The formation of mixed micelles of sugar surfactants and phospholipids and their interactions with hyaluronan

Abstract

The aggregation of sugar surfactants and the incorporation of phospholipids into sugar surfactant micelles were investigated by means of fluorescence spectroscopy. Two representatives from the family of alkyl glucosides were studied: dodecyl-β-D-maltoside and octyl-β-D-glucopyranoside. The presence of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) in the sugar surfactant system promoted the formation of new structures at sugar surfactant concentration below its critical micelle concentrations. According to the aggregation number measurements studied by a fluorescence quenching of hexadecylpyridinium chloride measurements, the premicellar aggregates formed from octyl-β-D-glucopyranoside and DPPC were composed of the third of molecules compared to the octyl-β-D-glucopyranoside micelles. Interactions of the formed mixed micelle systems composed of sugar surfactant and DPPC with hyaluronan were explored. The addition of hyaluronan had different effects on the dodecyl-β-D-maltoside/DPPC and octyl-β-D-glucopyranoside/DPPC mixed systems. In addition, the mixed system of dodecyl-β-D-maltoside and lecithin was studied, but possibly, only a coexistence of lipid aggregates and sugar surfactant micelles was observed.