Moderate-intensity Endurance Training Research Articles

Moderate-intensity endurance training has higher effects suppression of oxidative stress secretion than strength training in obese students

Moderate-intensity endurance training has higher effects suppression of oxidative stress secretion than strength training in obese students

Therapeutic effects of the combination of moderate-intensity endurance training and MitoQ supplementation in rats with isoproterenol-induced myocardial injury: The role of mitochondrial fusion, fission, and mitophagy

IntroductionMitochondrial dysfunction causes myocardial disease. This study investigated the effects of MitoQ alone and in combination with moderate-intensity endurance training (EX) on cardiac function and content and mRNA expression of several proteins involved in mitochondrial quality control in isoproterenol (ISO)-induced heart injuries MethodsSeven groups of CTL, ISO, ISO-EX, ISO-MitoQ-125, ISO-MitoQ-250, ISO-EX+MitoQ-125, and ISO-EX+MitoQ-250 were assigned. Rats were trained on a treadmill, and the MitoQ groups received MitoQ in drinking water for 8 weeks, starting one week after the induction of heart injury. Arterial pressure and cardiac function indices, mRNA expression, protein content, oxidant and antioxidant markers, fibrosis, and histopathological changes were assessed by physiograph, Real-Time PCR, immunofluorescence, calorimetry, Masson’s trichrome, and H&E staining, respectively. ResultsThe impacts of MitoQ-125, EX+MitoQ-125, and EX+MitoQ-250 on arterial pressure and left ventricular systolic pressure were higher than MitoQ-250 or EX alone. ± dp/dt max were higher in ISO-EX+MitoQ-125 and ISO-EX+MitoQ-250 than ISO-MitoQ-125 and ISO-MitoQ-250 groups, respectively. Histopathological scores and fibrosis decreased in ISO-EX, ISO-MitoQ-125, ISO-EX+MitoQ-125, and ISO-EX+MitoQ-250 groups. The restoration of MFN2, PINK-1, and FIS-1 changes was higher in ISO-EX+MitoQ-125 and ISO-EX+MitoQ-250 than ISO-EX, ISO-MitoQ-125 and ISO-MitoQ-250 groups. The expression of MFN2 and PINK-1 was lower in ISO-MitoQ-125 and ISO-EX+MitoQ-125 than ISO and CTL groups. The expression of FIS-1 in ISO-EX and ISO-EX+MitoQ-250 increased compared to CTL and ISO groups. MDA decreased in ISO-MitoQ-125 and ISO-EX+MitoQ-125 groups. ConclusionExercise and MitoQ combination have additive effects on cardiac function by modulating cardiac mitochondria quality. This study provided a possible therapy to treat heart injuries.

Exercise-related alterations in MCT1 and GLUT4 expressions in the liver and pancreas of rats with STZ-induced diabetes.

The liver and pancreas tissues play a central role in controlling glucose homeostasis. In patients with type I diabetes mellitus (T1DM), the function of these tissues is impaired. The positive effects of exercise have been shown in diabetic patients. To demonstrate the positive effects of exercise in T1DM, we examined the effects of moderate-intensity endurance training (MIET) on the liver enzymes and expression of MCT1 and GLUT4 genes. Male Wistar rats were allocated into 4 groups of control (C), training (T), diabetic control (DC), and diabetes + training (DT). The serum levels of liver enzymes such as alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) were determined by ELIZA. MCT1 and GLUT4 mRNA expressions in the liver and pancreas tissues were evaluated through real-time qPCR after 10weeks of training. The mRNA levels of MCT1 and GLUT4 decreased in DC group and increased in DT group. T1DM led to weight loss, but the weight loss was less in the DT group. T1DM caused an increase in liver enzymes such as ALT, AST and ALP, whereas endurance training preserved enzymatic levels. These results suggested that MIET increases levels of MCT1 and GLUT4 liver and pancreas in the diabetic rats and improves liver function tests. Upregulation of MCT1 and GLUT4 can probably improve the function of liver and pancreas tissues and promote glucose homeostasis in T1DM.

Moderate-intensity endurance training improves late phase β-cell function in adults with type 2 diabetes

Physical activity is important for type 2 diabetes treatment, yet the underlying mechanisms for these beneficial effects of exercise are not fully understood. Here, we investigated the effects of exercise training on biphasic β-cell insulin secretory function, a key factor regulating blood glucose. Adults with type 2 diabetes (7F/3M, age 49± 5 years, BMI 30± 3kg/m2) completed a 10-week moderate-intensity exercise program and multiple components of glucose homeostasis were measured. Training improved glycemic control, insulin sensitivity, and processing of proinsulin-to-insulin. Training increased late phase β-cell function by 38% (p= 0.01), which was correlated with changes in VO2peak suggesting training response-dependent effects. Ras-Responsive Element Binding Protein 1 (RREB1) concentrations, a protein postulated to increase type 2 diabetes risk, were inversely correlated with increases in training-induced late-phase disposition index, consistent with an inhibitory role of RREB1 on insulin secretion. Moderate-intensity exercise training improves late-phase β-cell function and glycemic control in adults with type 2 diabetes.

The effect of eight weeks of moderate-intensity endurance training on serum levels of troponin I and B-type natriuretic peptide in radiotherapy rats

Background and aims: One of the most important potential problems of radiotherapy is the heart problem caused by this treatment. Therefore, this research aimed to investigate the effect of 8 weeks of moderate-intensity endurance training on the serum levels of troponin I (TNI) and brain (B-type) natriuretic peptide (BNP) in rats undergoing radiation therapy. ] Methods: In this experimental study, 32 male rats (4-6 months) were randomly divided into four groups of eight, including healthy control (C), aerobic training (AT), radiotherapy (RT), and AT+RT groups. First, rats were anesthetized with ketamine-xylazine solution (K: 60-90 kg/mg, Z: 6-10 kg/mg) and then located on a Plexiglas plate with a thickness of 1 cm. Photon beam RT was performed using X-rays with a dose of 11 Gy from an Elekta compact linear accelerator (Elekta Compact 6-MV China). AT program was performed for eight weeks, five days a week, and one session a day for 60 minutes (70-75% of maximal oxygen consumption). Finally, one-way ANOVA was run to examine the research variables. Results: The results showed that there was no significant difference between the groups in terms of the TNI level (P=0.23). However, a significant difference was found in the amount of BNP between the RT and C groups (P=0.009). In addition, no significant difference was reported in terms of BNP between AT+RT with AT (P=0.99), RT (P=0.32), and C (P=0.69) groups, as well as between AT with RT (P=0.1) and C (P=0.99) groups. Conclusion: Overall, radiation therapy caused a significant increase in BNP, but it had no significant effect on TNI. Aerobic training did not significantly affect TNI and BNP in healthy rats and those undergoing radiation therapy.

Does Higher Intensity Increase the Rate of Responders to Endurance Training When Total Energy Expenditure Remains Constant? A Randomized Controlled Trial

BackgroundStandardized training prescriptions often result in large variation in training response with a substantial number of individuals that show little or no response at all. The present study examined whether the response in markers of cardiorespiratory fitness (CRF) to moderate intensity endurance training can be elevated by an increase in training intensity.MethodsThirty-one healthy, untrained participants (46 ± 8 years, BMI 25.4 ± 3.3 kg m−2 and {dot{text{V}}}O2max 34 ± 4 mL min−1 kg−1) trained for 10 weeks with moderate intensity (3 day week−1 for 50 min per session at 55% HRreserve). Hereafter, the allocation into two groups was performed by stratified randomization for age, gender and VO2max response. CON (continuous moderate intensity) trained for another 16 weeks at moderate intensity, INC (increased intensity) trained energy-equivalent for 8 weeks at 70% HRreserve and then performed high-intensity interval training (4 × 4) for another 8 weeks. Responders were identified as participants with VO2max increase above the technical measurement error.ResultsThere was a significant difference in {dot{text{V}}}O2max response between INC (3.4 ± 2.7 mL kg−1 min−1) and CON (0.4 ± 2.9 mL kg−1 min−1) after 26 weeks of training (P = 0.020). After 10 weeks of moderate training, in total 16 of 31 participants were classified as VO2max responders (52%). After another 16 weeks continuous moderate intensity training, no further increase of responders was observed in CON. In contrast, the energy equivalent training with increasing training intensity in INC significantly (P = 0.031) increased the number of responders to 13 of 15 (87%). The energy equivalent higher training intensities increased the rate of responders more effectively than continued moderate training intensities (P = 0.012).ConclusionHigh-intensity interval training increases the rate of response in VO2max to endurance training even when the total energy expenditure is held constant. Maintaining moderate endurance training intensities might not be the best choice to optimize training gains.Trial Registration German Clinical Trials Register, DRKS00031445, Registered 08 March 2023—Retrospectively registered, https://www.drks.de/DRKS00031445

The effect of moderate-intensity endurance plus resistance training combined with MCCR diet on glycemic status in prediabetes

BackgroundThere is little clinical evidence that exercise improves prediabetic individuals' glycemic status on glycated hemoglobin A1c (Hb1Ac) and homeostatic model assessment (HOMA) indices. The purpose of this study is to investigate how an eight-week high volume of moderate-intensity endurance plus resistance exercise combined with a medium carbohydrate, low fat, calorie-restricted, carbohydrate counting diet (MCCR) affects Hb1Ac and HOMA indices in individuals with prediabetes.MethodsTwenty-two young obese people (BMI ≥ 28 kg/m2) were divided into two groups: prediabetes intervention group (INT, n = 10) and normoglycemic control group (CON, n = 12). All participants received the MCCR dietary intervention and a high volume of moderate-intensity endurance plus resistance training, 6 days/week, 5 times/day, and 50 min/time, for 8 weeks. Body composition and circumference, serum lipids, fasting blood glucose (FPG), 2-h post-glucose (2 h-PG), fasting insulin (FINS), Hb1Ac, the insulin resistance (HOMA-IR), insulin sensitivity (HOMA-IS) and β-cell function (HOMA-β) indices were assessed.ResultsAfter exercise and dietary intervention, Hb1Ac, 2 h-PG, and FINS levels were significantly reduced in both the INT and CON groups (p < 0.05 or p < 0.01). HOMA-IR, HOMA-IS, and body fat percent were significantly improved in the INT group (p < 0.05), but HOMA-β was not observed. Additionally, Hb1Ac levels were significantly normalized in the prediabetic individuals, with a reversion rate of 71.43%, while there was no difference in FPG.ConclusionThe MCCR diet combined with an eight-week high volume of moderate-intensity endurance and resistance training is effective in reversing Hb1Ac and improving insulin sensitivity in young, obese adults with prediabetes.

Influence of altered serum and muscle concentrations of BDNF on electrophysiological properties of spinal motoneurons in wild-type and BDNF-knockout rats

The purpose of this study was to determine whether altered serum and/or muscle concentrations of brain-derived neurotrophic factor (BDNF) can modify the electrophysiological properties of spinal motoneurons (MNs). This study was conducted in wild-type and Bdnf heterozygous knockout rats (HET, SD-BDNF). Rats were divided into four groups: control, knockout, control trained, and knockout trained. The latter two groups underwent moderate-intensity endurance training to increase BDNF levels in serum and/or hindlimb muscles. BDNF and other neurotrophic factors (NFs), including glial cell-derived neurotrophic factor (GDNF), neurotrophin-3 (NT-3), nerve growth factor (NGF), and neurotrophin-4 (NT-4) were assessed in serum and three hindlimb muscles: the tibialis anterior (TA), medial gastrocnemius (MG), and soleus (Sol). The concentrations of tropomyosin kinase receptor B (Trk-B), interleukin-15 (IL-15), and myoglobin (MYO/MB) were also evaluated in these muscles. The electrophysiological properties of lumbar MNs were studied in vivo using whole-cell current-clamp recordings. Bdnf knockout rats had reduced levels of all studied NFs in serum but not in hindlimb muscles. Interestingly, decreased serum NF levels did not influence the electrophysiological properties of spinal MNs. Additionally, endurance training did not change the serum concentrations of any of the NFs tested but significantly increased BDNF and GDNF levels in the TA and MG muscles in both trained groups. Furthermore, the excitability of fast MNs was reduced in both groups of trained rats. Thus, changes in muscle (but not serum) concentrations of BDNF and GDNF may be critical factors that modify the excitability of spinal MNs after intense physical activity.

Long-Term Resistance–Endurance Combined Training Reduces Pro-Inflammatory Cytokines in Young Adult Females with Obesity

A sedentary lifestyle and an unhealthy diet increase the risk of obesity. People with obesity experience adipocyte hypertrophy and hyperplasia, which increases the production of proinflammatory cytokines, thereby increasing the risk of morbidity and mortality. Lifestyle modification using non-pharmacological approaches such as physical exercise prevents increased morbidity through its anti-inflammatory effects. The purpose of this study was to examine the effects of different types of exercise on decreased proinflammatory cytokines in young adult females with obesity. A total of 36 female students from Malang City aged 21.86 ± 1.39 years with body mass index (BMI) of 30.93 ± 3.51 kg/m2 were recruited and followed three different types of exercise interventions: moderate-intensity endurance training (MIET), moderate-intensity resistance training (MIRT), and moderate-intensity combined training (MICT). The exercise was performed at a frequency of 3x/week for 4 weeks. Statistical analysis was performed using the Statistical Package for Social Science (SPSS) version 21.0, using the paired sample t-test. The results revealed that serum IL-6 and TNF-α levels were significantly decreased between pre-training and post-training in the three types of exercise (MIET, MIRT, and MICT) (p ≤ 0.001). The percentage change in IL-6 levels from pre-training in CTRL was (0.76 ± 13.58%), in MIET was (-82.79 ± 8.73%), in MIRT was (-58.30 ± 18.05%), in MICT was (-96.91 ± 2.39%), and (p ≤ 0.001). There was a percentage change in TNF-α levels from pre-training in CTRL (6.46 ± 12.13%), MIET (-53.11 ± 20.02%), MIRT (-42.59 ± 21.64%), and MICT (-73.41 ± 14.50%), and (p ≤ 0.001). All three types of exercise consistently reduced proinflammatory cytokines such as serum levels of IL-6 and TNF-α.

Comparison of an exergame and a moderate-intensity endurance training intervention on physiological parameters

Background&#x0D; Exergames are interactive video games that stimulate an active, whole-body gaming experience (Best, 2013). By combining electronic entertainment with physical exercise, exergames offer novel opportunities to expand physical activity in different age groups and settings. Even though studies have found a significant increase in energy expenditure when playing exergames compared to normal video games, most games only induce low to moderate-intensity activity which is too low to result in relevant physical adjustments (Biddiss &amp; Irwin, 2010). This study assessed the effects of an 8-week exergame-training (EXT) in an innovative exergame called the ExerCube and compared it with a typical moderate-intensity endurance training (ET) intervention.&#x0D; Methods&#x0D; In total, 19 individuals (10 female; age 26.9 ±8.7 years; body mass index (BMI) 23.6 ±3.1 kg/m2) participated and were block randomized into an EXT group (n = 9) and an ET group (n = 10). Throughout the 8-week intervention period, the EXT group attended 20-30-minutes of EXT three times a week while the ET group completed 15-45-minutes of ET (jogging/cycling at 65-75% of maximal heart rate) three times a week. Before and after the intervention BMI, systolic and diastolic blood pressure, and VO2max (spiroergometry; start: 50 or 75 W; increment: 25 W/min) were assessed and compared (paired-samples t-test, ANOVA).&#x0D; Results&#x0D; Significant time × group interaction effects were found for VO2max (F(1,17) = 11.345; p = .004, ηp2 = .400). The EXT group revealed significant within-group effects in VO2max from pre (43.2 ±10.6 ml/kg*min) to post (46.9 ±10.9 ml/kg*min; p = .004, d = 1.308) while the ET group revealed no significant changes (pre: 39.4 ±5.4 ml/kg*min; post: 39.7 ±4.9 ml/kg*min; p = .466, d = .241). No significant time × group interaction effects were detected in systolic blood pressure (F(1,17) = .050; p = .825, ηp2 = .003) or diastolic blood pressure (F(1,17) = .005; p = .943, ηp2 = .000). However, there was a significant decrease in the peripheral systolic blood pressure from pre (122 ±10 mmHg) to post (117 ±12 mmHg; p = .034, d = .792) in the ET group but not in the EXT group (pre: 118 ±8; post: 114 ±7; p = .156, d = .523). Concerning BMI, no significant interaction effects (F(1,17) = 2.818; p = .111, ηp2 = .142) were detected.&#x0D; Conclusions&#x0D; The EXT seems to be more effective as conventional ET exercise approach to improve endurance performance. This is promising as exergame may develop intrinsic motivation/enjoyment for physical activity. Further studies confirming these findings and extending to psychological variables are needed.&#x0D; References&#x0D; Best, J. R. (2013). Exergaming in youth: Effects on physical and cognitive health. Zeitschrift für Psychologie, 221(2), 72-78. https://doi.org/10.1027/2151-2604/a000137&#x0D; Biddiss, E., &amp; Irwin, J. (2010). Active video games to promote physical activity in children and youth: A systematic review. Archives of Pediatrics &amp; Adolescent Medicine, 164(7), 664-672. https://doi.org/10.1001/archpediatrics.2010.104

Endurance Training with Moderate and Intense Intensity Along with the Consumption of Safflower Extract Improved the Oxidative Damage of Testicular Tissue Caused by Dexamethasone in Adult Male Rats

Background: Chronic use of dexamethasone, especially in athletes, is associated with reproductive disorders. Objectives: This study aimed to investigate the effect of high and moderate intensity endurance training along with the consumption of safflower extract on testicular oxidative damage and testosterone level in male rats treated with dexamethasone. Methods: A total of 45 male Wistar rats were divided into the controlled and treated groups. Dexamethasone was subcutaneously injected for five days at a dose of 8 mg/kg. High and moderate intensity exercises were performed for ten weeks (five days a week) at speeds of 28 and 34 m/min, respectively, on the treadmill. The safflower seed extract (500 mg/kg of the body weight) was administered daily during the training period by gavage. At the end of the given period, testicular tissue sampling was performed to measure malondialdehyde, total antioxidant capacity, Nrf2, and testosterone levels. Results: The results showed that high and moderate intensity exercises along with the consumption of safflower seed extract (P = 0.000) as well as high intensity exercise alone (P = 0.04) caused a significant increase in Nrf2 expression in animals receiving dexamethasone compared to the dexamethasone group. No significant change was observed in the testosterone level of the dexamethasone group compared to that of the control group. However, a significant change was detected in its level in some groups treated with moderate exercise, intense exercise, and safflower extract. Conclusions: It was concluded that moderate and high intensity endurance training, along with the consumption of safflower extract, had the potential to prevent infertility caused by dexamethasone.

Skeletal Muscle Satellite Cells in Sickle Cell Disease Patients and Their Responses to a Moderate-intensity Endurance Exercise Training Program.

We previously demonstrated that 8 weeks of moderate-intensity endurance training is safe and improves muscle function and characteristics of sickle cell disease (SCD) patients. Here, we investigated skeletal muscle satellite cells (SCs) in SCD patients and their responses to a training program. Fifteen patients followed the training program while 18 control patients maintained a normal lifestyle. Biopsies of the vastus lateralis muscle were performed before and after training. After training, the cross-sectional area and myonuclear content in type I fibers were slightly increased in the training patients compared to non-training patients. The SC pool was unchanged in type I fibers while it was slightly decreased in type II fibers in the training patients compared to non-training patients. No necrotic fibers were detected in patients before or after training. Therefore, the slight myonuclear accretion in type I fibers in trained SCD patients may highlight the contribution of SCs to training-induced slight type I fiber hypertrophy without expansion of the SC pool. The low training intensity and the short duration of training sessions could explain the low SC response to the training program. However, the lack of necrotic fibers suggests that the training program seemed to be safe for patients' muscle tissue.

Impact of continuous vs. interval training on oxygen extraction and cardiac function during exercise in type 2 diabetes mellitus

Exercise training improves exercise capacity in type 2 diabetes mellitus (T2DM). It remains to be elucidated whether such improvements result from cardiac or peripheral muscular adaptations, and whether these are intensity dependent. 27 patients with T2DM [without known cardiovascular disease (CVD)] were randomized to high-intensity interval training (HIIT, n = 15) or moderate-intensity endurance training (MIT, n = 12) for 24weeks (3sessions/week). Exercise echocardiography was applied to investigate cardiac output (CO) and oxygen (O2) extraction during exercise, while exercise capacity [([Formula: see text] (mL/kg/min)] was examined via cardiopulmonary exercise testing at baseline and after 12 and 24weeks of exercise training, respectively. Changes in glycaemic control (HbA1c and glucose tolerance), lipid profile and body composition were also evaluated. 19 patients completed 24weeks of HIIT (n = 10, 66 ± 11years) or MIT (n = 9, 61 ± 5years). HIIT and MIT similarly improved glucose tolerance (pTime = 0.001, pInteraction > 0.05), [Formula: see text] (mL/kg/min) (pTime = 0.001, pInteraction > 0.05), and exercise performance (Wpeak) (pTime < 0.001, pInteraction > 0.05). O2 extraction increased to a greater extent after 24weeks of MIT (56.5%, p1 = 0.009, pTime = 0.001, pInteraction = 0.007). CO and left ventricular longitudinal strain (LS) during exercise remained unchanged (pTime > 0.05). A reduction in HbA1c was correlated with absolute changes in LS after 12weeks of MIT (r = - 0.792, p = 0.019, LS at rest) or HIIT (r = - 0.782, p = 0.038, LS at peak exercise). In patients with well-controlled T2DM, MIT and HIIT improved exercise capacity, mainly resulting from increments in O2 extraction capacity, rather than changes in cardiac output. In particular, MIT seemed highly effective to generate these peripheral adaptations. NCT03299790, initially released 09/12/2017.

Moderate Intensity Exercise Training Improves Skeletal Muscle Performance in Symptomatic and Asymptomatic Statin Users

BackgroundThe combination of statin therapy and physical activity reduces cardiovascular disease risk in patients with hyperlipidemia more than either treatment alone. However, mitochondrial dysfunction associated with statin treatment could attenuate training adaptations. ObjectivesThis study determined whether moderate intensity exercise training improved muscle and exercise performance, muscle mitochondrial function, and fiber capillarization in symptomatic and asymptomatic statin users. MethodsSymptomatic (n = 16; age 64 ± 4 years) and asymptomatic statin users (n = 16; age 64 ± 4 years) and nonstatin using control subjects (n = 20; age 63 ± 5 years) completed a 12-week endurance and resistance exercise training program. Maximal exercise performance (peak oxygen consumption), muscle performance and muscle symptoms were determined before and after training. Muscle biopsies were collected to assess citrate synthase activity, adenosine triphosphate (ATP) production capacity, muscle fiber type distribution, fiber size, and capillarization. ResultsType I muscle fibers were less prevalent in symptomatic statin users than control subjects at baseline (P = 0.06). Exercise training improved muscle strength (P < 0.001), resistance to fatigue (P = 0.01), and muscle fiber capillarization (P < 0.01), with no differences between groups. Exercise training improved citrate synthase activity in the total group (P < 0.01), with asymptomatic statin users showing less improvement than control subjects (P = 0.02). Peak oxygen consumption, ATP production capacity, fiber size, and muscle symptoms remained unchanged in all groups following training. Quality-of-life scores improved only in symptomatic statin users following exercise training (P < 0.01). ConclusionsA moderate intensity endurance and resistance exercise training program improves muscle performance, capillarization, and mitochondrial content in both asymptomatic and symptomatic statin users without exacerbating muscle complaints. Exercise training may even increase quality of life in symptomatic statin users. (The Effects of Cholesterol-Lowering Medication on Exercise Performance [STATEX]; NL5972/NTR6346)

Modulation of Fibroblast Growth Factor-21 and βklotho Proteins Expression in Type 2 Diabetic Women with Non-alcoholic Fatty Liver Disease Following Endurance and Strength Training

Background: There is limited research on the effects of physical activity with moderate intensity on βklotho (BKL) and fibroblast growth factor-21 (FGF-21) proteins expression in diabetic patients with non-alcoholic fatty liver disease (NAFLD). Objectives: This study was aimed to determine the effects of eight weeks of endurance and resistance training on BKL and FGF-21 proteins expression in diabetic women with NAFLD. Methods: Forty-five diabetic women (age: 51 ± 8 years, height: 158 ± 2 cm, weight: 75 ± 8 kg) with NAFLD participated. The subjects were randomly divided into three groups, including control (n = 15), endurance training (n = 15), and resistance training (n = 15). The enzyme-linked immunosorbent assay (ELISA) was used to measure BKL and FGF-21 proteins. Two-way ANOVA with repeated measures was applied to determine differences at a significant level of P &lt; 0.05. Results: Eight weeks of endurance and resistance training reduced AST, ALT, and FGF-21 (25, 26, 19% and 13, 16, 13%, respectively) and increased BKL (16% and 18, respectively). However, in the variables of HDL, insulin, AST, ALT, FGF-21, and BKL, a significant difference was observed in the control group (P &lt; 0.05). Also, there was a significant difference between the control and training groups in BKL and FGF21 proteins expression (P &lt; 0.05), but no significant difference was observed between the two training groups (P &gt; 0.05). Conclusions: The results suggest that both moderate-intensity endurance and resistance training can modulate the destructive effects of type 2 diabetes and NAFLD on BKL and FGF-21 proteins expression, and there is no difference between the two training methods.

Research Progress of Mitochondrial Mechanism in NLRP3 Inflammasome Activation and Exercise Regulation of NLRP3 Inflammasome.

NLRP3 is an important pattern recognition receptor in the innate immune system, and its activation induces a large number of pro-inflammatory cytokines, IL-1β and IL-18 which are involved in the development of various diseases. In recent years, it has been suggested that mitochondria are the platform for NLRP3 inflammasome activation. Additionally, exercise is considered as an important intervention strategy to mediate the innate immune responses. Generally, chronic moderate-intensity endurance training, resistance training and high-intensity interval training inhibit NLRP3 inflammasome activation in response to various pathological factors. In contrast, acute exercise activates NLRP3 inflammasome. However, the mechanisms by which exercise regulates NLRP3 inflammasome activation are largely unclear. Therefore, the mechanism of NLRP3 inflammasome activation is discussed mainly from the perspective of mitochondria in this review. Moreover, the effect and potential mechanism of exercise on NLRP3 inflammasome are explored, hoping to provide new target for relevant research.

Effect Of High-intensity Vs. Moderate-intensity Endurance Training On Critical Power In Untrained, Young Adults

Effect Of High-intensity Vs. Moderate-intensity Endurance Training On Critical Power In Untrained, Young Adults

PGC-1α regulates myonuclear accretion after moderate endurance training.

The transcriptional demands of skeletal muscle fibres are high and require hundreds of nuclei (myonuclei) to produce specialised contractile machinery and multiple mitochondria along their length. Each myonucleus spatially regulates gene expression in a finite volume of cytoplasm, termed the myonuclear domain (MND), which positively correlates with fibre cross-sectional area (CSA). Endurance training triggers adaptive responses in skeletal muscle, including myonuclear accretion, decreased MND sizesand increased expression of the transcription co-activator peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α). Previous work has shown that overexpression of PGC-1α in skeletal muscle regulates mitochondrial biogenesis, myonuclear accretion and MND volume. However, whether PGC-1α is critical for these processes in adaptation to endurance training remained unclear. To test this, we evaluated myonuclear distribution and organisation in endurance-trained wild-type mice and mice lacking PGC-1α in skeletal muscle (PGC-1α mKO). Here, we show a differential myonuclear accretion response to endurance training that is governed by PGC-1α and is dependent on muscle fibre size. The positive relationship of MND size and muscle fibre CSA trended towards a stronger correlation in PGC-1a mKO versus control after endurance training, suggesting that myonuclear accretion was slightly affected with increasing fibre CSA in PGC-1α mKO. However, in larger fibres, the relationship between MND and CSA was significantly altered in trained versus sedentary PGC-1α mKO, suggesting that PGC-1α is critical for myonuclear accretion in these fibres. Accordingly, there was a negative correlation between the nuclear number and CSA, suggesting that in larger fibres myonuclear numbers fail to scale with CSA. Our findings suggest that PGC-1α is an important contributor to myonuclear accretion following moderate-intensity endurance training. This may contribute to the adaptive response to endurance training by enabling a sufficient rate of transcription of genes required for mitochondrial biogenesis.

The Effect of Six Weeks of Moderate-Intensity Endurance Training on Serum Levels of Klotho and Expression of the Fibroblast-23 Growth Factor Gene (FGF23) in the Hearts of Diabetic Rats: An Experimental Study

The Effect of Six Weeks of Moderate-Intensity Endurance Training on Serum Levels of Klotho and Expression of the Fibroblast-23 Growth Factor Gene (FGF23) in the Hearts of Diabetic Rats: An Experimental Study

Effects of Six-Week Endurance Training in Liver Enzymes, Lipid Profile, Interleukin-6 and High-Sensitivity C-Reactive Protein (hs-CRP) Responses in People with a Family History of Coronary Artery Disease

Background: Research on the effects of moderate-intensity endurance training on inflammatory indices and cardiovascular risk factors is limited. Objectives: The aim of this study was to determine the effects of six weeks of endurance training on liver enzymes, body composition, lipid profile, interleukin-6, and C-reactive protein responses in people with a family history of coronary artery disease (CAD). Methods: Thirty men (30 - 45years) participated in this study who were divided into two groups, including the control group (n = 10) and training group (n = 20) systematically. The training group program consisted of running on a treadmill for 30 minutes with an intensity of 55 to 60% of maximum heart rate. These training programs were continued three times a week for six weeks. The control group did not perform any exercise program. ELISA technique was used to measure lipid profile, liver enzymes, interlukin-6 (IL-6), and high-sensitivity C-reactive protein (hs-CRP), respectively. Results: The results showed that the variables of weight, liver enzymes, lipid profile, IL-6, and hs-CRP were significantly different from the pre-test (P &lt; 0.05). Also, in between-group comparison, the results showed that except for the variables of fat percentage and BMI in the other variables of the study, a significant difference was observed (P &lt; 0.05). Conclusions: The results of this study suggest that moderate-intensity endurance training could be used as a useful way to reduce weight, levels of inflammatory markers, liver enzymes and cardiovascular risk factors in people with a family history of CAD.