Modification Of Response Research Articles

Non-concordant epigenetic and transcriptional responses to acute thermal stress in western mosquitofish (Gambusia affinis).

Climate change is intensifying the frequency and severity of extreme temperatures. Understanding the molecular mechanisms underlying the ability to cope with acute thermal stress is key for predicting species' responses to extreme temperature events. While many studies have focused on the individual roles of gene expression, post-transcriptional processes and epigenetic modifications in response to acute thermal stress, the relative contribution of these molecular mechanisms remains unclear. The wide range of thermal limits of western mosquitofish (Gambusia affinis) provides an opportunity to explore this interplay. Here, we quantified changes in gene expression, alternative splicing, DNA methylation and microRNA (miRNA) expression in muscle tissue dissected from mosquitofish immediately after reaching high (CTmax) or low thermal limit (CTmin). Although the numbers of genes showing expression and splicing changes in response to acute temperature stress were small, we found a possibly larger and non-redundant role of splicing compared to gene expression, with more genes being differentially spliced (DSGs) than differentially expressed (DEGs), and little overlap between DSGs and DEGs. We also identified a small proportion of CpGs showing significant methylation change (i.e. differentially methylated cytosines, DMCs) in fish at thermal limits; however, there was no overlap between DEGs and genes annotated with DMCs in both CTmax and CTmin experiments. The weak interplay between epigenetic modifications and gene expression was further supported by our discoveries of no differentially expressed miRNAs. These findings provide novel insights into the relative role of different molecular mechanisms underlying immediate responses to extreme temperatures and demonstrate non-concordant responses of epigenetic and transcriptional mechanisms to acute temperature stress.

Abstract 6517: ALDOC as an indicator of HMGB1 blockade combined with checkpoint inhibitor based immunotherapy in GBM patients

Abstract Aldolase is an enzyme that takes part in glycolysis by catalyzing a six-carbon intermediate to yield two three-carbon products, requiring ATP. The aldolase family comprises three members, namely ALDOA, ALDOB, and brain-specific ALDOC. Low-grade gliomas and glioblastoma (GBM) are characterized by shared genetic events, including chromosome 1p/19q codeletion, EGFR amplification, and ATRX loss of function, in which ALDOC plays a role. Additionally, ALDOC is responsive to IDH1 mutations. If patients display IDH1 mutations, it is expected that ALDOC performance will be relatively higher. This finding suggests a more favorable prognosis. The ALDOC promoter region's function is impeded by hypermethylation, which declines as glioma progresses. The regulatory mechanisms and implications remain unclear. We established a multi-omics profile and identified several crucial mechanisms activated upon the loss of ALDOC expression. Among these mechanisms, we found HMGB1 to have a key role in linking the immune response, tumor microenvironment, and epigenetic modifications. We compared the proteomic and secretomic platforms in an ALDOC overexpression/knockdown model, which showed abundant HMGB1 expression following ALDOC inhibition. Importantly, there is an inverse correlation between ALDOC expression and HMGB1 protein levels. Additionally, our findings reveal that upregulation of ALDOC promotes its translocation to the nucleus, thereby enhancing its binding affinity with HMGB1. This, in turn, enables extracellular release of HMGB1 in GBM, which triggers signaling via receptors for advanced glycation end-products (RAGE) and several cytokines. Moreover, HMGB1 remodels the immune microenvironment and upregulates multiple immune checkpoint molecules, including PD-L1, PD-L2, B7-H3, and MHCs. Not all GBM models exhibited significant effects when combined with immune checkpoint blockade using HMGB1 blockade. However, ALDOC knockout mice demonstrated poorer survival and treatment efficacy, suggesting that ALDOC may be crucial for a personalized immunotherapy strategy. Citation Format: Yi-Fang Yang, Ming-Hsien Chan, Chi-Long Chen, Michael Hsiao, Yu-Chan Chang. ALDOC as an indicator of HMGB1 blockade combined with checkpoint inhibitor based immunotherapy in GBM patients [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 6517.

Determination of response modification coefficient of SPSW in RC frame using plastic design method

ABSTRACT In the last few decades, the idea of using thin steel plate shear walls (SPSW) as a resistant system against lateral loads in designing and reinforcing buildings has been of concern to researchers and designers. In this research, we studied the response modification coefficient (R), the overstrength factor (Ω0), and the deflection amplification factor (C d ) in a reinforced concrete special moment frame (RC-SMF) with thin SPSW. We used the performance-based plastic design (PBPD) method. For this purpose, we consider frames with SPSW systems with different story numbers. Static pushover analysis is performed on these frames using the strip model in OpenSees software. OpenSees software is the Open System for Earthquake Engineering Simulation located at http://opensees.berkeley.edu. Simulation results were compared with experimental results, and acceptable matching was observed between the two. Finally, the values of response modification coefficient (R), overstrength factor (Ω0), and deflection amplification factor (C d ) for RC-SMF with thin SPSW were calculated based on the Uang method. The ultimate limit design method proposed them as 9.37, 2.21, and 11.06.

Protective face masks affect clearly produced diphthongs by L1 and L2 English talkers

The study investigated how the use of protective face masks and language experience shape the production of listener-oriented clear speech. One L1 and one L2 English talker read sentences in a clear and conversational speaking style with and without a surgical mask. Formant trajectories between the onset and offset of two diphthongs, /aɪ/ and /eɪ/, were analyzed using Euclidean distance in the F1–F2 vowel space. The results showed that the distance between the onset /a/ and the offset /ɪ/ was larger when speech was produced without the mask and when speaking clearly. These modifications were larger for L1 talker compared to the L2 talker. The Euclidian distance for /eɪ/was only affected by speaking style. The results suggest that talkers produced hyperarticulated diphthongs characterized by larger formant movements in clear speech. The presence of a mask limited jaw movement for the diphthong containing the low vowel. Additionally, the L1 talker made larger articulatory modifications in response to the presence of a mask and listener-oriented clear speech compared to the talker with less extensive experience with the target language. These production patterns may be related to lower word recognition in noise for masked, conversational, and L2 speech found in previous work.

Characteristics of torsional ground motions obtained for Turkey and their appraisal against Eurocode 8

In current engineering practice, the torsional effects of ground motions are only considered implicitly through “accidental eccentricity coefficients". In seismic design codes, these coefficients account for not only the unintended (or uncertain) asymmetric distributions of structural mass and stiffness in the plan but also the torsional components of ground motions. The torsional effects may develop due to wave passage effects or the incoherency of translational ground motions along the foundation systems. While it is possible to obtain torsional components of ground motions directly from seismic records with modern instruments, such sensors are uncommon. Therefore, several numerical procedures have been proposed in the literature for estimating torsional components of ground motions from translational measurements. In this study, the Single Station Procedure (SSP)—based on the deconstruction of translational ground motions into body waves and the subsequent reconstruction of rotational ground motions—is employed to obtain torsional ground motions for seven different seismic events in Turkey. Additionally, the corresponding accidental eccentricity coefficients that can cause the same response modification with torsional ground motions are determined. First, a conventional procedure is utilized to obtain the corresponding accidental eccentricity coefficients. Various drawbacks of the conventional procedure, such as negative eccentricity coefficients, are addressed. Then, a relatively obscure definition of accidental eccentricity is revived to propose a novel procedure for determining accidental eccentricity coefficients. It is observed that the proposed procedure yields higher values for the accidental eccentricity coefficients than the conventional procedure. It also observed that the torsional spectrum provided in Eurocode 8 provisions requires higher eccentricity values than those implied by the recorded ground motions from Turkey.

Fungal Cell Wall-Associated Effectors: Sensing, Integration, Suppression, and Protection.

The cell wall (CW) of plant-interacting fungi, as the direct interface with host plants, plays a crucial role in fungal development. A number of secreted proteins are directly associated with the fungal CW, either through covalent or non-covalent interactions, and serve a range of important functions. In the context of plant-fungal interactions many are important for fungal development in the host environment and may therefore be considered fungal CW-associated effectors (CWAEs). Key CWAE functions include integrating chemical/physical signals to direct hyphal growth, interfering with plant immunity, and providing protection against plant defenses. In recent years, a diverse range of mechanisms have been reported that underpin their roles, with some CWAEs harboring conserved motifs or functional domains, while others are reported to have novel features. As such, the current understanding regarding fungal CWAEs is systematically presented here from the perspective of their biological functions in plant-fungal interactions. An overview of the fungal CW architecture and the mechanisms by which proteins are secreted, modified, and incorporated into the CW is first presented to provide context for their biological roles. Some CWAE functions are reported across a broad range of pathosystems or symbiotic/mutualistic associations. Prominent are the chitin interacting-effectors that facilitate fungal CW modification, protection, or suppression of host immune responses. However, several alternative functions are now reported and are presented and discussed. CWAEs can play diverse roles, some possibly unique to fungal lineages and others conserved across a broad range of plant-interacting fungi. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.

COMPARISON BETWEEN SMF AND SPSW STEEL BUILDING FOR MIXED-USE RESIDENCE USING THE AHP METHOD

The objective of this work is to compare the Special Moment Frame (SMF) system and the Steel Plate Shear Wall (SPSW) system using the AHP multi-criteria comparison method with the two Seismic Load Resisting Systems (SFRS). From the linear static analysis, dynamic analysis, and nonlinear static analysis (Pushover), representative characteristics of each Seismic Force-Resisting System (SFRS) are determined. These characteristics include the structure's self-weight, target displacement, floor drifts, and seismic response modification factor (R). As a result, two buildings are obtained with different SFRS that comply with the design of their elements and checks according to the regulatory requirements. After determining the capacity curve of each Seismic Force-Resisting System (SFRS) and assessing the performance level of the buildings, the conclusion is drawn that the Special Plate Shear Wall (SPSW) system is more suitable and advantageous compared to the Special Moment Frame (SMF) system. This preference is due to the SPSW system's ability to provide ductility, which translates into higher energy dissipation capacity, and stiffness, which leads to reduced floor drifts in the building.

Quantification of seismic performance factors of mixed concrete/steel buildings using the FEMA P695 methodology

The concurrent utilization of both concrete and steel stories within buildings results in the establishment of a hybrid lateral load-resistant system throughout its height. Different seismic codes do not provide specific values for the seismic factors to be used in the design of mixed concrete/steel buildings. Through these parameters, the nonlinear responses of the structure are estimated. In this study, an investigation was conducted into the seismic design parameters' quantification, encompassing the response modification factor (R), the system over-strength factor (Ωo), and the deflection amplification factor (Cd). Whether to apply the transition story or not in the transition zone between the concrete and steel sections along with considering the different ratios between the number of concrete-based stories and steel-based stories, 18 archetypes in the form of three performance groups of 7, 13, and 19 stories were studied. Pushover curves of all archetypes were achieved. Then, to evaluate the system's behavior and measure the adequacy of the assumed R factor, Incremental Dynamic Analysis (IDA) was used and fragility curves were also extracted to investigate the collapse probability of different archetypes. The results showed that the values of R = 8, Ω0 = 3, and Cd = 5.5 were suitable for the mixed concrete/steel buildings studied. Therefore, a unique R can be assigned to archetypes in different concrete-to-steel floor ratios and the two transition methods. Taking overall uncertainty into account, R= 8 was entirely adequate to satisfy the acceptance criteria of the FEMA P695 methodology.

Application of Endurance Time Method in Seismic Assessment of RC Frames Designed by Direct Displacement-Based Procedure

This paper addresses the Direct Displacement-Based Design (DDBD) approach of multi-story RC frame structures consistent with changes ‎to design criteria between Turkish earthquake codes of TSC-2007 and TBEC-2018. The corresponding response modification factor (R) of structures designed based on the DDBD approach is also estimated in this research. The design base shear forces of both codes are compared considering different R factors and also with that of the DDBD approach. The results showed that the DDBD approach, as per TBEC-2018, provides RC frame structures with higher R values compared to the similar approach in accordance with TSC-2007. The Endurance Time (ET) method is a time history-based procedure for seismic assessment of ‎structures ‎under intensifying dynamic excitations aided to judge their performance at various intensity levels. Since, up to now, the ET method has not been considered to evaluate the performance of the structures designed by the DDBD approach, this paper addresses this issue. The ET ‎performance curves of RC frames show that structures designed by the DDBD approach in ‎accordance with TBEC-2018 exhibit higher Interstory Drift Ratios (IDRs) values than TSC-2007 at various hazard levels.

Retrofitting of Damaged Crumb Rubber Concrete Frame Using Fiber Reinforced Polymers

Abstract: The research work is focused on the retrofitting of damaged crumb rubber concrete frame. The retrofitting of these frames was carried out using Carbon Fiber Reinforced Polymers (CFRP) wrapping technique accompanied with beam weakening technique, carried out at certain locations to achieve the desired failure mechanism. Two previously tested 1/3rd reduced scale models Crumb rubber concrete (CRC) and Normal concrete (NC) were CFRP retrofitted along with beam weakening and then tested dynamically. From the as-built testing strong-beam weak-column effect was observed, for that purpose beams weakening through cores removal away from the face of the column equal to two times depth of the beam, so as to shift the damage from the brittle failure mechanism of column and joint damage to more ductile damage in the beam. In addition to beam weakening the damaged beam column joints were CFRP retrofitted. A comparative study between the retrofitted and as-built models was done. The results obtained from testing has recovered the ductility up to 82% and response modification factor up to 90% for normal concrete while in case of crumb rubber concrete frame the ductility is recovered up to 76% and its response modification factor up to 81%. As intended, a much-distributed damage behavior/cracking pattern as compared to the reference models, especially in the case of normal concrete model. The intended purpose of the retrofitting in recovering seismic properties and a comparatively distributed damage/cracking pattern was achieved. The purpose of beamweakening through core removal for shifting the damage from columns and joints towards the beams for achieving a more ductile failure mechanism was achieved to some extent.

NONLINEAR SIMULATIONS TO EVALUATE THE CODE-BASED RESPONSE MODIFICATION FACTOR FOR SEISMIC DESIGN OF SLAB-ON-PILE STRUCTURE

Slab on Pile (SOP) structure using concrete spun pile (CSP) is frequently constructed for highway or railway infrastructures in Indonesia. However, some previous studies showed that spun piles have low energy dissipation and ductility. Therefore, spun pile application as bridge piers, particularly in high seismic locations such as Indonesia, needs additional consideration. The response modification factor (R) in the SOP elastic seismic design needs to be carefully considered since this parameter reflects the ability of the structure to dissipate energy through inelastic behavior. The work discussed in this paper primarily concerns the evaluation of the R-value based on the Indonesia seismic bridge design code, SNI 2833-2016, which is applied to SOP seismic design. The study provided an alternative reference for engineers in the seismic design of a SOP structure. Preliminary elastic design of SOP structures demonstrated that the number of piles and structural flexibility is significantly affected by the R-values consideration. In addition, the pinching hysteresis captured from the nonlinear dynamic analysis results emphasized the limited capability of an SOP structure in dissipating energy under seismic excitation. Moreover, the spun pile concrete material, especially in the pile-to-pile head connection, was observed to have an earlier failure. This study strongly suggested using an R-value of 1.5 to achieve a high seismic performance SOP structure with little need for aftershock retrofitting, even though this would necessitate much more spun piles. However, some further research could be conducted to develop the high seismic performance but economical design of SOP structures.

Genome-wide identification of the N6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.)

BackgroundN6-methyladenosine (m6A) is one of the common internal RNA modifications found in eukaryotes. The m6A modification can regulate various biological processes in organisms through the modulation of alternative splicing, alternative polyadenylation, folding, translation, localization, transport, and decay of multiple types of RNA, without altering the nucleotide sequence. The three components involved in m6A modification, namely writer, eraser, and reader, mediate the abundance of RNA m6A modification through complex collaborative actions. Currently, research on m6A regulatory genes in plants is still in its infancy.ResultsIn this study, we identified 52 candidate m6A regulatory genes in common tobacco (Nicotiana tabacum L.). Gene structure, conserved domains, and motif analysis showed structural and functional diversity among different subgroups of tobacco m6A regulatory genes. The amplification of m6A regulatory genes were mainly driven by polyploidization and dispersed duplication, and duplicated genes evolved through purified selection. Based on the potential regulatory network and expression pattern analysis of m6A regulatory genes, a significant number of m6A regulatory genes might play important roles in growth, development, and stress response processes. Furthermore, we have confirmed the critical role of NtFIP37B, an m6A writer gene in tobacco, in enhancing drought resistance.ConclusionsThis study provides useful information for better understanding the evolution of m6A regulatory genes and the role of m6A modification in tobacco stress response, and lays the foundation for further elucidating the function of m6A regulatory genes in tobacco.

RNA N6-Methyladenosine Modification in DNA Damage Response and Cancer Radiotherapy.

The N6-methyladenosine (M6A) modification is the most common internal chemical modification of RNA molecules in eukaryotes. This modification can affect mRNA metabolism, regulate RNA transcription, nuclear export, splicing, degradation, and translation, and significantly impact various aspects of physiology and pathobiology. Radiotherapy is the most common method of tumor treatment. Different intrinsic cellular mechanisms affect the response of cells to ionizing radiation (IR) and the effectiveness of cancer radiotherapy. In this review, we summarize and discuss recent advances in understanding the roles and mechanisms of RNA M6A methylation in cellular responses to radiation-induced DNA damage and in determining the outcomes of cancer radiotherapy. Insights into RNA M6A methylation in radiation biology may facilitate the improvement of therapeutic strategies for cancer radiotherapy and radioprotection of normal tissues.

Bubble Sensors for Temperature Measurements through a Colorimetric Approach.

This paper introduces an innovative sensor utilizing bubbles coated with thermochromic paint, aiming to facilitate temperature measurements in challenging-to-reach locations without the requirement of an external power source. The research conducted is innovative in terms of both methodology and application. The characterization of the thermochromic properties of paints was, in fact, performed using spectroradiometric measurements by selecting a temperature range useful for applications in various fields including preventive conservation. The study encompasses two main objectives: (1) analyzing the color characteristics of thermochromic paint and plastic resin that forms the bubbles, and (2) assessing a temperature sensor comprising a thermochromic paint-coated bubble subjected to temperature variations. The thermochromic paint exhibits reversible color modifications in response to temperature changes, making it an ideal candidate for applications of this nature. The color characterization phase involves measurements using a spectroradiometer to compare the spectral reflectance factor (SRF%) of the colored plastic resin spread on canvas with that of the inflated bubbles. The sensor characterization entails evaluating color changes of the thermochromic paint on the bubble surface with varying temperatures. Experimental results indicate that the combination of a red (R) bubble and blue (B) thermochromic paint produces quantifiable color variations suitable for the proposed applications, whereas the alternative combination under examination, namely a blue bubble and red thermochromic paint, yields less accurate results. Considering that for both thermochromic paints the color change temperature is 35 °C, it is possible to see how, for B bubble with R thermochromic paint, the chromatic coordinates change value: C* = 3.14 ± 0.14 and h = 289.54 ± 11.58 at room temperature, while C* = 2.96 ± 0.12 and h = 304.20 ± 12.17 at 35 °C. The same is true for R bubble with B thermochromic paint where C* = 25.31 ± 1.01 and h* = 285.05 ± 11.40 at room temperature, while C* = 20.87 ± 0.85 and h = 288.37 ± 11.53 at 35 °C. The study demonstrates the potential of the approach and suggests further investigations into reproducibility and expanded color combinations. The results provide a promising basis for future improvements in temperature monitoring with thermochromic bubble sensors.

Surface enhanced infrared absorption mechanism and modification of the plasmonic response

Surface enhanced infrared absorption (SEIRA) is an experimental method where trace amount of a compound can be detected with high sensibility. This high detection sensibility is the result of the interaction of the molecules with a localized plasmon, usually from a metallic nanoparticle. In this study we numerically investigate by discrete dipole approximation the origin of the Fano-like response of the system, including the induced transparency when the plasmon resonance and the molecular vibrational mode coincide. The detailed analysis of the localization of the absorption shows that the modification of the absorption cross-section when the molecule is present comes from a change of the plasmonic resonance, not from the direct molecular response which is negligible. This sheds a new light on the SEIRA mechanism. In particular, it demonstrates that the sensibility is associated with the influence of the molecule on the plasmon resonance rather than with the local field enhancement itself.

Milk casein modification in response to dual oxidative enzymatic system

Milk casein modification in response to dual oxidative enzymatic system

Application of the Van Cittert Algorithm for Deconvolving Loss Features in X-ray Photoelectron Spectroscopy Spectra.

The convolution of two physical entities, denoted as f and g, delineates the manner in which one entity undergoes modification in response to the other. This transformative process is mathematically represented by the expression f ⨂ g, symbolizing the convolution of the two entities in a resultant function h. Frequently, it becomes imperative to comprehend the magnitude of the induced modifications. From the derived function h, a crucial step involves the separation of the two original signals, a process commonly referred to as deconvolution. Various techniques have been proposed to facilitate the calculation of the deconvolution, with one notable approach originating in 1931 by van Cittert. The algorithm, based on an iterative method, has been scrutinized over time, notably by Bracewell and, more recently, by Jansson. This work represents the current state-of-the-art, focusing specifically on the analysis of Auger spectra obtained through XPS. Emphasis is placed on delineating the procedural aspects of the analysis, and the algorithm utilized in the open-source software RxpsG is comprehensively described.

New insights into neuropathology and pathogenesis of autoimmune glial fibrillary acidic protein meningoencephalomyelitis.

Anti-glial fibrillary acidic protein (GFAP) meningoencephalomyelitis (autoimmune GFAP astrocytopathy) is a new autoimmune central nervous system (CNS) disease diagnosable by the presence of anti-GFAP autoantibodies in the cerebrospinal fluid and presents as meningoencephalomyelitis in the majority of patients. Only few neuropathological reports are available and little is known about the pathogenic mechanisms. We performed a histopathological study of two autopsies and nine CNS biopsies of patients with anti-GFAP autoantibodies and found predominantly a lymphocytic and in one autopsy case a granulomatous inflammatory phenotype. Inflammatory infiltrates were composed of B and T cells, including tissue-resident memory T cells. Although obvious astrocytic damage was absent in the GFAP-staining, we found cytotoxic T cell-mediated reactions reflected by the presence of CD8+/perforin+/granzyme A/B+ cells, polarized towards astrocytes. MHC-class-I was upregulated in reactive astrocytes of all biopsies and two autopsies but not in healthy controls. Importantly, we observed a prominent immunoreactivity of astrocytes with the complement factor C4d. Finally, we provided insight into an early phase of GFAP autoimmunity in an autopsy of a pug dog encephalitis that was characterized by marked meningoencephalitis with selective astrocytic damage with loss of GFAP and AQP4 in the lesions.Our histopathological findings indicate that a cytotoxic T cell-mediated immune reaction is present in GFAP autoimmunity. Complement C4d deposition on astrocytes could either represent the cause or consequence of astrocytic reactivity. Selective astrocytic damage is prominent in the early phase of GFAP autoimmunity in a canine autopsy case, but mild or absent in subacute and chronic stages in human disease, probably due to the high regeneration potential of astrocytes. The lymphocytic and granulomatous phenotypes might reflect different stages of lesion development or patient-specific modifications of the immune response. Future studies will be necessary to investigate possible implications of pathological subtypes for clinical disease course and therapeutic strategies.

Exploring the Association Between Behavioural Modification in Response to the Prevailing Economic Crisis and Mental Health Outcomes of Nurses from Teaching Hospitals, Sri Lanka.

The nursing profession is a cornerstone in the healthcare workforce. Yet, it remains vulnerable to severe mental health challenges, stemming from a complex interplay of social and occupational factors. It is evident that crisis situations have profound influence on the mental-wellbeing of nurses. To explore the association between behavioural modification in response to the prevailing economic crisis and mental health outcomes of nurses from teaching hospitals, Sri Lanka. This cross-sectional study was carried out among nursing professionals (n = 261) who are employed in distinct teaching hospitals (n = 7) in Sri Lanka. Considering the bed-capacity of each hospital, participants were purposively selected and invited to complete an online-based self-administered questionnaire to collect demographic characteristics and lifestyle modifications to deal with the economic crisis, while Depression, Anxiety, and Stress Scale (DASS-21) a set of three self-reported scales to assess the level of depression, anxiety, and stress, was used. Data was analyzed using the SPSS 23.0 version, Descriptive analysis was performed to present frequencies of both demographic characteristics and lifestyle and symptoms of depression, anxiety, and stress, while the Chi-square test was used to determine the association between selected independent variables and mental health outcomes. A multivariate regression model was performed to determine the predictors of mental health outcomes of the study participants. There were a total of 261 participants, 86.2% were married, and 66.2% had children. The proportion of mild to extreme depression, anxiety, and stress were 58.2%, 65.9%, and 72.0% respectively. In response to the economic challenges, 91.6% of study participants reported reducing expenditures on general household needs, while the proportion of participants who curtailed spending on food, monthly savings, and entertainment were 75.9%, 79.3%, and 73.6% respectively. Notably, 81.6% of participants initiated the process of applying for overseas employment, and 50.6% pursued academic programs to be eligible for foreign placements. The present study observed a higher level of depression, anxiety and stress among nurses from teaching hospitals in Sri Lanka. It was noted that nurses had taken steps to respond to financial strain by reducing essential elements of their routine. Additionally, study participants chose to seek foreign placements as a solution to the economic burden, as a result just over half of the respondents completed an academic program in order to be qualified for jobs abroad. Further, these initiatives are significantly associated with negative emotional disorders of the participants. Therefore, interventions should be implemented to promote positive mental health outcomes of nursing professionals in Sri Lanka.

Effect of Beam-Column Connection Types on the Response Modification Factors of Steel Frames

A key component of steel structural design is the careful selection of structural modeling joints in steel structures, as the behavior of the joints affects the structure's strength and displacement characteristics. According to the capacity for transferring moment, connections in the analysis of frames are categorized as rigid, semi-rigid, or pinned. Also, the response modification factor (R-factor) is an effective parameter used in the seismic design of structures. However, the influence of the beam-column connection's stiffness factor on the response modification factor did not seem to have been considered in seismic design codes. Consequently, the R-factor under static pushover and dynamic loading is being studied for moment-resisting steel frames (MRSFs) with 3-, 6-, and 12-story using three different forms of beam-column connections depending on the connections' stiffness (m). The rigidities of the connections are taken 20, 10, and 5 for rigid, stiff semi-rigid, and flexible semi-rigid connections, respectively. The MRSFs are subjected to ten records with varying frequency contents and ground motion durations. The ductility reduction factor (Rμ), the over-strength reduction factor (Ro), and the R-factor were determined. The results indicated that the beam-column connection rigidity factors affected the R0, Rμ, and R-factors. Also, the R-factors were more affected by the rigidity factors for the beam-column connections and the number of story frames.