Development Of Research Model Research Articles

Highly efficient construction of monkey blastoid capsules from aged somatic cells

Blastoids—blastocyst-like structures created in vitro—emerge as a valuable model for early embryonic development research. Non-human primates stem cell-derived blastoids are an ethically viable alternative to human counterparts, yet the low formation efficiency of monkey blastoid cavities, typically below 30%, has limited their utility. Prior research has predominantly utilized embryonic stem cells. In this work, we demonstrate the efficient generation of blastoids from induced pluripotent stem cells and somatic cell nuclear transfer embryonic stem cells derived from aged monkeys, achieving an 80% formation efficiency. We also introduce a hydrogel-based microfluidics platform for the scalable and reproducible production of size-adjustable, biodegradable blastoid capsules, providing a stable 3D structure and mechanical protection. This advancement in the high-efficiency, scalable production of monkey blastoid capsules from reprogrammed aged somatic cells significantly enhances the study of embryonic development and holds promise for regenerative medicine.

Pengembangan Video Pembelajaran Pada Materi Sistem Sirkulasi Tingkat SMA/MA

This study was conducted to produce learning media in the form of learning videos used by teachers in learning activities. Teachers use learning media in the form of videos taken from YouTube. Because teachers do not have their own learning videos designed. Videos taken from YouTube also do not support the material delivered because the content of the material from the videos taken from YouTube is not in accordance with the learning objectives, namely the video does not describe the latest material that is in accordance with the student's handbook, as a result students find it difficult to understand the contents of the videos delivered by the teacher. The use of available learning media is very limited during the learning process. The media used during learning is still infocus. Therefore, other learning resources are needed to help students understand. One effort to facilitate the learning process is by having media in the form of learning videos. The purpose of this study was to determine the development of learning media in the form of learning videos on the material of the circulatory system for SMA/MA class XI which is valid and practical. The type of research used is development research with a 4D development research model (Define, Design, Development, Disseminate). This research was carried out until the development stage, namely the validity test and practicality test. The results of the validity test showed that this learning video was in the very valid criteria with a validity value of 91%. The results of the practicality test by the teacher showed a value of 81% with practical criteria and the results of the practicality test of the learning video by students showed a value of 86% with very practical criteria. It can be concluded that the learning video on the material of the circulatory system for the SMA/MA level that was developed is valid and practical.

Influencial factors of domestic tourists‘ intention to choose wellness-tourism destinations in Vietnam

Purpose: Determine the impact of influencial factors on the intention to choose wellness tourism destination in Vietnam of domestic tourists. Method: The research used mixed methods which include quantity and quality methods. Desk research was firstly used to synthesize research background and develop research model. After that, a survey was implemented to collect data to test the hypothesis. Finally, focus group discussion was used to collect opinions of policy makers, tourism specialists on solutions to enhance domestic tourists’ intention to choose wellness tourism destination in Vietnam. Result: The result of the study shows that there are 4 factors affects the intention of domestic tourists’ in choosing wellness tourism destination in Vietnam: Attitude, subjective norms, perceived behavioral control and destination image. In which perceived behavioral control has the strongest impact and destination image is the only factor that has negative impact. Some solutions have been proposed to to optimize the impact factors, contributing to the promotion of sustainable development of wellness tourism in Vietnam. Contribution: The study makes contribution to clarifying the factors influencing domestic tourists' intention to choose wellness-tourism destinations in Vietnam which are attitude, subjective norms, perceived behavioral control and destination image. Based on the analysis results, some practical solutions to enhance domestic tourists' intention to choose wellness tourism destination in Vietnam were proposed: raising awareness of behavioral control by providing detailed information, developing flexible service packages, and improving infrastructure so that tourists feel autonomous and comfortable when deciding; enhancing subjective norms by encouraging sharing of travel experiences on social networks, cooperating with KOLs, and organizing group travel programs to take advantage of community influence; improving tourists' attitudes by diversifying and improving the quality of tourism products, building a positive environment at the destination, and promoting the benefits of wellness tourism; and improving the destination's image through strong communication campaigns, developing unique experience programs, and cooperating with international organizations to certify quality, create prestige, and attract tourists.

Integrated Project-Based Learning (PjBL) Learning Tools STEM: Improving Students' Creative Thinking Skills

Integrated STEM-based PjBL learning devices are a learning context designed by teachers to motivate students to take part in learning activities so they can boost their creative thinking skills and discover new ideas. The goal of this study was to examine a learning tool that combines STEM with Project-Based Learning. It looks at whether this tool is suitable, effective, and easy to use in order to help improve creative thinking skills of fourth-grade students of Santo Paulus Martubung Elementary School - Medan. This type of research uses the Research and Development (R&D) development model. The results of the study revealed that the devices developed had significantly improved students' creative thinking skills. The increase in creative thinking skills can be seen from the average n-gain score of 0.49 (moderate increase category). The learning devices developed on the 'Care for the Environment' material are effective in significantly improving students' scientific literacy. The increase in scientific literacy can be seen from the average score of 0.64 (moderate increase category) and increased interest in science and technology, active participation in discovery activities (practice and projects), and responsible management of natural resources and the the area around us after using a project-based learning tool along with a specific method.

Development of Guided Writing Teaching Materials Based on High Order Thinking Skills for Grade VI Elementary School Students

This study aims to develop guided writing teaching materials based on High Order Thinking Skills (HOTS) with the 4-D development research model approach (Define, Design, Develop, Disseminate). The development process begins with the define stage to identify student needs and analyze the curriculum, followed by the design stage to design the initial teaching materials. At the develop stage, the teaching materials were validated by media experts and material experts, and tested on 28 students of grade VI state elementary school 01 Wates Semarang. The last stage, disseminate, was conducted to disseminate the product openly to teachers and students in the context of learning. The results showed that validation from media experts resulted in an average score of 90.5% (very feasible category), while validation from material experts resulted in an average score of 92% (very feasible category). The trial of the developed teaching materials to 28 elementary school students of grade VI state elementary school 01 Wates in Semarang City and the results of the comparison between pretest and posttest were -4.543, with a p value (Asymp. Sig.) of 0.000 (p < 0.05) with an effective category of improving students' writing skills by providing stimulation to think critically, analytically, and creatively according to HOTS characteristics. Thus, the development of HOTS-based writing teaching materials can be an effective innovation in literacy learning in elementary schools, especially to improve in-depth and meaningful guided writing skills. This study recommends wider implementation of this teaching material in other classes as well as further development based on digital technology to reach a wider audience.

TIKTOK-BASED VIDEO TUTORIAL LEARNING MEDIA ON THE TOPIC OF SIMPLE 3D DRAWING WITH CAD

This research aims to produce a product in the form of a TikTok-based video tutorial learning media on the topic of basic 3D drawing command functions with CAD, and to determine the feasibility and user response towards this media. The research employs the Design Based Research (DBR) development model, which consists of four stages: problem identification, prototype solution development, testing and solution improvement, and reflection and solution implementation. The testing was conducted in class XI- DGM 1 in the subject of designing techniques with CAM, with a sample size of 35 students. The instrument used was a questionnaire. The research yields a video tutorial learning media based on the TikTok application for basic 3D drawing command functions with CAD. The feasibility assessment conducted by 3 subject-matter experts and 3 media experts indicates that the developed TikTok-based video tutorial learning media is deemed "very feasible." After being approved by the subject-matter and media experts, further testing was carried out in class XI-DGM 1 in the subject of designing techniques with CAM, using a questionnaire as the instrument. The user response results were categorized as "very positive." In conclusion, based on the research findings, this TikTok-based video tutorial learning media is highly suitable for use in teaching designing techniques with CAM.

Pengembangan E-LKPD Berbasis Somatic Auditory Visualizyion Intellectualy Menggunakan Live worksheet Pada Materi Suhu dan Kalor

In learning physics, students are less enthusiastic and less active when studying physics. This is because the teaching materials used by students are not in accordance with the students' learning styles and the LKPD used by educators during the learning process does not attract students' interest in learning because it only displays pictures and questions. The aim of this research is to Develop an E-LKPD based on Somatic Auditory Visualization Intellectualy using live worksheets on heat material in class XI SMA that is valid and practical. This type of research is 4D model development research which has four stages, namely: Define, Design, Development and Disseminate. The subjects in this research were 33 educators and students in class XI of the UNP Laboratory Development High School, Padang City. The instruments used in this research were a validity questionnaire and a practicality questionnaire. Then the data that has been obtained is analyzed to see the validity and practicality of the teaching materials. Based on the results of data analysis, it was found that the E-LKPD based on Somatic Auditory Visualization Intellectualy using live worksheets developed was categorized as very valid with an overall average of 86.5%. Apart from that, teaching materials are categorized as very partial with an overall average of 94.16%

Tri Hita Karana-based interactive e-worksheet for Indonesian language subjects to improve literacy skills and prevent bullying in elementary schools

Low literacy skills and the still high number of bullying cases are two problems currently faced in schools in Indonesia. Therefore, this study aims to develop an Interactive E-Worksheet Based on Tri Hita Karana for Indonesian Language Subjects to Improve Literacy and Prevent Bullying in Elementary Schools. To achieve these goals, this study was conducted by following the development research model with the ADDIE approach consisting of five stages: analysis, design, development, implementation, and evaluation. Data in this study were collected through observation, questionnaires and tests. Data were analyzed qualitatively and quantitatively. The results of this study indicate that the developed e-worksheet is proven valid and has a good level of practicality. In addition, based on the results of the analysis using MANOVA, it was found that learning using the Interactive E-Worksheet Based on Tri Hita Karana for Indonesian Language Subjects can effectively improve students' literacy skills and understanding of bullying so that bullying prevention can be carried out optimally. Thus, the practical implication of this study is that the Tri Hita Karana-based Interactive E-Worksheet for Indonesian Language Subjects, developed to improve literacy skills and prevent bullying, can be used as teaching materials to achieve similar goals in other schools.

Developing Recognizing Ability of Hijaiyah Letters Through the Hijaiyah Letter Tree Educational Game Tool at RA Asyarif Sibanggor Jae

The hijaiyah letter tree educational game tool is an effective and fun game for children. The ability to recognize hijaiyah letters at RA A-Syarif Sibanggor Jaeini is still relatively low. This research aims to find out: 1) how to make educational game tools. Educational game tools. Hijaiyah tree game. To recognize hijaiyah letters for young children at RA As Syarif. To find out the effectiveness of the Hijaiyah Tree Educational Game tool for early childhood children at RA As Syarif; 2) Use of educational game tools: Hijaiyah tree game to recognize hijaiyah letters for children at RA As Syarif. This research uses the development research (R & D) development model ADDIE (Analysis-Design-Develop-Implement-Evaluate) developed by Dickand Carey. Data collection in this research was by means of validator questionnaires, observation sheets, interviews, documentation. The results of this research obtained an average value for product validation by media experts, namely 3.42, adjusted to the scale 4 convection table, which is in the range 3.25 < M ≤ 4.00 in the “good” category. The assessment by material experts received an average product validation by material experts, namely 3.42, adjusted to the scale 5 convection table in the range 3.25 < M ≤ 5.00 in the “Very good” category. So it can be seen that the overall average is 4.55 from each aspect of the development assessment. It can be concluded that this hijaiyah tree educational game tool is declared “Decent”. The children’s responses were very positive, showing high enthusiasm for the learning media used. The average rating from teachers reached 5.00, which is in the Very Good category. Based on the results of validation and trials, it can be concluded that the Hijaiyah Letter Tree learning media product is suitable for use and has good effectiveness in improving children’s ability to recognize Hijaiyah letters, thus the Hijaiyah Letter Tree Game Tool for learning to recognize Hijaiyah letters is declared valid and does not need to be revised.

Solar cell based power-bank prototype as a media to increase students' scientific literacy

This research aims to i) implement the solar cell based power-bank prototype for learning media; ii) describe the feasibility of the solar cell-based power-bank prototype for learning; and iii) describe students' scientific literacy. This research method uses the analysis, design, development, implementation, and evaluation (ADDIE) research and development model. The subjects of this research are class IX students and science teachers at SMPN 4 Central Bengkulu. Data collection techniques include questionnaires, tests, and interviews. The data analysis technique uses N-gain analysis, percentages, and the Aiken's V formula. The research results show that the media developed is valid with an Aiken index value of 0.98 > the minimum standard Aiken' V of 0.92 and can increase students' scientific literacy based on an N-gain of 0.92. 0.71, this can be seen in the dimensions of context and content based on N-gain of 0.73 and 0.69, while the dimensions of attitude, curiosity, respect, and willingness, reflect the respective percentages of 79.62%, 78.86%, 78.89%, 75.60%. Based on the results of the hypothesis test, the sig value is obtained. (2 tailed) 0.000 < 0.05 which means Ha is accepted and Ho is rejected, which means the solar cell-based renewable energy media used in science learning influences the increase in students' scientific literacy.

Pengaruh Implementasi Media Pembelajaran Tas Ajaib Dalam Upaya Mengenal Identitas Diri Dan Keluarga Pada Siswa Kelas 1 SD Islam Al-Mubarok Surabaya

The present study aims to promote self-knowledge and familial awareness among children, thereby facilitating their socialization. The objective is threefold: first, to enhance students' interest and motivation in the learning process; second, to assist them in recognizing their own selves; third, to encourage interaction and collaboration among students; and fourth, to provide a learning experience that is both educational and enjoyable. Conventional learning media is often regarded as less appealing to early childhood students. This study proposes the utilization of "Magic Bag" media as an interactive and innovative alternative. The "Magic Bag of Self and Family Identity" media is hypothesized to facilitate the implementation of the self-introduction process for grade 1 students at Al-Mubarok Islamic Elementary School in an effective, engaging, and memorable manner, thereby fostering the development of crucial social and emotional competencies. The findings of the research are as follows: Firstly, the development of the conventional-based "Magic Bag" learning media on self-identity and family material is systematic and structured in accordance with the stages of the development research model. Secondly, the conventional-based "Magic Bag" learning media is declared very valid with an average percentage level The material validation percentage was found to be 90.76%, while the media validation percentage was 92.30%. Furthermore, the "Magic Bag" learning media has been determined to be highly effective, with a student learning completeness percentage of 98.33% in a limited trial.

PENGEMBANGAN MODEL LINTASKUJITU DALAM PEMBELAJARAN MENYIMAK FABEL

Listening learning haven’t received attention in planning lessons. Therefore, a model is needed that can apply listening principles in learning. This study developed the Lintaskujitu model for fables listening learning and described the results of the validation test and students' perceptions of the model implementation. The development research model used is 4D (define; design; development; disseminate). The expert of model and listening learning, and teacher were involved to validity test and 28 students became participants in implementation of Lintaskujitu model used quesionner. This research and development resulted in six stages of learning which are packaged in a guidebook. The Lintaskujitu model was declared feasible for implementation by experts and teacher with a percentage of 76% (the model concept), 79% (the model steps), and 75% (the level of student needs). Based on the results of this research and development, the Lintaskujitu model can be implemented in listening learning, especially in learning fables.

Role playing as contextual learning to improve mathematical communication skills of madrasah ibtidaiyah students

Among the efforts to improve Mathematical Communication Skills (MCS) is to innovate learning models. This research aims to develop contextual role-playing learning products to improve Madrasah Ibtidaiyah (MI/ primary school) students’ MCS on valid and practical fraction material. The research and development (R&D) model of Define, Design, Development, and Dissemination (4D) was employed. The research subjects were second graders at MI Tahfidz El Muna Q, totaling 28 students. The instruments included validation guidelines, practicality guidelines, and MCS tests. Product effectiveness is tested using N-Gain. The results showed that the number of students completing the MCS after contextual role-playing learning was 89.28%, which is in the high category. The average N-Gain value obtained was 0.79, which is also in the high category. These findings show that contextual role-playing learning can potentially improve MI students’ MCS, especially in fraction material.

Development of a Textbook on the History of Economic Thought with a Combination of Barcode Video in the Application of Differentiated Learning

Objectives: This research is to facilitate the diversity of student conditions in learning and can even be used simply for students with special needs at a low level such as deaf students and other limitations that can still be facilitated with a combination of audio visual media. Method: The type of research method used in this study is a research and development design . Reaserch and development is a research method used to produce a certain product and test the effectiveness of the product. The development model used in this study is a development research model by proposing a series of stages that must be taken in this approach, including research and information collecting, planning, developing preliminary form of product, preliminary field testing, main product revision, main field testing, operational product revision, operational field testing, final product revision, and dissemination and implementation. Results: Based on the results of validation that have been carried out by experts in media, language, and materials, as well as in limited trials, this book is considered very good and worthy of application in learning. The integration of technology in this textbook reflects significant developments in the development of interactive and digital educational resources. Novelty: A combination of video barcodes in diverse learning contexts This innovative approach combines digital technology with traditional teaching materials, so students can choose the most effective way of learning according to their learning style. Either visually, auditory, or kinesthetic. Thus, the ADDIE model is used to develop textbooks in getting positive assessments from each expert and limited trials. This shows that the approach is not only innovative, but also effective and suitable for application in the field of education.

Project-Based Learning Tool Model that empowers the Metacognitive Skills of MAN Model Manado Students

Problems in learning that do not empower students' thinking abilities, especially metacognitive skills, are of high concern in the 21st century. The demands of life require humans to have some required skills and must be empowered to learn through appropriate learning tools. This research aims to develop project-style learning tools that enable students' metacognitive skills in biology subjects. The research method used is Borg & Gall model development research with the following steps: 1) needs analysis to obtain the required information, 2) literature study, 3) development of learning tools using a project model, and 4) expert validation, then continued with limited group testing on users. The results of the development in the form of ATP and the Phase E Biology Teaching Module, show that the learning tools that have been developed are suitable according to material experts and learning experts with very high assessment categories, namely 94% and 90.66%, 100% of teacher assessment results, so that the learning tools are suitable for use. This is also reinforced by data from the results of applying learning tools to students by measuring students' metacognitive skills reaching a score of 76 or the good category. Keywords: learning tools, PjBL, metacognitive skills

Bibliometric Analysis of Spline Regression Model for Trend Mapping and Strategy Development Research Using Vosviewer

Bibliometric analysis is a quantitative method used to measure and analyze scientific literature. This technique involves the collection and analysis of publication data, such as journal articles, books, and conferences, to evaluate and understand patterns of scientific communication, research productivity, author collaboration, journal impact, and trends within a scientific field. Bibliometric analysis has a limitation in that it is challenging to visualize effectively, necessitating the use of software for proper visualization. Therefore, this article discusses bibliometric analysis on spline regression for trend mapping and strategy development using VOSviewer software. The research results show that the visualization of spline regression keywords with VOSviewer can help in understanding patterns of relationships between variables, research trends, and network structures in scientific literature. Based on the analysis results, bibliometric analysis on spline regression can be visualized in trend mapping, which can aid in planning further research strategies, including identifying collaboration opportunities and underexplored research areas.

Validity of Problem Based Learning Oriented E-LAPD for Practicing Creative Thinking Skills Reaction Rate Material

The era of education that occurs in the 21st century places demands on students to ensure the ability to think creatively to solve various problems. Apart from that, the development of the industrial revolution 4.0 plays a role in the current generation in terms of technology and the internet. The aim of this research is to determine the validity of E-LAPD oriented Problem Based Learning (PBL) to train creative thinking skills on reaction rate material. This research uses an R&D model, one of which is ADDIE. The ADDIE development research model consists of 5 stages, namely Analyze, Design, Develop, Implement, and Evaluate, but without carrying out the Implementation stage. This validation was carried out by 3 validators, namely 2 chemistry lecturers and 1 chemistry teacher at SMA Labschool Unesa 1 Surabaya. The results of the analysis of aspect of content validity get a mode value of 4 with a valid category, while for aspect construct validity it gets a mode value of 5 with a very valid category, the results of the content and construct validity values get a mode score of ≥ 3, so E- LPAD can be declared valid. So that E-LAPD media can be used in learning.

Characterisation of the novel SCN5A-P1891A variant, implicated in left ventricular noncompaction cardiomyopathy in a Finnish family, using human induced pluripotent stem cell-derived cardiomyocytes

Abstract Introduction Left ventricular noncompaction (LVNC) is a heterogenous cardiomyopathy characterised by an extensively trabeculated left ventricle (LV). Several genetic variants that perturb trabeculae compaction, and thus LV maturation, have been reported [1]. However, the precise mechanisms underpinning this have proven contentious with, for example, differentially aberrant cardiomyocyte proliferation implicated in the development of LVNC [2,3]. Purpose In this study, we investigate a novel SCN5A variant, P1891A, which is associated with LVNC in a Finnish family. We ascertain the effects of this variant through the characterisation of variant-carrying human induced pluripotent stem cell-derived cardiomyocytes (P1891A-hiPSC-CMs). Methods We isolated dermal fibroblasts from a symptomatic member of a Finnish family carrying the SCN5A-P1891A variant; derived P1891A-hiPSCs via nucleofection with reprogramming plasmids [4]; and differentiated these into hiPSC-CMs alongside healthy control-hiPSCs. We determined the action potential (AP) and sodium- (INa) and calcium-current (ICa) densities of hiPSC-CMs via patch-clamp. We investigated gene expression in hiPSC-CMs through qPCR and employed Multiple Approaches Combined (MCA) proteomics [5] in SCN5A-transfected HEK293-like cells to elucidate protein-protein interactions (PPI). We subjected hiPSC-CMs to mechanical stretch [6] to examine their stress response and assessed their proliferation capacity via high-content analysis for the percentage of BrdU positive hiPSC-CMs following mitogenic stimulation (5 µM CHIR99021 and 1 µM SB203580). Finally, we ascertained contractile kinetics and apparent contractile force using fibrin hydrogel-based engineered heart tissues (EHTs) [7]. Results The P1891A-hiPSC-CMs demonstrated elevated INa density alongside enhanced arrhythmogenicity relative to healthy control hiPSC-CMs although, AP kinetics and ICa density were unperturbed (Fig. 1). Under baseline conditions, P1891A-hiPSC-CMs exhibited higher SCN5A gene expression and proteomics revealed a reduction in PPI. Baseline proliferation was unchanged; however, aged P1891A-hiPSC-CMs had enhanced proliferative capacity following mitogenic stimulation. Further, the P1891A-hiPSC-CMs displayed impaired tolerance to mechanical stretch as evidenced by upregulated NPPB and ACTA1 expression. In the context of the EHT, P1891A-hiPSC-CMs yielded disparate phenotypes. Whilst a subset compacted fully and yielded weaker contractile parameters relative to healthy control EHTs, the majority of P1891A-hiPSC-CM EHTs failed to fully compact thereby recapitulating the LVNC phenotype (Fig. 2). Conclusions This study presents a unique aetiology of LVNC and links, for the first time, SCN5A mutations with enhanced human cardiomyocyte proliferation. The ability to recapture the noncompaction phenotype in EHTs presents a powerful model for future mechanistic research and drug development.

Development of Ethnosciene Reels of Ketapang Malay Tribe as Supplementary Learning Resources on Hydrocarbon Compounds

The habit of the Malay community in using natural materials as fuel can be integrated into chemistry teaching materials in schools. The aim of this research is to assess the validity and practicality of reels as a learning resource and to develop reels based on the ethnoscience of the Ketapang Malay tribe. The research development model used is the Three Stages Research and Development (TSRD) model, which consists of three steps: planning, development, and dissemination. Data collection techniques involve indirect communication using validation sheets and response instruments. The subjects of the research are students from SMA N 1 Sungai Laur. The results of the study indicate that the ethnoscience-based reels of the Malay tribe are valid and practical, with validity and practicality scores of 96.3% and 90.30%, respectively. The reels can be used as an independent, practical, and engaging learning resource that is relevant to students. Therefore, the validity and practicality results of the development of ethnoscience-based reels are expected to serve as an innovative and contextual alternative media for chemistry education in schools.

Development of Mini Virtual Aquatic Ecosystem Learning Media Assisted by Professional 3D Pageflip Software

This development aims to produce a mini virtual learning media product for aquatic ecosystems assisted by professional 3D pageflip software. The development research model used is the Four-D (4D) model, which consists of 4 stages including: (1) define stage, (2) design stage, (3) develop stage, practicality testing is carried out. , and the dissemination stage was not carried out due to time constraints. The trial subjects in this research involved 36 students of SMAN 9 Mataram class X1, and involved teachers as user practitioners and observers to assess the implementation of learning. The data collection techniques used consisted of learning implementation questionnaire sheets, teacher response questionnaire sheets and student responses. The data analysis used is quantitative and qualitative. Based on the results of the practicality test, it can be concluded that the virtual aquatic ecosystem mini media is very practical to use. The teacher's response showed a percentage of 89.32 in the very practical category, and the student response showed a percentage of 86.04 in the very practical category. Meanwhile, learning implementation is 90%, and non-implementation is 10%.