Mixed Fruit Juice Research Articles

Hyperactivity in Children Linked to Food Coloring

Hyperactivity in Children Linked to Food Coloring

Four week supplementation with mixed fruit and vegetable juice concentrates increased protective serum antioxidants and folate and decreased plasma homocysteine in Japanese subjects.

Fruit and vegetable consumption has been inversely associated with the risk of chronic diseases including cancer and cardiovascular disease, with the beneficial effects attributed to a variety of protective antioxidants, carotenoids and phytonutrients. The objective of the present study was to determine the effect of supplementation with dehydrated concentrates from mixed fruit and vegetable juices (Juice Plus+R) on serum antioxidant and folate status, plasma homocysteine levels and markers for oxidative stress and DNA damage. Japanese subjects (n=60; age 27.8 yrs; BMI 22.1) were recruited to participate in a double-blind placebo controlled study and were randomized into 2 groups of 30, matched for sex, age, BMI and smoking status (39 males, 22 smokers; 21 females, 13 smokers). Subjects were given encapsulated supplements containing mixed fruit and vegetable juice concentrates or a matching placebo for 28 days, with blood and urine samples collected at baseline, day 14 and day 28 for analytical testing. Compared with the placebo, 28 day supplementation significantly increased the concentration of serum beta-carotene 528% (p<0.0001), lycopene 80.2% (p<0.0005), and alpha tocopherol 39.5% (p<0.0001). Serum folate increased 174.3% (p<0.0001) and correlated with a decrease in plasma homocysteine of -19.9% (p<0.03). Compared with baseline, measures of oxidative stress decreased with serum lipid peroxides declining -10.5% (p<0.02) and urine 8OHdG decreasing -21.1% (p<0.02). Evaluation of data from smokers only (n=17) after 28 days of active supplementation showed comparable changes. In the absence of dietary modification, supplementation with the fruit and vegetable juice concentrate capsules proved to be a highly bioavailable source of phytonutrients. Important antioxidants were elevated to desirable levels associated with decreased risk of disease while markers of oxidative stress were reduced, and folate status improved with a concomitant decrease in homocysteine, and these benefits occurred to a similar extent in smokers when compared to non-smokers.

혼합과채주스의 청징 및 품질특성 비교

Clarified mixed fruit and vegetable (apple, carrot and orange) juices were prepared using different clarification methods including centrifugation, ultrafiltration (UF), and combined treatment with the blend ratio of 1:1:2, 1:2:1, and 2:1:1 (apple, orange and carrot). Effects of clarification methods on the clarification efficacy as well as other quality parameters were investigated. Clarification was improved with increase in centrifugation speed but was less affected by lowering temperature. Ultrafiltration process was very effective to produce clarified mixed fruit and vegetable juice. Combined treatment did not significantly improve the clarification efficacy since most clarification was already achieved during ultrafiltration process (p>0.05). L*- and b*-values increased while a*-value decreased significantly after clarification regardless of methods in all blend juices (p

Increased free malondialdehyde concentrations in smokers normalise with a mixed fruit and vegetable juice concentrate: a pilot study

Cigarette smoking, a cardiovascular risk factor leading to oxygen free radical formation, is involved in the development of serious pathological conditions. On the other hand, a healthy diet and adequate supplementation can help prevent many diseases. The aim of our study was to evaluate in healthy light smokers the effects of supplementation with mixed fruit and vegetable juice powder concentrate on homocysteine metabolism and oxidative status. In this pilot study, 32 healthy volunteers, 16 light smokers and 16 non-smokers, on twice daily supplementation were monitored at time zero and after 30 days. Plasma homocysteine, and serum vitamin B(12) and folate concentrations were measured by immunoenzymatic assays; reactive oxygen species, total antioxidant capacity and thiol groups by spectrophotometric methods; and total and free malondialdehyde concentrations by gas chromatography-mass spectrometry with isotopic dilution. Baseline free malondialdehyde concentrations were significantly higher in smokers than in non-smokers and normalised after 30-day supplementation. Baseline results for all the other parameters remained unchanged after supplementation, with no significant differences between smokers and non-smokers. This is the first study showing a significant decrease in free malondialdehyde levels in light smokers after 1-month phytonutrient supplementation.

Supplementation With Mixed Fruit And Vegetable Juice Concentrates Attenuates Oxidative Stress Markers In Trained Athletes

Intensive exercise enhances the formation of free radicals and other reactive oxygen and nitrogen species (RONS) in animals and humans resulting in the generation of compounds like malondialdehyde (MDA) and carbonyl-proteins (CP). Reduced formation of these oxidative stress parameters during physical exercise by supplementation with antioxidants has not been reported. Purpose To investigate the influence of a) supplementation with mixed fruit and vegetable juice concentrates and b) of exercise duration with a defined intensity on the concentrations of MDA and CP after intense mountain biking for two hours. Methods 5 healthy trained men (male; 37 ± 5yrs; VO2max 55 ± 5mL ± kg−1 ± min−1) supplemented daily with 4 capsules of a phytonutrient preparation from fruits and vegetables. Subjects performed a two hours lasting mountain bike exercise to the top of a mountain (altitude 1500m, distance 30km) at 75% of the individual VO2max, followed by a moderate downhill performance (same route) at 45% of the individual VO2max. Capillary blood was collected from the finger tip before the exercise, on the top of the mountain immediately after intense exercise (=two hours) and the third collection at the end of exercise (=three hours). After collection blood plasma samples were separated by centrifugation at 4 ± C and plasma levels of MDA were measured by a HPLC method, measurement of CP was performed after derivatization with dinitrophenylhydrazine (DNPH) by a chemiluminescence technique. A two-sided Student's t-test was performed for testing the significance of differences with p <0.05 as the criterion for significance. Results Both parameters were significantly decreased before exercise (MDA: 2.43 ± 0.39 vs. 1.64 ± 0.52 μM; CP: 0.46 ± 0.05 nmol/mg protein vs. 0.23 ± 0.02 nmol/mg protein), after 2 hours of intense exercise (MDA: 2.68 ± 0.45 vs. 1.85 ± 0.50 μM; CP: 0.55 ± 0.18nmol/mg protein vs. 0.32 ± 0.019nmol/mg protein) and after prolonged exercise of 3 hours (MDA: 2.47 ± 0.73 to 1.64 ± 0.47 μM; CP: 0.5 ± 0.08nmol/mg protein to 0.33 ± 0.09nmol/mg protein) by supplementation compared to the non supplemented phase. No significant difference in the concentrations of both parameters throughout the time course of exercise was estimated except the CP values between the first and second blood collection in the supplemented phase (CP: 0.29 ± 0.02 nmol/mg vs. 0.315 ± 0.02 nmol/mg). Conclusions Thus we demonstrate a benefit of the supplementation with the phytonutrient preparation concerning oxidative protein damage and lipid peroxidation products in human plasma. The chosen exercise duration at a certain intensity had modest influence to the concentrations of oxidative stress markers.

Supplementation with Mixed Fruit and Vegetable Juice Concentrates Increased Serum Antioxidants and Folate in Healthy Adults

Objective: Epidemiological studies have shown that low plasma levels of antioxidant micronutrients, which are commonly found in fruit and vegetables, are associated with increased risk for diseases such as heart disease, cancer, metabolic disorders and the like. The aim of this study was to monitor the dietary habits of a group of healthy, middle-aged, men and women and to assess the effect of supplementation with a natural phytonutrient preparation from fruits and vegetables, on plasma levels of various antioxidant micronutrients and oxidative stress assessed by measuring 8-oxodGuo (8-oxo-7,8-dihydro-2′-deoxyguanosine) in urine.Methods: The study followed a double-blind randomized cross-over design involving 59 healthy men and women (40–60 years of age). The supplement or a placebo was given to two groups for a total period of 14 weeks (crossover week 7). Blood levels of β-carotene, vitamin C, vitamin E, selenium and folate were measured at 0, 7 and 14 weeks. Fruit and vegetable consumption was monitored by means of a retrospective food frequency questionnaire at week 0, 7 and 14. Urinary 8-oxodGuo was also determined at these time points.Results: Significant increases in blood nutrient levels after active supplementation were observed for β-carotene, vitamin C, vitamin E, selenium and folate. Ranges measured, after supplementation, often fell into those associated with a reduced risk for disease. Our data suggests that, although generally health conscious, participants still fell short of the recommended five portions of fruit and vegetables per day. No significant group changes were noted for 8-oxodGuo concentration in urine.Conclusion: Supplementation with mixed fruit and vegetable juice concentrates effectively increased plasma levels of important antioxidant nutrients and folate.

Alicyclobacillus pomorum sp. nov., a novel thermo-acidophilic, endospore-forming bacterium that does not possess omega-alicyclic fatty acids, and emended description of the genus Alicyclobacillus.

A thermo-acidophilic endospore-forming bacterium was isolated from a mixed fruit juice. The organism, strain 3A(T), was rod-shaped, grew aerobically at 30-60 degrees C (optimum 45-50 degrees C), pH 3.0-6.0 (optimum pH 4.0-4.5) and produced acid from various sugars. It contained menaquinone-7 as the major isoprenoid quinone. The G+C content of the DNA was 53.1 mol%. The predominant cellular fatty acids of the strain were iso-C(15 : 0), anteiso-C(15 : 0), iso-C(16 : 0), iso-C(17 : 0) and anteiso-C(17 : 0), but omega-alicyclic fatty acids, which are characteristic of the genus Alicyclobacillus, were not found in the strain. Phylogenetic analyses based on both 16S rRNA and gyrB (DNA gyrase B subunit gene) gene sequences showed that strain 3A(T) falls into the Alicyclobacillus cluster, validated by significant bootstrap values. However, strain 3A(T) did not show a close relationship to the other species of the cluster. The level of 16S rDNA similarity between strain 3A(T) and other strains of the cluster was between 92.5 and 95.5 %. The level of gyrB sequence similarity between strain 3A(T) and other strains of the cluster was between 68.5 and 74.4 %. DNA-DNA hybridization values between strain 3A(T) and phylogenetically related strains of the genera Alicyclobacillus, Bacillus and Sulfobacillus were under 13 %, indicating that strain 3A(T) represents a distinct species. On the basis of these results, strain 3A(T) should be classified as a novel Alicyclobacillus species. The name Alicyclobacillus pomorum is proposed for this organism. The type strain of Alicyclobacillus pomorum is strain 3A(T) (=DSM 14955(T)=IAM 14988(T)).

Nutritional Value and Microbiological Safety of Fresh Fruit Juices sold through Retail Outlets in Qatar

The nutritional value of ten fresh fruit juices purchased from retail outlets in Doha, Qatar was calculated on the basis that approximately 100 g of fruit is used to make one glass of juice (250 ml). Avocado juice was the best source of energy and potassium followed by banana juice, while guava juice was an outstanding source of vitamin C and carotene. By contrast, the microbiological quality of all the products was well outside the Gulf Standards for fruit juices, and coliform counts usually exceeded 1,000 cfu ml . In one sample of mixed fruit juice, the coliform count was above 1.0 x 10 cfu ml , and both 1 6 1

Near Infrared Spectroscopy of Dry Extracts from High Moisture Food Products on Solid Support—A Review

This review article discusses studies published on diffuse near infrared spectroscopy of dry extracts from high moisture foods on solid support. The method can be used for rapid quantification and classification of low concentration nutrient components in aqueous samples. The volume most frequently used for measurement has been 0.5 ± 0.1 mL. The present paper aims to give a full review of journal papers and a selection of other reports, covering the method and applications on mixed model solutions, fruit juices and other beverages and on meat juices.