The positive column of the DC glow discharge sustained in CO2 laser mixture (containing CO2, N2 and He at the ratio of 6:24:70) has been studied in pressure range 130÷1350 Pa and for discharge currents in range 10÷50 mA in two discharge tubes of the same shape, made from two different materials. Our main goal was the study of spectra by means of optical emission spectroscopy. We have focused on the investigation of intensities of spectral bands of the second positive system of molecular nitrogen corresponding to the transitions C3Π→B3Π and Angstrom band of CO molecules (transitions B1Σ→ A1Π) in reference to important role of this molecules in the discharge. It has been found that intensity of nitrogen bands decreases with increasing pressure and with decreasing current. It was possible to estimate vibrational temperature Tvib from measured intensities of above — mentioned bands of molecular nitrogen. It was observed that Tvib increases with increasing discharge current and slightly decreases with increasing pressure.