In this paper, the investigation focused on natural ventilation induced by wind and buoyancy effects through a wind tunnel experiment. A scaled-down model of a single-zone building featuring two opposing openings at varying heights was subjected to uniform heating from a source on the interior floor. Wind pressure coefficient differences were measured in a sealed model to assess the wind’s driving force. Indoor and outdoor temperatures were recorded to ascertain the buoyancy-driven driving force. The ventilation rate was assessed using the tracer gas constant emission method, while airflow characteristics were quantified through particle image velocimetry (PIV). The comparison between predicted and measured ventilation rates revealed generally good accuracy in cases where wind assists buoyancy. However, errors were evident in cases of opposing wind and buoyancy due to neglecting the effect of wind turbulence. The airflow in cases of opposing wind and buoyancy effects demonstrates the most dynamic changes, as illustrated through flow visualizations and transient temperature fluctuations at the openings.