PURPOSE: To examine children’s moderate-to-vigorous physical activity (MVPA), light physical activity (LPA), and sedentary behavior (SB) during morning and lunchtime recess and after-school on different school days (physical education [PE] day vs. exergaming day) and across different weight status. METHODS: One-hundred forty 2nd and 3rd graders (X[Combining Overline]age = 8.14 years) from a public school took part in the study. Children engaged in 125 minutes of structured weekly PA, with 25-min. exergaming alternating PE daily. Children also had a 20-min. morning recess and a 30-minute lunch recess daily. Fourteen hours (8:00am-10:00pm) were designated as children’s school day time, with 400 minutes (3:20–10:00pm) classified as the after-school segment. Data was collected for 3 PE days and 3 exergaming days. Actigraph accelerometers were used to track MVPA, LPA, and SB during all school and after-school activities. Further, students were classified into three weight groups according to CDC BMI Growth charts: healthy; risk of overweight; and overweight. RESULTS: A significant main effect for day (PE or exergaming) (Wilk’s λ=0.58; p<0.01) but not weight (Wilk’s λ=0.93; p=0.15) was seen in regards to PA levels. Specifically, LPA was found to be higher during exergaming classes while MVPA was higher during PE classes. Regarding PA differences during morning recess and lunchtime recess, significant main effects were observed for day (Wilk’s λ=0.93; p=0.02) and weight (Wilk’s λ=0.90; p=0.03) during morning recess; and a significant main effect was seen for day during lunchtime recess (Wilk’s λ=0.94; p=0.04). Finally, a significant main effect for weight (Wilk’s λ=0.87; p=0.004) but not day (Wilk’s λ=0.97; p=0.19) was observed for children’s after-school PA. Engagement in LPA and MVPA was generally higher during both recess periods and after-school segment on exergaming days. CONCLUSIONS: Findings revealed a general trend wherein healthy and risk of overweight children engaged in more LPA and MVPA and less SB than overweight children during recess and after-school. Moreover, exergaming days appeared to stimulate greater amounts of LPA and MVPA during recess and after-school compared to PE days. Future PA interventions may concentrate the greatest amount of attention on overweight children-perhaps utilizing exergaming to do so.