Mitochondrial COI Gene Research Articles

Genetic Diversity and Population Structure of Eight Cultured Hypophthalmichthys molitrix in Hubei Province, China, Inferred from Mitochondrial COI Gene

Genetic Diversity and Population Structure of Eight Cultured <i>Hypophthalmichthys molitrix</i> in Hubei Province, China, Inferred from Mitochondrial COI Gene

Deep machine learning identified fish flesh using multispectral imaging

Food fraud is widespread in the aquatic food market, hence fast and non-destructive methods of identification of fish flesh are needed. In this study, multispectral imaging (MSI) was used to screen flesh slices from 20 edible fish species commonly found in the sea around Yantai, China, by combining identification based on the mitochondrial COI gene. We found that nCDA images transformed from MSI data showed significant differences in flesh splices of the 20 fish species. We then employed eight models to compare their prediction performances based on the hold-out method with 70% training and 30% test sets. Convolutional neural network (CNN), quadratic discriminant analysis (QDA), support vector machine (SVM), and linear discriminant analysis (LDA) models perform well on cross-validation and test data. CNN and QDA achieved more than 99% accuracy on the test set. By extracting the CNN features for optimization, a very high degree of separation was obtained for all species. Furthermore, based on the Gini index in RF, 11 bands were selected as key classification features for CNN, and an accuracy of 98% was achieved. Our study developed a successful pipeline for employing machine learning models (especially CNN) on MSI identification of fish flesh, and provided a convenient and non-destructive method to determine the marketing of fish flesh in the future.

Genetic structure of Macrobrachium nipponense, an important farmed freshwater shrimp in China, in the Three Gorges Reservoir

Macrobrachium nipponense is a major farmed species freshwater shrimp in China and its genetic diversity is of high value in aquaculture. The construction of the Three Gorges Project has slowed down the water velocity in the Three Gorges Reservoir (TGR) and made the water more clear, which is more favourable for the survival and reproduction of M. nipponense. However, there are lack of studies on the genetic diversity of M. nipponense populations in the TGR. In this study, mitochondrial COI gene sequences were used as molecular marker to analyze the genetic diversity, genetic differentiation and historical demography of eight M. nipponense populations in the TGR. The results showed that the M. nipponense populations in TGR have high genetic diversity. There was some genetic differentiation between the four populations in lower reaches of the TGR and four populations in the upper reaches of the TGR. We also found that there was a significant correlation between genetic distance and geographical distance among the M. nipponense populations in the TGR. The M. nipponense population in the TGR has experienced population reduction in the recent year, which might be related to the ice age movement in the Little Ice Age and human activities. This study provides a theoretical basis for the rational development and protection of M. nipponense resources in the TGR.

First record of the phyllosoma larva of the pygmy locust lobster Scyllarus pygmaeus (Crustacea, Decapoda) in the eastern Mediterranean sea

Abstract Taxonomic intricacies and high interspecific similarity have hampered the identification of scyllarid phyllosoma larvae to the species level. The pygmy locust lobster, Scyllarus pygmaeus, is distributed across the Mediterranean Sea and in the eastern Atlantic; however, its phyllosoma larvae were previously recorded only from the western Mediterranean. We employed DNA barcoding using the mitochondrial COI gene to identify S. pygmaeus phyllosoma collected from the offshore waters of the southeastern Mediterranean Sea and described its morphology. We further discuss the lack of genetic structure in S. pygmaeus with potential implications for species connectivity and conservation.

An integrative taxonomic approach to the Atlantic Hansarsia (formerly Nematoscelis) yields new krill taxa (Crustacea: Euphausiidae)

A recent molecular phylogenetic and biogeographic study on the krill genus Hansarsia revealed undescribed cryptic diversity in the Atlantic. Each of four species analysed encompassed robust molecular clades that were linked to dimorphic males in H. microps, H. atlantica and H. tenella. We tested the robustness and divergence of the observed clades using an integrative approach including (1) three independent species delimitation methods for the mitochondrial COI gene (ASAP, GMYC, bPTP), (2) variability of two nuclear genes (H3 and ITS1) and (3) morphological analysis (MDS and PCA) with a dataset of 22 characters scored for 131 specimens. Both molecular and morphological analyses resulted in at least six distinct clades within the Atlantic Hansarsia. The strongest divergence was revealed between the two clades of H. tenella, one of which we diagnosed as a new species. Two clades of H. megalops also showed significant divergence but in the absence of males, we were reluctant to designate new species. Different clades linked to male forms in H. microps and H. atlantica are suggested as an incipient species. We also hypothesise an unusual trend in the evolution of euphausiids, in which visual recognition enhances tactile interaction during mating. Our results show that analyses of ostensibly well studied groups may yet yield taxonomic surprises. ZooBank: urn:lsid:zoobank.org:pub:AE045636-50EF-450A-B9B3-9231E8B91522

Phylogeography of anabas testudineus bloch, 1792 (pisces: anabantidae) in asia

Anabas testudineus, the climbing perch, is a valuable and well-liked freshwater fish with significant commercial worth. This fish is found in Indonesia and other parts of Asia. Nonetheless, there hasn't been much research done on the genetic relationships across Asian populations of climbing perch. Thus, the aim of this work was to use the mitochondrial COI gene to examine the phylogeography of A. testudineus in freshwater Asia. The population sample was taken from South Aceh, Indonesia. The sample was extracted using a modified C-TAB protocol. In all, 48 sequences from the Genbank and three sequences from South Aceh were included in this investigation. Seven samples came from Indonesia, three from Malaysia, nine from Philippines, seven from Vietnam, eight from Thailand, ten from India, and four from Bangladesh. According to the findings, the sequences formed 17 different haplotypes. For every population, the haplotype diversity (Hd) value is 0.897. The genetic distance measuring the closest populations is 0.0007 between Indonesia and Philippines, whereas Indonesia and Vietnam populations are the furthest apart, at 0.0904. Between 0 and 0.0157 is the genetic distance within the population. Thus, the populations of A. testudineus in Aceh and Indonesia and the Philippines are genetically more similar.

New species and new stages of Anacroneuria (Plecoptera: Perlidae) from Southern Yungas (Argentina and Bolivia)

Austral Yungas cloud forest (Southern Bolivia and NW Argentina) is known for its high diversity, steep landscape and low accessibility. Anacroneuria Klapálek (Plecoptera: Perlidae) species in this ecoregion have been seldom studied. We aim to know its species diversity (based on adult characters) and explore larval characters important for their recognition. We have reared dozens of larvae from different localities and collected adults with light traps. Reared adults and associated larval exuviae were studied, photographed and drawn. We have identified 13 species (based on male aedeagus) and successfully associated larval and adult stages for seven species. In the present paper we describe seven new species: Anacroneuria tafi sp. nov., A. flavinigra sp. nov., A. saican sp. nov., A. triplex sp. nov., A. calilegua sp. nov., A. lutea sp. nov., and A. tupacamaru sp. nov. For six species previously described we present new data and stage descriptions: A. arrazayalensis Orce, A. handlirschi Klapálek, A. reedi (Navás), A. tucuman Stark, A. saltensis Froehlich, and A. chimborazo Stark. Genetic divergence for some of these species was studied using the barcode region of the mitochondrial gene COI. A key to the male adults of all the species of Anacroneuria from Northwestern Argentina is presented.

Genetic Diversity of Kırklareli Honey Bee (Apis mellifera L.) Populations in Thrace Region of Turkey: Identification of Mitochondrial COI and ND5 Gene Regions

The main goal of genetic resource conservation is to keep as much genetic diversity as possible within each species. In this respect, some difficulties in the protection of honey bee gene resources make it necessary to reveal the genetic structures of the subspecies and the genetic relationships between the subspecies. In this study, Kırklareli honey bee populations which were officially registered as an ecotype of Turkey’s honey bee (Apis mellifera L.) gene resources by the Republic of Turkey, Ministry of Agriculture and Forestry, were examined in the COI and ND5 genes of mitochondrial DNA. The restriction fragment length polymorphism (RFLP) together with the polymerase chain reaction (PCR) was used to define Apis mellifera populations. A total of 117 worker bee samples were used which were collected from mostly the Kırklareli province. A newly found single nucleotide polymorphism (SNP), G→A transition in the COI gene region formed a novel NcoI restriction site resulting in a new haplotype. This new haplotype has been abbreviated as haplotype C. As a result of the COI/SspI digestion, the previously reported C haplotype was determined. No restriction was found with the treatment of COI/StyI enzyme. On the other hand, as a result of ND5/AluI restriction, 2 restriction site and previously reported haplotype C was obtained in all of the studied samples. No restriction was screened with ND5/FokI and ND5/HincII enzymes in the whole samples, only a reported uncut B haplotype was observed. Within this study, novel genetic information has been revealed for the Kırklareli honey bee ecotype registered as the Thrace honey bee of Turkey’s honey bee gene resources. Moreover, detailed studies with larger sample sizes should be conducted to characterize the origin and the subspecies of Kırklareli honey bees in detail. It is thought that this study will be useful in the identification and registration of the Kırklareli honey bees to be carried out in the future, and also in the creation of a database.

A new Asian lazy toad of the genus Scutiger Theobald, 1868 (Anura, Megophryidae) from southern Tibet, China.

In this study, a new species named Scutigerluozhaensissp. nov. is described from Luozha, southern Tibet, China. Genetic analysis based on two mitochondrial genes 16S rRNA and COI and the nuclear gene RAG1 revealed that the new species belongs to an independent phylogenetic clade close to S.gongshanensis and S.nyingchiensis and shares no RAG1 haplotype with other species. Morphological comparisons based on examined specimens and literatures indicated that it can be diagnosed from congeners by the following combination of characters: (1) body moderate, male body length 47.0-67.2 mm (n = 13), female body length 49.8-66.2 mm (n = 8); (2) maxillary teeth and budding absent; (3) numerous tiny dense nuptial spines present on dorsal surface of fingers I, II and inner surface of finger III of males in breeding condition with similar size; (4) spine patches on belly of males in breeding condition absent; (5) spines on inner surface of forearm and upper arm of males in breeding condition absent; (6) small patches of black spines present near armpit of males in breeding condition absent; (7) adult males without vocal sac; (8) some large warts and tubercles on dorsum gathered into short skin ridges with several spines present on top; (9) space between upper eyelids wider than upper eyelids; (10) spots or irregular cross bands on limbs absent; (11) webbing between toes rudimentary; (12) coloration of dorsal body olive brown to bronze.

Population genetics of Aedes albopictus in the port cities of Hainan Island and Leizhou Peninsula, China

BackgroundAedes albopictus is an important vector of arboviral diseases, transmitting yellow fever, dengue fever, chikungunya and Zika. Monitoring its population genetic diversity and genetic differentiation has become essential for the control of infectious disease epidemics, especially in the functional areas of ports of entry. Population genetic monitoring of Ae. albopictus in the port area can help in the monitoring of port mosquito invasions and establishing port sanitary and quarantine measures to prevent the introduction and transmission of vector-borne diseases. MethodsSeventeen populations of Ae. albopictus were collected from five port cities on Hainan Island and the Leizhou Peninsula, 8 populations were collected from port areas, 4 from urban areas and 5 from rural areas. Nine microsatellite loci and the mitochondrial COI gene were used to study the population genetic diversity, population genetic structure and interpopulation gene flow of Ae. albopictus. ResultsThe nine microsatellite loci used were highly polymorphic, with an average PIC value of 0.768. The UPGMA genetic tree, STRUCTURE barplot and PCoA analyses showed that the 17 Ae. albopictus populations could be divided into three genetic groups. All 17 populations showed high haplotype diversity (Hd = 0.8069–0.9678) and formed 133 distinct haplotypes. These haplotypes can be divided into four genetic clades, but they are not associated with the geographical distribution of Ae. albopictus. Fst and Nm showed strong gene flow and little differentiation among populations. ConclusionAe. albopictus in port areas are not significantly different from urban and rural populations due to strong gene flow, which prevents differentiation and increases the genetic diversity of the populations. High genetic diversity facilitates mosquito adaptation to complex environmental changes, which is a challenge for vector-borne disease control in port areas.

The Grasshopper Paradigm in damselflies: evidence for phalanx-like postglacial recolonization of Europe from a Balkan refugium in Platycnemis pennipes Pallas (Odonata: Zygoptera: Platycnemidae)

We explore haplotype diversity, phylogeography and phylogenetic relationships of the damselfly Platycnemis pennipes in Europe based on 618 bp DNA from the mitochondrial gene COI. A haplotype network analysis shows that the species is divided into two haplotype groups. One is restricted to the Italian Peninsula, while the other is found from the Black Sea region across eastern and central Europe to Scandinavia, England, and southwestern France. This pattern is recovered in a Bayesian phylogenetic analysis. Genetic distance (K2P) between the two groups is approximately 1.5%, while within-group variation is an order of magnitude lower. An analysis of the molecular variance (AMOVA) shows that variation between the two groups account for more than 96% of the total variation within the dataset, adding to the evidence that they have been isolated for a considerable amount of time. The pattern we find is similar to the so-called Grasshopper Paradigm in European phylogeography, where a species has recolonized Europe after the last glaciation from a glacial refugium in the southeast, while other refugial populations in the Iberian and Italian peninsulas have remained isolated to this day. In P. pennipes there is only an isolated refugial population in Italy as the species does not have current populations in the Iberian Peninsula. By comparing the genetic distance between the two groups to a previously published divergence time analysis of European Odonata we estimate that they have likely been isolated since the onset of the Saale Glaciation ca 400 ky ago.

Pleistocene climatic oscillations influenced the emergence of geographically widespread and restricted genetic lineages in an Andean grasshopper species group

Abstract The grasshopper Trimerotropis pallidipennis species complex is distributed across arid and semi-arid environments from North to South America, reaching high altitudes along the Andes Mountain range. Currently, there are four valid species of Trimerotropis in South America. However, some species were found to comprise several genetic lineages. Here, we describe the genetic structure, lineage diversification, and environmental requirements within the Trimerotropis species complex in South America based on sequences from the mitochondrial COI gene, 4048 single nucleotide polymorphisms, and ecological niche modelling. The analyses revealed the existence of at least five lineages: two with a restricted geographical distribution and three with a very wide one. The results provide evidence that glacial cycles promoted not only the presence of refuge areas but also the isolation of widely distributed lineages on the eastern and western sides of the southern Andes, potentially driving the emergence of the Trimerotropis sp. lineage, which can tolerate more temperate habitats. Our results provide evidence for the effects of Pleistocene climatic changes on the diversification history of a South American grasshopper species complex.

Mito‐nuclear discordance and phylogeography of the surf clam Mesodesma donacium along the Southeast Pacific coast

AbstractMesodesma donacium is a surf clam endemic to the southeast Pacific coast, and it is an important resource species of the sandy beach artisanal fishery in Peru and Chile. Over time, the species has shown high variability in population dynamics (presence and abundance), which has been attributed to overfishing and environmental events. In this study, we assess the phylogeography of seven natural beds of M. donacium (17 to 42° S), to reveal the geographic distribution of the genetic diversity through the analysis of 278 sequences of the mitochondrial gene COI. Sequences of the nuclear genes 18S and 28S were used to evaluate the divergence of COI haplogroups. Two divergent parapatric mitochondrial haplogroups were found, which lacked divergence at nuclear markers (18S and 28S); this mito‐nuclear discordance allows inferring that there is no reproductive isolation of mitochondrial haplogroups. The North haplogroup is the only one present at the northernmost site, while only the South haplogroup was present in Cucao in the south. Between 28° and 32° S, coincident with a coastal biogeographic break at 30° S, both haplogroups are in sympatry. Haplogroups differed in their genetic structure, with the North haplogroup representing a single, highly diverse population and the South haplogroup with genetic differentiation and more restricted genetic diversity and gene flow. The divergence in mitochondrial haplogroups without nuclear divergence suggests a past scenario of geographic isolation in the northern and southern areas, without developing reproductive isolation, followed by secondary contact. Given the phylogeography, genetic information should be considered in restocking and management activities.

The molecular phylogeny of cirrate octopods (Cephalopoda: Octopoda: Cirrata) using COI and 16S sequences

Contrasting accounts of taxonomic relationships between finned octopod (Octopoda: Cirrata) taxa complicate species identification and generate nomenclatural instability. A comprehensive analysis based on both 16S and COI mitochondrial gene phylogenies is presented, using all currently accepted genera and approximately 27 putative species (including type species for all genera excepting Grimpoteuthis). The goal of this is to stabilise the concepts of genera and families and identify areas needing further systematic research. Four well supported clades are consistent with families Cirroteuthidae, Cirroctopodidae, Grimpoteuthididae, and Opisthoteuthidae across both 16S and COI phylogenies. Family Stauroteuthidae resolves as a sister clade to Cirroteuthidae in some analyses of the COI gene. Combined molecular and morphological similarities suggest that the Cirrata comprises two superfamily level clades, for which Cirroteuthoidea (for Cirroteuthidae and Stauroteuthidae) and Opisthoteuthoidea (for Cirroctopodidae, Grimpoteuthididae, and Opisthoteuthidae) are proposed. The paraphyletic Cirrothauma (Cirroteuthidae) contains at least three species, while within the Grimpoteuthididae, Cryptoteuthis and Luteuthis form clades within a paraphyletic Grimpoteuthis clade, indicating a need for further taxonomic assessment. Intra-clade structure within Opisthoteuthis is better resolved with COI than 16S data. At least one potentially undescribed Opisthoteuthis occurs in the northwestern Pacific, and northeastern Pacific specimens are tentatively referred to O. bruuni, representing a significant range expansion. Lastly, using the dates of fossil stem-Octobrachians and fossil cirrates for approximate calibration, a timetree estimated from COI gene data suggests that these cirrate families and most cirrate genera arose within the Late Cretaceous, possibly as part of the Mesozoic marine revolution, between 124−62 million years ago.

Morphological and molecular identification of Callithrix sp. hybrids

The common marmoset (Callithrix jacchus) and black-tufted marmoset (Callithrix penicillata) are endemic species of Brazil. Currently, both species are distributed in different regions of the country, being the main cause the illegal trafficking. The introduction of exotic animals is the second biggest cause of biodiversity loss globally and can lead to hybridization. In Bauru, São Paulo (SP), there is a record of both species and animals showing intermediate patterns between them. Thus, this work aimed to identify individuals of the genus Callithrix present in the Municipal Botanical Garden of Bauru (MBGB) as either pure or hybrids, through morphological and molecular markers. Ten individuals were sampled, 4 of which were pure species for control and six free-living, which were photographed, morphometrically measured, and from which the fragments of the mitochondrial genes COI and D-loop region were amplified. As a result, all animals showed intermediate patterns between both species. After analyzing the sequences obtained for D-loop and obtaining a high haplotypic diversity (h), low nucleotide diversity (π), Tajima D and Fu’s Fs statistic, it is possible to infer that the animals in the area continue receiving gene flow and the population began from a founder effect. Morphological and molecular data indicate that the individuals sampled are hybrids. From the data generated by this research, it is possible to plan the insertion of management of these animals in the Conservation Unit (UC), to avoid population growth and its outcomes.

Phylogenomics and systematics of botryllid ascidians, and implications for the evolution of allorecognition

Allorecognition, the ability of an organism to distinguish kin from non-kin, or self from non-self, has been studied extensively in a group of invertebrate chordates, the colonial ascidians called botryllids (Subphylum Tunicata, Class Ascidiacea, Family Styelidae). When two conspecific botryllid colonies come in contact, there are two potential outcomes to an allorecognition reaction: fusion or rejection. The rejection outcome of allorecognition varies by species, and has been classified by type (referred to as R-Type). R-Type is defined according to how far the fusion process progresses before the rejection begins, since the rejection reaction appears as an interference of the fusion process. Here, we map the evolution of R-Types onto an extended and robust phylogeny of the botryllids. In this study, we have reconstructed the largest phylogenomic tree of botryllids, including 97 samples and more than 40 different species, and mapped on it nine of the 13 species for which the R-Type is known. Based on the R-Type known in a single outgroup species (Symplegma reptans), we infer that at least R-Type B and E-like could be ancestral to the Botrylloides/Botryllus group. However, the application of ancestral character state reconstructions does not provide conclusive results since several clades show more than one equiparsimonious R-Type state. Notably, all R-Type A species are clustered together and certainly evolved later than other R-Types. Our phylogenomic tree has been built on 177 nuclear loci and nearly all clades are well supported. Moreover, our phylogenetic analyses also take into account the results of species delimitation analyses based on the mitochondrial COI gene and of careful morphological analyses of the samples. The implementation of this integrated taxonomic approach, combining morphological as well as nuclear and mitochondrial data, has allowed the description of six new species, and the identification of a number of putative unnamed taxa. Thus, our results also demonstrate the existence of an unexplored hidden diversity within botryllids.

Molecular analysis of the blood cockle, Tegillarca granosa (Linnaeus 1758) from Indonesia

The blood cockle, Tegillarca granosa, is one of the key member of shellfish communities that are ecologically and economically important specially in Indonesia. We collected samples from four sites in Indonesia: Banyuwangi, Bali Island, Maros and Buru Island. Nucleotide sequence analysis was performed on PCR-amplified mitochondrial DNA COI gene. In total, 556-558 base pair nucleotide sequences were obtained from 12 individuals. Molecular analyses revealed samples belonging to the T. granosa species. Phylogenetic analyses were carried out by comparing the gene sequences of T. granosa haplotypes in Indonesia with gene sequences of the same base pair length and with the of Anadara tuberculosa which was used as an outgroup. The phylogenetic trees were consistent and indicated in the two sub-clades. The presence of T. granosa and the phylogenetic status of the T. granosa Indonesian haplotype were reported. Clarifying the phylogenetic status of ecologically important species provides basic information studies for possible future conservation and control programs.

Filter no more: A modified plankton sampler for rapid in‐water eDNA capture

Abstract The combination of an efficient sampling method and high‐throughput analysis of environmental DNA (eDNA) can be a powerful approach for characterising biodiversity across aquatic ecosystems. Plankton net tows are one of the oldest, simplest, and least expensive methods for seston and eDNA collection, but require laborious filtration steps which often lead to clogging and/or the introduction of contaminants. In this study, we used a cruising speed net (CSN) device enabling the collection of seston‐derived eDNA at 5 knots speed combined with a novel modified cod‐end with 20 μm nylon mesh inserts enabling eDNA capture while towing. We compared the performance of the CSN sampling protocol with the original conventional filtration of water sample versus the modified cod‐end. Samples were collected in parallel horizontal tows along New Zealand's North‐Eastern coastline. Concentrated water was filtered on conventional 5 μm cellulose acetate membranes, while the 20 μm nylon mesh inserts were immediately isolated post‐towing. Metabarcoding of bacterial 16S rRNA, eukaryotic nuclear 18S rRNA and mitochondrial COI genes, revealed no significant difference in alpha diversity between filtration techniques. In terms of community composition, a clear and significant shift could be observed between sampling sites and environments. Significant differences could be detected between filtration methods for 16S and COI markers, likely driven by fine‐scale differences at more turbid sheltered sites. Nonetheless, each technique could detect shifts in communities between sites and environments with similar sensitivity. Our results demonstrate the promising potential of the modified cod‐end to enable practical and cost‐effective isolation of eDNA‐derived biodiversity data from any vessel types (at ≤5 knots) across a large range of aquatic ecosystems and biogeographic scales.

Revision of the Peruvian tarantula Homoeomma peruvianum (Chamberlin, 1916): description of a new genus with eleven new species and insights to the evolution of montane tarantulas (Araneae: Theraphosidae: Theraphosinae)

ABSTRACT The Peruvian tarantula Homoeomma peruvianum (Chamberlin, 1916) is revised. As the type specimens do not fit the diagnostic characters of Homoeomma Ausserer, 1871, especially because of the different palpal bulb morphology in males and the shape of spermathecae in females, H. peruvianum (Chamberlin, 1916) was transferred into the newly established genus Urupelma gen. n., which is herein described, diagnosed and illustrated. Eleven new species of Urupelma gen. n. (U. sanctitheresae sp. n., U. sanctimariae sp. n., U. awanqay sp. n., U. ashaninka sp. n., U. atarraz sp. n., U. megantonianum sp. n., U. machiguenga sp. n., U. pampas sp. n., U. johannae sp. n., U. veronicae sp. n., and U. dianae sp. n.) are herein described, diagnosed and illustrated. The monophyly of Urupelma gen. n. is supported by a molecular phylogenetic analysis focused on the tarantulas with urticating setae of type III and III+IV and based on three genes: the nuclear 18S and 28S, and the mitochondrial COI gene. Apart from the support of the new genus and some existing valid genera, the molecular phylogeny also sheds light on the dynamic evolutionary history behind the adaption to high montane environments in tarantulas.

First discovery of the ant genus Eburopone Borowiec, 2016 (Hymenoptera, Formicidae, Dorylinae) in the Oriental realm, with description of a new species from Vietnam.

The doryline ant genus Eburopone Borowiec, 2016 currently contains only one valid species, E.wroughtoni (Forel, 1910) from southern Africa, with a considerable number of undescribed species awaiting formal description in the Afrotropical and Malagasy regions. In the present paper, Eburoponeeasoanasp. nov. is described based on workers and dealate queens from a colony series collected in an evergreen forest on the Dak Lak Plateau of Vietnam (Ea So Nature Reserve, Dak Lak Province). The worker of the new species is morphologically clearly distinguished from E.wroughtoni by the combination of following characteristics: i) frontal line distinct, extending a little beyond mid-length of cranium; ii) anterior (frontoclypeal) margins of torulo-posttorular complex not forming conspicuous lobes protruding over anterior clypeal margin in full-face view; iii) mandibles when closed in full-face view forming only a little space between anterior clypeal margin and mandibles; iv) promesonotal suture faint and inconspicuous; v) abdominal segment III in dorsal view distinctly wider than long, with lateral margins only feebly convex. This represents the first discovery of the genus Eburopone in the Oriental realm, revealing the disjunct distribution of the genus. A partial sequence of the mitochondrial COI gene (658 bp) is provided as a DNA barcode for the new species. A worker-based key to the doryline genera of the Oriental realm is also provided.