This study involves a comparative analysis of two waste substrates; cow dung and poultry droppings. The purpose of this study was to ascertain which of the waste substrates produces a greater yield of biogas, their individual retention period as well as the percentage of the methane content in the gas generated. The study was divided into two experiments which comprised of a water displacement set up in a laboratory and one 30 L fixed dome digester for each waste substrate. In both experiments waste substrates were mixed with water in ratio 1:1 and operated at a mesophilic temperature condition. In the laboratory, the daily gas yield of the individual substrates in ml was obtained, it was observed that cow dung produced an average of 29.9 ml of biogas per day and the poultry waste produced 60.7 ml per day. A gas analysis was performed to obtain the gas profile of gas produced from the two substrates. As deduced from the analysis, cow dung had a methane and CO2 weight percentage of about 92 wt% and 6.68 wt% respectively, while poultry droppings had about 90 wt% methane and 6.56 wt% CO2.With the aid of a constructed mini gas stove, a flame test was performed, which gave off a blue flame. Hence, the two substrates are efficient for biogas production.