Mode Of Entry Research Articles

Female-specific mechanisms of meiotic initiation and progression in mammalian oocyte development.

Meiosis is regulated in sexually dimorphic manners in mammals. In females, the commitment to and entry into meiosis are coordinated with the developmental program of oocytes. Female germ cells initiate meiosis within a short time window during the fetal period and then undergo meiotic arrest until puberty. However, the genetic mechanisms underlying the orchestration of oocyte development and meiosis to maximize the reproductive lifespan of mammalian females remain largely elusive. While meiotic initiation is regulated by a sexually common mechanism, where meiosis initiator and Stimulated by Retinoic Acid Gene 8 (STRA8) activate the meiotic genes, the female-specific mode of meiotic initiation is mediated by the interaction between retinoblastoma (RB) and STRA8. This review highlights the female-specific mechanisms of meiotic initiation and meiotic prophase progression in the context of oocyte development. Furthermore, the downstream pathway of the RB-STRA8 interaction that may regulate meiotic arrest will be discussed in the context of oocyte development, highlighting a potential genetic link between the female-specific mode of meiotic entry and meiotic arrest.

Uncovering the coronavirus outbreak: present understanding and future research paths.

The review discusses the pathophysiological mechanisms of SARS-CoV-2, the modes of transmission, and the long-term health consequences of COVID-19, emphasizing the importance of research and successful public health initiatives. COVID-19 taxonomy, pathophysiology, symptomatology, and epidemiological importance are the key objects of this research paper. This review explains how COVID-19 affects different systems of the body, including respiratory, cardiovascular, and reproductive systems of the human body. It describes the modes of entry of the virus into the cell; more precisely, ACE2 and TMPRSS2 in viral entry. In addition, the present study analyzes the situation of COVID-19 in India regarding vaccine development and the transmission rate related to socioeconomic factors. The manifestation of COVID-19 presents a lot of symptoms and post-acute problems, issues which are seriously impacting mental health and physical health as well. The present review summarizes current research into pathogenicity and the mode of virus transmission, together with immunological responses. Coupled with strong vaccination programs, public health initiatives should hold the key to fighting this pandemic. Long-term effects and the development of treatment methods will need further study, as ambiguities on COVID-19 remain. Multidisciplinary collaboration across healthcare sectors in this respect is of paramount importance for the prevention of further spread and protection of public health.

Tailoring the magnetocaloric effect in doped GaAs quantum dot under the influence of noise

Present inspection thoroughly explores the magnetocaloric effect (MCE) of GaAs quantum dot (QD) doped with Gaussian impurity and under the influence of Gaussian white noise (GWHN). Two different routes for the application of GWHN viz. additive and multiplicative have been considered. The enquiry highlights extremely subtle and complex interplay between temperature, GWHN, mode of entry of GWHN and the given physical parameters concerned that finally designs the MCE profiles. The fact that under certain conditions the MCE maximizes comes out to be technologically relevant. Deviations in the MCE profiles from the noise-free state appear to be more profound under multiplicative noise than its additive analogue. As a general trend, additive and multiplicative noise reduces and augments the MCE, respectively, from that under the noise-free situation.

Interrogating the Role of Endocytosis Pathway and Organelle Trafficking for Doxorubicin-Based Combination Ionic Nanomedicines.

We have studied the endocytic mechanisms that determine subcellular localization for three carrier-free chemotherapeutic-photothermal (chemo-PTT) combination ionic nanomedicines (INMs) composed of doxorubicin (DOX) and an near-infrared (NIR) dye (ICG, IR820, or IR783). This study aims to understand the cellular basis for previously published enhanced toxicity results of these combination nanomedicines toward MCF-7 breast cancer cells. The active transport mechanism of INMs, unlike free DOX, which is known to employ passive transport, was validated by conducting temperature-dependent cellular uptake of the drug in MCF-7 cells using confocal microscopy. The internalization pathway of these INMs was further probed in the presence and absence of different endocytosis inhibitors. Detailed examination of the mode of entry of the carrier-free INMs in MCF-7 cells revealed that they are primarily internalized through clathrin-mediated endocytosis. In addition, time-dependent subcellular localization studies were also investigated. Examination of time-dependent confocal images indicated that the INMs targeted multiple organelles, in contrast to free DOX that primarily targets the nucleus. Collectively, the high cellular endocytic uptake in cancerous cells (EPR effect) and the multimode targeting ability demonstrated the main reason for the low half-maxima inhibitory concentration (IC50) value (the high cytotoxicity) of these carrier-free INMs as compared to their respective parent chemo and PTT drugs.

The role of autophagy in the progression of HIV infected cardiomyopathy.

Although highly active antiretroviral therapy (HAART) has changed infection with human immunodeficiency virus (HIV) from a diagnosis with imminent mortality to a chronic illness, HIV positive patients who do not develop acquired immunodeficiency syndrome (AIDs) still suffer from a high rate of cardiac dysfunction and fibrosis. Regardless of viral load and CD count, HIV-associated cardiomyopathy (HIVAC) still causes a high rate of mortality and morbidity amongst HIV patients. While this is a well characterized clinical phenomena, the molecular mechanism of HIVAC is not well understood. In this review, we consolidate, analyze, and discuss current research on the intersection between autophagy and HIVAC. Multiple studies have linked dysregulation in various regulators and functional components of autophagy to HIV infection regardless of mode of viral entry, i.e., coronary, cardiac chamber, or pericardial space. HIV proteins, including negative regulatory factor (Nef), glycoprotein 120 (gp120), and transactivator (Tat), have been shown to interact with type II microtubule-associated protein-1 β light chain (LC3-II), Rubiquitin, SQSTM1/p62, Rab7, autophagy-specific gene 7 (ATG7), and lysosomal-associated membrane protein 1 (LAMP1), all molecules critical to normal autophagy. HIV infection can also induce dysregulation of mitochondrial bioenergetics by altering production and equilibrium of adenosine triphosphate (ATP), mitochondrial reactive oxygen species (ROS), and calcium. These changes alter mitochondrial mass and morphology, which normally trigger autophagy to clear away dysfunctional organelles. However, with HIV infection also triggering autophagy dysfunction, these abnormal mitochondria accumulate and contribute to myocardial dysfunction. Likewise, use of HAART, azidothymidine and Abacavir, have been shown to induce cardiac dysfunction and fibrosis by inducing abnormal autophagy during antiretroviral therapy. Conversely, studies have shown that increasing autophagy can reduce the accumulation of dysfunctional mitochondria and restore cardiomyocyte function. Interestingly, Rapamycin, a mammalian target of rapamycin (mTOR) inhibitor, has also been shown to reduce HIV-induced cytotoxicity by regulating autophagy-related proteins, making it a non-antiviral agent with the potential to treat HIVAC. In this review, we synthesize these findings to provide a better understanding of the role autophagy plays in HIVAC and discuss the potential pharmacologic targets unveiled by this research.

Chinese Multinationals Gaining Global Dominance

Chinese firms have become key players in the global economy. Their rise is a defining feature since the turn of the century. In this paper, we analyze the ascent of Chinese firms in global business, the factors behind their emergence and how they have disrupted the global competitive landscape. Through the empirical study of large samples of firms and the help of databases like Capital IQ, FdI Markets and SDC Platinum, the paper examines the revenue growth and global expansion of Chinese firms– highlighting, among others, trends in geographical and sectoral distribution and modes of entry in foreign markets. The paper pays also particular attention to the rise of Chinese firms as global innovators and includes several cases studies in key industries. The paper concludes that while Chinese firms’ global expansion benefited from a favorable global environment, the current trade wars and geopolitical tensions, may be more challenging.

Modulating lipid bilayer permeability and structure: Impact of hydrophobic chain length, C-3 hydroxyl group, and double bond in sphingosine

Sphingosine, an amphiphilic molecule, plays a pivotal role as the core structure of sphingolipids, essential constituents of cell membranes. Its unique capability to enhance the permeability of lipid membranes profoundly influences crucial life processes. The molecular structure of sphingosine dictates its mode of entry into lipid bilayers and governs its interactions with lipids, thereby determining membrane permeability. However, the incomplete elucidation of the relationship between the molecular structure of sphingosine and the permeability of lipid membranes persists due to challenges associated with synthesizing sphingosine molecules. A series of sphingosine-derived molecules, featuring diverse hydrophobic chain lengths and distinct headgroup structure, were meticulously designed and successfully synthesized. These molecules were employed to investigate the permeability of large unilamellar vesicles, functioning as model lipid bilayers. With a decrease in the hydrophobic chain length of sphingosine from C15 to C11, the transient leakage ratio of vesicle contents escalated from ∼ 13 % to ∼ 28 %. Although the presence of double bond did not exert a pronounced influence on transient leakage, it significantly affected the continuous leakage ratio. Conversely, modifying the chirality of the C-3 hydroxyl group gives the opposite result. Notably, methylation at the C-3 hydroxyl significantly elevates transient leakage while suppressing the continuous leakage ratio. Additionally, sphingosines that significantly affect vesicle permeability tend to have a more pronounced impact on cell viability. Throughout this leakage process, the charge state of sphingosine-derived molecule aggregates in the solution emerged as a pivotal factor influencing vesicle permeability. Fluorescence lifetime experiments further revealed discernible variations in the effect of sphingosine molecular structure on the mobility of hydrophobic regions within lipid bilayers. These observed distinctions emphasize the impact of molecular structure on intermolecular interactions, extending to the microscopic architecture of membranes, and underscore the significance of subtle alterations in molecular structure and their associated aggregation behaviors in governing membrane permeability.

Bedside Nurse Documentation Practices: At the Patient Bedside or Not?

High-quality care requires precise and timely provider documentation. Hospitals have used technology to document patient care within both the inpatient and outpatient areas and long-term care facilities. Research has demonstrated, by revealing a reduction in medical errors, that there has been a worldwide improvement in our community health and welfare since the implementation and utilization of documenting patient care electronically. Although electronic documentation has proven to be an improvement in patient record keeping, the most efficient location in which this documentation is to occur remains a question. At the location where this project took place, only the ICU had computers within the patient rooms for documentation purposes. This project evaluated bedside nurses' opinions related to the efficiency of documentation practices compounded by the location where documentation took place. The options were at the patient's bedside, on a workstation on wheels, or at the nursing station. Surveys were provided to bedside nursing staff both before and after computers were installed in patients' rooms in surgical and medical/surgical nursing units at a Veteran Affairs Medical Center located in the Northeastern region of the United States. The results of this project inconclusively answer the question posed: "Which mode of entry do nurses feel is more efficient to document patient care, on a computer in the patient room, at the nurses' station, or on a workstation on wheels?" Innovative strategies should be explored to develop a user-friendly design for computers located within the patient rooms for patient documentation.

Patients suffering traumatic brain injury: patient characteristics, prehospital triage, primary referral and mortality - A population-based follow-up study

BackgroundTraumatic brain injury (TBI) is a potential high-risk condition, but appropriate care pathways, including prehospital triage and primary referral to a specialised neurosurgical centre, can improve neurological outcome and survival. The care pathway starts with layman triage, wherein the patient or bystander decides whether to contact a general practitioner (GP) or emergency services (1-1-2 call) as an entryway into the health care system. The GP or 112-health care professional then decides on the level of urgency and dispatches emergency medical services (EMS) when needed. Finally, a decision is made regarding referral of the TBI patient to a specialised neurotrauma centre or a local hospital. Recent studies have shown that injuries are generally more severe in patients entering the health care system through EMS (112-calls) than through GPs; however, no information exists on whether mortality and morbidity outcomes differ depending on the referral choice. The aim of this study was to examine triage pathways, including the method of entry into the health care system, as well as patient characteristics and place of primary referral, to determine the associated 30-day and 1-year mortality rates in TBI patients with confirmed intracranial lesions.MethodsThis retrospective observational population-based follow-up study was conducted in the Central Denmark Region from 1 February 2017 to 31 January 2019. We included all adult patients who contacted hospitals and were ascribed a predefined TBI ICD-10 diagnosis code in the Danish National Patient Register. The obtained TBI cohort was merged with prehospital data from the Prehospital Emergency Medical Services, Central Denmark Region, and vital status from the Danish Civil Registration System. Binary logistic regression analysis of mortality was conducted. In all patients with TBI (including concussions), the primary outcome was primary referral to a specialised centre based on mode of entry (‘GP/HCP’, ‘112-call’ or ‘Unreferred’) into the health care system. In the subgroup of patients with confirmed intracranial lesions, the secondary outcomes were the relative risk of death at day 30 and 1 year based on the place of primary referral.ResultsOf 5,257 first TBI hospital contacts of adult patients included in the cohort, 1,430 (27.2%) entered the health care system via 1-1-2 emergency medical calls. TBI patients triaged by 112-calls were more likely to receive the highest level of emergency response (15.6% vs. 50.3%; p < 0.001) and second-tier resources and were more frequently referred directly to a specialised centre than were patients entering through GPs or other health care personnel. In the subgroup of 1188/5257 (22.4%) patients with confirmed intracranial lesions, we found no difference in the risk ratio of 30 day (RR 1.04 (95%CI 0.65–1.63)) or 1 year (RR 0.96 (95%CI 0.72–1.25)) all-cause mortality between patients primarily referred to a regional hospital or to a specialised centre when adjusting for age, sex, comorbidities, antiplatelet/anticoagulant treatment and type of intracranial lesions.ConclusionTBI patients mainly enter the health system by contact with GPs or other health care professionals. However, patients entering through 112-calls are more frequently triaged directly to specialised centres. We were unable to demonstrate any significant difference in the adjusted 30-day and 1-year mortality based on e primary referral to a specialised centre. The inability to demonstrate an effect on mortality based on primary referral to a specialised centre may reflect a lack of clinical data in the registries used. Considerable differences may exist in nondocumented baseline characteristics (i.e., GCS, blood pressure and injury severity) between the groups and may limit conclusions about differences in mortality. Further research providing high-quality evidence on the effect of primary referral is needed to secure early neurosurgical interventions in TBI patients.

Footloose and fancy-free in FinTech? Internationalization strategies of born regional and born global firms in the European financial service sector

AbstractDue to economic globalization and technological advancements, businesses are increasingly required to navigate complex international markets. One type of firm that has emerged in response to these trends is the born global, which initiates foreign activities almost from the very start. While these startups expand their reach by pursuing pro-active internationalization strategies, it is unclear whether startups creating the FinTech revolution also belong to this category. This study investigates five key components of the internationalization strategies of European FinTech startups: the speed and scope of internationalization, the drivers of internationalization, international market selection, the mode of market entry, and the challenges involved in internationalization. While born globals use a systematic approach to international market selection and entry, European FinTechs use a quasi-systematic and opportunistic approach to searching and seizing their geographical markets. Their common approach includes quickly going beyond their home base to adjacent markets, followed by gradual experimentation and substantive growth in their home region, and becoming born-regional firms (with some evolving into born-global firms later).

Application Of Social Media Technologies for Research by Final Year University Business Education Students in Universities in Nigeria

This study investigated the application of social media technologies for research by final year university business education students in universities in Nigeria. Four research questions and eight hypotheses guided the study. The study adopted descriptive survey design. The population which served as sample comprised 4,059 final year business education students. A Questionnaire was instrument used for data collection. The questionnaire contained two parts: Part A had 4 items of demographic variables of respondents – name of university, sex, university type (Federal or State), mode of entry. Part B contained 50 items based on the Four Research Questions: Research Question 1 contained 10 items, Research Question 2 contained 11 items, Research Question 3 contained 12 items and Research Question 4 contained 17 items. The questionnaire was structured on a 4-point scale of responses: Very High Extent 4-points, High Extent 3-points, Low Extent 2-points, Very Low Extent 1-point. There was face and content validity of the instrument. The instrument was validated by experts in Computer Science and Measurement and Evaluation at the Delta State University, Abraka. The instrument was subjected to reliability through Cronbach Apha and the result of the four Research Questions are – r=0.82, r=0.87, r=0.89 and r=0.80. A total of 4,059 copies of the questionnaire were administered to 4,059 university final year Business Education students in all universities offering Business Education programmes. 3,896 copies were fully completed and returned indicating a return rate of 95.98%. The data collected were analyzed using mean and standard deviations. The findings were on four major social media websites: web 2.0, WhatsApp, YouTube and Facebook. The study revealed that there is a low extent of use of social media sites for students’ research. It was only Facebook that was established as being used to a high extent for students’ research. It was therefore recommended that University authorities should train students on adequate use of social media for research, social media should be included in the curriculum of universities, Government should provide internet facilities in schools and students should be encouraged to use the school internet facilities for research rather than their own smart phones that could easily lead to distraction.

Survival Analysis of Students’ Dropout in a Nigerian University System

Formal education remains the vehicle for socio-economic development and social mobilization in any society. Academic institutions are increasingly interested in monitoring the performance of their students. This interest has given rise to the need for research, collation, analysis, and interpretation of data to formulate evidence-based academic policies that aim to improve student performance and reduce dropout rates. Data obtained from the university comprises of Gender, Age at Entry, Marital Status, Sponsorship, Mode of Entry, Program of Study and Cumulative Grade Point Average (CGPA). This study investigated how these factors influence students’ dropout at Modibbo Adama University, Yola Adamawa State Nigeria. Survival analysis was carried out using Log-logistic, Lognormal, Weibull and Cox Proportional Hazard models. The lognormal survival model was judged as the best model due to its lowest AIC value of 1199.773. The Gender, CGPA and students’ program of study have significant effect on student dropout. Also, it was observed that 41.42% of male students dropped out when compared to 38.94% female students dropped out before their graduation year. Of all the programs of study considered, Physics has the highest number of dropped out before graduation. It accounted for 68.75%. In terms of academic performance, majority (85.05%) students who had CGPA of third class at the beginning of their studies dropped out before expected years of graduation. The study recommends that government and university management provide financial support to assist the male students who are on self-sponsorship, as financial pressure may contribute to their dropout rates

Examining the Drivers and Boundary Conditions of Social Innovation: Evidence from MNE Subsidiaries in a Developing Economy

Although social innovation can help multinational enterprise (MNE) subsidiaries create social value for developing countries, they often encounter significant challenges in successfully implementing social innovation projects. This research applies the knowledge-based perspective to propose and test a theoretical framework to explain why MNE subsidiaries differ in their ability to pursue social innovation successfully in a developing country. The framework contends that MNEs’ relationship learning contributes to social innovation variability under varying levels of subsidiary autonomy and mode of entry. Analysis of primary data collected from 207 subsidiaries of MNEs operating in Ghana shows that relationship learning has a positive relationship with social innovation. Further analysis reveals that subsidiary autonomy enhances the positive association between relationship learning and social innovation, and that this moderating effect is stronger for subsidiaries with equity entry mode as opposed to non-equity entry mode. These insights advance the limited understanding of the antecedents of MNEs’ social innovation in developing countries and offer guidance on how MNE subsidiaries can successfully pursue social innovation interventions in a developing country.

Creative Becoming: Response to Palmer’s “The Aesthetic Matrix”

ABSTRACT In this response to Palmer, I focus on the potential of creative spaces. In resonance with Palmer’s evocative descriptions of his engagement with painting and its relation to clinical insight, I offer my parallel experience with dance. Immersive experiences in creative endeavors offer to a clinician a mode of intuitive entry into the analytic field of a given treatment. I discuss the need to facilitate, and to hold open, a space for creativity for our patients and for ourselves. Creating connects us – to our self, to others, to internal objects, to our mother-child origins and to the aesthetic pleasures of our first communications. The aesthetic capacity to keep this embodied vitality alive in the analytic relationship is the quality I conceptualize as analytic eroticism. Bringing a creative sensibility into our clinical work significantly informs how we inhabit the analytic field.

Megalin-mediated siRNA uptake in kidney proximal tubule cells

Small interfering RNA (siRNA) is a clinically validated therapeutic modality, which silences gene expression via RNA interference (RNAi). Systemically delivered siRNA molecules are excreted primarily via the kidneys, where they accumulate in the renal cortex. Proximal epithelial cells within the tubule are thus an attractive target cell for utilizing RNAi, where the primary mode of entry is likely endocytic uptake. Here we report using a well-differentiated, opossum kidney (OK) proximal tubule cell (PTEC) line as a model system to mimic proximal tubule biology and investigate siRNA uptake. Fluorescently tagged siRNA were employed to observed uptake, which was observed in both a time and concentration dependent manner occurring predominantly from the apical surface of OK cells. Live cell imaging revealed significant colocalization of siRNAs with LysoTracker, consistent with endocytic uptake and delivery to lysosomes. We investigated the role of key PTEC solute recovery receptors, megalin and cubilin. siRNA uptake was inhibited by the megalin-binding protein RAP; and was independently reduced in Lrp2 knock-out (KO) cell lines. This work suggests that PTECs employ megalin to facilitate uptake of siRNA via endocytosis. Additionally, we observed colocalization of siRNA molecules and megalin in mouse kidneys from in vivo studies. Elucidating the mechanism(s) for siRNA uptake in the proximal tubule aids in the development of RNAi renal therapeutics and, more broadly, the understanding of class effects associated with siRNA nephrotoxicity. Additional work is focused on intracellular traffcking and megalin mediated siRNA uptake in mice. Project funded by Judo Bio. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Association of mode of entry to a nursing programme and student success: A two-year retrospective multi-cohort study.

The issue of the mode of entry to nursing programmes and its effect on student success is a key concern among researchers globally. Identifying the mode of entry, which has the potential to improve academic success, decrease the failure rate and lead to the successful completion of a degree, is crucial to increase the nursing workforce. The objectives of this study were to assess the association of mode of entry to a nursing programme on student success among undergraduate students. The study was conducted at a public nursing education institution (NEIs) in the northeast of Namibia. A retrospective multi-cohort study was conducted to assess the association of mode of entry on student success. Academic outcomes were analysed, observing two cohorts of nursing students. Results show that 76.2% (f = 16) of mature-age entry students and 53.7% (f = 29) of direct entry students completed their programme on time. However, 42.1% (f = 8) of access students were expected to return. Female students 56.8% (f = 25) and male students 56.0% (f = 28) completed on time. There was no significant relationship between the mode of entry and completion status with p > 0.05, respectively. A higher percentage of mature-age entry students was found to complete their studies on time than direct entry and English access entry students. These findings could be used in the revision of student recruitment strategies to select nursing students who are more likely to achieve the best academic outcomes.

Navigating Global Markets: Insights into Ceramic Firm Performance and Market Entry Strategies

Analyzing the intersection of market entry strategies and ceramic firm performance in the international market, this study offers insights into the dynamics shaping the competitiveness of U.S. ceramic firms abroad. By examining the strategic decisions guiding market selection and entry modes, this research sheds light on the factors influencing the success of ceramic enterprises on the global stage. This article delves into the intricacies of market entry strategies adopted by U.S. ceramic firms as they venture into international markets. It explores the strategic decisions made regarding market selection, distinguishing between English- and non-English-speaking countries, and the various modes of entry employed, including exporting, licensing, joint ventures, subsidiaries, and retail stores. The findings from a comprehensive analysis of 57 firms engaged in international markets unveil a significant trend: firms targeting culturally similar markets, particularly English-speaking ones, tend to achieve superior revenue generation and overall performance compared to those entering non-English-speaking countries. Additionally, through a cluster analysis, the study identifies four overarching modes of entry ranging from straightforward direct exporting to more intricate strategies integrating subsidiaries and retail outlets abroad. Surprisingly, the mode of entry doesn't exhibit a clear association with performance outcomes. This suggests a cautious approach by U.S. ceramic firms, opting for markets with lower levels of uncertainty, primarily through export activities.

The development of an egg-soaking method for delivering dsRNAs into spider mites

Recently, the first sprayable RNAi biopesticide, Ledprona, against the Colorado potato beetle, Leptinotarsa decemlineata, has been registered at the United States Environmental Protection Agency. Spider mites (Acari: Tetranychidae), a group of destructive agricultural and horticultural pests, are notorious for rapid development of insecticide/acaricide resistance. The management options, on the other hand, are extremely limited. RNAi-based biopesticides offer a promising control alternative to address this emerging issue. In this study, we i) developed an egg-soaking dsRNA delivery method; ii) evaluated the factors influencing RNAi efficiency, and finally iii) investigated the potential mode of entry of this newly developed egg-soaking RNAi method. In comparison to other dsRNA delivery methods, egg-soaking method was the most efficient, convenient/practical, and cost-effective method for delivering dsRNAs into spider mites. RNAi efficiency of this RNAi method was affected by target genes, dsRNA concentration, developmental stages, and mite species. In general, the hawthorn spider mite, Amphitetranychus viennensis, is more sensitive to RNAi than the two-spotted spider mite, Tetranychus urticae, and both of them have dose-dependent RNAi effect. For different life stages, egg and larvae are the most sensitive life stages to dsRNAs. For different target genes, there is no apparent association between the suppression level and the resultant phenotype. Finally, we demonstrated that this egg-soaking RNAi method acts as both stomach and contact toxicity. Our combined results demonstrate the effectiveness of a topically applied dsRNA delivery method, and the potential of a spray induced gene silencing (SIGS) method as a control alternative for spider mites.

Trading wage jobs for dreams: the interplay between entry modes into self-employment and the duration of subsequent self-employment stints

PurposeWage employees enter self-employment either directly or in a staged manner and may subsequently undertake multiple stints at self-employment. Extant research on the relationship between entry modes and the persistence and outcomes of self-employment is inconclusive. This study investigates the relationship between wage employees’ initial mode of entry into self-employment and the duration of the subsequent first two stints of self-employment.Design/methodology/approachThis study used a matched longitudinal sample of 9,550 employees who became majority owners of incorporated firms from 2005 to 2016.FindingsThe findings demonstrate that the initial mode of entry into self-employment matters for the first two stints at self-employment. Staged entry into self-employment was associated with a shorter first stint and became insignificant for the second stint. Staged entry into self-employment was positively related to the odds of becoming self-employed for the second time in the same firm.Originality/valueUsing a comprehensive and reliable dataset, the paper shifts focus from the aggregated onward journey of novice entrepreneurs (survival as the outcome) to the duration of their self-employment stints. By doing so, the paper offers insights into the process of becoming self-employed and the patterns associated with success/failure in entrepreneurship associated with self-employment duration.

Motivational factors for choosing dentistry as a career: A survey of undergraduate dental students in Ireland

Introduction Dentistry is a highly sought after university course but the motivations for students choosing dentistry as a career in Ireland are not well understood. Objective The aim of this study is to investigate the reasons why students choose dentistry as a professional career in Ireland. Methods All undergraduate dental students (n=225) in an Irish university in the academic year 2016/17 were invited to participate in a cross-sectional survey. Participants were asked to rate a list of 12 factors that influenced their decision to pursue dentistry as a career. Results The response rate was 85.3% (n=192). The majority of the participants were female (65%), aged between 18 and 23 years (69%) and from the European Union (60%). Over half (55%) entered the programme directly from secondary-level education. The motivational factor with the highest median rating of importance was altruism. Conclusion Motivational factors influencing dental students included altruism, financial security, and independence. Having a previous family connection with the dental profession did not feature strongly. Positive previous experiences of dentistry and dental professionals emerged as a new motivational factor. Nationality, mode of entry and year of study influenced the motivational factors of the students surveyed.