Mixed Fruit Juice Research Articles

Electrochemical aptasensor for sensitive detection of staphylococcal enterotoxin type A in milk and fruit juice.

Anaptamer-based electrochemical sensor for the sensitive detection of staphylococcal enterotoxin type A(SEA) is presented. The truncated aptamer AptSEA1.4 used in this work was screened using computational techniques, which reduced the cost of the SELEX screening process. The aptamer-SEA interactions were confirmed by employing circular dichroism (CD) and fluorescence spectroscopy. Afterwards, for developing an electrochemical aptasensor, a fabricated GNR/FTO aptasensor was prepared and characterized using scanning electron microscopy-energy-dispersive X-ray analysis (SEM-EDX), atomic force microscopy (AFM), cyclic voltammetry (CV), and square wave voltammetry (SWV). A detailed investigation of aptamer and SEA interaction in the presence of various experimental conditions was also conducted through SWV and electrochemical impedance spectroscopy (EIS). The aptamer exhibits a strong affinity for SEA, with a dissociation constant (Kd) of 19.93nM. The aptasensor is sensitive, with a lower limit of detection of 12.44pgmL-1. It has good stability, repeatability, and specificity and has displayed highly specific and sensitive detection SEA in spiked packaged mixed fruit juice and milk, with a recovery of 95-110%. The aptasensor has highpromise for detecting SEA in other food items.

To assess the effect of selected locally available beverages on salivary pH, flow rate, and oral clearance rate among adults

Aim: The aim of this study is to assess the effect of selected locally available beverages on salivary pH, flow rate, and oral clearance rate among adults. Materials and Methods: A quantitative diagnostic method for dental caries would improve oral health, which directly affects the quality of life. The pH scale measures how acidic or basic a solution is, with 7 being neutral. When the pH of plaque drops below normal, or <5.5, the acidity starts to dissolve minerals and destroy the tooth’s enamel. This clinical trial will be conducted on 40 subjects. Test beverages will be Red Bull, mixed fruit juice, tea, and sweetened milk. Unstimulated salivary samples will be collected for each study subject at least 1 h after their breakfast. After the collection of baseline salivary samples, the subjects will be given one beverage to drink, and then stimulated saliva samples will be collected at fixed time intervals. A digital pH meter will be used to measure the pH at every step. Statistical Analysis: This will be carried out using SPSS version 17, one-way ANOVA, and post hoc Tukey’s test will be applied in the statistical tests. Results: The maximum drop in pH was seen in the case of carbonated beverage (Red Bull), the oral clearance for all the liquid food items was achieved at 14 minutes. Conclusions: Liquids had a considerable cariogenic and erosive potential despite their quick clearance from the mouth cavity. it is always recommended to limit beverage consumption to maintain good dental health.

Impact of common social habits on optical properties of lithium disilicate glass ceramic crowns: An in vitro study

Statement of the problemPatient stratifications considered the stability of color and treatment longevity are key success of restoration. Daily consumption of colored beverages, such as coffee, tea, and soft drinks, as well as the use of globally consumed materials, such as smokeless tobacco (ST), snuff, Khat, and Yerba mate, can change the color of restorative materials, such as lithium disilicate glass ceramics (LDGC). These changes can ultimately lead to treatment failure. PurposeThis in vitro study aimed to evaluate color changes, translucency, and opalescence of full anatomical LDGC crowns exposed to commonly used and potentially colorant solutions. Materials and methodsNinety LDGC specimens/crowns were prepared and divided into nine groups according to immersion solution (control, Saudi Coffee, Cola, Khat, Yerba mate, Nescafe, ST Snuff, and Mixed Fruit Juice). The specimens were immersed in colorant solutions for 15 days with alternating twice daily at 37 °C. Color parameters were measured with a spectrophotometer and calculated using two backgrounds (black and white). Data were subjected to ANOVA followed by the Student t-test and Bonferroni test at a significant difference level (α = 0.05). ResultsThe greatest color change (ΔE*) among groups after immersion was observed in Yerba mate (7.6 ± 1.6). The mean difference of before and after staining within Yerba mate group was 3.14 ± 1.6 (p = 0.001). Translucency mean values of groups after immersion into staining media were ranging between 7.6 ± 1.2 and 9.1 ± 2, showing a slight decrease compared with pre-staining values but was not significantly different. Immersion in Mixed Fruit Juice significantly reduced opalescence (7.4 ± 1.9) compared to (8.8 ± 1.7) before staining. ConclusionThe findings confirm that appropriate user guidance helps to preserve both translucency and opalescence as well as prevent color changes. This can improve patient compliance and promote treatment longevity.

Functional evaluation of pure natural edible Ferment: protective function on ulcerative colitis.

To investigate the therapeutic efficiency of a novel drink termed "Ferment" in cases of ulcerative colitis (UC) and its influence on the gut microbiota. In this study, we developed a complex of mixed fruit juice and lactic acid bacteria referred to as Ferment. Ferment was fed to mice for 35 days, before inducing UC with Dextran Sulfate Sodium Salt. We subsequently investigated the gut microbiome composition using 16S rRNA sequencing. After Ferment treatment, mouse body weight increased, and animals displayed less diarrhea, reduced frequency of bloody stools, and reduced inflammation in the colon. Beneficial bacteria belonging to Ileibacterium, Akkermansia, and Prevotellacea were enriched in the gut after Ferment treatment, while detrimental organisms including Erysipelatoclostridium, Dubosiella, and Alistipes were reduced. These data place Ferment as a promising dietary candidate for enhancing immunity and protecting against UC.

Chemical Profile Characterization of Fruit and Vegetable Juices after Fermentation with Probiotic Strains.

Lactic acid bacteria (LAB) are widely applied for fermentation purposes in dairy and non-dairy food matrices with beneficial technological and health-promoting properties. This study describes the effect of two lactic acid bacteria, namely, Lactiplantibacillus paracasei SP5 and Pediococcus pentosaceus SP2, on the phenolic profiles, antioxidant activities, total phenolic content (TPC), carotenoid content, and sensorial profile of two different mixed fruit juices. After 48 h of fermentation, both LABs retained viability over 9 Log CFU/mL in both juices. The TPC, zeaxanthin + lutein, β-carotene content, and antioxidant activity (AA) were elevated for both LABs and mixed juices after 48 h of fermentation compared to control samples. Regarding the phenolic profile, both juices exhibited a significant decrease in chlorogenic acid levels, while quinic acid and tyrosol concentrations showed notable increases.

A functional and probiotic approach: mixed fruit juice powder with addition of lactobacilli

The aim of this study was to evaluate changes in a mixture of acerola and ciriguela juice (40/60%, respectively) and the juice mixture supplemented with Lactobacillus rhamnosus LPAA 01, L. casei LPAA 02, and L. plantarum LPAA 03, ratio 1:1, both powders obtained by spray drying, during storage. The stability of acerola and ciriguela mixed powder without the addition of microorganisms was evaluated for 90 days at 25°C in different water activities (Aws) (0.11, 0.23, and 0.34). Aw that most effectively maintained the stability of mixed powdered juice was used for the evaluation of the fermented mixed juice powder containing probiotics for 45 days at 5 and 25°C, respectively. Samples were characterized by physicochemical properties, bioactive compounds, and cell viability. The physicochemical properties of the powdered mixed juice without the addition of probiotics remained stable when stored at 25°C at an Aw of 0.11 for 60 days. Ascorbic acid, carotenoids, and total phenolics showed favorable characteristics for the commercialization and bioactive properties of the powders, with potential use as functional ingredients in food. Probiotic powders showed viable cell counts above 6.0 log colony-forming units (CFU)/g after storage for up to 20 days at 5°C and up to 14 days at 25°C.

IJCM_265A: Cellular Consequences of Fruit Juice Metallurgy: A Closer Look at Heavy Metal Influence

Background: The leaching of heavy metals from packaging materials into packed juices can have several adverse effects on the quality and safety of the products and potentially pose health risks to consumers. Objective: The research aimed to investigate the cytotoxic effects of heavy metals specifically vanadium, cadmium, arsenic, and mercury, which are commonly found in packaging materials of marketable fruit juices which are leached into juices itself (orange and mixed fruit juices stored in cans, leak- proof bags, and tetraPaks). Methodology: The study utilized cell lines, namely HepG2 (human liver cancer) and HeK293 (human kidney), to assess the impact of these heavy metals on cellular viability, DNA damage, mitochondrial function, and cell cycle progression. Flow cytometry was used to analyse cell cycle alterations. Results: The MTT assay showcased dose-dependent decrease in cell viability with increasing concentrations of heavy metals. Annexin V Assay determined V, Hg and As had necrotic death pattern while Cd had apoptotic death pattern for both the cell lines in presence of heavy metal digests. Cells treated with heavy metals, and the resulting DNA damage observed to be high after quantified using comet tail length, olive tail movement, and tail moment measurements. Mitochondrial function assays in the presence of heavy metals revealed that increased mitochondrial membrane potential, mitochondrial mass, and reactive oxygen species production. ROS generation assessed to understand the impact of heavy metals on cellular health and oxidative stress. Conclusion: This study shed light on the potential cytotoxic effects of heavy metals found in fruit juices. These findings contribute to our understanding of the potential risks associated with heavy metal exposure from consumer products and emphasize the importance of regulatory measures to ensure food safety and human health.

Microbial Quality of Fresh Juices Sold by Street Vendors and Associated Human Health Risks: The Case of Ilala Ward, Dar es Salaam City, Tanzania

Microbial food quality serves to indicate health risks associated with the consumption of contaminated foods. This study aimed to determine the microbial quality of fresh juices and the level of awareness and perception of the consumers and juice vendors at Ilala Ward, Dar es Salaam. Fresh samples of sugarcane, mango and mixed fruit juices were collected from four different sampling stations and analysed using standard methods for Total Coliform (TC), Faecal Coliform (FC) and Total Viable Count (TVC). The level of awareness and perception of 60 juice vendors and 60 consumers were also assessed by using questionnaires. The results showed that TC numbers ranged from the lowest mean of 6.100±4.180x106 to 7.483±3.650x107 MPN/ml, FC ranged from 2.566±0.989x106 to 2.017±1.687x107 MPN/ml while TVC ranged from 5.046±1.032x109 to 5.871±0.835x109 CFU/ml of fresh juices. The level of TC, FC and TVC was beyond the recommended international and Tanzanian standards showing that fresh juices posed a high risk for consumers. On the other hand, 42% of the juice consumers had low awareness of microbial contamination of fresh juices, which leads to high health risks. It is recommended that street juice vendors should adopt proper hygiene measures and undergo appropriate training on microbial quality, food safety and sanitation.

Oxidized carboxymethyl cellulose/polyaniline-based hybrid nanocomposite for sensitive detection of environmentally hazardous nitrophenol in real samples

Present work aims at fabricating chemically active, reproducible, and reliable electrode material by Ag nanoparticles anchored oxidized-carboxymethyl cellulose (O-CMC) embedded Pani (Ag@Pani/O-CMC) based nanocomposite for the trace detection of 4-nitrophenol. The Ag@Pani/O-CMC was physiochemically characterized in detail using FTIR, SEM, TEM, UV–vis, XPS, and XRD to analyze functional groups, structural morphology, optical properties, and chemical compositions. Differential pulse voltammetry and cyclic voltammetry were adopted to explore the electrochemical performance of Ag@Pani/O-CMC modified glassy carbon electrode (Ag@Pani/O-CMC/GCE). The observed reduction peak current was proportional to the 4-NP concentrations with a high sensitivity (2.48 µA nM−1 cm−2) and displayed a detection limit of 0.58 nM and a good linear range of 10–100 nM. In addition, the sensor exhibited comparable sensing capability in real biological samples, including milk, mixed fruit juice, and tap water, with excellent recovery from 97.20 % to 101 %. It also depicted high repeatability as the current response remained at 84.52 % after 28 days of operation for 4-NP detection. This approach can be projected to result in the electrochemical sensor for safe industrial processing and environmental monitoring, which is expected to have good stability, excellent selectivity, and sensitivity for detecting traces of 4-NP in actual samples.

An accurate and sensitive determination of selected pesticides in mixed fruit juice samples using the combination of a simple and efficient microextraction method and GC-MS with a matrix matching calibration strategy.

Pesticides have been used on several fruits, vegetables and cereals to control harmful organisms in order to increase the quality of products; however, these substances cause serious health effects. Therefore, an accurate and sensitive analytical method should be developed for the determination of pesticides to evaluate their toxicity. In this study, an efficient microextraction strategy was applied to preconcentrate eight different selected pesticides from mixed fruit juice samples prior to gas chromatography-mass spectrometry detection. All significant parameters such as spraying number, extraction solvent type, sample volume and mixing type/period belonging to the developed extraction method were elaborately optimized to get low detection limits. After the optimization studies, system analytical performance studies were carried out and limit of detection (LOD) values varied from 0.04 μg /kg-1 to 1.99 μg kg-1 (mass based) for the selected analytes. Under the optimum experimental conditions, spiking recovery experiments were performed in the mixed fruit juice samples to evaluate the applicability and accuracy of the proposed method. The recovery results were recorded in the range of 81.4-123.5% with acceptable standard deviations by applying a matrix matching calibration strategy. The proposed analytical method can be used for the qualitative and quantitative determination of selected pesticides in the mixed fruit juice samples and can also be applied to other fruit juice samples using a matrix matching calibration strategy.

Photostability of vitamin C in industrialized fruit juices and isomers determination by HPLC-DAD

L-ascorbic acid (LAA) and D-isoascorbic acid (DIAA) are two of the most important antioxidants in industrialized fruit products as mixed fruit juices, fruit concentrates, and jams, in which they are added as antioxidants and as a nutrient. Despite that, LAA may undergo oxidative- and photo-degradation during storage, compromising its concentration and activities in these food products. In this work we aimed to optimize a method for the identification and quantification of LAA and DIAA by high performance liquid chromatography with diode-array detection (HPLC-DAD) using a NH2 column and a pre-heated mobile phase. A further aim was to investigate if food packing actually protects fruit juices against LAA photodegradation. For the UV photostability test, samples were UV-irradiated at 1416 μW cm−2, at 28 ± °C, and with a radiation energy above 200 W h m−2. The optimized HPLC method provided complete peak resolution of vitamin C isomers in less than nine min run-time. At the end of photostability tests with fruit juices and jellies packaged in polyethylene terephthalate and glass bottles or flasks, L-ascorbic acid was undetectable, suggesting that the packages did not provide effective protection against vitamin C photodegradation. To the best of our knowledge, vitamin C photostability in fruit products was previously unreported.

Production of a mixed fruit juice powder using mixture analysis and a fuzzy model

The present work aimed to find a mixed fruit juice powder with high antioxidant activity and sensory score. A two-step fuzzy algorithm and fuzzy toolbox were applied to produce acceptable sensory data for the mixture analysis design. The mixture design was then analysed using two responses of sensory and antioxidant activity, and the optimised beverage consisted of red grape (20.4%), mulberry (75.6%), and strawberry (4.0%). As compared to single fruit juices, the selected mixture yielded high content of phenolic compounds with desirable antioxidant activity and sensory score. Spray- and freeze-drying were then compared for the fruit juice powder production from selected mixed juices formulated with 20% maltodextrin (DE = 20). A significant (p < 0.05) difference was observed between the powder yield (82.0% in comparison to 51.7%), free radical scavenging activity (85.6% in comparison to 75.4%), and total phenolic content (2958.8 in comparison to 2791.4 mg GAE/L) of the freeze- and spray-dryer, respectively. Furthermore, the spray-dried powder was much lighter than the freeze-dried powder, with a lower chroma index, redness, and higher hue angle. Overall, freeze-drying was less destructive than spray-drying in the powder production from the mixed juice.

Short-Term Effects of Fruit Juice Enriched with Vitamin D3, n-3 PUFA, and Probiotics on Glycemic Responses: A Randomized Controlled Clinical Trial in Healthy Adults.

This study aimed to determine the glycemic index (GI) of a commercial mixed fruit juice (apple, orange, grape, and pomegranate; FJ) fortified with vitamin D3 or n-3 polyunsaturated fatty acids (PUFA) or probiotics, and their combination, and their effects on glycemic responses and salivary insulin concentrations. In a randomized controlled, double-blind, crossover study, 11 healthy participants (25 ± 2 years; five women; body mass index = 23 ± 1 kg/m2) were randomly assigned to receive five types of FJs [vitD (with vitamin D3); n-3 (with n-3 PUFA); probiotics (with Lacticaseibacillus casei Shirota and Lacticaseibacillus rhamnosus GG); vitD-n-3-probiotics FJ (combination of vitD3-n-3-probiotics), control (regular FJ)], all containing 50 g available carbohydrate, and glucose as reference drink. All FJs provided low GI values (control: 54; vitD3: 52; n-3: 51; probiotics: 50; and vitD-n-3-probiotics combination: 52, on glucose scale). Compared to the FJ control, the enriched FJs produced different postprandial glycemic and insulinemic responses and affected satiety scores. All FJ types, regardless of the added biofunctional ingredients, attenuated postprandial glycemic responses, which may offer advantages to glycemic control.

Speedy Gonzales analysis method in one drop of beverage, for crimes committed with drugs from three different groups.

Sertraline, zolpidem and fentanyl, are drugs with potential to be used in cases of rape, property theft and organ theft. In this study, a 15min dilute-and-shoot analysis method was developed for the simultaneous confirmation and quantification of these drugs, using liquid chromatography tandem mass spectrometry (LC-MS/MS), in the residues of fruit juice types (mixed fruit juice, cherry juice and apricot juice), frequently consumed soft drinks. A C18phenomenex column (3µm × 100mm × 3mm) was used in LC-MS/MS analysis. Validation parameters were determined by means of linearity, linear range, LOD, LOQ, repeatability and intermediate precision studies. The linearity of the method was shown up to 2.0 μgmL-1 concentration and r2 was ≥ 0.99, for each analyte. LOD and LOQ values were found in the ranges of 4.9-10.2 and 13.0-57.5 ngmL-1 for all the analytes. Accuracies were between 74 and 126%. HorRat values calculated (between 0.57 and 0.97), revealed that the inter-day precisions (RSD% ≤ 15.5%) are acceptable. The simultaneous extraction and determination of these analytes in beverage residues in very low amounts as 100 µL is challenging because of the difficulty arising from the different chemical properties, the complexity of mixed fruit juice matrix. The method is important for hospitals (especially in emergency-toxicology cases), criminal and special laboratories from the point of determining the combined or single use of these drugs in drug facilitated crimes (DFC) and finding out the reasons of deaths related to these drugs.

Assessment of Nutritional Quality and Physico-Chemical Properties of Semi-Commercially Produced Mixed Fruit Juice From Citrullus lanatus, Citrus sinensis and Citrus paradise

Nutritional quality, a value added measure has recently been emphasized as an important consideration in the production of commercially available fruit juices in order to promote health and wellness of the consumers. Therefore, this study was designed to investigate the nutritional value of a laboratory produced mixed fruit juice using fresh samples of water melon, Citrullus lanatus; grape, Citrus paradisi and sweet orange, Citrus sinensis, under strict cleanliness. The microbiological assessment of fruit juices was carried out and raw untreated fruit juices were all coliform free. The mixed fruit juice had a pH value of 4.31, acidity; 5.30 g/ kg tartaric acid; specific gravity of 1.06 and organoleptic values (low), respectively. Pasteurization for 30 min at 75 0C with added 40 ppm of sodium metabisulphite apparently optimized the product, being highly microbiologically stable. On the basis of combined favourable organoleptic, physicochemical and microbiological properties, this product could be suggested suitable for human consumption and therefore, recommended for commercial purposes. How to Cite: Agunbiade Shadrach Oludare, Afolabi Olakunle Bamikole, Mabayoje Samson Olatunde, Ogunleye Omotola, Jaiyesimi Kikelomo Folake, 2023. "Assessment of Nutritional Quality and Physico-Chemical Properties of Semi-Commercially Produced Mixed Fruit Juice From Citrullus lanatus, Citrus sinensis and Citrus paradise." Journal of Agriculture and Crops, vol. 9, pp. 205-210.

Hybridized sulfated-carboxymethyl cellulose/MWNT nanocomposite as highly selective electrochemical probe for trace detection of arsenic in real environmental samples.

A highly selective and ultra-sensitive electrochemical sensing probe was proposed by combining sulfated-carboxymethyl cellulose (CMC-S) and a functionalized-multiwalled carbon nanotube (f-MWNT) nano-composite with high conductivity and durability. The CMC-S/MWNT nanocomposite was impregnated on a glassy carbon electrode (GCE) to construct the non-enzymatic and mediator-free electrochemical sensing probe for trace detection of As(iii) ions. The fabricated CMC-S/MWNT nanocomposite was characterized by FTIR, SEM, TEM, and XPS. Under the optimized experimental conditions, the sensor exhibited the lowest detection limit of 0.024 nM, a high sensitivity (69.93 μA nM-1 cm-2) with a good linear relationship in the range of 0.2-90 nM As(iii) concentration. The sensor demonstrated strong repeatability, with the current response continuing at 84.52% after 28 days of use, in addition to good selectivity for the determination of As(iii). Additionally, with recovery ranging from 97.2% to 107.2%, the sensor demonstrated comparable sensing capability in tap water, sewage water, and mixed fruit juice. The electrochemical sensor for detecting trace levels of As(iii) in actual samples is anticipated to be produced by this effort and is expected to possess great selectivity, good stability, and sensitivity.

Physicochemical and Sensory Characteristics of Mixed Fruit Juices Prepared from Blend of Pineapple, Pawpaw and Watermelon Fruits Juices

This study investigated the production of mixed fruit juices from pawpaw, watermelon and pineapple fruits, pawpaw and watermelon fruits. Fresh ripe fruits (pineapple, watermelon and pawpaw and watermelon) were washed, manually peeled, deseeded, sliced, squeezed and sieved to obtain pineapple, watermelon and pawpaw juice pawpaw and watermelon juice. The prepared juices were pasteurized at 85°C for 15 s,sec., packaged in air-tight plastic cans. The pineapple, watermelon and pawpaw juice and watermelon juice were blended in ratios of 100:0:0;0:100:0; 0:100:0; 20:20:60:20; 33.33:33.33:33.33; 60:20:20 and 40:30:30%. The juice samples were evaluated for physicochemical and sensory characteristics using standard procedures. The sensory results showed colour rating of (6.33 - 7.22), flavour (6.11 – 7.78), taste (6.44 – 7.78), and general acceptability of(6.89 – 8.00). Sample 100% PIN was rated the best (8.00) in general acceptability. However, all the juice samples were generally accepted. The physicochemical results showed the following range of values for titrabletitra table acidity (0.60 - 1.21%), total soluble solids (8.10 - 15.55%), total sugar (7.22 - 9.51%), pH (4.05 - 5.30) and vitamin C (4.80-17.00 mg/100g). Generally, the juice samples were within the regulatory specifications, and acceptable. The study showed that fruit juice with good physicochemical and sensory qualities could be formulated using pineapple, watermelon and using pineapple, pawpaw and watermelon blend. The formulated fruit juices could find domestic and industrial applications.

Formula Optimization of Black Mulberry and Canistel Mixed Juice using Mixture D-Optimal from Design Expert Software

Black mulberry (Morus nigra L.) and canistel (Pouteria campechiana) are classified as fruit commodities that are still not optimal in their utilization. Based on their sensory and nutritional characteristics, they have potential to be processed into products for a longer shelf life and more valuable. The purpose of this study was to determine the optimal formula for a mixed fruit juice product made from black mulberry and canistel in terms of chemical, physical and sensory characteristics. The optimization formula was analyzed by Design Expert software ver.11 and through the Mixture D-Optimal method. The independent variables in this study are black mulberry puree, canistel puree, and sucrose. The dependent variables were 0.05% sodium benzoate and a mixture of CMC and sodium alginate 0.25% with a ratio of CMC: sodium alginate 1:1. The chemical parameters analyzed consisted of anthocyanin, beta carotene, vitamin C, and antioxidant activity. The physical parameters consisted of viscosity and sensory parameters consisted of flavor, sweet taste, and sour taste. The results obtained that the optimal formulation was based on the highest desirability value from the results of the analysis using the Design Expert program and the value was 0.807. The optimal formula for mixed fruit juices was black mulberry puree 53.356%, canistel puree 35.252%, and sucrose 11.092%.

Effects of Probiotic Fermented Fruit Juice-Based Biotransformation by Lactic Acid Bacteria and Saccharomyces boulardii CNCM I-745 on Anti-Salmonella and Antioxidative Properties.

Fermentation is an effective process for providing various beneficial effects in functional beverages. Lactic acid bacteria and yeast fermentation-based biotransformation contribute to enhancement of nutritional value and digestibility, including lactose intolerance reduction and control of infections. In this study, the probiotic fermented fruit juice (PFJ) was produced by Lactobacillus plantarum TISTR 1465, Lactobacillus salivarius TISTR 1112, and Saccharomyces boulardii CNCM I-745 while mixed fruit juice (MFJ) was used as the basic medium for microorganism growth. The potential function, the anti-salmonella activity of PFJ, was found to be effective at 250 mg/ml of MIC and 500 mg/ml of MBC. Biofilm inhibition was performed using the PFJ samples and showed at least 70% reduction in cell attachment at the MIC concentration of Salmonella Typhi DMST 22842. The antioxidant activities of PFJ were determined and the results revealed that FSB.25 exhibited 78.40 ± 0.51 mM TE/ml by FRAP assay, while FPSB.25 exhibited 3.44 ± 0.10 mM TE/ml by DPPH assay. The volatile compounds of PFJ were characterized by GC-MS, which identified alcohol, aldehyde, acid, ester, ketone, phenol, and terpene. The most abundant organic acid and alcohol detected in PFJ were acetic acid and 2-phenylethanol, and the most represented terpene was β-damascenone. The sensory attributes showed scores higher than 7 on a 9-point hedonic scale for the FPB.25, illustrating that it was well accepted by panelists. Taken together, our results showed that PFJ could meet current consumer demand regarding natural and functional, fruit-based fermented beverages.

Total fructose, total glucose and benzoic acid contents in commercially available fruit nectars in Anuradhapura town area, Sri Lanka

High intake of total fructose, total glucose and benzoic acid can cause many adverse health effects. Most commercially available fruit juice and nectar brands in Sri Lanka are not claimed on the label about the levels of fructose, glucose and benzoic acid in the products. Available brands of fruit juices [Mixed Fruit Juice (MFJ) and Mango Juice (MJ)] and fruit nectars [Mixed Fruit Nectar (MFN) and Mango Nectar (MN)] were selected through a pre-validated questionnaire. Seven brands of MN and MFN were identified as mostly consumed and there was a significant difference (p<0.05) in the total fructose and total glucose levels among the brands. Total fructose of MN and MFN was in the range of 28.54 ± 3.03 gl-1 to 60.28 ± 5.04 gl-1 and 30.46 ± 8.56 gl-1 to 62.47 ± 9.27 gl-1 respectively. Total glucose content of MN was in the range of 17.49 ± 5.39 gl-1 to 41.87 ± 2.11 gl-1 and for MFN from 29.29 ± 4.34 gl-1 to 85.71 ± 5.64 gl-1. Total fructose in MJ and MFJ were in the range of 32.28 ± 0.86 gl-1 to 37.66 ± 0.55 gl-1 and 33.14 ± 0.87 gl-1 to 36.81 ± 1.81 gl-1 respectively. Three, out of the four brands of MN and MFN contained benzoic acid. One brand of MN and one brand of MFN had exceeded the permitted level (120 mg/l) of benzoic acid. Benzoic acid was present in two, out of three brands of MJ and MFJ. It can be concluded that there is a variation in total fructose, total glucose and benzoic acid contents in fruit nectars and juices available for sale in Anuradhapura town area. However, some brands have violated the Sri Lanka food regulations.