Mixture Of Fructose Research Articles

The effect of a new mixture of sugar and sugar-alcohols compared to sucrose and glucose on blood glucose increase and the possible adverse reactions: A phase I double-blind, three-way randomized cross-over clinical trial

IntroductionSeveral sweeteners are introduced to replace sucrose in the human diet. However, they had their own limitations and concerns, particularly in terms of their taste and their long-term health consequences. This study examined the effect of a new mixture of sugars and sugar alcohol on the postprandial blood glucose levels and its possible gastrointestinal (GI) adverse reactions in human adults. MethodsIn this double-blind three-way randomized clinical trial, adults (21 with type 2 diabetes and 20 healthy) received 300ml of three beverages containing 50g glucose, sucrose, and lacritose (a mixture of lactose, fructose, sucrose, and erythritol) when they were in the fasted state in a random order. Postprandial serum glucose was checked every 30min up to 2h and the gastrointestinal reactions were collected. ResultsThe mean serum glucose was significantly lower in all time points after ingestion of the lacritose for participants with type 2 diabetes compared to glucose and sucrose (P<0.05). The blood glucose levels were significantly lower in the 30th and 60th min for healthy subjects (P<0.05). Adverse GI reactions were not significant between the test beverages. ConclusionsThe ingestion of a 50g dose of lacritose containing lactose, fructose, sucrose, and erythritol, led to an improved blood glucose levels without any significant adverse effect compared to the same amount of glucose and sucrose. Studying the long-term effects of lacritose on appetite, metabolic markers and adverse reactions is recommended. The trial was registered in Iranian registry of clinical trials: IRCT2015050912571N2.

2976 Risk of Developing Non-Alcoholic Fatty Liver Disease (NAFLD) With Increased Intake of High Fructose Corn Syrup (HFCS): A Systematic Review

INTRODUCTION: Non-alcoholic fatty liver disease (NAFLD) encompasses the spectrum of fatty liver disease without significant alcohol consumption. The pathogenesis of NAFLD is complex and according to the “multiple parallel hits hypothesis,” a number of different processes may contribute to liver inflammation. High fructose corn syrup (HFCS, a mixture of fructose and glucose monosaccharides) appears to have a major role in inducing fatty liver by both stimulating de novo lipogenesis and blocking β-fatty acid oxidation. We performed a systematic review to demonstrate the association of HFCS in NAFLD. METHODS: Two independent authors performed a thorough literature search of English language studies in PubMed, Embase, Medline, Google Scholar, and Science Citation Index to identify relevant studies between January 2010 to January 2018. Keywords used were “high fructose corn syrup,” “nonalcoholic fatty liver disease,” “nonalcoholic steatohepatitis,” “high fructose diet,” “fatty liver,” “hepatic metabolism,” “hepatic triglycerides,” “hepatic de novo lipogenesis” and “liver enzymes.” Effects of HFCS on changes in liver enzymes, hepatic metabolism, and risk of NAFLD were assessed. The strength of evidence was evaluated by considering precision, the risk of bias and consistency. RESULTS: Based on our search criteria 6 animal studies were identified and 3 were excluded after careful review. 3 animal (rat/mice) studies were included for our final analysis. We found a significant evidence from all three studies that consumption of HFCS diet increased de novo lipogenesis in association with up-regulation of fatty acid synthase, sterol regulatory element binding protein (SREBP)-1c and decreased β-oxidation with down-regulation of Peroxisome proliferator-activated receptor (PPARα) in the liver of rats. In addition, ALT (Alanine Aminotransferase) elevation and increased liver triglycerides content were observed in all the three studies. CONCLUSION: Our systematic review shows that HFCS intake is associated with increased risk of developing NAFLD. However, we need more trials looking at the association between HFCS intake and liver metabolism in the future.

Influence of sucrose substitutes and agglomeration on volatile compounds in powdered cocoa beverages

Volatile aromatic substances are the main factors contributing to the acceptability of cocoa products. The beneficial effect of fat-free ingredients of cocoa beans on human health has been scientifically proven. This encourages the consumption of cocoa products as well as further research on improving their processing technology. The aim of this study was to analyse changes in the composition of volatile compounds and their impact on the sensory characteristics of an agglomerated cocoa powder mixture with modified composition for the raw material. The basic mixture was composed of 20% cocoa and 80% sucrose. Changes in mixture composition involved partial or total replacement of sucrose with maltodextrin or a mixture of glucose and fructose. Mixing and agglomeration were carried out in a fluid bed agglomerator. The analysis of volatile compounds was carried out using a gas chromatograph coupled with mass spectrometer, and 1,2-dichlorobenzene was used as an internal standard. The analysis showed the presence of over 70 various chemical compounds. Such volatile compounds as acetic acid, 2,3-butanediol, nonanal, and pentanoic acid, were found in almost all tested products. The highest content of acetic acid was determined in cocoa powder. In the case of the investigated cocoa beverages, the raw material composition and agglomeration affected their volatile compounds content. The analyses demonstrated a reduction in the content of volatile compounds caused by agglomeration.

High-Carbohydrate Diets Affect Accumulation of Lipofuscin-Like Pigment in the Kidneys of Mice and Rats: Autofluorescence Confocal Microscopy Analysis.

The accumulation of lipofuscin-like granules in liver, kidneys, and spleen cells in mice and rats of different lines receiving 30% sugar solutions (fructose, glucose, their mixture, and sucrose) in addition to balanced semisynthetic diet for 62 or 122 days was studied by the method of laser scanning confocal microscopy. The granules were detected by their autofluorescence at maximum λex =570-600 nm and λex=488 nm. In the kidneys of rats receiving glucose and, especially, the mixture of glucose and fructose, significant accumulation of lipofuscin-like granules was found that was absent in the control group animals receiving water. Intensive accumulation of the granules was observed in the kidneys of all groups of mice receiving sugars (except for glucose). Lipofuscin-like granules were located in the cytoplasm of epithelial cells of the distal and proximal convoluted tubules. In the liver of rats and mice, the signs of accumulation of lipofuscin-like granules were absent or minimal. In rat spleen, lipofuscinlike granules were found in the red pulp in all groups, but their accumulation significantly increased in animals receiving the diet enriched with glucose and sucrose.

FRUIT SWEETNESS CHARACTERIZATION USING IMPEDANCE SPECTROSCOPY METHOD

This paper presents fruit sweetness characterization using impedance spectroscopy method. Agricultural product processing requires technology implementation to increase its production. The fruit sweetness component is composed of a mixture of fructose, glucose, and sucrose. The measurements of these molecules are usually done with a four-point probe and use DC (direct current). In this study, a fruit sweetness characterization using impedance spectroscopy method has been developed, which uses an AC (alternating current). By using this method, the fruit sweetness level can be characterized based on the impedance measurement on the flesh of fruit. The obtained characterization results show the fruit sweetness level; it can be measured by the impedance measurement. This method could characterize the fruit sweetness well and agrees with the calibration result.

Single cell oil and ethanol production by the oleaginous yeast Trichosporon fermentans utilizing dried sweet sorghum stalks

The ability of the oleaginous yeast Trichosporon fermentans to efficiently produce lipids when cultivated in dried sweet sorghum was evaluated. First, lipid production was evaluated in synthetic media mimicking the composition of sweet sorghum stalks and optimized based on the nitrogen source and C: N ratio. Under optimum conditions, the lipid production reached 3.66 g/L with 21.91% w/w lipid content by using a mixture of sucrose, glucose and fructose and peptone at C: N ratio 160. Cultivation on pre-saccharified sweet sorghum stalks offered 1.97 g/L, while it was found that sweet sorghum stalks can support yeast growth and lipid production without the need for external nitrogen source addition. At an attempt to increase the carbon source concentration for optimizing lipid production, the Crabtree effect was observed in T. fermentans. To this end, the yeast was evaluated for its potential to produce ethanol under anaerobic conditions in synthetic media and sweet sorghum. The ethanol concentration at 100 g/L glucose was 40.31 g/L, while utilizing sweet sorghum by adding a distinct saccharification step and external nitrogen source offered ethanol concentration equal to 23.5 g/L. To the authors’ knowledge, this is the first time that the Crabtree effect is observed in T. fermentans.

Fructose co-ingestion to increase carbohydrate availability in athletes.

Carbohydrate availability is important to maximize endurance performance during prolonged bouts of moderate‐ to high‐intensity exercise as well as for acute post‐exercise recovery. The primary form of carbohydrates that are typically ingested during and after exercise are glucose (polymers). However, intestinal glucose absorption can be limited by the capacity of the intestinal glucose transport system (SGLT1). Intestinal fructose uptake is not regulated by the same transport system, as it largely depends on GLUT5 as opposed to SGLT1 transporters. Combining the intake of glucose plus fructose can further increase total exogenous carbohydrate availability and, as such, allow higher exogenous carbohydrate oxidation rates. Ingesting a mixture of both glucose and fructose can improve endurance exercise performance compared to equivalent amounts of glucose (polymers) only. Fructose co‐ingestion can also accelerate post‐exercise (liver) glycogen repletion rates, which may be relevant when rapid (<24 h) recovery is required. Furthermore, fructose co‐ingestion can lower gastrointestinal distress when relatively large amounts of carbohydrate (>1.2 g/kg/h) are ingested during post‐exercise recovery. In conclusion, combined ingestion of fructose with glucose may be preferred over the ingestion of glucose (polymers) only to help trained athletes maximize endurance performance during prolonged moderate‐ to high‐intensity exercise sessions and accelerate post‐exercise (liver) glycogen repletion.

The effect of a new mixture of sugar and sugar-alcohols compared to sucrose and glucose on blood glucose increase and the possible adverse reactions: A phase I double-blind, three-way randomized cross-over clinical trial

IntroductionSeveral sweeteners are introduced to replace sucrose in the human diet. However, they had their own limitations and concerns, particularly in terms of their taste and their long-term health consequences. This study examined the effect of a new mixture of sugars and sugar alcohol on the postprandial blood glucose levels and its possible gastrointestinal (GI) adverse reactions in human adults. MethodsIn this double-blind three-way randomized clinical trial, adults (21 with type 2 diabetes and 20 healthy) received 300ml of three beverages containing 50g glucose, sucrose, and lacritose (a mixture of lactose, fructose, sucrose, and erythritol) when they were in the fasted state in a random order. Postprandial serum glucose was checked every 30min up to 2h and the gastrointestinal reactions were collected. ResultsThe mean serum glucose was significantly lower in all time points after ingestion of the lacritose for participants with type 2 diabetes compared to glucose and sucrose (P<0.05). The blood glucose levels were significantly lower in the 30th and 60th min for healthy subjects (P<0.05). Adverse GI reactions were not significant between the test beverages. ConclusionsThe ingestion of a 50g dose of lacritose containing lactose, fructose, sucrose, and erythritol, led to an improved blood glucose levels without any significant adverse effect compared to the same amount of glucose and sucrose. Studying the long-term effects of lacritose on appetite, metabolic markers and adverse reactions is recommended. The trial was registered in Iranian registry of clinical trials: IRCT2015050912571N2.

Citric acid production from the integration of Spanish-style green olive processing wastewaters with white grape pomace by Aspergillus niger

This study aimed to optimize an integrated simple process for citric acid production using Spanish-style green olive processing wastewaters enriched with sugars from white grape pomace and the robust Aspergillus niger B60. Mild mixing of equal quantities of the above streams governed satisfactory amount of appropriate carbon sources (equimolar mixture of glucose and fructose, 111.5 g/L) in the sugar-enriched wastewater and its neutralization. Various nutrients and fermentation conditions were investigated and maximum citric acid content (85 g/L) and yield (0.56 g/g) were obtained in liquid surface culture after minimum regulation by adding sucrose and NH4NO3 (100 g/L and 1.1 g/L, respectively). Scale-up experiments (5 L-scale) verified findings from small scale (250 mL). The chemical oxygen demand value and phenolic content of the treated wastewater were reduced by 78% and 64%, respectively. Findings support the potential for clustering the respective enterprises in a biorefinery plant for citric acid fermentation.

Разработка технологии производства кондитерских изделий функционального назначения

The development of new functional foods that contribute to the preservation and improvement of human health is one of the priorities in the food industry. In this regard, the production and sales of sugar confectionery products with low energy value and various enriching additives are gradually increasing, including those meant for diabetic therapeutic and dietary preventive nutrition. At the same time, the share of these products remains insignificant. The research prospective was to develop a technology for the production of marshmallow, which reduces the risk of obesity and type II diabetes. The study featured three samples of marshmallow: with granulated sugar, fructose, and inulin. The research determined the functional properties of the finished product. Three female volunteers (21, 32, and 45 years old) participated in the experiment. It was established that the replacement of granulated sugar with a natural sweetener, namely fructose and a mixture of fructose and inulin, led to a decrease in the body’s hyperglycemic response half an hour after consuming the analyzed sample, and resulted in a smooth sugar curve. Thus, natural additives and biologically active substances make sugar confectionery products functional, which is a priority for the development of the confectionery industry.

Analysis of Water, Ethanol, and Fructose Mixtures Using Nondestructive Resonant Spectroscopy of Mechanical Vibrations and a Grouping Genetic Algorithm

A new haptic sensor that is based on vibration produced by mechanical excitation from a clock coupled to a resonant cavity is presented. This sensor is intended to determine the chemical composition of liquid mixtures in a completely non-destructive method. In this case, a set of 23 samples of water, ethanol, and fructose mixtures has been used to simulate different kinds of alcoholic beverage. The spectral information from the vibrational absorption bands of liquid samples is analyzed by a Grouping Genetic Algorithm. An Extreme Learning Machine implements the fitness function that is able to classify the mixtures according to the concentration of ethanol and fructose. The 23 samples range from 0%–13% by volume of ethanol and from 0–3 g/L of fructose, all of them with different concentration. The new technique achieves an average classification accuracy of 96%.

Fabrication of a Magnet-Assembled Alignment Device for the Amperometric Detection of Carbohydrates in Combination with CE

The article describes the preparation of a magnet-assembled alignment device for the amperometric detection of carbohydrates in combination with capillary electrophoresis (CE). It consists of a self-positioning copper disc detection electrode plate, an NdFeB magnet, a painted iron screw nut as well as a cap bearing a grounding electrode, an auxiliary electrode and a reference electrode. Coaxially aligned copper wire and stainless steel tube were enclosed between two poly(methyl methacrylate) plates by far infrared-assisted encapsulation to fabricate the self-positioning electrode plate. The performance and advantages of the alignment device were demonstrated by the separation and detection of standard mixtures of mannitol, sucrose, glucose and fructose by CE. The four carbohydrates were well separated within 13 min in a 40-cm long capillary at a separation voltage of 9 kV using a 75 mM NaOH aqueous solution. Good linearity was observed between the peak current and concentration of carbohydrates over three orders of magnitude with detection limits (S/N = 3) ranging from 0.7 to 0.9 µM. The present system exhibited long-term stability and reproducibility with relative standard deviations of less than 5% for peak currents (n = 15). The advantages of the magnet-assembled alignment device include its simple design and construction, high alignment reproducibility, ease of operation, high degree of integration, portability and low cost. The method has been successfully applied in the determination of carbohydrates in Zingiber officinale Roscoe. Coaxially aligned copper wire and stainless steel guiding tube were enclosed between two PMMA plates by far infrared-assisted encapsulation to prepare self-positioning copper disc detection plate that was sandwiched between a magnet and a painted iron screw nut to form magnet-assembled alignment device for the amperometric detection of capillary electrophoresis.

Interspecific Differences in Behavioral Responses and Neuromotorics between Laboratory Rodents Receiving Rations with Easily Digested Carbohydrates.

We assessed the effect of intake of easily digested carbohydrates for 133 days on quantitative parameters of neuromotorics and cognitive function in Wistar rats and C57Bl/6J mice. Neuromotorics (muscle tone) was assessed in rats and mice by the forelimb muscle force (grip strength) over 4 months. Anxiety was assessed in the elevated plus-maze test and cognitive function (short-term and long-term memory) was evaluated by conditioned passive avoidance response (CPAR) test over 3 months. The mice, in contrast to rats, receiving the diet with easily digested sugars demonstrated suppression of neuromotorics. Anxiety increased with age in female mice, but not in rats, irrespective of the diet. Cognitive function in rats receiving experimental rations did not change significantly in comparison with the control. In mice, consumption of equimolar mixture of fructose and glucose impared short-term, but not long-term memory, in comparison with the group receiving glucose alone. We revealed a small (by 14-17%), but statistically significant increase in the brain weight in mice receiving fructose and sucrose. The study demonstrates sufficient interspecies differences in the influence of carbohydrate rations on neuromotorics and behavioral responses in the in vivo metabolic syndrome model.

Comparison of the thermal stabilization of proteins by oligosaccharides and monosaccharide mixtures: Measurement and analysis in the context of excluded volume theory

The thermal stability of apo α–lactalbumin (α–LA) and lysozyme was measured in the presence of mixtures of glucose, fructose, and galactose. Mixtures of these monosaccharides in the appropriate stoichiometric ratio were found to have a greater stabilizing effect on each of the two proteins than equal weight/volume concentrations of di- tri- and tetrasaccharides with identical subunit composition (sucrose, trehalose, raffinose, and stachyose). The excluded volume model for the effect of a single saccharide on the stability of a protein previously proposed by Beg et al. [Biochemistry 54 (2015) 3594] was extended to treat the case of saccharide mixtures. The extended model predicts quantitatively the stabilizing effect of all monosaccharide mixtures on α–LA and lysozyme reported here, as well as previously published results obtained for ribonuclease A [Biophys. Chem. 138 (2008) 120] to within experimental uncertainty.

Conversion of Sugar Cane Molasses to 5-Hydroxymethylfurfural Using Molasses and Bagasse-Derived Catalysts

5-hydroxymethylfurfural (HMF) is a suitable platform chemical for the production of useful chemicals and fuels. This study examined the hydrolysis of mixtures of sucrose, glucose and fructose (synthetic sugar cane molasses) and industrial molasses using sulfonated carbon-based heterogeneous catalysts derived from sugar cane bagasse (B-SO3H) and sugar cane molasses (M-SO3H). Microwave heating at 150 °C for 4 h produced 39 mol % HMF yield with B-SO3H on the synthetic sugar solution. The addition of a molasses pretreatment step, as well as, increasing the microwave power was necessary to achieve similar HMF yields with the industrial molasses sample. The hydrolysis of molasses pretreated with formic acid or HCl resulted in a slightly higher HMF yield than the hydrolysis of molasses pretreated with H2SO4. The buffering capacity of the formic acid pretreated molasses was lowered through dilution with water; and subsequently HMF yield was increased to 64.3 mol % (170 °C, 3 h).

Carbohydrate solutions and contribute to the improvement of physical performance during a high-intensity and long-lasting physical exercise: evaluation of a health claim pursuant to Article 13(5) of Regulation (EC) No1924/2006.

Following an application from Specialised Nutrition Europe (SNE), submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to carbohydrate solutions and contribute to the improvement of physical performance during a high‐intensity and long‐lasting physical exercise. The scope of the application was proposed to fall under a health claim based on newly developed scientific evidence. The food proposed by the applicant as the subject of the health claim is carbohydrate solutions containing glucose, mixtures of glucose and fructose, sucrose and/or maltodextrins. The Panel considers that carbohydrate solutions are sufficiently characterised in relation to the claimed effect. The claimed effect proposed by the applicant is ‘contribute to the improvement of physical performance during a high‐intensity and long‐lasting physical exercise’, which is considered by the Panel as a beneficial physiological effect. The Panel concludes that a cause and effect relationship has been established between the consumption of carbohydrate solutions and the improvement of physical performance during high‐intensity and long‐lasting physical exercise. The target population is healthy trained adults performing high‐intensity (at least at 65% of the VO 2max) and long‐lasting (at least 60 min) physical exercise.

Distinguishing Biologically Relevant Hexoses by Water Adduction to the Lithium-Cationized Molecule.

A method to distinguish the four most common biologically relevant underivatized hexoses, d-glucose, d-galactose, d-mannose, and d-fructose, using only mass spectrometry with no prior separation/derivatization step has been developed. Electrospray of a solution containing hexose and a lithium salt generates [Hexose+Li]+. The lithium-cationized hexoses adduct water in a quadrupole ion trap. The rate of this water adduction reaction can be used to distinguish the four hexoses. Additionally, for each hexose, multiple lithiation sites are possible, allowing for multiple structures of [Hexose+Li]+. Electrospray produces at least one structure that reacts with water and at least one that does not. The ratio of unreactive lithium-cationized hexose to total lithium-cationized hexose is unique for the four hexoses studied, providing a second method for distinguishing the isomers. Use of the water adduction reaction rate or the unreactive ratio provides two separate methods for confidently (p ≤ 0.02) distinguishing the most common biologically relevant hexoses using only femtomoles of hexose. Additionally, binary mixtures of glucose and fructose were studied. A calibration curve was created by measuring the reaction rate of various samples with different ratios of fructose and glucose. The calibration curve was used to accurately measure the percentage of fructose in three samples of high fructose corn syrup (<4% error).

Improving Fructose Utilization and Butanol Production by Clostridium acetobutylicum via Extracellular Redox Potential Regulation and Intracellular Metabolite Analysis.

Jerusalem artichoke (JA) can grow well in marginal lands with high biomass yield, and thus is a potential energy crop for biorefinery. The major biomass of JA is from tubers, which contain inulin that can be easily hydrolyzed into a mixture of fructose and glucose, but fructose utilization for producing butanol as an advanced biofuel is poor compared to glucose-based ABE fermentation by Clostridium acetobutylicum. In this article, the impact of extracellular redox potential (ORP) on the process is studied using a mixture of fructose and glucose to simulate the hydrolysate of JA tubers. When the extracellular ORP is controlled above -460 mV, 13.2 g L-1 butanol is produced from 51.0 g L-1 total sugars (40.1 g L-1 fructose and 10.9 g L-1 glucose), leading to dramatically increased butanol yield and butanol/ABE ratio of 0.26 g g-1 and 0.67, respectively. Intracellular metabolite and q-PCR analysis further indicate that intracellular ATP and NADH availabilities are significantly improved together with the fructose-specific PTS expression at the lag phase, which consequently facilitate fructose transport, metabolic shift toward solventogenesis and carbon flux redistribution for butanol biosynthesis. Therefore, the extracellular ORP control can be an effective strategy to improve butanol production from fructose-based feedstock.

Analysis of the germination proteins in Alicyclobacillus acidoterrestris spores subjected to external factors.

The presence of Alicyclobacillus acidoterrestris, a thermoacidophilic and spore-forming bacterium, in pasteurized acidic juices poses a serious problem for the processing industry. Therefore, the use of other more effective techniques, such as high hydrostatic pressure (HHP) and supercritical carbon dioxide (SCCD), is considered for preserving juices in order to inactivate these bacteria, while reducing the loss of nutrients and sensory quality of juices. On the other hand, HHP and SCCD when combined with a moderately elevated temperature can induce germination of bacterial spores, making them more vulnerable to inactivation. The spore germination can be also induced by nutrients, such as L-alanine or a mixture of asparagine, glucose, fructose and potassium ions (AGFK). The aim of this work was to determine whether applying activating agents: HHP, SCCD and nutrient germinants (L-alanine and the AGFK mixture), could influence the number of spores which start to germinate and how this affects the proteins involved in the spore germination. SDS-PAGE was used to resolve proteins isolated from the A. acidoterrestris spores. The results that were obtained indicate that the germination of A. acidoterrestris spores treated with HHP, SCCD and nutrient germinants reflect the number of spores which start to germinate. The SDS-PAGE data indicated changes in the level of selected proteins occurring when subjected to the germination activating factors as well as noticeable differences in those proteins' molecular weights.

Biosynthesis of P(3HB-co-3HHx) with improved molecular weights from a mixture of palm olein and fructose by Cupriavidus necator Re2058/pCB113

A combination of palm olein (POl) and fructose was used as carbon source for the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] by recombinant Cupriavidus necator Re2058/pCB113. Cultures grown using 5g/L PO alone as carbon source produced cell dry weight (CDW) of 5.13g/L, 67% PHA/CDW and accumulated a copolymer containing 27mol% 3HHx in shake flask cultures. When cultures were grown in 5g/L fructose alone as the carbon source they produced CDW of 2.32g/L, 11% PHA/CDW and accumulated only poly(3-hydroxybutyrate) [P(3HB)] homopolymer. When the cells were cultured in 5g/L POl in combination with 7g/L fructose, CDW of 7.41g/L and 80% PHA/CDW was obtained with 17mol% 3HHx monomer fraction. Biosynthesis was carried out using a 13L fermenter to study the accumulation of 3HHx monomer fraction in the bacterial cells at different time point. The molecular weights of P(3HB-co-3HHx) with 4–15mol% 3HHx monomer were in the range between 5.47–6.85×105Da, which were at least two fold higher than previously reported values. Interestingly, the increase in Mw of the copolymer along with the increase in 3HHx molar fraction was observed. The viscoelastic property of the copolymer further confirmed the increase in Mw.