Modified Atmosphere Packaging Research Articles

Effects of different packaging method on oxidation stability and tenderness of chicken breast injected with different types of kiwifruit during storage

SummaryThis study aimed to investigate the antioxidant and protease activities of red kiwifruit (RK), green kiwifruit (GRK), and gold kiwifruit (GOK) and determine the effects of various packaging methods on the quality of chicken breast injected with various kiwifruit types during storage. The physicochemical and textural properties of chicken breasts containing various kiwifruit types were evaluated. Chicken breasts were injected with brine solution containing 5% kiwifruit powder, and their product quality was measured every 3 days until day 9. GOK and RK addition to chicken breast delayed oxidation during storage. Adding GOK and GRK to chicken breast was found to increase tenderness. Modified‐atmosphere packaging (MAP) delayed microbial growth more effectively than over‐wrap packaging (OWP). These findings suggest that the combination of GOK and MAP potentially extends shelf‐life without deteriorating quality.

Properties of encapsulated raspberry powder and its efficacy for improving the pH, colour quality and shelf life of modified atmosphere packaged chicken nuggets

ABSTRACT 1. Recent research has shown that encapsulated raspberry powder (RP) is a natural colourant for foodstuffs. However, no research has been conducted on its use in chicken nuggets. In addition, the effect of RP on products with and without phosphate addition is unknown. This study assessed the effects of RP (control, 0.5%, 1.0%) and phosphate (0.0%, 0.3%) on the pH and colour quality properties of nuggets. 2. In the production of RP, red raspberry (Rubus ideaus L.) juices were encapsulated using maltodextrin in a spray-dryer. Antioxidant activity, total anthocyanin, total phenolics, colour, moisture and pH analyses of the RP were performed. 3. Nuggets were packaged in modified atmosphere packaging (MAP; 40%CO2 + 60%N2) and were stored at 2.0 ± 0.5°C for 120 d. The pH and external and internal surface colour (L*, a*, b*, C* and h) values were measured on d 0, 15, 30, 45, 60, 75, 90, 105 and 120 of storage. 4. The addition of phosphate increased the pH in the samples, while these decreased with the addition of RP (p < 0.05). During storage, the highest pH were seen in the phosphate samples and the lowest in the nuggets with 1.0% RP addition (p < 0.05). 5. With the addition of phosphate, the external surface a* value of nuggets increased (p < 0.05). Depending on the level of RP added to the nuggets, the external surface L* value decreased and a* and b* values increased (p < 0.05). After d 30 of storage, the a* value increased in the samples with RP addition and this increase was higher in the with phosphate nuggets (p < 0.05). 6. The internal surface a* value increased with the addition of RP during nugget production (p < 0.05). The increase in a* value was greater in samples with added phosphate (p < 0.05). During storage, the highest a* values were seen in nuggets treated with phosphate + 0.1% RP (p < 0.05). The addition of RP to chicken nugget emulsion improved redness, colour stability and shelf life.

Enterococcus spp. in fish: Analysis of the presence and resistance in samples from Tri-City, Poland.

The growing concern over antibiotic resistance in foodborne pathogens necessitates comprehensive assessments of its prevalence and associated risks in various food products. The present study aimed to assess the occurrence of Enterococcus spp. in samples of fish purchased at various points of sale in the Tricity region. The selection of products (n = 74) was based on their availability and included both fish caught in the Baltic region and products imported from, Vietnam, China, Norway, and European Union (EU) countries. For bacterial isolation, samples were inoculated into selective broth, and the growth of enterococci was assessed based on turbidity. Positive cultures were confirmed by a change in color in bromocresol purple broth and were isolated on Slanetz-Bartley agar. Bacteria were present in all tested samples regardless of the degree of raw material processing as follows: frozen (F)- 55%, fresh/raw (FS)- 70.6%, thawed (DF)- 30%, smoked (S)- 50%, and the packaging methods, modified atmosphere packaging (MAP)- 34.4%, unit packaging (UP)- 75%, and sold in bulk (SB)- 76.9%, with an overall frequency of occurrence of 58.1%. The number of bacteria ranged from not detected to 4.28-log cfu/g, with the lowest mean values for thawed fish and those packed in MAP. Tests conducted on 24 strains isolated from samples showed their varied sensitivity to tetracyclines. Single cases of multidrug resistance of the tested strains were also observed. The conducted statistical analysis did not show statistically significant differences in the count of enterococci based on the origin, degree of processing, or packaging (p < 0.05). Moreover, differences in strain sensitivity to ampicillin were observed. Detected cases of resistance, especially to tetracycline, require careful monitoring and action to limit the health risks associated with resistant bacterial strains in food products.

INNOVATIVE TECHNOLOGIES OF POST-HARVEST PROCESSING OF VEGETABLES AND FRUITS

Purpose. To analyze the current use of edible coatings for post-harvest processing of vegetables and fruits aimed at reducing losses and maintaining quality during storage. Results. Analysis of recent Ukrainian and foreign scientific and patent literature showed that the main advantages of coatings were based on such features as cost, availability, functionality, mechanical properties, optical properties, barrier capacity against gas flow, structural resistance to water and microorganisms, and sensory acceptability. They are used in combinations with bioactive compounds that confer additional functions on foods and extend their shelf lives. Conclusion. Transpirable coatings of fruits and vegetables slow down the post-harvest ripening processes in fresh products, preventing their further degradation and rotting. They are easier to use in packaging plants, require less investment and even outperform state-of-the-art packaging such as modified atmosphere packaging. Such protection mostly does not entail toxicological problems. Such coatings facilitate manufacturing and transport companies' work. Crops can stay in the field longer, continue to ripen, and can be harvested later because the risk of rot during transport and retail is reduced. In this way, the waste amount throughout agricultural production in general is decreased.

Unravelling the Influence of Perforation Sizes on Physicochemical, Sensory and Microbial Attributes of Modified Atmosphere Packaged Refrigerated Chicken Patties

ABSTRACTThe changes in lifestyle patterns and the adoption of a busy life have led to the widespread acceptance of ready‐to‐cook (RTC) products, particularly meat products. Chicken patties, being a preprocessed RTC product, are consumed worldwide. This study investigates the impact of perforated modified atmosphere packaging (MAP) on the physicochemical, sensory and microbial attributes of chicken patties stored at 4 °C for 14 days. The focus is on assessing the consequences of package damage occurring at any stage of the supply chain. Perforations measuring 0.2 mm and 0.4 mm were introduced into polypropylene packaging boxes, wherein chicken patties were subsequently packaged under five distinct gas concentrations of CO2 (ranging from 0% to 50%) and O2 (ranging from 0% to 50%), alongside a constant level of 50% N2. A control group containing normal air along with perforations was included for comparison with other groups containing modified atmospheres. The results indicated that the package (PPCON9) with the highest concentration of CO2 (50%) and the lowest O2 (0%) with 0.2 mm perforations was the most effective in preserving the quality attributes of chicken patties during storage by reducing moisture loss (3%), protein denaturation (0.38%), lipid oxidation (0.54%), cooking loss (0.72%), shrinkage (0.18%), water holding capacity (5.67%), sensory losses and microbial spoilage. The highest losses occurred in the package with 0.4 mm perforations containing normal air (PPA2). The study highlights that even if a package containing a modified atmosphere is damaged, it still efficiently preserves the chicken patties compared to a damaged package containing normal air. However, leakage significantly impacts the efficiency of MAP, necessitating effective solutions to prevent losses that occur from this issue.

A Microbiological and Sensory Evaluation of Modified Atmosphere-Packed (MAP) Chicken at Use-By Date and Beyond.

Consumers are responsible for a large proportion of food waste, and food that has reached its use-by or best-before date is often discarded, even if edible. In this study on fresh chicken, the suitability of use-by dates currently used in the EU was evaluated by using microbial and sensory analyses. This was carried out by analyzing bacterial populations of chicken breast fillets (M. pectoralis major) at three different time points (use-by date, 2 days past use-by date, 4 days past use-by date) and two different storage temperatures (4 °C, 8 °C). A discrimination triangle test was performed to check for sensory differences between chicken breast fillets cooked at the three selected time points for both storage temperatures. A consumer preference test was also performed for chicken breast fillets that had been stored at the highest recommended temperature (4 °C) and after being cooked at the three time points. Changes in populations of total aerobic count (TAC), Enterobacteriaceae (EB), and lactic acid bacteria (LAB) were recorded over time. Despite large differences in bacterial counts at the selected time points, with TAC populations of approximately 6.5 and 8.0 log CFU/g at use-by date and four days after use-by date, respectively, storage for two or four extra days had no significant effect on the sensory parameters of cooked chicken compared with chicken consumed at its use-by date. Since the TAC populations were close to or above levels that are associated with spoilage, more work is needed to explore if shelf life can be extended.

Effect of VP, MAP and combined packaging systems on the physicochemical properties and microbiological status of veal from unweaned calves

The packaging system is one of the factors influencing the preservation of the nutritional value, microbiological safety, and sensory attributes of meat. The study investigated changes in physicochemical and microbiological properties taking place during 15-day refrigerated storage of two calf muscles, the longissimus lumborum (LL) and semitendinosus (ST), packaged in three systems, respectively, vacuum packing (VP), modified atmosphere packaging (MAP, 80% O2 + 20% CO2), and a combined system (VP + MAP, 8 d in VP followed by 7 d in MAP). LL and ST stored in VP had significantly lower levels of lipid oxidation, higher α-tocopherol content, and higher instrumentally measured tenderness in comparison with the samples stored in MAP. On the other hand, the MAP samples had lower purge loss at 5 and 15 days, a higher proportion of oxymyoglobin up to 10 days of storage, and a better microbiological status. Calf muscle samples stored in the VP + MAP system had intermediate values for TBARS and α-tocopherol content and at the same time were the most tender and had the lowest counts of Pseudomonas and Enterobacteriaceae bacteria at 15 days. All packaging systems ensured relatively good quality of veal characteristics up to the last day of storage. However, for MAP at 15 days of storage, unfavourable changes in colour (a high level of metmyoglobin and a decrease in oxymyoglobin, redness and R630/580 ratio) and in the lipid fraction (a high TBARS value and a significant decrease in α-tocopherol content) were observed.

Monitoring the Bioprotective Potential of Lactiplantibacillus pentosus Culture on Pathogen Survival and the Shelf-Life of Fresh Ready-to-Eat Salads Stored under Modified Atmosphere Packaging.

Globally, fresh vegetables or minimally processed salads have been implicated in several foodborne disease outbreaks. This work studied the effect of Lactiplantibacillus pentosus FMCC-B281 cells (F) and its supernatant (S) on spoilage and on the fate of Listeria monocytogenes and Escherichia coli O157:H7 on fresh-cut ready-to-eat (RTE) salads during storage. Also, Fourier transform infrared (FTIR) and multispectral imaging (MSI) analysis were used as rapid and non-destructive techniques to estimate the microbiological status of the samples. Fresh romaine lettuce, rocket cabbage, and white cabbage were used in the present study and were inoculated with L. pentosus and the two pathogens. The strains were grown at 37 °C for 24 h in MRS and BHI broths, respectively, and then were centrifuged to collect the supernatant and the pellet (cells). Cells (F, ~5 log CFU/g), the supernatant (S), and a control (C, broth) were used to spray the leaves of each fresh vegetable that had been previously contaminated (sprayed) with the pathogen (3 log CFU/g). Subsequently, the salads were packed under modified atmosphere packaging (10%CO2/10%O2/80%N2) and stored at 4 and 10 °C until spoilage. During storage, microbiological counts and pH were monitored in parallel with FTIR and MSI analyses. The results showed that during storage, the population of the pathogens increased for lettuce and rocket independent of the treatment. For cabbage, pathogen populations remained stable throughout storage. Regarding the spoilage microbiota, the Pseudomonas population was lower in the F samples, while no differences in the populations of Enterobacteriaceae and yeasts/molds were observed for the C, F, and S samples stored at 4 °C. According to sensory evaluation, the shelf-life was shorter for the control samples in contrast to the S and F samples, where their shelf-life was elongated by 1-2 days. Initial pH values were ca. 6.0 for the three leafy vegetables. An increase in the pH of ca. 0.5 values was recorded until the end of storage at both temperatures for all cases of leafy vegetables. FTIR and MSI analyses did not satisfactorily lead to the estimation of the microbiological quality of salads. In conclusion, the applied bioprotective strain (L. pentosus) can elongate the shelf-life of the RTE salads without an effect on pathogen growth.

Untargeted volatolomic approach for the identification of sensory quality markers of fresh-cut iceberg lettuce stored in modified atmosphere packaging

Fresh-cut iceberg lettuce is appreciated by consumers for its nutritive properties, convenience, and ease of use. Producers are then pushed to preserve the quality of these products by using modified atmosphere packaging (MAP). In this study, the influence of passive MAP (air) or active MAP (1–5 % O2), on several quality attributes of fresh-cut iceberg lettuce samples was evaluated during storage time (from 3 to 14 d) at 4 °C. The respiration rate and the electrolyte leakage values of samples packaged in active MAP were higher than those of fresh-cut iceberg lettuce stored in the air, indicating stressful storage conditions. Inverse trends were observed for O-quinones content and browning index until the end of the storage period. Sensory analysis highlighted that active MAP has preserved the visual quality, limiting the browning of the cut surfaces. On the other hand, active MAP modified the sensory profile after 3 d at 4 °C, and samples scored below the marketability limit for both odor and off-odor. Finally, a volatile organic compound characterization by headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC–MS) allowed the identification of target molecules that could signal a decrease in food quality under different conditions. A total of 118 compounds were identified and, among them, ethyl alcohol and acetaldehyde were identified as the best quality markers for passive MAP and active MAP samples, respectively, while ethyl acetate could represent a good compromise to assess the quality of fresh-cut iceberg lettuce, regardless of MAP conditions.

Analysis of quality-related proteins in golden pompano (Trachinotus ovatus) fillets with modified atmosphere packaging under superchilling storage

Here, we aimed to study the changes in proteome of golden pompano fillets during post-mortem storage. Tandem mass tags (TMT)-labeled quantitative proteomic strategy was applied to investigate the relationships between protein changes and quality characteristics of modified atmosphere packaging (MAP) fillets during superchilling (-3 ℃) storage. Scanning electron microscopy was used to show that the muscle histology microstructure of fillets was damaged to varying degrees, and low-field nuclear magnetic resonance was used to find that the immobile water and free water in the muscle of fillets changed significantly. Total sulfhydryl content, TCA-soluble peptides and Ca2+-ATPase activity also showed that the fillet protein had a deterioration by oxidation and denaturation. The Fresh (FS), MAP, and air packaging (AP) groups were set. Total of 150 proteins were identified as differential abundant proteins (DAPs) in MAP/FS, while 209 DAPs were in AP/FS group. The KEGG pathway analysis indicated that most DAPs were involved in binding proteins and protein turnover. Correlation analysis found that 52 DAPs were correlated with quality traits. Among them, 8 highly correlated DAPs are expected to be used as potential quality markers for protein oxidation and water-holding capacity. These results provide a further understanding of the muscle deterioration mechanism of packaging golden pompano fillets during superchilling.

Osmotic assisted convective drying of pomegranate arils: Process optimisation, structural characterisation, and bioactive compound evaluation

Convective drying of pomegranate arils is time-consuming, energy-intensive, and affects the quality. Osmotic pre-treatment has been resorted prior to convective drying to improve drying performance and quality. In the present work, the osmosis factors such as temperature (30 - 60°C), time (50 - 250 min), and total soluble solids (TSS) (40 - 60°B) were studied using Box-Behnken design of response surface methodology. The quadratic models obtained adequately explained the process, and the optimum conditions were temperature (48.52°C), time (209.65 min), and TSS (51.31°B). The osmotic pre-treatment at optimum conditions followed by the convective tray drying reduced the drying time by nine hours, and the mean energy consumption by 0.172 MJ/g. Light microscopy revealed rupture and breakage of the honeycomb-like cellular structure of the pomegranate aril. Quality analysis of dried arils revealed that the texture (softness) and TSS improved by 11.75 N and 4.2°B, respectively. Likewise, sensorial quality parameters such as taste, mouth feel, and overall acceptability of the OATD (osmo-assisted tray-dried) arils significantly improved over the TD (tray-dried). However, there was a minor higher loss of 15.48, 12.52, and 15.88% in anthocyanin, phenols, and antioxidant capacity in OATD compared to TD. The OATD arils can be stored for up to six months in a modified atmosphere package (MAP).

Effects of combined soluble gas stabilization, modified atmosphere packaging and cold plasma on controlling chilled chicken breasts spoilage

Effects of combined soluble gas stabilization, modified atmosphere packaging and cold plasma on controlling chilled chicken breasts spoilage

Tailored antimicrobial PHBV-based packaging for extended shelf life of processed cheese

A coupled experimental and modelling approach was used to develop an adapted PHBV active film with Lauroyl Arginate Ethyl (LAE®) as an antimicrobial compound incorporated in the bulk or as a coating to prolong the shelf life of cheese. The minimum inhibitory concentration (MIC) of LAE® was evaluated in the range of 25–100 ppm against spoilage and pathogenic microorganisms, such as Micrococcus luteus, Penicillium roqueforti, Salmonella enteritidis, and Pseudomonas putida. Film production induced a loss of 50 % of LAE® when introduced into the bulk, whereas only 4 % was lost in the case of incorporation by coating. When in contact with food simulant D1 (50 % ethanol), a release of 43 % and 34 % of LAE® was achieved in 10 days at 20 °C for LAE® incorporated as a coating and in the bulk, respectively, while a lower release level of 34 % and 14 % respectively was observed in food simulant C (20 % ethanol), confirming the interest to use active coating for fatty foods. The developed packaging was well adapted to reach the MIC while remaining below the admissible daily intake. The results also showed that the PHBV film activated with LAE® incorporated as a coating was as effective as modified atmosphere packaging (MAP) to preserve cheese against microorganism growth.

Psychrotrophic Bacteria and Yeast Populations in Turkish White and Tulum Cheeses

In this study, pH and microbiological (psychrotrophic bacteria and yeast) properties of White and Tulum cheeses were investigated. According to the results of our study, the lowest pH value identified was 4.42, and the highest pH was 5.84 in the cheese samples. Psychrotrophic bacteria counts ranged from 4.15 to 6.21 log CFU/g for White cheese and 4.02 to 6.15 log CFU/g for Tulum cheese. Psychrotrophic yeast counts ranged from 2.98 to 7.58 log CFU/g for White cheese and 2.99 to 6.77 log CFU/g for Tulum cheese. The highest values of psychrotrophic yeast were detected in the Tulum cheese samples. As a result, it was determined that the numbers of psychrotrophic bacteria and yeast were high in both Tulum and White cheese samples. These high numbers of psychrotrophic microorganisms during cheese storage in the refrigerator may increase even further and reduce shelf life. To extend the shelf life of these cheeses, it is recommended to ensure hygienic handling practices and explore the use of natural preservatives or modified atmosphere packaging.

Enhancing the Quality of Ripened ‘Irwin’ Mangoes and the Shelf Life through Chitosan Edible Coating and Modified Atmosphere Packaging

Enhancing the Quality of Ripened ‘Irwin’ Mangoes and the Shelf Life through Chitosan Edible Coating and Modified Atmosphere Packaging

Effect of modified atmosphere packaging (MAP) on quality and shelf-life of cucumber during cold storage

Effect of modified atmosphere packaging (MAP) on quality and shelf-life of cucumber during cold storage

Effect of Potassium Metabisulphite and Modified Atmosphere Packaging on Shelf Life of Pink Oyster Mushroom (Pleurotus eous)

Effect of Potassium Metabisulphite and Modified Atmosphere Packaging on Shelf Life of Pink Oyster Mushroom (Pleurotus eous)

New approaches in pear preservation: Putrescine and modified atmosphere packaging applications to maintain fruit quality during cold storage

AbstractPear (P. communis L.), which is a climacteric fruit species, has a very short storage and shelf life, and significant losses occur due to high metabolic activity and the fruit's respiration rate after harvest. Therefore, preventing or reducing post‐harvest quality losses in pear is one of the most basic problems awaiting solution. In this study, we planned for this purpose; the fruits of the Ankara pear cultivar treated with modified atmosphere packaging (MAP), putrescine (1 mM), and MAP + putrescine were stored for 120 days at 1°C and 90 ± 5% relative humidity. The quality analyses and measurements, such as weight loss, decay rate, fruit firmness, soluble solids content (SSC), titratable acidity (TA), total phenolic compounds, antioxidant capacity, organic acids, and specific phenolic compounds, were performed on the 30th, 60th, 90th, and 120th days. Weight loss and decay ratios were lower for putrescine and putrescine + MAP‐applied fruit. With these applications, the softening of the fruit was slowed down, and the increasing SSC in the fruit and the decreasing TA rates were lower, and thus the ripening of the fruit was delayed. Changes in individual phenolic content and organic acids were lower in MAP and putrescine‐applied fruit. The study revealed that MAP and putrescine applications in pear can be used effectively to maintain fruit quality after harvest.

Impact of Different Technologies and Methods to Increase the Shelf Life and Maintain the Sulforaphane Content in Broccoli: A Review

Broccoli, a nutrient-rich vegetable known for its sulforaphane content and health benefits, faces challenges related to its short shelf life and susceptibility to spoilage. This study explores various techniques to maintain sulforaphane content and extend broccoli's shelf life. Freezing, although effective for long-term preservation, involves blanching that inactivates myrosinase, preventing sulforaphane formation. Cold storage at 4-5°C slows respiration and senescence, extending shelf life up to 14 days while preserving bioactive compounds. Modified atmosphere packaging (MAP) enhances shelf life by altering the package atmosphere, with active packaging using 15% CO2 and 7.5% O2 at 0°C proving particularly effective in reducing bacterial growth and preserving quality. Light exposure, especially with yellow LED lights and moderate UV treatment, retains high levels of glucosinolates and phenolic compounds. Irradiation effectively eliminates pathogens and preserves visual quality, with gamma irradiation slowing the oxidation of phenols and ascorbic acid, although further research is needed on its sensory and nutritional effects. High-pressure processing (HPP), a non-thermal method, significantly boosts sulforaphane bioactivity by converting glucosinolates at pressures up to 600 MPa, despite challenges such as high costs and operational safety, and prevents microbial growth, extending shelf life while maintaining nutritional quality. Additionally, techniques like steaming for optimal durations, microwaving at high power for short times, and stir-frying help preserve sulforaphane content during cooking. Preharvest chemical treatments like chitosan and calcium also enhance broccoli's defence mechanisms and bioactive compound levels. Overall, these methods offer a comprehensive approach to preserving sulforaphane content and extending broccoli's shelf life, providing valuable insights to enhance the storage, quality, and health benefits of broccoli for consumers.

Effect of Packaging Methods and Storage Temperatures on the Quality of Erzurum Cheese Halva

Erzurum cheese halva is a traditional food registered with a geographical indication with its own taste and flavor, which is produced with saltless fresh civil cheese, fresh cream and flour mixture. This study was conducted to investigate the changes in the physical, chemical and microbiological properties of Erzurum cheese halva in different packaging and storage tempareture. The produced cheese halva samples were stored for two months in two different packaging methods (aerobic and vacuum packaged) and at three different storage temperatures (room temperature, +4℃ and -18℃). Some microbiological, chemical, physicochemical and sensory analysis of cheese halva samples were investigated during storage period. Furthermore, the dry matter, ash, protein, fat, salt and aw contents of cheese halva samples were determined on the 0th day of storage. The most obvious sign of deterioration were moldiness on aerobic packaged samples both the room temperature and +4℃ stored, and they were spoiled on the10th and 15th day of storage, respectively. It was observed that the acidity has increased and consumability has decreased in vacuum packaged samples stored at +4°C. Although neither chemical nor microbiological deterioration occurred in the vacuum packaged cheese halva samples stored on -18°C, negatively affects in the fibrous structure of the product and reduces the overall acceptability of the product. For this reason, future studies must be carried out that is necessary to search for more suitable packaging methods (e.g. modified atmosphere packaging) to protect the product's typical textural structure during the shelf life.