Mode Of Migration Research Articles

Z-scheme 0D/3D p-Ag6Si2O7 nanoparticles-decorated n-Bi2O2CO3 micro-flowers heterojunction photocatalyst for efficient degradation of organic contaminants

Novel Z-scheme 0D/3D p-Ag6Si2O7/n-Bi2O2CO3 micro-flowers heterojunctions were fabricated by a CTAB-aided deposition and in-situ growth route for the first time. The photocatalysts were characterized systematically via XRD, XPS, SEM, BET, EDS, TEM and Mott-Schottky. Under simulated sunlight irradiation, the AB0.15 (the molar ratio of Ag6Si2O7/Bi2O2CO3 is 0.15:1) presented optimal removal rate of methyl orange (0.1064 min−1), which was 18 and 15 times that of Bi2O2CO3 and Ag6Si2O7, respectively. Simultaneously, the AB0.15 can effectively eliminate colourless ciprofloxacin from water (0.0768 min−1), which ruled out the photosensitization. The outstanding organics mineralization ability and photocatalytic stability of AB0.15 were verified by TOC and cyclic tests. As evidenced by UV–vis DRS, PL and electrochemical experiments, AB0.15 exhibited broaden light absorption and improved charge separation efficiency compared with single-phase samples. Trapping experiments and ESR electron spin test proved that h+ and •O2− were principal active species during the degradation. The performance enhancement was mainly ascribed to the synergistic action of p-n heterojunction built-in electric field and Z-scheme charge migration mode, extremely promoted the activization and separation of carriers. This study offers a new perspective for designing high-performance Z-scheme photocatalysts for sewage purification.

Coupling traction force patterns and actomyosin wave dynamics reveals mechanics of cell motion

Motile cells can use and switch between different modes of migration. Here, we use traction force microscopy and fluorescent labeling of actin and myosin to quantify and correlate traction force patterns and cytoskeletal distributions in Dictyostelium discoideum cells that move and switch between keratocyte‐like fan‐shaped, oscillatory, and amoeboid modes. We find that the wave dynamics of the cytoskeletal components critically determine the traction force pattern, cell morphology, and migration mode. Furthermore, we find that fan‐shaped cells can exhibit two different propulsion mechanisms, each with a distinct traction force pattern. Finally, the traction force patterns can be recapitulated using a computational model, which uses the experimentally determined spatiotemporal distributions of actin and myosin forces and a viscous cytoskeletal network. Our results suggest that cell motion can be generated by friction between the flow of this network and the substrate.

Metabolites and metabolic pathways of maackiain in rats based on UPLC-Q-TOF-MS

Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used to investigate the metabolites of maackiain in rats based on the prediction function of UNIFI data processing system and liver microsomal incubation in vitro. Ten metabolites of maackiain after oral absorption were reasonably deduced and characterized. It was found that the biotransformation of maackiain mainly included phase Ⅰ oxidation, dehydrogenation, phase Ⅱ sulfate conjugation, glucosylation conjugation, and glucuronic acid conjugation. Among them, the product of glucosylation conjugation, trifolirhizin, was identified by comparison with the reference for the first time. Liver microsomal incubation in vitro further confirmed the metabolites and metabolic pathways of maackiain in rats. The metabolites in the blood, urine, and feces complemented each other, which revealed the migration, metabolism, and excretion modes of maackiain in rats. This study lays a foundation for the further investigation of the metabolic mechanism of maackiain in vivo and the in-depth research on the mechanism of pharmacodynamics and toxicity.

Acoustic particle migration and focusing in a tilted acoustic field

Surface acoustic wave-based particle/bioparticle manipulation has emerged as a promising tool for disease diagnosis. The effects of the titled angle of the acoustic field θ and the microchannel aspect ratios β on the particle migration mode, the force of particle, and the three-dimensional focusing behavior are studied by using simulation and high-speed microscopic visualizations experiments. The acoustic field tilt range is from 0° to 15°, and the wavelength is 160 μm. Particle migration trajectory is observed from high-speed photographic images. Compared with most parallel acoustic fields, the particle migration efficiency of the tilted acoustic field is higher because the acoustic radiation force (Fr) continues to act on the particles in the lateral direction. The tilted angle of the acoustic field is not a fixed value (usually 15°), and there is an optimal angle to match the maximum lateral migration of the target particles. A model is put forward to predict the optimal acoustic field tilt-angle for acoustofluidic devices, which can achieve 96% separation of 15 μm target particles. The change in the direction of the Fr drives the particles to create two typical migration states during the lateral migration process, named continuous migration and intermittent migration. The phenomenon of multi-layer particle focus in the vertical Z-direction of the microchannel is experimentally observed for the first time, which mainly depends on whether the microchannel has enough height to make multiple acoustic pressure nodes in the vertical direction. Two or even three layers of particle focus lines can be observed in the vertical direction at the microchannel aspect ratios β > 0.5. The research results provide new insight into the high-throughput development of microfluidic devices.

Effect of walls on the motion of magnetically driven superparamagnetic microparticles

In microfluidics studies, it is often (implicitly) assumed that particles follow the surrounding flow. The effect of boundaries is, therefore, neglected. In the present paper, we investigate—experimentally and theoretically—the situation wherein the presence of boundaries affects the dynamics of particles. We designed and fabricated a tool to detect particle positions and measure their displacement and velocity to study the effect of wall on magnetically driven superparamagnetic microparticles (SMP). Two experimental setups are employed. A vertical setup is operated in free particle migration mode where the particle motion is not affected by the chip boundaries. We here measure the density and the magnetic susceptibility of the particle. This setup allows to characterize particle properties and to calibrate the interaction of the particles with the external magnetic field. In a horizontal setup, we track migration with the particle motion affected by the presence of channel bottom.

Channel Migration of the Meandering River Fan: A Case Study of the Okavango Delta

Okavango delta is a typical distributive fluvial system, which is composed of a series of sand island-river-swamp networks. River migration in the Okavango delta is analyzed by using satellite images from Google Earth and Alaska Satellite Facility (ASF). Four configuration characterization parameters are selected to depict and measure the meandering river. These four parameters are sinuosity index (S), curvature (C), the difference of along-current deflection angle (Δθ) and expansion coefficient (Km). In the fan, the channel migration is mainly asymmetric. According to geomorphic elements and associated features, Okavango Delta can be subdivided into three zones: axial zone, median zone and distal zone. Under the influence of slope, climate and vegetation, different migration modes are developed in different zones. As the river moves downstream, the sinuosity index of the river on the Okavango Delta decreases downstream. Based on the characteristics of different zones, the sedimentary facies model of a single source distributive fluvial system of a meandering river is proposed. The models of channel migration and sedimentary facies have wide application. This research will not only provide a basis for the prediction of future river channels but will also provide important theoretical guidance for the study of the sedimentary morphology of underground reservoirs.

Breakthrough pressure anisotropy and intra-source migration model of crude oil in shale

With shale oil becoming an increasingly important resource in global oil and gas exploration and development, the breakthrough pressure of crude oil in shale series source rocks has become an important topic for research on hydrocarbon migration. Shale has long been recognized as an effective source and cap rock, with, up to now, the most common method used for breakthrough pressure testing in shale being the gas method. However, this paper sets out a new system for testing the breakthrough pressure of crude oil in siltstone, mudstone, and shale based on differences in electrical resistance between crude oil and formation water. The breakthrough pressure of crude oil with different lithology was tested in different directions using this method. The results show that breakthrough pressure anisotropy can be observed in mudstone, shale, and siltstone, and that the breakthrough pressure parallel to the bedding direction is one fourth or one seventh of the breakthrough pressure perpendicular to the bedding direction. The horizontal breakthrough pressure of shale is one twentieth to one third of that of mudstone and siltstone, and the vertical breakthrough pressure of siltstone is one seventh of that of mudstone and shale. The breakthrough pressure anisotropy of crude oil reveals that the main migration direction in shale series source rocks is in the direction of bedding unless faults or fractures are encountered. The main migration channels in shale series source rocks are in the horizontal direction, and hydrocarbon migration occurs more easily in laminar shale than in mudstone. Horizontal migration is also the principal mode of hydrocarbon expulsion in thick mudstone and shale. The breakthrough pressure experiments on crude oil in this study also reveal the migration directions and modes in intra-source rocks. This improves on the existing oil migration model and has great significance for understanding the migration of oil in shale source rocks.

Time‐Scales of a Dune‐Beach System and Implications for Shoreline Modeling

AbstractUnderstanding the interactions between dune systems and beaches is critical to determining the short‐term shoreline response and the long‐term resilience. In this study, almost 15 years of monthly beach/dune measurements were analyzed for three different profiles at Vougot Beach, France to understand and predict shoreline changes from intra‐annual to multi‐annual time‐scales. Four migration modes: advance/retreat (translation modes) and steepening/flattening (rotation modes) were identified through a centroid analysis. The analysis showed that translation and rotation can occur simultaneously, with long‐term trends of beach retreat and profile steepening (lower beach retreating and upper beach advancing), which was interrupted by two energetic wave events causing profile flattening (lower beach advancing and upper beach retreating). These two observations are evidence of how the sediment contribution resulting from the dune erosion events temporarily caused a large advance in the shoreline position. A recent modeling approach that accounts for different time‐scales is applied to predict the shoreline changes, showing significant improvements in comparison to a traditional shoreline equilibrium model when time‐scales related with the dune erosion and recovery are considered. The results showed that the dune system affects the beach profile evolution both spatially, with different impacts at different elevations along the cross‐shore profile, and temporally, by periodically redistributing the sediment in the system.

Relationship between Blood Vessels and Migration of Neuroblasts in the Olfactory Neurogenic Region of the Rodent Brain.

Neural precursors originating in the subventricular zone (SVZ), the largest neurogenic region of the adult brain, migrate several millimeters along a restricted migratory pathway, the rostral migratory stream (RMS), toward the olfactory bulb (OB), where they differentiate into interneurons and integrate into the local neuronal circuits. Migration of SVZ-derived neuroblasts in the adult brain differs in many aspects from that in the embryonic period. Unlike in that period, postnatally-generated neuroblasts in the SVZ are able to divide during migration along the RMS, as well as they migrate independently of radial glia. The homophilic mode of migration, i.e., using each other to move, is typical for neuroblast movement in the RMS. In addition, it has recently been demonstrated that specifically-arranged blood vessels navigate SVZ-derived neuroblasts to the OB and provide signals which promote migration. Here we review the development of vasculature in the presumptive neurogenic region of the rodent brain during the embryonic period as well as the development of the vascular scaffold guiding neuroblast migration in the postnatal period, and the significance of blood vessel reorganization during the early postnatal period for proper migration of RMS neuroblasts in adulthood.

Bidirectional Mechanical Response Between Cells and Their Microenvironment

Cell migration and invasion play a role in many physiological and pathological processes and are therefore subject of intensive research efforts. Despite of the intensively investigated biochemical processes associated with the migration and invasion of cells, such as cancer cells, the contribution of mechanobiological processes to the migratory capacity of cells as well as the role of physical polymeric phase transitions is not yet clearly understood. Unfortunately, these experiments are not very informative because they completely disregard the influence of the three-dimensional cell environment. Despite this data situation, it was possible to adequately demonstrate that there exists a direct mechanical interplay between cells and their microenvironment in both directions, where both elements can be mechanically altered by one another. In line with these results, it has turned out that the mechanobiological molecular processes through which cells interact with each other and additionally sense their nearby microenvironment have an impact on cellular functions such as cellular motility. The mechanotransduction processes have become the major focus of biophysical research and thereby, diverse biophysical approaches have been developed and improved to analyze the mechanical properties of individual cells and extracellular matrix environments. Both, the cell mechanics and matrix environment mechanics regulate the cell migration types in confined microenvironments and hence it seems to be suitable to identify and subsequently present a common bidirectional interplay between cells and their matrix environment. Moreover, hallmarks of the mechanophenotype of invasive cells and extracellular matrices can be defined. This review will point out how on the one hand the intracellular cytoskeletal architecture and on the other hand the matrix architecture contribute to cellular stiffness or contractility and thereby determines the migratory phenotype and subsequently the emergence of a distinct migration mode. Finally, in this review it is discussed whether universal hallmarks of the migratory phenotype can be defined.

Low-cost polymer-film spiral inertial microfluidic device for label-free separation of malignant tumor cells.

We developed a low-cost polymer-film spiral inertial microfluidic device for the effective size-dependent separation of malignant tumor cells. The device was fabricated in polymer films by rapid laser cutting and chemical bonding. After fabricating the prototype device, the separation performance of our device was evaluated using particles and cells. The effects of operational flow rate, cell diameter, and cell concentration on the separation performance were explored. Our device successfully separated tumor cells from polydisperse white blood cells according to their different migration modes and lateral positions. Then, the separation of rare cells was carried out using the high-concentration lysed blood spiked with 200 tumor cells. Experimental results showed that 83.90% of the tumor cells could be recovered, while 99.87% of white blood cells could be removed. We successfully employed our device for processing clinical pleural effusion samples from patients with advanced metastatic breast cancer. Malignant tumor cells with an average purity of 2.37% could be effectively enriched, improving downstream diagnostic accuracy. Our device offers the advantages of label-free operation, low cost, and fast fabrication, thus being a potential tool for effective cell separation.

Morphodynamics facilitate cancer cells to navigate 3D extracellular matrix

Cell shape is linked to cell function. The significance of cell morphodynamics, namely the temporal fluctuation of cell shape, is much less understood. Here we study the morphodynamics of MDA-MB-231 cells in type I collagen extracellular matrix (ECM). We systematically vary ECM physical properties by tuning collagen concentrations, alignment, and gelation temperatures. We find that morphodynamics of 3D migrating cells are externally controlled by ECM mechanics and internally modulated by Rho/ROCK-signaling. We employ machine learning to classify cell shape into four different morphological phenotypes, each corresponding to a distinct migration mode. As a result, we map cell morphodynamics at mesoscale into the temporal evolution of morphological phenotypes. We characterize the mesoscale dynamics including occurrence probability, dwell time and transition matrix at varying ECM conditions, which demonstrate the complex phenotype landscape and optimal pathways for phenotype transitions. In light of the mesoscale dynamics, we show that 3D cancer cell motility is a hidden Markov process whereby the step size distributions of cell migration are coupled with simultaneous cell morphodynamics. Morphological phenotype transitions also facilitate cancer cells to navigate non-uniform ECM such as traversing the interface between matrices of two distinct microstructures. In conclusion, we demonstrate that 3D migrating cancer cells exhibit rich morphodynamics that is controlled by ECM mechanics, Rho/ROCK-signaling, and regulate cell motility. Our results pave the way to the functional understanding and mechanical programming of cell morphodynamics as a route to predict and control 3D cell motility.

Melanoma cells adopt features of both mesenchymal and amoeboid migration within confining channels

For metastasis to occur, cancer cells must traverse a range of tissue environments. In part, this is accomplished by cells adjusting their migration mode to one that is best suited to the environment. Melanoma cells have been shown to be particularly plastic, frequently using both mesenchymal and amoeboid (bleb-based) modes of migration. It has been demonstrated that 2D confinement will promote the transition from mesenchymal to bleb-based migration. However, if melanoma cells similarly transition to bleb-based migration in response to 3D confinement, such as within narrow channels, is unknown. Here, using micro-fabricated channels, we demonstrate that metastatic, A375-M2, melanoma cells adopt features of both mesenchymal and bleb-based migration. In narrow (8 µm; height and width) channels coated with fibronectin, ~ 50% of melanoma cells were found to use either mesenchymal or bleb-based migration modes. In contrast, the inhibition of Src family kinases or coating channels with BSA, completely eliminated any features of mesenchymal migration. Detailed comparisons of migration parameters revealed that blebbing cells, particularly in the absence of adhesions, were faster than mesenchymal cells. In contrast to what has been previously shown under conditions of 2D confinement, pharmacologically inhibiting Arp2/3 promoted a fast filopodial-based mode of migration. Accordingly, we report that melanoma cells adopt a unique range of phenotypes under conditions of 3D confinement.

Analysis of Dynamic Changes of Lipid Composition and Structure of Deep‐Fried Pork Slices during Storage

AbstractTo understand the reasons for the quality deterioration of traditional battered and deep‐fried pork slices during storage, fried pork slices stored for 12 h at 0–40 °C are used as the research samples, and Fourier‐transform infrared spectroscopy （FTIR） and gas chromatography‐mass spectrometry （GC‐MS） are used as analytical methods. The oil migration mode, fatty acid composition change, and functional group changes in the meat slices are analyzed. The experimental results show that the unsaturated fatty acid (USFA) in the starch paste enters the meat slice in a single diffusion mode during storage, and the migration direction of the fatty acid is reversed after 7 h. Oxidation mainly occurs after 4 h of storage. At this time, the absorption peak intensities of the C═C, OH, CH2, and CH3 groups began to increase significantly. After 8 h, the characteristic peak of C═O narrowed and disappeared after 12 h, indicating that the oxidation has entered a decline period. The content of USFA and linoleic acid (C18:2) showed a downward trend in phospholipids. Therefore, the stability of phospholipids, especially linoleic acid, plays an important role in the edibility of meat slices during storage.Practical Applications: Many studies have demonstrated that the edible quality of fried meat products decreases gradually during storage and the change in lipid content is the main reason for this phenomenon. However, there is a lack of targeted studies on lipid migration and compositional and structural changes during the storage of deep‐fried foods. In this study, dynamic mobility, dynamic modeling, and changes in fatty acid composition and structure of lipids before and after storage provide data support for the implementation of targeted improvement measures to reduce oil migration, oxidation, and decomposition, and thus effectively extend the consumption cycle.

Three-dimensional migration of human amniotic fluid stem cells involves mesenchymal and amoeboid modes and is regulated by mTORC1.

Three‐dimensional (3D) cell migration is an integral part of many physiologic processes. Although being well studied in the context of adult tissue homeostasis and cancer development, remarkably little is known about the invasive behavior of human stem cells. Using two different kinds of invasion assays, this study aimed at investigating and characterizing the 3D migratory capacity of human amniotic fluid stem cells (hAFSCs), a well‐established fetal stem cell type. Eight hAFSC lines were found to harbor pronounced potential to penetrate basement membrane (BM)‐like matrices. Morphological examination and inhibitor approaches revealed that 3D migration of hAFSCs involves both the matrix metalloprotease‐dependent mesenchymal, elongated mode and the Rho‐associated protein kinase‐dependent amoeboid, round mode. Moreover, hAFSCs could be shown to harbor transendothelial migration capacity and to exhibit a motility‐associated marker expression pattern. Finally, the potential to cross extracellular matrix was found to be induced by mTORC1‐activating growth factors and reduced by blocking mTORC1 activity. Taken together, this report provides the first demonstration that human stem cells exhibit mTORC1‐dependent invasive capacity and can concurrently make use of mesenchymal and amoeboid 3D cell migration modes, which represents an important step toward the full biological characterization of fetal human stem cells with relevance to both developmental research and stem cell‐based therapy.

The Research on the Product Humanization Model Based on the Evolution of Consumer Purchase Pat

Digital economy gives new connotation to business model. In the process of business model changing from vertical to horizontal, from exclusive to inclusive, and from individual to social, the central activity of enterprise marketing should be transferred to how to actively interact with consumers, so that consumers can more participate in the process of enterprise marketing value creation. This paper focuses on the model of consumers’ participation in marketing value creation. Firstly, this paper studies the basic model of consumer purchase path evolution, and proposes 5A +1D model based on the basic model. Then, based on the 5A+1D model, an important carrier for consumers to participate in the value creation of marketing is proposed: the product humanization model. Secondly, the paper analyzes the ideal migration mode of consumer purchase path under humanistic products: “W” model, which finally reaches the balance at both ends of 5A+1D model. Under the ecological background of open innovation, this paper extends the chain of consumers’ purchase path and realizes the co-creation of consumers and enterprises’ value, and designs a human-oriented product model based on the chain of consumers’ extended purchase path. The W model achieves the balance between the beginning and the end of the consumer purchase path model, which provides a theoretical basis for the intervention points of enterprises on the overall consumers’ purchase path.

Cell scientist to watch – Verena Ruprecht

ABSTRACT Verena Ruprecht received her PhD in Biophysics from the Johannes Kepler University in 2010 for her work on developing single-molecule super-resolution imaging tools in the lab of Gerhard Schütz. Following a research visit in Didier Marguet's lab at the Centre d'Immunologie de Marseille-Luminy (CIML) in France, she moved to the Institute of Science and Technology (IST) in Austria for a postdoc, working jointly with Carl-Philipp Heisenberg and Michael Sixt. There, she discovered a unique amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Verena started her independent laboratory at the Centre for Genomic Regulation (CRG) in Barcelona, Spain, in September 2016. Her group combines genetic and biophysical methods with multi-scale imaging and mathematical modelling to study cellular dynamics in embryo development. In 2020, Verena was selected as an EMBO Young Investigator and in the same year awarded an HFSP Young Investigator Grant for a collaborative project to study the biophysics of zebrafish fertilization.

Live imaging of remyelination in the adult mouse corpus callosum

Oligodendrocyte precursor cells (OPCs) retain the capacity to remyelinate axons in the corpus callosum (CC) upon demyelination. However, the dynamics of OPC activation, mode of cell division, migration, and differentiation on a single-cell level remain poorly understood due to the lack of longitudinal observations of individual cells within the injured brain. After inducing focal demyelination with lysophosphatidylcholin in the CC of adult mice, we used two-photon microscopy to follow for up to 2 mo OPCs and their differentiating progeny, genetically labeled through conditional recombination driven by the regulatory elements of the gene Achaete-scute homolog 1. OPCs underwent several rounds of symmetric and asymmetric cell divisions, producing a subset of daughter cells that differentiates into myelinating oligodendrocytes. While OPCs continue to proliferate, differentiation into myelinating oligodendrocytes declines with time, and death of OPC-derived daughter cells increases. Thus, chronic in vivo imaging delineates the cellular principles leading to remyelination in the adult brain, providing a framework for the development of strategies to enhance endogenous brain repair in acute and chronic demyelinating disease.

Nonlinear dynamics of cell migration in anisotropic microenvironment* *Project supported by the National Natural Science Foundation of China (Grant Nos. 11974066, 11674043, 11675134, and 11874310), the Natural Science Foundation of Chongqing, China (Grant Nos. cstc2019jcyj-msxmX0477 and cstc2018jcyjA3679), and the Capital Health Development Research Project, China (Grant No. 2020-2-2072).

Cell migration in anisotropic microenvironment plays an important role in the development of normal tissues and organs as well as neoplasm progression, e.g., osteogenic differentiation of embryonic stem cells was facilitated on stiffer substrates, indicating that the mechanical signals greatly affect both early and terminal differentiation of embryonic stem cells. However, the effect of anisotropy on cell migration dynamics, in particular, in terms of acceleration profiles which is important for recognizing dynamics modes of cell migration and analyzing the regulation mechanisms of microenvironment in mechanical signal transmission, has not been systematically investigated. In this work, we firstly rigorously investigate and quantify the differences between persistent random walk and anisotropic persistent random walk models based on the analysis of cell migration trajectories and velocity auto-covariance function, both qualitatively and quantitatively. Secondly, we introduce the concepts of positive and negative anisotropy based on the motility parameters to study the effect of anisotropy on acceleration profiles, especially the nonlinear decrease and non-monotonic behaviors. We particularly elaborate and discuss the mechanisms, and physical insights of non-monotonic behaviors in the case of positive anisotropy, focusing on the force exerted on migrating cells. Finally, we analyze two types of in vitro cell migration experiments and verify the universality of nonlinear decrease and the consistence of non-monotonic behaviors with numerical results. We conclude that the anisotropy of microenvironment is the cause of the non-monotonic and nonlinear dynamics, and the anisotropic persistent random walk can be as a suitable tool to analyze in vitro cell migration with different combinations of motility parameters. Our analysis provides new insights into the dynamics of cell migration in complex microenvironment, which also has implications in tissue engineering and cancer research.

Influence Factors of Pressure Attenuation Gradient and its Reservoir Forming Control - A Case Study of Bonan Sag in Bohai Bay Basin

As the dynamic condition of oil and gas migration, the change rate of overpressure has a certain relationship with the mode of oil and gas migration. In order to quantitatively identify the mode of oil and gas migration in overpressure basins, the influence of different transmission modes on pressure attenuation gradient is simulated, and the application and discussion are carried out in Bonan sag of Bohai Bay Basin. The influence of different transmission modes on the pressure attenuation gradient is discussed by single factor simulation method. Taking Bonan sag as an example, the process of overpressure fluid migration and accumulation is discussed combined with the evolution process of pressure attenuation gradient. The results show that fault activity has the greatest influence on pressure attenuation gradient, followed by fault dip angle and fault distance. the influence degree of physical properties of skeleton sand body, sand body dip angle and sand body thickness on pressure attenuation gradient decreases in turn; For the fault-sand consistent transport, it mainly depends on the combined effect of the dip angle and resistance of the transport system. The critical value of the fault or sand body transport in Bonan sag is 58.8MPa/km. On this basis, combined with the evolution law of pressure attenuation gradient, it is considered that the overpressure fluid in the deep sag migrates along the oil source fault to the fault terrace zone and the overpressure center distribution of the oil and gas ring in the deep sag. The overpressure oil and gas mainly migrate laterally along the sand body, and the oil and gas are distributed contiguously. In gentle slope zone, oil and gas distribute sporadically at structural height.