Mite Attack Research Articles

Evaluation of the Sensitivity of Thirteen (13) Promising Cassava Varieties to Diseases (Viruses-Bacteria) and Pests (Mealybugs-Mites) in Central Côte d’Ivoire

Cassava (<i>Manihot esculenta</i>), a plant with starch-rich roots, plays a crucial role in global food security. For producers, it has become both a subsistence and cash crop. In Côte d’Ivoire, it ranks second among crops after yam. However, cassava is severely affected by bacteria, fungi, viruses, mites, and mealybugs, causing yield losses ranging from 20% to 90%. This study was conducted at the Food Crop Research Station (FCRS) of the National Center for Agronomic Research (NCAR) in Bouake in central Côte d’Ivoire. The plant material consisted of thirteen varieties of cassava of the <i>Manihot esculenta</i> species, from the national cassava collection. It aimed to assess the performance of thirteen promising cassava varieties, originating from the national cassava collection, against these diseases and pests under natural infestation conditions. The results revealed that the Yacé variety showed the highest sensitivity to viruses, with an incidence (I) of 96.25% and a severity index (IGS) of 2.8. In contrast, the Yavo variety was the least sensitive, with an incidence of 11.50% and a severity index of 1.3. All varieties studied demonstrated high sensitivity to bacterial blight and mite attacks, with high incidence rates observed in Bocou1 (71.25%) and Bocou3 (68.75%). However, mealybug infestation was relatively low, ranging from 8.75% (Yavo) to 33.75% (Bocou2). Except for mosaic disease, the severity indices for diseases and pests were similar across all varieties.

Metabolite profiling and transcriptome analyses reveal defense regulatory network against pink tea mite invasion in tea plant

BackgroundThe tea plant Camellia sinensis (L.) O. Kuntze is a perennial crop, invaded by diversity of insect pest species, and pink tea mite is one of the most devastating pests for sustainable tea production. However, molecular mechanism of defense responses against pink tea mites in tea is still unknown. In this study, metabolomics and transcriptome profiles of susceptible and resistant tea varieties were compared before and after pink tea mite infestation.ResultsMetabolomics analysis revealed that abundance levels of polyphenol-related compounds changed significantly before and after infestation. At the transcript level, nearly 8 GB of clean reads were obtained from each sequenced library, and a comparison of infested plants of resistant and susceptible tea varieties revealed 9402 genes with significant differential expression. An array of genes enriched in plant pathogen interaction and biosynthetic pathways of phenylpropanoids showed significant differential regulation in response to pink tea mite invasion. In particular, the functional network linkage of disease resistant proteins, phenylalanine ammonia lyase, flavanone -3-hydroxylase, hydroxycinnamoyl-CoA shikimate transferase, brassinosteroid-6-oxidase 1, and gibberellin 2 beta-dioxygenase induced dynamic defense signals to suppress prolonged pink tea mite attacks. Further integrated analyses identified a complex network of transcripts and metabolites interlinked with precursors of various flavonoids that are likely modulate resistance against to pink tea mite.ConclusionsOur results characterized the profiles of insect induced metabolic and transcript reprogramming and identified a defense regulatory network that can potentially be used to fend off pink tea mites damage.

Hyperspectral Imaging and Machine Learning: A Promising Tool for the Early Detection of Tetranychus urticae Koch Infestation in Cotton

Monitoring Tetranychus urticae Koch in cotton crops is challenging due to the vast crop areas and clustered mite attacks, hindering early infestation detection. Hyperspectral imaging offers a solution to such a challenge by capturing detailed spectral information for more accurate pest detection. This study evaluated machine learning models for classifying T. urticae infestation levels in cotton using proximal hyperspectral remote sensing. Leaf reflection data were collected over 21 days, covering various infestation levels: no infestation (0 mites/leaf), low (1–10), medium (11–30), and high (>30). Data were preprocessed, and spectral bands were selected to train six machine learning models, including Random Forest (RF), Principal Component Analysis–Linear Discriminant Analysis (PCA-LDA), Feedforward Neural Network (FNN), Support Vector Machine (SVM), k-Nearest Neighbor (kNN), and Partial Least Squares (PLS). Our analysis identified 31 out of 281 wavelengths in the near-infrared (NIR) region (817–941 nm) that achieved accuracies between 80% and 100% across 21 assessment days using Random Forest and Feedforward Neural Network models to distinguish infestation levels. The PCA loadings highlighted 907.69 nm as the most significant wavelength for differentiating levels of two-spotted mite infestation. These findings are significant for developing novel monitoring methodologies for T. urticae in cotton, offering insights for early detection, potential cost savings in cotton production, and the validation of the spectral signature of T. urticae damage, thus enabling more efficient monitoring methods.

Spider mite infestation triggers coordinated hormonal trade-offs enabling plant survival with a fitness cost.

Tetranychus urticae is an important pest that causes severe damage to a wide variety of plants and crops, leading to a substantial productivity loss. Previous research has been focused on plant defence response to T. urticae to improve plant resistance. However, plant growth, development and reproduction throughout the infestation process have not been previously studied. Through physiological, biochemical, transcriptomic and hormonomic evaluation, we uncover the molecular mechanisms directing the defence-growth trade-off established in Arabidopsis upon T. urticae infestation. Upon mite attack, plants suffer an adaptation process characterized by a temporal separation between the defence and growth responses. Jasmonic and salicylic acids regulate the main defence responses in combination with auxin and abscisic acid. However, while the reduction of both auxin signalling and gibberellin, cytokinin and brassinosteroid biosynthesis lead to initial growth arrest, increasing levels of growth hormones at later stages enables growth restart. These alterations lead to a plant developmental delay that impacts both seed production and longevity. We demonstrate that coordinated trade-offs determine plant adaptation and survival, revealing mite infestation has a long-lasting effect negatively impacting seed viability. This study provides additional tools to design pest management strategies that improve resistance without penalty in plant fitness.

Genetic determinism of lemon resistance to the two-spotted spider mite attack

Genetic determinism of lemon resistance to the two-spotted spider mite attack

QTL analysis on a lemon population provides novel insights on the genetic regulation of the tolerance to the two-spotted spider mite attack

BackgroundAmong the Citrus species, lemon (Citrus limon Burm f.) is one of the most affected by the two-spotted spider mite (Tetranychus urticae Koch). Moreover, chemical control is hampered by the mite’s ability to develop genetic resistance against acaricides. In this context, the identification of the genetic basis of the host resistance could represent a sustainable strategy for spider mite control. In the present study, a marker-trait association analysis was performed on a lemon population employing an association mapping approach. An inter-specific full-sib population composed of 109 accessions was phenotyped through a detached-leaf assays performed in modified Huffaker cells. Those individuals, complemented with two inter-specific segregating populations, were genotyped using a target-sequencing approach called SPET (Single Primer Enrichment Technology), the resulting SNPs were employed for the generation of an integrated genetic map.ResultsThe percentage of damaged area in the full-sib population showed a quantitative distribution with values ranging from 0.36 to 9.67%. A total of 47,298 SNPs were selected for an association mapping study and a significant marker linked with resistance to spider mite was detected on linkage group 5. In silico gene annotation of the QTL interval enabled the detection of 13 genes involved in immune response to biotic and abiotic stress. Gene expression analysis showed an over expression of the gene encoding for the ethylene-responsive transcription factor ERF098-like, already characterized in Arabidopsis and in rice for its involvement in defense response.ConclusionThe identification of a molecular marker linked to the resistance to spider mite attack can pave the way for the development of marker-assisted breeding plan for the development of novel selection coupling favorable agronomical traits (e.g. fruit quality, yield) with a higher resistance toward the mite.

Resistance of oil palm seedlings against red spider mite attack through boron fertilizer application

Resistance of oil palm seedlings against red spider mite attack through boron fertilizer application

Osmotin1 is involved in rice resistance to Schizotetranychus oryzae (Acari: Tetranychidae) infestation.

Rice is one of the most consumed cereals in the world. Productivity losses are caused by different biotic stresses. One of the most common is the phytophagous mite Schizotetranychus oryzae Rossi de Simons (Acari: Tetranychidae), which inhibits plant development and seed production. The identification of plant defense proteins is important for a better understanding of the mite-plant interaction. We previously detected a high expression of Osmotin1 protein in mite-resistant rice cultivars, under infested conditions, suggesting it could be involved in plant defense against mite attack. We therefore aimed to evaluate the responses of three rice lines overexpressing Osmotin1 (OSM1-OE) and three lines lacking the Osmotin1 gene (osm1-ko) to mite attack. The numbers of individuals (adults, immature stages, and eggs) were significantly lower in OSM1-OE lines than those in wild-type (WT) plants. On the other hand, the osm1-ko lines showed larger numbers of mites per leaf than WT plants. When plants reached the full maturity stage, two out of the three infested OSM1-OE lines presented lower plant height than WT, while the three osm1-ko lines (infested or not) presented higher plant height than WT. The reduction in seed number caused by mite infestation was lower in OSM1-OE lines (12-19%) than in WT plants (34%), while osm1-ko lines presented higher reduction (24-54%) in seed number than WT plants (13%). These data suggest that Osmotin1 is involved in rice resistance to S. oryzae infestation. This is the first work showing increased plant resistance to herbivory overexpressing an Osmotin gene. © 2023 Society of Chemical Industry.

Grapevine plant defense responses associated with arthropod herbivory: A review

Grapes are among the most valuable fruit crops with over ∼80 billion US dollars in farm gate value worldwide. Many insect pests, including phylloxera, mealybugs, grapevine moths, and spotted lanternfly, among others, persistently challenge grapevine plants. Insect infestations can limit berry production and affect the quality and quantity of its several byproducts, resulting in economic losses. To counter insect attack, grapevines employ different mechanisms, including structural, direct, indirect, and inducible defenses. Surprisingly, there is a lack of literature review in the grapevine-arthropod interactions study system. This review summarizes various defense mechanisms, including primary/secondary metabolites, elicitors, volatile organic compounds, etc., used by grapevines against insect and mite attacks that would help to improve and develop pest management strategies in vineyards. Finally, future perspectives of grapevine-insect interactions will be discussed, which could help to reveal novel grapevine defense mechanisms against arthropod herbivory and develop new pest management strategies to reduce crop losses.

Plant Growth-Promoting Rhizobacteria and Chabazite Zeolite in the growth stimulation and protection of Sedum palmeri and Sedum sieboldii from aphid and red spider mite attack

Research objective: The aim of this research was to evaluate the interaction that can develop between zeolite and microorganisms with beneficial effects on both the growth of Sedum palmeri and Sedum sieboldii and in the defence against Aphis sedii and Tetranychus urticae. Materials and Methods: The experiments, which started in January 2023, were conducted in the CREA-OF greenhouses in Pescia (Pt), Tuscany, Italy (43°54′N 10°41′E). The plants were placed in pots with a diameter of 12, 10 plants per 3 replications, for a total of 30 seedlings per experiment. The Sedum trial included the following theses (irrigated and fertilised): peat 70% + pumice 30% (CTRL); peat 70% + pumice 10% + chabazite natural zeolite 20%; peat 70% + pumice 30% + microorganisms (Azospirillum brasilense, Pseudomonas kilonensis and Bacillus aryabhattai), 1g/Kg of substrate at the time of substrate preparation; peat 70% + pumice 10% + chabazite natural zeolite 20% and micro-organisms (the same as those used in the thesis described above) in the same dosage. A second parallel experiment to control aphids and red spider mite involved: water treatment; treatment for aphids and red spider mite (soft potassium soap and azadirachtin 2.5 ml/l once a week; Kanemite 3 ml/l once a week); treatment for aphids and the alternative red spider mite (micronised zeolite 15g/l and microorganisms 1g/l, once a week). Plant height, leaves and flowers number, vegetative weight, root volume and length, microorganisms in the substrate, number of plants affected by aphids and red spider mite were determined on 15 September 2023. Results and Discussion: The experiment showed that the use of chabazite zeolite in combination with microorganisms can indeed significantly improve the vegetative and root growth of Sedum palmeri and Sedum sieboldii plants. Differences in growth between the use of zeolite and microorganisms and the untreated control are evident, with a significant improvement when the two theses are combined for all agronomic parameters analysed. In the substrate analysis, a greater presence of microbial biomass was found in the theses with zeolite, microorganisms and zeolite together with microorganisms than in the control theses, with a greater superiority of microorganisms in the substrate when zeolite and microorganisms are used in combination. The trial with micronised zeolite and microorganisms showed a significant result on the control of aphids and red spider mites, with data comparable to those of treatment with conventional chemical products. Zeolites are used successfully in the cultivation of many crops. Including cereals, vegetables, grapes and other fruits. By enhancing the absorption ability of soil, zeolite enhance the long-term quality of soil by retaining nutrients. Conclusions: For agricultural production, zeolites must have uniform properties and unique characteristics, such as cation exchange capacity and pH. Especially in agriculture and plant cultivation, zeolites improve their exchange capacity with the soil and plants through microbial activity. By lowering the pH of the substrate, microorganisms are able to solubilise the minerals retained by the zeolites, making them more available to the roots. Furthermore, zeolites, when micronised on the leaves, do a good job of protecting plants from insects and fungi, especially when used as a preventive measure. This can also ensure a significant reduction in synthetic chemicals and improve plant protection when these strategies are used in combination.

Screening of Suitable Castor Cultivars for Ericulture in Tamil Nadu, India

Although castor cultivation is predominant in Gujarat, Andhra Pradesh, Tamil Nadu, and few other states in India, rearing of eri silkworm, which feeds on castor is not catching up in these non-traditional states. One of the probable reasons is the non-availability of studies indicating region-specific suitable castor cultivars for different eri silkworm races. Studies on castor cultivar preference for one such better-performing race, C2 yielding better shell weight (12-15 Kg/100 DFL) in the Tamil Nadu region is lacking. Eleven castor cultivars, including local accessions, varieties, and hybrids, have been raised in Randomised Block Design (RBD) with three replicates. The worms were reared using all the grown cultivars with five replications for each cultivar (treatment) with 20 worms/replication. All the economic parameters of eri silkworm were computed and compared. Local accessions such as Krishnagiri local and Tindivanam local, Perennial castor - YTP – 1, and Hybrids such as YRCH – 1 and DCH – 519 recorded significantly better larval, cocoon, and reproductive parameters. Least larval duration and highest matured larval weight were recorded in Krishnagiri local accession. Least values of all the economic parameters were noticed in Dharmapuri local. The reason might be due to the poor leaf quality impacted due to severe whitefly (Trialeyrodes ricini) and red spider mite (Tetranychus urticae) attack.

Phenotyping and genotyping of a lemon segregating population to mining genetic resistance to the two-spotted spider mite attack

Phenotyping and genotyping of a lemon segregating population to mining genetic resistance to the two-spotted spider mite attack

Transcriptome analysis and identification of genes associated with leaf crude protein content in foxtail millet [Setaria italica (L.) P. Beauv.

Introduction: Spruce spider mite is a primary insect pest of Chinese chestnut in China and seriously influences its yield and quality. However, the current management against this mite is costly and poorly effective. In previous research, we bred several foxtail millet materials for interplanting with chestnut tree, and found that they had high levels of crude protein (CP) in leaves and attracted spruce spider mite to feed on the leaves, thereby reducing chestnut damage. Methods: In this study, four foxtail millet varieties with significant differences in leaf crude protein content were used for high-throughput sequencing and identification of genes associated with leaf crude protein content. Gene enrichment analyses were carried out to comprehend the functions of these genes and the biological processes in which they are involved. In addition, transcription factors (TFs) were evaluated. Results: 435 differentially expressed genes (DEGs) were identified, suggesting their potential role in crude protein accumulation. Some differentially expressed genes were found to be associated with nitrogen metabolism and ubiquitin-mediated proteolysis pathways. Moreover, we identified 40 TF genes categorized into 11 transcription factor families. Discussion: Our findings represent an important resource that clarifies the mechanisms of accumulation and control of leaf crude protein in foxtail millet, and provide an opportunity for suppression of spruce spider mite attack on Chinese chestnut by interplanting with foxtail millet varieties with high concentrations of leaf crude protein.

Effect of Biotic and abiotic factors on mites infestation under grain storage- A review

Mites are common pests of stored grain containing high fat and protein content. They are considered secondary invaders among the diverse range of storage pests because they are unable to infest sound grain and instead feed on fractured kernels, debris, and high moisture grain that has been already damaged by some insect pests. The high humidity and optimum temperature are determining factors for the sensitivity of the food grains for mites attack. Mites directly risk human health by contaminating food and indirectly by mycotoxin released under storage conditions. They also result in a considerable loss in grain weight and a reduction in germination ability. The losses become more severe as the mite population grows. Chemical agents have traditionally been used to prevent or manage mite infestations, as it is the simplest and most cost-effective approach to dealing with stored grain mites. This review paper discusses Distribution, pest status, host range, food grain choice, abiotic factor influence, quantitative damage, change in biochemical composition, and botanicals for mite management.

Investigation of the Potential to Introduce Internet of Things (Iot) Based Micro-Climate Controlling System for Salad Cucumber (Cucumis Sativus) in Conventional Protected Houses

Protected agriculture seeks to maximize productivity by manipulating climatic factors such as temperature to create the most favorable environmental conditions for the growth of animals and plants. This study aims to (a) identify the status of middle and small-scale protected house farmers using a questionnaire survey and (b) assess the impact of an IoT-based automation system to address identified issues of middle and small-scale protected house farmers. During the survey, the farmers revealed their inability to leave the polytunnel for at least one day without supervision and the higher labour cost as issues related to crop management. Decreased fruit quality and increased mite attacks, inability to achieve optimum crop yield were the main issues discovered which reduces the crop yield. The experiment was carried out in two protected houses where one house is under an IoT-based micro-climate controlling system and the conventional management in the other. Digital Temperature & Humidity Sensors (DHT21) were used to monitor relative humidity (RH) and temperature, and foggers and exhaust fans were used to control RH and temperature. Capacitance sensors were used to monitor substrate moisture, and a drip irrigation system was used to control it. Salad cucumber plants were grown in grow bags in each protected house. The inside day temperature was reduced by 1.55°C to 4.42°C in the IoT-based protected house and optimum inside day RH conditions were maintained. The maintained micro-climatic conditions showed a positive effect on yield and growth parameters of salad cucumber grown in IoT based protected house. In comparison to a conventional house, the IoT-based protected house had a 41.6% yield increment per vine.

Opposite roles of MAPKKK17 and MAPKKK21 against Tetranychus urticae in Arabidopsis.

After recognizing a biotic stress, plants activate signalling pathways to fight against the attack. Typically, these signalling pathways involve the activation of phosphorylation cascades mediated by Mitogen-Activated Protein Kinases (MAPKs). In the Arabidopsis thaliana-Tetranychus urticae plant-herbivore model, several Arabidopsis MAP kinases are induced by the mite attack. In this study, we demonstrate the participation of the MEKK-like kinases MAPKKK17 and MAPKKK21. Leaf damage caused by the mite was assessed using T-DNA insertion lines. Differential levels of damage were found when the expression of MAPKKK17 was increased or reduced. In contrast, reduced expression of MAPKKK21 resulted in less damage caused by the mite. Whereas the expression of several genes associated with hormonal responses did not suffer significant variations in the T-DNA insertion lines, the expression of one of these kinases depends on the expression of the other one. In addition, MAPKKK17 and MAPKKK21 are coexpressed with different sets of genes and encode proteins with low similarity in the C-terminal region. Overall, our results demonstrate that MAPKKK17 and MAPKKK21 have opposite roles. MAPKKK17 and MAPKKK21 act as positive and negative regulators, respectively, on the plant response. The induction of MAPKKK17 and MAPKKK21 after mite infestation would be integrated into the bulk of signalling pathways activated to balance the response of the plant to a biotic stress.

Incidence and post-embryonic development of Raoiella macfarlanei Pritchard and Baker (Acari: Tenuipalpidae) on Syzygium Jambos

The genus Raoiella represents one of the important genera of the family Tenuipalpidae, which comprises half a dozen species. Members of this group are known to infest a variety of economically important plants such as coconut, aereca nut, Eucalyptus, olive, peanut and various fruit crops. Among these, the species Raoiella macfarlanei Pritchard and Baker has been recorded on olive, Syzygium jambos and widely distributed in India and Africa.The report of the present study revealed that R. macfarlanei has been recognized as a minor pest and widely distributed in various localities of Kerala, India. A perusal of available literature shows a lack of information on its incidence and biology as it has been given the status of an economic species. But in the present study a high infestation was noticed in the field by the development of brownish patches on the infected leaves, causing severe damage to the host plant. Older leaves were more susceptible to mite attack than the younger ones. Maximum population of R. macfarlanei was found attained during the months of February, March and April. The study also revealed that R. macfarlanei completed its development from egg to adult within 16 days on the host plant, S. jambos.

Host plants contribute to the global pattern and diversification of herbivorous eriophyoid mites

Eriophyoid mites (Acari: Eriophyoidea) comprise approximately 5000 named species, leading to the most species-rich superfamily in the Acari. They are strictly herbivorous with a high level of host specificity, reflecting 89% of these mites attack only one or two congeneric host species (Yin et al., 2022).

Population dynamics of two-spotted mite, Tetranychus urticae Koch (Acari: Tetranychidae) in relation to weather parameters on strawberry

Two-spotted mite, Tetranychus urticae (Koch) is the most common and often the most severe pest of strawberries always resulting in considerable yield losses. The present research work aimed to investigate the effect of temperature, humidity, rainfall and sunshine hours on the population dynamics and severity of mite attack. A field experiment was carried out during April 2020 to March 2022 at Regional Horticultural Research & Training Station, Mashobra, Shimla, India. Mite population was determined by counting the number of active stages and eggs on the dorsal as well as on the ventral surface of the leaf under a binocular microscope. Two leaves were selected randomly from each plant and thus a total of 50 leaves per 25 plants were observed. The results of the studies indicated that population of two-spotted mite on strawberry fluctuated during April to June. Due to heavy rainfall (RF=101.0mm) received during 2020 in the first week of June mite count dropped to 3.20 active stages and 6.78 eggs/leaf. Thereafter, the mite population started increasing gradually in the subsequent weeks and it reached 18.60 active stages and 37.15 eggs/leaf by the end of June. During July-August mite population declined drastically due to washing off mites by continuous rainfall and mite count fluctuated between 0.85 to14.85 active stages and 0.83 to 27.80 eggs/leaf (Temp=17.16 to 19.28°C; RH=70.57 to 90.79%; RF=10.80 to 85.10 mm; 1.31 to 6.07 hrs) during 2020 while it reduced to minimum level of 0.30 active stages and 1.20 eggs/leaf during 2021 due to heavy rainfall (RF=143.40 mm). It multiplied with fast rate during dry months and a hike in mite count was seen from the second week of October with a peak in the fourth week of November during 2020 with the highest mean population of 89.45 active stages and 131.80eggs/leaf with mean temperature 13.51°C, 37.50% relative humidity, 0.00 mm rainfall and 8.11 hrs of sunshine. During 2021 a hike in the mite population was recorded in the fourth week of November with the highest mean population of 108.85 active stages and 186.65 eggs/leaf with mean temperature 13.05°C, 46.64% RH, 0.00 mm rainfall and 7.74 hrs of sunshine. Due to rain or snowfall received during January to February a sharp decline in mite population was observed during both seasons and it fluctuated between 1.45 to 8.35 active stages and 8.58 to 24.15 eggs/leaf (T=2.21 to7.65oC; RH=29.71 to 83.21%; RF=0.00 to 58.70 mm; SS=0.66 to 8.34 hrs). A slight hike in mite population was observed during March and mite count reached to 49.28 active stages and 76.89 eggs/leaf by the end of March, 2022 due to rise in mean temperature. The correlation coefficients (r) between mite population and temperature showed positive and significant relationship, whereas a significant but negative correlation existed between mite population and relative humidity and rainfall. The coefficient of determination between active stages and egg with weather parameters during studies ranged between 71% to 74.0% and 58 to 68.0%, respectively. Thus, the present investigations showed that the severity of mite attack on strawberry was the highest in temperature range of 13–18oC, 35–50% RH, zero rainfall, and during bright sunshine hours. These studies will help in forecasting the mite damage in advance for timely application of control measures.

The Behaviour of some Recommended Grapevine Cultivars in a Sustainable Crop System to the Eriophyid Mites Attack

The behaviour of some vine cultivars when attacked by the eriophyid mites,Colomerus vitis Pagenstecher which causes grapevine erinose and Calepitrimerus vitis Nalepa, the agent that causes acariosis, is extremely divers, and inconsistent. Some cultivars are resistant under certain conditions (locations), while others are more sensitive. The purpose of this study is to establish the resistance/tolerance of some cultivars to the attack of eryophyid mites, the main pests in the vineyards of Transylvania. The experiments were placed in the experimental plantations of SCDVV Blaj located in the Tarnave vineyard plantated with cultivars: Brumăriu, Fetească regală, Pinot gris, Traminer roz, Muscat Ottonel, Sauvignon blanc, Riesling italian, Blasius, Selena. By analysing the symptoms on the before mentioned cultivars, the attack degree (AD%) varied form 1% for Selena to 47% for Blasius. Among the cultivars, in 2020 Brumăriu has the highest AD% caused by Colomerus vitis with 18.82 %, compared with Pinot gris who had the lowest attack rate 0.08%. All the cultivars were midresistant and resistant against the eriophyid mites, with one exception, Brumăriu has an Attack Degree (AD %) of 18.82%, at the attack of Colomerus vitis.