affinity and molecular imprinting. The periodic nanoridges of hydrogel gratings increase the specific surface area contacting the environmental solution; therefore, fast detection can be achieved within 2 min. Upon recognition of SA molecules, the height of hydrogel gratings changes at the nanoscale, causing a change in the diffraction efficiency of the hydrogel gratings. The B-MIP hydrogel grating sensors have highly specific binding sites to SA molecules distributed throughout the whole hydrogel and can preferentially and selectively recognize and respond to the SA molecules even in the presence of interference substances glucose and fructose with high concentrations. The B-MIP hydrogel grating sensors are effectively applicable for the rapid and sensitive detection of SA concentrations in real serum samples with satisfactory accuracy and precision. Our approach provides an excellent strategy to address the current challenges in SA detection and provides new insights into the detection of tumor markers in serum, thereby opening up new ways to accurately detect complex biological samples in analytical science.

Calixarene incorporated molecular imprinting on covalent organic framework for supramolecular recognition and specific extraction of citrinin

Specific recognition and selective extraction of mycotoxin in environmental and food matrixes is significant to guarantee public health. Covalent organic frameworks (COFs) are promising adsorbents with tailorable functionality, but their low binding affinity and poor selectivity hamper their wide application for selective extraction of trace mycotoxin from complex matrix. Herein, we report calixarene incorporated molecular imprinting on COF to prepare molecularly imprinted calix[4]arene-containing COF (MICOF-CX4) for supramolecular recognition and specific adsorption of citrinin. Calixarene with host-guest chemistry was used as a functional monomer, while amine units with different topologies and function groups were selected to regulate MICOF-CX4 to match with citrinin. The complementary shape and supramolecular interactions of MICOF-CX4 gave highly selective recognition for citrinin. Moreover, MICOF-CX4 with vast accessible surface and plentiful imprinting sites exhibited faster adsorption kinetics and 4-fold higher adsorption capacity for citrinin adsorption than no-imprinted COF-CX4. Combination of MICOF-CX4 based solid-phase extraction with high-performance liquid chromatography-mass spectrometry allowed interference free determination of trace citrinin in real samples with a low detection limit of 0.03 ng mL−1, good precision of 4.5 % and quantitative recovery of 88.2 %−101.4 %. The cooperative functions of calixarene and molecular imprinting make COF promising adsorbent for specific adsorption of trace targets in complex matrixes.

A novel molecularly imprinted solid phase based on SiO2-Ag2O double-nucleation doped with graphene oxide for extraction and determination of flavonoids from Acanthaceae ebracteatus via HPLC-MS/MS

(Objectives) A novel method was developed for extracting and determining flavonoids from marine mangrove medicinal plants. This approach utilizes surface molecular imprinting to increase selectivity. Graphene oxide doped with a SiO2–Ag2O dual core structure served as a scaffold for synthesizing multilayer surface-imprinted polymers. The surface of the SiO2-Ag2O@GO composite was modified through self-polymerization. (Methods) Acacetin molecularly imprinted polymers (AMIPs) were created via surface-initiated precipitation polymerization with acacetin as the template. These AMIPs were synthesized and characterized via ultraviolet (UV) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), and transmission electron microscopy (TEM). Recycling experiments demonstrated the stability of the AMIPs. Analysis of the adsorption mechanism via the Langmuir and Freundlich models indicated that the Langmuir model was more suitable for predicting AMIPs. (Results) Scatchard plots showing two different binding sites of AMIPs for acacetin adsorption at various concentrations. The class selectivity of AMIPs for flavonoids was found in Acanthaceae ebracteatus. Compared with other analogs, competitive binding experiments revealed that the polymer had greater selectivity for acacetin. After AMIP solid-phase extraction (AMISPE), the linearity, LOD, LOQ, intraday and interday variabilities, recovery, and repeatability of AMIPs combined with HPLC–MS/MS were validated, confirming the method’s selectivity, precision, accuracy, and reproducibility. The contents of acacetin, apigenin, kaempferol, luteolin, quercetin, and genistein in Acanthaceae ebracteatus were determined. (Conclusions) These results indicate that AMISPE coupled with HPLC–MS/MS is a new, simple, and practical method for extracting, purifying, and enriching flavonoids from complex samples, such as those of herbs and other natural marine plants.

Removal of As (V) from Water with Cryogels Prepared By Molecular Imprinting Technique

The aim of this study is to selectively remove As(V) ions, the most common type of arsenic in drinking water and especially surface water. For this purpose, a super macroporous polymeric cryogel column was prepared using the molecular imprinting technique. MAC was chosen as the functional monomer due to the high affinity of arsenic to sulfhydryl (-SH) functional groups. MAC monomer was synthesized from the amino acid cysteine. Physicochemical properties of HEMA-based synthesized poly(HEMA-MAC) cryogel were determined by SEM FTIR surface area and swelling. Adsorption studies from water were carried out in a continuous system. Different parameters such as pH, flow rate, temperature, ionic strength and time were studied to determine the optimum conditions for the removal of As(V) ion from water. The maximum As(V) removal of poly(HEMA-MAC) cryogel was 189.4µg/g polymer at pH: 5.0 and 15ppm concentration. In selectivity studies conducted in the presence of PO43-, SO42- and NO3- ions. According to the relative k values obtained from the selectivity experiments, As IIP cryogel shows 1.52 times more selectivity for As(V) ion than PO43- ion, 2.61 times more selectivity for SO42- ion and 1.53 times more selectivity for NO3- ion than NIP cryogel. From the theoretical calculations, it was found that the As (V) adsorption was fit with the Langmuir isotherm and the adsorption process obeyed pseudo-second order kinetics.

Direct Synthesis of Artificial Esterase through Molecular Imprinting Using a Substrate-Mimicking Acylthiourea Template.

Most reported artificial esterases hydrolyze only activated esters. We here report a one-pot synthesis of artificial esterases via molecular imprinting. An acylthiourea template hydrogen bonds with 4-vinylbenzoic acid and coordinates to a polymerizable zinc complex inside a cross-linkable surfactant micelle. Double cross-linking of the micelle yields a polymeric nanoparticle catalyst that mimics a metalloenzyme to activate a water molecule for nucleophilic attack on the bound ester. The catalyst hydrolyzes both activated and unactivated esters under mild conditions with selectivity.

Synthesis, pharmacological evaluation, and modeling of novel quaternary ammonium salts derived from β-carboline containing an imidazole moiety as angiogenesis inhibitors

In this study, a series of novel β-carboline condensed imidazolium derivatives (7a-7y) were designed and synthesized by incorporating imidazolium salt structures into β-carboline. The cytotoxicity of compounds 7a-7y was evaluated in various cancer cell lines, including lung cancer (A549), gastric cancer (BGC-823), mouse colon cancer (CT-26), liver cancer (Bel-7402), and breast cancer (MCF-7), using the MTT assay. Most compounds exhibited significant activity against one or more of the cancer cell lines. Notably, compounds 7 g, 7o, 7r, 7 s, 7u, 7v, 7x, and 7w showed the highest cytotoxic activity (IC50 < 2 μM) in the tested tumor cell lines. Compound 7x demonstrated cytotoxic activities of 1.3 ± 0.3 μM (for BGC-823), 2.4 ± 0.4 μM (against A549), 7.8 ± 0.9 μM (for Bel-7402), and 9.8 ± 1.4 μM (against CT-26). The chick chorioallantoic membrane assay revealed significant anti-angiogenic potential of compound 7x. Molecular imprinting studies suggested the anti-angiogenic effect of compound 7x might be attributed to inhibition of VEGFR2 kinase. Molecular docking and molecular dynamics further indicate that its activity may be primarily associated with the potential inhibition of VEGFR2. Our research outcomes have provided valuable lead compounds for the development of novel antitumor drugs and have offered beneficial insights for subsequent drug design and optimization.

Pharmacokinetics of pulmonary indacaterol in rat lung using molecular imprinting solid-phase extraction coupled with RP-UPLC

Indacaterol, a β2 agonist prescribed for long-term management of patients with chronic obstructive pulmonary disease and asthma. In this study the first MISPE cartridges was developed using indacaterol as a template for its selective extraction from rat lung tissues, enabling precise pharmacokinetic evaluation at the drug’s site of action. A molecular imprinting polymer was synthesized using indacaterol as a template, methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a cross-linker with a molar ratio (1: 4: 20). The polymer was characterized by a high binding capacity of 9840 ± 0.86 and high selectivity with an imprinting factor of 4.53 ± 0.12. The synthesized polymer was utilized as a sorbent in solid-phase extraction to purify and extract indacaterol from lung tissue matrix. The optimum molecularly imprinted solid-phase extraction (MISPE) conditions were 20.0 mg of molecular imprinting polymer and non-imprinting polymer, acetonitrile as the loading solvent, acetonitrile: water (20: 80; by volume) as the washing solvent, and methanol: acetic acid (90: 10; by volume) as the eluting solvent. A pharmacokinetic study was performed for indacaterol in rat lungs using the synthesized and optimized MISPE cartridge as a tool for sample purification. These parameters were determined in the lung tissues of rats emphasizing the local exposure of indacaterol to its target organ. The Cmax and Tmax were 51.020 ± 2.810 µg mL− 1 and 0.083 ± 0.001 h, respectively. The AUC 0−24 and AUC0 − inf were 175.920 ± 1.053 and 542.000 ± 5.245 µg h mL− 1, respectively. The elimination rate constant was 0.014 ± 0.00012 h− 1 and the half-life time was 48.510 ± 0.012 h. This study successfully developed and optimized MISPE cartridges using indacaterol as a template, enabling precise pharmacokinetic evaluation in rat lung tissues. The cartridges demonstrated high binding capacity and selectivity, providing crucial insights into the local exposure of indacaterol at its site of action.