Mixture Of Gelatin Research Articles

Colorimetric detection of Aflatoxin B1 by using smartphone-assisted microfluidic paper-based analytical devices

Aflatoxin B 1 (AFB 1 ) is a powerful carcinogen, and the pollution it causes has become a global public health safety issue. Therefore, microfluidic paper-based analytical devices (μPADs) with smartphones to read colorimetric signals were constructed for rapid AFB 1 detection. The colorimetric mechanism of μPADs was based on the common starch–iodine reaction, and the addition of gelatin/chitosan enhanced the colorimetric effect. During detection and analysis, the competitive analysis occurred in the process of AFB 1 monoclonal antibody's recognition of free AFB 1 and functionalized antigen. The glucose oxidase-labeled IgG was used as the secondary antibody. Hydrogen peroxide, an oxidation product of the glucose, caused a chroma change in μPADs. Simultaneously, the change in chroma was consistent with the change in AFB 1 concentration. Under the best optimized conditions, the limits of detection and quantitation of AFB 1 in the buffer were 9.45 and 12.00 ng mL −1 , respectively. A comparison of the detection performance of commercial kits with that of the method introduced herein confirmed the superiority of our method in actual food samples with known AFB 1 concentrations. This colorimetric μPADs analysis met the detection requirements of some countries, such as China, and had great potential for development in the direction of daily extensive food safety control and environmental monitoring. • Portable and low-cost smartphone-based microfluidic paper-based analytical devices (μPADs). • The colorimetric μPAD detection system can detect aflatoxin B1 as low as 9.45 ng mL −1 through Lab data analysis. • The colorimetric mechanism is based on the common starch–iodine reaction. • The addition of gelatin and chitosan mixture enhances the colorimetric effect.

Constructing the Mo2C@MoOx Heterostructure for Improved SERS Application.

Surface-enhanced Raman scattering (SERS) is a non-destructive spectra analysis technique. It has the virtues of high detectivity and sensitivity, and has been extensively studied for low-trace molecule detection. Presently, a non-noble-metal-based SERS substrate with excellent enhancement capabilities and environmental stability is available for performing advanced biomolecule detection. Herein, a type of molybdenum carbide/molybdenum oxide (Mo2C@MoOx) heterostructure is constructed, and attractive SERS performance is achieved through the promotion of the charge transfer. Experimentally, Mo2C was first prepared by calcinating the ammonium molybdate tetrahydrate and gelatin mixture in an argon atmosphere. Then, the obtained Mo2C was further annealed in the air to obtain the Mo2C@MoOx heterostructure. The SERS performance was evaluated by using a 532 nm laser as an excitation source and a rhodamine 6G (R6G) molecule as the Raman reporter. This process demonstrates that attractive SERS performance with a Raman enhancement factor (EF) of 1.445 × 108 (R6G@10−8 M) and a limit of detection of 10−8 M can be achieved. Furthermore, the mechanism of SERS performance improvement with the Mo2C@MoOx is also investigated. HRTEM detection and XPS spectra reveal that part of the Mo2C is oxidized into MoOx during the air-annealing process, and generates metal–semiconductor mixing energy bands in the heterojunction. Under the Raman laser irradiation, considerable hole–electron pairs are generated in the heterojunction, and then the hot electrons move towards MoOx and subsequently transfer to the molecules, which ultimately boosts the Raman signal intensity.

The pH-responsive phase separation of type-A gelatin and dextran characterized with static multiple light scattering (S-MLS)

Macromolecular phase separation has attracted increasing attention due to its wide applications in food industry, such as modulating food texture and delivering bioactive compounds. The phase separation kinetics is important but rarely reported due to the limitation of current methods. This work provided an effective method, static multiple light scattering (S-MLS), for the study of phase separation kinetics, and investigated the effects of pH on the phase separation of type-A gelatin and dextran mixtures (GE/DE) using S-MLS technique, fluorescence microscopy and visual assessment. Phase diagrams of GE/DE (1.0–6.0 wt%) at pH 3.00–11.0 were measured based on the micro-phase separation. Interestingly, GE/DE mixtures were likely to be miscible at low and high pHs. GE/DE (4.0 wt%/4.0 wt%) mixture at pH 3.00–10.0 was selected for following study. Phase separation of GE/DE (4.0 wt%/4.0 wt%) mixture was pH-responsive, e.g. no phase separation at pH 3.00–4.75 and pH 10.0, only microphase separation at pH 5.00 and 5.25, and both micro- and macro-phase separation at pH 5.50–9.00. The order of phase separation rates was pH5.50 > pH6.00 > pH9.00 > pH7.00 > pH8.00. Based on the results of S-MLS, micro- and macro-phase separation images, phase separation process was proposed to be divided into four steps, including droplet coalescence, droplet migration, primary and stable phase separation. The variation of the GE/DE interaction and the self-aggregation of GE as a function of the pH might explain the pH-response of GE/DE phase separation. S-MLS is an effective method to study the phase separation kinetics of macromolecular systems.

Multifunctional electrospun asymmetric wettable membrane containing black phosphorus/Rg1 for enhancing infected wound healing.

Bacterial infection is one of the most frequent complications in the burn and chronic wounds. Inspired by natural existing superhydrophobic surface structures, a novel asymmetric wettable membrane was prepared using the electrospinning technique for facilitating the bacteria‐infected wound healing. Herein, the prepared membrane consists of two layers: The hydrophobic outer layer was composed of poly (lactic‐co‐glycolic) acid (PLGA) and black phosphorus‐grafted chitosan (HACC‐BP), while the hydrophilic inner layer was composed by using a mixture of gelatin (Gel) with ginsenoside Rg1 (Rg1). Biological studies in vitro showed BP@PLGA/Gel (BP@BM) membrane with excellent antibacterial activity could significantly inhibit the adhesion of bacteria, and Rg1 could facilitate the migration and tube formation of human umbilical vein endothelial cells (HUVECs). Compared to Aquacel Ag dressing, the result in vivo revealed that the Rg1/BP@BM could facilitate better wound healing by triggering phosphoinositide 3‐kinase (P‐PI3K/PI3K) and phosphorylation of protein kinase B (P‐AKT/AKT) signaling pathways, upregulating Ki67, CD31, α‐SMA, and TGF‐β1, and downregulating TNF‐α, IL‐1β, and IL‐6, promoting M2 polarization (IL‐10, CD206, and Arg‐1) of macrophages, inhibiting M1 polarization (iNOS) of macrophages. These findings suggested that the asymmetric wettable membrane have the huge potential for wound healing.

Characterization of Sustainable Robotic Materials and Finite Element Analysis of Soft Actuators Under Biodegradation.

Biodegradability is an important property for soft robots that makes them environmentally friendly. Many biodegradable materials have natural origins, and creating robots using these materials ensures sustainability. Hence, researchers have fabricated biodegradable soft actuators of various materials. During microbial degradation, the mechanical properties of biodegradable materials change; these cause changes in the behaviors of the actuators depending on the progression of degradation, where the outputs do not always remain the same against identical inputs. Therefore, to achieve appropriate operation with biodegradable soft actuators and robots, it is necessary to reflect the changes in the material properties in their design and control. However, there is a lack of insight on how biodegradable actuators change their actuation characteristics and how to identify them. In this study, we build and validate a framework that clarifies changes in the mechanical properties of biodegradable materials; further, it allows prediction of the actuation characteristics of degraded soft actuators through simulations incorporating the properties of the materials as functions of the degradation rates. As a biodegradable material, we use a mixture of gelatin and glycerol, which is fabricated in the form of a pneumatic soft actuator. The experimental results show that the actuation performance of the physical actuator reduces with the progression of biodegradation. The experimental data and simulations are in good agreement (R 2 value up to 0.997), thus illustrating the applicability of our framework for designing and controlling biodegradable soft actuators and robots.

Bioactive coating for tissue-engineered smalldiameter vascular grafts

Objective: to develop a method for modifying composite small-diameter porous tubular biopolymer scaffolds based on bacterial copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and gelatin modified with a double-layered bioactive coating based on heparin (Hp) and platelet lysate (PL) that promote adhesion and proliferation of cell cultures.Materials and methods. Composite porous tubular biopolymer scaffolds with 4 mm internal diameter were made by electrospinning from a 1 : 2 (by volume) mixture of a 10% solution of poly(3-hydroxybutyrateco- 3-hydroxyvalerate) copolymer, commonly known as PHBV, and a 10% solution of gelatin, respectively, in hexafluoro-2-propanol. The structure of the scaffolds was stabilized with glutaraldehyde vapor. The scaffolds were modified with a bioactive Hp + PL-based coating. The surface morphology of the samples was analyzed using scanning electron microscopy. Biological safety of the modified scaffolds in vitro (hemolysis, cytotoxicity) was evaluated based on the GOST ISO 10993 standard. Interaction with cultures of human endothelial cell line (EA. hy926) and human adipose-derived mesenchymal stem cells (hADMSCs) was studied using vital dyes.Results. We developed a method for modifying small-diameter composite porous tubular biopolymer scaffolds obtained by electrospinning from a mixture of PHBV and gelatin modified with double-layered bioactive coating based on covalently immobilized Hp and human PL. The modified scaffold was shown to have no cytotoxicity and hemolytic activity in vitro. It was also demonstrated that the developed coating promotes hADMSC adhesion and proliferation on the external surface and EA.hy926 on the internal surface of the composite porous tubular biopolymer scaffolds in vitro.Conclusion. The developed coating can be used for the formation of in vivo tissueengineered small-diameter vascular grafts.

Study on Influence of Use of Waste Cooking and Engine Oil on the Properties of Bituminous Concrete

Abstract: Bitumen is defined as a gelatinous viscid mixture of hydrocarbons attained naturally or as a residue from petroleum refinement which is used for pavement materialization and roofing. Bitumen is employed as a binder for flexible pavements throughout the globe. Though bitumen is non-hazardous under normal conditions but when heated it becomes toxic and has consequences of environmental degradation. Also, bitumen being a product of non-renewable source of energy i.e. petroleum will led to depletion of petroleum reserves. It is a key challenge in highway industry to scale back the dependence on fossil fuels & to recycle the highway waste. The asphalt industry is undoubtedly a sector that contains a sustainable environmental impact, one amongst the main component being binder, bitumen, which is produced from petroleum. Bitumen generation results in enormous amounts of carbon dioxide emission which causes hazardous environmental impact. This research work is about the employment of waste oils as the alternative binders. The waste oils employed are waste cooking and waste engine oil. These are studied and analyzed as a step towards sustainable environment. This project work will provide an alternative or modified binder as well as will serve with the better way for safe disposal of waste oils generated. Thus, this project is beneficial concerning both the environmental aspects of alternative binder and safe disposal of waste oils. Keywords: Pavements, Bitumen, Engine Oil, Cooking Oil, Addition Percentage, Highway Industry.

Design of a 3D printed coronary artery model for CT optimization

IntroductionTo design a custom phantom of the coronary arteries to optimize CT coronary angiography (CCTA) protocols. MethodsCharacteristics of the left and right coronary arteries (mean Hounsfield Unit (HU) values and diameters) were collected from consecutive CCTA examinations (n = 43). Four different materials (two mixtures of glycerine, gelatine and water, pig hearts, Ecoflex™ silicone) were scanned inside a Lungman phantom using the CCTA protocol to find the closest model to in vivo data. A 3D printed model of the coronary artery tree was created using CCTA data by exporting a CT volume rendering into Autodesk Meshmixer™ software. The model was placed in an acid bath for 5 h, then covered in Ecoflex™, which was removed after drying. Both the Ecoflex™ and pig heart were later filled with a mixture of contrast (Visipaque 320 mg I/ml), NaCl and gelatin and scanned with different levels of tube current and iterative reconstruction (ASiR-V). Objective (HU, noise and size (vessel diameter) and subjective analysis were performed on all scans. ResultsThe gelatine mixtures had HU values of 130 and 129, Ecoflex™ 65 and the pig heart 56. At the different mA/ASiR-V levels the contrast filled Ecoflex™ had a mean HU 318 ± 4, noise 47±7HU and diameter of 4.4 mm. The pig heart had a mean HU of 209 ± 5, noise 38±4HU and a diameter of 4.4 mm. With increasing iterative reconstruction level the visualisation of the pig heart arteries decreased so no measurements could be performed. ConclusionThe use of a 3D printed model of the arteries and casting with the Ecoflex™ silicone is the most suitable solution for a custom-designed phantom. Implications for practiceCustom designed phantoms using 3D printing technology enable cost effective optimisation of CT protocols.

Seed Mucilage: Biological Functions and Potential Applications in Biotechnology.

In plants, the diaspore (seed dispersal unit) may include a seed coat and/or pericarp to protect the embryo and assist in dispersion. In many species, the seed coat and/or pericarp secrete a gelatinous mixture of cell wall polysaccharides known as mucilage. In several species, mucilage synthesis, secretion and modification have been studied extensively as model systems for the investigation of plant cell wall structure and function. Despite this, efforts toward understanding the role of mucilage have received less attention. Mucilage has been hypothesized to impact seed dispersal through interaction with soil, protecting the seed in the gut following ingestion by animals or affecting the ability of seeds to sink or float in water. Mucilage has been found to influence seed germination and seedling establishment, most often during abiotic stress, probably by maintaining seed hydration when water is scarce. Finally, mucilage has been documented to mediate interactions with various organisms. Advances in transgenic technology should enable the genetic modification of mucilage structure and function in crop plants. Cells synthesizing mucilage may also be a suitable platform for creating custom polysaccharides or proteins with industrial applications. Thus, in the near future, it is likely that research on seed mucilage will expand well beyond the current focus. Here we summarize our understanding of the biological functions of mucilage and provide an outlook on the future of mucilage research.

Bioaccessibility and antioxidant activity of phenolic acids, flavonoids, and anthocyanins of encapsulated Thai rice bran extracts during in vitro gastrointestinal digestion

Encapsulation technique was applied to improve the stability of bioactive compounds in bran extracts from Thai rice cultivars (Khao Dawk Mali 105, Kiaw Ngu, Hom Nil, and Leum Pua), using three carriers including gelatin, gum Arabic, and the mixture of gelatin and gum Arabic. The microcapsules obtained using gelatin provided a higher production yield of 76.08, 85.63, 85.63 and 85.59%, respectively. A greater encapsulation efficiency was also observed in the extracts encapsulated with gelatin (93.45, 95.91, 91.19 and 95.09%, respectively). After simulated gastric and intestinal digestion, the microcapsules formed by using gelatin exhibited the higher release of bioactive compounds and antioxidant activity than unencapsulated extracts. However, the extracts encapsulated using gelatin and gum Arabic complex yielded the lowest release of bioactive compounds and their antioxidant activity after simulated digestion. The overall results showed that gelatin was an appropriate carrier that could protect bioactive compounds from the digestion conditions.

Quantification of assembly forces during creation of head-neck taper junction considering soft tissue bearing: a biomechanical study

BackgroundAll current total hip arthroplasty (THA) systems are modular in design. Only during the operation femoral head and stem get connected by a Morse taper junction. The junction is realized by hammer blows from the surgeon. Decisive for the junction strength is the maximum force acting once in the direction of the neck axis, which is mainly influenced by the applied impulse and surrounding soft tissues. This leads to large differences in assembly forces between the surgeries. This study aimed to quantify the assembly forces of different surgeons under influence of surrounding soft tissue.MethodsFirst, a measuring system, consisting of a prosthesis and a hammer, was developed. Both components are equipped with a piezoelectric force sensor. Initially, in situ experiments on human cadavers were carried out using this system in order to determine the actual assembly forces and to characterize the influence of human soft tissues. Afterwards, an in vitro model in the form of an artificial femur (Sawbones Europe AB, Malmo, Sweden) with implanted measuring stem embedded in gelatine was developed. The gelatine mixture was chosen in such a way that assembly forces applied to the model corresponded to those in situ. A study involving 31 surgeons was carried out on the aforementioned in vitro model, in which the assembly forces were determined.ResultsA model was developed, with the influence of human soft tissues being taken into account. The assembly forces measured on the in vitro model were, on average, 2037.2 N ± 724.9 N, ranging from 822.5 N to 3835.2 N. The comparison among the surgeons showed no significant differences in sex (P = 0.09), work experience (P = 0.71) and number of THAs performed per year (P = 0.69).ConclusionsAll measured assembly forces were below 4 kN, which is recommended in the literature. This could lead to increased corrosion following fretting in the head-neck interface. In addition, there was a very wide range of assembly forces among the surgeons, although other influencing factors such as different implant sizes or materials were not taken into account. To ensure optimal assembly force, the impaction should be standardized, e.g., by using an appropriate surgical instrument.

Study on Preparation and Properties of Coaxial Electrospun PLGA/Gelatin Fibers

The coaxial electrospun fibers with large specific surface area, high porosity and core-shell structure have been great applied in biomedical field, especially as drug delivery carriers. In this paper, PLGA(polylactic acid/glycolic acid copolymer) was used as the core and the mixture of PLGA and gelatin was used as the shell. PLGA/gelatin fiber was prepared by coaxial electrospinning technology. The effects of different parameters on the surface morphology and the diameter of fibers were investigated.

Demineralized Dentin Matrix Particle-Based Bio-Ink for Patient-Specific Shaped 3D Dental Tissue Regeneration.

Demineralized dentin matrix (DDM)-based materials have been actively developed and are well-known for their excellent performance in dental tissue regeneration. However, DDM-based bio-ink suitable for fabrication of engineered dental tissues that are patient-specific in terms of shape and size, has not yet been developed. In this study, we developed a DDM particle-based bio-ink (DDMp bio-ink) with enhanced three-dimensional (3D) printability. The bio-ink was prepared by mixing DDM particles and a fibrinogen–gelatin mixture homogeneously. The effects of DDMp concentration on the 3D printability of the bio-ink and dental cell compatibility were investigated. As the DDMp concentration increased, the viscosity and shear thinning behavior of the bio-ink improved gradually, which led to the improvement of the ink’s 3D printability. The higher the DDMp content, the better were the printing resolution and stacking ability of the 3D printing. The printable minimum line width of 10% w/v DDMp bio-ink was approximately 252 μm, whereas the fibrinogen–gelatin mixture was approximately 363 μm. The ink’s cytocompatibility test with dental pulp stem cells (DPSCs) exhibited greater than 95% cell viability. In addition, as the DDMp concentration increased, odontogenic differentiation of DPSCs was significantly enhanced. Finally, we demonstrated that cellular constructs with 3D patient-specific shapes and clinically relevant sizes could be fabricated through co-printing of polycaprolactone and DPSC-laden DDMp bio-ink.

Water-in-water-in-water emulsions formed by cooling mixtures of guar, amylopectin and gelatin

Water-in-water emulsions were formed by mixing aqueous solutions of the neutral polysaccharides guar and amylopectin. The effect of adding gelatin B on emulsions, both of a dispersed guar rich phase in a continuous amylopectin phase and the inverse, were investigated using confocal scanning laser microscopy as a function of the gelatin concentration between 0.25 and 1.5 wt% and the pH between pH 4 and pH 6. Gelatin was found to phase separate upon cooling below about 25 °C in a narrow pH range around pH 5 both in the pure guar phase and in the pure amylopectin phase forming distinct spherical domains. The phase diagram was established as a function of the gelatin concentration and the pH. In the emulsions, the gelatin phase separated first at the interface between the two phases forming a continuous layer at the droplet surface. In some conditions the layer persisted, but in others it broke up into distinct domains. Excess gelatin phase separated in the bulk amylopectin phase, particularly when it was the continuous phase. The gelatin redispersed into the bulk phases when the emulsions were heated to 30 °C.

Thermoreversible gels – Optimisation of processing parameters in fused Deposition Modelling

Thermoreversible hydrogels are widely used in foods, drug delivery and tissue engineering. Novel shapes/textures out of them are being increasingly fabricated using Fused Deposition Modelling (FDM) type 3D printing, where gels are extruded layer-by-layer above their gelling temperature followed by cooling. It is crucial to develop a library of suitable materials and optimise printing conditions to ensure good print quality and to minimise damage to the embedded active components (cells, flavours, nutrients and drug molecules).Several mixtures of gelatin with gellan and agar were prepared and characterised. They were formed into cylinders using FDM at four different temperatures and three different speeds. The printed specimens were tested for their mechanical properties using compression.The fidelity of printed shapes and the inter-layer adhesion appeared to rely on the cooling required before approaching the gelling temperature and the time available for it, through printing speed. A generic printability diagram was constructed for navigating to the optimal printing conditions for a given formulation.

Modulating the physico-mechanical properties of polyacrylamide/gelatin hydrogels for tissue engineering application

Along with providing an environment for cell attachment and proliferation, a tissue engineering scaffolds should possess physical and mechanical properties that would fit the target tissue. The present study aimed to manipulate physico-mechanical properties of polyacrylamide/gelatin hydrogels using response surface method-central composite design (RSM-CCD) to reach a scaffold with defined properties. On this demand, mixtures of gelatin and acrylamide (AAm) monomer were used to prepare semi-interpenetrating hydrogels by free radical polymerization of AAm. Selected variables for statistical modeling were chosen to be weight ratios of monomer/crosslinker, monomer/gelatin, and monomer/initiator. The desired responses were compressive modulus, compressive strength, and swelling. Results showed that desired responses could be tailored by varying these parameters with the highest impact for monomer/crosslinker ratio. The swelling ratio of hydrogels was in the range of 947–1654%, while the modulus varied between 5 and 35 kPa. The cyclic compressive test showed the durability of hydrogels under cyclic loadings. Finally, the results of cell attachment and cytocompatibility analyses indicated that the hydrogels were completely biocompatible and enhanced cell attachment. Thus, these hydrogels could potentially be used as tissue engineering scaffolds for load-bearing organs, including muscle and cartilage, or could be used for in vitro differentiation of stem cells using mechanical clues.

Glow-in-the-Dark Patterned PET Nonwoven Using Air-Atmospheric Plasma Treatment and Vitamin B2-Derivative (FMN)

Flavin mononucleotide (FMN) derived from Vitamin B2, a bio-based fluorescent water-soluble molecule with visible yellow-green fluorescence, has been used in the scope of producing photoluminescent and glow-in-the-dark patterned polyester (PET) nonwoven panels. Since the FMN molecule cannot diffuse inside the PET fiber, screen printing, coating, and padding methods were used in an attempt to immobilize FMN molecules at the PET fiber surface of a nonwoven, using various biopolymers such as gelatin and sodium alginate as well as a water-based commercial polyacrylate. In parallel, air atmospheric plasma activation of PET nonwoven was carried for improved spreading and adhesion of FMN bearing biopolymer/polymer mixture. Effectively, the plasma treatment yielded a more hydrophilic PET nonwoven, reduction in wettability, and surface roughness of the plasma treated fiber with reduced water contact angle and increased capillary uptake were observed. The standard techniques of morphological properties were explored by a scanning electron microscope (SEM) and atomic force microscopy (AFM). Films combining each biopolymer and FMN were formed on PS (polystyrene) Petri-dishes. However, only the gelatin and polyacrylate allowed the yellow-green fluorescence of FMN molecule to be maintained on the film and PET fabric (seen under ultraviolet (UV) light). No yellow-green fluorescence of FMN was observed with sodium alginate. Thus, when the plasma-activated PET was coated with the gelatin mixture or polyacrylate bearing FMN, the intense photoluminescent yellow-green glowing polyester nonwoven panel was obtained in the presence of UV light (370 nm). Screen printing of FMN using a gelatin mixture was possible. The biopolymer exhibited appropriate viscosity and rheological behavior, thus creating a glow-in-the-dark pattern on the polyester nonwoven, with the possibility of one expression in daylight and another in darkness (in presence of UV light). A bio-based natural product such as FMN is potentially an interesting photoluminescent molecule with which textile surface pattern designers may create light-emitting textiles and interesting aesthetic expressions.

3D Printing Geometric Scaffold Design Variation of Injectable Bone Substitutes (IBS) Pa

3D printing technology application in tissue engineering could be provided by designing geometrical scaffold architecture which also functionates as drug delivery. For drug delivery scaffold on bone tuberculosis, the cell pore of the geometric design was filled with Injectable Bone Substitutes (IBS) which had streptomycin as anti-tuberculosis. In this study, scaffolds were synthesized in three cells geometric filled by Injectable Bone Substitutes (IBS), Hexahedron, Truccated Hexahedron, and Rhombicuboctahedron, which had 2.5 mm x 2.5 mm x 2.5 mm size dimension and 0.8 mm strut. The final design was printed in 3D with polylactic acid (PLA) filament using the FDM process (Fused Deposition Modelling). The composition of IBS paste was a mixture of hydroxyapatite (HA) and gelatine (GEL) 20% w/v with a ratio of 60:40, streptomycin 10 wt% and hydroxypropyl methylcellulose (HPMC) 4% w/v. It was then characterized using Fourier-transform infrared spectroscopy (FTIR). Scaffold–paste characterization was included pore size test of 3D printing result before and after injected using Scanning Electron Microscope SEM, porosity test, and compressive strength test. The result showed that the pore of scaffold design was 1379 µm and after injected with IBS paste, the pore leaving 231.04 µm of size. The scaffold with IBS paste porosity test showed ranges between 40,78-70,04% while the compressive strength of before and after injected ranges between 1,110-634 MPa and 2,217-6,971 MPa respectively. From the test results, the scaffold 3D printing with IBS paste in this study had suitable physical characteristics to be applicated on cancellous bones which were infected by tuberculosis.

Ternary nano-biocomposite films using synergistic combination of bacterial cellulose with chitosan and gelatin for tissue engineering applications

Ternary nano-biocomposite films of bacterial cellulose-chitosan-gelatin (BC-C-G) were fabricated by immersing the BC pellicles into chitosan and gelatin mixture and subsequently freeze-drying. Scanning electron microscopy (SEM) images of the nano-biocomposite films revealed the presence of interconnected pores, with fibre diameter 20-150 nm. The composite films have a porosity of 95.3%, and showed good hydrophilicity with swelling ratio of 19 ± 1.8 and in vitro degradability. X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, and thermogravimetric (TG) analysis results showed some interactions among the molecules of BC, gelatin, and chitosan within the film. The composite film offered good matrix for adhesion and proliferation of L929 fibroblasts cells as indicated by the cell attachment study, FE-SEM of cell-film constructs and cytocompatibility assay. Thus, the nano-biocomposite films of BC-C-G could be of paramount importance as tissue engineering scaffold. The “all-natural” ternary polymer composite films of BC-C-G have not been evaluated before for biomedical applications.

Polycaprolactone-gelatin nanofibers incorporated with dual antibiotic-loaded carboxyl-modified silica nanoparticles

In this study, we used electrospun polycaprolactone (PCL) or a mixture of PCL and gelatin (Gel) in a mixed acidic solvent to develop antimicrobial electrospun nanofibers. Carboxyl-modified mesoporous silica nanoparticles (CMSNs) or CMSNs loaded with antibiotic drugs polymyxin B and vancomycin (CMSNs/ABs) were mixed with the electrospinning solution in concentrations of 1%, 2.5% and 5%. The nanofibers diameter measured between 122 and138 nm. Higher concentrations of gelatin or CMSNs increased hydrophilicity and degradability of the nanofibers. CMSNs enhanced nanofibers mechanical strength. PCL/Gel nanofibers incorporated with CMSNs/ABs (2.5% and 5%) showed high antibacterial efficiency against Pseudomonas aeruginosa and Staphylococcus aureus. Also bacterial cell adhesion decreased when 2.5% and 5% of CMSNs/ABs were incorporated in PCL/Gel mats. MTT and hemolysis assays indicated excellent biocompatibility of all types of electrospun nanofibers. This study confirms that a proper mixture of PCL, gelatin and CMSNs loaded with two antibiotics could offer antimicrobial activities with high biocompatibility and biodegradability properties.