The following paper examines the determinants of transport mode choice for trips in the Metropolitan Area of the Mexican Valley. Using a data set from the Mexican household travel survey (Encuesta Origen - Destino) conducted in 2017 and multinomial logistic regression, the influence of transportation supply, spatial characteristics and socio-economic factors on individual mode choice is estimated. To our knowledge, this is the first analysis of mobility behaviour in the Metropolitan Area of the Mexican Valley with this newly developed data set.The main findings show that a better Mass Rapid Transit infrastructure is not consistently positively associated with the likelihood of using this service. While a better road infrastructure is promoting the use of Mass Rapid Transit services over driving, it is negatively linked with the likelihood of walking and bicycling. These slow modes are mainly interfered with the distance to the city centre: the further away from the centre, the higher the likelihood of walking and bicycling instead of using the car.With respect to socio-economic characteristics of individuals, women and the young found to have a higher likelihood of using public transport than the car. In contrast, having children is negatively associated with the likelihood of using public transport. Moreover, a higher education of individuals is found to be positively associated with car use, while car availability is negatively associated with all other transport mode options.