Moderate Body Condition Score Research Articles

Nutritional Strategies to Promote Bovine Oocyte Quality for In Vitro Embryo Production: Do They Really Work?

The ability of bovine oocytes to reach the blastocyst stage (i.e., embryo with around 150 cells in cattle) in vitro can be affected by technical (e.g., culture medium used) and physiological factors in oocyte donors (e.g., age, breed). As such, the nutritional status of oocyte donors plays a significant role in the efficiency of in vitro embryo production (IVEP), and several nutritional strategies have been investigated in cattle subjected to ovum pick-up (OPU). However, there is no clear consensus on the reliability of nutritional schemes to improve IVEP in cattle. Available evidence suggests that a moderate body condition score (i.e., 3 in a 1-5 scale) in cattle is compatible with a metabolic microenvironment in ovarian follicles that will promote embryo formation in vitro. The usefulness of fatty acid and micronutrient supplementation to improve IVEP in cattle is debatable with the current information available. Overall, the supply of maintenance nutritional requirements according to developmental and productive stage seems to be enough to provide bovine oocyte donors with a good chance of producing embryos in vitro. Future nutrition research in cattle using OPU-IVEP models needs to consider animal well-being aspects (i.e., stress caused by handling and sampling), which could affect the results.

12 Metabolic gene expression between emaciated and moderately conditioned horses across various tissue types

Effective refeeding of emaciated horses requires strategic management, including knowledge of tissue specific contributions to metabolic processes. To determine the differences in metabolic activity among body condition score (BCS) groups, 8 horses donated to this study were evaluated for BCS (1–9 scale,1 = emaciated and 9 = obese); where horses with BCS lower than 3 were considered emaciated (n = 4), and those with a BCS between 4 and 6 were considered moderate (n = 4). All horses were humanely euthanized using sodium barbiturate overdose, and during necropsy, tissue samples across 4 types were collected: muscle (M: cardiac and skeletal), fat (F: mesenteric, neck crest, omental, and peritoneal), gastrointestinal tract (G: stomach, duodenum, jejunum, ileum, and cecum), and organs (O: liver, kidney, and spleen). Samples were placed in liquid nitrogen and stored at −80°C before isolating RNA for gene expression analysis. Relative gene expression (using 2^-DCt) of RPL32, GLUT4, CPT1, and PDK4 were measured across tissues and between F, G, O and M, using geometric mean of GAPDH and TUBA4A as reference genes. Class, tissue, and BCS effects on PCR data were analyzed using generalized linear mixed models with breed and individual animals as random block effects. Shapiro-Wilk and Levine's test were used to determinenormality, and ranked transformation applied where appropriate. Post hoc multiple comparisons were performed with Tukey's adjustment and significance was identified at P < 0.05 (SAS 9.4, Cary, NC). No differences were detected between BCS groups (P > 0.05). Tissue classification had a significant effect on each target gene expression, where CPT 1 had lower expression in O [0.170 (0.440), 2^-DCt median (interquartile range), P < 0.001] than F [0.100 (0.125)] and M [0.06 (0.150), P < 0.0001). No differences were detected for tissue classification for GLUT4, PDK4 or RPL32 (P > 0.05). Further, no individual tissue, BCS, or interactions were identified across all tissue type and gene pairs (P > 0.05). Despite known physiologic and physical differences in emaciated versus moderate BCS equids, gene expression was relatively unaffected in this study, which may be due to the tightly controlled nature of each gene to maintaining life. Knowledge of gene activity in the horse, and especially across tissue profiles and in the presence of sodium barbiturate is limited within the existing literature. Further investigation into tissue-specific differences may aid in understanding metabolic changes associated with BCS and should be evaluated in the presence and absence of euthanasia solution to fully elicit potential effects.

Effects of Nutritional Plane at Breeding on Feed Intake, Body Weight, Condition Score, Mass Indexes, and Chemical Composition, and Reproductive Performance of Hair Sheep.

This study was conducted to assess effects of the nutritional plane before and during breeding on feed intake, body weight (BW) change, reproductive performance, body condition score (BCS), body mass indexes (BMI), and body composition of three breeds of hair sheep. Twenty-five Dorper, 27 Katahdin, and 33 St. Croix ewes were allocated to groups and treatments based on initial BW and age. Supplementation treatments were soybean meal fed at 0.16% BW (LS) and a mixture of 25% soybean meal and 75% ground corn given at 0.8% BW (HS; dry matter basis) for 88 to 102 days including 17 days after breeding. Wheat straw intake (% BW) was greater (p < 0.01) for LS than for HS. Average daily gain and BCS change were similar among breeds, but both were greater for HS vs. LS. Birth rate, litter size, total litter birth weight, gestation length, and number of services were similar among breeds (p > 0.05), although individual lamb birth weight was lower for St. Croix than for Katahdin (p < 0.05). None of these reproductive variables were influenced by treatment or the breed × treatment interaction (p > 0.05). There were no breed differences in whole body concentrations of water, fat, protein, ash, or energy in week 0 or 4, but in 8 week concentrations of water, protein, and ash were greatest among breeds for St. Croix (p < 0.05) and levels of fat and energy were lower for St. Croix than for Dorper (p < 0.05). In week 8, total amounts and concentrations of fat and energy were greater for HS vs. LS and those of water, protein, and ash were less for HS (p < 0.05). There were interactions (p < 0.05) between treatment and period in blood concentrations of cholesterol and non-esterified fatty acids and between breed, treatment, and period in level of triglycerides and total antioxidant capacity. The concentration of cortisol was greater for HS vs. LS (p < 0.05). In conclusion, supplementation did not influence reproductive performance despite differences in BW and BCS change, which may be due to the initial moderate BCS and greater wheat straw intake for the LS treatment.

Prevalence and risk factors associated with equine strongylosis in Baybay City, Leyte, Philippines

Strongylosis is a pressing equine health problem. This study determined the prevalence and risk factors associated with strongylosis in horses in Baybay City, Leyte, based on its egg morphology. A total of 263 horse fecal samples were collected and examined. Nematode eggs were identified using Modified McMaster technique. Other relevant epidemiological data associated with strongyle infections were also collected using a structured questionnaire. Descriptive statistics and logistic regression analyses were carried out to determine the prevalence and risk factors associated with strongylosis in horses. Results showed an overall prevalence of 97.72% (257/263; 95% CI=95.10-99.16); of which, 92.78% (244/263; 95% CI=91.18-94.37) were cyathostomins and 67.30% (177/263; 95% CI=64.41-70.19) were Strongylus sp. Logistic regression analyses revealed a significant association between Strongylus sp. infection and moderate body condition score (p-value=0.0006), housing in a shed (p-value=0.0255), and tethering of the horse (p-value=0.0116). The high prevalence rate of equine strongyle infection in Baybay City, Leyte, could indicate the neglect and underestimation of the disease for a considerable period of time. Thus, active clinico-epidemiological investigations are warranted in order to design and establish cost-effective and sustainable control and preventive approaches.

Factors affecting placental size in beef cattle: Maternal and fetal influences

Our objectives were to determine the effects of dam body condition score (BCS), age of dam, and calf sex on placental size and the relationships between dam body weight (BW) and calf size with placental size. Expelled placentas and calf size at birth were collected from crossbred beef heifers and cows during four experiments (n = 22 to 39/experiment). Placentas deemed complete by visual inspection were dissected; dry weights were determined for cotyledonary and intercotyledonary tissues. Mixed linear models were used to individually determine main effects of peripartum BCS category [Thin (<5), Moderate (=5), or Fleshy (≥6)], age of dam category [Primiparous (2 yr), Young (3–4 yr), or Mature (≥5 yr)], and calf sex on placental measures. Correlations were determined for placental characteristics with prepartum dam BW, gestation length, and calf size. Thin BCS dams had lower (P ≤ 0.05) cotyledonary, total placental, and average cotyledon weights and greater placental efficiency (calf birth BW/placental weight) than moderate and fleshy dams. Intercotyledonary weight was lower (P < 0.01) in thin BCS dams compared with fleshy dams. Thin and moderate BCS dams had smaller (P ≤ 0.04) calf birth BW than fleshy dams. Primiparous dams had lower (P ≤ 0.05) total placental and average cotyledon weights than young and mature dams, yet calf birth BW was unaffected (P = 0.17). Male calves were heavier (P = 0.01) than females, yet there were no differences (P ≥ 0.59) in placental weights. Calf birth BW and heart girth had moderate positive correlations (P < 0.01) and shoulder to rump length and abdominal girth had weak positive correlations (P < 0.01) with all placental weights. Dam prepartum BW and calf flank girth had moderate positive correlations (P < 0.01) with total placental weights and weak positive correlations (P < 0.01) with cotyledonary and average cotyledon weights. Intercotyledonary weight had moderate positive correlations (P < 0.01) with gestation length and calf flank girth and a weak positive correlation (P < 0.01) with dam prepartum BW. Gestation length had a weak positive correlation (P = 0.02) with total placental weight. Number of cotyledons was not correlated (P ≥ 0.28) with any dam or offspring characteristics. In conclusion, these data suggest that both maternal age and BCS affected placental size. Calf size at birth and placental weight were positively correlated, but it is still unknown which controls and signals for the growth of the other.

71 Effect of follicle-stimulating hormone dose and circulating progesterone before ovum pickup and invitro embryo production in pregnant Holstein heifers

Superstimulation with exogenous FSH before ovum pickup for invitro embryo production (IVP) improves embryo production. However, there is limited information on different FSH dose regimens and the effect of progesterone (P4) concentrations on IVP. Thus, the objective of the present study was to evaluate the effect of FSH dose and circulating P4 on ovarian superstimulation responses, before ovum pickup (OPU) on IVP in pregnant heifers. Pregnant Holstein heifers (n=47) 19.5±0.3 months of age with moderate body condition score (3.3±0.03; scale 1 to 5) were randomly assigned to 2 treatment groups, p-FSH (Folltropin-V, Vetoquinol) 160mg (FSH160) or p-FSH 300mg (FSH300), in a crossover design. Two sessions of OPU were performed on each animal at 48 and 62 days of gestation, with a washout interval between sessions of 14 days. Dominant follicle removal (DFR) was utilised to synchronize follicular wave emergence, and FSH treatments were initiated 36h later. Heifers in both groups received a total of 4 FSH injections 12h apart as follows: FSH160 (48.0, 42.7, 37.3, and 32.0mg) or FSH300 (90.0, 80.0, 70.0, and 60.0mg). All heifers underwent OPU 40h after the last FSH treatment. Blood samples were collected for P4 quantitation at OPU and number of small (&amp;lt;6mm), medium (6–10mm), and large (&amp;gt;10mm) follicles were determined before OPU. Cumulus–oocyte complexes (COCs) recovered were processed and invitro embryo production was performed using standard procedures. Differences between treatment groups were evaluated using generalized linear mixed models, and data are presented as mean±s.e.m. (Table 1). Heifers treated with 300mg of p-FSH had a greater (P&amp;lt;0.05) number of medium-size follicles, total number of follicles, total COCs recovered, number of viable COCs, and number of blastocysts produced. In addition, heifers treated with 300mg of p-FSH had a greater cleavage rate (P=0.03). Circulating P4 was on average 10.1±0.4ng mL−1 and did not differ between gestation days (P=0.12). Furthermore, circulating P4 did not affect (P&amp;gt;0.10) any of the outcomes evaluated, nor was there an FSH dose by circulating P4 interaction (P&amp;gt;0.05). In conclusion, increasing the dose of p-FSH before OPU from 160 to 300mg resulted in an increase in the total number of follicles, number of oocytes, and number of blastocysts produced in pregnant donors without changes in blastocyst rate, regardless of progesterone concentrations at the time of OPU. Table 1. Ovum pickup and invitro embryo production in pregnant heifers treated with different doses of p-FSH Item FSH160 FSH300 P-value Small follicles (n) 5.0±0.6 3.5±0.4 0.02 Medium follicles (n) 14.4±0.8 18.3±0.9 0.0003 Large follicles (n) 2.9±0.3 2.5±0.3 0.28 Total follicles (n) 22.3±0.7 24.3±0.9 0.04 Total oocytes (n) 12.8±0.7 14.7±1.0 0.02 Recovery rate (%) 56.2±2.1 59.7±2.7 0.20 Viable oocytes (n) 11.4±0.7 13.3±1.0 0.02 Viable oocytes (%) 89.9±1.7 88.0±2.4 0.22 Cleavage rate (%) 63.8±4.3 71.5±3.7 0.03 Cleaved oocytes (n) 7.2±0.7 9.3±0.9 0.14 Blastocysts (n) 2.7±0.4 3.6±0.7 0.02 Blastocyst rate (%) 22.0±3.3 22.4±3.6 0.74

125 Influence of length of porcine follicle-stimulating hormone (p-FSH) treatment before ovum pickup on ovarian response and invitro embryo production in Holstein heifers

Ovarian follicle stimulation with exogenous FSH before ovum pickup (OPU) in Bos taurus females is a common practice to increase invitro embryo production (IVP). The optimal stimulatory period length for OPU-IVP, however, has not been definitively ascertained. The objective of the present study, therefore, was to determine the effect of length of the superstimulatory treatment period before OPU on ovarian response and IVP in Holstein heifers. Nonpregnant heifers (n=57) 13.8±0.2 months of age with moderate body condition score (3.0±0.1; scale 1 to 5) were assigned in a completely randomised design to one of the following experimental groups: FSH2d: 200mg of p-FSH (Folltropin-V®, Vetoquinol) distributed in four injections (60, 60, 40, and 40mg) of FSH 12h apart; FSH3d: 200mg of p-FSH distributed in six injections (40, 40, 40, 40, 20, and 20mg) of FSH 12h apart. Timing of follicular wave emergence was synchronized by dominant follicle removal 36h before the first p-FSH injection in all heifers. An intravaginal progesterone (P4) implant (1.38g of P4 CIDR®, Zoetis) was inserted at the time of the first p-FSH injection and removed at the time of OPU, which occurred in all heifers at 44h (Nivet et al. 2012 Reproduction 143, 165-171; https://doi.org/10.1530/REP-11-0391) after the last p-FSH injection. Additionally, follicle number was determined at OPU and classified as small (&amp;lt;6mm), medium (6–10mm) or large (&amp;gt;10mm). Oocytes from follicles of different sizes were pooled by heifer at OPU and then classified and subjected to IVP procedures. Differences between treatment groups were evaluated using generalized linear mixed models (SAS 9.4; SAS Institute Inc.) and data are presented as mean±s.e.m. (Table 1). Lengthening the FSH treatment period resulted in a greater (P=0.01) number of large follicles; however, the number of small, medium-size, and total follicles was not different between groups. Number of total recovered oocytes, viable oocytes, cleaved oocytes, as well as recovery rate, percent viable oocytes, and cleavage rate were not different (P&amp;gt;0.2) between groups. Similarly, the number of blastocysts produced per heifer and blastocyst rate were not different (P&amp;gt;0.9) among groups. In conclusion, lengthening the period of FSH treatment by 1 day increased the number of large follicles at OPU but did not improve overall ovarian response, oocyte recovery, or embryo production. Table 1. Ovarian response and IVP of heifers treated with p-FSH during 2 or 3 days before ovum pickup Response FSH2d (n=28) FSH3d (n=29) P-value Small follicles (n) 5.9±0.6 5.7±0.8 0.83 Medium follicles (n) 17.0±2.4 12.9±1.6 0.18 Large follicles (n) 2.5±0.5 4.5±0.6 0.01 Total follicles (n) 25.4±2.6 23.1±1.8 0.60 Total oocytes (n) 17.0±2.5 13.0±1.2 0.23 Recovery rate (%) 62.6±3.7 56.9±3.1 0.26 Viable oocytes (n) 14.4±2.0 11.3±1.1 0.30 Viable oocytes (%) 85.0±2.4 88.0±3.0 0.31 Cleaved oocytes (n) 8.7±1.6 7.1±1.2 0.62 Cleavage rate (%) 54.7±5.7 54.1±5.7 0.96 Blastocysts (n) 3.2±0.6 3.2±0.7 0.98 Blastocyst rate (%) 20.6±4.0 22.4±3.7 0.97

Cow- and herd-level factors associated with lameness in dairy farms in Peninsular Malaysia

Lameness is a major welfare issue in dairy cows. This study was aimed at investigating the cow- and herd –level factors associated with lameness in dairy farms from four states in Peninsular Malaysia. The study population was 1001 lactating cows from 28 dairy farms located in Selangor (n = 9), Perak (n = 8), Negeri Sembilan (n = 6) and Johor (n = 5). Lameness was assessed by locomotion scoring. Individual cow characteristics such as breeds, parity, body condition score (BCS), hock condition, leg hygiene, presence of claw lesion and claw overgrowth were recorded. Data on herd characteristics, management practices and housing design were collected by on-farm inspection and farmers’ interview. Mixed-eff ;ects logistic regressions were used to model the data and to assess the factors associated with lameness. Cow-level lameness prevalence was 34.2 % (95 % CI 22.2–50.0 %), with all the farms having at least one case of lameness. Claw lesions were recorded in 470 cows (46.9 %; CI 33.3–63.3 %) of which 296 (62.9 %) of them were lame. Of these, 78.9 % of the lesions were present on the rear feet and 25.5% of the cows had more than one foot affected. The proportion of cows having non-infectious and infectious claw lesions were 81.9 % and 18.1 %, and the predominant claw lesions were sole ulcers (24.9 %), white line disease (19.6 %), sole haemorrhage (10.2%), swelling of coronet (9.6 %), toe ulcers (8.4 %), and digital dermatitis (5.6%). Cows at third or more parities had higher odds of lameness (OR = 2.2; 95 % CI 1.2–4.1) compared to primiparous cows. Low BCS (< 2.5) increased the odds of lameness (OR = 4.8; 95 % CI 2.9–7.9) relative to cows with moderate BCS, and cows with hair loss around the hock (OR = 1.4; 95 % CI 1.1–1.9) relative to those with normal hock condition. Greater odds of being lame was observed in cows having claw lesion (OR = 15.2; 95 % CI 10.4–19.2) and those with overgrown claw (OR = 3.3; 95 % CI 2.4–4.5). There was increased odds of lameness in farms with high stocking density (OR = 1.8; 95 % CI 1.1−3.1), concrete floored walkways (OR = 1.9; 95 % CI 1.0–3.6), dirty floors (OR = 2.3; 95 % CI 1.9–3.7), and practicing preventive claw trimming (OR = 2.3; 95 % CI 1.9–4.6). Based on the high lameness prevalence, these findings could assist dairy farmers to make informed decisions on areas to implement on-farm changes to reduce lameness in the studied herds.

Associations between body condition score at parturition and microRNA profile in colostrum of dairy cows as evaluated by paired mapping programs

MicroRNA (miRNA) are abundant in milk, and likely have regulatory activity involving lactation and immunity. The objective of this study was to determine the miRNA profile in colostrum of overconditioned cows compared with cows of more moderate body condition score (BCS) at calving. Multiparous cows with either high (≥4.0 on a scale of 1 to 5; n = 7) or moderate BCS (2.75 to 3.50; n = 9) in the week before parturition were selected from a commercial dairy herd. Blood and colostrum were sampled within 24 h after calving. Blood serum was analyzed for free fatty acid (FFA) concentration. MicroRNA was isolated from colostrum samples after removing milk fat and cells. MicroRNA were sequenced, and reads were mapped to the bovine genome and to the existing database of miRNA at miRBase.org. Two programs, Oasis 2.0 and miRDeep2, were employed in parallel for read alignment, and analysis of miRNA count data was performed using DESeq2. Identification of differentially expressed miRNA from DESeq2 was not affected by the differences in miRNA detected by the 2 mapping programs. Most abundant miRNA included miR-30a, miR-148a, miR-181a, let-7f, miR-26a, miR-21, miR-22, and miR-92a. Large-scale shifts in miRNA profile were not observed; however, colostrum of cows with high BCS contained less miR-486, which has been linked with altered glucose metabolism. Colostrum from cows with elevated serum FFA contained less miR-885, which may be connected to hepatic function during the transition period. Potential functions of abundant miRNA suggest involvement in development and maintenance of cellular function in the mammary gland, with the additional possibility of influencing neonatal tissue and immune system development.

Forelimb Amputation and Long-Term Follow-Up in a Female Donkey

Background: Limb amputation may be a life-saving procedure for animals and minimally impact their comfort and quality of life, as previously reported in pets. This procedure is an appropriate alternative to euthanasia when catastrophic injury to a limb prevents its successful restoration. In horses, limb amputation has been performed for the past 40 years. Although in the reviewed literature there are no scientific reports of limb amputation in donkeys. This paper aimed to report a successful forelimb amputation and long-term follow-up in a female donkey.Case: A 10-month-old and 95 kg female Nordestino donkey was evaluated for a wound with bone exposition on the left forelimb. Physical examination revealed that the donkey was alert with moderate body condition score, tachycardia, tachypnea, and a medial wound revealing the necrotic aspect of the left radius. Radiographic examination presented Salter-Harris type 1 exposed radius fracture. Hematology revealed normocytic and normochromic anemia, and hyperfibrinogenemia. Due to the catastrophic injury and no forecast for building a prosthesis, the donkey underwent general anesthesia for left forelimb amputation through the midhumerus diaphysis. A fish-mouth skin incision was performed on the midhumerus, the underlying musculature was dissected and the vessels ligated until the bone could be accessed. Then, a sterile saw wire was used to transect it, and bone edges were rounded. Muscle and fascia were harvested with the skin to provide additional padding at the end of the stump. Postoperatively, the donkey was submitted to broad spectrum antibiotic, anti-inflammatory and analgesic therapy, and tetanus prophylaxis. Preventative treatment for overload laminitis included frog support pads and resting leagues on all three legs. No short-term complications were observed and the donkey made a full recovery. Long-term complications included fistula formation on the stump, and distal interphalangeal joint hyperflexion, probably by weight overload causing acquired deep digital tendon contracture on the right forelimb. Currently, eight years (96-months) past surgery, the donkey is still well adapted and has a good quality of life even without a limb prosthesis.Discussion: Traditionally, catastrophic leg injury in equids often requires euthanasia. Currently, limb amputation is becoming more accepted by owners and a viable life salvage option. In horses, limb amputation by disarticulation using a caudal flap technique is the most usual surgical technique, but presents some chronic complications, such as osteomyelitis of the stump, pressure sores from the prosthesis and contralateral limb failure. Although not commonly performed in equids, limb amputation through the midhumerus diaphysis was well accepted by the donkey. In horses, survival rate reached 50% and 32.5% at 18 and 24 months after amputation with prosthetic fitting, respectively. The donkey herein reported is still alive and presenting a good life quality at 96-months post-surgery, even without a forelimb prosthesis. This kind of feature has been only reported in a Samba deer followed-up for 10-months, showing good corporal condition, walking and even running at slow speed, using three legs. To the authors knowledge this is the first scientific report of limb amputation in a donkey. We emphasize that documenting more limb amputation in equids is essential to obtain conclusions about the prognosis, life expectancy and expected quality of life.

Factors affecting the semen quality of breeding bulls

This study aimed to investigate the effects of breed, age, scrotal circumference, Body Condition Score (BCS), season and nutrition on bull semen quality at breeding bull station of Ejab Alliance Limited, Thakurgaon, Bangladesh. A total of 777 ejaculates were collected from 25 bulls (Holstein-Friesian-10; Sahiwal-15). All semen parameters were significantly (P&lt;0.05) higher in Sahiwal bulls than in Holstein-Friesian. The volume, individual motility, mass activity and sperm morphology were significantly (P&lt;0.05) higher in bulls aged &gt;3.5 - 4.5 years than in younger (2.5 - 3.5 years) and older bulls (&gt;4.5 years). Scrotal circumference significantly (P&lt;0.05) affected all the parameters of semen quality. The highest values were observed in bulls with scrotal circumference 31.1 - 33.0 cm and the lowest in bulls with scrotal circumference of 33.1 cm and less. The volume, individual motility, mass activity and sperm morphology were higher (P&lt;0.05) in bulls with BCS of 4 to 4.5 than in bulls with BCS of &gt;4.5 to 5. Significantly (P&lt;0.05) highest values regarding semen quality were in winter and the lowest in summer. Vitamin ADE supplementation significantly (P&lt;0.05) improved the semen quality. It is suggested that Sahiwal breeds may be reared at the bull station in the subtropical climate of Bangladesh. Semen should be collected during winter in bulls of &gt;3.5 to 4.5 years of age with moderate scrotal circumference and BCS.&#x0D; The Bangladesh Veterinarian (2018) 35(1&amp;2): 32-39

Feeding dynamics and performance of Beetal does in relation to body condition score at the time of mating

The body condition of does at the time of mating may affect feeding patterns and production performance during forthcoming lactation. For investigation, 40 Beetal does available for breeding were utilized, and their body condition score (BCS) was estimated using the visual-cum-palpation method with a 9-point scale (scale of 1=5 with increments of 0.5 point). The available goats were divided into 3 groups: higher BCS (T ≥ 3.0 role=presentation> ≥ 3.0 ≥ 3.0 _{≥3.0} ; n =11), moderate BCS (T 2.5 role=presentation> 2.5 2.5 _{2.5} ; n = 8), and lower BCS (T ≤ 2.0 role=presentation> ≤ 2.0 ≤ 2.0 _{≤2.0} ; n = 21) and maintained in 2 random groups (20 each). Beetal goats with higher BCS at breeding time spent significantly (P ≤ 2.0 ≤ 2.0 _{≤2.0} goats struggled (displacement and pushing) more at feeders, which included T ≤ 2.0 role=presentation> ≤ 2.0 ≤ 2.0 _{≤2.0} goats eating from the ground for more time (P ≥ 3.0 ≥ 3.0 _{≥3.0} goats had more agonistic interactions (threatening and hitting) toward moderate and lower BCS goats at feeders. Goats with higher BCS at mating had significantly (P ≥ 3.0 ≥ 3.0 _{≥3.0} group than other groups. It was concluded that Beetal goats should not have less than 3.0 BCS at the time of mating for better performance and health status.

Effects of close-up body condition score and selenium-vitamin E injection on lactation performance, blood metabolites, and oxidative status in high-producing dairy cows

High-producing dairy cows with high pre-calving body condition score (BCS) are more susceptible to metabolic disorders and oxidative stress. The aim of present study was to evaluate the effects of close-up BCS and 3 times Se-vitamin E (SeE) injection on BCS change, blood metabolites, oxidative status, and milk yield in high-producing Holstein cows. A total of 136 multiparous cows were divided into 2 groups based on their BCS including high (HB = 4.00 ± 0.20) and moderate (MB = 3.25 ± 0.25) at 3 wk before expected calving time. Then, each group was divided into 2 subgroups: 3 rounds of SeE injection at 21 d before, and 0 and 21 d after calving (+SeE), and no SeE injection (-SeE). Four final experimental groups were HB+SeE, MB+SeE, HB-SeE, and MB-SeE (34 cows each). Results indicated that interaction effect of BCS and SeE affected serum glucose, and the MB+SeE group had the highest level. The HB cows lost more BCS compared with MB cows during the postcalving period. Moreover, serum insulin concentration increased after SeE injection. The HB cows had higher serum nonesterified fatty acids at 14 d after calving. The MB cows tended to have higher activity of blood glutathione peroxidase over the study period. Furthermore, the SeE-injected cows tended to have higher activity of blood glutathione peroxidase at 28 d after calving. Serum albumin level was increased by SeE injection. The HB cows had greater milk production than MB cows, and SeE-injected cows tended to have higher milk fat percentage and higher fat:protein ratio compared with nonsupplemented cows. It was concluded that SeE injection had beneficial effects on some blood metabolites, albumin as a blood antioxidative parameter, and lactation performance in high-producing dairy cows, especially cows with moderate close-up BCS.

Body Condition Scores at Calving and Their Association with Dairy Cow Performance and Health in Semiarid Environment Under Two Cooling Systems

Under tropical environments cow’s body condition at calving may influence productivity and health performance. Therefore, the current research evaluated the impact of body condition score (BCS) at calving on milk production traits and health problems of primiparous and multiparous cows raised under two cooling systems in semi-arid environment. Milk yield parameters and health disorders data at different parities were obtained from 1700 Holstein cows, which were managed under Korral Kool (KKC) and fan (FC) cooling systems in Saudi Arabia. The results revealed that KKC system induced high level of milk production improvement (305TM, days in milk, daily milk yield) (P<0.05) compared to FC, especially in multiparous cows. There was an advantage of the high BCS group under KKC system over the FC system for peak yield and period. Cows from both cooling systems having moderate BCS outdid the other groups for total milk yield. Low BCS primiparous cows reared under KKC system were the most to suffer from stillbirth (18%), and calving ease (26.1%) in winter, while high BCS were the most to be treated for mastitis (8.5% in summer). On the other hand, spring calved multiparous cows raised under FC system suffered more from lameness (21.57% - high BCS) and milk fever (4.4% - moderate BCS). Further, cows having moderate BCS kept under KKC system had the highest incidence of lameness (62.2%), and abomasum displacement (4.4%). In conclusion, achieving correct BCS at calving and cooling system is important to avoid calving subsequent lactation performance and metabolic disease losses.

Non-invasive indicators associated with the milk yield response after anthelmintic treatment at calving in dairy cows.

BackgroundGastrointestinal nematodes are an important cause of reduced performance in cattle. Previous studies in Europe showed that after anthelmintic treatment an average gain in milk production of around 1 kg per day/cow can be expected. However, (1) these studies have mainly evaluated group-based anthelmintic treatments during the grazing season or at housing and (2) little is known about parameters affecting variations in the treatment response amongst cows. A better knowledge of such parameters could help to select animals that benefit most from treatment and thus lead to a more rational use of anthelmintics. Therefore, a randomized, non-blinded, controlled clinical trial was performed on 11 commercial dairy farms (477 animals) in Belgium, aiming (1) to study the effect of eprinomectin treatment at calving on milk production and (2) to investigate whether the milk yield response was related to non-invasive animal parameters such that these could be used to inform targeted selective treatment decisions.ResultsAnalyses show that eprinomectin treatment around calving resulted in an average (± standard error) increase of 0.97 (±0.41) kg in daily milk yield that was followed up over 274 days on average. Milk yield responses were higher in multiparous compared to primiparous cows and in cows with a high (4th quartile) anti-O. ostertagi antibody level in a milk sample from the previous lactation. Nonetheless, high responses were also seen in animals with a low (1st quartile) anti-O. ostertagi antibody level. In addition, positive treatment responses were associated with higher faecal egg counts and a moderate body condition score at calving (2nd quartile).ConclusionsIn conclusion, this study provides novel insights into the production response after anthelmintic treatment at calving and factors which influence this. The data could be used to support the development of evidence-based targeted selective anthelmintic treatment strategies in dairy cattle.

Non-invasive indicators associated with the milk yield response after anthelmintic treatment at calving in dairy cows

Gastrointestinal nematodes are an important cause of reduced performance in cattle. Previous studies in Europe showed that after anthelmintic treatment an average gain in milk production of around 1 kg per day/cow can be expected. However, (1) these studies have mainly evaluated group-based anthelmintic treatments during the grazing season or at housing and (2) little is known about parameters affecting variations in the treatment response amongst cows. A better knowledge of such parameters could help to select animals that benefit most from treatment and thus lead to a more rational use of anthelmintics. Therefore, a randomized, non-blinded, controlled clinical trial was performed on 11 commercial dairy farms (477 animals) in Belgium, aiming (1) to study the effect of eprinomectin treatment at calving on milk production and (2) to investigate whether the milk yield response was related to non-invasive animal parameters such that these could be used to inform targeted selective treatment decisions. Analyses show that eprinomectin treatment around calving resulted in an average (± standard error) increase of 0.97 (±0.41) kg in daily milk yield that was followed up over 274 days on average. Milk yield responses were higher in multiparous compared to primiparous cows and in cows with a high (4th quartile) anti-O. ostertagi antibody level in a milk sample from the previous lactation. Nonetheless, high responses were also seen in animals with a low (1st quartile) anti-O. ostertagi antibody level. In addition, positive treatment responses were associated with higher faecal egg counts and a moderate body condition score at calving (2nd quartile). In conclusion, this study provides novel insights into the production response after anthelmintic treatment at calving and factors which influence this. The data could be used to support the development of evidence-based targeted selective anthelmintic treatment strategies in dairy cattle.

Metabolic and endocrine profiles of primiparous beef cows grazing native grassland. 2. Effects of body condition score at calving, type of suckling restriction and flushing on plasmatic and productive parameters

The objective of the present experiment was to analyse the effect of body condition score (BCS) at calving, type of suckling restriction and flushing on metabolic and endocrine profiles, and productive and reproductive responses of primiparous beef cows grazing native grassland. Primiparous beef (n = 56) cows in anestrus classified by BCS at calving (low ≤3.5 and moderate ≥4; 1–8 visual scale) were assigned randomly to four treatments in a two by two factorial arrangement of type of suckling restriction and flushing. Type of suckling restriction started at 55 ± 10 days postpartum (DPP ± s.e.m.; Day 0 = initiation of the treatment) and consisted of applying nose plates to calves for 12 days (i.e. TS treatment) or 5 days of isolation of the cow–calf pair, followed by applying nose plates to calves for 7 days as calves were reunited with their mothers (i.e. IS treatment). Immediately after the suckling restriction treatments were finished, the breeding season started, and each cow received (flushing group) or not (control group) 2 kg/day (fresh basis) of whole-rice middling for 22 days. The BCS was superior in moderate-BCS cows through the experiment. The type of suckling restriction did not affect any plasma parameter, but insulin-like growth factor I (IGF-I) concentrations increased in all cows during suckling restriction. Cholesterol concentration was affected by flushing × day interaction (P &lt; 0.05), while insulin and IGF-I concentrations were affected by the interaction among BCS at calving, flushing and days (P &lt; 0.03). Flushing increased cholesterol concentration in both BCS groups at calving, while insulin and IGF-I concentrations increased during flushing only in moderate-BCS cows. Suckling restriction, flushing, and BCS at calving did not affect calf weight or milk production. Moderate BCS cows had a shorter postpartum anestrous interval (PPI) (98 vs 123 DPP; P &lt; 0.01). Isolated type of suckling reduced postpartum anestrous interval when compared with TS treatment (97 vs 115 DPP, P &lt; 0.05). Early pregnancy rate was greater in flushed than in control cows (0.8 vs 0.55, P &lt; 0.01) and in moderate-BCS cows than in low-BCS cows (0.84 vs 0.46, P &lt; 0.01). Total pregnancy rate was also greater in flushed and moderate cows and tended to be affected by the interaction between flushing and BCS at calving (P = 0.06; flushed cows: moderate = 1 vs low = 0.5, P &lt; 0.08; and control cows: moderate = 0.8 vs low = 0.4, P &lt; 0.09). These results confirmed the great value of suckling restriction and flushing during post-calving and relevance of BCS at calving as a link between energetic nutrition and metabolic and reproductive processes in primiparous beef cows grazing native grassland.

Breed differences in insulin sensitivity and insulinemic responses to oral glucose in horses and ponies of moderate body condition score

Breed-related differences may occur in the innate insulin sensitivity (SI) of horses and ponies, an important factor believed to be associated with the risk of laminitis. The aim of this study was to measure the glucose and insulin responses of different breeds of horses and ponies in moderate body condition to a glucose-containing meal and to compare these responses with the indices of SI as determined by a frequently sampled intravenous glucose tolerance test (FSIGT). Eight Standardbred horses, 8 mixed-breed ponies, and 7 Andalusian-cross horses with a mean ± SEM BCS 5.0 ± 0.3 of 9 were used in this study. Each animal underwent an oral glucose tolerance test (OGTT) in which they were fed a fiber-based ration (2.0 g/kg BW) containing 1.5 g/kg BW added glucose, as well as a standard FSIGT with minimal model analysis. The glucose response variables from the OGTT were similar between groups; however, the peak insulin concentration was higher in ponies (94.1 ± 29.1 μIU/mL; P = 0.003) and Andalusians (85.3 ± 18.6; P = 0.004) than in Standardbreds (21.2 ± 3.5). The insulin area under the curve was also higher in ponies (13.5 ± 3.6 IU·min·L−1; P = 0.009) and Andalusians (15.0 ± 2.7; P = 0.004) than in Standardbreds (3.1 ± 0.6). Insulin sensitivity, as determined by the FSIGT, was lower in Andalusians (0.99 ± 0.18 × 10−4/[mIU·min]) than in Standardbreds (5.43 ± 0.94; P < 0.001) and in ponies (2.12 ± 0.44; P = 0.003) than in Standardbreds. Peak insulin concentrations from the OGTT were negatively correlated with SI (P < 0.001; rs = −0.75). These results indicate that there are clear breed-related differences in the insulin responses of horses and ponies to oral and intravenous glucose. All animals were in moderate body condition, indicating that breed-related differences in insulin dynamics occurred independent of obesity.

229 INFLUENCE OF HIGH OR LOW INTAKE OF DRY MATTER AND ENERGY ON IN VIVO PRODUCTION OF BOVINE EMBRYOS

The aim of this study was to evaluate the influence of high or low dry matter (DM) intake and/or energy on embryo quality and production in vivo. Nonlactating Nelore cows (n = 32, 4 to 10 years old) weighing 489.5 ± 11.3 kg and with a body condition score of 3.25 (1 to 5) were used. After 15 days on the adaptation diet, cows were blocked by initial body weight (BW) and randomly divided in 4 experimental groups. The maintenance group (M) received a diet to provide 1.2% of DM/kg of BW. The restriction group (0.7M) received the equivalent of 70% of the group M diet (0.84% of DM per kg of BW). The high intake group (1.5M) received the equivalent of 150% of the M group (1.8% of DM/kg of BW). The energy group (E) received a diet with a DM similar to the M group but with an energy level equivalent to the 1.5 M group. All cows were submitted to aspiration of follicles &gt;2 mm for ovum pick-up (OPU). Recovered oocytes were used in another experiment. The embryo donors received an intravaginal device (IVD) of progesterone release (Sincrogest®, Ouro Fino, Ribeirão Preto, Brazil), soon after OPU. Two days after OPU, the cows received 8 decreasing doses of FSH (100 mg, IM, Folltropin-V®, Bioniche Animal Health, Belleville, Ontario, Canada) and concomitant with the fifth and sixth treatments of FSH, PGF2α (500 μg each, IM, cloprostenol, Sincrocio®, Ouro Fino) was administered. The IVD was removed at the time of the last FSH injection. Twelve hours after IVD removal ovulation was induced with GnRH (0.01 mg, IM, Buserelin acetate, Sincroforte®, Ouro Fino). Cows were inseminated 12 and 24 h later. Embryo collection was performed 7 or 8 days after GnRH injection. The cows were offered all diets in a crossover design study. There were 4 sessions of embryos flushing, each 42 days apart. Data were analysed by PROC GLIMMIX of SAS (SAS Institute Inc., Cary, NC, USA) and the results are presented as least-squares means ± SE, always following the order of treatments 0.7 M, M, 1.5 M and E. There were differences in the superstimulatory response (12.6 ± 1.4b; 14.6 ± 1.6a; 13.6 ± 1.5ab; and 11.0 ± 1.2c follicles &gt;6 mm; P &lt; 0.01) and superovulatory response (9.8 ± 1.3ab; 11.0 ± 1.4a; 10.2 ± 1.3a; and 8.6 ± 1.3b CL; P &lt; 0.01) among groups. Despite the lower responses observed especially in the high-energy group, no difference among groups was observed for total embryos/ova (4.5 ± 0.7; 5.0 ± 0.8; 5.0 ± 0.8; and 4.7 ± 0.7; P = 0.60), viable embryos (2.0 ± 0.4; 2.3 ± 0.5; 2.6 ± 0.6; and 2.2 ± 0.5; P = 0.40), or freezable embryos (1.7 ± 0.4; 2.0 ± 0.4; 2.1 ± 0.5; and 1.9 ± 0.4; P = 0.60). There was also no difference among groups for fertilization rate (75.8 ± 9.6; 82.3 ± 8.0; 87.8 ± 6.6; and 81.1 ± 8.4%; P = 0.71) and percentage of viable embryos (54.5 ± 10.8; 50.8 ± 10.6; 50.5 ± 10.9; and 54.4 ± 10.7%; P = 0.98). In conclusion, in contrast to our initial hypothesis, a period of 42 days under high feed/energy intake in nonlactating zebu cows apparently did not compromise in vivo embryo production. This may be because cows had a moderate body condition score or because the feeding period was not long enough to compromise oocyte/embryo quality. Financial support from FAPESP and CNPq is acknowledged.

Effect of short period feed supplementation during early lactation on performance of cows and calves raised in extensive system

Thirty-two multiparous Angus cows at moderate body condition score (3 in a 1-5 scale) received feed supplementation at the beginning of lactation for a short time to determine the effect on milk yield and composition and on performance of cows and calves. Supplementation started on days 22 ± 3.2 after calving. The cows were randomly distributed into two groups (n =16), one group receiving feed supplementation for 35 days (supplemented group), and the other without supplementation (control group). Each cow received 0.6% of its body weight in individual troughs daily. Both groups were kept in native pasture condition during supplementation period. Milk yield evaluation, performance of cows and calves were controlled on day 0 (initial), day 14 (middle) and day 35 (final) of the supplementation period. There was no effect of feed supplementation on milk yield or on any other milk component in both groups, and on calf performance as well. Milk composition differed among periods, when fat was the highest in the middle of supplementation and lactose was the highest in the end of the period. Supplemented cows presented more daily average weight gain at the final period of supplementation than cows from the control group. Beef cow with moderate body condition score can gain weight during the lactation period as a response to increase of energy availability in a short period without any influence on milk production and composition as well as a consequence on calf performance.