Mode Of Sterilization Research Articles

New determining method the mode of sterilization of canned food

New determining method the mode of sterilization of canned food

Fabrication of a superhydrophobic cotton fabric with efficient antibacterial properties and asymmetric wettability via synergistic effect of quaternized chitosan/TiO2/Ag

A superhydrophobic cotton fabric with unidirectional moisture transfer ability, antibacterial, and oil-water separation capabilities was fabricated by combining a simple coating method and oxygen plasma etching. Polydimethylsiloxane (PDMS) was employed to impart low surface energy to the cotton fabric, thereby endowing it with hydrophobic characteristics. The synthesized quaternized chitosan/TiO2/Ag (CTA) compound was subsequently applied onto the cotton fabric surface to introduce a desirable level of surface roughness, thus enhancing its hydrophobic properties and conferring excellent antibacterial efficacy. The cotton was subsequently treated by oxygen plasma to trigger asymmetrical wetting on both sides. The experimental findings reveal that the optimally treated cotton fabric exhibits hydrophobic properties on the bottom surface, with a water contact angle (WCA) reaching 162.8°, while the upper surface displays hydrophilic behavior, establishing a wetting gradient along the thickness direction. Notably, even under the influence of reverse gravity, water droplets are capable of transferring from the back to the top surface within 2 s, while preventing reverse penetration of liquid. Moreover, the synthesized compound CTA endows the cotton fabric with efficient and long-lasting antibacterial properties through two modes (contact and release sterilization modes) and three sterilization mechanisms (electrostatic interaction, plasma surface resonance effect, and reactive oxygen species). Remarkably, within a time frame of only 1 h, the modified cotton fabric is able to achieve a kill rate exceeding 99% against Escherichia coli and Staphylococcus aureus. Moreover, the aforementioned properties demonstrate outstanding durability against repeated washing, continuous abrasion, exposure to strong acid/base, and ultraviolet radiation. Additionally, this modified cotton fabric exhibits remarkable efficiency in terms of oil-water separation, effectively separating both light and heavy oil from water. It is worth noting that the preparation process is cost-effective, environmentally friendly, and free from fluorine.

METHODS OF STERILIZATION OF MEDICAL POLYMER COMPOSITES BASED ON POLYHYDROXYBUTYRATE WITH ELASTOMERIC ADDITIVE

Introduction. Polyhydroxybutyrate is a biodegradable and completely biocompatible component, and in combination with various modifying additives can be suitable for the manufacture of medical products used in surgical practice as bone implants or their parts. There implants have a number of advantages over traditional metal products, but for their integration into the body they require thorough sterilization cleaning, which in the case of poly-mer compositions has a number of limitations associated with the possible destruction of the material structure during various cleaning stages. Purpose of the study. Find optimal methods for sterilization and disinfection of materials based on polyhydroxybutyrate (PHB) and an elastomeric additive – butadiene-nitrile rubber (NBR-28). Material and methods. Two-component PHB-NBR compositions with PHB content from 30 to 90% were studied. Four methods of sterilization and disinfection were used: autoclaving, air sterilization, disinfection with chlorine solution and ethanol solution. Determined mechanical characteristics are strength and elongation of the material at break. Sterility control – by the method of washings with subsequent observation of the growth of bacteria and fungi in Sabouraud's medium and thioglycollate medium. Results. Sterilized and disinfected samples showed no microbial growth in both culture media. No change in mechanical characteristics was detected for samples subjected to solution cleaning methods. High temperature cleaning reduced the mechanical properties of samples by 20–80% depending on the sterilization mode. Conclusions. The data obtained show that for sterilization and disinfection of PCM based on the biodegradable polymer PHB, solution methods are suitable without restrictions and the autoclave sterilization method is suitable with minor restrictions, while air sterilization leads to the destruction of PCM. The work was financially supported by the Ministry of Science and Higher Education of Russian Federation (Research theme state registration number 122041300207-2).

Оптимизация условий микроклонального размножения и среднесрочного хранения in vitro редкого вида смородины Янчевского для сохранения

В статье представлены результаты исследований по сохранению редкого вида растения смородины Янчевского (Ribes janczewskii) в условиях in vitro. Этот вид смородины более устойчив к болезням, вредителям и низким температурам, а также содержит большое количество полезных веществ, таких как аскорбиновая кислота, полифенолы и антоцианы по сравнению с другими видами. Ранее не проводились исследования по разработке биотехнологии для сохранения этого исчезающего вида. Авторами данной работы подобраны эффективная стерилизация и введение эксплантов смородины Янчевского в культуру in vitro, в качестве стерилизующего агента выбран 12 %-ный раствор перекиси водорода с режимом стерилизации 5 мин, где жизнеспособность эксплантов достигла до 73,3 %. Также для мультипликации оптимизирован состав питательной среды WPM с добавлением БАП 0,5 мг/л, ГК 0,4 мг/л и ИМК 0,1 мг/л, который позволил получить 4,83 новых побега на эксплант. Для среднесрочного хранения микропобегов смородины Янчевского наиболее оптимальным условием была выбрана питательная среда WPM с добавлением маннита в концентрации 20 гр/л, в результате чего прирост по высоте побегов и количеству листьев был низким, размножение побегов не наблюдалось. Была создана коллекция смородины Янчевского в культуре in vitro, которая позволит размножить и сохранить этот ценный вид. В настоящее время микроклонально размножено 250 побегов, из которых 120 побегов были культивированы на питательных средах для среднесрочного хранения.

Short-chain fatty acids levels in human milk are not affected by holder pasteurization and high hydrostatic pressure processing.

Sterilized donor milk (DM) is frequently used for feeding preterm infants. To date, the effect of different modes of DM sterilization on short-chain fatty acids (SCFAs) remains unknown. We aimed to quantify SCFAs in DM samples after two types of milk sterilization: the Holder pasteurization (HoP) and a high hydrostatic pressure (HP) processing. Eight pooled DM samples were sterilized by HoP (62.5°C for 30 min) or processed by HP (350 MPa at 38°C). Raw DM was used as control. Six SCFAs were quantified by gas chromatography/mass spectrometry. Compared to raw milk, both HoP and HP treatment did not significantly modulate the concentration of acetate, butyrate, propionate and isovalerate in DM. Valerate and isobutyrate were undetectable in DM samples. In conclusion, both HoP and HP processing preserved milk SCFAs at their initial levels in raw human milk.

Albumin redox state of maintenance haemodialysis patients is positively altered after treatment

Background and AimMaintenance haemodialysis patients have increased morbidity and mortality which is mainly driven by an elevated inflammation level due to the uraemic milieu. A major part of this increased inflammation level is the degree of oxidative stress which can be assessed by albumin redox state (ARS). Aim of this study was to evaluate how the ARS is affected by a haemodialysis treatment and different dialyzer properties.MethodsARS was determined before and after haemodialysis treatment by fractionating it into reduced human mercaptalbumin (HMA), reversibly oxidized human non-mercaptalbumin 1 (HNA-1), and irreversibly oxidized human non-mercaptalbumin 2 (HNA-2) by high-performance liquid chromatography. In healthy individuals, albumin circulates in the following proportions: HMA 70–80%, HNA-1 20–30% and HNA-2 2–5%. High flux (FX 100 [Fresenius Medical Care], BG 1.8 [Toray], Xevonta Hi 18 [B. Braun], CTA-2000 [Kawasumi]) and low flux FX10 [Fresenius Medical Care] dialyzers were used.Results58 patients (59% male, median age 68 years, median time on haemodialysis 32 month) were included in the study. Before haemodialysis treatment, HMA (median 55.9%, IQR 50.1–61.2%) was substantially lower than in healthy individuals. Accordingly, oxidized albumin fractions were above the level of healthy individuals (median HNA-1 38.5%, IQR 33.3–43.2%; median HNA-2 5.8%, IQR 5.1–6.7%). Before haemodialysis treatment HMA was significantly higher in patients usually treated with high flux membranes (p < 0.01). After haemodialysis treatment there was a significant increase of HMA and a decrease of HNA-1 and HNA-2 (p < 0.01). These effects were more pronounced in patients treated with high flux dialyzers (p < 0.01). There were no differences of ARS alteration with regard to the dialyzer´s sterilization mode or the presence of diabetes.ConclusionThe study confirms that the ARS is positively altered by haemodialysis and shows for the first time that this effect depends on dialyzer properties.

Effect of gamma and Ultraviolet-C sterilization on BMP-7 level of indigenously prepared demineralized freeze-dried bone allograft.

The presence of bone morphogenetic proteins in demineralized freeze-dried bone allograft (DFDBA) are responsible for developing hard tissues in intraosseous defects. The most common mode of sterilization of bone allografts, i.e., Gamma rays, have dramatic effects on the structural and biological properties of DFDBA, leading to loss of BMPs. Ultraviolet-C radiation is a newer approach to sterilize biodegradable scaffolds, which is simple to use and ensures efficient sterilization.However, UV-C radiation has not yet been effectively studied to sterilize bone allografts. This study aimed to compare and evaluate the effectiveness of Gamma and Ultraviolet-C rays in sterilizing indigenously prepared DFDBA and assess their effect on the quantity of BMP-7 present in the allograft. DFDBA samples from non-irradiated, gamma irradiated, and UV-C irradiated groups were tested for BMP-7 level and samples sterilized with gamma and UV-C rays were analysed for sterility testing.The estimated mean BMP-7 level was highest in non-irradiated DFDBA samples, followed by UV-C irradiated, and the lowest in gamma irradiated samples. Our study concluded that UV-C rays effectively sterilized DFDBA as indicated by negative sterility test and comprised lesser degradation of BMP-7 than gamma irradiation.

Knowledge, attitude and practice about menstrual cup among healthcare professionals

Aim: To assess the knowledge, attitude and practice about menstrual cup among health care professionals. Methods: A cross-sectional study was done using structured questionnaire method to collect data among health care professionals in Mangalore. Total sample size of 330 was taken. Results: Out of total 330 participants, 296 (91%) were aware of the menstrual cup and media was their main source of information. Maximum number of participants had a good knowledge about menstrual cups in terms of its various sizes, durability, mechanism of action, material used and mode of sterilization, cost of the cup. 87.6% participants are using sanitary pads currently. Discomfort (54.2%) was the major concern regarding menstrual cup use. A menstruation cup would be used by 137 (42.4%) of the participants if it was made available. Conclusion: Despite of good knowledge about the menstrual cup amongst the health care professionals in the study, practice was found to be poor. Healthcare professionals, who are more open to the idea of eco-friendly products, should be addressed in order to increase the adoption rate of menstruation cups. The government must implement an awareness campaign so that the dream of a

Knowledge, attitude and practice about menstrual cup among healthcare professionals

Aim: To assess the knowledge, attitude and practice about menstrual cup among health care professionals. Methods: A cross-sectional study was done using structured questionnaire method to collect data among health care professionals in Mangalore. Total sample size of 330 was taken. Results: Out of total 330 participants, 296 (91%) were aware of the menstrual cup and media was their main source of information. Maximum number of participants had a good knowledge about menstrual cups in terms of its various sizes, durability, mechanism of action, material used and mode of sterilization, cost of the cup. 87.6% participants are using sanitary pads currently. Discomfort (54.2%) was the major concern regarding menstrual cup use. A menstruation cup would be used by 137 (42.4%) of the participants if it was made available. Conclusion: Despite of good knowledge about the menstrual cup amongst the health care professionals in the study, practice was found to be poor. Healthcare professionals, who are more open to the idea of eco-friendly products, should be addressed in order to increase the adoption rate of menstruation cups. The government must implement an awareness campaign so that the dream of a

Use of consumer polymer С-РET containers in food production technologies

Different types of consumer containers are used for food packaging: glass, metal, polymer. Polymer containers are in the greatest demand among consumers, due to such advantages as cheapness, small weight, unlimited range of products and volume, pleasant, bright appearance. In the food industry, hard, semi-hard, soft and other consumer polymer containers are used. To use such packaging in technology with high-temperature heat treatment of food products, it must have a heat-resistant barrier layer. Therefore, the object of research is a polymer combined C-PET container, which consists of a semi-rigid container-tray and a heat-resistant multi-layer polymer soft film for its closure. C-PET packaging is made of barrier polymer materials that ensure its mechanical resistance to high temperatures. Therefore, such containers can withstand high-temperature processing and guarantee the tightness of the package and the microbiological stability of the product during storage. Each polymer material has its own specific indicators of heat resistance. The work solves the problem of using the latest polymeric C-PET containers for long-term storage food products, investigates the conditions for preserving the tightness of the containers during heat treatment, which are ensured by the clogging strength parameter. Different types of polymer films for sealing C-PET packaging with the product are also investigated and their mechanical characteristics are compared. In the course of the study, a standard membrane-compensation method was used to measure the clogging strength or depressurization pressure of the package. The essence of the obtained results: the parameters of the use of different types of polymer films of different types were experimentally determined, on the basis of which the type of film was chosen, which ensures the clogging strength of C-PET containers. The results are explained by the fact that the depressurization pressure value obtained will allow to develop scientifically based thermal modes of sterilization and pasteurization for food products in C-PET containers. This will make it possible in practice for enterprises to apply the same regimes and produce high-quality, biologically stable, safe food products with a long shelf life.

Obtaining sterile Calligonum aphyllum material for in vitro culture preservation

The comparative evaluation’s results on the efficiency of surface treatment of Calligonum aphyllum (Pall.) Guerke explants, a perspective plant species in the restoration of degraded lands, are presented. Sterilizers «Lysoformin 3000», silver nitrate, hydrogen peroxide, and «Belizna-econom» are used at the introduction stage of the plants in vitro. As a result, 2 of 11 effective sterilization modes were found when C. aphyllum was introduced into the culture in vitro: No. 10 (hydrogen peroxide solution 10% v/v, 5 min) and No. 11 (hydrogen peroxide solution 6% v/v, 15 min). These modes had the highest rates of sterile and regenerative explants. The microflora was also isolated from the studied explant samples. Determined the preparation «Lysoformin 3000» effectively acts on all isolated microorganisms. «Belizna-econom» and hydrogen peroxide (concentrations of 10% v/v) do not act on 80% of isolated bacterial strains. Shown the silver nitrate solution was not sensitive in 100% of cases. The obtained information can be used to draw up a complete protocol for C. aphyllum explant sterilization at the introduction stage in vitro.

Особенности введения в культуру in vitro барбариса илийского (Berberis iliensis M. Pop.)

The results of the introduction of Berberis iliensis M. Pop., a rare endemic plant, into in vitro culture are presented. The rare species is currently endangered. Methods of introduction into in vitro culture have been worked out, sterilizing agents and sterilization modes have been selected (5% bleach solution “Belizna”, treatment time 5 min, 10% hydrogen peroxide solution, exposure time 7 min). The WPM nutrient medium, with a pH level of 5.6–5.8, was selected as optimal for the successful development of Berberis iliensis plants in in vitro culture. Vitamins were added to the standard mineral composition of the medium. Magentas with explants were cultivated in a room without access to light at a temperature of 22–25°C and a humidity of 70% for 3–4 weeks. Further cultivation was carried out at 26°C, a light period of 16 h, and illumination of 4000–5000 lux. Aseptic cultures of B. iliensis were obtained and they had a regenerative capacity. Shoots were formed without callus, that is, direct regeneration is observed, which is the most favorable for micropropagation.

Efficiency of accelerated electron beam sterilization of a hydrogel for 3D cultivation of mesenchymal multipotent cells

Background. Hydrogels are promising for use in tissue engineering for the restoration and regeneration of various tissues, since they are able to perform the functions of bulk scaffolds, providing the formation of 3D cell structures. Population of such scaffolds with autologous or heterogeneous mesenchymal multipotent stromal cells in vitro makes it possible to localize these cells in the area of target tissues after implantation in a patient. One of the difficult tasks is the choice of the method and mode of sterilization of the hydrogel, which does not change its properties.Aim. Study of the effectiveness of hydrogel sterilization by an accelerated electron beam in various modes, changes in the structure and biocompatibility of the scaffold, to assess the prospects for its use for medical purposes, including as a platform for mesenchymal stromal cells.Materials and methods. We used a hydrogel based on 4 % solutions of sodium alginate and sodium salt of carboxymethyl cellulose, cross-linked with calcium chloride, which was developed, obtained and provided for our research by the team of the Research and Educational Center for Biomedical Engineering of the National University of Science and Technology “MISIS”. Hydrogel samples loaded with Escherichia coli, Lactobacillus acidophilus, Saccharomyces cerevisiae were subjected to electron beam treatment in the range of 5–100 kGy. After electron beam treatment of hydrogel, the presence of living microorganisms and its structure were evaluated by IR-Fourier spectroscopy, as well as the phenotype and formation of 3D structures by mesenchymal multipotent cells.Results. It was found that the treatment of hydrogels with an electron beam at a mode of 25 kGy ensures the death of microorganisms, but does not destroy the structure of the hydrogel and does not inhibit the ability to form capillary-like structures by mesenchymal multipotent cells.Conclusion. Treatment with an accelerated electron beam at a 25 kGy can be used to sterilize hydrogels to obtain bulk scaffolds for cell engineering implants.

Ultra-fast bacterial inactivation of Cu2O@halloysite nanotubes hybrids with charge adsorption and physical piercing ability for medical protective fabrics

• Rapid inactivation efficiency, security nano-Cu2O hybrid antibacterial material. • The effective antibacterial mechanism of Cu2O@HNTs. • Realization of high efficiency and broad-spectrum antibacterial protective fabric. Metals have been used for wound treatment and toxicity testing since ancient times. With the development of nanotechnology, metal oxides have been proven to have excellent sterilization and disinfection functions. However, the rapid bacterial inactivation efficiency and trapping physicochemical killing ability remain simultaneously undemonstrated in antibacterial nanohybrids. Here, we demonstrate a method for in-situ reduction of small-sized Cu 2 O particles on one-dimensional inorganic halloysite nanotubes (HNTs). The resultant Cu 2 O@HNTs hybrids not only give Cu 2 O excellent dispersibility, but also exert the synergistic effect of the charge adsorption of metal oxides and the physical piercing effect of the small-sized nanotubes. Furthermore, the release of Cu 2+ from hybrids damages cell membranes and denatures proteins and DNA. Through this sterilization mechanism, Cu 2 O@HNTs allow for the inactivation rate of Escherichia coli to reach 94.5% within 2 min and complete inactivation within 10 min. This excellent sterilization mode makes Cu 2 O@HNTs exhibit excellent broad-spectrum antibacterial activity and inactivation efficiency, while shows weak cytotoxicity. These hybrids were further applied in the processing of functional antibacterial fibers and fabrics. Thus, we believe that this excellent antibacterial hybrid is practically attractive in this critical time of the COVID-19 pandemic.

Efficient visible light-harvesting film with multi-channel sterilization behavior for ultra-persistent freshness of perishable products

In light of the microbial spoilage of perishable products such as fruits and vegetables, this work pioneered a visible light-harvesting film with multi-channel sterilization mode by the demand-oriented construction of functional fillers, aiming to efficiently utilize abundant sunlight to achieve long-term preservation of fruits and vegetables. In detail, cubic TiO2-anchored porous nitrogen-rich nanocarbons (TPNC) with excellent photothermal and photocatalytic activities was first prepared by two-step annealing of NH2-MIL-125(Ti) precursor in different atmospheres, which was incorporated as filler into chitosan (CS) matrix to obtain a series of TPNC-CS nanocomposite films. Benefiting from the synergy between the integrated photothermal and photocatalytic sterilization of TPNC and the inherent antibacterial properties of CS, the TPNC0.30-CS film exhibited outstanding antibacterial capacity with short processing times (10 min) and high antibacterial rates (≥99.4 %). The significantly prolonged shelf life of kumquats treated with TPNC0.30-CS film confirmed its satisfactory preservation ability. Although at the cost of a slight loss of transparency, upgraded performances in mechanical strength, barrier capability, and proven biosafety served as cornerstones for the practical application of TPNC-CS films. These desirable properties declared TPNC-CS films as a promising microbial control barrier for efficient preservation of fruits and vegetables.

Planning Implications Related to Sterilization-Sensitive Science Investigations Associated with Mars Sample Return (MSR).

Planning Implications Related to Sterilization-Sensitive Science Investigations Associated with Mars Sample Return (MSR).

USE OF POLYMER PACKAGING FOR VARIOUS FOOD PRODUCTS WITH LONG-TERM STORAGE

In the food industry, for thermal preservation of food products, various types of consumer polymer packaging can be used: rigid, semi-rigid, soft, and others. In order for plastic (polymer) packaging to be used for heat sterilization and guarantee long shelf life of food products, it must contain the necessary heat-resistant barrier layer, which will ensure the resistance of the container to high temperatures. Each polymeric material has certain indicators of heat resistance. In the work, we used such types of consumer packaging for food packaging, such as a combined metal can with a polymer lid, С-PET containers. The purpose of the study is to develop the conditions for canning, sterilization modes of food products in the range for new types of consumer polymer for specific thermal equipment. When using various types of containers for thermal sterilization and prevention of physical defects of canned food, it is necessary to take into account such technological features as the sealing strength or depressurization pressure, the sealing method, the presence or absence of a stiffness relief on the lid, the product packaging temperature and other factors. The standard membrane-compensation method was used to measure the sealing strength or the pressure of depressurization of the container, which occurs during sterilization due to thermal expansion of the product. The development of sterilization modes for canned food was carried out in accordance with the approved regulations, which includes an analytical calculation of the mode that ensures the production of industrially sterile canned food, laboratory testing of the selected mode and its production verification. The mode calculation method is based on the data on the heat resistance of the microorganism strain of the test culture, which should guarantee the industrial sterility of canned food. In the work, studies were carried out, as a result of which data were obtained on an important technological parameter of the packaging strength of sealing, without the values of which it is impossible to carry out high-quality thermal sterilization of the product. Scientifically based parameters, hightemperature sterilization regimes for meat, fish, and vegetable products in modern polymer types of consumer packaging have also been developed, which will allow enterprises to produce canned high-quality food products that are safe to use, with high nutritional value.

Optimized UV-Spectrophotometric Assay to Screen Bacterial Uricase Activity Using Whole Cell Suspension.

Uricase catalyzes the conversion of uric acid into allantoin with concomitant reduction of molecular oxygen to hydrogen peroxide. In humans, uricase is not functional, thereby predisposing individuals to hyperuricemia, a metabolic disturbance associated with gout, chronic kidney disorders, and cardiovascular diseases. The efficacy of current therapies to treat hyperuricemia is limited, and novel approaches are therefore desired, for instance using uricase-expressing probiotic strains. Here, we evaluated UV-spectrophotometric and H2O2-based fluorescent assays to enable the rapid identification of uricase activity in a broad panel of lactobacilli, Bacillus, and Bifidobacterium species. We highlighted abiotic (medium composition and mode of sterilization) and biotic (H2O2-producing strains) factors impacting the measurements’ accuracy, and reported on the stepwise optimization of a simple, fast, and robust high-throughput UV-spectrophotometric method to screen uricase activity using whole bacterial suspension, thereby assessing both cell-associated and extracellular activity. The validity of the optimized assay, based on the monitoring of uric acid degradation at 300 nm, was confirmed via liquid chromatography. Finally, a panel of 319 Qualified Presumption of Safety (QPS) strains of lactobacilli (18 species covering nine genera), Bacillus (three species), and Bifidobacterium (four species) were screened for uricase activity using the optimized method. All 319 strains, but the positive control Bacillus sp. DSM 1306, were uricase-negative, indicating that this activity is rare among these genera, especially in isolates from food or feces. Altogether, the UV-spectrophotometric high-throughput assay based on whole bacterial suspension reported here can be used to rapidly screen large microbial collections, by simultaneously detecting cell-associated and extracellular uricase activity, thereby accelerating the identification of uricolytic strains with therapeutic potential to treat hyperuricemia.

Optimization of berry crop genotype regeneration systems at the in vitro crop initiation stage

The article presents data obtained during the study of the effectiveness of the initial stage of in vitro introduction of berry crops into culture: black currant (Ribes nigrum L.) and pine strawberry (Fragaria × ananassa Duch.) depending on the period of implementation and sterilizing agents used. The objects of research are varieties of black currant (Azhurnaya, Orlovskaya Serenada, Ocharovanie, Chudnoye Mgnovenye); pine strawberry (Asia, Alba, Bereginya, Darselekt, Kimberly, Klery, Marmolada Onebor, Siria, Honeoye, Florence, Frida, Tsaritsa, Urozhajnaya CGL). Introduction into culture in vitro was carried out in several periods characterized by different physiological state of explants: dormancy release, active growth, and retardation. Cultivation was carried out on Murashige-Skoog medium with the addition of 6-BAP (0.5 mg/l). It is noted that the survival rate of berry crop explants is determined by the physiological state of the source material, due to the period of introduction, and the genotypic characteristics of the varieties. The study of various sterilization modes of the initial plant material showed the effectiveness of such sterilizing agents as hydrogen peroxide (12.0%), merthiolate (0.01%), mercury bichloride (sulema, 0.1%), argentic nitrate (0.2%).

Development of technology for production of functional fruit drinks

The object of research is the technology of functional fruit and vegetable juice products enriched with collagen. The subject of research is various types of collagen, formulations and parameters of technological operations for the production of functional juice drinks. The research is aimed at the develop technology, recipes for a new assortment of fruit and vegetable drinks enriched with collagen. Also it is aimed at the preserve the functional properties of collagen in canned juice products after high-temperature processing and create food products for everyday consumption to solve age-related and other problems associated with human health. In the course of the study, standard methods were used to determine the organoleptic indicators of various types of collagen, juice products, as well as to determine the mass fraction of moisture, carbohydrates, fats, proteins in the developed fruit and vegetable drinks enriched with collagen. Also, to confirm the presence and preservation of collagen in finished products, the electrophoresis method was used to determine the atomic mass unit of the constituent substances after complete drying of the samples. The proposed methods make it possible to assess the quality of the developed collagen-enriched drinks, prove the presence and preservation of a biologically active additive in the finished product after using the sterilization mode parameters – temperature and time, when receiving canned products. The developed formulations and technology make it possible to consider functional fruit and vegetable juice-containing products enriched with a biologically active additive collagen as effective and useful. An easily digestible food product for everyday consumption is proposed for solving age-related and other problems related to human health, with regulation of the pH value for effective use of the beneficial properties of this additive. In contrast to existing functional juice products, the proposed formulations and technology make it possible to obtain juice-containing fruit and vegetable blended products with a biologically active additive collagen. The technology minimizes the impact of collagen on the organoleptic characteristics of the developed recipe compositions of finished products and preserves its beneficial biologically active properties in the finished canned product after heat sterilization.