Event Abstract Back to Event Modulation of glucose uptake and insulin sensitivity in insulin resistant in vitro Alzheimer’s Disease model by vitamin D and E Nafissa Nadia Ibrahim1*, Huzwah Khaza'Ai1, Amirah Salwani Zaulkaffli1 and Norshariza Nordin1 1 Department of Biomedical Sciences, Faculty of Medicine and Health sciences, University of Putra Malaysia, Malaysia Background Currently, there is a widening recognition that impaired insulin signaling which causes a brain-specific form of diabetes contributes to the pathogenesis of Alzheimer’s Disease (AD). Previous study has reported that high concentration of insulin can alter expression of insulin signal transduction cascade such as insulin receptor (IR), glucose transporter (GLUT4), phosphatidylinositol 3-kinase (PI3K) and glycogen synthase kinase 3 (GSK3) which lead to inhibition of glucose uptake into the cell. Progressive derangements of brain glucose utilization due to insulin insensitivity cause the impairment of glucose homeostasis, hence increasing the oxidative stress which leads to the pathogenesis of AD. Vitamin D has been recognized to have a direct beneficial effect on insulin action by stimulating the expression of insulin receptors. Meanwhile, vitamin E possesses a neuroprotective effect by scavenging oxidative stress effectively. This study aims to elucidate the effect of vitamin D and E in modulating insulin signaling and AD markers in insulin resistant in vitro AD model. Methods SK-N-SH (neuroblastoma) was induced with high insulin prior to assess the mRNA expression of insulin signaling markers (IR, GLUT4 and PI3K), AD markers (TAU and GSK3β) and glucose uptake level. Treatment of vitamin D, E and a combination treatment were introduced for 24 hours upon completion of insulin induction. Results Improvement of insulin signaling pathway was observed upon treatment of vitamin D alone by significantly increase the level of IR, GLUT4 and PI3K, and glucose uptake level. Reduced expression of both AD markers was observed upon treatment with vitamin D. However, vitamin E and a combination treatment of both vitamins showed insignificant changes in insulin signaling and AD markers. Conclusion Vitamin D and E may demonstrate as an agent that slows the progression of Alzheimer Disease caused by insulin resistance in the brain. Keywords: Insulin Resistance, Alzheimer’s disease, Vitamin E, Vitamin D, neurodegenerative Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Metabolic diseases Citation: Ibrahim N, Khaza'Ai H, Zaulkaffli A and Nordin N (2019). Modulation of glucose uptake and insulin sensitivity in insulin resistant in vitro Alzheimer’s Disease model by vitamin D and E. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00068 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2018; Published Online: 17 Jan 2019. * Correspondence: Miss. Nafissa Nadia Ibrahim, Department of Biomedical Sciences, Faculty of Medicine and Health sciences, University of Putra Malaysia, Kuala Lumpur, Malaysia, nafissanadia@yahoo.com.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nafissa Nadia Ibrahim Huzwah Khaza'Ai Amirah Salwani Zaulkaffli Norshariza Nordin Google Nafissa Nadia Ibrahim Huzwah Khaza'Ai Amirah Salwani Zaulkaffli Norshariza Nordin Google Scholar Nafissa Nadia Ibrahim Huzwah Khaza'Ai Amirah Salwani Zaulkaffli Norshariza Nordin PubMed Nafissa Nadia Ibrahim Huzwah Khaza'Ai Amirah Salwani Zaulkaffli Norshariza Nordin Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.