Damage Mitigation Research Articles

Enhanced Laser Damage Threshold in Optically Addressable Light Valves via Aluminum Nitride Photoconductors

AbstractOptically addressable light valves (OALVs) are specialized optical components utilized for spatial beam shaping in various laser‐based applications, including optics damage mitigation, and enhanced functionality in diode‐based additive manufacturing requiring high intensities. Current state‐of‐the‐art OALVs employ photoconductors such as Bismuth Silicon Oxide (BSO) or Bismuth Germanium Oxide (BGO), which suffer from limited laser‐induced damage thresholds (LiDT) and inadequate thermal conductivities, thus restricting their use in high peak and average power applications. Aluminum nitride (AlN), an emerging ultra‐wide band gap (UWBG) III–V semiconductor, offers promising optoelectronic properties and superior thermal conductivity (>300 Wm−1K−1 at 298° K, compared to BSO's 3.29 Wm−1K−1). In this study, the first AlN‐based OALVs are designed, fabricated, and experimentally demonstrated using commercially available single‐crystal AlN substrates. These AlN‐based OALVs have shown clear superiority over BSO and BGO‐based devices. Design considerations for OALVs incorporating UWBG photoconductors are discussed, and the photoresponsivity from defect‐mediated sub‐bandgap absorption in AlN crystals is verified as sufficient for OALVs operating under high light fluences. The optimum driving voltage for the AlN‐based OALV is determined to be ≈ 45 Vpp at 100 Hz, achieving a transmittance of 91.3%, an extinction ratio (ER) of more than 100, and a 51:1 image contrast.

Primary and "Pre-Primary" Aldosteronism in Resistant Hypertension: A Practical, Pragmatic, and Prudent Approach in Resource-Limited Milieu.

Introduction Primary aldosteronism (PA), once considered rare, is now recognized as the most common cause of secondary hypertension, accounting for almost a quarter of resistant hypertension (RH) cases. Despite this, PA remains underdiagnosed, with an extremely low percentage of RH patients undergoing screening. Methods In a specialty diabetes-endocrinology clinic, the aldosterone:renin ratio (ARR) was assessed in 115 consecutive RH patients (ages 21-93 years; 47% male; 87% with type 2 diabetes). Fasting blood samples were drawn in a standing position after 30 minutes of walking. Adrenal imaging (CT/MRI) was performed for those with an ARR >20. Results ARR values ranged from 0.4 to 227 (ARR <10 (35%); 11-20 (19%), 21-40 (25%), and >40 (21%)), with corresponding stepwise decreasing plasma renin activity (PRA) (P= 1E-6) and increasing serum aldosterone (SA) (P= 8E-7). Increasing ARR tended to be associated with an increase in serum creatinine (R= 0.23; P= 0.03) and a decrease in estimated glomerular filtration rate (eGFR) (R= -0.24; P= 0.02) and an increase in urine albumin: creatinine ratio. The ARR> 40 group displayed the highest serum creatinine, lowest eGFR, higher urine albumin: creatinine ratio, highest serum sodium, lowest serum potassium, and highest (44%) abnormal adrenal imaging (bilateral hyperplasia diffuse/nodular; solitary adenoma), reflecting a later stage of the pathological spectrum. PA treatment with mineralocorticoid receptor antagonists (MRAs) had a salutary effect. Conclusions Our observations further reinforce that PA is not a binary condition, but exists as a spectrum disorder responsive to MRAs, even in patients with mildly elevated or normal aldosterone levels. Early disease detection/recognition ("renin-independent aldosterone production") can be facilitated by marking "pre-primary" aldosteronism (ARR 11-20), followed by monitoring progression (periodic rescreening) and optimizing treatment, with hopeful mitigation of end-organ damage in RH.

Protein kinase D1 mitigation against etoposide induced DNA damage in prostate cancer is associated with increased α-Catenin.

The E-cadherin, α- and β-Catenin interaction at the cell adherens junction plays a key role in cell adhesion; alteration in the expression and function of these genes are associated with disease progression in several solid tumors including prostate cancer. The membranous β-Catenin is dynamically linked to the cellular cytoskeleton through interaction with α-Catenin at amino acid positions threonine 120 (T120) to 151 of β-Catenin. Nuclear presence of α-Catenin modulates the sensitivity of cells to DNA damage. The objective of this study is to determine the role of α-Catenin and protein kinase D1 (PrKD1) in DNA damage response. Prostate cancer cells; LNCaP, LNCaP (Sh-PrKD1; silenced PrKD1), C4-2 and C4-2 PrKD1 were used for various sets of experiments to determine the role of DNA damage in PrKD1 overexpression and silencing cells. These cells were treated with compound-10 (100 nM) and Etoposide (30 µM), total cell lysates, cytosolic and nuclear fractions were prepared to observe various protein expressions. We performed single cell gel electrophoresis (COMET assay) to determine the etoposide induce DNA damage in C4-2 and C4-2 PrKD1 cells. The animal experiments were carried out to determine the tolerability of compound-10 by mice and generate preliminary data on efficacy of compound-10 in modulating the α-Catenin and PrKD1 expressions in inhibiting tumor progression. PrKD1, a novel serine threonine kinase, phosphorylates β-Catenin T120. In silico analysis, confirmed that T120 phosphorylation alters β- to α-Catenin binding. Forced expression of PrKD1 in prostate cancer cells increased β- and α-Catenin protein levels associated with reduced etoposide induced DNA damage. Downregulation of α-Catenin abrogates the PrKD1 mitigation of DNA damage. The in vitro results were corroborated in vivo using mouse prostate cancer patient derived xenograft model by inhibition of PrKD1 kinase activity with compound-10, a selective PrKD inhibitor, demonstrating decreased total β- and α-Catenin protein levels, and β-Catenin T120 phosphorylation. Alteration in DNA damage response pathways play major role in prostate cancer progression. The study identifies a novel mechanism of α-Catenin dependent DNA damage mitigation role for PrKD1 in prostate cancer.

Revolutionizing Cybersecurity with AI: Predictive Threat Intelligence and Automated Response Systems

The sophistication and breadth of cyber threats are continuously expanding, making it more difficult for traditional security measures to keep up. Artificial intelligence is revolutionizing cybersecurity by equipping businesses to proactively counter threats with automated reaction systems and predictive threat intelligence. Data analytics, behavioral analysis, and machine learning enable AI-powered systems to anticipate cyber assaults, enabling more efficient and rapid threat detection. By automating reaction mechanisms and mitigating threats in real-time, AI systems can minimize human error and maximize damage mitigation. AI techniques, such as anomaly detection, predictive modeling, and real-time threat analysis; data privacy, ethics, and the risks of hostile attacks are among the subjects covered, as are the benefits and drawbacks of utilizing AI in cybersecurity. This article provides the framework for future intelligent, automated cyber defense methods and illustrates how AI may alter cybersecurity using real-life examples and case studies.

Implementación de Disipador de Energía, TADAS

El Salvador is a country with high seismic demand, so the design and construction processes of buildings today areaimed at reducing the vulnerability caused by earthquakes, as part of the philosophy of damage mitigation and correct structural performance, there are Advances in detailing and construction techniques, so the use of deformation. energy dissipation systems is a safe way to prevent damage at a reasonable economic cost compared to the use of other more complex and sophisticated technologies. In El Salvador, the implementation of the TADAS (Triangular plate Added Damping And Stiffness) energy dissipation system has been implemented in a pioneering way and on a small scale, in the case of a two-story building, structured with Steel frames with ordinary detailing. The first level corresponds to parking, so the TADAS devices have only been installed in a longitudinal direction, while the second level corresponds to various uses of meeting people in meeting rooms and office work, so architectural issue the existence of windows with a view not interrupted by structural elements turned out to be a condition for not installing TADAS on that level. The final result meets the requirements of the ASCE-7-16 standard in deformation control, and prevention of structural damage due to the ductility that TADAS provides to the global structure. All the structural elements satisfy resistance and service requirements given by AISC-ANSI-36016. ILIA: Investigaciones Latinoamericanas en Ingeniería y Arquitectura, No. 01, 2024: 103-107.

Synergistic therapeutic effect of Guggul gum resin on antidiabetic activity of saxagliptin

Type 2 Diabetes Mellitus is an endocrine metabolic disorder leading to impaired glucose utilization. This disease is managed by both commercial drugs and herbal products eliciting various interactions. The evaluation of one such interaction between saxagliptin (SAXA, substrate of CYP3A4), and Guggul extract (GE) from Commiphora wightii is done in this present study. The concomitant administration of the SAXA with GE had restored the levels of otherwise increased blood glucose and biochemical parameters and otherwise decreased total glycogen and oxidative stress markers more effectively as compared to the solely consumed SAXA or GE. These results were corroborated by histopathological evaluation where the combination treatment showed better mitigation of hepatic, renal and pancreatic tissue damage that occurred due to induced diabetes. Furthermore, CYP3A11 mRNA (murine homolog to human CYP3A4) expression levels were observed to be reduced to non-diabetic levels in combination therapy which was otherwise unachievable. The molecular docking studies predicted improved interaction between CYP3A4 and bioactive content of GE, i.e., guggulsterone E-Z, possessing binding energy of -9.96 kcal/mol along with SAXA, confirming the co-existence of both the ligands at different binding sites of the metabolizing enzyme. The synergistic interactions of GE and SAXA helped in better restoration of tissue damage and increased CYP3A11 mRNA expression levels caused by induced diabetes.

Weaving the Web to achieve the SDGs: the presence of women in the boardrooms as a key driver

Purpose Due to the importance of mitigating the issues that afflict society and natural habitats on a global scale, the United Nations has established 17 key goals to directly and effectively mitigate problems such as extreme poverty, lack of access to quality educational environments or the loss of life in underwater and terrestrial ecosystems, which need to be addressed from multiple parties, particularly from the business network. In this context, the purpose of this paper is to analyse the potential impact of female participation in boardrooms on the disclosure of corporate information regarding the integration of the Sustainable Development Goals (SDGs) into business strategy. Design/methodology/approach The research hypotheses were developed on the basis of a multi-theoretical perspective, which included agency theory, upper echelon theory, resources dependency theory and critical mass theory. The hypotheses were tested using a regression model for a sample of 8,903 companies for the period 2019–2022. Findings The results highlight the importance of an increase in female boardroom representation as a key driver in the achieving of the SDGs with favourable effects on environmental and social issues, being robust to changes in methodological specifications. Research limitations/implications This research provides valuable evidence for the analysis of gender issues in the business field; however, it has some limitations that can be addressed by academics. In this sense, this research solely examined the impact of chief executive officer (CEO) duality while overlooking the potential for its influence to be amplified by a distinctive configuration of the board, such as the involvement of a former CEO, a greater or lesser degree of participation of executive directors in the boardroom or the fact that the CEO is a woman. These factors could either reinforce or obstruct the benefits of female directors in the accomplishment of the SDGs. Practical implications From a practical standpoint, this research illustrates the advantages of the active involvement of women in the boardroom. Their participation has resulted in notable modifications to corporate strategies, with a focus on aligning business actions with the attainment of the SDGs and related transparency. This facilitates a more comprehensive understanding of the business interest in conserving natural habitats and eradicating social inequalities. Social implications From a societal perspective, the findings illustrate the significance of actively promoting the involvement of women in organisational leadership roles. The inclusion of women's perspectives can enhance the planning of business processes, with far-reaching implications for stakeholders. Therefore, it is vital to guarantee opportunities for women, such as access to higher education and gainful employment, particularly in nations where there are considerable constraints to the intellectual and economic advancement of girls and women. Originality/value The findings contribute to the growing body of knowledge on the optimal configuration of the boardroom, especially the gendered boardroom (i.e. García-Sánchez et al., 2023b; Liao et al., 2015), and its ability to address today's global challenges. Furthermore, the findings indicate the necessity of establishing pathways for women to reach positions of influence within the organisational hierarchy, thereby facilitating beneficial societal changes. These changes can include the mitigation of environmental damage and the rectification of historical social inequalities.

Fatigue damage analysis and mitigation for steel structures using UHM-CFRP prestressed technique

Fatigue stresses in steel bridges can lead to crack propagation, gradually decreasing the structural stability of the bridge. Conventional strengthening techniques for structures have shown limited effectiveness in preventing crack growth. The objective of this research is to enhance the fatigue life of cracked steel beams through the application of ultra-high modulus carbon fiber reinforced polymer (UHM-CFRP) plates. Two sets of tests, including quasi-static testing and fatigue testing, were conducted on both cracked and uncracked steel beams. All beams were tested under identical loading conditions, with a total of five beams examined. Control beams were used to compare fatigue life under different loading ranges. One beam was reinforced with UHM-CFRP plates, another utilized prestressed UHM-CFRP plates, and another employed UHM-CFRP plates with end clamps. The experimental study revealed a significant 8 times increase in fatigue lives for cracked steel beams repaired using the UHM-CFRP compared to the control beam. Additionally, the (10 %) prestressed UHM-CFRP plate not only doubled the fatigue life of identical beams compared to UHM-CFRP plates but also significantly reduced residual deflection during fatigue crack growth, highlighting its efficacy in fatigue resistance.

Bacteriophage P2-71: a promising therapeutic against multidrug-resistant Proteus mirabilis in urinary tract infections.

Proteus mirabilis is a Gram-negative, rod-shaped bacterium widely found in natural environments. It is known for causing a range of severe illnesses in mammals, particularly urinary tract infections (UTIs). This study evaluates the therapeutic efficacy of phage P2-71 against Proteus mirabilis in vivo and in vitro environments. The in vitro therapeutic potential of bacteriophage P2-71 was assessed through the ability of phage to kill Proteus mirabilis by using a plate counting assay, and biofilm inhibition and biofilm lysis assays using a microtitre plate method. Additionally, an in vivo UTI model in C57BL/6Jmice was developed via urethral inoculation of the bacterium. Phage therapy was administered through urethral injection over a period of 5 days. Therapeutic outcomes were measured by analyzing bacterial load, phage titer, inflammatory markers, and histopathological changes in the urine, urogenital tissues, and spleen. In vitro, bacteriophage P2-71 achieved significant reductions in P. mirabilis concentrations, with log reductions of 1.537 and 0.7009 CFU/mL in laboratory and urine environments, respectively (p < 0.001). The phage also decreased biofilm formation by 34-49% and lysed 15-25% of mature biofilms at various multiplicities of infection (MOIs) (p < 0.001). In vivo, phage treatment significantly lowered bacterial concentrations in the urine on Days 1 and 3 (p < 0.0001), achieving a maximum reduction of 4.602 log₁₀ CFU/mL; however, its effectiveness diminished by Day 5 (p > 0.05). Concurrently, phage titers decreased over time. Importantly, phage treatment notably reduced bacterial load in the bladder, kidneys, and spleen (p < 0.001). Inflammatory markers such as IL-6, IL-1β, and TNF-α were significantly lower in the treatment group, especially in the bladder (p < 0.0001), indicating an effective reduction in inflammation. Histopathological analysis showed significant mitigation of tissue damage. The results demonstrated that bacteriophage P2-71 is a promising alternative therapy for UTIs caused by MDR Proteus mirabilis. This bacteriophage therapy offers a viable strategy for managing infections where traditional antimicrobials fail, highlighting its potential in clinical applications.

Earthquake damage mitigation methods for buried pipelines under compressive loads: A case study of the Thames water pipeline

Promoting tensile failure by providing a proper orientation angle between the pipe axis and the fault line is the main seismic design philosophy for buried steel pipelines. However, most of the severe damage and failures experienced by pipelines are mainly due to negative crossing angle and thus compressive loads acting along the pipeline. This paper investigates different earthquake damage mitigation methods such as Carbon Fiber Reinforced Polymer (CFRP) wrapped pipes, Steel Pipes for Fault Crossing (SPF), and corrugated pipes for buried steel pipelines which are mainly subjected to compressive loads. Therefore, the Thames water transmission pipeline, which is a well-known case study, that suffered major and minor damage due to compressive forces in the 1999 Kocaeli earthquake, is considered to simulate and compare the earthquake damage mitigation capabilities of these countermeasures. The numerical studies are performed by using a three-dimensional nonlinear finite element model. The results show that the use of CFRP composites in buried pipelines, regardless of their thickness, wrapping length, or layer orientation, does not have the expected damage reduction effect, but does increase the effective length between major wrinkles or change the type of pipe failure. On the other hand, SPFs and corrugated pipes are more effective in earthquake damage reduction due to their high axial and rotational capabilities.

Experimental study on fatigue damage mitigation in welded beam-to-column connections with passive vibration control

To investigate the effectiveness of a viscous fluid damper (VFD) in mitigating fatigue damage in welded beam-to-column connections, six specimens were manufactured with the full penetration groove weld process. These specimens were divided into three groups: S1, S2, and S3, each containing two specimens. One specimen in each group was equipped with VFD, while the other was not. All specimens underwent the same elastic cyclic loading stage, but a different plastic cyclic loading stage for each group. The study results indicate that the presence of VFD can significantly enhance the load-bearing capacity of the welded beam-to-column connection by 10%–15% when subjected to the same displacement amplitude at the beam end. The primary cause of failure in the welded beam-to-column connection is the fatigue damage of the weld toe on the beam flange, which requires more attention during the welding process. Additionally, the VFD can effectively reduce stress concentration at the weld seam area, leading to a maximum of 50% decrease in the maximum stress near the weld toe of the beam flange center, as observed in our study.

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing

Air-water interface (AWI) interactions during cryo-electron microscopy (cryo-EM) sample preparation cause significant sample loss, hindering structural biology research. Organisms like nematodes and tardigrades produce Late Embryogenesis Abundant (LEA) proteins to withstand desiccation stress. Here we show that these LEA proteins, when used as additives during plunge freezing, effectively mitigate AWI damage to fragile multi-subunit molecular samples. The resulting high-resolution cryo-EM maps are comparable to or better than those obtained using existing AWI damage mitigation methods. Cryogenic electron tomography reveals that particles are localized at specific interfaces, suggesting LEA proteins form a barrier at the AWI. This interaction may explain the observed sample-dependent preferred orientation of particles. LEA proteins offer a simple, cost-effective, and adaptable approach for cryo-EM structural biologists to overcome AWI-related sample damage, potentially revitalizing challenging projects and advancing the field of structural biology.

Damage mitigation strategies for progressive collapse of modular steel buildings following sudden corner module removal

AbstractThis study evaluates gravity‐induced progressive collapse performance and damage mitigation strategies in modular steel buildings (MSBs) A typical three‐dimensional six‐story structure was modeled and analyzed using the finite element method, focusing on the instantaneous removal of a corner module. The study includes a parametric evaluation of the influence of beam moment capacity on progressive collapse and investigates the effect of using concrete as an infill for square hollow section columns. Additionally, the effectiveness of short K‐shaped braces in limiting gravity‐induced collapse was assessed. A response/damage index (DI) was proposed to quantify the overall structural response. The findings indicate that semirigid beam‐column connections outperform fully rigid connections. Both concrete‐filled columns and K‐shaped braces mitigate structural damage, with K‐shaped braces proving more effective in preventing collapse. Although increasing the beam moment‐resisting capacity reduced the DI, improvements were marginal beyond 50% of the fully rigid connection capacity. Higher beam moment capacities reduced local vertical displacement at the corner joint but led to undesirable lateral deformations. Reducing beam rigidity/capacity by 25% mitigated global structural deformations. The optimal beam moment capacity was identified as 75% of the total section capacity when using concrete‐filled columns. K‐shaped braces applied at the column midpoint entirely prevented progressive gravity‐induced collapse and no lateral deformations were observed in braced structures with fully rigid beam‐column connections, unlike unbraced structures. Concrete‐filled columns offer a cost‐effective solution for preliminary production of MSBs, while K‐shaped braces are suitable for retrofitting existing buildings. Further research is necessary to address other parameters influencing the mitigation of progressive collapse in MSBs.

Effect of atmospheric response induced by preceding typhoon on movement of subsequent typhoon over Northwestern Pacific

This study investigates the atmospheric interactions between two closely located typhoons in 2019. Typhoons in the Western Pacific significantly impact Eastern and Southeastern Asian countries, leading to various damages. As global warming is expected to increase typhoon intensity, accurate track forecasting becomes crucial for coastal disaster management. Despite the existing knowledge about the influence of typhoon activities on the atmospheric background, limited research addresses the atmospheric response between two typhoons. The study focuses on the cases of LEKIMA and KROSA, occurring simultaneously in 2019, and utilizes the Weather Research and Forecasting (WRF) model for simulations. The experimental setup involves comparing two scenarios: one with both typhoons and one with LEKIMA removed. Results reveal LEKIMA-induced distinctive atmospheric responses, including the closure of the western North Pacific subtropical high (WNPSH) boundary and the formulation of a wave train, influencing KROSA’s stagnation. The absence of LEKIMA allows KROSA to move more freely along the steering flow. Furthermore, the study highlights the potential of atmospheric models for understanding typhoon effects at regional to mesoscale levels. A comprehensive analysis of similar cases could enhance typhoon predictions, contributing to better damage mitigation strategies.

DEVELOPING A LOCALIZED OPTIMAL FLOOD BARRIER IN THE PHILIPPINES

In the Philippines, flood is a usual experience due to its geographical location near the equator. This calls for governmental units to establish flood control protocols that utilize equipment to effectively mitigate the impact of the floods. However, the existing protocols lack proper equipment integrated in their flood protocols that effectively handle flood occurrences. This research aims to improvise the current practices of the Philippine local government units in combating flood by proposing the optimal flood barrier design to be deployed for flood control and damage mitigation and investigate the optimal flood barrier for deployment. The methodology involves revisiting operational manuals of the target community of the Philippine governmental units and exploring existing flood barrier designs in literature and the market, whether in or out of the Philippines. Barriers designed in the literature and market were investigated, where there is a physical experiment conducted for the optimal design using physics concepts and past tests performed by studies. Results demonstrated the amount of force that can be resisted by the barriers, the amount of flood water until it floats, and the maximum velocity until barriers move. This is the first study to investigate the designs of flood barriers that are suitable for deployment and selects the optimal barrier for flood control for the Philippine setting while integrating it to the Philippine flood protocols. This research will be valuable to studies in regards with flood emergency disaster management planning.

Effect of Selenium and Selenoproteins on Radiation Resistance.

With the advancement of radiological medicine and nuclear industry technology, radiation is increasingly used to diagnose human health disorders. However, large-scale nuclear leakage has heightened concerns about the impact on human organs and tissues. Selenium is an essential trace element that functions in the body mainly in the form of selenoproteins. Selenium and selenoproteins can protect against radiation by stimulating antioxidant actions, DNA repair functions, and immune enhancement. While studies on reducing radiation through antioxidants have been conducted for many years, the underlying mechanisms of selenium and selenoproteins as significant antioxidants in radiation damage mitigation remain incompletely understood. Therefore, this paper aims to provide new insights into developing safe and effective radiation protection agents by summarizing the anti-radiation mechanism of selenium and selenoproteins.

Cyclic RGD conjugated PEG-PLGA nanoparticle enhances preventative effect of bilirubin on the development of ultraviolet-B (UVB)-induced cataract

Age related cataract (ARC) is characterized by protein conformational disorder with aging stands out as an independent risk factor. Therefore, mitigation of oxidative stress and DNA damage within the lens emerges as a potential therapeutic strategy. While topical administration remains the most common treatment modality for ocular disorder, drug bioavailability is hindered by the cornea epithelium barrier. In this study, we developed a novel cyclic Arg-Gly-Asp (cRGD)-conjugated nanoparticle encapsulating bilirubin (cRNM@BR), an endogenous antioxidant, to combat against cellular senescence and lens opacity associated with ARC. Immunofluorescence staining assay confirmed that cRGD modification significantly prolonged nanoparticle retention on the ocular surface by targeting integrin expressed on corneal epithelium. Moreover, through LC-MS/MS analysis, we demonstrated the enhanced transcorneal penetration efficacy of cRGD-modified nanodrugs both in vivo and in vitro. Our mechanistic investigations revealed that cRNM entered human corneal epithelial cells (HCECs) by clathrin and micropinocytosis-mediated pathways, with the ER/Golgi pathway facilitating its exocytosis. In a mouse model of ultraviolet B (UVB)-induced cataract, topical application of cRNM@BR effectively alleviated intracellular reactive oxygen species (ROS) levels and DNA damage in lens epithelial cells (LECs), thereby preventing cellular senescence and lens opacity. Transcriptomics analysis of LECs in UVB-exposed group and cRNM@BR pretreated group indicated significant enrichment of the cGAS/STING pathway. Further experiments determined that cRNM@BR prevented cellular senescence and lens opacity by ameliorating the cGAS/STING pathway. In conclusion, our findings underscore the therapeutic potential of cRNM@BR in ARC management and highlight the efficacy of cRGD-conjugated nanoplatform for intraocular delivery.

Diffusion distribution model for damage mitigation in scanning transmission electron microscopy.

Despite the widespread use of Scanning Transmission Electron Microscopy (STEM) for observing the structure of materials at the atomic scale, a detailed understanding of some relevant electron beam damage mechanisms is limited. Recent reports suggest that certain types of damage can be modelled as a diffusion process and that the accumulation effects of this process must be kept low in order to reduce damage. We therefore develop an explicit mathematical formulation of spatiotemporal diffusion processes in STEM that take into account both instrument and sample parameters. Furthermore, our framework can aid the design of Diffusion Controlled Sampling (DCS) strategies using optimally selected probe positions in STEM, that constrain the cumulative diffusion distribution. Numerical simulations highlight the variability of the cumulative diffusion distribution for different experimental STEM configurations. These analytical and numerical frameworks can subsequently be used for careful design of 2- and 4-dimensional STEM experiments where beam damage isminimised.

Therapeutic Function of Liraglutide for Mitigation of Blast-Induced Hearing Damage: An Initial Investigation in Animal Model of Chinchilla.

Auditory injuries induced by repeated exposures to blasts reduce the operational performance capability and the life quality of military personnel. The treatment for blast-induced progressive hearing damage is lacking. We have recently investigated the therapeutic function of liraglutide, a glucagon-like peptide-1 receptor agonist, to mitigate blast-induced hearing damage in the animal model of chinchilla, under different blast intensities, wearing earplugs (EPs) or not during blasts, and drug-treatment plan. The goal of this study was to investigate the therapeutical function of liraglutide by comparing the results obtained under different conditions. Previous studies on chinchillas from two under-blast ear conditions (EP/open), two blast plans (G1: 6 blasts at 3-5 psi or G2:3 blasts at 15-25 psi), and three treatment plans (blast control, pre-blast drug treatment, and post-blast drug treatment) were summarized. The auditory brainstem response (ABR), distortion product otoacoustic emission (DPOAE), and middle latency response (MLR) recorded within 14 days after the blasts were used. Statistical analysis was performed to evaluate the effect of liraglutide under different conditions. ABR threshold shifts indicated that the conditions of the EP and open ears were substantially different. Results from EP chinchillas indicated that the pre-blast treatment reduced the acute ABR threshold elevation on the day of blasts, and the significance of such an effect increased with the blast level. Liraglutide-treated open chinchillas showed lower ABR threshold shifts at the later stage of the experiment regardless of the blast levels. The DPOAE was less damaged after G2 blasts compared to G1 when pre-blast liraglutide was administrated. Lower post-blast MLR amplitudes were observed in the pre-blast treatment groups. This study indicated that the liraglutide mitigated the blast-induced auditory injuries. In EP ears, the pre-blast administration of liraglutide reduced the severity of blast-induced acute damage in ears with EP protection, especially under G2. In animals with open ears, the effect of liraglutide on the restoration of hearing increased with time. The liraglutide potentially benefits post-blast hearing through multiple approaches with different mechanics.

Amelioration effects of chlorogenic acid on mice colitis: Anti-inflammatory and regulation of gut flora

This research endeavors to investigate the potential preventive and protective effects of chlorogenic acid (CGA), a bioactive component found in various foods, in the context of dextran sodium sulfate (DSS)-induced colitis in mice. The study reveals that CGA administration, at dosages of 150 and 300 mg/kg, significantly retards the progression of colitis. This is substantiated by observable enhancements in colon length, reduction in the disease activity index, and mitigation of colon tissue damage. Meanwhile, CGA acts as an antioxidant by reducing the oxidative stress associated with colitis inflammation and increasing the activity of antioxidant enzymes. Furthermore, CGA demonstrates anti-inflammatory properties by inhibiting the inflammatory response associated with colitis and regulating the excessive expression of cytokines. Notably, CGA contributes to the restoration of intestinal barrier function by augmenting the levels of claudin-1 and ZO-1. Moreover, the investigation uncovers CGA's capacity to modulate the gut microbiota composition in colitic mice, fostering the proliferation of beneficial bacteria, notably Lactobacillus, while concurrently impeding the growth of detrimental bacteria like Bacteroides, Enterobacteriaceae, and Escherichia_Shigella. To conclude, these findings substantiate the viability of CGA for diverse applications in the realms of food and medicine, thereby bolstering its potential as a therapeutic strategy within dietary interventions.