Formation Of Mixed Micelles Research Articles

A novel cationic Gemini surfactant with amide group: synthesis and micellization behavior in aqueous solutions

Novel cationic Gemini surfactant with amide group, N 1,N 10-bis(3-dodecanamidopropyl)-N 1,N 1,N 10,N 10-tetramethyldecan-1,10-diammonium bromide (abbreviated as 2C12enAm) has been synthesized by two-step reactions, purified and characterized in our laboratory. The critical micelle concentration (CMC) values of this Gemini surfactant were determined using electrical conductivity and surface tension measurements in the range of temperatures from 298.15 to 328.15 K. It was observed that the CMC value of Gemini surfactant has a much lower value compared with monomeric ones and shows a slight increase with an increase in the temperature. The interactions between Gemini and conventional monomeric surfactant hexadecyltrimethylammonium bromide (CTAB) have been studied using the conductivity at a certain micellar concentration and temperature range. Rubingh’s theory is used to evaluate the micellar interaction parameters (β) for the mixed systems. It has been observed that mixed micelle formation in all the binary mixtures exhibited negative synergism (antagonism) or ideal behavior due to positive β values. Thermodynamics parameters were also obtained from the temperature dependence of the CMC values. From the surface tension measurements, CMC, maximum surface excess concentration at the solution/air interface (Γmax), minimum area per surfactant molecule (A min), the surface pressure at CMC (ΠCMC), the surfactant concentration required to reduce the surface tension of solvent by 20 mN m−1 (pC20), and thermodynamic parameters have been evaluated at same temperatures.

Critical micelle concentration values for different surfactants measured with solid-phase microextraction fibers.

The amphiphilic nature of surfactants drives the formation of micelles at the critical micelle concentration (CMC). Solid-phase microextraction (SPME) fibers were used in the present study to measure CMC values of 12 nonionic, anionic, cationic, and zwitterionic surfactants. The SPME-derived CMC values were compared to values determined using a traditional surface tension method. At the CMC of a surfactant, a break in the relationship between the concentration in SPME fibers and the concentration in water is observed. The CMC values determined with SPME fibers deviated by less than a factor of 3 from values determined with a surface tension method for 7 out of 12 compounds. In addition, the fiber-water sorption isotherms gave information about the sorption mechanism to polyacrylate-coated SPME fibers. A limitation of the SPME method is that CMCs for very hydrophobic cationic surfactants cannot be determined when the cation exchange capacity of the SPME fibers is lower than the CMC value. The advantage of the SPME method over other methods is that CMC values of individual compounds in a mixture can be determined with this method. However, CMC values may be affected by the presence of compounds with other chain lengths in the mixture because of possible mixed micelle formation. Environ Toxicol Chem 2016;35:2173-2181. © 2016 SETAC.

The effect of alkyl chain length on synergistic effects in micellization and surface tension reduction in nonionic gemini (S-10) and anionic surfactants mixtures

Mixtures of nonionic gemini surfactant S-10 (α, α'-[2,4,7,9-tetrametyl-5-decyne-4,7-diyl]bis[Ͽ-hydroxy-polioxyetylene]) with anionic SRS (sodium alkylsulfates) and SRSO3 (sodium alkylsulfonates) of different alkyl chain length (from 10 to 14 carbon atoms) were investigated. Surface tension measurements were used to find the cmc values and other adsorption parameters. The Clint, Rubingh⿿s and Rosen theories were applied for evaluation of the synergistic effects in mixed films and mixed micelles formation. It has been documented that in the process of micellization strong synergy exists, while in mixed film formation it is not so evident. In mixtures of S-10 with monomeric surfactants, the synergistic interactions in mixed micelles formation increase with the chain length of monomeric surfactant. The composition of mixed films is different than the composition of mixed micelles, because molar fraction of S-10 in monolayers is much higher than its contribution in mixed micelles at the same bulk composition. The size of micelles is the biggest for S-10 and in case of surfactants mixtures is smaller than that, for individual components.

Poloxamer 407/188 binary thermosensitive hydrogels as delivery systems for infiltrative local anesthesia: Physico-chemical characterization and pharmacological evaluation

In this study, we reported the development and the physico-chemical characterization of poloxamer 407 (PL407) and poloxamer 188 (PL188) binary systems as hydrogels for delivering ropivacaine (RVC), as drug model, and investigate their use in infiltrative local anesthesia for applications on the treatment of post-operative pain. We studied drug-micelle interaction and micellization process by light scattering and differential scanning calorimetry (DSC), the sol-gel transition and hydrogel supramolecular structure by small-angle-X-ray scattering (SAXS) and morphological evaluation by Scanning Electron Microscopy (SEM). In addition, we have presented the investigation of drug release mechanisms, in vitro/in vivo toxic and analgesic effects. Micellar dimensions evaluation showed the formation of PL407-PL188 mixed micelles and the drug incorporation, as well as the DSC studies showed increased enthalpy values for micelles formation after addition of PL 188 and RVC, indicating changes on self-assembly and the mixed micelles formation evoked by drug incorporation. SAXS studies revealed that the phase organization in hexagonal structure was not affected by RVC insertion into the hydrogels, maintaining their supramolecular structure. SEM analysis showed similar patterns after RVC addition. The RVC release followed the Higuchi model, modulated by the PL final concentration and the insertion of PL 188 into the system. Furthermore, the association PL407-PL188 induced lower in vitro cytotoxic effects, increased the duration of analgesia, in a single-dose model study, without evoking in vivo inflammation signs after local injection.

Effect of 1-ethyl-3-methylimidazolium bromide on interfacial and aggregation behavior of mixed cationic and anionic surfactants

Aggregation behavior and interaction of sodium dodecyl sulfate (SDS), tetradecyl trimethyl ammonium bromide (TTAB) and their mixtures with an excess of anionic surfactant in the presence of imidazolium-based ionic liquid, 1-ethyl-3-methylimidazolium bromide (EMIm Br) were investigated using surface tension, electrical conductivity, dynamic light scattering (DLS), transmission electron microscopy (TEM) and cyclic voltammetry measurements. Different physicochemical properties such as critical micelle concentration (CMC), degree of counterion dissociation (αdiss), interfacial properties, the standard Gibbs energy of adsorption and the size of aggregates were determined. The effects of ionic liquid (IL) as additive on the interactions between the surfactants; TTAB, SDS and their mixtures were analyzed on the basis of regular solution theory, both for mixed monolayers at the air/liquid interface (βδ) and for mixed micelles (βM). Interaction parameter values suggested that the formation of aggregates is due to the synergistic interactions in the case of SDS/TTAB systems and becomes more affected by adding IL. It was shown that an excess of cationic surfactant and IL resulted in an increase in the nano-aggregate sizes. The synergism observed for three component systems (surfactant–water–IL) revealed the effect of attractive electrostatic interaction, formation of a three-dimensional hydrogen bond network and hydrophobic forces on mixed micelle formation.

Conductometric study of the mixing behaviour of sodium benzoate and salicylate with an anionic surfactant sodium lauroylsarcosinate

ABSTRACTEffects of two anionic hydrotropes – sodium benzoate (NaBz) and sodium salicylate (NaSal) – on the mixed-micelle formation with an amino-acid-based surfactant – sodium lauroylsarcosinate (SLS) – in water were investigated by the conductometric method. Specific conductivity was measured for SLS/NaBz/water and SLS/NaSal/water systems to determine the critical micelle concentration (cmc). Using the regular solution theory for non-ideal mixing, the pairwise interaction parameter, β12, and micellar composition, χ, were estimated in the mixed micelle. The cmc values of the surfactant–hydrotropes mixtures were generally lower than those predicted from the ideal mixing theory. The β12 values are generally negative for the two systems at all mole fractions with an average value of −2.83 for the SLS/NaBz and −3.31 for SLS/NaSal systems, respectively, indicative of a strong attractive interaction between the SLS/NaBz and SLS/NaSal mixed micelle. The calculated thermodynamic parameters of micellisation all indicated spontaneity in mixed-micelle formation for the systems studied.

Ex-vivo absorption study of a nanoparticle based novel drug delivery system of vitamin D3 (Arachitol Nano™) using everted intestinal sac technique

Cholecalciferol, is a fat soluble vitamin D3 (Vit.D3), recognized in promoting bone health in children and adults and also it has other health benefits. Vit.D3 has suggested being dependent on bile salts for the formation of mixed micelles which is a pre requisite for its absorption. The efficiency of oral absorption of conventional Vit.D3 is approximately 50 %. Arachitol Nano™ is a commercially available nanoparticle based novel drug delivery system (NDDS) of Vit.D3. Data on the uptake of Vit.D3 from the NDDS (Arachitol Nano™) through the intestinal epithelium is not documented. The aim of the study was to investigate the absorption of a nanoparticle based NDDS of Vit.D3 (Arachitol Nano™) using everted rat intestinal sac technique. The results of the study suggested that Vit.D3 from Arachitol Nano™ showed a nonspecific absorption through various segments of rat small intestine, with high flux, permeability coefficient and percentage of absorption (79.21 ± 0.23, 76.55 ± 0.24 and 77.73 ± 0.24 % for duodenum, jejunum and ileum). The average absorption of Arachitol Nano™ was 77.83 ± 0.24 % through rat small intestine. The predicted human absorption may be more than 90 %. The study demonstrates that water soluble nanoparticle Vit.D3 (Arachitol Nano™) can substantially increase the oral absorption of the vitamin D3 and thus can be useful in the oral delivery of vitamin D3.

Micellization behavior of binary mixtures of amino sulfonate amphoteric surfactant with different octylphenol polyoxyethylene ethers in aqueous salt solution: Both cationic and hydrophilic effects

The effect of different valent inorganic cations and the hydrophilic group of surfactant on the micellization behavior of binary surfactant mixtures constituted by an amphoteric surfactant, sodium 3-(N-dodecyl ethylenediamino)-2-hydropropyl sulfonate (C12AS), and three nonionic surfactants octylphenol polyoxyethylene ether (OP-n) with different numbers of oxyethylene glycol ethers (n), namely, OP-10, OP-7, and OP-4 was investigated in aqueous solution. These inorganic cations include Na+, K+, Mg2+, Ca2+, Ba2+, Al3+ and Fe3+. Both the tensiometry and the UV–vis spectrophotometry using pyrene as a probe were adopted to determine the critical micelle concentrations (cmc) of individual or mixed surfactants. Interaction parameters between two surfactants and other parameters were obtained based on the regular solution theory, the pseudophase separation model, Rubingh's model, etc. Thermodynamic parameters including Gibbs energy of micellization and thermodynamic stability were also calculated by both the equation proposed by Molyneux et al. and Maeda's treatment, respectively. The effect of different valent cations on both the mixed cmc of surfactant mixtures and the molar fraction of C12AS in mixed micelle (X1) can be explained theoretically by the salting-out effect, electrostatic interaction and steric effect. For three binary surfactant mixtures, the chain length of hydrophilic group of surfactant results in different micellization behaviors. On adding salts, the interaction parameters show that there exists a synergistic effect between two surfactants, and the effect increases with increasing the ratio of valence (Z) and atom radius (R) of cation, especially, for the C12AS/OP-4 mixture. With increasing the Z/R value of cation, a deviation of X1 from the ideal value shows a divergence for three binary surfactant mixtures because of different hydrophilicities of surfactant. Thermodynamic parameters indicate that the addition of cations with a large Z/R value can be contributive more effectively to the formation of stable mixed micelle than the case in the presence of cations with a small Z/R value.

Multidetector Thermal Field-Flow Fractionation: A Unique Tool for Monitoring the Structure and Dynamics of Block Copolymer Micelles

Block copolymer micelles have attracted much attention as a versatile platform that can readily be adapted to a wide range of applications including drug delivery and the production of nanoscale patterns. However, current analytical techniques are not suitable to provide comprehensive information regarding size, molar mass, chemical composition, and micelle stability in different environments. It is shown by the analysis of polybutadiene–polystyrene micelles with various corona compositions that, in contrast to current techniques, multidetector thermal field-flow fractionation (ThFFF) is capable of separating micelles according to corona composition and providing comprehensive information on important micelle characteristics such as size, molar mass, chemical composition, and their respective distributions from a single analysis. Moreover, it is shown that ThFFF is a suitable technique to monitor the dynamics of mixed micelle formation in terms of size, molar mass, and chemical composition.

Perfectly Wetting Mixtures of Surfactants from Renewable Resources: The Interaction and Synergistic Effects on Adsorption and Micellization.

This paper presents a study of the surface properties of mixtures of surfactants originating from renewable sources, i.e., alkylpolyglucoside (APG), ethoxylated fatty alcohol (AE), and sodium soap (Na soap). The main objective was to optimize the surfactant ratio which produces the highest wetting properties during the analysis of the solution of the individual surfactants, two- and three-component mixtures, and at different pH values. The results showed the existence of a synergistic effect in lowering the interfacial tension, critical micelle concentration and the formation of mixed micelles in selected solutions. We found that best wetting properties were measured for the binary AE:APG mixtures. It has been demonstrated that slightly lower contact angles values were observed on Teflon and glass surfaces for the AE:APG:soap mixtures but the results were obtained for higher concentration of the components. In addition, all studied solutions have very good surface properties in acidic, basic and neural media. However, the AE:soap (molar ratio of 1:2), AE:APG (2:1) and AE:APG:soap (1:1:1) compositions improved their wetting power at pH 7 on the aluminium and glass surfaces, as compared to solutions at other pH values tested (selected Θ values close to zero—perfectly wetting liquids). All described effects detected would allow less surfactant to be used to achieve the maximum capacity of washing, wetting or solubilizing while minimizing costs and demonstrating environmental care.

The formation of mixed micelles of sugar surfactants and phospholipids and their interactions with hyaluronan

The aggregation of sugar surfactants and the incorporation of phospholipids into sugar surfactant micelles were investigated by means of fluorescence spectroscopy. Two representatives from the family of alkyl glucosides were studied: dodecyl-β-D-maltoside and octyl-β-D-glucopyranoside. The presence of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) in the sugar surfactant system promoted the formation of new structures at sugar surfactant concentration below its critical micelle concentrations. According to the aggregation number measurements studied by a fluorescence quenching of hexadecylpyridinium chloride measurements, the premicellar aggregates formed from octyl-β-D-glucopyranoside and DPPC were composed of the third of molecules compared to the octyl-β-D-glucopyranoside micelles. Interactions of the formed mixed micelle systems composed of sugar surfactant and DPPC with hyaluronan were explored. The addition of hyaluronan had different effects on the dodecyl-β-D-maltoside/DPPC and octyl-β-D-glucopyranoside/DPPC mixed systems. In addition, the mixed system of dodecyl-β-D-maltoside and lecithin was studied, but possibly, only a coexistence of lipid aggregates and sugar surfactant micelles was observed.

Synergistic solubilization of polycyclic aromatic hydrocarbons by mixed micelles composed of a photoresponsive surfactant and a conventional non-ionic surfactant

Abstract Surfactant-enhanced remediation is a powerful technology for soil clean-up. However, some drawbacks, such as its high cost and the secondary contamination caused by flushing agents, have hampered this technology and its commercial application has been largely limited to North American and European countries. The aim of this work is to introduce a novel reversible solubilization system composed of the photoresponsive surfactant 4-butylazobenzene-4′-(oxyethyl)trimethylammonium bromide (AZTMA) and the commercial non-ionic surfactant Tween80 for the solubilization of organic contaminants. The solubilization behavior of the AZTMA/Tween80 surfactant system toward polycyclic aromatic hydrocarbons (PAHs) has been studied. Critical micelle concentration (CMC) values indicate that the mixed system has a synergism in mixed micelle formation, and that the interaction parameter ( β ) is −2.85 when the mole ratio is 2:8. AZTMA is also predicted to be low biologic toxicity by the CMC and surface tension. The synergistic effect on molar solubilization ratio (MSR) and micelle-phase/aqueous-phase partition coefficient K mc values is related to the composition and concentration of the mixed system and follows the order pyrene > phenanthrene > acenaphthene, and these parameters are much higher than those for the single surfactants. After irradiation with UV light, more than 65% of the PAHs could be separated from the mixed micelles, and the cumulative release efficiencies of selected PAHs are consistent with the results of solubilization experiments. All of these features demonstrate that mixed AZTMA/Tween80 should be preferable to single surfactants for soil remediation, avoiding the issues associated with chemical flushing agents and decreasing remediation costs.

Bioaccessibility study of plant sterol-enriched fermented milks.

The bioaccessibility (BA) of total and individual plant sterols (PS) of four commercial PS-enriched fermented milk beverages (designated as A to D) was evaluated using in vitro gastrointestinal digestion including the formation of mixed micelles. The fat content of the samples ranged from 1.1 to 2.2% (w/w), and PS enrichment was between 1.5 and 2.9% (w/w). β-Sitosterol, contained in all samples, was higher in samples A and B (around 80% of total PS). The campesterol content was C (22%) > A (7%) > B (5%). Sitostanol was the most abundant in sample D (85%). Stigmasterol was only present in sample C (33%). The greatest BA percentage for total PS corresponded to samples A and B (16-17%), followed by sample D (11%) and sample C (9%). The total BA was not related to the protein, lipid or PS content of the beverages, whereas samples with higher carbohydrates and fiber contents showed lower BA. The BA of the individual PS differed according to the sample considered, and was not related to the PS profile of the sample, thus indicating strong dependency upon the matrix (PS ingredient and other components). Although in vivo studies should be carried out to better assess the functionality of PS in functional foods such as enriched fermented milk beverages, our in vitro study is a useful preliminary contribution to evaluation of the efficacy of these products.

Thermodynamics of micelle formation in a water–alcohol solution of sodium tetradecyl sulfate

The effects of addition of ethanol and propan-1-ol on sodium tetradecyl sulfate micelle formation in an aqueous solution are studied via microprobe fluorescence microscopy and conductometry. The critical micelle concentration, quantitative characteristics of micelles, and thermodynamic parameters of micelle formation are determined. Addition of 5–15 vol % of ethanol or 5–10 vol % of propan-1-ol is shown to result in a lower critical micelle concentration than in the aqueous solution, and in the formation of mixed spherical micelles whose sizes and aggregation numbers are less than those for the systems without alcohol. The contribution from the enthalpy factor to the free energy of sodium tetradecyl sulfate micelle formation is found to dominate in mixed solvents, in contrast to aqueous solutions.

Mixed Micellization Between an Antidepressant Drug Imipramine Hydrochloride and Surfactants (Conventional/Gemini) at Different Temperatures and Compositions

The micellization behavior of an amphiphilic antidepressant drug imipramine hydrochloride (IMP) in the presence of a conventional (CTAB) and three gemini (16-s-16, s = 4–6) cationic surfactants has been studied at different temperatures and compositions by conductometry. The experimental and ideal critical micelle concentrations, cmc and cmc id, values indicate mixed micelle formation between the components. The micellar mole fractions of surfactants ( $$ X_{1}^{\text{Rub}} $$ , $$ X_{1}^{\text{M}} $$ , $$ X_{1}^{\text{Rod}} $$ and $$ X_{1}^{\text{id}} $$ ), calculated using different theoretical models, show greater contribution from the surfactants. The interaction parameter (β) is negative at all temperatures and at all compositions indicating attractive interactions. Activity coefficients (f 1 and f 2) are always less than unity reflecting nonideality in the systems. Thermodynamic parameters which were also evaluated suggest dehydration of the hydrophobic part of the drug at higher temperatures.

Synergistic effects in micellization and surface tension reduction in nonionic gemini S-10 and cationic RTAB surfactants mixtures

Abstract Mixtures of nonionic gemini surfactant S-10 (α,α′-[2,4,7,9-tetrametyl-5-decyne-4,7-diyl]bis[ω-hydroxy -polioxyetylene] with cationic alkyltrimethylammonium bromides of different alkyl chain length (RTAB) were investigated. Basing on surface tension measurements the cmc values and other adsorption parameters were found. The Clint, Rubing’s and Rosen theories were applied for evaluation of the synergistic effects in mixed films and mixed micelles formation. The molecular interaction parameters have negative values in all investigated mixtures, which confirm attractive forces between components. The magnitude of this forces depends on mixtures composition and in mixed micelles was higher than that in mixed films. It has been documented that in the process of micellization strong synergy exists, while in mixed film formation it is not so evident and was stated only in surface tension reduction efficiency. The composition of mixed films is different than the composition of mixed micelles, because a molar fraction of S-10 in monolayers is much higher than its contribution in mixed micelles at the same bulk composition.

Morphology study of DMPC/DHPC mixtures by solution-state 1H, 31P NMR, and NOE measurements

The temperature and mixing-ratio dependencies are studied by solution-state NMR for the morphology of aggregates composed of dimyristoylphosphatidylcholine (DMPC) and dihexanoylphosphatidylcholine (DHPC). The 1H and 31P NMR and nuclear Overhauser effect (NOE) measurements were performed at 25–60°C for molar ratio of q=1–5 (q=[DMPC]/[DHPC]). For the q=1 and 2 systems, the turbidity and viscosity of the sample are unchanged and the 1H and 31P NMR spectra are sharp over the whole temperature range examined. Combined with the NOE measurement and compared to the results for small unilamellar vesicle system, the q=1 and 2 aggregates are found to form the spherical mixed micelle and/or small bicelle over 25–60°C. When q is larger and is 2.5 or 3, the lipid-mixture system exhibits two morphological changes with temperature variation. At low temperature, the DMPC/DHPC system is of spherical mixed micelle and/or small bicelle, as evidenced from the visual observation, the 1H and 31P NMR spectra, and the NOE measurement. In the intermediate temperature range, the aggregate is the magnetically oriented bicelle since the solution is observed to be opaque and viscous with the 1H signal broadening and the 31P signal splitting into two peaks. When the temperature is high and above ~50°C, the sample becomes milky with sharp 1H and singlet 31P signals. The NOE measurement shows that the lipid dynamics of the aggregate is faster than of the large unilamellar vesicle with low curvature. These show the formation of cylindrical mixed micelle.

Supersaturation-Limited and Unlimited Phase Spaces Compete to Produce Maximal Amyloid Fibrillation near the Critical Micelle Concentration of Sodium Dodecyl Sulfate.

Although various natural and synthetic compounds have been shown to accelerate or inhibit the formation of amyloid fibrils, the mechanisms by which they achieve these adverse effects in a concentration-dependent manner currently remain unclear. Sodium dodecyl sulfate (SDS), one of the compounds that has adverse effects on fibrillation, is the most intensively studied. Here we examined the effects of a series of detergents including SDS on the amyloid fibrillation of β2-microglobulin at pH 7.0, a protein responsible for dialysis-related amyloidosis. In all the detergents examined (i.e., SDS, sodium decyl sulfate, sodium octyl sulfate, and sodium deoxycholate), amyloid fibrillation was accelerated and inhibited at concentrations near the critical micelle concentration (CMC) and higher than CMC, respectively. The most stable conformation changed from monomers with a β-structure to amyloid fibrils with a β-structure and then to α-helical complexes with micelles with an increase in detergent concentrations. These results suggest that competition between supersaturation-limited fibrillation and unlimited mixed micelle formation between proteins and micelles underlies the detergent concentration-dependent complexity of amyloid fibrillation.

Gelation and micellization behaviors of pluronic® F127 hydrogel containing poly(isobutylcyanoacrylate) nanoparticles specifically designed for mucosal application

The aim of this investigation is to combine the advantages of pluronic® F127 hydrogels and nanoparticles composed of poly(isobutylcyanoacrylate) (PIBCA) core coated with a mixture of chitosan and thiolated chitosan to design novel multifunctional formulation for mucosal application. Nanoparticles offer the advantage of being mucoadhesive while pluronic® F127 hydrogel allowed prolonged contact time onto mucosal surfaces. This work highlights an unprecedented comprehensive study on the effect of nanoparticles on gelation and micellization behaviors of pluronic® F127 using rheology and micro-calorimetry experiments. Results showed that presence of nanoparticles induced (i) smaller crystal peak of F127, (ii) a decrease of the enthalpy of F127 micellization and (iii) a non-reversibility of micelle formation (during heating ramp) and micelle melting (during cooling ramp). Together, these findings suggest that a part of F127 was not able to associate into micelles and the formation of mixed micelles containing F127 unimers and PIBCA/(chitosan/thiolated chitosan) copolymer and/or PIBCA homopolymer was suspected. The interaction of F127 unimers with nanoparticles resulted from their physical de-structuration as revealed by nanoparticle size measurement. In addition, we found that short polymerization duration of one hour induced more pronounced nanoparticle de-structuration. Twenty-four hour-polymerization of isobutylcyanoacrylate in the presence of chitosan and thiolated chitosan led to more stable nanoparticles when mixed with pluronic® F127.

Aggregation behavior and intermolecular interaction of binary surfactant mixtures based on cationic Geminis and nonionic surfactants

The Gemini surfactants of type alkylediyl-α-ω-bis(alkyldimethylammonium) dibromide with different alkyl groups containing m carbon atoms and an ethanediyl spacer, referred as “m-2-m” [CmH2m + 1(CH2)2 N+(CH2)2 N+(CH2)2 CmH2m + 1, 2Br−] (m = 12 and 16), have been synthesized, purified, and characterized in our laboratory. The interactions between cationic Geminis and nonionic surfactants polyoxyethylene (23) lauryl ether (Brij35) and polyoxyethyleneoctyl phenyl ether (TX-100) have been studied by conductivity and steady-state fluorescence quenching techniques at a certain micellar concentration range and certain temperature. Rubingh’s theory is used to evaluate the micellar interaction parameters (β) for the mixed systems. It has been observed that mixed micelle formation in all the binary mixtures exhibited negative synergism (antagonism) or ideal behavior due to positive β values. It was observed that contribution of conventional surfactants was always more than that of the Geminis. By using the ratio of peak intensities, micro-polarity, dielectric constant (D), and binding constant (Ksv) of mixed systems have been found.