Background: Acute appendicitis is one of the most common surgical emergencies. Accurate diagnosis ofacute appendicitis is based on careful history, physical examination and laboratory and imaging findings.Objective: The primary aim is the correlation between clinical, biochemical and radiological assessment inpatients of appendicitis and also to reduce the rates of negative appendicectomy.Method: The study was carried out in Department of General Surgery, Sir Sunderlal Hospital, IMS and BHUfrom September 2015 to July 2017. Total 61 patients of age group 16-65 years and either sex were evaluatedon the basis of predetermined proforma, which included a detailed history, clinical examination, laboratoryinvestigations and high resolution sonography or CT and histopathology. Sensitivities, specificities, positiveand negative predictive values of TLC, neutrophil percentage, Modified Alvarado Score, CRP, D-dimer,USG and CT scan were calculated in respect to histopathology finding as a gold standard.Results: Modified Alvarado scoring (MAS 7-9:Appendicitis definitive) was present in 24 (39.3%) patients.Raised leucocyte count was present in 49 (80.32%) patients, while neutrophil count above 75% waspresent in 47 (77%) patients. Raised CRP was present in 55 (90.16%) patients with cut-off of >3 mg/l(normal range 1-3 mg/l) while D-dimer was elevated in 44 (72.13%) patients with cut-off of >5 mg/l. Onultrasonography,(77%) patients were diagnosed as acute appendicitis whereas (83.3%) had diagnosis ofacute appendicitis in CECT abdomen. Histopathological examination was positive in 60 (98.4%) patients.Conclusion: We concluded that combination of thorough clinical evaluation along with certain routinebiochemical & specific markers and ultrasonography as the primary imaging modality is sufficient inestablishing a diagnosis of acute appendicitis in more than 90% cases.