Mongrel Research Articles

In vivo mosquito repellency effect of citronella (Cymbopogon nardus (L.) Rendle) essential oil bath bomb formulation in dogs.

Mosquitoes carry numerous diseases of medical and veterinary significance. While citronella essential oil is safe as a mosquito repellent, extensive research does not document its ability to deter mosquitoes from animals. This study assessed the citronella essential oil bath bomb's ability to repel Culex quinquefasciatus mosquitoes in dogs. Citronella essential oil's chemical composition was analyzed using gas chromatography-mass spectrometry (GC-MS). Through freeze-thaw testing, a bath bomb formulation containing 6% w/w citronella essential oil was assessed for its physical and chemical stability. Thirty-two healthy client-owned mixed-breed dogs were employed to test the mosquito-repellency effects of citronella essential oil (treatment group) and olive oil (control group) bath bomb formulations. Bath bombs were tested for irritation effects on animal skin for 15-day post-application. Thirty-six compounds were identified through GC-MS, with citronellal (23.38%), δ-cadinene (12.25%), and geraniol (9.09%) being the most prevalent constituents. The bath bomb maintained its original physical properties after undergoing six freeze-thawing cycles and retained over 90% of its citronella essential oil. About 100%, 69.28%, and 65.58% mosquito repellency were displayed by the citronella essential oil bath bomb at 3 h, 6 h, and 8 h, respectively. None of the test animals exhibited skin irritation during the study. The citronella bath bomb effectively repelled C. quinquefasciatus in dogs without irritating their skin. The formulation's physical and chemical stability is demonstrated by the results of freeze-thaw stability testing. Further studies should be conducted to evaluate the repelling activity against other mosquito species.

Aspectos anatomopatológicos de um tumor misto maligno de tireoide com múltiplas metástases em cão

Thyroid tumors are uncommon. Malignant mixed tumor, or carcinosarcoma, is a rare, malignant, infiltrative, and metastatic neoplasm. This paper aims to report a case of a metastatic malignant mixed thyroid tumor in a 10-year-old mixed-breed female dog. The patient presented respiratory symptoms and had an unfavorable evolution of the clinical scenario. It was euthanized and submitted to necroscopic examination, which led to subsequent histopathological and immunohistochemical (IHC) evaluation. The macroscopic evaluation revealed neoformations in the thyroid, lung, heart, kidneys, and abdominal skeletal muscles. Microscopically, the thyroid presented mixed neoplastic cellularity as well as mesenchymal and epithelial cells. Metastases of the mesenchymal component were observed in the kidneys, heart, and musculature, while there was an invasion of neoplastic epithelial cells in the lung. On the IHC, there was positive staining for thyroglobulin, TTF-1, and vimentin. The histopathological and IHC findings indicated a metastatic malignant mixed thyroid neoplasm.

Tolerability of 2 and 4 mg/kg Dosing Every 12 Hour of a Cannabidiol- and Cannabidiolic Acid-Rich Hemp Extract on Mixed-Breed Dogs Utilized for Teaching in a Closed Colony.

With the increase in popularity of utilizing cannabidiol (CBD) for human ailments, owners are actively interested in the possible utilization of cannabinoid products for their pets. The evaluation of CBD-rich hemp as an anti-anxiolytic, anti-inflammatory, immunomodulator, and anti-epileptic supplement has been assessed in previous studies in dogs, with adverse events such as ataxia or lethargy noted. In this study, the utilization of CBD-rich hemp was assessed at two concentrations to ascertain the impact on behavior as well as the tolerability of the medication given in a typically recommended dose and then twice that dose. Eighteen dogs were utilized in a randomized, double-blinded, placebo-controlled, 3 × 3 designed study. Each group of six dogs was provided placebo, 2 mg/kg, and 4 mg/kg of a cannabidiol/cannabidiolic acid (CBD/CBDA)-rich hemp in two-week intervals with one-week washout periods between each treatment period. Throughout the 10-week treatment period, student evaluations were performed, simulating clients' subjective assessments. Improvements in anxiety-related behavior and adverse events related to lethargy and ataxia were not observed and may indicate that the utilization of CBD-rich hemp products for behavioral changes may require higher dosing to mitigate unwanted behaviors in normal, healthy dogs. Furthermore, serum chemistry and serum cortisol were evaluated after each treatment period showing only a mildly significant increase in serum alkaline phosphatase when dosing at 4 mg/kg every 12 h, which is consistent with previously reported CBD dosing at these higher concentrations. Adverse events associated with CBD/CBDA-rich hemp extract given at 2 and 4 mg/kg every 12 h for two weeks were not reported, suggesting that using CBD-rich hemp in young, healthy dogs was safe during two weeks of treatment.

Effects of hard- and/or soft-tissue grafting with early implant placement: Histomorphometric outcomes of an exploratory study in canines.

To histomorphometrically determine the effects of hard- and/or soft-tissue augmentation by applying the early implant placement protocol. The distal roots of the maxillary second and third premolars were extracted in 10 mongrel dogs. After 1 month, early implant placement was performed in the distal roots, resulting in buccal dehiscence defects. Four treatments were then randomly applied: (1) guided bone regeneration (GBR), (2) connective tissue grafting (CTG), (3) simultaneous GBR and CTG (GBR + CTG) and (4) no further treatment (control). Healing abutments were connected to all implants to allow non-submerged healing. At 4 and 16 weeks, tissue sections were harvested and histomorphometric analyses were performed. Group GBR presented the largest total tissue thickness at 4 weeks, but underwent a greater remodelling compared with the other groups between 4 and 16 weeks. At 16 weeks, the overall tissue thickness was largest in group GBR + CTG. This group was also the most favourable in terms of the level of the margo mucosae and the presence of mineralized tissue at the coronal level. Group CTG demonstrated a stable tissue thickness over time, with a larger thickness at 16 weeks compared with group GBR. Simultaneous CTG and GBR resulted in the most-favourable tissue thickness when applying the early implant placement protocol.

Surgical management of unilateral restrictive strabismus in an 8-month-old dog

ABSTRACT Case history An 8-month-old male, entire, mixed-breed dog was presented with a 1-month history of left exophthalmos and green mucopurulent ocular discharge. Subsequently, exophthalmos resolved but esotropia (medial strabismus) developed in the left eye, prompting referral to an ophthalmologist. Clinical findings At the initial referral consultation, enophthalmos and esotropia of the left eye were identified. The patient showed mild improvement after a 3-week tapering course of oral prednisolone and doxycycline. MRI was performed and showed left medial rectus muscle atrophy with increased contrast enhancement which was consistent with chronic extraocular muscle myositis (EOM). A forced duction test was performed to confirm the diagnosis of fibrosing esotropia, which is likely a sequela of chronic EOM. Diagnosis Fibrosing esotropia presumably caused by untreated EOM. Treatment and outcome One month later, esotropia progressed to a marked ventro-medial strabismus resulting in visual deprivation. Surgical release of the ventral oblique, medial and ventral recti muscles was performed, resulting in immediate resolution of the enophthalmos. Despite a tapering post-operative course of oral prednisolone, mild esotropia was present 4 weeks later. In an effort to stabilise the globe position, the low dose of prednisolone was increased to a higher anti-inflammatory dose before slowly tapering over 2 months. The vision in the left eye was improved after surgery and has been maintained since without further treatment. Clinical relevance This is the first documented case of fibrosing esotropia in a young dog with prior signs of acute exophthalmos. Fibrosing esotropia has been documented in certain breeds or as a sequela to chronic EOM. In this patient, it was presumably caused by EOM, which was strongly supported by the case history, progression and MRI findings. Most historical reports of EOM described it as a bilateral condition that resolves with systemic corticosteroids at an anti-inflammatory dose. EOM has been shown to also present unilaterally and it can progress to strabismus if not promptly recognised and treated with systemic steroids. Surgical management can restore vision when severe strabismus results in visual deprivation.

Platelet-rich plasma attenuates the UPEC-induced cystitis via inhibiting MMP-2,9 activities and downregulation of NGF and VEGF in Canis Lupus Familiaris model

One of the most prevalent disorders of the urinary system is urinary tract infection, which is mostly brought on by uropathogenic Escherichia coli (UPEC). The objective of this study was to evaluate the regenerative therapeutic and antibacterial efficacy of PRP for induced bacterial cystitis in dogs in comparison to conventional antibiotics. 25 healthy male mongrel dogs were divided into 5 groups (n = 5). Control negative group that received neither induced infection nor treatments. 20 dogs were randomized into 4 groups after two weeks of induction of UPEC cystitis into; Group 1 (control positive; G1) received weekly intravesicular instillation of sodium chloride 0.9%. Group 2 (syst/PRP; G2), treated with both systemic intramuscular antibiotic and weekly intravesicular instillation of PRP; Group 3 (PRP; G3), treated with weekly intravesicular instillation of PRP, and Group 4 (syst; G4) treated with an intramuscular systemic antibiotic. Animals were subjected to weekly clinical, ultrasonographic evaluation, urinary microbiological analysis, and redox status biomarkers estimation. Urinary matrix metalloproteinases (MMP-2, MMP-9) and urinary gene expression for platelet-derived growth factor -B (PDGF-B), nerve growth factor (NGF), and vascular endothelial growth factor (VEGF) were measured. At the end of the study, dogs were euthanized, and the bladder tissues were examined macroscopically, histologically, and immunohistochemically for NF-κB P65 and Cox-2. The PRP-treated group showed significant improvement for all the clinical, Doppler parameters, and the urinary redox status (p < 0.05). The urinary MMPs activity was significantly decreased in the PRP-treated group and the expression level of urinary NGF and VEGF were downregulated while PDGFB was significantly upregulated (p < 0.05). Meanwhile, the urinary viable cell count was significantly reduced in all treatments (P < 0.05). Gross examination of bladder tissue showed marked improvement for the PRP-treated group, expressed in the histopathological findings. Immunohistochemical analysis revealed a marked increase in Cox-2 and NF-κB P65 in the PRP-treated group (P < 0.05). autologous CaCl2-activated PRP was able to overcome the bacterial infection, generating an inflammatory environment to overcome the old one and initiate tissue healing. Hence, PRP is a promising alternative therapeutic for UPEC cystitis instead of conventional antibiotics.

Morphometric assessment of the choroid in dogs diagnosed with retinal atrophy (RA) with symptoms of progressive retinal atrophy, using spectral-domain optical coherence tomography (SD-OCT)

The aim of the study was to determine the thickness of choroidal layers in mixed breed dogs suffering from retinal atrophy (RA) and showing symptoms of progressive retinal atrophy (PRA), with the use of SD-OCT. The study was performed on 50 dogs divided into two groups: 25 dogs diagnosed with retinal atrophy (RA) with PRA symptoms aged 1.5-14 years and 25 healthy dogs aged 2-12 years. The dogs were examined using slit-lamp biomicroscopy, tonometry, ophthalmoscopy, fundus camera and SD-OCT (Topcon 3D OCT 2000). Measurements of the choroidal layers: RPE-Bruch membrane-choriocapillaris complex (RPE-BmCc) with tapetum lucidum in tapetal fundus, medium-sized vessel layer, (MSVL), large vessel layer with lamina suprachoroidea and (LVLS) whole choroidal thickness (WCT) were taken manually with the use of the caliper function integrated with the SD-OCT software. The measurements were performed dorsally (D) and ventrally (V) at a distance of 5000-6000 μm, and temporally (T) and nasally (N) at a distance of 4000-7000 μm from the optic disc with enhanced depth scans. The measurements were conducted temporally and nasally both in the tapetal (TempT, Nast) and nontapetal (TempNT, NasNT) fundus. Statistical analysis was performed using Statistica 10 software (Mann Whitney U Test). In all dogs affected by retinal atrophy (RA) with PRA symptoms, a statistically significant (p≤0,05) reduction in thickness of MSVL was observed in all the measured regions. A statistically significant reduction in thickness of LVLS and WCT was found in all nontapetal areas (p≤0,05). RA in mixed breed dogs with PRA symptoms was accompanied by choroid disorders such as reduction in thickness of the large vessel layer and decreased whole choroidal thickness in the nontapetal fundus as well as the medium vessel layer in all fundus regions.

Effect of sex on the levels of total arsenic (As) and As(III) in dog urine: a preliminary study

The aim of the study was to analyze differences in the concentration of total arsenic (As) and As(III) in urine depending on the sex of mixed-breed dogs. Therefore, a research hypothesis was put forward that sex is a variable determining the degree and efficiency of urinary arsenic excretion. Two study groups were established: female (group 1) and male (group 2) mixed-breed dogs of similar body weight (9-13 kg) and aged 8-11 years. Urine samples were collected using a device designed specially for this purpose (utility model registered at the Patent Office of the Republic of Poland, no. WUP 13/2023). Samples were wet-digested following the protocol presented in the PN-EN 13805:2014 standard and analysed using an EcaFlow 150 GLP coulometer integrated with an E-53 Au electrode and EcaCell. Arsenic content in dog food was also measured to verify the effect of this variable. Results were analyzed using Statistica 13.1 software. Sex had a significant effect on the urinary arsenic excretion. The levels of total As and As(III) were significantly higher in urine from male dogs (18.45 and 2.92 [μg L-1]; p≤0.05) with compared to urine from female dogs (13.43 and 1.67 [μg L-1], respectively).

Short-Term Effects of Two COX-2 Selective Non-Steroidal Anti-Inflammatory Drugs on the Release of Growth Factors and Cytokines from Canine Platelet-Rich Gel Supernatants.

(1) Background: There is a lack of knowledge about how a single dose of COX-2 selective non-steroidal anti-inflammatory drugs (NSAIDs) might affect the release of growth factors (GFs) and cytokines from canine platelet-rich gels (PRGs) and other hemocomponents. (2) Methods: A crossover study was conducted in six adult mongrel dogs. Animals were randomized to receive a single dose of either carprofen or firocoxib. PRG, temperature-induced platelet lysate (TIPL), chemically induced PL (CIPL), and plasma hemocomponents were obtained from each dog before (1 h) and after (6 h) the treatments. Platelet and leukocyte counts and determination of the concentrations of platelet-derived growth factor-BB, (PDGF-BB), transforming growth factor beta-1 (TGF-β1), interleukin 1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α) and IL-10 concentrations were assayed by ELISA in all hemocomponents. (3) Results: Both platelet and leukocyte counts and PDGF-BB concentrations were not affected by NSAIDs and time. Total TGF-β1 concentrations were not affected by NSAIDs; however, the release of this GF was increased in PRG supernatants (PRGS) at 6 h. IL-1β and TNF-α concentrations were significantly (p < 0.001) lower in both firocoxib PRGS and plasma at 6 h, respectively. IL-10 concentrations were significantly (p < 0.001) lower at 6 h in all hemocomponents treated with both NSAIDs. (4) Conclusions: The clinical implications of our findings could indicate that these drugs should be withdrawn from patients to allow their clearance before the clinical use of PRP/PRG. On the other hand, the prophylactic use of NSAIDs to avoid the inflammatory reactions that some patients might have after PRP/PRG treatment should be performed only in those animals with severe reactive inflammation to the treatment.

Photobiomodulation therapy using a class 3b laser does not cause premature rupture of multivesicular liposomal bupivacaine suspensions in a cadaver model.

To determine if photobiomodulation causes a premature release of liposomal bupivacaine (LB) suspensions. A 25-kg mixed breed dog cadaver euthanized for reasons unrelated to this study. In September 2022, a proximomedial tibial incision was made in a dog cadaver, and a tibial plateau leveling osteotomy plate was implanted. A stab incision was made one-half inch distal to the incision, and a tunnel to the plate was created prior to closure of the primary wound. A 3-cc anal sac catheter was advanced through the distal incision until the bulb rested against the face of the plate. Seven treatment groups of treatment power (watts) and total energy (joules/cm2) were defined as: A, 0.5 W, 2.0 J/cm2; B, 0.5 W, 4.0 J/cm2; C, 0.5 W, 6.0 J/cm2; D, 1.0 W, 2.0 J/cm2; E, 1.0 W, 4.0 J/cm2; F, 1.0 W, 6.0 J/cm2; and sham, 0.0 W, 0.0 J/cm2. Ten samples per group of 2 mL of LB were infused into a new catheter and treated percutaneously with a class 3b laser. All samples remained in the catheter for 12 seconds to reflect the longest treatment time. Post-treatment free bupivacaine concentrations were identified with high-performance liquid chromatography. The median free bupivacaine concentration was reported as: sham, 1.89 mg/mL; A, 1.93 mg/mL; B, 2.01 mg/mL; C, 2.05 mg/mL; D, 1.92 mg/mL; E, 2.03 mg/mL; and F, 2.00 mg/mL. There were no differences in median free bupivacaine concentrations between groups (P = .988). Concurrent LB and photobiomodulation are recommended during the postoperative period. The results of this proof-of-concept study suggest that concurrent use of LB and photobiomodulation may be safe, but in vivo studies at similar and stronger photobiomodulation settings are warranted.

Effect of body position on transesophageal atrial pacing in dogs

ObjectiveTo evaluate the effect of body position on transesophageal atrial pacing (TEAP) in dogs. Study designProspective, experimental trial. AnimalsA total of eight mixed-breed dogs, median body mass 25 kg (range 11–31 kg). MethodsTEAP was performed using a 6 Fr (2 mm) pacing catheter introduced transorally and positioned in the lower esophagus in left lateral recumbency. The catheter was withdrawn in 5 mm increments until successful TEAP. The minimum pacing threshold (MPT) was determined at each 5 mm increment until atrial capture could not be achieved at a maximal pulse amplitude of 40 mA. The zone of capture (ZOC) was the total distance over which TEAP was achieved. The same process was repeated in right lateral, ventral and dorsal recumbency. A linear mixed model was used to assess the impact of body position on TEAP and ZOC. ResultsTEAP was achieved in all body positions. There was significant random effect of individual dogs on ZOC (p = 0.048) but not on MPT (p = 0.109). MPT was 11.56 ± 8.65 mA (mean ± SD), 8.43 ± 2.29 mA, 10.63 ± 6.91 mA and 10 ± 7.44 mA for dorsal, left and right lateral, and ventral recumbency, respectively. ZOC was 5.12 ± 2.08 cm, 6.35 ± 2.41 cm, 5.94 ± 3.23 cm and 5.31 ± 2.2 cm for dorsal, left lateral and right lateral, and ventral recumbency, respectively. Neither ZOC (p = 0.363) nor MPT (p = 0.716) varied significantly between body positions. Conclusions and clinical relevanceTEAP can be performed successfully in all body positions in dogs. Body position did not significantly influence MPT or ZOC values for TEAP. Further studies are needed to compare the efficacy of TEAP with the efficacy of standard pharmacological therapy for management of bradycardia during general anesthesia in dogs.

Granulocytic neoplasm suggestive of primary myeloid sarcoma in 3 dogs.

Myeloid sarcoma (MS) is a solid tumor of granulocytic origin with extramedullary localization. This tumor is rare in humans and animals. The diagnostic approach is heterogeneous, and the definitive diagnosis may be difficult to achieve. Primary MS has never been described as a spontaneous neoplasm in companion dogs. Two purebred and 1 mixed-breed dogs, 6- to 11-year-old, developed round cell tumors in the mediastinum, lymph nodes (LNs) and tonsils, and LNs, respectively. Granulocytic origin and exclusion of lymphoid lineage were confirmed by flow cytometry, supported by immunohistochemistry or immunocytochemistry. Pivotal to the diagnosis were positive labeling for myeloid (CD11b, CD14) and hematopoietic precursors (CD34) markers, along with negative labeling for lymphoid markers. Blood and bone marrow infiltration were not detected at initial diagnosis, excluding acute myeloid leukemia. The behavior of these tumors was aggressive, resulting in poor clinical outcomes, even when chemotherapy was attempted.

Synovial myxosarcoma in the scapulohumeral joint of a dog: a case report.

A 12-year-old, 3kg spayed female mixed-breed dog was evaluated to assess a 1-year history of intermittent right forelimb lameness that did not have adequate response to nonsteroidal anti-inflammatory drugs. The radiographic study performed under sedation showed multifocal radiolucent areas affecting both the right humerus and scapula with focal soft tissue swelling; a CT scan confirmed the existence of an aggressive and invasive soft tissue mass affecting the scapulohumeral joint. Fine needle aspiration results suggested a low-grade synovial sarcoma and therefore a scapulectomy was performed. The biopsy showed spindle to stellated cells immersed in a basophilic and mucinous (myxoid) matrix with mild to moderate anisocytosis, moderate anisokaryosis, some binucleated cells and sporadic multinucleated cells. These findings are consistent with low-grade synovial myxosarcoma, a not well described synovial neoplasm that can mimic other commonly seen joint tumors or even septic arthritis on radiographs. The purpose of this case report is to describe the first reported synovial myxosarcoma affecting the scapulohumeral joint of a small dog.

Seven-Year Follow-Up of Tumors in Young Dogs in the Republic of Serbia

Abstract Although the incidence of neoplasms increases with age, some tumors occur more commonly in younger dogs. Still, little information is available on the occurrence of neoplasms in dogs up to the age of 24 months. This study is a retrospective review of histopathological diagnoses of neoplasia at our laboratory during the last seven years (2017–2023) in two groups of dogs: group up to the age of 12 months and group from 13 to 24 months. In a total of 3704 neoplasms found in different dog tissues, 74 (2.00%) were identified in dogs up to 12 months and 135 (3.64%) in dogs aged 13 to 24 months. Canine cutaneous histiocytoma was the most frequent in both groups:41 cases (54.41%) in dogs up to 12 months, followed by 39 cases (28.89%) in those up to 24 months. In the present analysis of the group up to 12 months, 82.43% of tumors (n = 61) were benign, and 17.57% (n=13) were malignant, followed by 68.15% (n=92) benign neoplasms, and 31.85% (n=43) malignant in dogs aged from 13 to 24 months. French bulldogs and mixed-breed dogs were the most frequently affected. These data provide valuable epidemiological information on neoplasms in young dogs in the Republic of Serbia.

Neuronal Ceroid Lipofuscinosis in a Mixed-Breed Dog with a Splice Site Variant in CLN6.

A 23-month-old neutered male dog of unknown ancestry presented with a history of progressive neurological signs that included anxiety, cognitive impairment, tremors, seizure activity, ataxia, and pronounced visual impairment. The clinical signs were accompanied by global brain atrophy. Due to progression in the severity of disease signs, the dog was euthanized at 26 months of age. An examination of the tissues collected at necropsy revealed dramatic intracellular accumulations of autofluorescent inclusions in the brain, retina, and cardiac muscle. The inclusions were immunopositive for subunit c of mitochondrial ATP synthase, and their ultrastructural appearances were similar to those of lysosomal storage bodies that accumulate in some neuronal ceroid lipofuscinosis (NCL) diseases. The dog also exhibited widespread neuroinflammation. Based on these findings, the dog was deemed likely to have suffered from a form of NCL. A whole genome sequence analysis of the proband's DNA revealed a homozygous C to T substitution that altered the intron 3-exon 4 splice site of CLN6. Other mutations in CLN6 cause NCL diseases in humans and animals, including dogs. The CLN6 protein was undetectable with immunolabeling in the tissues of the proband. Based on the clinical history, fluorescence and electron-microscopy, immunohistochemistry, and molecular genetic findings, the disorder in this dog was classified as an NCL resulting from the absence of the CLN6 protein. Screening the dog's genome for a panel of breed-specific polymorphisms indicated that its ancestry included numerous breeds, with no single breed predominating. This suggests that the CLN6 disease variant is likely to be present in other mixed-breed dogs and at least some ancestral breeds, although it is likely to be rare since other cases have not been reported to date.

Mathematical modeling of biomechanical elastic and hyperelastic properties of the myocardium

BACKGROUND: The study of mechanical properties of biological tissues is extremely informative and is one of the most important areas of biomechanics. Knowledge of these aspects of biological objects based on experimental data can become a source of new medical and technical solutions for the reconstruction of organs and the development of replacement materials. AIM: Passive mechanical properties of isolated myocardium are compared with linear, bilinear, exponential and the most common hyperelastic models (neohookean, Mooney–Rivlin, Ogden, Yeoh, polynomial and Veronda–Westmann). MATERIALS AND METHODS: Literature data on mechanical tests of autopsy material obtained from mongrel dogs were used as initial data. To search for the most advanced calculation algorithms the computer algebra system was used, the Mathcad 15.0 software package and the multifunctional finite element analysis application ANSYS 2022 R2 were used. Direct comparison of models was made based on mathematical statistics. RESULTS: Among the first group of models, the results closest to the experimental data were demonstrated by the exponential model R = 0.9958/0.9984 (in the longitudinal/transverse direction with respect to the myocardial fibers), the lowest accuracy was demonstrated by the linear model R = 0.9813/0.9803. Young’s moduli of linear, bilinear and exponential models and material constants of hyperelastic models are determined. The coefficient of elastic anisotropy of the myocardium, defined as the ratio of the elastic moduli of the linear model measured along and across the direction of the fibers, is equal to 2.18, which is very different from the literature data for the myocardium of the human heart. Deformation along the fibers of the heart muscle is more energy-consuming in the direction along the fibers than in the transverse direction (3.81 and 2.52 mJ/cm3). The most accurate hyperelastic models turned out to be the 2nd order polynomial model R = 0.9971 and the 3rd order Yeoh model R = 0.997. The largest deviations and the lowest correlation coefficient between the experimental and model data were demonstrated by the simple neohookean model R = 0.974 with a single parameter μ. The numerical values of the parameters of hyperelastic models obtained by calculation methods used practically did not differ from each other (≤2.16%). CONCLUSIONS: The study demonstrated the importance of selecting the correct mechanical model for isolated myocardium. The data obtained can be useful in virtual interventions (simulations) for predicting outcomes and supporting clinical decisions, developing replacement materials and structures made of them for reconstructive operations on heart structures.

Эффективность антисептической композиции при использовании спице-стержневых аппаратов наружной фиксации

The purpose of the study is to create and test a device that increases the effectiveness of treatment of open and sutured musculocutaneous wounds during open fractures and immersion osteosynthesis. To improve the healing process of open wounds and reduce possible complications, a group of inventions was developed, united by a common concept. One of the key elements of this group is the new composition. It helps create an aseptic environment for effective wound healing, reliably protects them from possible infections and contamination, and is also successfully used to prevent the development of purulent complications and postoperative osteomyelitis. To prepare it, you need to mix 150 mg of rifampicin, 4 mg of dexamethasone, 2.5 ml of 1 % dioxidine, 15 ml of 95 % ethyl alcohol, bringing the total volume to 100 ml using saline. This composition, used in combination with various external fixation devices, has outstanding antimicrobial, anti-inflammatory and wound healing properties. Another invention is an innovative device with a built-in dosing element, applicators and capillary tubes connected to a manifold, it provides precise and controlled delivery of the composition we have developed to the wound. This eliminates the need for frequent dressings and manual treatment of places where pins and rods penetrate or exit the body, which not only alleviates the patient’s discomfort, but also reduces the risk of developing secondary infections during the postoperative recovery period. In the period from 07/14/2021 to 05/31/2023, 15 mongrel dogs with open comminuted fractures of the right (8 heads) and left tibia (7 heads) were delivered to the veterinary clinic BION (Sevastopol), during the postoperative treatment of which they were tested antiseptic composition and modernized device. The results indicate the reliability and high efficiency of the resulting composition in the treatment and prevention of purulent-inflammatory processes in bones and soft tissues using pin-rod devices for fixation.

Age, sex and breed effect on laboratory parameters in natural Babesia canis infection

We tested the hypothesis that age, breed, and sex are related to hematology, biochemistry, acute phase proteins (APPs), seroreactivity and level of parasitemia in dogs with an acute phase response (APR) due to Babesia canis infection. The study enrolled 61 privately owned dogs that naturally acquired B. canis infection. Groups were formed according to the age: young dogs less than one year, and adult dogs more than one year old. Moreover, the group of males was compared to females and purebred to mixed breed dogs. Seroreactivity was tested with immunofluorescence antibody test, level of parasitemia with real-time polymerase chain reaction (real-time PCR), hematology, and biochemistry with automatic analyzers, serum amyloid A with enzyme-linked immunosorbent assay, fibrinogen with heat precipitation and ceruloplasmin and paraoxonase-1 with manual spectrophotometric methods. For protein separation agarose gel electrophoresis was used. The main changes in the whole population of B. canis-infected dogs were fever, pancytopenia, and change in APPs level. One-third of young, and 96% of adult dogs were seropositive (P < 0.001). The level of parasitemia was higher in the young dogs (P < 0.001). Erythroid lineage parameters (P < 0.01), and leukocytes (P < 0.05) were lower in the young, when compared to the adult dogs. Young dogs had lower total globulins (P < 0.001), β- and γ-globulins (P < 0.001), and higher α-globulins (P = 0.022) than adult dogs. Young dogs had higher concentrations of phosphate (P = 0.003) and cholesterol (P < 0.001) and lower amylase (P = 0.014) and lipase activity (P = 0.020) than adult ones. Male dogs had lower neutrophil count than females (P = 0.035), and purebred dogs had more band neutrophils than mixed breed dogs (P = 0.004). In conclusion, dogs with natural Babesia canis infection at a young age have more severe anemia and APR including leukopenia than adults. Male and purebred dogs might also have more severe APR than females and mix-breeds, as they have more pronounced changes related to the myeloid lineage.

Effects of total intravenous anesthesia using ketamine and propofol combinations on physiological and anesthetic parameters in xylazine-premedicated mixed-breed dogs

The physiological effects of total intravenous anesthesia (TIVA) using three different combinations of ketamine and propofol (“ketofol”) at 1:1 (KP1), 1:2 (KP2) and 1:3 (KP3) in nine xylazine-premedicated adult mixedbred dogs were assessed. The dogs weighing 13.6 ± 2.7 kg were divided into three treatment groups in a repeated crossover experiment. A single bolus injection was used for induction, whereas constant rate infusion (CRI) was utilized for maintenance of anesthesia. All combinations lowered the respiration rates with no variations among the protocols (p=0.197). Within protocols, the rate was significantly lower than the preinduction values at the 30th (p=0.019) and 40th (p=0.041) minutes in KP1 and at the 30th minute (p=0.038) in KP2. The pulse rate was within the normal physiological range, with no differences between protocols (p=0.062); however, within protocols, the rate was significantly lower after the 30th minute in KP1 and after the 15th minute in KP2 postinduction. The temperature was within the normal physiological range but relatively higher in KP1 (p&lt;0.001), with no differences among the protocols during maintenance (p=0.925). The oxygen saturation was generally low and did not differ significantly among the groups (p=0.542). The induction and recovery qualities were rapid and smooth, respectively, in all treatments. In all the treatments, apneustic breathing was the most common side effect. In conclusion, all ketofol ratios can be used in mixedbred dogs; however, the 1:3 (KP3) combination had relatively more stable physiological parameters, fewer side effects and quicker recovery.

Leptospirosis in a mixed breed female dog (Canis familiaris)

The present study aimed to report a clinical case of leptospirosis diagnosed in a mixed breed dog where treatment was chosen without hospitalization. Leptospirosis is an infectious and contagious zoonotic disease caused by the spirochete of the genus Leptospira spp. All pathogenic lineages belong to a single species, Leptospira interrogans. This microorganism can affect both humans and animals, be they domestic, wild or production. Dogs and cats are susceptible, but felines have greater resistance to leptospirosis. Synanthropic rodents in large urban centers or rural areas constitute a major problem, as they end up transmitting it to domestic and stray animals. Animals become infected and end up eliminating spirochetes into the environment through contaminated urine, since leptospirosis has a predilection for the renal tubules. Transmission can be direct through contaminated urine, venereal secretions, placental transfer, bite wounds or ingestion of contaminated tissues. Leptospira can enter the body through intact oral, nasal or ocular mucous membranes, or through skin that is damaged or macerated by moisture. Indirect transmission occurs through contact with contaminated water, soil and food sources. In Brazil, where many locations lack a garbage collection system and sewage disposal is precarious, the tropical climate favors rain during summer, all increasing the role of rodents as vectors of this zoonosis due to flooding. The canine mentioned in this study did not have their vaccination protocol up to date, lived in a place with the presence of rodents, and was diagnosed positive for L. interrogans serovars Bratislava, Pomona and Copenhageni by the microscopic agglutination test (MAT), considered the gold standard. The changes in the blood count were limited to normocytic normochromic anemia and hyperproteinemia. No noteworthy changes were observed in the white blood cell count and, in the biochemical tests, liver and kidney changes consistent with the pathology were evident. The therapeutic protocol chosen was antibiotic therapy and supportive therapy, both carried out at the guardian's home, and the prognosis was favorable. Preventive vaccination measures according to the challenge faced in the environment, rodent control and also public policies regarding basic sanitation are important in preventing in zoonosis.