Moderate Congruence Research Articles

The congruence and interrelationships of family resilience in couples coping with colorectal cancer: A cross-sectional study from a dyadic perspective.

To assess the congruence and interdependent impacts of family resilience in colorectal cancer couples using the Walsh Family Resilience Model, focusing on coping domains of the shared beliefs system, organizational patterns, and communication/problem-solving. 220 colorectal cancer couples were recruited from two hospitals in China, assessing family resilience, hope, family sense of coherence, spiritual well-being, social support, and couple communication quality. Congruence was assessed using intraclass correlation coefficients and paired t-tests. Pearson correlations, multiple stepwise linear regression, and structural equation modeling examined the effects of coping domains on family resilience. Actor-Partner Independence Model was used to further explore actor and partner effects of these coping domains on family resilience in colorectal cancer couples. Moderate congruence in family resilience was observed, with spousal caregivers reporting higher family resilience. Key coping domains, including shared beliefs system (hope, family sense of coherence, spiritual well-being), organizational patterns (social support), and mutual communication/problem-solving, were significant predictors of family resilience. Importantly, both patients' and spousal caregivers' coping domains exhibited notable actor and partner effects on family resilience of their own family resilience and partners. This study highlights the interdependent influences on family resilience in colorectal cancer couples, supporting family-centered interventions to strengthen positive coping mechanisms.

Generalist species exhibit more genetic structure in comparison to a habitat specialist: Evidence from a phylogeographic study of two freshwater crabs (Decapoda: Potamonautidae: Potamonautes), implications for habitat conservation.

During the present study, the phylogeography of the wetland specialist, freshwater crab, Potamonautes flavusjo, was investigated and compared to that of the ubiquitous, generalist, P. sidneyi, using DNA sequence data from the mitochondrial cytochrome oxidase subunit one (COI). We inferred the evolutionary history of each species and compared their population-level genetic structure by constructing haplotype networks and using an analysis of molecular variation. Additionally, we explored the evolutionary relationship between southern African lentic and lotic freshwater crab species by examining the usefulness of carapace attributes in relation to genetic indices and a species' assumed dispersal capacity. In the lentic species, P. flavusjo, a single interconnected haplocluster characterized by shared haplotypes was observed, suggesting marked maternal dispersal, a result corroborated by the low F ST values. In contrast, for the lotic species, P. sidneyi two distinct haploclusters and marked genetic differentiation was observed indicating the absence of maternal dispersal, a result corroborated by the high F ST values. The phylogenetic relationship in P. sidneyi was further investigated using a maximum likelihood and Bayesian inference analyses with the addition of sequence data from the mitochondrial 16S rRNA locus to estimate divergence times. Three species delimitation methods (ASAP, PTP, and bGMYC) were used to explore the presence of distinct lineages in P. sidneyi. The phylogenetic results indicated that within P. sidneyi two clades were present, while divergence time estimates suggest cladogenesis during the middle to late Pleistocene. The species delimitation methods used showed moderate congruence, however all oversplit the number of putative lineages. Our results indicated that P. sidneyi is a species complex comprised of two cryptic lineages, occurring in sympatry contemporarily possibly alluding to secondary contact. Carapace height was found to have no discernible influence on the genetic indices and presumed dispersal capabilities of mainland southern African freshwater crab species. The importance of our results are discussed in terms of conservation of freshwater habitats.

Development and evaluation of a clinical guideline for a paediatric telemedicine service in a low-resource setting

ObjectiveTo develop and evaluate a guideline for a paediatric telemedicine and medication delivery service (TMDS).MethodsA clinical guideline for paediatric telemedicine was derived from the World Health (WHO) Organization Integrated Management...

Intergenic region polymorphism analysis: a novel genotyping method for Klebsiella pneumoniae.

The ability to distinguish between Klebsiella pneumoniae strains is critical for outbreak investigations. A new typing method, intergenic region polymorphism analysis (IRPA), was developed, validated, and the discriminatory power was determined by comparison with multiple-locus variable-number tandem repeat analysis (MLVA) in this study. This method is based on the idea that every IRPA locus (polymorphic fragment of intergenic regions present in one strain but not in other strains or different fragment sizes in other strains) could divide strains into different genotypes. A 9-loci IRPA scheme was designed to type 64K. pneumoniae isolates. Five IRPA loci were identified that conferred the same level of discrimination as the 9-loci initially examined. Among these K. pneumoniae isolates, 7.81% (5/64), 6.25% (4/64), 4.96% (3/64), 9.38% (6/64), and 1.56% (1/64) were capsular serotypes K1, K2, K5, K20, and K54, respectively. The discriminatory power of the IRPA method was better than that of MLVA expressed in Simpson's index of diversity (SI) at 0.997 and 0.988, respectively. The congruent analysis of the IRPA method and MLVA showed moderate congruence between the two methods (AR=0.378). The AW indicated that if IRPA data are availabl, one can accurately predict the MLVA cluster. The IRPA method was found to have higher discriminatory power than MLVA and allowed for simpler band profile interpretation. The IRPA method is a rapid, simple, and high-resolution technique for molecular typing of K. pneumoniae.

Subtyping of Campylobacter coli isolated from raw poultry meat in retail markets using amplified intergenic locus polymorphism - A novel rapid subtyping method

In order to provide more phylogenetic information of Campylobacter coli in large-scale epidemiological investigation, this work was undertaken to develop a novel genotyping method based on amplified intergenic locus polymorphism (AILP), by using pulsed-field gel electrophoresis (PFGE; using SmaI enzymes) as control. Eleven pairs of primers were selected to type C. coli strains for this purpose. A total of 68 C. coli isolates recovered from 51 retail raw chicken and 37 retail raw duck were subtyped. The Simpson's index of diversity (SID) of AILP and PFGE, as well as the adjusted Rand index (AR) and the adjusted Wallace coefficient (AW) between AILP and PFGE, were calculated. The new AILP method differentiated 68 C. coli isolates into 55 different subtypes (SID = 0.993), compared with 46 different profiles obtained from PFGE (SID = 0.980). The SID value of the AILP method was improved with the increasing number of primers, and a combination of 7 loci was selected as the optimal combination. The congruent analysis of the AILP method and PFGE showed moderate congruence between the two methods (AR = 0.462). The AW indicated that if AILP data is the available one can confidently predict the PFGE cluster. The results of this study showed that the AILP method had higher discrimination than PFGE and also allowed for significant reduction in time and cost.

Behaviour-based pain scales: Validity and interrater reliability of BPS-NI and PAINAD-G on general wards.

Measuring pain intensity in patients unable to communicate is a challenge on general wards. Observation-based pain scoring tools have been used for patients with dementia or in critically ill intensive care unit (ICU) patients. However, there is no established or validated assessment tool for non-ICU patients without dementia related cognitive deficiencies who cannot communicate. The "Behavioural Pain Scale Non-Intubated" (BPS-NI) and the "Pain Assessment In Advanced Dementia-German" (PAINAD-G) are potential tools to fill this gap. This study was performed with verbal non-ICU patients on general wards at Charité Berlin. Two assessors independently rated pain intensity using the BPS-NI and the PAINAD-G along with patients' self-ratings on the Numeric Rating Scale (NRS). The interrater-reliability of BPS-NI and PAINAD-G was calculated and ROC-analyses were performed to identify cut-off values for medium and intense pain for each score. Effectiveness was calculated using percentage agreement. In total, 126 patients were included into analysis. The BPS-NI showed substantial congruence in interrater-reliability (Cohens-Kappa 0.71), whereas the PAINAD-G showed moderate congruence (Kappa 0.48). Based on ROC-analyses, for medium pain levels a cut-off 4 (BPS-NI) or 2 (PAINAD-G) and for severe pain levels cut-off 5 (BPS-NI) and 3 (PAINAD-G) would result in good accordance with self-reported NRS for pain. The BPS-NI shows a good validity in measuring pain intensity in patients on general wards and may possibly be used for patients unable to communicate. Using defined cut-off values for BPS-NI and PAINAD-G, clinically relevant pain intensities in patients can reliably be detected. Measuring the pain intensity in patients unable to communicate is a common challenge on general wards. This study assessed reliability and applicability BPS-NI and the PAINAD-G in a general ward setting. Furthermore, it provides cut-off values in order to estimate pain intensity and support analgesic response.

Identifying Potential Attrition during the Residency Applicant Screening Process Using a Situational Judgment Test

As USMLE Step 1 moves to pass/fail, residency programs are seeking alternate interview selection processes. Attrition in general surgery is reported as high as 26%. Thus, it is important to ensure that programs are selecting and matching applicants with shared values. Situational judgment tests (SJTs) measure educational and cultural values by posing ambiguous situations and individuals rate the effectiveness of possible reactions. SJTs have previously been shown to identify job applicants with shared values while promoting diversity. Scoring categories are high, moderate, or low values congruence. We sought to explore predictive validity of the SJT relative to program attrition. Residents who matched into our program between 2018 and 2021 completed the SJT. We tracked attrition. UT Health San Antonio, Texas PARTICIPANTS: Fifty-six categorical general surgery residents RESULTS: Per SJT ratings, the numbers of residents who had high, moderate, and low values congruence were 27, 16, and 13, respectively. Attrition numbers for residents who scored high and moderate congruence were similar, indicating that these ratings were indistinguishable. As such, we combined those 2 categories to create a 2 × 2 matrix and used signal detection theory as a framework for analysis. Overall attrition was 16.1% (9/56). Of the 43 residents who scored high or moderate congruence, 90.7% remained in the program. There was a 9.3% chance of attrition for these residents. Of the 13 residents who scored low congruence, 38.5% attrited. While scoring as low congruence on the SJT does not definitively indicate attrition, it does indicate that attrition is 4.14 times more likely for these residents (chi-square, p = 0.0121). One of the most important aspects of residency applicant selection and interviewing is mitigating risk by identifying applicants who carry a high risk of attrition. The SJT significantly identifies at-risk applicants.

Host phylogeny, diet, and habitat differentiate the gut microbiomes of Darwin\u2019s finches\xa0on Santa Cruz Island

Darwin’s finches are an iconic example of an adaptive radiation with well-characterized evolutionary history, dietary preferences, and biogeography, offering an unparalleled opportunity to disentangle effects of evolutionary history on host microbiome from other factors like diet and habitat. Here, we characterize the gut microbiome in Darwin’s finches, comparing nine species that occupy diverse ecological niches on Santa Cruz island. The finch phylogeny showed moderate congruence with the microbiome, which was comprised mostly of the bacterial phyla Firmicutes, Actinobacteria, and Proteobacteria. Diet, as measured with stable isotope values and foraging observations, also correlated with microbiome differentiation. Additionally, each gut microbial community could easily be classified by the habitat of origin independent of host species. Altogether, these findings are consistent with a model of microbiome assembly in which environmental filtering via diet and habitat are primary determinants of the bacterial taxa present with lesser influence from the evolutionary history between finch species.

Does self-acceptance captured by life narratives and self-report predict mental health? A longitudinal multi-method approach

We aimed to investigate the validity of different self-acceptance measures to predict mental health. Self-acceptance and negative life events, assessed via self-report and rated from life narratives (N = 149), served as predictors of mental health at baseline (T1) and four years later (T2). Path models showed distinguishable, complementary effects of self-reports and other-ratings. A moderate congruence of self- and other-ratings of self-acceptance was observed. Exploratory analyses revealed an association of self-acceptance with emotional words in life narratives. Controlling for negative life events, a positive link and a moderating effect of self-acceptance on mental health at T1, but no prediction of mental health at T2, were found. The study connects research on personality and narratives from a methodological and health psychological perspective.

Divergence among maize genotypes with different kernel types according to SSR marker analysis

Panels of diverse materials have proven to be very useful in evaluation of the organization of genetic diversity available for breeding at phenotypic and genotypic levels. In this study, a panel consisting of several groups of maize inbreds was tested using microstelite markers. The aim of this study was to test genetic diversity and define population structure of twenty-four genotypes differing in kernel type (dent, flint, popcorn, sweet maize) and kernel color (white, yellow, orange) with 21 SSRs. Genetic diversity parameters such as number of alleles, gene diversity, observed heterozygosity, PIC (Polymorphism Information Content) and number of unique alleles were determined. In addition, genetic distances according to Rogers distance were calculated. The values for all parameters were high, reflecting high genetic divergence of analyzed germplasm. Distance matrix based on UPGMA cluster analysis showed moderate congruence to available pedigree/origin or kernel type/color data. Genetic structure of tested genotypes was defined using Bayesian model-based clustering, without a considerable difference in comparison to cluster data analysis. The determined diversity parameters along with the results concerning genetic structure analysis provided a valuable information for improved selection efficiency.

Water availability drives habitat quality for the butterfly Plebejus argus in a Mediterranean sand dune landscape

For terrestrial species, habitat is frequently defined as physical patches of a certain land cover type in a matrix of non-habitat. However, the appropriate definition of habitat quality requires more detailed information on the resources and conditions that are necessary for the maintenance of the species (‘resource-based habitat approach’). In arthropods, their life cycle comprises several clearly differentiated stages that may depend on different resources and conditions for successful development; in turn, males and females may have different habitat requirements due to their different behaviours. Here we examine the habitat quality for males, females and eggs of the butterfly Plebejus argus in a continuous sand dune landscape in southern Spain. For both sexes, abundance was related to host plant density (negatively) and distance to heathland vegetation (a surrogate for higher water availability; negatively for males and hump-shaped for females); and, for males only, positively with flower presence. Egg abundance was positively related to mutualistic ant frequency and flower presence, and negatively to distance to heathland. Hence, female and male adults and eggs of P. argus partly differed in their dependence on resources and conditions, although distance to wetter areas of heathland was the common variable for all models. This suggests that soil humidity is the key variable for P. argus habitat quality, probably through both direct effects on individuals and indirect effects on interacting species (mutualistic ants and nectar sources). As a result, we found a high spatial congruence in habitat quality between males and females, and a moderate congruence between adults and eggs.

Evaluation of a Modified Italian European Prospective Investigation into Cancer and Nutrition Food Frequency Questionnaire for Individuals with Celiac Disease

BackgroundTo date, it is unclear whether individuals with celiac disease following a gluten-free (GF) diet for several years have adequate intake of all recommended nutrients. Lack of a food frequency questionnaire (FFQ) for individuals with celiac disease could be partly responsible for this still-debated issue. ObjectiveThe aim of the study is to evaluate the performance of a modified European Prospective Investigation into Cancer and Nutrition (EPIC) FFQ in estimating nutrient and food intake in a celiac population. DesignIn a cross-sectional study, the dietary habits of individuals with celiac disease were reported using a modified Italian EPIC FFQ and were compared to a 7-day weighed food record as a reference method. Participants/settingA total of 200 individuals with histologically confirmed celiac disease were enrolled in the study between October 2012 and August 2014 at the Center for Prevention and Diagnosis of Celiac Disease (Milan, Italy). Main outcome measuresNutrient and food category intake were calculated by 7-day weighed food record using an Italian food database integrated with the nutrient composition of 60 GF foods and the modified EPIC FFQ, in which 24 foods were substituted with GF foods comparable for energy and carbohydrate content. Statistical analyses performedAn evaluation of the modified FFQ compared to 7-day weighed food record in assessing the reported intake of nutrient and food groups was conducted using Spearman’s correlation coefficients and weighted κ. ResultsOne hundred individuals completed the study. The Spearman's correlation coefficients of FFQ and 7-day weighed food record ranged from .13 to .73 for nutrients and from .23 to .75 for food groups. A moderate agreement, which was defined as a weighted κ value of .40 to .60, was obtained for 30% of the analyzed nutrients, and 40% of the nutrients showed values between .30 and .40. The weighted κ exceeded .40 for 60% of the 15 analyzed food groups. ConclusionsThe modified EPIC FFQ demonstrated moderate congruence with a weighed food record in ranking individuals by dietary intakes, particularly food groups.

Therapeutic bond judgments: Congruence and incongruence.

The present study had 2 aims: (a) to implement West and Kenny's (2011) Truth-and-Bias model to simultaneously assess the temporal congruence and directional discrepancy between clients' and therapists' ratings of the bond facet of the therapeutic alliance, as they cofluctuate from session to session; and (b) to examine whether symptom severity and a personality disorder (PD) diagnosis moderate congruence and/or discrepancy. Participants included 213 clients treated by 49 therapists. At pretreatment, clients were assessed for a PD diagnosis and completed symptom measures. Symptom severity was also assessed at the beginning of each session, using client self-reports. Both clients and therapists rated the therapeutic bond at the end of each session. Therapists and clients exhibited substantial temporal congruence in their session-by-session bond ratings, but therapists' ratings tended to be lower than their clients' across sessions. Additionally, therapeutic dyads whose session-by-session ratings were more congruent also tended to have a larger directional discrepancy (clients' ratings being higher). Pretreatment symptom severity and PD diagnosis did not moderate either temporal congruence or discrepancy at the dyad level; however, during sessions when clients were more symptomatic, therapist and client ratings were both farther apart and tracked each other less closely. Our findings are consistent with a "better safe than sorry" pattern, which suggests that therapists are motivated to take a vigilant approach that may lead both to underestimation and to attunement to fluctuations in the therapeutic bond.

Identification and characterisation of new Campylobacter group III phages of animal origin.

Campylobacter-specific bacteriophages (phages) are considered as an alternative intervention strategy to decrease the level of poultry contamination with Campylobacter, a leading cause of gastroenteritis worldwide. Eradication efficiency depends primarily on phage-host interaction mediated by phage tail-spike proteins and bacterial receptors. Here, this interaction was characterised using tail-spike gene sequence analysis, phage neutralisation by antiserum and host range analysis of newly isolated group III Campylobacter phages with 68 Campylobacter jejuni and Campylobacter coli strains. Three different groups of phages were obtained using antibody neutralisation assay, and they were further divided according to polymorphisms observed within tail fibre sequences and host range. Only moderate congruence was observed between these criteria with notable exception of two phages. The infection relied on capsule in all phages isolated, and flagella were found to influence phage propagation on agar plates, but not in broth. Their specificity was more C.jejuni oriented with tendency to lyse human isolates more efficiently. Additionally, natural resistance of C.jejuni to phages did not correlate with their antibiotic resistance patterns. These findings provide new insights into Campylobacter-phage interaction.

Inferring genome-wide recombination landscapes from advanced intercross lines: application to yeast crosses.

Accurate estimates of recombination rates are of great importance for understanding evolution. In an experimental genetic cross, recombination breaks apart and rejoins genetic material, such that the genomes of the resulting isolates are comprised of distinct blocks of differing parental origin. We here describe a method exploiting this fact to infer genome-wide recombination profiles from sequenced isolates from an advanced intercross line (AIL). We verified the accuracy of the method against simulated data. Next, we sequenced 192 isolates from a twelve-generation cross between West African and North American yeast Saccharomyces cerevisiae strains and inferred the underlying recombination landscape at a fine genomic resolution (mean segregating site distance 0.22 kb). Comparison was made with landscapes inferred for a similar cross between four yeast strains, and with a previous single-generation, intra-strain cross (Mancera et al., Nature 2008). Moderate congruence was identified between landscapes (correlation 0.58–0.77 at 5 kb resolution), albeit with variance between mean genome-wide recombination rates. The multiple generations of mating undergone in the AILs gave more precise inference of recombination rates than could be achieved from a single-generation cross, in particular in identifying recombination cold-spots. The recombination landscapes we describe have particular utility; both AILs are part of a resource to study complex yeast traits (see e.g. Parts et al., Genome Res 2011). Our results will enable future applications of this resource to take better account of local linkage structure heterogeneities. Our method has general applicability to other crossing experiments, including a variety of experimental designs.

Subtypes of Irritable Bowel Syndrome Based on Abdominal Pain/Discomfort Severity and Bowel Pattern

Irritable bowel syndrome (IBS) has traditionally been classified by stooling pattern (e.g., diarrhea-predominant). However, other patterns of symptoms have long been recognized, e.g., pain severity. Our objective was to examine the utility of subtyping women with IBS based on pain/discomfort severity as well as predominant bowel pattern. Women (n=166) with IBS completed interviews, questionnaires, and kept a diary for 28days. Rome II questionnaire items eliciting the past year recall of hard and loose stools, and frequency and severity of abdominal pain or discomfort were used to classify participants into six subtypes-three bowel pattern categories by two pain/discomfort severity categories. Concordance of these subtypes with corresponding diary items was examined. Analysis of variance (ANOVA) tested the relationship of bowel pattern and pain categories to measures of quality of life and symptoms. There is moderate congruence of the retrospective classification of bowel pattern and pain/discomfort severity subtypes with prospectively reported stool frequency and consistency and pain severity. Quality of life, impact of IBS on work and daily activities, and cognitive beliefs about IBS differed significantly based on abdominal pain/discomfort category but not on predominant bowel pattern. There is evidence of an interaction, with the effect of pain severity being strong in the IBS-diarrhea and IBS-mixed groups, but this was absent in the IBS-constipation group. Similar results hold for most diary symptoms, except for those directly related to bowel pattern. Overall, the distress of IBS is more strongly related to the severity of abdominal pain/discomfort than is the predominant stool pattern in patients with IBS. Categorizing IBS patients by abdominal pain/discomfort severity in conjunction with predominant bowel pattern may be useful to clinicians and researchers in developing more effective management.

Research considerations: nursing home quality perceptions.

1. One of our nation's most challenging health care problems is providing quality care for nursing home residents. Those of us who desire to evaluate and to improve the quality of care provided in a nursing home must attempt to define quality in specific terms, even though it is a product of many factors. 2. Ideally, there should be congruence between resident and staff perceptions of the importance and occurrence of indicators of quality in a nursing home setting. However, minimal to moderate congruence of perceptions was found in this study. 3. The fact that the majority of 30 residents in two nursing homes considered these 17 quality indicators to be important further validates them as markers of the quality of care in nursing homes. Use of a comprehensive quality assurance process that includes some or all of these indicators for evaluating and improving care in nursing homes could demonstrate a real commitment to quality that would go beyond only responding to the requirements of consumers and external regulators.

Dimensional analysis of the MMPI alexithymia scale

Recently, the alexithymia construct has emerged as a heuristically useful personality construct in the explanation of the pathogenesis of a variety of physical illnesses. Research that has evaluated the alexithymia construct, however, has been conducted with little attention to the psychometric properties of the various scales constructed to measure it. This study investigated the underlying dimensions and factor structure stability of the MMPI Alexithymia scale (MMPI-A). The MMPI-A was administered to 552 undergraduates, and the data then were split randomly into two samples. Factor analysis of both samples produced a three-factor solution with little theoretical relevancy to the alexithymia construct. Comparisons of the factor structures from the two samples revealed only moderate congruence, which indicates an unstable factor structure.

Amino acid sequence versus morphological data and the interordinal relationships of mammals.

To a large extent, the mutual affinities of the mammalian orders continue to puzzle systematists, even though comparative anatomy and amino acid sequencing offer a massive data base from which these relationships could potentially be adduced. In the present paper the consistency index--the number of character states less the number of characters in a data set, divided by the total number of changes in the character states on a cladogram--was used to examine the relative resolving powers of recently published morphological and molecular-sequence data. Consistency indices were calculated for previously published alpha crystallin A chain and myoglobin amino acid-sequence cladograms and for four original amino acid-sequence cladograms (alpha crystallin A, myoglobin, and alpha and beta hemoglobin); these were found to be comparable to the consistency indices of morphologically based cladograms. Qualitative comparisons between the morphologically based and molecularly based trees were also made; only moderate congruence between the two was observed. Moreover, there was a general lack of congruence between the cladograms specified by each of the four proteins. Amino acid-sequence and morphological data agreed on the placement of edentates as an early eutherian offshoot and on the grouping of hyracoids, proboscideans, and sirenians. Otherwise there was only limited congruence: morphology strongly supported the grouping of lagomorphs and rodents and the alliance of pholidotes and edentates, but sequence analyses did not. The placement of tubulidentates differed widely among proteins. Morphology indicated the close association of sirenians with proboscideans; proteins suggested a pairing of sirenians with hyracoids. Sequence data did not identify many (morphologically well-diagnosed) orders as monophyletic (e.g., Lagomorpha).(ABSTRACT TRUNCATED AT 250 WORDS)